Plant extract composition with Nrf2 excitation as well as preparation method and application of plant extract composition

Abstract

The invention discloses a plant extract composition with Nrf2 excitation as well as a preparation method and application of the plant extract composition. Belongs to the technical field of skin care products. The traditional Chinese medicine composition is prepared from the following components in percentage by weight: 0.1 to 1.0 percent of ginger root extract, 0.1 to 1.0 percent of ormosia henryi bark extract and 0.1 to 1.0 percent of saffron crocus extract. The plant extract composition with the Nrf2 excitation can obviously activate an Nrf2 target spot, scavenge free radicals, reduce ROS (reactive oxygen species) and realize oxidation resistance; the composition also has the effects of remarkably reducing the melanin content and improving the skin brightness. The core active components of the three plant extracts are all natural fat-soluble molecules, and have the advantages of being high in safety, free of irritation and high in efficiency, so that the plant extracts are easily applied to external local preparations of oil skin care products.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and specifically relates to a plant extract composition with Nrf2 excitation, a preparation method and use thereof. Background Art

[0002] The skin is the largest organ in the human body and is susceptible to external stimuli and endogenous aging, which can produce excessive free radicals. On the one hand, excessive free radicals can cause carbonylation and saccharification of protein lipids on the surface of the skin, resulting in uneven skin color. On the other hand, excessive free radicals can aggravate the cross-linking polymerization of collagen and accelerate the degradation of elastic fibers, leading to decreased skin tension, loss of elasticity and softness, and wrinkles, dryness and keratinization, and roughness and dullness of the face. It can be seen that oxidative damage caused by free radicals can cause serious damage to the skin.

[0003] Nrf2 is the core target of antioxidant activity. Nrf2 belongs to the CNC regulatory protein family and is a transcription factor with an alkaline leucine zipper structure. It is widely present in the skin and is the master regulator of cellular redox reactions. Under normal physiological conditions, Nrf2 mainly binds to its inhibitor Keap1 and exists in the cytoplasm in an inactive state. It is rapidly degraded under the action of the ubiquitin proteasome pathway to maintain the low transcriptional activity of Nrf2 under physiological conditions. When cells are stimulated by reactive oxygen species (ROS) or other nucleophiles, Nrf2 uncouples from Keap1, and the activated Nr2 is transported into the nucleus, binds to Maf protein to form a heterodimer, and then binds to ARE to activate target gene expression, regulate the transcriptional activity of phase II metabolic enzymes, antioxidant enzymes or drug transporters, and thus play an antioxidant role.

[0004] Turmeric is a perennial herb of the genus Curcuma in the ginger family. The main active ingredients of its roots are turmericone and curcumin. Because of its significant antioxidant effect, it has been widely used in history. Turmeric can relieve alcohol and prevent hangovers. Ancient Asians regarded it as a panacea, like a treasure, with high application value. The rosewood is an evergreen tree of the genus Rhododendron in the leguminous family. Its bark is rich in a stilbene polyphenol compound, pterostilbene, which is a derivative of resveratrol and has rich medicinal value and antioxidant activity. Saffron is an herb of the genus Crocus in the Iridaceae family. From the ancient Roman period to the present, it has been used in medicine and diet therapy to varying degrees. It mainly contains crocin and crocetin, and has high antioxidant activity.

[0005] Turmeric, pterostilbene and crocetin all have strong antioxidant effects, but they are all oil-soluble substances, which makes them difficult to use in general cosmetics and difficult to exert their unique antioxidant effects. Skin care oils themselves are similar to the components of sebum membranes, and are more easily absorbed by the skin, achieving better skin care effects. However, at present, there are still few products on the market for antioxidant skin care oils. Few studies have focused on oil-soluble antioxidant ingredients, which greatly limits the application of oil-soluble skin care products. Summary of the invention

[0006] In view of the above problems existing in the prior art, the purpose of the present invention is to design and provide a plant extract composition with Nrf2 excitation and a preparation method and a technical solution for its use. The plant extract composition can significantly activate the Nrf2 target, scavenge free radicals, reduce ROS, and achieve anti-oxidation; the composition can also significantly reduce the melanin content and improve the brightness of the skin. The core active ingredients of the three plant extracts are all fat-soluble molecules of natural origin, which have the advantages of high safety, non-irritation and high efficiency, and are therefore easily applied to topical preparations for external use of oil-based skin care products.

