Application of birch juice separating medium in repairing injured liver cells

Abstract

The invention discloses application of birch juice separation liquid in repairing liver injury cells, and relates to the technical field of medicines.The application comprises the steps that firstly, high-quality birch juice is collected in advance, secondly, the birch juice is purified, thirdly, separation and concentration are conducted, and fourthly, the collected birch juice and the prepared birch juice separation liquid are sterilized and disinfected; the birch juice separation liquid prepared by the method can be used for treating chemical liver injury and repairing liver injury cells, and meanwhile, the birch juice separation liquid can be independently used and can also be combined with medical components with other effects to be used together in a matched mode to treat and repair the liver injury cells and diseases. The birch juice separation liquid can be put into the field of medicine, and can be independently prepared or mixed with other medicaments to prepare one of the forms of tablets, granules and powder; the birch juice separation liquid can be put into the field of life food and can be independently prepared or mixed with other food raw materials to prepare healthy food capable of repairing hepatocytes.

Description

Technical Field

[0001] The invention relates to the field of medical technology, and in particular to application of birch sap separation liquid in repairing liver damaged cells. Background Art

[0002] Birch trees are mainly distributed in Northeast my country, North China, and parts of Southwest China. Natural birch sap is rich in monosaccharides, polysaccharides, proteins, amino acids and other substances, and has good biological effects. Natural birch sap has been put into use in the field of medical technology. Among them, polysaccharides, as the functional ingredient with the highest content in birch sap, can effectively promote glycogen synthesis, enhance the glycogen conversion of monosaccharides such as glucose in the liver, increase the glycogen content, and promote liver function recovery. At the same time, polysaccharides can effectively enhance the resistance of liver cells, nourish and repair damaged liver cells. In addition to the extremely low content of pentacyclic triterpenoid compounds such as betulinic acid and betulin in birch sap, the polysaccharides in birch sap have more efficient and broad use prospects in the treatment of chemical liver damage;

[0003] People who like to drink alcohol are more likely to damage their livers than ordinary people. The probability of liver damage cells in this group of people is 30% higher than that of ordinary people. They need to pay attention to alcohol detoxification and liver protection. The decomposition and metabolism of alcohol in the human body mainly rely on two enzymes in the liver enzyme system, one is alcohol dehydrogenase and the other is acetaldehyde dehydrogenase. Birch sap contains 17 kinds of amino acids, 8 kinds of vitamins, 8 kinds of trace elements, and rich flavonoids, flavanone compounds and polyphenol compounds, including alcohol dehydrogenase and acetaldehyde dehydrogenase required for alcohol detoxification. The two enzymes can decompose alcohol quickly, and the central nervous system will be less stimulated by alcohol and acetaldehyde, reducing the discomfort of the human body after drinking. Moreover, this compound is not artificially synthesized, but is naturally formed by living birch trees absorbing the essence of nature. Birch sap extract, as a natural therapy, is more suitable for the application of birch sap extract in repairing liver damaged cells.

[0004] On this basis, after searching the patent website, Chinese patent application number CN115998783A discloses a birch sap extract that can be used to treat chemical liver damage; the birch sap extract prepared by this patent contains rich polysaccharides, which can effectively improve the glycogen conversion of monosaccharides such as glucose in the liver, promote liver glycogen synthesis, increase liver glycogen content, enhance the resistance of liver cells, protect liver cells, and promote liver function recovery, and can be effectively used for the treatment of chemical liver damage diseases;

[0005] It can be seen that there are shortcomings in the above patent application: the birch juice prepared by the patent contains a large amount of reducing sugar substances such as monosaccharides and disaccharides, which can improve the therapeutic effect of liver damage diseases; however, the reducing sugar therein enters the human body and causes an increase in blood uric acid levels after metabolism by glucokinase, which can increase the probability of gout in the treated patients; and other related nutrients in the birch juice prepared above cannot be absorbed more efficiently by the human body, resulting in a single nutrient available for absorption, affecting the subsequent treatment effect.

[0006] In view of the above problems, an application of birch sap separation in repairing liver damaged cells is proposed. Summary of the invention

[0007] The purpose of the present invention is to provide an application of birch sap separation liquid in repairing liver damaged cells. The device is used to work, thereby solving the problem that the birch sap prepared by the patent contains a large amount of reducing sugar substances such as monosaccharides and disaccharides, which can improve the therapeutic effect of liver damage diseases; however, the reducing sugar enters the human body and causes an increase in blood uric acid levels after metabolism by glucokinase, which can increase the probability of gout in the treated person; and other related nutrients in the birch sap prepared above cannot be absorbed more efficiently by the human body, resulting in a single nutrient available for absorption, affecting the subsequent therapeutic effect.

