Chicken anti-stress immunopotentiator in high-temperature and high-humidity state as well as preparation method and application thereof

Abstract

The invention discloses a chicken anti-stress immunopotentiator in a high-temperature and high-humidity state. The chicken anti-stress immunopotentiator is prepared from the following raw materials in parts by weight: 40-55 parts of pine needles, 35-45 parts of herba cynomorii, 30-35 parts of yucca, 25-35 parts of watermelon peel, 20-30 parts of banana peel, 15-25 parts of rhizoma belamcandae, 15-23 parts of pitaya peel, 10-15 parts of cortex acanthopanacis semticosi and 5-10 parts of folium mori. The invention further discloses a preparation method of the chicken anti-stress immunopotentiator in the high-temperature and high-humidity state and application of the chicken anti-stress immunopotentiator in the high-temperature and high-humidity state to prevention and treatment of chicken stress reaction in the high-temperature and high-humidity environment. The chicken anti-stress immunopotentiator in the high-temperature and high-humidity state can quickly act on intestinal tracts of chickens to relieve discomfort caused by the high-temperature and high-humidity state, so that the anti-stress effect is achieved, and feed mildew can be prevented by adding the chicken anti-stress immunopotentiator into feed; and pathogenic microorganisms in water can be effectively restrained by adding the compound into drinking water.

Description

Technical Field

[0001] The invention belongs to the technical field of poultry breeding, and in particular relates to an anti-stress immunity enhancer for chickens under high temperature and high humidity conditions, and a preparation method and application thereof. Background Art

[0002] Chickens are birds with a special physiological structure. They do not have sweat glands and cannot regulate their body temperature by sweating in hot seasons. Therefore, chickens are particularly stressed in high temperature environments, which seriously reduces their production performance. If the air humidity is high in hot seasons, the adverse stress response of chickens will be aggravated. The main manifestations are: in a high temperature and high humidity environment, the heat dissipation capacity of the chicken's body is reduced, resulting in an increase in body temperature, which in turn affects the ovarian function and reduces the egg production rate of laying hens; the high temperature and high humidity environment will also increase the ammonia concentration in the air in the chicken house, which has a stimulating effect on the respiratory and digestive tracts of chickens, thereby affecting the growth and development of chickens; the high humidity environment is a hotbed for the breeding of parasites, which accelerates the hatching speed of parasites and increases the chance of chickens being infected with parasites; the high temperature and high humidity chicken house is prone to breeding bacteria and viruses, which will lead to a decrease in the immunity and resistance of the chickens in the long run, thereby increasing the risk of chickens contracting diseases; the high temperature and high humidity environment will increase the perceived temperature of the chickens, which is easy to cause wind-heat colds in chickens and induce intestinal diseases; in a high temperature and high humidity environment, the feed is prone to mildew, destroying the feed ingredients, reducing the feed intake of the breeder chickens and reducing the egg production rate. At the same time, Aspergillus poisoning will occur after eating moldy feed; the high temperature and high humidity environment will increase the possibility of contamination of the breeding eggs. After the bacteria enter the eggs, they may cause dead embryos, weak embryos or weak chicks during the incubation process, and the mortality rate is high in the early stage of brooding.

[0003] At present, the main means to deal with the problem of high temperature and high humidity environment causing adverse stress to chickens is to improve the cooling system of the chicken house, such as using water curtain fans for longitudinal ventilation, reducing the room temperature of the chicken house as much as possible, or adding anti-stress electrolyte multivitamins to the drinking water. However, in actual production, these measures have little effect. The chickens still have many adverse stress reactions caused by the high temperature and high humidity environment, such as reduced or even no diet, severely reduced production performance, death from heat stroke, and death from other diseases. If the chickens are sick, they need to purchase corresponding drugs for treatment, which increases the cost of treatment. Whether it is laying hens or broilers, if effective measures are not taken in a high temperature and high humidity environment, the quality of eggs and broilers will be seriously reduced.

[0004] Therefore, there is an urgent need to develop an anti-stress immunity enhancer for chickens under high temperature and high humidity conditions that can effectively reduce the adverse stress response caused by the high temperature and high humidity environment to chickens. Summary of the invention

[0005] An object of the present invention is to solve at least the above-mentioned drawbacks and to provide advantages which will be described hereinafter.

[0006] Another object of the present invention is to provide a chicken anti-stress immunity enhancer capable of preventing feed from mildewing under high temperature and high humidity conditions.

[0007] Another object of the present invention is to provide a chicken anti-stress immunity enhancer that can effectively contain pathogenic microorganisms in water under high temperature and high humidity conditions.

[0008] Another object of the present invention is to provide a chicken anti-stress immunity enhancer under high temperature and high humidity conditions that can improve egg production rate and egg quality.

[0009] Another object of the present invention is to provide a chicken anti-stress immunity enhancer under high temperature and high humidity conditions that can improve sperm quality.