[0007] The plant extract composition with Nrf2 excitation is characterized by containing the following components in percentage by weight: 0.1-1.0% ginger root extract, 0.1-1.0% tamarisk bark extract, and 0.1-1.0% saffron extract.

[0008] The plant extract composition with Nrf2 stimulation is characterized in that the ginger root extract contains more than 65% of turmeric ketones, the Pterocarpus tataricus bark extract contains more than 80% of pterostilbene, and the saffron extract contains more than 30% of crocetin.

[0009] The plant extract composition with Nrf2 excitation is characterized by also containing: 42-45% caprylic / capric triglyceride, 30% squalane, 24% jojoba oil, and 0.7-1% glyceryl behenate.

[0010] The method for preparing a plant extract composition with Nrf2 excitation is characterized by comprising the following steps: heating 42-45% caprylic / capric triglyceride, 30% squalane, 24% jojoba oil, 0.7-1% glyceryl behenate, 0.1-1.0% ginger root extract, 0.1-1.0% tamarisk bark extract, and 0.1-1.0% saffron extract to 80°C, maintaining the temperature at 60-80°C and stirring for 1 hour to obtain the composition.

[0011] The invention discloses an application of the plant extract composition with Nrf2 excitation in the preparation of oil cosmetics.

[0012] The invention discloses an application of the plant extract composition with Nrf2 excitation in the preparation of oil cosmetics with antioxidant regulation, strong antioxidant, whitening and skin lightening effects.

[0013] The present invention has found through research that turmeric root extract has a strong free radical scavenging effect, and scavenges free radicals at the early stage of oxidative stress; pterostilbene can promote the formation of antioxidant enzymes in the body and help remove the formed peroxides; and saffron extract can protect cell DNA. These three components achieve comprehensive antioxidant in the antioxidant pathway mechanism.

[0014] The above-mentioned Nrf2-stimulating plant extract composition can significantly activate Nrf2 targets, scavenge free radicals, reduce ROS, and achieve anti-oxidation; the composition can also significantly reduce melanin content and improve skin brightness. The core active ingredients of the three plant extracts are all fat-soluble molecules of natural origin, which have the advantages of high safety, non-irritation, and high efficiency, and are therefore easily applied to topical preparations for external use of oil-based skin care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a summary diagram of the Nrf2 immunofluorescence results in Test 1 of the present invention;

[0016] Figure 2 It is a bar graph of the relative integrated optical density (IOD) value of Nrf2 in Test 1 of the present invention;

[0017] Figure 3 This is a summary of the ROS images in Test Example 2 of the present invention;

[0018] Figure 4 It is a bar graph of the ROS integrated optical density (IOD) value in Test Example 2 of the present invention;

[0019] Figure 5 This is a summary diagram of the melanin distribution detection results in Test Example 3 of the present invention;

[0020] Figure 6 This is a histogram of melanin distribution detection in Test Example 3 of the present invention;

[0021] Figure 7 This is a graph showing the effect of the apparent chromaticity test in Test Example 4 of the present invention;

[0022] Figure 8 This is a bar graph of the L* value detection results in Test Example 4 of the present invention. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0024] Example 1

[0025] 0.1% of turmeric root extract, 0.1% of thuja sutchuenensis bark extract and 0.1% of saffron extract were added to caprylic / capric triglyceride and stirred until completely dissolved to obtain extract composition 1.

[0026] Example 2

[0027] 0.5% of turmeric root extract, 0.5% of thuja sutchuenensis bark extract, and 0.5% of saffron extract were added to caprylic / capric triglyceride and stirred until completely dissolved to obtain extract composition 2.

[0028] Example 3

[0029] 1.0% of turmeric root extract, 1.0% of thuja sutchuenensis bark extract, and 1.0% of saffron extract were added to caprylic / capric triglyceride and stirred until completely dissolved to obtain extract composition 3.

