Method for preparing high-content curcumin extract from Dai medicine purple ginger

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a method for preparing a high-content curcumin extract from Dai medicine purple ginger. Compared with the prior art, the method has the advantages that the purple ginger is selected as a high-quality original plant of curcumin components, and the curcumin extract is prepared by screening the growth age limit, the processing mode and the extraction mode of the purple ginger; specific processing conditions are optimized, and under the conditions, the extraction amount of curcumin in the purple ginger can reach 386.23 mg / 100g and is obviously higher than that of zingiber corallinum hance and rhizoma curcumae longae; the purple ginger industrial chain can be further expanded, the additional value is improved, and a theoretical basis is provided for comprehensive utilization of purple ginger.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine, and in particular to a method for preparing a high-content curcumin extract from purple ginger, a Dai medicine. Background Art

[0002] Dai medicine Bu Lei is a traditional and commonly used medicinal material of the Dai people. Its original plants include purple ginger and coral ginger. Purple ginger is the rhizome of purple ginger, a plant of the ginger family. It tastes spicy and is hot in nature. It enters the spleen, stomach, heart, and lung meridians. Purple ginger is one of the traditional commonly used medicines of the Dai people in Xishuangbanna. It has the effects of relaxing gastrointestinal smooth muscles, relieving spasmodic pain, and increasing gastric motility. The rhizome is ground into vinegar and applied externally to treat stubborn skin eczema and other diseases. It has unique therapeutic effects in the treatment of various fungal skin diseases and skin health care, and has broad application prospects. Domestic and foreign scholars have focused their research on the chemical composition of Bu Lei on volatile oils, and have done less research on non-volatile substances. Curcuminoids, as non-volatile components, belong to polyphenol compounds and have multiple pharmacological activities, such as anti-inflammatory, anti-apoptosis, anti-aging, antioxidant, immune regulation, nerve protection, and metabolic regulation. As a natural antioxidant, curcuminoids have attracted much attention in recent years, and their application fields have been widely expanded to many industries such as food and medicine. Therefore, it is necessary to develop a method for extracting effective ingredients using the original plant purple ginger as raw material, which is of great significance to provide more original options for curcumin raw materials. Summary of the invention

[0003] In order to overcome the above technical defects, the present invention provides a method for preparing a high-content curcumin extract from Dai medicine purple ginger. The curcumin content is used as a detection index to optimize the extraction process. Under the optimized process conditions, the content differences of curcumin components in samples with different processing conditions, different varieties and different growth years are compared, so as to provide more origin options for curcumin raw materials.

[0004] To achieve the above object, the present invention is implemented through the following scheme:

[0005] S1. Take fresh purple ginger, wash it, and cut it into slices with a thickness of 2-5 mm;

[0006] S2. Dry the slices until the moisture content is less than 10%;

[0007] S3. The dried slices were crushed, 60-100% ethanol solution was added, and ultrasonic extraction was performed;

[0008] S4. After separation, the extract is used to obtain the effective components of purple ginger.

[0009] Furthermore, the purple ginger medicinal material in S1 has a growth period of ≥3 years.

[0010] Furthermore, the drying method in S2 is room temperature drying.

[0011] Furthermore, the drying condition in S2 is: drying in the shade at 25° C. until the moisture content of the purple ginger is less than 10%.

[0012] Furthermore, in S3, the concentration of the ethanol solution is 60-95%, or 70-95%, or 80-95%, or 85-95%.

[0013] Furthermore, in S3, the mass volume ratio of the medicinal material to the ethanol solution is 1 g: (30-70) ml.

[0014] Furthermore, in S3, the mass volume ratio of the medicinal material to the ethanol solution is 1g: (40-70) ml; 1g: (45-70) ml; 1g: (45-65) ml; 1g: (45-60) ml.

[0015] Furthermore, in S3, the ultrasonic extraction conditions are: ultrasonic power of 200W-600W, extraction time of 10-80min.

[0016] Further, in S3, the ultrasonic power is 300W-600W; or 400W-600W; or 350W-550W; or 350W-500W.

[0017] Furthermore, in S3, the ultrasonic time is 15-75 min; or 30-75 min; or 45-75 min; or 45-60 min.

[0018] Compared with the prior art, the present invention selects purple ginger as a high-quality source plant of curcumin components, and selects specific processing conditions by screening the growth years, processing methods and extraction methods of purple ginger. Under these conditions, the curcumin extraction amount of purple ginger can reach 386.23 mg / 100 g, which is significantly higher than that of coral ginger and turmeric. The present invention can further expand the purple ginger industry chain, enhance added value, and provide a theoretical basis for the comprehensive utilization of purple ginger.