[0010] In order to achieve these purposes and other advantages of the present invention, an anti-stress immunity enhancer for chickens under high temperature and high humidity conditions is provided, comprising the following raw materials in parts by weight: 40-55 parts of pine needles, 35-45 parts of cynomorium songaricum, 30-35 parts of yucca, 25-35 parts of watermelon peel, 20-30 parts of banana peel, 15-25 parts of belamcanda chinensis, 15-23 parts of pitaya peel, 10-15 parts of acanthopanax senticosus peel, and 5-10 parts of mulberry leaves.

[0011] In the above scheme, firstly, pine needles, Cynomorium songaricum, yucca, watermelon peel, banana peel, Belamcanda chinensis, pitaya peel, Acanthopanax senticosus peel and mulberry leaves are used as main ingredients, which can not only achieve the effect of disinfection and sterilization, but also the combination of each component can quickly act on the intestines of chickens to relieve the discomfort caused by high temperature and high humidity, thereby achieving an anti-stress effect.

[0012] Secondly, the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention has an antibacterial effect itself, and can prevent the feed from mildewing when added to the feed.

[0013] In addition, the chicken anti-stress immunity enhancer of the present invention has an antibacterial effect under high temperature and high humidity conditions. When added to drinking water, it can effectively curb pathogenic microorganisms in the water, improve the water quality of the used water, and reduce the probability of chickens getting sick due to drinking unclean water sources.

[0014] The method for preparing a chicken anti-stress immune enhancer under high temperature and high humidity conditions comprises: The following raw materials in parts by weight are obtained: 40-55 parts of pine needles, 35-45 parts of cynomorium songaricum, 30-35 parts of yucca, 25-35 parts of watermelon peel, 20-30 parts of banana peel, 15-25 parts of belamcanda chinensis, 15-23 parts of pitaya peel, 10-15 parts of acanthopanax senticosus peel, and 5-10 parts of mulberry leaves.

[0015] The raw materials are washed, dried, and crushed in sequence, and then added with boiling water and stirred into a paste, which is then frozen at -25°C for at least 24 hours and thawed at room temperature to obtain a freeze-thawed material.

[0016] The freeze-thawed material was subjected to organic extraction and vacuum concentration in sequence to obtain a clear paste with a density of 1.18-1.25.

[0017] The clear paste and the organic acid are mixed in a mass ratio of 1:16.7 to obtain the chicken anti-stress immunity enhancer under high temperature and high humidity conditions.

[0018] In the above scheme, specifically, the particle size of the raw material after crushing is 50 mesh, and the ambient temperature of other operations except freezing treatment is 18-30°C, and the relative humidity is 30%-70%. The raw materials are processed in proportion to prepare the chicken anti-stress immune enhancer under high temperature and high humidity conditions of the present invention. The preparation method is simple and easy to operate, and is suitable not only for small-scale breeding farms but also for large-scale breeding bases.

[0019] Secondly, because the chicken anti-stress immune enhancer is first subjected to freeze-thaw treatment under high temperature and high humidity conditions, the raw material components are effectively physically cracked, and then combined with organic extraction to further quickly dissolve the beneficial ingredients of the components, the preparation efficiency is high, and it is convenient to prepare and use it in a timely manner according to actual needs during the production process.

[0020] Preferably, the organic extraction specifically comprises: mixing the freeze-thawed material with a 95% ethanol solution in a volume ratio of 1:3, extracting at room temperature for 3 hours, repeating the filtration at least 3 times, combining the filtrates and removing the ethanol by vacuum distillation.

[0021] Preferably, the vacuum concentration specifically comprises: placing the organically extracted extract in a vacuum rotary apparatus in a water bath at a constant temperature of 60° C. and concentrating the extract until the relative density reaches 1.18-1.25.

[0022] Preferably, the organic acid comprises a mixture of methionine, taurine, formic acid, lactic acid and citric acid in a mass ratio of 1:1.2:1.5:0.6:0.3.

[0023] In the above scheme, by mixing methionine, taurine, formic acid, lactic acid and citric acid in a certain proportion with the clear paste, the prepared chicken anti-stress immune enhancer can quickly adjust the pH value inside the chicken intestine under high temperature and high humidity conditions, so that the chicken intestine is in an acidic environment, helping the chicken to digest quickly to stimulate appetite and increase feed intake, while reducing the reproduction of harmful bacteria in the intestine.

[0024] The chicken anti-stress immunity enhancer under high temperature and high humidity conditions is used for preventing and treating chicken stress reactions under high temperature and high humidity conditions.

[0025] Preferably, for chickens aged 1 to 2 weeks, the mixture is added at 500 ppm and mixed with chicken feed for feeding.

[0026] For chickens aged 3 to 4 weeks, add 200 ppm to chicken feed and feed.

[0027] For chickens over 5 weeks old, add 100 ppm to chicken feed.

