Composite peptide for enhancing immunity, preparation method and application

Abstract

The invention discloses a composite peptide for enhancing immunity as well as a preparation method and application thereof, and relates to the technical field of health food. The composite raw materials comprise soybean peptide powder, crocodile peptide powder, sea cucumber peptide powder, tremella polysaccharide and honeysuckle flower powder; the peptide powder comprises the following raw materials in parts by weight: 28-61 parts of soybean peptide powder, 28-61 parts of crocodile peptide powder, 28-61 parts of sea cucumber peptide powder, 8-14 parts of tremella polysaccharide and 1.5-2 parts of honeysuckle flower powder. According to the composite peptide for enhancing the immunity, the preparation method and the application, the composite peptide has the effects of enhancing the immunity of the organism and resisting fatigue, and can promote the growth and development of thymus and spleen, improve the activity of superoxide dismutase in blood, reduce the activity of lactic dehydrogenase and the concentration of urea nitrogen and lactic acid which are related to fatigue; the exercise ability is enhanced; good application prospects are realized in the aspect of product development for enhancing the body immunity and the anti-fatigue effect.

Description

Technical Field

[0001] The present invention relates to the technical field of health food, and in particular to a composite peptide for enhancing immunity, a preparation method and application thereof. Background Art

[0002] Peptides are important substances involved in various cell functions in the human body. Peptides can synthesize cells and regulate cell functions. Peptides act as neurotransmitters in the human body to transmit information. Peptides can be used as a means of transport in the human body to transport various nutrients eaten by the human body, various vitamins, biotin, calcium and trace elements beneficial to the human body to various cells, organs and tissues of the human body. Peptides are important physiological regulators of the human body. They can comprehensively regulate the physiological functions of the human body, enhance and exert the physiological activity of the human body, and have important biological functions. Peptides are of irreplaceable importance to human cell activity, functional activities and life existence.

[0003] Peptides are compounds in which two or more amino acids are connected by peptide chains. They are functional nutrients between macromolecular proteins and small molecule amino acids, and are the most active, easily absorbed, and most stimulating to the human regeneration system. Peptides are irreplaceable materials that constitute active substances such as enzymes, hormones, antibodies, and interstitial nerves in the human body, and are the source of life. Peptides have unique physiological activities and medical and health care functions that original proteins and monomeric amino acids do not have, and have the triple functions of nutrition, health care, and treatment. There are many types of peptides, which can be roughly divided into three categories: animal peptides (collagen peptides, thymosin peptides, etc.), plant peptides (soy peptides, walnut peptides, etc.), chemically synthesized peptides, and drugs (oxytocin peptides, gastrin tetrapeptides, etc.).

[0004] Modern nutrition research has found that after the human body ingests protein, it is mostly digested and absorbed in the form of small peptides after the enzymes in the digestive tract, and the proportion absorbed in the form of free amino acids is very small. Further experiments have revealed that peptides are digested faster and absorbed more than free amino acids, indicating that the biological efficacy and nutritional value of peptides are higher than free amino acids. This is also the infinite charm of small molecule active peptides. The lack of active peptides in the human body can lead to diseases such as reduced immunity, indigestion, metabolic abnormalities, and growth and development disorders, and patients are difficult to recover. Scientists suggest that after meeting the basic nutritional intake of the human body, appropriate supplementation of active peptide nutrition is of great benefit to health and disease recovery.

[0005] Immunity is the body's own defense mechanism. It is the body's ability to identify and eliminate any foreign bodies (viruses, bacteria, etc.) that invade from outside, to deal with aging, damaged, dead, and degenerated self-cells, and to identify and deal with mutant cells and virus-infected cells in the body. A body with low immunity is prone to infection or cancer, and abnormal immunity can also produce harmful results for the body, such as allergic reactions and autoimmune diseases. Various reasons prevent the immune system from playing a protective role normally. In this case, it is very easy to be infected by bacteria, viruses, fungi, etc. The manifestation is that it is easy to get sick. Because of frequent illness, the body's consumption is aggravated, so there are generally physical weakness, malnutrition, mental depression, fatigue, loss of appetite, sleep disorders, etc.

[0006] Modern immunology believes that improving immunity is a physiological reaction of the human body to identify and eliminate "foreigners". The immune system performs this function in the human body. There are many ways to enhance immunity, such as eating more beneficial foods. Children in particular should pay more attention to enhancing immunity.