[0030] Example 4

[0031] The following ingredients were heated to 80° C. and stirred while maintaining the temperature at 65° C. for 1 hour to obtain Composition 4.

[0032] Element content(%) Caprylic / capric triglyceride 45 Squalane 30 Jojoba Oil 24 Glyceryl Behenate 0.7 Palmyra tamarindus bark extract 0.1 Ginger Root Extract 0.1 Saffron Extract 0.1

[0033] Example 5

[0034] The following ingredients were heated to 80° C. and stirred while maintaining the temperature at 60° C. for 1 hour to obtain Composition 5.

[0035] Element content(%) Caprylic / capric triglyceride 43.8 Squalane 30 Jojoba Oil 24 Glyceryl Behenate 0.7 Palmyra tamarindus bark extract 0.5 Ginger Root Extract 0.5 Saffron Extract 0.5

[0036] Example 6

[0037] The following ingredients were heated to 80° C. and stirred while maintaining the temperature at 80° C. for 1 hour to obtain Composition 6.

[0038]

[0039]

[0040] The beneficial effects of the present invention are further demonstrated by corresponding experimental data below.

[0041] Test experiment 1: Nrf2 antioxidant efficacy test experiment

[0042] The use of in vitro alternative methods has gradually become an important means of evaluating the efficacy, activity and safety of cosmetics. Among them, the 3D imitation skin model can be used as a substitute for human "skin" for basic research and for safety and efficacy evaluation in the cosmetics, chemicals, medical devices, health foods and other industries. The 3D epidermal model EpiKutis uses keratinocytes isolated from Chinese skin tissue as seed cells, and uses a finely adjusted serum-free culture medium to promote the cells to develop into a multi-layered epidermal imitation skin model in vitro. It has a multi-layered structure, physiological and metabolic functions that are highly similar to natural skin, and is highly similar to human skin in histological structure. It contains a stratum corneum, a granular layer, a spinous layer and a basal layer, and is widely used in skin biology research and in vitro efficacy evaluation of cosmetics.

[0043] The specific operation of the method for detecting the content of nuclear transcription factor Nrf2 based on the 3D epidermal skin model is as follows:

[0044] Grouping: the test sample 1 is prepared from Example 1, a blank control group, a negative control group, and a positive control group;

[0045] According to the test grouping, the model was transferred to a 6-well plate (0.9 mL of EpiGrowth culture medium was added in advance), and the test group number was marked on the 6-well plate. 0.9 mL of normal model culture medium was added to each well of the blank control group and the negative control group, and 0.9 mL of model culture medium containing 7 μg / mL vitamin E (VE) was added to each well of the positive control group. VE is a standard agonist of Nrf2 and is often used as an experimental positive control to detect the accuracy of the experimental model. The sample group was treated with the sample to be tested, and the administration method was surface administration. The administration volume was 25 μL / model. When administering, apply it evenly in a gentle circular motion to ensure that the sample is fully absorbed;

[0046] After the administration, all groups except the blank control group were irradiated with UVB at a dose of 600 mJ / cm 2 ;

[0047] After irradiation, the 6-well plate was placed in a CO2,incubator (37°C, 5% CO2) and incubated for 24 h;

[0048] After the incubation, the test substance remaining on the surface of the model was cleaned with sterile PBS, and the residual liquid inside and outside the model was wiped off with a sterile cotton swab;

[0049] The model was removed by ring cutting and fixed with 4% paraformaldehyde. After fixation for 24 hours, immunofluorescence detection of Nrf2 was performed and photos were taken under a microscope for observation.

[0050] Calculation of promotion rate:

[0051] GraphPadPrism was used for drawing, and the results were expressed as Mean±SD. The t-test was used for statistical analysis between the groups. The statistical results were all two-tailed. P<0.05 was considered to be significantly different, marked as *, and P<0.01 was considered to be extremely significantly different, marked as **. The test results are shown in Table 1.