[0028] In the above scheme, the amount of chicken anti-stress immune enhancer added to the feed under high temperature and high humidity conditions is adjusted according to the age of the chickens, so as to avoid adverse effects on the chickens caused by excessive addition under the premise of alleviating the discomfort caused by high temperature and high humidity conditions and achieving the anti-stress effect.

[0029] Preferably, for chickens aged 1 to 2 weeks, 2500 ppm is added to drinking water and added twice a week, each time for 8 hours; for chickens aged 3 to 4 weeks, 1500 ppm is added to drinking water and added twice a week, each time for 8 hours; for chickens aged 5 weeks and above, 500 ppm is added to drinking water and added twice a week, each time for 8 hours.

[0030] Preferably, for chickens aged 1 to 2 weeks, 700 ppm is added to drinking water, added once a day, and each time is drunk for 8 hours; for chickens aged 3 to 4 weeks, 400 ppm is added to drinking water, added once a day, and each time is drunk for 8 hours; for chickens aged 5 weeks and above, 150 ppm is added to drinking water, added once a day, and each time is drunk for 8 hours.

[0031] In the above scheme, the amount of chicken anti-stress immune enhancer added to drinking water under high temperature and high humidity conditions is adjusted according to the age of the chickens, so as to effectively curb pathogenic microorganisms in the water while alleviating the discomfort caused by high temperature and high humidity conditions to achieve an anti-stress effect.

[0032] Advantages of the present invention: Firstly, the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention has pine needles, Cynomorium songaricum, yucca, watermelon peel, banana peel, Belamcanda chinensis, pitaya peel, Acanthopanax senticosus peel and mulberry leaves as main ingredients, which can not only achieve the effect of disinfection and sterilization, but also the combination of each component can quickly act on the intestines of chickens to relieve the discomfort caused by high temperature and high humidity conditions, thereby achieving the anti-stress effect.

[0033] Secondly, the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention has an antibacterial effect itself, and can prevent the feed from mildewing when added to the feed.

[0034] In addition, the chicken anti-stress immunity enhancer of the present invention has an antibacterial effect under high temperature and high humidity conditions. When added to drinking water, it can effectively curb pathogenic microorganisms in the water, improve the water quality of the used water, and reduce the probability of chickens getting sick due to drinking unclean water sources.

[0035] In addition, the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention is prepared by processing the raw materials in proportion to obtain the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention. The preparation method is simple and easy to operate, and is not only suitable for small-scale breeding farms but also for large-scale breeding bases.

[0036] In addition, the chicken anti-stress immune enhancer of the present invention is first subjected to freeze-thaw treatment under high temperature and high humidity conditions, thereby effectively physically cracking the raw material components, and then combined with organic extraction to further quickly dissolve the beneficial ingredients of the components. The preparation efficiency is high, and it is convenient to prepare and use it in time according to actual needs during the production process.

[0037] In addition, the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention is prepared by mixing methionine, taurine, formic acid, lactic acid, and citric acid with clear paste in a certain proportion, so that the prepared chicken anti-stress immunity enhancer under high temperature and high humidity conditions can quickly adjust the pH value inside the chicken intestine, so that the chicken intestine is in an acidic environment, helping the chicken to digest quickly to stimulate appetite and increase feed intake, while reducing the reproduction of harmful bacteria in the intestine. DETAILED DESCRIPTION

[0038] The present invention is further described in detail below in conjunction with embodiments so that those skilled in the art can implement the invention with reference to the description.

[0039] It should be understood that terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0040] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial channels unless otherwise specified.

[0041] In the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "set" should be understood in a broad sense. For example, it can be fixedly connected or set, or it can be detachably connected or set, or connected or set in one piece. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The terms "lateral", "longitudinal", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. indicate directions or positional relationships, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present invention.

[0042] The chicken anti-stress immunity enhancer under high temperature and high humidity conditions comprises the following raw materials in parts by weight: 40-55 parts of pine needles, 35-45 parts of cynomorium songaricum, 30-35 parts of yucca, 25-35 parts of watermelon peel, 20-30 parts of banana peel, 15-25 parts of belamcanda chinensis, 15-23 parts of pitaya peel, 10-15 parts of acanthopanax senticosus peel, and 5-10 parts of mulberry leaves.

[0043] The method for preparing a chicken anti-stress immune enhancer under high temperature and high humidity conditions comprises: The following raw materials in parts by weight are obtained: 40-55 parts of pine needles, 35-45 parts of cynomorium songaricum, 30-35 parts of yucca, 25-35 parts of watermelon peel, 20-30 parts of banana peel, 15-25 parts of belamcanda chinensis, 15-23 parts of pitaya peel, 10-15 parts of acanthopanax senticosus peel, and 5-10 parts of mulberry leaves.

[0044] The raw materials are washed, dried, and crushed in sequence, and then added with boiling water and stirred into a paste, which is then frozen at -25°C for at least 24 hours and thawed at room temperature to obtain a freeze-thawed material.