[0008] To achieve the above object, the present invention provides the following technical solution: a birch sap separation liquid, the preparation process of which adopts an ultrafiltration nanofiltration composite separation and concentration process, and is prepared by the following steps:

[0009] Preferably, step 1: collect high-quality birch sap in advance:

[0010] Raw material selection: Choose high-quality birch trees to ensure the quality of the sap. Choose birch trees: Choose healthy and mature birch trees with a trunk diameter of more than 20 cm. Birch trees with large crowns in the woods are preferred because their sap has better fluidity. Avoid collecting sap in industrial areas, along roadsides, or where the soil has been contaminated;

[0011] Punching: Punch holes on birch trees and determine the punching height: measure the height, the punching height should be between 45cm-65cm to ensure sap collection and birch tree health;

[0012] Insert the medical-grade latex tube into the hole: Use an electric hand drill or awl with a diameter less than 1 cm, make a hole at a 15-degree angle to the bark on the sunny side, and make a hole 3 cm to 5 cm deep. Insert the clean medical-grade latex tube into the hole to form a tight seal;

[0013] Collecting the sap: Use nails or cable ties on both sides of the drilled hole to hang a sterile collection bag on the tree and insert a latex tube into the bag to collect the sap. After a few hours, the birch sap is ready to be harvested.

[0014] Preferably, step 2: purifying birch sap:

[0015] Filtration: Pass the collected juice through a filter to remove impurities;

[0016] Sedimentation: Let the filtered juice stand for a while to allow the impurities to settle to the bottom of the container; Clarification: Carefully scoop out the clarified liquid from the settled juice;

[0017] Boil the clarified birch sap to kill harmful bacteria.

[0018] Preferably, step three: separation and concentration:

[0019] The substances with relative molecular weight in the range of 1.25 kdal to 3.25 kdal in birch sap are separated and concentrated by ultrafiltration and nanofiltration composite separation and concentration process.

[0020] Under the conditions of pressure of 0.9-2.2 MPa, 24-48°C and flow rate of 1.2-4.2 m / s, the natural birch sap stock solution was subjected to primary separation and purification using an ultrafiltration membrane assembly with a molecular weight cutoff of 2000 dal. After the birch sap stock solution was circulated and ultrafiltered for 2.5 hours, the filtrate was collected to obtain a birch sap ultrafiltrate having a relative molecular weight of less than 3.25 dalk.

[0021] The birch sap ultrafiltrate obtained in step 3 (1) is used as a raw material, and the birch sap ultrafiltrate is subjected to secondary concentration separation using a nanofiltration membrane assembly with a molecular weight cutoff of 1.25 kdal at a pressure of 0.9 to 2.2 MPa, 24 to 48° C., and a flow rate of 1.2 to 4.2 m / s. After 2.5 hours of cyclic nanofiltration separation, the filtrate is collected to obtain a birch sap filtrate with a relative molecular weight of 1.25 kdal to 3.25 kdal, and the birch sap ultrafiltrate is filtered through a sterile filter membrane;

[0022] The birch sap ultrafiltrate obtained in step 3 (2) is used as a raw material, and the prepared birch sap nanofiltrate is subjected to three-stage concentration separation using a nanofiltration membrane assembly with a molecular weight cutoff of 1 kdal under the conditions of a pressure of 0.9 to 2.2 MPa, 20 to 45° C., and a flow rate of 1 to 4 m / s. After cyclic nanofiltration separation for 2 hours, a birch sap separated liquid with a relative molecular weight range of 1.25 kdal to 3.25 kdal is obtained, and the birch sap nanofiltrate after filtration is filtered through a sterile filter membrane;

[0023] Preferably, citric acid, sugar substitute, methionine concentrate and water are added to the birch sap ultrafiltrate, wherein the sugar substitute comprises xylitol and L-arabinose, and the birch sap contains 88.8.9-94.5%, citric acid 0.3-0.5%, xylitol: 3-5%, and L-arabinose 6-10%;

[0024] Preferably, a preservative may be added to the birch sap, wherein the preservative contains phenoxyethanol, and the amount of phenoxyethanol added is 0.85 wt %.

[0025] Preferably, the total sugar content of the prepared birch sap extract is not less than 30%, and preferably not less than 45%;

[0026] Preferably, the proportion of polysaccharides in the birch juice extract to the total sugars is not less than 85%, and the proportion of polysaccharides in the total sugars is preferably not less than 88%.