[0052] Table 1 Summary of Nrf2 immunofluorescence analysis results

[0053]

[0054] like Figure 1 , Figure 2 As shown in the figure, the Nrf2 marker in the blank group was obvious, and after the UVB radiation group, the Nrf2 marker part decreased. The Nrf2 marker part of the UVB radiation and smear sample 1 group was significantly increased compared with the UVB radiation group. As shown in Table 1, compared with the UVB radiation group, the Nrf2 content of the UVB radiation and smear sample 1 group was significantly increased by 400.00%. This shows that the combination of the palm bark extract, turmeric root extract and saffron extract can significantly increase the expression of the nuclear transcription factor Nrf2, and has a very significant antioxidant regulatory effect.

[0055] The same test experiments were carried out on the products prepared in Examples 2-6 of the present invention, and the beneficial effects described in the present invention were also achieved.

[0056] Test experiment 2: ROS antioxidant efficacy test experiment

[0057] There are in vitro biochemical methods and cell model methods for free radical scavenging experiments, but these experimental methods are still quite different from the normal physiological state of human skin. The 3D epidermal model EpiKutis is used to detect ROS, which is closer to the skin's own antioxidant state and can truly simulate the antioxidant effect of human skin after using skin care products.

[0058] The specific operation of the reactive oxygen species (ROS) content detection method based on the 3D epidermal skin model is as follows:

[0059] Grouping: the test sample 2 was prepared from Example 4, a blank control group, a negative control group, and a positive control group;

[0060] Dilute the 10mM probe solution to 20uM probe working solution using the model culture medium;

[0061] The model was transferred to a 6-well plate and randomly divided into a blank control group, a negative control group, a positive control group and a sample group, with 3 replicates in each group. 0.9 mL of model culture solution was added to each well of the blank control group and the negative control group, and 0.9 mL of model culture solution containing VE was added to each well of the PC group. VE is a standard inhibitor of ROS and is often used as an experimental positive control to detect the accuracy of the experimental model. The sample group was treated with the corresponding sample, and the sample addition method was surface loading. The sample addition volume was 25uL / model. When adding the sample, try to apply it evenly in a gentle circle to ensure that the sample is fully absorbed.

[0062] After the sample addition, according to the test grouping, except for the blank control group, the rest of the groups were placed under the UVB irradiator (irradiation dose of 600mJ / cm 2 ), after irradiation, the 6-well plate was placed in a CO incubator (37°C, 5% CO ) and incubated for 24 h.

[0063] After the incubation, add 3.7 mL of 20 uM probe working solution to each well and incubate in a CO2 incubator (37°C, 5% CO2) for 1 hour, shaking the well plate every 20 minutes to ensure uniform incubation of the model.

[0064] After the incubation, the model was cut out and placed in a 1.5 mL centrifuge tube. 1 mL of 4% paraformaldehyde fixative was added to the tube, which was then wrapped in tin foil and sent for frozen sectioning.

[0065] The cut frozen sections were washed with PBS for 8 minutes. After washing, the liquid around the tissue was wiped clean with absorbent paper, and a small drop (about 20uL) of fluorescent anti-quenching sealing agent was dropped with a 200u gun tip, and the sections were sealed with a coverslip.

[0066] Observe with a fluorescence microscope and take fluorescence pictures.

[0067] Inhibition rate calculation:

[0068] GraphPadPrism was used for drawing, and the results were expressed as Mean±SD. The t-test was used for statistical analysis between the groups. The statistical results were all two-tailed. P<0.05 was considered to be significantly different, marked as *, and P<0.01 was considered to be extremely significantly different, marked as **. The test results are shown in Table 2.

[0069] Table 2 Summary of ROS integral density (IOD) values

[0070]

[0071] like Figure 3 , Figure 4As shown in the figure, the ROS-marked area in the blank group was less, and after UVB radiation, the ROS-marked area increased significantly. The ROS-marked part of the UVB radiation and smear sample 1 group was significantly reduced compared with the UVB radiation group. As shown in Table 2, compared with the UVB radiation group, the ROS content of the UVB radiation and smear sample 2 group decreased significantly by 50.17%. This shows that the cosmetics containing the extract of the bark of the palm tree, the extract of the turmeric root and the extract of the saffron have a strong ability to inhibit ROS and have a strong antioxidant effect.