[0045] The freeze-thawed material was subjected to organic extraction and vacuum concentration in sequence to obtain a clear paste with a density of 1.18-1.25.

[0046] The clear paste and the organic acid are mixed in a mass ratio of 1:16.7 to obtain the chicken anti-stress immunity enhancer under high temperature and high humidity conditions.

[0047] Furthermore, the ambient temperature for other operations except the freezing process is 18-30° C., and the relative humidity is 30%-70%.

[0048] Furthermore, the particle size of the raw material after crushing is 50 mesh.

[0049] Furthermore, the organic extraction specifically includes: mixing the freeze-thawed material with a 95% ethanol solution in a volume ratio of 1:3, extracting at room temperature for 3 hours, repeating the filtration at least 3 times, and combining the filtrates to evaporate the ethanol through reduced pressure distillation.

[0050] Furthermore, the vacuum concentration specifically includes: placing the organically extracted extract in a vacuum rotary apparatus in a water bath at a constant temperature of 60° C. and concentrating the extract until the relative density reaches 1.18-1.25.

[0051] Furthermore, the organic acid is obtained by mixing methionine, taurine, formic acid, lactic acid and citric acid in a mass ratio of 1:1.2:1.5:0.6:0.3.

[0052] Example 1 Take 40 parts of pine needles, 35 parts of Cynomorium songaricum, 30 parts of yucca, 25 parts of watermelon peel, 30 parts of banana peel, 15 parts of Belamcanda chinensis, 23 parts of pitaya peel, 15 parts of Acanthopanax senticosus peel and 5 parts of mulberry leaves respectively; wash, dry and crush them in sequence, add boiling water and stir into a paste, then freeze at -25°C for 24 hours, transfer to 25°C for thawing to obtain a freeze-thawed product; mix the freeze-thawed product with 95% ethanol solution in a volume ratio of 1:3, extract at room temperature for 3 hours, repeat the filtration at least 3 times, combine the filtrate, evaporate the ethanol by reduced pressure distillation, and then place it in a vacuum rotary instrument in a water bath at a constant temperature of 60°C to concentrate it to a relative density of 1.18-1.25 to obtain a clear paste; mix the clear paste with an organic acid in a mass ratio of 1:16.7 to obtain the chicken anti-stress immune enhancer under high temperature and high humidity conditions. Among them, the ambient temperature of other operations except freezing treatment is 18-30°C, and the relative humidity is 30%-70%; the particle size of the raw materials after crushing is 50 mesh; the amount of chicken anti-stress immune enhancer prepared each time under high temperature and high humidity conditions is based on the feeding amount for 7-10 days, and the relative density of the clear paste reaches the range of 1.18-1.25; the organic acid is a mixture of methionine, taurine, formic acid, lactic acid and citric acid in a mass ratio of 1:1.2:1.5:0.6:0.3.

[0053] Example 2 Take 55 parts of pine needles, 45 parts of Cynomorium songaricum, 35 parts of yucca, 35 parts of watermelon peel, 20 parts of banana peel, 25 parts of Belamcanda chinensis, 15 parts of pitaya peel, 10 parts of Acanthopanax senticosus peel and 10 parts of mulberry leaves respectively; wash, dry and crush them in sequence, add boiling water and stir into a paste, then freeze at -25°C for 24 hours, transfer to 25°C for thawing to obtain a freeze-thawed product; mix the freeze-thawed product with 95% ethanol solution in a volume ratio of 1:3, extract for 3 hours at room temperature, repeat the filtration at least 3 times, combine the filtrate, evaporate the ethanol by reduced pressure distillation, and then place it in a vacuum rotary instrument in a water bath at a constant temperature of 60°C to concentrate it to a relative density of 1.18-1.25 to obtain a clear paste; mix the clear paste with an organic acid in a mass ratio of 1:16.7 to obtain the chicken anti-stress immune enhancer under high temperature and high humidity conditions. Among them, the ambient temperature of other operations except freezing treatment is 18-30°C, and the relative humidity is 30%-70%; the particle size of the raw materials after crushing is 50 mesh; the amount of chicken anti-stress immune enhancer prepared each time under high temperature and high humidity conditions is based on the feeding amount for 7-10 days, and the relative density of the clear paste reaches the range of 1.18-1.25; the organic acid is a mixture of methionine, taurine, formic acid, lactic acid and citric acid in a mass ratio of 1:1.2:1.5:0.6:0.3.

