Automatic preparation device for microbial culture medium

Abstract

The invention relates to the related technical field of culture medium preparation, discloses an automatic preparation device for a microbial culture medium, and aims to solve the problem of component pollution caused by exposure of the culture medium in the external environment in the preparation process. The medicine feeding cylinder is used for penetrating into the injection plug, dropper feeding is replaced with an injection mode, external environment pollution is avoided, and meanwhile accurate feeding can be achieved through scales on the medicine feeding cylinder. After all the components of the culture medium are injected, a motor is used for driving the central disc to rotate, so that the bevel gear on the central disc rotates along the fixed gear ring, the culture medium test tube fixed on the bevel gear continuously rotates, and all the components in the culture medium test tube are mixed; finally, the effects of batching in a sterile environment and rotary stirring of the test tube are achieved.

Description

Technical Field

[0001] The invention relates to the technical field related to culture medium preparation, and in particular to an automatic preparation device for microbial culture medium. Background Art

[0002] The design of automatic preparation of microbial culture medium is to facilitate operators to complete the preparation, mixing, and packaging of culture medium, so as to improve the experimental efficiency and the quality of culture medium. Automatic preparation of microbial culture medium has a wide range of applications in many fields, such as microbiology research, medical diagnosis, environmental monitoring, food industry, drug development, and genetic engineering.

[0003] At present, the preparation of microbial culture medium still mainly relies on manual operation, for example, using a dropper to inject the culture medium into a hose. However, this method is difficult to ensure the accurate preparation of each sample, the error is large, and it cannot meet the needs of high-precision experiments. In addition, during the preparation process, in order to ensure the full mixing of different culture medium components, it is often necessary to use a stirring rod to stir to ensure the uniformity of the various components of the culture medium. However, in actual applications, we found that the culture medium usually needs to be kept sterile to prevent contamination by external environmental factors. Both the dropper injection and the stirring rod stirring methods will expose the culture medium to the external environment, thereby increasing its risk of contamination. Once the culture medium is contaminated, it will not only lead to component loss, but may also cause component denaturation, seriously affecting the accuracy of the experimental results. Summary of the invention

[0004] The present invention provides an automatic microbial culture medium preparation device, which has the advantages of aseptic environment preparation and test tube rotation stirring, and is used to solve the problem of component contamination caused by leakage of culture medium into the external environment during preparation mentioned in the background technology.

[0005] To achieve the above-mentioned purpose, the present invention adopts the following technical scheme: an automatic preparation device for microbial culture medium, comprising: a mounting base, a center disk driven by a motor is arranged on the inner top; a bevel gear, a rotating shaft is movably mounted on the outer side of the center disk, and a fixed gear ring meshing with the bevel gear is fixedly mounted on the top of the mounting base; a culture medium test tube, which is fastened to the rotating shaft of the bevel gear through a test tube fixing frame, a test tube cap is inserted into the top of the culture medium test tube, and a filling plug is fixedly mounted on the top of the test tube cap; a medicine dispensing ring frame, which is movably mounted on the top of the center disk, and a medicine delivery cartridge is arranged on the surface of the medicine dispensing ring frame, and a return push spring is arranged between the medicine delivery cartridge and the medicine dispensing ring frame.

[0006] Furthermore, there are multiple drug delivery cartridges, which are arranged in a circular shape at equal angles on the surface of the drug dispensing ring frame, and each drug delivery cartridge contains culture medium of different components.

[0007] Furthermore, a tightening wedge block is fixedly connected to the bottom of the test tube cover, a tightening screw is threadedly connected to the test tube cover, and a tightening ring is movably installed at the bottom of the tightening screw.

[0008] Furthermore, a weight column is placed inside the culture medium test tube.

[0009] Furthermore, guide buffer sliders are fixedly installed at both ends of the counterweight column, and a stirring rod is arranged on the outside of the counterweight column.

[0010] Furthermore, a jet cylinder is fastened to the inner side of the test tube cover, a jet piston is mounted inside the jet cylinder, one end of a piston driving arm is fastened to the top of the jet piston, and the other end is fastened to a magnetic block; a jet hole is opened on the side of the piston driving arm, and the jet hole is communicated with the inner cavity of the jet cylinder through the inner cavity of the piston driving arm.