[0072] The same test experiments were carried out on the products prepared in Examples 1-3 and 5-6 of the present invention, and the beneficial effects described in the present invention were also achieved.

[0073] Test experiment 3: Melanin inhibition test experiment

[0074] In the past, a large number of whitening experiments used in vitro tyrosinase inhibition methods, which could not reflect the normal process of melanin production in the skin. The 3D melanin skin model uses keratinocytes and melanocytes isolated from Chinese skin tissue as seed cells, and uses finely adjusted serum-free culture medium to promote the cells to develop into a 3D melanin skin model MelaKutis containing a melanocyte stratified structure in vitro. It contains 5%-10% melanocytes, has a highly similar distribution of melanin particles, epidermal stratified structure, physiological and metabolic functions to natural skin, and is highly consistent with human skin. It has been widely used in the field of simulating human whitening efficacy testing.

[0075] The specific operation of the melanin distribution detection method based on the 3D melanin skin model is as follows:

[0076] Grouping: the test sample 3 was prepared from Example 5, a blank control group, a negative control group, and a positive control group;

[0077] Prepare a 6-well plate, add 3.7 mL of melanin model culture solution to each well, and transfer the model on the day of model reception (Day 0) to the marked 6-well plate; each experimental group requires 3 models, and transfer all 6-well plates with models to a CO2 incubator for culture (37°C, 5% CO2);

[0078] From the day the model was received (Day 0), the negative control, positive control, and sample groups were irradiated with UVB (50 mJ / cm 2 ), the blank control group was not irradiated with UVB, and only the culture medium was replaced every day. The positive control group (kojic acid, 500 μg / mL) and the sample group were administered twice on Day 3 and Day 5, respectively, with the administration method being surface administration and the dosage being 2 mg / cm 2Kojic acid is a classic substance that inhibits the production of melanin and is often used as a positive control to test the accuracy of the experimental model. After the model is cultured for 7 consecutive days, samples are collected for testing;

[0079] After fixation with 4% paraformaldehyde solution for 24 h, the sections were embedded and stained according to the instructions of the silver staining kit, and photographed under a microscope;

[0080] Inhibition rate calculation:

[0081] GraphPadPrism was used for drawing, and the results were expressed as Mean±SD. The t-test was used for statistical analysis between the groups. The statistical results were all two-tailed. P<0.05 was considered to be significantly different, marked as *, and P<0.01 was considered to be extremely significantly different, marked as **. The test results are shown in Table 3.

[0082] Table 3 Summary of melanin distribution detection

[0083]

[0084] like Figure 5 , Figure 6 As shown in the figure, the blank group has less melanin marked area, and after UVB radiation, the melanin marked area increased significantly. The melanin marked part of the UVB radiation and smear sample 3 group decreased significantly compared with the UVB radiation group. As shown in Table 3, compared with the UVB radiation group, the melanin content of the UVB radiation and smear sample 3 group decreased significantly by 49.10%. This shows that the cosmetics containing the extract of the bark of the palm tree, the extract of the turmeric root and the extract of the saffron have a strong ability to inhibit the formation of melanin and have a strong whitening effect.

[0085] The same test experiments were carried out on the products prepared in Examples 1-4 and 6 of the present invention, and the beneficial effects described in the present invention were also achieved.

[0086] Test experiment 4: Whitening effect test experiment

[0087] In the whitening efficacy test of skin care products, in addition to measuring the change in the amount of melanin in the skin, a more objective way to reflect the skin whitening effect is to measure the chromaticity and brightness of the skin. Therefore, a 3D melanin skin model that is highly consistent with human skin is used to intuitively analyze the apparent chromaticity and apparent brightness (L* value).