[0054] Example 3 Take 55 parts of pine needles, 45 parts of Cynomorium songaricum, 35 parts of yucca, 35 parts of watermelon peel, 20 parts of banana peel, 25 parts of Belamcanda chinensis, 15 parts of pitaya peel, 10 parts of Acanthopanax senticosus peel and 10 parts of mulberry leaves respectively; wash, dry and crush them in sequence, add boiling water and stir into a paste, then freeze at -25°C for 24 hours, transfer to 25°C for thawing to obtain a freeze-thawed product; mix the freeze-thawed product with 95% ethanol solution in a volume ratio of 1:3, extract for 3 hours at room temperature, repeat the filtration at least 3 times, combine the filtrate, evaporate the ethanol by reduced pressure distillation, and then place it in a vacuum rotary instrument in a water bath at a constant temperature of 60°C to concentrate it to a relative density of 1.18-1.25 to obtain a clear paste; mix the clear paste with an organic acid in a mass ratio of 1:16.7 to obtain the chicken anti-stress immune enhancer under high temperature and high humidity conditions. Among them, the ambient temperature of other operations except freezing treatment is 18~30℃, and the relative humidity is 30%~70%; the particle size of the raw material after crushing is 50 mesh; the amount of chicken anti-stress immune enhancer prepared each time under high temperature and high humidity conditions is based on the feeding amount for 7~10 days, and the relative density of the clear paste can reach the range of 1.18~1.25; the organic acid is formic acid.

[0055] Example 4 Take 55 parts of pine needles, 45 parts of Cynomorium songaricum, 35 parts of yucca, 35 parts of watermelon peel, 20 parts of banana peel, 25 parts of Belamcanda chinensis, 15 parts of pitaya peel, 10 parts of Acanthopanax senticosus peel and 10 parts of mulberry leaves respectively; wash, dry and crush them in sequence, add boiling water and stir into a paste, then freeze at -25°C for 24 hours, transfer to 25°C for thawing to obtain a freeze-thawed product; mix the freeze-thawed product with 95% ethanol solution in a volume ratio of 1:3, extract for 3 hours at room temperature, repeat the filtration at least 3 times, combine the filtrate, evaporate the ethanol by reduced pressure distillation, and then place it in a vacuum rotary instrument in a water bath at a constant temperature of 60°C to concentrate it to a relative density of 1.18-1.25 to obtain a clear paste; mix the clear paste with an organic acid in a mass ratio of 1:16.7 to obtain the chicken anti-stress immune enhancer under high temperature and high humidity conditions. Among them, the ambient temperature of other operations except freezing treatment is 18~30℃, and the relative humidity is 30%~70%; the particle size of the raw material after crushing is 50 mesh; the amount of chicken anti-stress immune enhancer prepared each time under high temperature and high humidity conditions is based on the feeding amount for 7~10 days, and the relative density of the clear paste reaches the range of 1.18~1.25; the organic acid is lactic acid.

[0056] Example 5 Take 55 parts of pine needles, 45 parts of Cynomorium songaricum, 35 parts of yucca, 35 parts of watermelon peel, 20 parts of banana peel, 25 parts of Belamcanda chinensis, 15 parts of pitaya peel, 10 parts of Acanthopanax senticosus peel and 10 parts of mulberry leaves respectively; wash, dry and crush them in sequence, add boiling water and stir into a paste, then freeze at -25°C for 24 hours, transfer to 25°C for thawing to obtain a freeze-thawed product; mix the freeze-thawed product with 95% ethanol solution in a volume ratio of 1:3, extract for 3 hours at room temperature, repeat the filtration at least 3 times, combine the filtrate, evaporate the ethanol by reduced pressure distillation, and then place it in a vacuum rotary instrument in a water bath at a constant temperature of 60°C to concentrate it to a relative density of 1.18-1.25 to obtain a clear paste; mix the clear paste with an organic acid in a mass ratio of 1:16.7 to obtain the chicken anti-stress immune enhancer under high temperature and high humidity conditions. Among them, the ambient temperature of other operations except freezing treatment is 18-30°C, and the relative humidity is 30%-70%; the particle size of the raw material after crushing is 50 mesh; the amount of chicken anti-stress immune enhancer prepared each time under high temperature and high humidity conditions is based on the feeding amount for 7-10 days, and the relative density of the clear paste can reach the range of 1.18-1.25; the organic acid is a mixture of lactic acid and citric acid with a mass ratio of 2:1.

[0057] Comparative Example 1 Take 55 parts of pine needles, 45 parts of Cynomorium songaricum, 35 parts of yucca, 35 parts of watermelon peel, 20 parts of banana peel, 25 parts of Belamcanda chinensis, 15 parts of pitaya peel, 10 parts of Acanthopanax senticosus peel and 10 parts of mulberry leaves respectively; wash, dry and crush them in sequence, add boiling water and stir into a paste, then freeze at -25°C for 24 hours, transfer to 25°C for thawing to obtain a freeze-thawed product; mix the freeze-thawed product with 95% ethanol solution in a volume ratio of 1:3, extract at room temperature for 3 hours, repeat the filtration at least 3 times, combine the filtrates, evaporate the ethanol through reduced pressure distillation, and then place them in a vacuum rotary instrument in a water bath at a constant temperature of 60°C to concentrate to a relative density of 1.18-1.25, so as to obtain the chicken anti-stress immune enhancer under high temperature and high humidity conditions. Among them, the ambient temperature for other operations except freezing treatment is 18~30℃, and the relative humidity is 30%~70%; the amount of chicken anti-stress immune enhancer prepared under high temperature and high humidity conditions each time is based on the feeding amount for 7~10 days, and the relative density of the clear paste can reach the range of 1.18~1.25.