[0011] Furthermore, a one-way air intake valve is fixed on the top of the air jet cylinder.

[0012] Furthermore, a detection cylinder is threadedly connected to the top of the test tube cover, and a gas supply pipe is fastened to the inner side of the test tube cover; a detection piston is movably installed inside the detection cylinder, and a detection spring is connected between the detection piston and the detection cylinder.

[0013] Furthermore, a one-way air supply valve is fixedly installed on the surface of the detection piston.

[0014] The present invention has the following beneficial effects: The present invention provides an automatic microbial culture medium preparation device, the core component of which is a medicine dispensing ring frame, on which a plurality of medicine delivery cartridges containing different culture medium components are carefully arranged. These medicine delivery cartridges not only store various required components, but also take into account the requirements of aseptic operation in their design, thus avoiding the risk of external environmental contamination that may be caused by traditional dropper feeding.

[0015] When the culture medium needs to be prepared, the drug delivery cartridge is controlled to accurately penetrate the injection plug and the culture medium components are delivered into the test tube by injection. This process is not only fast and efficient, but also with the help of the clearly marked scale on the drug delivery cartridge, it can ensure the accuracy of each delivery, thereby greatly improving the accuracy and consistency of culture medium preparation.

[0016] After the components of the culture medium are successfully injected into the test tube, the motor is started to drive the center disk to start rotating. The bevel gear on the center disk then rotates along the fixed gear ring. This design cleverly utilizes the principle of gear transmission, allowing the culture medium test tube fixed on the bevel gear to rotate smoothly and continuously. Through the continuous rotation of the test tube, the culture medium components in the test tube are fully mixed and evenly, achieving an efficient and sterile mixing process.

[0017] In summary, the automatic microbial culture medium preparation device not only achieves accurate batching in a sterile environment, but also ensures the full mixing of the culture medium components through an innovative test tube rotation and stirring method. The application of this device will greatly improve the efficiency and quality of microbiology experiments and provide strong support for scientific research and production. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings, which constitute a part of the specification, illustrate embodiments of the present disclosure and, together with the description, serve to explain the principles of the present disclosure.

[0019] The present invention may be more clearly understood from the following detailed description with reference to the accompanying drawings, in which: Figure 1 It is a schematic diagram of the overall external three-dimensional structure of the present invention; Figure 2 It is a three-dimensional schematic diagram of the drug dispensing structure in the present invention; Figure 3 This is a schematic diagram of the center disk installation and drive structure; Figure 4 It is a three-dimensional schematic diagram of the installation position of the culture medium test tube; Figure 5 This is a schematic diagram of the external three-dimensional structure of the culture medium test tube; Figure 6 It is a schematic diagram of the internal cross-sectional three-dimensional structure of the culture medium test tube; Figure 7 for Figure 6 The enlarged structural diagram at E in the middle; Figure 8 for Figure 6 Enlarged structural diagram at F in the middle.

[0020] In the figure: 1. mounting base; 2. fixed gear ring; 3. dispensing ring frame; 4. medicine delivery cartridge; 5. return push spring; 6. center plate; 7. bevel gear; 8. test tube fixing frame; 9. culture medium test tube; 10. motor; 11. test tube cover; 110. injection plug; 111. tightening wedge block; 12. tightening ring; 120. tightening screw; 13. detection cartridge; 14. counterweight column; 140. guide buffer slider; 141. stirring rod; 15. detection piston; 150. detection spring; 151. one-way air supply valve; 16. air supply pipe; 17. jet cylinder; 171. one-way air intake valve; 18. jet piston; 19. piston drive arm; 190. jet hole; 20. magnetic block. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] For example, see Figure 1 and Figure 3 It can be seen that the mounting base 1 is fixed at the desired position according to the flange at the bottom, a motor 10 is fixedly mounted on the top of the inner side of the mounting base 1, and a center disk 6 located at the top of the mounting base 1 is fixedly mounted on the output shaft of the motor 10, and the center disk 6 can be driven to rotate by the motor 10. A plurality of bevel gears 7 distributed at equal angles are circumferentially mounted on the outer side of the center disk 6, and correspondingly, a fixed gear ring 2 meshing with the bevel gear 7 is fixedly mounted on the top of the mounting base 1. When the motor 10 causes the bevel gear 7 to rotate circumferentially around the fixed gear ring 2 through the center disk 6, the meshing between the bevel gear 7 and the fixed gear ring 2 forces the bevel gear 7 to rotate on its own.