[0007] However, the complex peptides provided in the prior art have single nutritional ingredients, few functional factors, difficult to exert synergistic advantages, poor absorption and utilization, etc., and are not effective in improving the body's immunity, quickly replenishing nutrition and relieving fatigue. Summary of the invention

[0008] The present invention provides a composite peptide for enhancing immunity, a preparation method and an application thereof, which solve the problems that the existing composite peptide has a single nutritional component, few functional factors, difficulty in exerting the advantages of synergistic effects, poor absorption and utilization, and has no obvious effect on improving the body's immunity, quickly supplementing nutrition and relieving fatigue.

[0009] To achieve the above purpose, on the one hand, the present invention provides a composite peptide for enhancing immunity, the raw materials of which include: soybean peptide powder, crocodile peptide powder, sea cucumber peptide powder, tremella polysaccharide, and honeysuckle pollen.

[0010] Preferably, the raw material composition includes, by weight: 28-61 parts of soybean peptide powder, 28-61 parts of crocodile peptide powder, 28-61 parts of sea cucumber peptide powder, 8-14 parts of tremella polysaccharide, and 1.5-2 parts of honeysuckle pollen.

[0011] Preferably, the raw material composition includes, by weight: 28 parts of soybean peptide powder, 28 parts of crocodile peptide powder, 28 parts of sea cucumber peptide powder, 14 parts of tremella polysaccharide, and 2 parts of honeysuckle pollen.

[0012] On the other hand, the present invention provides a method for preparing the above-mentioned composite peptide for enhancing immunity, which is prepared by uniformly mixing all raw materials.

[0013] In another aspect, the present invention provides a use of the above-mentioned immunity enhancing composite peptide in preparing food.

[0014] In another aspect, the present invention provides a food containing the above-mentioned immunity-enhancing composite peptide.

[0015] Preferably, the food is a liquid beverage, a solid beverage, a biscuit, a meal replacement powder, a rice porridge or other prepared food.

[0016] In another aspect, the present invention provides a use of the above-mentioned immunity-enhancing composite peptide in the preparation of health products.

[0017] Sea cucumber peptide has multiple functions: (1) It is rich in peptides and amino acids, which can enhance the function of the human immune system and improve the body's resistance; (2) Contains protein peptides that are easy to digest and absorb, which can help improve the function of the digestive system and promote the absorption of nutrients; (3) Rich in collagen and amino acids, it can promote skin cell regeneration, maintain skin elasticity and radiance, and help delay skin aging; (4) Helps regulate blood sugar levels and lower blood sugar; (5) Rich in minerals such as calcium, zinc, and iron, it can help promote bone health, increase bone density, and prevent bone problems such as osteoporosis.

[0018] Crocodile peptide is an active substance extracted from crocodiles, which has the following functions: (1) Contains a variety of active ingredients, such as peptides and amino acids, which can stimulate cell regeneration and repair, promote collagen synthesis, and thus replenish qi and blood; crocodile peptide can regulate the activity of immune cells, enhance the function of the immune system, improve the body's resistance, and reduce the risk of infection and disease; (2) It has strong antioxidant capacity, can neutralize free radicals in the body, reduce oxidative damage to cells, and thus delay the aging process; (3) It can improve memory loss and enhance learning ability; its antioxidant properties can maintain the normal state of nerve cells, reduce the damage of oxidative stress to nerve cells, thereby slowing down the aging process of nerve cells and reducing the risk of neurodegenerative diseases in the elderly; (4) Promote blood circulation, efficiently transport nutrients to skin cells, and keep the skin young; (5) It has a small molecular weight, is extremely active and diverse, and can be actively absorbed by the human body without digestion; it can quickly pass through the mouth and stomach, enter the small intestine and be absorbed, and eventually enter the blood circulation system, organs and cell tissues, quickly exerting its physiological effects and biological functions.

[0019] Tremella polysaccharide is the main active ingredient of Tremella, which has a wide range of pharmacological effects, can activate the activity of various immune cells, and improve the body's anti-tumor ability. Honeysuckle contains a variety of effective chemical components, which have antibacterial, antipyretic, free radical scavenging, and liver damage protection effects.