[0019] Instruction Manual

[0020] Figure 1 The extraction rate of curcumin in purple ginger treated with different ethanol concentrations;

[0021] Figure 2 The extraction rate of curcumin from purple ginger treated with different solid-liquid ratios;

[0022] Figure 3 The extraction rate of curcumin from purple ginger treated with different ultrasonic powers;

[0023] Figure 4 Curcumin extraction rate of purple ginger treated with different ultrasonic times. DETAILED DESCRIPTION

[0024] In order to make those skilled in the art better understand the technical scheme of the present invention, the present invention is described in detail below in conjunction with specific embodiments. The experimental methods for which specific conditions are not indicated in the following examples are usually carried out under normal conditions or according to the conditions recommended by the manufacturer. The test materials used in the following examples, unless otherwise specified, are purchased from conventional biochemical reagent stores. Unless otherwise specified, percentages and parts are calculated by weight. Unless otherwise defined, all professional and scientific terms used in the text have the same meaning as those familiar to those skilled in the art. In addition, any method and material similar to or equal to the recorded content can all be applied to the present invention. The preferred implementation methods and materials described in the text are only for demonstration purposes.

[0025] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0026] Based on the present invention, the inventor selected purple ginger, a native plant of Dai medicine, as raw material, took curcumin as the detection index, and optimized the extraction process by single factor and orthogonal experiments, which specifically included the following steps:

[0027] S1. Take fresh purple ginger, wash it, and cut it into slices with a thickness of 2-5 mm;

[0028] S2. Dry the slices until the moisture content is less than 10%;

[0029] S3. The dried slices were crushed, 60-100% ethanol solution was added, and ultrasonic extraction was performed;

[0030] S4. After separation, the extract is used to obtain the effective components of purple ginger.

[0031] By comparing the differences in the content of curcumin-like components in samples under different processing conditions, different varieties, different growth years and extraction conditions, more origin options are provided for curcumin raw materials.

[0032] Based on the present invention, the growth period of the purple ginger medicinal material in S1 is ≥ 3 years.

[0033] Based on the present invention, the drying method in S2 is room temperature drying.

[0034] Based on the present invention, the drying condition in S2 is: drying in the shade at 25° C. until the moisture content of the purple ginger is less than 10%.

[0035] Based on the present invention, in S3, the concentration of the ethanol solution is 60-95%, or 70-95%, or 80-95%, or 85-95%.

[0036] Based on the present invention, in S3, the mass volume ratio of the medicinal material to the ethanol solution is 1g: (30-70)ml.

[0037] Based on the present invention, in S3, the mass volume ratio of the medicinal material to the ethanol solution is 1g: (40-70) ml; 1g: (45-70) ml; 1g: (45-65) ml; 1g: (45-60) ml.

[0038] Based on the present invention, in S3, the ultrasonic extraction conditions are: ultrasonic power of 200W-600W, extraction for 10-80min.

[0039] Based on the present invention, in S3, the ultrasonic power is 300W-600W; or 400W-600W; or 350W-550W; or 350W-500W.

[0040] Based on the present invention, in S3, the ultrasonic time is 15-75 min; or 30-75 min; or 45-75 min; or 45-60 min.

[0041] The technical solution of the present invention is further described in detail below in conjunction with specific embodiments and drawings. It should be understood that the following embodiments are only used to explain the present invention and are not used to limit the present invention.

[0042] The reagents and instruments used in the following examples are from:

[0043] Experimental materials and reagents: Reagents:

[0044]

[0045]

[0046] Example 1 Investigation of Optimal Ethanol Concentration

[0047] Take fresh purple ginger medicinal material with a growth period of 3 years, wash it, cut it into slices with a thickness of 2mm, dry it in the shade at 25℃, grind it after the water content of the purple ginger is basically evaporated, add 60%, 70%, 80%, 90%, 100% ethanol solution, the mass volume ratio of the medicinal material to the ethanol solution is 1g:40ml, extract it for 30min at an ultrasonic power of 400W; let the extract stand at room temperature, take the supernatant, filter it with a microporous filter membrane, and use HPLC to detect the content of curcuminoids. The results are shown in Table 2. Figure 1 .