[0023] The culture medium test tube 9 is fastened to the rotating shaft of the bevel gear 7 through the test tube fixing frame 8. Figure 3 and Figure 4 It can be clearly seen that the test tube fixing frame 8 is composed of two elastic clamping blocks, wherein one clamping block is fixed on the bevel gear 7 and the other is sleeved on the screw rod. When the culture medium test tube 9 needs to be fixed, the culture medium test tube 9 is placed between the two clamping blocks, and the bolt on the screw rod is turned to make the two clamping blocks relatively close, and finally the culture medium test tube 9 between the clamping blocks is clamped and tightened.

[0024] Furthermore, in order to prevent the material inside the culture medium test tube 9 from being poured out during the rotation process, a test tube cover 11 is inserted through the top of the culture medium test tube 9, thereby sealing the inner cavity of the culture medium test tube 9 and preventing the raw materials inside the culture medium test tube 9 from being contaminated by the external environment. Figure 4 and Figure 5 It can be seen that a filling plug 110 is fixedly installed on the top of the test tube cover 11. The filling plug 110 is generally made of rubber. When the needle on the drug delivery cartridge 4 pierces the filling plug 110, each component of the culture medium can be injected in a sterile environment. Figure 1 and Figure 2It can be clearly seen that there is a medicine dispensing ring frame 3 connected by a bearing on the top of the center disk 6, and a plurality of medicine delivery barrels 4 are arranged at equal angles on the surface of the medicine dispensing ring frame 3. A return spring 5 is arranged between each medicine delivery barrel 4 and the medicine dispensing ring frame 3. Under normal conditions, the medicine delivery barrel 4 is pushed by the elastic force of the return spring 5 to force the medicine delivery barrel 4 to move upward, so that the needle at the bottom of the medicine delivery barrel 4 is relatively far away from the culture medium test tube 9. When it is necessary to prepare the culture medium, the medicine delivery barrel 4 of the required component is moved to the top of the corresponding culture medium test tube 9, and the medicine delivery barrel 4 is squeezed downward and the return spring 5 is compressed until the needle at the bottom of the medicine delivery barrel 4 pierces the injection plug 110, so that the culture medium component in the medicine delivery barrel 4 is injected into the inner cavity of the culture medium test tube 9 (the culture medium injection method of other components is the same). In this process, since the culture medium is completely isolated from the outside, the contamination of the culture medium component by the external environment is greatly avoided. In addition, a scale line is arranged on the outside of the medicine delivery barrel 4, so that the operator can accurately know the injection amount of the culture medium component, and ensure that the access component is relatively accurate each time.

[0025] Specifically, during the preparation of the culture medium, the operator rotates the medicine dispensing ring frame 3 and moves the required medicine delivery barrel 4 to the top of the culture medium test tube 9. After pressing the medicine delivery barrel 4 downward, the needle at the bottom of the medicine delivery barrel 4 will pierce the injection plug 110 and enter the inner cavity of the culture medium test tube 9, so that the culture medium components in the medicine delivery barrel 4 are aseptically injected. When the injection is completed, the medicine delivery barrel 4 is relatively away from the culture medium test tube 9 under the elastic force of the return push spring 5, that is, the input of the culture medium components is completed, and the input method of other culture medium components is the same.

[0026] After culture media of different components are input into the culture media test tube 9, the motor 10 is used to drive the center disk 6 to rotate, thereby causing the center disk 6 to drive the bevel gear 7 to rotate circumferentially. During this process, the bevel gear 7 rotates around the fixed gear ring 2, thereby driving the culture media test tube 9 to rotate, so that the various components inside the culture media test tube 9 are evenly mixed, thereby ensuring that the culture media raw materials inside the culture media test tube 9 can be mixed and stirred under sterile conditions.