[0027] Preferably, the filtration includes filtration using a 0.18 μm filter membrane, and the sterilizing filter membrane is a 0.16 μm filter membrane.

[0028] Preferably, the collected birch sap and the prepared birch sap separated liquid are sterilized; the birch sap sterilization methods mainly include 90°C heating sterilization and membrane plate filtration technology; when treating contaminated microorganisms in natural birch sap, high temperature heating and membrane plate filtration are more effective means and are the most preferred;

[0029] Preferably, high temperature heating kills microorganisms by increasing the temperature, while membrane filtration removes microorganisms by physical interception. Both methods can effectively ensure the safety and sanitary quality of birch sap.

[0030] The birch sap extract prepared by the method can be used to treat chemical liver damage and repair damaged liver cells. The birch sap extract can be used alone or in combination with other effective medical ingredients to treat chemical liver damage and repair damaged liver cells.

[0031] For application in diseases, birch sap extract can be put into the medical field, and it can be made into tablets, granules, powders, capsules, oral liquids or injections by itself or mixed with other medicines. Birch sap extract can be put into the field of daily food, and it can be made into healthy food that can repair liver cells by itself or mixed with other food raw materials.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] 1. The birch sap separation liquid proposed by the present invention is prepared by a specific process and is rich in polysaccharides. These substances can effectively improve the conversion of glycogen in the liver and promote the synthesis of liver glycogen, thereby enhancing the resistance of liver cells, protecting liver cells, and promoting the recovery of liver function. Birch sap can play an auxiliary role in protecting the liver and promoting bile secretion, and restoring kidney function, so that liver and kidney functions can be strengthened: the strengthening of liver function can increase the ability of the liver to secrete various enzyme preparations; the strengthening of kidney function can increase the permeability of glomeruli and accelerate the elimination of water after alcohol metabolism; and birch sap separation liquid, as a natural therapy, is more suitable for the use of birch sap separation liquid in repairing liver damaged cells.

[0034] 2. As a natural therapy, the preparation process of the birch sap extract of the present invention ensures the effective ingredients and natural characteristics of the birch sap extract, while improving the purity and yield of the product; specifically, the birch sap extract has a repairing effect on liver damaged cells in the following aspects: increasing the liver glycogen content: promoting the glycogen conversion of monosaccharides such as glucose in the liver; enhancing the resistance of liver cells: polysaccharides help protect liver cells and reduce damage; promoting liver function recovery: helping to treat chemical liver damage diseases; the birch sap extract can be used alone or in combination with other functional ingredients for the development of foods and medicines for treating chemical liver damage.

[0035] 3. Through ultrafiltration and nanofiltration composite separation technology, the components in birch sap that are not suitable for gout patients to ingest, such as large molecular proteins, small molecular weight reducing sugars, free fatty acids, etc., are separated, so that the birch sap separated liquid has the characteristics of high polysaccharide content, low reducing sugar, low protein, and low fatty acids. It can not only help patients repair liver cells, but also be suitable for gout patients to ingest. Low reducing sugar enters the human body and causes a small increase in blood uric acid levels after metabolism by glucokinase, which can reduce the incidence rate of gout in the treated patients. In addition, other auxiliary food-grade medical ingredients are added to the birch sap prepared above, which is rich in nutrients for absorption and can be absorbed more efficiently by the human body. The birch sap separated liquid can enhance the subsequent liver cell repair effect on patients. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is a schematic diagram of the process for preparing the birch sap separation solution capable of repairing hepatocytes according to the present invention;

[0037] Figure 2 This is a table showing changes in blood alanine aminotransferase and aspartate aminotransferase activities of each group of mice of the present invention;

[0038] Figure 3 The present invention is a table showing the effect of birch sap extract on the survival rate of rat liver cells. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] In order to further understand the content of the present invention, the present invention is described in detail in conjunction with the accompanying drawings.