[0088] The specific operation of the apparent chromaticity and apparent brightness (L* value) detection method based on the 3D melanin skin model is as follows:

[0089] Grouping: the test sample 4 was prepared from Example 6, a blank control group, a negative control group, and a positive control group;

[0090] Prepare a 6-well plate, add 3.7 mL of melanin model culture solution to each well, and transfer the model on the day of model reception (Day 0) to the marked 6-well plate; each experimental group requires 3 models, and transfer all 6-well plates with models to a CO2 incubator for culture (37°C, 5% CO2);

[0091] Since the day the model was received (Day 0), the negative control, positive control and sample groups were irradiated with UVB (50 mJ / cm 2 ), the blank control group was not irradiated with UVB, and only the culture medium was replaced every day. The positive control group (kojic acid, 500 μg / mL) and the sample group were administered twice on Day 3 and Day 5, respectively, with the administration method being surface administration and the dosage being 2 mg / cm 2 Kojic acid is a classic ingredient that inhibits melanin production and improves skin brightness. It is often used as a positive control in experiments to test the accuracy of experimental models. After the model is cultured for 7 consecutive days, samples are collected for testing;

[0092] Use a camera to take photos. The specific standard operation of taking photos is as follows: (1) Camera mode: Manual; Photo parameter settings: focal length -5.8mm, aperture = f / 8, aperture F22, shutter speed = 1 / 80S, ISO = 1600. (2) Place the melanin skin model at the center of the colorimetric card and take photos;

[0093] After the apparent colorimetric test, the model is tested for color value. The specific test operation is as follows: Place the model on a flat and hard white surface with the cuticle facing upwards, align the colorimeter detection hole vertically with the model surface for testing, repeat the reading three times for each model and take the average value as the single model's working value reading;

[0094] Calculation of promotion rate:

[0095] GraphPadPrism was used for drawing, and the results were expressed as Mean±SD. The t-test statistical analysis was used for comparison between the groups. The statistical results were all two-tailed. P<0.05 was considered to be significantly different, marked as *, and P<0.01 was considered to be extremely significantly different, marked as **. The test results are shown in Table 4.

[0096] Table 4 Summary of L* value test results

[0097]

[0098] like Figure 7 , Figure 8As shown in Table 4, the L* value of the blank group is biased towards the white area on the left, which is brighter. After UVB radiation, the L* value is biased towards the black area on the right, which is darker. After UVB radiation and application of sample 4, the L* value is more biased towards the white area on the left, which is brighter. As can be seen from Table 4, after UVB radiation and application of sample 4, the L* value is significantly increased by 12.27%. This shows that cosmetics containing palm bark extract, turmeric root extract and saffron extract can effectively improve the L* value and have a good effect of brightening the skin tone.

[0099] The same test experiments were carried out on the products prepared in Examples 1-5 of the present invention, and the beneficial effects described in the present invention were also achieved.

[0100] Comparative Example 1

[0101] Table 5 Nrf2 enhancement rate of a single plant

[0102] Group Nrf2 enhancement rate (%) 0.1% Palmatum bark extract 70.05 0.1% Turmeric root extract 85.88 0.1% Saffron Extract 65.78 Caprylic / capric triglyceride 4.35

[0103] The experimental steps of Comparative Example 1 are the same as those of Test Example 1. As shown in Table 5, the Nrf2 enhancement rates of 0.1% tamarisk bark extract, 0.1% turmeric root extract, and 0.1% saffron extract are 70.05%, 85.88%, and 65.78%, respectively, while the Nrf2 enhancement rate of the combination of tamarisk bark extract, turmeric root extract, and saffron extract is 400%, indicating that tamarisk bark extract, turmeric root extract, and saffron extract have a synergistic effect on Nrf2. In addition, the Nrf2 enhancement rate of caprylic / capric triglyceride is 4%, indicating that the Nrf2 promotion effect of the combination of tamarisk bark extract, turmeric root extract, and saffron extract on Nrf2 is not caused by the solvent, but the efficacy of the composition itself.