[0027] Finally, when the culture media of each component in the culture media test tube 9 is completely stirred, the culture media test tube 9 can be taken out from the test tube fixing rack 8 .

[0028] On this basis, in order to prevent the test tube cover 11 from accidentally detaching from the end of the culture medium test tube 9, Figure 5-Figure 7It can be clearly seen that the bottom of the test tube cover 11 is fixedly connected with a tightening wedge block 111 located on the outside of the culture medium test tube 9, and the tightening wedge block 111 is preferably a rubber product. There are three tightening wedge blocks 111, and the three tightening wedge blocks 111 are distributed in a circular shape at equal angles at the bottom of the test tube cover 11. A tightening screw 120 is threadedly connected to the test tube cover 11. When the tightening screw 120 is turned, it will push the tightening ring 12 installed movably at the bottom to move downward synchronously. Since the inner ring of the tightening ring 12 is located on the outside of the tightening wedge block 111, when the tightening ring 12 is continuously pushed downward, combined with the outer side of the tightening wedge block 111 being an inclined surface, the squeezing strength of the tightening ring 12 on the outer side of the tightening wedge block 111 will continue to increase, and the tightening wedge block 111 will be tightly attached to the outer side of the culture medium test tube 9. The compression of the tightening ring 12 increases the friction between the tightening wedge block 111 and the culture medium test tube 9 , thereby preventing the test tube cover 11 from accidentally falling off from the end of the culture medium test tube 9 .

[0029] Example 2 is a further improvement on Example 1. In order to make the culture medium of each component in the culture medium test tube 9 uniformly mixed, please refer to Figure 6 It can be seen that a counterweight column 14 is placed inside the culture medium test tube 9. When the culture medium test tube 9 rotates, the counterweight column 14 moves in the culture medium test tube 9 to enhance the stirring of the culture medium of each component in the inner cavity of the culture medium test tube 9. In addition, since the counterweight column 14 is made of metal, in order to prevent the counterweight column 14 from being damaged when it hits the culture medium test tube 9, a guide buffer slider 140 is fixedly installed at both ends of the counterweight column 14. The guide buffer slider 140 enables the counterweight column 14 to move in a directional manner in the culture medium test tube 9, thereby avoiding direct collision between the counterweight column 14 and the culture medium test tube 9. At the same time, a plurality of stirring rods 141 are arranged on the outside of the counterweight column 14. The stirring rods 141 can further enhance the stirring of the culture medium of each component in the culture medium test tube 9, thereby further enhancing the mixing efficiency of the raw materials of the culture medium of each component in the inner cavity of the culture medium test tube 9.

[0030] On this basis, combined with Figure 6-Figure 8 It can be seen that the inner side of the test tube cover 11 has a jet cylinder 17 coaxially connected to the connecting plate. The jet cylinder 17 is in the shape of a hollow cylinder, and a jet piston 18 is mounted inside the jet cylinder. The piston drive arm 19 is fastened to the top of the jet piston 18. Figure 8It can be clearly seen that the piston driving arm 19 is in a U-shape, one end of the piston driving arm 19 passes through the jet cylinder 17 and is connected to the jet piston 18, and the other end is located outside the jet cylinder 17, and the end is fastened with a magnetic block 20. Under normal conditions, the magnetic block 20 will attract the counterweight column 14, forcing the two to have a tendency to be relatively close. Furthermore, a plurality of jet holes 190 are opened on the side of the piston driving arm 19, and the jet holes 190 are directly connected through the inner cavity of the piston driving arm 19 and the inner cavity of the jet cylinder 17, such as Figure 8 As shown, when the jet piston 18 moves upward, the airflow in the inner cavity of the jet cylinder 17 and located at the top of the jet piston 18 will be output from the jet hole 190; similarly, when the jet piston 18 moves downward, the airflow inside the culture medium tube 9 will be unidirectionally transported from the one-way suction valve 171 fixed on the top of the jet cylinder 17 to the inner cavity of the jet cylinder 17.