[0058] Comparative Example 2 32 parts of pine needles, 28 parts of astragalus, 23 parts of grapefruit peel, 23 parts of cinnamon, 15 parts of isatis indigo leaf, 15 parts of ophiopogon and 12 parts of radix rehmanniae are taken respectively; they are crushed into powder to obtain Chinese medicine powder; the Chinese medicine powder is mixed with 90-100°C distilled water, stirred into a paste, and then frozen at -25°C for 20-30 hours, and then thawed at room temperature to obtain a freeze-thawed product; the freeze-thawed product is mixed with brown sugar in a mass ratio of 2-3:5 and placed in a sealed container, and naturally fermented at 15-20°C for 3-5 days to obtain a first fermentation liquid and a first fermentation residue, and the first fermentation residue is ground to obtain a paste; the paste is mixed with bacillus and yeast in a mass ratio of 100:0.1:0.1, and fermented at 30-35°C for 30-40 hours to obtain a second fermentation liquid; the first fermentation liquid, the second fermentation liquid and the prebiotics are dried at 100-105°C to obtain a Chinese medicine composition. The mass ratio of the first fermentation liquid to the prebiotics is 0.1-0.2:100.

[0059] Test and analysis Test 1: The test site is a biotechnology company in Gaoyou City, Yangzhou City, Jiangsu Province, and the test subjects are laying hen feed and broiler feed. The test time is from July 3, 2024 to July 10, 2024. During the test, the average temperature at the test site is 30.2℃ and the average humidity is 87.7%. Each chicken feed is 8 kg, and each feed is divided into 8 groups on average, with 1 kg of feed in each group; among them, test groups 1 to test groups 5 respectively use the chicken anti-stress immune enhancer prepared in high temperature and high humidity conditions in Examples 1 to 5 and add it to the feed at an addition amount of 100 ppm; control group 1 uses the chicken anti-stress immune enhancer prepared in high temperature and high humidity conditions in comparative example 1 and adds it to the feed at an addition amount of 100 ppm; control group 2 uses the Chinese medicine composition prepared in comparative example 2 and adds it to the feed at an addition amount of 100 ppm; the blank group does not add. The feed of each group was left open in the air, and the feed conditions were observed every day. The results are shown in Table 1.

[0060] Table 1: Feed situation of each group According to the data in Table 1, the chicken anti-stress immunity enhancer of the present invention mixed with feed under high temperature and high humidity conditions can effectively prevent the feed from becoming moldy, thereby preventing the problem of chickens inducing diseases due to eating moldy feed.

[0061] Test 2: The test site is a poultry company in Taoxu Town, Hengzhou City, Nanning City, Guangxi Zhuang Autonomous Region, and the test subjects are breeder feed and broiler feed. The test time is from April 7, 2024 to April 12, 2024. During the test, the average temperature of the test site is 28.9℃ and the average humidity is 88.4%. Each chicken feed is 8 kg, and each feed is divided into 8 groups on average, with 1 kg of feed in each group; among them, test groups 1 to test groups 5 respectively use the chicken anti-stress immune enhancer prepared in Examples 1 to 5 under high temperature and high humidity conditions to add to the feed at an addition amount of 100 ppm, and control group 1 uses the chicken anti-stress immune enhancer prepared in Comparative Example 1 under high temperature and high humidity conditions to add to the feed at an addition amount of 100 ppm, and control group 2 uses the Chinese medicine composition prepared in Comparative Example 2 to add to the feed at an addition amount of 100 ppm, and the blank group is not added. The feed of each group was opened and placed in the air, and the feed situation was observed every day. The results are shown in Table 2.

[0062] Table 2: Feed situation of each group According to the data in Table 2, the chicken anti-stress immunity enhancer of the present invention mixed with feed under high temperature and high humidity conditions can effectively prevent the feed from becoming moldy, thereby preventing the problem of chickens inducing diseases due to eating moldy feed.