[0041] A birch sap separation liquid is prepared by using an ultrafiltration and nanofiltration composite separation and concentration process, and is prepared by the following steps:

[0042] Step 1: Collect high-quality birch sap in advance:

[0043] Raw material selection: Choose high-quality birch trees to ensure the quality of the sap. Choose birch trees: Choose healthy and mature birch trees with a trunk diameter of more than 20 cm. Birch trees with large crowns in the woods are preferred because their sap has better fluidity. Avoid collecting sap in industrial areas, along roadsides, or where the soil has been contaminated;

[0044] Punching: Punch holes on birch trees and determine the punching height: measure the height, the punching height should be between 45cm-65cm to ensure sap collection and birch tree health;

[0045] Insert the medical-grade latex tube into the hole: Use an electric hand drill or awl with a diameter less than 1 cm, make a hole at a 15-degree angle to the bark on the sunny side, and make a hole 3 cm to 5 cm deep. Insert the clean medical-grade latex tube into the hole to form a tight seal;

[0046] Collecting the sap: Use nails or cable ties on both sides of the drilled hole to hang a sterile collection bag on the tree and insert a latex tube into the bag to collect the sap. After a few hours, the birch sap is ready to be harvested.

[0047] Step 2: Purify birch sap:

[0048] Filtration: Pass the collected juice through a filter to remove impurities;

[0049] Sedimentation: Let the filtered juice stand for a while to allow the impurities to settle to the bottom of the container; Clarification: Carefully scoop out the clarified liquid from the settled juice;

[0050] Boil the clarified birch sap to kill harmful bacteria.

[0051] Step 3: Separation and concentration:

[0052] The substances with relative molecular weight in the range of 1.25 kdal to 3.25 kdal in birch sap are separated and concentrated by ultrafiltration and nanofiltration composite separation and concentration process.

[0053] Under the conditions of pressure of 0.9-2.2 MPa, 24-48°C and flow rate of 1.2-4.2 m / s, the natural birch sap stock solution was subjected to primary separation and purification using an ultrafiltration membrane assembly with a molecular weight cutoff of 2000 dal. After the birch sap stock solution was circulated and ultrafiltered for 2.5 hours, the filtrate was collected to obtain a birch sap ultrafiltrate having a relative molecular weight of less than 3.25 dalk.

[0054] The birch sap ultrafiltrate obtained in step 3 (1) is used as a raw material, and the birch sap ultrafiltrate is subjected to secondary concentration separation using a nanofiltration membrane assembly with a molecular weight cutoff of 1.25 kdal at a pressure of 0.9 to 2.2 MPa, 24 to 48° C., and a flow rate of 1.2 to 4.2 m / s. After 2.5 hours of cyclic nanofiltration separation, the filtrate is collected to obtain a birch sap filtrate with a relative molecular weight of 1.25 kdal to 3.25 kdal, and the birch sap ultrafiltrate is filtered through a sterile filter membrane;

[0055] The birch sap ultrafiltrate obtained in step 3 (2) is used as a raw material, and the prepared birch sap nanofiltrate is subjected to three-stage concentration separation using a nanofiltration membrane assembly with a molecular weight cutoff of 1 kdal under the conditions of a pressure of 0.9 to 2.2 MPa, 20 to 45° C., and a flow rate of 1 to 4 m / s. After cyclic nanofiltration separation for 2 hours, a birch sap separated liquid with a relative molecular weight range of 1.25 kdal to 3.25 kdal is obtained, and the birch sap nanofiltrate after filtration is filtered through a sterile filter membrane;

[0056] Adding citric acid, sugar substitute, methionine concentrate and water to birch sap ultrafiltrate, wherein the sugar substitute comprises xylitol and L-arabinose, the birch sap content is 88.8.9-94.5%, the citric acid is 0.3-0.5%, the xylitol is 3-5%, and the L-arabinose is 6-10%;

[0057] A preservative may be added to the birch sap, wherein the preservative contains phenoxyethanol, and the added amount of phenoxyethanol is 0.85 wt %.

[0058] The total sugar content of the prepared birch sap extract is not less than 30%, and preferably not less than 45%;

[0059] The proportion of polysaccharides in the total sugars in the birch sap extract is not less than 85%, and preferably not less than 88%.

[0060] Filtration includes filtration using a 0.18 μm filter membrane, and the sterilizing filter membrane is a 0.16 μm filter membrane.