[0031] Specific, combined Figure 1 As shown, when the culture medium test tube 9 is in a relatively vertical state, the test tube cover 11 is located above the end of the culture medium test tube 9. Figure 6 and Figure 8 As shown, the counterweight column 14 is located at the bottom of the inner cavity of the culture medium test tube 9 under the action of its own gravity, and the magnetic attraction of the magnetic block 20 to the counterweight column 14 forces the piston drive arm 19 to pull the jet piston 18 to move downward. At this time, the inner cavity of the jet cylinder 17 is located above the jet piston 18 to store a large amount of air.

[0032] As the motor 10 drives the center disk 6 to rotate, the bevel gear 7 drives the culture medium test tube 9 to rotate according to the fixed gear ring 2, and the test tube cover 11 tends to rotate to the bottom of the culture medium test tube 9. In this process, as the culture medium test tube 9 rotates 180°, the counterweight column 14 also tends to move toward the test tube cover 11 due to the rotation of the culture medium test tube 9. When the counterweight column 14 moves along the inner cavity of the culture medium test tube 9, it stirs the inner cavity of the culture medium test tube 9 by using the stirring rod 141 fixed on its outer side, thereby completing the mixing of the culture media of each component in the inner cavity of the culture medium test tube 9. In addition, when the counterweight column 14 approaches the test tube cover 11, it will first contact the magnetic block 20, thereby pushing the piston drive arm 19 to move. At this time, the piston drive arm 19 pulls the jet piston 18 to move toward the one-way air intake valve 171, and the jet piston 18 squeezes the air in the inner cavity of the jet cylinder 17 and discharges it from the jet hole 190. At this time, the air jet hole 190 is immersed in the culture medium, and the bubbles bulging out from the air jet hole 190 realize the disturbance of the culture medium in the inner cavity of the culture medium test tube 9, further enhancing the mixing of the various component culture media in the inner cavity of the culture medium test tube 9.

[0033] As the culture medium test tube 9 rotates further, the culture medium test tube 9 will rotate 180° again until the test tube cover 11 moves to the square of the culture medium test tube 9 again. At this time, the counterweight column 14 moves downward under gravity, and the magnetic block 20 pushes the jet piston 18 away from the one-way suction valve 171 through the piston drive arm 19. The air at the top of the culture medium test tube 9 enters the inner cavity of the jet cylinder 17 through the one-way suction valve 171, and after waiting for the next rotation, the jet piston 18 is used to push the airflow in the jet cylinder 17 outward.

[0034] Example 3 is a supplement to Example 2. Figure 5-Figure 8 It can be seen that the top of the test tube cover 11 is threadedly connected with a detection tube 13, and the inner side of the test tube cover 11 is fastened with an air delivery pipe 16 for connecting the inner cavity of the detection tube 13 with the inner cavity of the air injection tube 17. Figure 8 It can be seen that when the jet piston 18 moves to the limit away from the one-way air inlet valve 171, the jet cylinder 17 communicates with the inner cavity of the detection cylinder 13 through the air supply pipe 16. The detection cylinder 13 is movably equipped with a detection piston 15, so that the inner cavity of the detection cylinder 13 and the bottom of the detection piston 15 form a relatively sealed area. In addition, a detection spring 150 is connected between the detection piston 15 and the detection cylinder 13. Under the push of the detection spring 150, the detection piston 15 moves downward and approaches the air supply pipe 16. On this basis, combined with Figure 7 It can be seen that a one-way air supply valve 151 is fixedly installed on the surface of the detection piston 15 , and the one-way air supply valve 151 ensures that external air can only be input into the inner cavity of the detection cylinder 13 .

[0035] During specific preparation, the test tube cover 11 is inserted into the top of the culture medium test tube 9 , and the tightening ring 12 is pressed tightly against the tightening wedge block 111 , so that the test tube cover 11 is firmly locked on the top of the culture medium test tube 9 .