[0063] Test 3 The test site is a poultry company in Taoxu Town, Hengzhou City, Nanning City, Guangxi Zhuang Autonomous Region. The test object is broiler drinking water. The test time is from April 7, 2024 to April 14, 2024. During the test, the average temperature of the breeding site is 28.9 ° C and the average humidity is 88.4%; a building with 8,000 broiler breeders in the company's chicken farm is randomly selected and randomly divided into 8 groups. Each group uses a water line pipeline for water supply, and drinking water is supplied for 8 hours a day. Among them, the test groups 1 to 5 respectively use the chicken anti-stress immune enhancer prepared in Examples 1 to 5 under high temperature and high humidity conditions to add to drinking water at an addition amount of 150 ppm, the control group 1 uses the chicken anti-stress immune enhancer prepared in Comparative Example 1 under high temperature and high humidity conditions to add to drinking water at an addition amount of 150 ppm, and the control group 2 uses the Chinese medicine composition prepared in Comparative Example 2 to add to drinking water at an addition amount of 150 ppm, and the blank group does not add. After the test, the water pipes of each group were inspected and flushed to observe and record whether there were any debris and identify whether the debris was a biofilm. The results are shown in Table 3.

[0064] Table 3: Drinking water conditions in each group From the data in Table 3, it can be seen that the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention has an antibacterial effect itself, and when added to drinking water, it can effectively curb pathogenic microorganisms in the water.

[0065] Test 4 The test site is located at the poultry research and breeding base of Shaobo Town, Jiangdu District, Yangzhou City, under the Poultry Research Institute of the Chinese Academy of Agricultural Sciences. The test subjects are 410-day-old Hailan laying hens. 8,000 Hailan laying hens were randomly selected and divided into 8 groups for comparative tests. The test time is from June 20, 2024 to July 18, 2024. The average temperature of the breeding base during the test is 29.7°C and the average humidity is 87.9%. Among them, the test groups 1 to 5 respectively use the chicken anti-stress immune enhancer prepared in Examples 1 to 5 under high temperature and high humidity conditions to add to the feed at an addition amount of 100 ppm, the control group 1 uses the chicken anti-stress immune enhancer prepared in Comparative Example 1 under high temperature and high humidity conditions to add to the feed at an addition amount of 100 ppm, the control group 2 uses the Chinese medicine composition prepared in Comparative Example 2 to add to the feed at an addition amount of 100 ppm, and the feed of the control group 3 is not added. During the test, the chicken house is cooled and dehumidified by longitudinal ventilation with a water curtain fan. The eggs were fed continuously for 4 weeks, and the egg production was recorded during the experiment. The cost of use was calculated. The cost of use = total cost of each group of drugs during the experiment / the initial number of eggs in the experiment. The results are shown in Table 4.

[0066] Table 4: Egg production of each group of Hy-Line laying hens According to the data in Table 4, the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention can reduce the number of deaths of chickens under high temperature and high humidity conditions, improve the egg production rate and egg quality, reduce the number of broken shells, soft shells and sandy shells, and greatly reduce the cost of use.

[0067] Test 5 The test site is located at the Poultry Research Institute of the Chinese Academy of Agricultural Sciences, located in the Poultry Research and Breeding Base of Shaobo Town, Jiangdu District, Yangzhou City. The test subjects are 210-day-old Qingyuan Ma chicken breed roosters. 4,000 Qingyuan Ma chicken breed roosters were randomly selected and divided into 8 groups for comparative tests. The test time is from June 20, 2024 to July 18, 2024. The average temperature of the breeding base during the test is 29.7°C and the average humidity is 87.9%. Among them, the test groups 1 to 5 respectively use the chicken anti-stress immune enhancer prepared in Examples 1 to 5 under high temperature and high humidity conditions to add to the feed at an addition amount of 100 ppm, the control group 1 uses the chicken anti-stress immune enhancer prepared in Comparative Example 1 under high temperature and high humidity conditions to add to the feed at an addition amount of 100 ppm, the control group 2 uses the Chinese medicine composition prepared in Comparative Example 2 to add to the feed at an addition amount of 100 ppm, and the feed of the control group 3 is not added. During the test, the chicken house is cooled and dehumidified by longitudinal ventilation with a water curtain fan. Feeding was continued for 4 weeks, and the feed intake, semen collection, and sperm density during the experiment were recorded and the use cost was calculated. The use cost = total cost of each group of drugs during the experiment / the initial number of animals in the experiment; the results are shown in Table 5.

[0068] Table 5: Situation of each group According to the data in Table 5, the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention can reduce the number of deaths of chickens under high temperature and high humidity conditions, and effectively increase the feed intake, sperm amount and sperm density of the chickens, that is, improve the sperm quality.