[0061] The collected birch sap and the prepared birch sap separated liquid are sterilized and disinfected; the birch sap disinfection methods mainly include 90℃ heating sterilization and membrane plate filtration technology; when dealing with contaminated microorganisms in natural birch sap, high temperature heating and membrane plate filtration are more effective means and are the most preferred;

[0062] High temperature heating kills microorganisms by increasing the temperature, while membrane filtration removes microorganisms by physical interception. Both methods can effectively ensure the safety and sanitary quality of birch sap. Example 1: Using birch sap extract to treat chemical liver damage in mice

[0063] Prepare birch sap isolate according to the method of the present invention;

[0064] 100 mice were randomly divided into 5 groups, namely blank group, model group, high-dose, medium-dose and low-dose birch sap extract groups. Except for the blank group, mice in each group were given a one-time intraperitoneal injection of 0.5% CCl4 soybean oil solution 20mL / kg to establish an acute liver injury animal model. The same amount of normal saline solution was injected into the peritoneal cavity of mice in the blank group. After the modeling was completed, mice in the high-dose, medium-dose and low-dose birch sap extract groups were given birch sap gavage at doses of 24ml / kg, 10ml / kg and 2ml / kg, respectively, and mice in the blank group and model group were given an equal volume of normal saline gavage. Each group was intervened for 15 consecutive days. After 15 days, the concentrations of alanine aminotransferase and aspartate aminotransferase in the peripheral blood of mice were measured;

[0065] The experimental results showed that compared with the blank group, the activities of alanine aminotransferase and aspartate aminotransferase in the peripheral blood of mice in the chemical liver injury model group were significantly upregulated. Compared with the model group, the activities of alanine aminotransferase and aspartate aminotransferase in the serum of mice in the high, medium and low dose groups of birch sap extract were significantly reduced and dose-related, that is, the concentration of birch sap extract was inversely proportional to the activities of alanine aminotransferase and aspartate aminotransferase. The experimental results showed that birch sap extract had a good protective effect on liver-damaged cells.

[0066] For details on the changes in blood alanine aminotransferase and aspartate aminotransferase activities of mice in each group, see Figure 1 ;

[0067] Example 2: Experiment on the effect of birch sap extract on rat liver cell survival

[0068] Prepare birch sap isolate according to the method of the present invention;

[0069] Preparation of mouse serum containing birch sap isolate: 30 mice were randomly divided into 3 groups and gavaged with birch sap isolate at doses of 24 ml / kg, 10 ml / kg and 2 ml / kg respectively for 25 consecutive days, twice a day. Femoral artery blood was collected every day and serum was separated and collected. The sera in the same group were mixed to prepare mouse sera containing high, medium and low birch sap isolate contents, which were then stored in a refrigerator for later use.

[0070] Cell culture and grouping: BRL cells in the logarithmic growth phase were divided into 4 groups, namely a blank control group, and birch sap (high, medium, and low birch sap extract serum content) groups.

[0071] Cells in the logarithmic growth phase were prepared into cell suspensions with cell culture medium. On this basis, 30 μl of serum containing different birch sap isolate contents were added to the high, medium and low dose groups of birch sap isolate, and a blank control group was set up. Cells in the logarithmic growth phase of each group were placed in a 48-well plate, cultured for 72 hours, and then the supernatant was added and cultured for another 6 hours, and the supernatant was discarded to calculate the cell survival rate.

[0072] The experimental results showed that compared with the blank control group, the cell survival rate of the model group was significantly reduced; compared with the model group, the cell survival rates of each dose group of birch sap extract were significantly improved and dose-related, indicating that birch sap extract has a good protective effect on chemically damaged liver cells.

[0073] For details on the effect of birch sap extract on the survival rate of rat hepatocytes, see Figure 2 .