[0036] Under normal circumstances, the culture medium test tube 9 is arranged vertically and the test tube cover 11 is at the top of the culture medium test tube 9. At this time, the counterweight column 14 is located at the bottom of the inner cavity of the culture medium test tube 9, and the magnetic block 20 attracts the counterweight column 14, forcing the magnetic block 20 to push the jet piston 18 downward away from the one-way suction valve 171 according to the piston drive arm 19 until the jet piston 18 passes over the air supply pipe 16. At this time, the inner cavity of the jet cylinder 17 is connected to the detection cylinder 13 through the air supply pipe 16.

[0037] Afterwards, the needle at the bottom of the drug delivery barrel 4 is used to pierce the injection plug 110, and the culture medium components in the inner cavity of the drug delivery barrel 4 are injected into the culture medium test tube 9. In this process, the injected culture medium will cause the inner cavity pressure of the culture medium test tube 9 to increase, and the air in the inner cavity of the culture medium test tube 9 will be compressed, which will force the inner cavity pressure of the detection barrel 13 connected to it to increase synchronously, thereby pushing the detection piston 15 upward and compressing the detection spring 150. At this time, the detection piston 15 has a tendency to move upward. After all components of the culture medium are injected into the inner cavity of the culture medium test tube 9, the internal pressure of the culture medium test tube 9 is also relatively increased, and the detection piston 15 moves up to a certain height. At this time, by checking whether the detection piston 15 rises, the outside personnel can clearly know whether the inside of the culture medium test tube 9 is in a relatively sealed state, so as to judge whether the culture medium in the inner cavity of the culture medium test tube 9 is connected to the external environment and contaminated. Specifically, if the detection piston 15 rises, it means that the inner cavity of the culture medium test tube 9 is airtight; otherwise, it means that there is a leakage problem in the culture medium test tube 9.

[0038] As the motor 10 drives the center disk 6 to rotate, the culture medium test tube 9 will rotate. After the culture medium test tube 9 rotates 180°, the counterweight column 14 moves toward the test tube cover 11 and contacts the extrusion piston drive arm 19. The piston drive arm 19 pulls the jet piston 18 across the gas pipe 16. At this time, the airflow in the inner cavity of the jet cylinder 17 is squeezed by the jet piston 18 and output from the jet hole 190 to the culture medium in the inner cavity of the culture medium test tube 9. The bubble disturbance is used to further enhance the intensity of the culture medium mixing.

[0039] If there is a leakage between the culture medium test tube 9 and the test tube cover 11 during use, at this time, when the culture medium test tube 9 is arranged inverted, the test tube cover 11 is located at the bottom of the culture medium test tube 9, and as the jet piston 18 presses the airflow in the inner cavity of the jet cylinder 17 into the inner cavity of the culture medium test tube 9, the airflow enters the inner cavity of the culture medium test tube 9, causing its air pressure to increase. Combined with the fact that the test tube cover 11 is located at the bottom at this time, the culture medium will flow out directly along the leakage site, thereby alerting the operator that there is a leakage problem in the culture medium test tube 9 here.

[0040] As the culture medium test tube 9 continues to rotate, the culture medium test tube 9 rotates 180° again. At this time, the test tube cover 11 is located at the top of the culture medium test tube 9. The magnetic block 20 pulls the jet piston 18 away from the one-way air inlet valve 171 through the piston drive arm 19 under the premise of attracting the counterweight column 14, until the jet piston 18 passes over the air supply pipe 16. Since the airflow in the inner cavity of the detection cylinder 13 is squeezed by the detection spring 150, when the inner cavity of the detection cylinder 13 and the jet cylinder 17 are connected, the airflow in the jet cylinder 17 will continue to be discharged along the jet hole 190. Combined with the leakage between the culture medium test tube 9 and the test tube cover 11 at this time, the airflow in the air supply pipe 16 is continuously discharged outward until the detection piston 15 moves to the bottom of the detection cylinder 13.

[0041] When the leaking culture medium test tube 9 rotates 180 degrees again, the test tube cover 11 rotates to the bottom of the culture medium test tube 9, and the jet piston 18 squeezes the air in the inner cavity of the jet cylinder 17 again, causing the pressure in the inner cavity of the culture medium test tube 9 to increase, further exacerbating the discharge of the culture medium in the culture medium test tube 9 from the leaking part. In addition, when the jet piston 18 moves upward, the inner cavity of the jet cylinder 17 at the bottom of the jet piston 18 will reduce pressure, so that the external air flow is sucked into the jet cylinder 17 through the one-way air supply valve 151 through the air pipe 16, thereby reducing the movement resistance of the jet piston 18.