[0048] like Figure 1 As shown in the figure, with the increase of ethanol concentration, the extraction rate of curcuminoids showed a trend of first increasing and then decreasing. Since curcuminoids are insoluble in water, when the water content in the solvent gradually decreases, the cell damage rate decreases, and the dissolution amount of curcuminoids also decreases accordingly. The extraction rate of curcuminoids is the highest when the ethanol concentration is 90%.

[0049] Example 2 Investigation of the Optimal Material-Liquid Ratio

[0050] Take fresh purple ginger medicinal material with a growth period of 3 years, wash it, cut it into slices with a thickness of 2mm, dry it in the shade at 25℃, grind it after the water content of the purple ginger is basically evaporated, add 90% ethanol solution, the mass volume ratio of the medicinal material to the ethanol solution is 1g:30ml, 1g:40ml, 1g:50ml, 1g:60ml, 1g:70ml, extract it for 30min at an ultrasonic power of 400W; let the extract stand at room temperature, take the supernatant, filter it with a microporous filter membrane, and use HPLC to detect the content of curcuminoids. The results are shown in Table 1. Figure 2 .

[0051] like Figure 2 As shown in the figure, the extraction rate of curcuminoids showed a trend of increasing first and then decreasing. When the solid-liquid ratio was 1:50, the extraction efficiency of curcuminoids was the highest, but when the solid-liquid ratio was too high, the material diffusion equilibrium concentration would be reached, resulting in a decrease in the extraction rate of curcumin.

[0052] Example 3 Investigation of Optimal Ultrasonic Power

[0053] Take fresh purple ginger medicinal material with a growth period of 3 years, wash it, cut it into slices with a thickness of 2 mm, dry it in the shade at 25°C, grind it after the water content of the purple ginger is basically evaporated, add 90% ethanol solution, the mass volume ratio of the medicinal material to the ethanol solution is 1g:50ml, extract it for 30 minutes at ultrasonic powers of 200W, 300W, 400W, 500W, and 600W; let the extract stand at room temperature, take the supernatant, filter it with a microporous filter membrane, and use HPLC to detect the content of curcuminoids. The results are shown in Table 2. Figure 3 .

[0054] like Figure 3As shown in the figure, since ultrasound can heat the water molecules in the cells and destroy the cell membrane structure, thereby accelerating the diffusion of solutes, the extraction rate of curcumin components gradually increases with the increase of ultrasonic power. When the ultrasonic power is 400W, the extraction efficiency is the highest. However, when the ultrasonic power is too high, the temperature will be too high, which not only destroys the stability of curcumin compounds, but also promotes their decomposition. Therefore, the extraction rate of curcumin components shows a trend of first increasing and then decreasing.

[0055] Example 4 Investigation of the Optimal Ultrasonic Time

[0056] Take fresh purple ginger medicinal material with a growth period of 3 years, wash it, cut it into slices with a thickness of 2mm, dry it in the shade at 25℃, grind it after the water content of the purple ginger is basically evaporated, add 90% ethanol solution, the mass volume ratio of the medicinal material to the ethanol solution is 1g:50ml, extract it for 15min, 30min, 45min, 60min, and 75min at an ultrasonic power of 400W; let the extract stand at room temperature, take the supernatant, filter it with a microporous filter membrane, and use HPLC to detect the content of curcuminoids. The results are shown in Table 1. Figure 4 .

[0057] like Figure 4 As shown in the figure, with the extension of ultrasonic time, the extraction rate of curcuminoid components showed an upward trend. This phenomenon was due to the fact that as the ultrasonic time increased, the thermal effect generated by ultrasound gradually increased, and the extraction efficiency was improved. When it reached 60 minutes, the extraction efficiency was the highest; then the extraction rate began to decline, which may be due to the fact that the ultrasonic time was too long and the temperature was too high, which accelerated the decomposition of curcuminoid compounds and led to a decrease in the extraction rate.

[0058] Example 5

[0059] Take fresh purple ginger medicinal material with a growth period of 3 years, wash it, cut it into slices with a thickness of 2 mm, dry it in the shade at 25°C, grind it after the water of the purple ginger is basically evaporated, add a 90% ethanol solution, the mass volume ratio of the medicinal material to the ethanol solution is 1g:50ml, extract it for 60 minutes at an ultrasonic power of 400W; after the extract is separated, the effective ingredients of the purple ginger are obtained.

[0060] Example 6

[0061] The same as Example 5, except that the growth period of the fresh purple ginger medicinal material is 5 years.

[0062] Comparative Example 1

[0063] The same as Example 5, except that the fresh purple ginger medicinal material is replaced with coral ginger.