[0069] Experiment 6 The test site is located in a breeder farm of a poultry company in Taoxu Town, Hengzhou City, Nanning City, Guangxi Zhuang Autonomous Region, located in Guitou Village. The test subjects are 35-week-old Taoxu hens for breeding. The test time is from April 12, 2024 to May 10, 2024. During the test, the average temperature of the breeding site is 30.1°C and the average humidity is 87.6%. 4,800 Taoxu hens were selected from the breeder farm and randomly divided into 8 groups. The test groups 1 to 5 respectively use the chicken anti-stress immune enhancer prepared in Examples 1 to 5 under high temperature and high humidity conditions to add to the feed at an addition amount of 100 ppm. The control group 1 uses the chicken anti-stress immune enhancer prepared in Comparative Example 1 under high temperature and high humidity conditions to add to the feed at an addition amount of 100 ppm; the control group 2 uses the Chinese medicine composition prepared in Comparative Example 2 to add to the feed at an addition amount of 100 ppm; the feed of the control group 3 is not added, and the chicken house is cooled and dehumidified by longitudinal ventilation with a water curtain fan during the test. Each group was fed continuously for 4 weeks. The conditions of the experimental chickens and the breeding eggs were recorded during the experiment and the use cost was calculated. The use cost = total drug cost for each group during the experiment / the initial number of experimental chickens; the results are shown in Table 6.

[0070] Table 6: Situation of each group According to the data in Table 6, the chicken anti-stress immunity enhancer under high temperature and high humidity conditions of the present invention can reduce the number of deaths of chickens under high temperature and high humidity conditions, and increase the fertilized eggs of breeding eggs, the hatching rate of fertilized eggs and the healthy chick rate.

[0071] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and the embodiments. They can be applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily realized.

Claims

1. An anti-stress immunity enhancer for chickens under high temperature and high humidity conditions, characterized in that: The invention comprises the following raw materials in parts by weight: 40-55 parts of pine needles, 35-45 parts of Cynomorium songaricum, 30-35 parts of yucca, 25-35 parts of watermelon peel, 20-30 parts of banana peel, 15-25 parts of Belamcanda chinensis, 15-23 parts of pitaya peel, 10-15 parts of Acanthopanax senticosus peel, and 5-10 parts of mulberry leaves.

2. The method for preparing the chicken anti-stress immunity enhancer under high temperature and high humidity conditions as claimed in claim 1, characterized in that: include: Obtain the following raw materials in parts by weight: 40-55 parts of pine needles, 35-45 parts of cynomorium songaricum, 30-35 parts of yucca, 25-35 parts of watermelon peel, 20-30 parts of banana peel, 15-25 parts of belamcanda chinensis, 15-23 parts of pitaya peel, 10-15 parts of acanthopanax senticosus peel, and 5-10 parts of mulberry leaves; The raw materials are sequentially washed, dried, and crushed, and then added with boiling water to stir into a paste, and then frozen at -25°C for at least 24 hours, and then transferred to room temperature to thaw to obtain a freeze-thawed material; The frozen-thawed material was subjected to organic extraction and vacuum concentration in sequence to obtain a clear paste with a density of 1.18 to 1.25; The clear paste and the organic acid are mixed in a mass ratio of 1:16.7 to obtain the chicken anti-stress immunity enhancer under high temperature and high humidity conditions.

3. The method for preparing the chicken anti-stress immune enhancer under high temperature and high humidity conditions as claimed in claim 2, characterized in that: The organic extraction specifically comprises: The freeze-thawed material was mixed with 95% ethanol solution in a volume ratio of 1:3, extracted at room temperature for 3 hours, and filtered at least 3 times. The filtrates were combined and the ethanol was evaporated by vacuum distillation.

4. The method for preparing the chicken anti-stress immunity enhancer under high temperature and high humidity conditions as claimed in claim 2, characterized in that: The vacuum concentration specifically includes: The organically extracted extract was placed in a vacuum rotary apparatus and concentrated in a water bath at a constant temperature of 60°C until the relative density reached 1.18-1.

25.

5. The method for preparing the chicken anti-stress immune enhancer under high temperature and high humidity conditions as claimed in claim 2, characterized in that: The organic acid is obtained by mixing methionine, taurine, formic acid, lactic acid and citric acid in a mass ratio of 1:1.2:1.5:0.6:0.

3.

6. Use of the chicken anti-stress immunity enhancer under high temperature and high humidity conditions as claimed in claim 1 in preventing and treating chicken stress reactions under high temperature and high humidity environments.

7. The use according to claim 6, characterized in that include: For chickens aged 1 to 2 weeks, add 500 ppm to chicken feed for feeding; For chickens aged 3 to 4 weeks, add 200 ppm to chicken feed for feeding; For chickens over 5 weeks old, add 100 ppm to chicken feed.

8. The use according to claim 6, characterized in that include: For 1-2 week old chickens, add 2500 ppm to drinking water twice a week, each time for 8 hours; For chickens aged 3-4 weeks, add 1500 ppm to drinking water twice a week, each time for 8 hours; For chickens over 5 weeks old, add 500 ppm to drinking water twice a week, each time for 8 hours.

9. The use according to claim 6, characterized in that include: For chickens aged 1 to 2 weeks, add 700 ppm to drinking water once a day for 8 hours each time. For chickens aged 3 to 4 weeks, add 400 ppm to drinking water once a day for 8 hours each time. For chickens over 5 weeks old, add 150 ppm to drinking water once a day for 8 hours each time.

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