[0042] Finally, when the stirring of the culture medium test tube 9 is finished, if the detection piston 15 does not move to the bottom of the detection cylinder 13, it means that the inner cavity of the culture medium test tube 9 is still in a relatively sealed state, and the culture medium in the culture medium test tube 9 is not connected to the outside, and there is no problem of being polluted by the external environment; on the contrary, if the detection piston 15 reaches the bottom of the detection cylinder 13, it means that the pressure inside the culture medium test tube 9 is consistent with the outside, and there is a leakage, and the culture medium inside the culture medium test tube 9 may be contaminated. In addition, if the leakage site is located between the culture medium test tube 9 and the test tube cover 11, the culture medium raw material inside the culture medium test tube 9 is discharged, so that the leakage site can be determined, which is convenient for subsequent personnel to quickly find the leakage point for repair.

Claims

1. A device for automatically preparing a microbial culture medium, characterized in that: include: A mounting base (1) is provided with a center disk (6) driven by a motor (10) on the inner top; A bevel gear (7) whose rotating shaft is movably mounted on the outer side of the center disk (6), and a fixed gear ring (2) meshing with the bevel gear (7) is fixedly mounted on the top of the mounting base (1); A culture medium test tube (9) is fastened to the rotating shaft of the bevel gear (7) via a test tube fixing frame (8); a test tube cover (11) is inserted into the top of the culture medium test tube (9), and a material injection plug (110) is fixedly installed on the top of the test tube cover (11); A medicine dispensing ring frame (3) is movably mounted on the top of the central plate (6), and a medicine delivery cartridge (4) is arranged on the surface of the medicine dispensing ring frame (3), and a return push spring (5) is arranged between the medicine delivery cartridge (4) and the medicine dispensing ring frame (3); A weight column (14) is placed inside the culture medium test tube (9); Guide buffer sliders (140) are fixedly mounted on both ends of the counterweight column (14), and a stirring rod (141) is arranged outside the counterweight column (14).

2. The automatic microbial culture medium preparation device according to claim 1, characterized in that: There are a plurality of drug delivery cartridges (4), which are arranged in a ring-like shape at equal angles on the surface of the drug dispensing ring frame (3), and each drug delivery cartridge (4) contains a culture medium of different components.

3. The automatic microbial culture medium preparation device according to claim 1, characterized in that: A tightening wedge block (111) is fixedly connected to the bottom of the test tube cover (11), a tightening screw rod (120) is threadedly connected to the test tube cover (11), and a tightening ring (12) is movably mounted on the bottom of the tightening screw rod (120).

4. The automatic microbial culture medium preparation device according to claim 1, characterized in that: The inner side of the test tube cover (11) is fastened with a jet cylinder (17), a jet piston (18) is mounted inside the jet cylinder (17), one end of a piston driving arm (19) is fastened to the top of the jet piston (18), and the other end is fastened with a magnetic block (20); A jet hole (190) is provided on the side of the piston driving arm (19), and the jet hole (190) is communicated with the inner cavity of the jet cylinder (17) through the inner cavity of the piston driving arm (19).

5. The automatic microbial culture medium preparation device according to claim 4, characterized in that: A one-way air suction valve (171) is fixed on the top of the jet cylinder (17).

6. The automatic preparation device for microbial culture medium according to claim 4, characterized in that: The top of the test tube cover (11) is threadedly connected to a detection cylinder (13), and the inner side of the test tube cover (11) is tightly connected to a gas supply pipe (16); a detection piston (15) is movably installed inside the detection cylinder (13), and a detection spring (150) is connected between the detection piston (15) and the detection cylinder (13).

7. The automatic microbial culture medium preparation device according to claim 6, characterized in that: A one-way air supply valve (151) is fixedly mounted on the surface of the detection piston (15).

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