[0020] The present invention provides a composite peptide for enhancing immunity, a preparation method and an application thereof, which have the following technical effects: (1) Experimental tests have found that the compound peptide powder can promote the growth and development of the thymus and spleen of mice, improve the body's immune function, and reduce alanine aminotransferase activity and blood sugar concentration. It has the effects of protecting the liver, lowering blood sugar, and resisting fatigue.

[0021] (2) Experimental tests found that the compound peptide powder reduced the concentration of fatigue-related indicators urea nitrogen and lactic acid in the blood of mice, while at the same time significantly counteracting the production of free radicals, helping to prevent cell membrane lipid peroxidation, thereby enhancing the mice's athletic ability.

[0022] (3) The composite peptide of the present invention has significant effects such as anti-oxidation and free radical scavenging, and the peptide has a small molecular weight and is easy to absorb.

[0023] (4) The preparation method is simple and can be mixed into daily solvents, making it easy to use.

[0024] (5) The composite peptide powder of the present invention has good application prospects in the development of products that enhance the body's immunity and anti-fatigue effects.

[0025] The technical solution of the present invention is further described in detail below through embodiments. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical scheme and advantages disclosed in the embodiments of the present invention clearer, the embodiments of the present invention are further described in detail in conjunction with the embodiments below. It should be understood that the specific embodiments described herein are only used to explain the embodiments of the present invention and are not intended to limit the embodiments of the present invention. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection of this application.

[0027] It should be noted that the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or server that includes a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units that are not explicitly listed or inherent to these processes, methods, products or devices.

[0028] Embodiment 1 A composite peptide for enhancing immunity, comprising: 0.1g soybean peptide powder, 0.1g crocodile peptide powder, 0.1g sea cucumber peptide powder, 0.05g tremella polysaccharide, 0.01g honeysuckle pollen, and the mixture is mixed evenly.

[0029] Embodiment 2 A composite peptide for enhancing immunity, comprising: 0.2g of soybean peptide powder, 0.2g of crocodile peptide powder, 0.2g of sea cucumber peptide powder, 0.10g of tremella polysaccharide, and 0.02g of honeysuckle pollen, and the mixture is mixed evenly.

[0030] Embodiment 3 A composite peptide for enhancing immunity, comprising: 0.3g soybean peptide powder, 0.3g crocodile peptide powder, 0.3g sea cucumber peptide powder, 0.15g tremella polysaccharide, 0.03g honeysuckle pollen, and the mixture is mixed evenly.

[0031] Embodiment 4 A composite peptide for enhancing immunity, comprising: 0.4g soybean peptide powder, 0.4g crocodile peptide powder, 0.4g sea cucumber peptide powder, 0.20g tremella polysaccharide, 0.04g honeysuckle pollen, and the mixture is mixed evenly.

[0032] Embodiment 5 A composite peptide for enhancing immunity, comprising: 0.4g soybean peptide powder, 0.1g crocodile peptide powder, 0.1g sea cucumber peptide powder, 0.05g tremella polysaccharide, 0.01g honeysuckle pollen, and the mixture is mixed evenly.

[0033] Embodiment 6 A composite peptide for enhancing immunity, comprising: 0.1g of soybean peptide powder, 0.4g of crocodile peptide powder, 0.1g of sea cucumber peptide powder, 0.05g of tremella polysaccharide, and 0.01g of honeysuckle pollen, and the mixture is mixed evenly.

[0034] Embodiment 7 A composite peptide for enhancing immunity, comprising: 0.1g soybean peptide powder, 0.1g crocodile peptide powder, 0.4g sea cucumber peptide powder, 0.05g tremella polysaccharide, 0.01g honeysuckle pollen, and the mixture is mixed evenly.

[0035] Embodiment 8 A composite peptide beverage for enhancing immunity comprises, by weight, 10 parts of soybean peptide powder, 10 parts of crocodile peptide powder, 10 parts of sea cucumber peptide powder, 5 parts of tremella polysaccharide, 1 part of honeysuckle pollen, 1 part of auxiliary materials, and the balance is water.

[0036] The auxiliary materials include: calculated by weight, 0.5 parts of maltitol, 0.1 parts of passion fruit essence, 0.1 parts of mogroside, 0.1 parts of stevioside, and 0.2 parts of citric acid monohydrate.

[0037] The preparation method comprises the following steps: S1. Predissolution: Pour the composite peptide composition and all auxiliary materials into a mixing barrel, add an appropriate amount of purified water to stir and dissolve, then transfer the liquid to a liquid mixing tank, and then rinse the mixing barrel with purified water for multiple times, and transfer the rinse liquid to the liquid mixing tank.