[0104] Comparative Example 2

[0105] Table 6 ROS inhibition rate of single plants

[0106]

[0107]

[0108] The experimental steps of ratio 2 are the same as those of test example 2. As shown in Table 6, the ROS inhibition rates of 0.1% tamarisk bark extract, 0.1% turmeric root extract, and 0.1% saffron extract are 27.56%, 30.48%, and 10.64%, respectively, while the inhibition rate of the combination of tamarisk bark extract, turmeric root extract, and saffron extract on ROS is 50.17%, indicating that tamarisk bark extract, turmeric root extract, and saffron extract have a synergistic effect on the inhibition of ROS. In addition, the ROS inhibition rate of 45% caprylic / capric triglyceride + 30% squalane + 24% jojoba oil + 0.7% glyceryl behenate is only 5.75%, indicating that the inhibitory effect of the combination of tamarisk bark extract, turmeric root extract, and saffron extract on ROS is not caused by oil, but the efficacy of the composition itself.

[0109] Comparative Example 3

[0110] Table 7 Melanin inhibition rate of a single plant

[0111]

[0112] The experimental steps of ratio 3 are the same as those of test example 3. As shown in Table 7, the ROS inhibition rates of 0.5% tamarisk bark extract, 0.5% turmeric root extract, and 0.5% saffron extract are 37.75%, 25.46%, and 12.33%, respectively, while the inhibition rate of the combination of tamarisk bark extract, turmeric root extract, and saffron extract on melanin is 49.10%, indicating that tamarisk bark extract, turmeric root extract, and saffron extract have a synergistic effect on the inhibition of melanin. In addition, the melanin inhibition rate of 43.8% caprylic / capric triglyceride + 30% squalane + 24% jojoba oil + 0.7% glyceryl behenate is only 5.65%, indicating that the inhibitory effect of the combination of tamarisk bark extract, turmeric root extract, and saffron extract on melanin is not caused by oil, but the efficacy of the composition itself.

[0113] Comparative Example 4

[0114] Table 8 L* value improvement rate of a single plant

[0115]

[0116] The experimental steps of Comparative Example 4 are the same as those of Test Example 4. As shown in Table 5, the L* value improvement rates of 1.0% tamarisk bark extract, 1.0% turmeric root extract, and 1.0% saffron extract are 3.77%, 5.63%, and 2.96% respectively, while the L* value improvement rate of the combination of tamarisk bark extract, turmeric root extract, and saffron extract is 12.27%, indicating that tamarisk bark extract, turmeric root extract, and saffron extract have a synergistic effect on L* value. In addition, the L* value improvement rate of 42% caprylic / capric triglyceride + 30% squalane + 24% jojoba oil + 1% glyceryl behenate is only 2.22%, indicating that the L* value promotion effect of the combination of tamarisk bark extract, turmeric root extract, and saffron extract is not caused by oil, but the efficacy of the composition itself.

[0117] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plant extract composition having Nrf2 stimulating effect, characterized in that The invention contains the following components in percentage by weight: 0.1-1.0% of ginger root extract, 0.1-1.0% of tamarisk bark extract and 0.1-1.0% of saffron extract.

2. A plant extract composition having Nrf2 stimulating effect as claimed in claim 1 or 2, characterized in that: The ginger root extract contains more than 65% of turmeric ketones, the palm tree bark extract contains more than 80% of pterostilbene, and the saffron extract contains more than 30% of crocetin.

3. A plant extract composition having Nrf2 stimulating effect as claimed in claim 1, characterized in that Also contains: Caprylic / capric triglyceride 42-45%, squalane 30%, jojoba oil 24%, glyceryl behenate 0.7-1%.

4. The method for preparing a plant extract composition having Nrf2 stimulating properties as claimed in claim 2, characterized in that The following steps are involved: Caprylic / capric triglyceride 42-45%, squalane 30%, jojoba oil 24%, glyceryl behenate 0.7-1%, ginger root extract 0.1-1.0%, thuja sabdariffa bark extract 0.1-1.0%, saffron extract 0.1-1.0% are heated to 80°C, and stirred for 1 hour while maintaining the temperature at 60-80°C to obtain a composition.

5. Use of the plant extract composition having Nrf2 stimulating activity as claimed in claim 1 in the preparation of oil cosmetics.

6. Use of the plant extract composition with Nrf2 stimulant as claimed in claim 5 in the preparation of oil cosmetics for antioxidant regulation, strong antioxidant, whitening and brightening skin tone.

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