Paenibacillus angularis culture device

Abstract

The invention discloses a Paenibacillus angularis culture device, and relates to the technical field of bacillus, the Paenibacillus angularis culture device comprises a fermentation tank, the top of the fermentation tank is fixedly connected with a feed pipe, the bottom of the fermentation tank is fixedly connected with a discharge pipe, and oxygen is injected into a closed space between a fixed cylinder and the fermentation tank through an oxygen injection pipe; oxygen is injected into the fermentation liquor through a plurality of pressure one-way valves and jet heads, so that the oxygen is in contact with more paenibacillus angularis and is absorbed by the paenibacillus angularis, meanwhile, the contact time of the oxygen and microorganisms can be prolonged, the oxygen and the microorganisms can be promoted to be in full contact, the overall growth rate of the aerobic paenibacillus angularis can be increased, and the yield of the aerobic paenibacillus angularis can be increased. Meanwhile, the problem that part of paenibacillus angularis at the bottom in the tank cannot be fully fused with oxygen due to the fact that oxygen is introduced from the upper part of the fermentation tank by an existing ventilation system is solved, so that harmful byproducts generated by anaerobic respiration of the paenibacillus angularis due to oxygen deficit can be avoided; furthermore, the yield and the quality of a target product can be ensured.

Description

Technical Field

[0001] The invention relates to the technical field of bacillus, in particular to a culture device for Bacillus aegypti. Background Art

[0002] Paenibacillus aegypti is a mesophilic, aerobic, spore-forming rod-shaped bacterium that is Gram-positive and widely found in nature. It is non-toxic and harmless to humans and animals and does not pollute the environment. It can produce a variety of secondary metabolites and enzymes during its growth. These substances give Paenibacillus aegypti important value in biological control, agricultural ecological environment improvement, soil improvement, and probiotic applications.

[0003] Since Bacillus is a strict aerobic microorganism, it needs a high dissolved oxygen to grow well. Although the existing culture device is equipped with a ventilation system, the existing ventilation system usually introduces oxygen from the top of the fermenter. This method may prevent oxygen from effectively flowing to the bottom of the fermenter cavity, resulting in it being unable to fully merge with the Bacillus aegypti located at the bottom of the fermenter cavity. As a result, the growth of Bacillus aegypti in some areas of the fermenter may be restricted due to lack of oxygen, resulting in a decrease in the overall growth rate. It may also cause Bacillus aegypti to adjust its metabolic pathway, perform anaerobic respiration or produce byproducts that are harmful to cells, which not only affects the yield and quality of the target product, but may also cause damage to the cells themselves. At the same time, the existing stirring method usually uses a stirrer installed in the fermenter to rotate to drive the fermentation liquid to move. Due to factors such as the large volume and high viscosity of the fermentation liquid, this stirring method generally can only make the fermentation liquid flow radially, so that the fermentation liquid and the bacteria in the fermenter can only move along the rotation direction of the stirrer. It may not be possible to fully mix the nutrients in the fermentation liquid, resulting in inconsistent nutrient concentrations in the fermentation liquid and affecting the growth rate and metabolic activity of the bacteria. Summary of the invention

[0004] In view of this, and in view of the deficiencies of the prior art, the present invention provides a culture device for Bacillus eggei, so as to solve the problem in the prior art that oxygen cannot effectively flow to the bottom of the fermentation tank, causing the overall growth rate of Bacillus eggei in some areas to decrease due to lack of oxygen.

[0005] To achieve the above object, the present invention provides the following technical solution: a Paenibacillus aegypti culture device, comprising a fermentation tank, a feed pipe is fixedly connected to the top of the fermentation tank, a discharge pipe is fixedly connected to the bottom of the fermentation tank, and a tank cover is hinged on the feed pipe and the discharge pipe;

[0006] The outer surface of the fermentation tank is rotatably connected with a fixed cylinder, and the fixed cylinder and the fermentation tank are sealed, the outer surface of the fixed cylinder is fixedly connected with an oxygen injection pipe, and the oxygen injection pipe is fixedly connected with a solenoid valve, and the outer surface of the fermentation tank is equidistantly fixedly connected with a plurality of pressure check valves, one end of each of the plurality of pressure check valves is fixedly connected with an injection head, and the injection head is located inside the fermentation tank, and the bottom of the fixed cylinder is fixedly connected with a support frame;

[0007] The fermentation tank is provided with a stirring mechanism, the feed pipe and the discharge pipe are provided with an early warning mechanism, and the fermentation tank is provided with a defoaming mechanism.