[0038] S2. Liquid preparation: Add purified water to the liquid preparation tank until the total weight of the liquid is 100 kg, and stir evenly.

[0039] S3, filtration: pass the stirred liquid through a 200-mesh filter, and transfer the filtrate to a liquid storage tank for standby use.

[0040] S4. Filling: Fill the liquid in the storage tank into the sterilized beverage bottle.

[0041] S5. Sterilization: Sterilize the filled beverage bottles at 115℃ for 15 minutes.

[0042] S6. Light inspection: After cooling down (below 40°C), the beverage bottles are subjected to light inspection to remove products with foreign matter inside.

[0043] S7, packaging, inspection and warehousing.

[0044] Test Example 1 Mouse grouping and immune capacity testing 40 male Kunming mice, each weighing 20-22 g, were randomly divided into 5 groups, each with 10 mice, including a control group and groups 1 to 4 of Examples, and each group was gavaged with 10 mL of the drug. The control group was given 10 mL of saline, and the experimental group was given the composite peptide dissolved in 10 mL of saline.

[0045] Feeding conditions: indoor temperature 21-22℃, humidity 60%-65%, day and night cycle of 12h light / 12h dark. The control group was given continuous gavage with the polypeptide complex group for 20 days. After weighing on the 21st day, the mice were fasted for 8h, blood was collected from the eyeballs, and routine serum biochemical indicators were detected using an automatic biochemical detector. The mice were killed by dislocation and the liver, spleen and thymus were removed. The liver index, spleen index and thymus index were calculated respectively (visceral index = visceral weight / body weight × 100%).

[0046] All experimental operations were carried out in strict accordance with the animal ethics regulations established by the Laboratory Animal Center.

[0047] Test Example 2 The mice were fed, grouped and gavaged according to the method in Test Example 1. On the 21st day, 30 minutes after the test substance was administered, the mice were placed on a vertically suspended glass rod to make their muscles tense. The time it took for the mice to fall from the glass rod was recorded. The experiment was terminated when the mice fell for the third time, and the total time accumulated for the three times was taken as the climbing time.

[0048] Test Example 3 The mice were fed, grouped and gavaged according to the method in Test Example 1. On the 21st day, 30 minutes after the test substance was administered, the mice were loaded with a weight of 5% of their body weight and placed in a swimming box with a water depth of not less than 30 cm and a water temperature of (25±1)°C. The time from the start of swimming to the time when the head was completely submerged in the water and could not float to the surface after 8 seconds was recorded as the mouse swimming time.

[0049] Test Example 4 According to the method in Test Example 1, 60 mice were raised and randomly divided into 6 groups, each with 10 mice, namely, a blank control group (normal saline), a milk powder control group (the compound peptide powder was replaced with the same amount of milk powder as the compound peptide powder in Example 1), and the compound peptide powder groups of Examples 1 to 4. After 5 days of adaptive feeding, the mice were subjected to gavage experiments, once a day, 0.5 mL each time, for 20 days, and weighed every 5 days. On the 21st day, each group of mice was placed in (25±1)℃ warm water to swim exhaustively, and then 1mL of blood was taken from the eyeball, centrifuged at 3000g for 5 minutes, and stored at -80℃ after aliquoting. The concentrations of urea nitrogen and lactic acid in the serum of mice and the activities of lactate dehydrogenase and superoxide dismutase were determined using the corresponding kits.

[0050] Milk powder was purchased from Wandashan Co., Ltd. Lactate dehydrogenase (LDH), superoxide dismutase (SOD), urea nitrogen (BUN) and lactic acid (LA) detection kits were purchased from Nanjing Jiancheng Biological Company.

[0051] Results Analysis The data were expressed as mean ± standard deviation and the t-test was performed using SPSS 24.0 software. p <0.05 indicates significant differences among different groups. p <0.01 indicated a very significant difference.

[0052] (1) Effect of compound peptide powder on the immune effect of mice The results of serum routine biochemical indexes are shown in Table 1. The results show that: Compared with the control group, the serum albumin, total protein and blood calcium levels of the experimental group of mice administered with polypeptide powder were significantly increased ( p <0.05, p <0.01).