[0074] It should be noted that the above-mentioned electrical components are equipped with power supplies and their control methods are prior art. In order to avoid redundant description, they are uniformly described here; and this application is mainly used to protect mechanical equipment, so the control method and circuit connection are no longer explained in detail. In this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0075] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A birch sap separation liquid, characterized in that: The birch sap separation liquid is prepared by using an ultrafiltration nanofiltration composite separation and concentration process, and is prepared by the following steps: Step 1: Collect high-quality birch sap in advance: (1) Raw material selection: Select high-quality birch trees to ensure the quality of the sap. Select birch trees: Select healthy and mature birch trees with a trunk diameter of 20 cm. Birch trees with larger crowns in the forest are preferred because their sap has better fluidity. Avoid collecting sap in industrial areas, along roadsides, or where the soil has been contaminated; (2) Punching: Punch holes in birch trees and determine the drilling height: measure the height. The drilling height should be between 45cm and 65cm to ensure sap collection and birch tree health. (3) Insert the medical-grade latex tube into the hole: Use an electric hand drill or awl with a diameter of less than 1 cm, make a hole at a 15-degree angle to the bark on the sunny side, and make a hole 3 cm to 5 cm deep. Insert a clean medical-grade latex tube into the hole to form a tight seal; (4) Collecting sap: Use nails or cable ties on both sides of the drilled hole to hang a sterile collection bag on the tree and insert a latex tube into the bag to collect the sap. After a few hours, the birch sap can be harvested. Step 2: Purify birch sap: (1) Filtration: The collected juice is passed through a filter to remove impurities; (2) Sedimentation: Let the filtered juice stand for a while to allow the impurities to settle to the bottom of the container; Clarification: Carefully scoop out the clarified liquid from the settled juice; (3) Boil the clarified birch sap to kill harmful bacteria. Step 3: Separation and concentration: The substances with relative molecular weight in the range of 1.25 kdal to 3.25 kdal in birch sap are separated and concentrated by ultrafiltration and nanofiltration composite separation and concentration process. (1) Under the conditions of pressure of 0.9-2.2 MPa, 24-48° C., and flow rate of 1.2-4.2 m / s, a natural birch sap stock solution was subjected to primary separation and purification using an ultrafiltration membrane assembly with a molecular weight cutoff of 2000 dal. After the birch sap stock solution was circulated and ultrafiltered for 2.5 hours, the filtrate was collected to obtain a birch sap ultrafiltrate having a relative molecular weight of less than 3.25 dal. (2) Using the birch sap ultrafiltrate obtained in step 3 (1) as a raw material, the birch sap ultrafiltrate is subjected to secondary concentration separation using a nanofiltration membrane assembly with a molecular weight cutoff of 1.25 kdal at a pressure of 0.9 to 2.2 MPa, 24 to 48° C., and a flow rate of 1.2 to 4.2 m / s. After circulating the nanofiltration separation for 2.5 hours, the filtrate is collected to obtain a birch sap filtrate with a relative molecular weight of 1.25 kdal to 3.25 kdal, and the birch sap ultrafiltrate is filtered through a sterile filter membrane; (3) Using the birch sap ultrafiltrate obtained in step 3 (2) as a raw material, the prepared birch sap nanofiltrate is subjected to three-stage concentration separation using a nanofiltration membrane assembly with a molecular weight cutoff of 1 kdal under the conditions of a pressure of 0.9 to 2.2 MPa, 20 to 45° C., and a flow rate of 1 to 4 m / s. After cyclic nanofiltration separation for 2 hours, a birch sap separated liquid having a relative molecular weight range of 1.25 kdal to 3.25 kdal is obtained, and the birch sap nanofiltrate after filtration is filtered through a sterile filter membrane; (4) adding citric acid, sugar substitute, methionine concentrate and water to the birch sap ultrafiltrate, wherein the sugar substitute comprises xylitol and L-arabinose, and the birch sap contains 88.8.9-94.5%, citric acid 0.3-0.5%, xylitol: 3-5%, and L-arabinose 6-10%; (5) A preservative may be added to the birch sap, wherein the preservative contains phenoxyethanol, and the amount of phenoxyethanol added is 0.85 wt %.

2. The birch sap extract according to claim 1, characterized in that: The total sugar content of the prepared birch sap separated liquid is not less than 30%, and preferably not less than 45%.

3. The birch sap extract according to claim 1, characterized in that: The proportion of polysaccharides in the total sugars in the birch sap extract is not less than 85%, and preferably not less than 88%.

4. The birch sap extract according to claim 1, characterized in that: The filtration includes filtration using a 0.18 μm filter membrane, and the sterilizing filter membrane is a 0.16 μm filter membrane.

5. The birch sap extract according to claim 1, characterized in that: The collected birch sap and the prepared birch sap separated liquid are sterilized and disinfected; the birch sap disinfection methods mainly include 90°C heating sterilization and membrane plate filtration technology; when treating contaminated microorganisms in natural birch sap, high temperature heating and membrane plate filtration are more effective means and are the most preferred; High-temperature heating kills microorganisms by increasing the temperature, while membrane filtration removes microorganisms by physical interception. Both methods can effectively ensure the safety and sanitary quality of birch sap.

6. The birch sap extract according to claim 5, characterized in that: The birch sap extract prepared by the method can be used to treat chemical liver damage and repair damaged liver cells. The birch sap extract can be used alone or in combination with other effective medical ingredients to treat chemical liver damage and repair damaged liver cells.

7. The birch sap extract according to claim 6, characterized in that: For application in diseases, birch sap extract can be put into the medical field, and it can be made into tablets, granules, powders, capsules, oral liquids or injections by itself or mixed with other medicines. Birch sap extract can be put into the field of daily food, and it can be made into healthy food that can repair liver cells by itself or mixed with other food raw materials.

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