[0064] Comparative Example 2

[0065] The same as Example 5, except that the fresh purple ginger medicinal material is replaced with turmeric.

[0066] Comparative Example 3

[0067] Take fresh purple ginger medicinal material with a growth period of 3 years, wash it, cut it into slices with a thickness of 2 mm, place it in an oven at 40°C, bake it for 72 hours, crush it, add a 90% ethanol solution with a mass volume ratio of the medicinal material to the ethanol solution of 1g:50ml, extract it for 60 minutes under an ultrasonic power of 400W; after separating the extract, the effective ingredients of purple ginger are obtained.

[0068] Comparative Example 4

[0069] Take fresh purple ginger medicinal material with a growth period of 5 years, wash it, cut it into slices with a thickness of 2 mm, place it in an oven at 40°C, bake it for 72 hours, crush it, add a 90% ethanol solution with a mass volume ratio of the medicinal material to the ethanol solution of 1g:50ml, extract it for 60 minutes under an ultrasonic power of 400W; after separation of the extract, the effective ingredients of purple ginger are obtained.

[0070] The extracts obtained in Examples 5-6 and Comparative Examples 1-4 were tested for the content of effective ingredients, and the results are shown in the following table:

[0071]

[0072] As can be seen from the above table, affected by the processing temperature, the content of curcumin components in the shade-drying treatment is significantly higher than that in the oven-drying treatment; as the purple ginger grows longer, the storage amount of curcumin and other compounds in the plant body increases, so under different processing conditions, the curcumin content of five-year-old purple ginger is higher than that of three-year-old purple ginger; the curcumin (CUR) in purple ginger is 3.8623 mg / g, which is significantly higher than that of coral ginger and turmeric, while the demethoxycurcumin (DMC) content in purple ginger is very close to that of coral ginger and turmeric.

[0073] Finally, it should be noted that the above description is only a preferred embodiment of the present invention. Under the guidance of the present invention, those skilled in the art can make a variety of similar expressions without violating the purpose and claims of the present invention, and such changes all fall within the protection scope of the present invention.

Claims

1. A method for preparing a high-content curcumin extract from purple ginger, a Dai medicine, characterized in that: The following steps are involved: S1. Take fresh purple ginger, wash it, and cut it into slices with a thickness of 2-5 mm; S2. Dry the slices until the moisture content is less than 10%; S3. The dried slices were crushed, 60-100% ethanol solution was added, and ultrasonic extraction was performed; S4. After separation, the extract is purified to obtain purple ginger extract.

2. The method for preparing a high-content curcumin extract from purple ginger of Dai medicine according to claim 1, characterized in that: The purple ginger medicinal material in S1 has a growth period of ≥3 years.

3. The method for preparing a high-content curcumin extract from purple ginger of Dai medicine according to claim 1, characterized in that: The drying method in S2 is room temperature drying.

4. A method for preparing a high-content curcumin extract from Dai medicine purple ginger according to any one of claims 1 to 3, characterized in that: The drying condition in S2 is: drying in the shade at 25° C. until the moisture content of the purple ginger is less than 10%.

5. The method for preparing a high-content curcumin extract from purple ginger, a Dai medicine, according to claim 1, characterized in that: In S3, the concentration of the ethanol solution is 60-95%, or 70-95%, or 80-95%, or 85-95%.

6. The method for preparing a high-content curcumin extract from purple ginger, a Dai medicine, according to claim 1, characterized in that: In S3, the mass volume ratio of the medicinal material to the ethanol solution is 1g: (30-70)ml.

7. The method for preparing a high-content curcumin extract from purple ginger of Dai medicine according to claim 6, characterized in that: In S3, the mass volume ratio of the medicinal material to the ethanol solution is 1g: (40-70) ml; 1g: (45-70) ml; 1g: (45-65) ml; 1g: (45-60) ml.

8. The method for preparing a high-content curcumin extract from purple ginger, a Dai medicine, according to claim 1, characterized in that: In S3, the ultrasonic extraction conditions are: ultrasonic power of 200W-600W, extraction for 10-80min.

9. The method for preparing a high-content curcumin extract from purple ginger, a Dai medicine, according to claim 8, characterized in that: In S3, the ultrasonic power is 300W-600W; or 400W-600W; or 350W-550W; or 350W-500W.

10. The method for preparing a high-content curcumin extract from purple ginger, a Dai medicine, according to claim 8, characterized in that: In S3, the ultrasonic time is 15-75 min; or 30-75 min; or 45-75 min; or 45-60 min.

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