[0053] The activity of alanine aminotransferase in the serum of mice in the different dose groups of polypeptide powder was significantly lower than that in the control group ( p <0.05, p <0.01), and the changes showed a certain concentration dependence.

[0054] The blood glucose concentration of mice in different dose groups of polypeptide powder was significantly lower than that in the control group ( p <0.05), while other indicators showed no significant changes.

[0055] Table 1 Effects of compound peptide powder on routine biochemical indices in mouse serum ; Note:*, p <0.05; **, p <0.01.

[0056] The results of visceral index detection are shown in Table 2. The results show that the spleen gland index and thymus gland index of the mice in the composite peptide groups tested in Examples 1 to 4 were significantly higher than those in the control group ( p <0.05, p <0.01); however, there was no significant difference between the liver index of mice treated with different doses of the composite peptides in Examples 1 to 4 and the control group.

[0057] Table 2 Effects of compound peptide powder on liver, thymus and spleen indexes of mice ; Note:*, p <0.05; **, p <0.01.

[0058] (2) Effects of compound peptide powder on the motor ability of mice As shown in Table 3, the results showed that after mice took different doses of compound peptide powder, the vertical climbing time and weighted swimming time were significantly longer than those of the control group ( p <0.05, p <0.01), and showed a significant dose effect.

[0059] Table 3 Effects of compound peptide powder on vertical climbing pole and weighted swimming time of mice ; Note:*, p <0.05; **, p <0.01.

[0060] Among them, the vertical climbing pole and weighted swimming time of the mice in the group with a higher dose of the composite peptide powder in Example 4 reached (29.24±3.31) min and (32.59±4.37) min, respectively, which were extended by 45.26% and 39.75% compared with the control group. The above results show that the composite peptide powder helps to improve the athletic ability of mice.

[0061] (3) Effect of compound peptide powder on the anti-fatigue effect of exhaustive swimming mice As shown in Table 4, the results showed that after exhaustive swimming, the urea nitrogen concentration and lactate dehydrogenase activity in the serum of mice in the milk powder control group were significantly lower than those in the blank control group ( p <0.05, p<0.01), while the differences in serum lactate concentration and superoxide dismutase activity were not significant; after exhaustive swimming, the urea nitrogen, lactate concentration and lactate dehydrogenase activity of the mice treated with different doses of the composite peptides in Examples 1 to 4 were significantly lower than those of the blank control group ( p <0.05, p <0.01).

[0062] The above indicators in the serum of mice treated with the lower dose of composite peptide in Examples 1 and 2 were not significantly different from those in the milk powder control group, while those in the high dose group of composite peptide powder in Examples 3 and 4 were significantly lower than those in the milk powder control group ( p <0.05); the superoxide dismutase activity in the serum of mice treated with different doses of the composite peptides in Examples 1 to 4 was significantly increased compared with that in the blank control group ( p <0.05, p <0.01), among which the mice in the high-dose group of compound peptide powder in Examples 3 and 4 had significantly higher levels than those in the milk powder control group ( p <0.05).

[0063] Table 4 Effects of compound peptide powder on related indicators in serum of exhausted swimming mice ; Note: *, compared with the blank control group, p <0.05; **, compared with the blank control group, p <0.01; #, compared with the milk powder control group, p <0.05.

[0064] The thymus is an important immune organ and plays a central role in the body's immune system. The thymosin and many other hormone-like substances it secretes help to improve the body's immunity. The spleen is the largest peripheral lymphoid organ in the human body and has hematopoietic and blood filtration functions. It is also an important place for lymphocytes to migrate and respond to antigenic stimulation and produce immune effector molecules.

[0065] Albumin and total protein in serum are closely related to the body's immune function. The above test found that the spleen index and thymus index of mice fed with compound peptide powder were significantly increased compared with those of control mice, indicating that compound peptide powder can promote the growth and development of mouse thymus and spleen; at the same time, in the serum biochemical indicators of mice fed with medium and high doses of compound peptide powder, the concentrations of albumin and total protein were significantly increased compared with those of control mice, indicating that compound peptide powder can improve the body's immune function.

[0066] The activity of alanine aminotransferase and blood glucose concentration in the serum of mice taking compound peptide powder were significantly lower than those of control mice, indicating that compound peptide powder has the effect of protecting liver and lowering blood sugar. Studies have found that indicators such as alanine aminotransferase activity, aspartate aminotransferase activity, and glucose content can be used as key indicators for evaluating fatigue. Combined with the analysis of test case results, it is suggested that compound peptide powder may have anti-fatigue effects.

[0067] Lactic acid is an intermediate product of glucose metabolism in the body. When exercise is relatively excessive, the lactic acid produced by muscles cannot be further decomposed in a short period of time. At the same time, insufficient oxygen supply will lead to the accumulation of a large amount of lactic acid. In addition, when the body cannot obtain the necessary energy to maintain the body from carbohydrates and fats, proteins and amino acids can be used as alternative energy sources, but the related catabolism will produce a large amount of urea nitrogen, which will further affect the contractility of muscles, and fatigue will occur in severe cases. Therefore, the degree of fatigue of the body can be evaluated by measuring the concentration of urea nitrogen and lactic acid in the blood.

[0068] Intense exercise can easily produce excessive free radicals, which can damage muscle cells and cause lactate dehydrogenase to infiltrate into the plasma. Lactate dehydrogenase is an important enzyme involved in anaerobic glycolysis and gluconeogenesis, which can promote the redox reaction between pyruvate and lactate; superoxide dismutase is a very important antioxidant enzyme that removes oxygen free radicals in the body. Therefore, studying the effect of peptides on the changes in serum urea nitrogen, lactic acid concentration, and lactate dehydrogenase and superoxide dismutase activity in mice after exercise is helpful to explore the anti-fatigue mechanism of peptides at the biochemical level.

[0069] The test case found that after exhaustive swimming, the concentrations of urea nitrogen and lactic acid in the serum of mice fed with compound peptide powder were significantly lower than those of blank control mice, the activity of lactate dehydrogenase was extremely significantly lower than that of blank control mice, and the activity of superoxide dismutase was significantly higher than that of blank control mice; the concentrations of urea nitrogen, lactic acid and lactate dehydrogenase in the serum of mice fed with low-dose compound peptide powder were not significantly different from those of control mice fed with milk powder, while those of mice fed with compound peptide powder were significantly lower. At the same time, the activity of superoxide dismutase in the serum of mice fed with high-dose compound peptide powder was significantly higher than that of control mice fed with milk powder. It can be seen that compound peptide powder reduces the concentrations of urea nitrogen and lactic acid, fatigue-related indicators in the blood of mice, and at the same time can significantly counteract the production of free radicals, helping to prevent cell membrane lipid peroxidation, thereby enhancing the exercise capacity of mice.

[0070] In summary, the present invention provides a composite peptide for enhancing immunity, a preparation method and an application thereof. The composite peptide has the function of enhancing the body's immunity and anti-fatigue effect, can promote the growth and development of the thymus and spleen, increase the activity of superoxide dismutase in the blood, reduce the activity of lactate dehydrogenase and the concentrations of fatigue-related indicators urea nitrogen and lactic acid, and enhance athletic ability; and has good application prospects in the development of products for enhancing the body's immunity and anti-fatigue effect.

[0071] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A composite peptide for enhancing immunity, characterized in that: Ingredients include: Soy peptide powder, crocodile peptide powder, sea cucumber peptide powder, Tremella polysaccharide, honeysuckle powder; Calculated by weight, the raw material composition includes: 28-61 parts of soybean peptide powder, 28-61 parts of crocodile peptide powder, 28-61 parts of sea cucumber peptide powder, 8-14 parts of tremella polysaccharide, and 1.5-2 parts of honeysuckle pollen.

2. The immune-enhancing composite peptide according to claim 1, characterized in that: Calculated by weight, the raw material composition includes: 28 parts of soybean peptide powder, 28 parts of crocodile peptide powder, 28 parts of sea cucumber peptide powder, 14 parts of tremella polysaccharide, and 2 parts of honeysuckle pollen.

3. A method for preparing the composite peptide for enhancing immunity as claimed in claim 1 or 2, characterized in that: Mix all the ingredients well.

4. Use of the immunity-enhancing composite peptide as claimed in claim 1 or 2 in preparing food.

5. A food containing the immunity-enhancing composite peptide according to claim 1 or 2.

6. The food according to claim 4, characterized in that: Food includes liquid beverages, solid beverages, biscuits, meal replacement powder, and rice porridge.

7. Use of the immunity enhancing composite peptide according to claim 1 or 2 in the preparation of health products.