[0008] Preferably, the stirring mechanism includes a driving motor, which is fixedly connected to the middle of the supporting frame, and the output shaft of the driving motor is fixedly connected to the outer wall of the bottom of the fermentation tank. The inner wall of the fermentation tank is equidistantly fixedly connected with protrusion plates, the top of the fixed cylinder is fixedly connected with a connecting frame, and the upper part of the connecting frame is fixedly connected with an undulating plate.

[0009] Preferably, there are no less than two connecting rods equidistantly slidably connected to the top of the fermentation tank, and sliders are fixedly connected to the tops of the two connecting rods, and the sliders are in contact with the protruding plates. The sliders are fixedly connected to the opposite surfaces of the fermentation tank top with telescopic springs, and the telescopic springs are sleeved on the outer surfaces of the connecting rods.

[0010] Preferably, the bottom ends of the two connecting rods are fixedly connected to a pressing plate, both sides of the pressing plate are in contact with the inner wall of the fermentation tank, both sides of the pressing plate are rolled inward, and the rolled parts are located at the top of the pressing plate.

[0011] Preferably, the early warning mechanism includes a mounting sleeve, which is fixedly connected to the outer surface of one end of the feed pipe and the discharge pipe away from the fermentation tank, a rubber airbag is fixedly installed on the inner surface of the mounting sleeve, a sealing cylinder is fixedly connected to the outer surface of the rubber airbag, and one end of the sealing cylinder is located outside the mounting sleeve.

[0012] Preferably, a piston head is slidably connected inside the sealing cylinder, a trigger block is fixedly connected to a side of the piston head away from the rubber airbag, and a touch switch is fixedly connected to an inner wall of a side of the sealing cylinder away from the rubber airbag.

[0013] Preferably, a buzzer alarm is fixedly connected to the upper part of the connecting frame, the trigger block is electrically connected to the touch switch and the opening and closing of the buzzer alarm is controlled by the controller, and the trigger block is electrically connected to the touch switch and the opening and closing of the solenoid valve is controlled by the controller.

[0014] Preferably, the defoaming mechanism includes a defoaming sleeve, and a plurality of the defoaming sleeves are provided, and the plurality of defoaming sleeves are slidably connected to the outer surface of the connecting rod, the upper and lower ends of the defoaming sleeve are rotatably connected to a support rod, and the support rod is fixedly connected to the inner wall of the fermentation tank.

[0015] Preferably, defoaming rods are equidistantly fixedly installed on the outer surface of the defoaming sleeve, a spiral groove is opened on the inner surface of the defoaming sleeve, and a limiting ball is fixedly connected to the outer surface of the connecting rod, and the limiting ball is adapted to the spiral groove.

[0016] Compared with the prior art, the present invention provides a device for cultivating Paenibacillus aegypti, which has the following beneficial effects:

[0017] Oxygen is injected into the closed space between the fixed cylinder and the fermentation tank through an oxygen injection pipe, and oxygen is injected into the fermentation liquid through multiple pressure one-way valves and air jets, so that oxygen can contact with more Paenibacillus aegypti and be absorbed by them, and at the same time, the contact time between oxygen and microorganisms can be increased, so that the two can be fully in contact, thereby increasing the overall growth rate of aerobic Paenibacillus aegypti, and solving the problem that the existing ventilation system introduces oxygen from the upper position of the fermentation tank, so that part of Paenibacillus aegypti at the bottom of the tank cannot be fully integrated with oxygen, thereby preventing Paenibacillus aegypti from performing anaerobic respiration due to lack of oxygen to produce harmful byproducts, thereby ensuring the yield and quality of the target product.

[0018] By starting the servo motor, the protruding plate in the fermentation tank moves the Bacillus aegypti and the fermentation liquid, and then drives the sliders on the two connecting rods to slide up and down along the undulating plates. At the same time, under the action of the telescopic spring, the pressing plate at the bottom of the connecting rod can change the upper and lower positions of the fermentation liquid in the fermentation tank, so that the fermentation liquid can be stirred horizontally and vertically. The fermentation liquid can make a circular motion in the horizontal direction and reciprocate in the vertical direction at the same time, thereby enhancing the turbulence and shear force in the fermentation liquid, promoting the transfer and mixing of substances, and stirring the fermentation liquid with a larger volume and a higher viscosity, so as to fully mix the nutrients in the fermentation liquid.

[0019] When leakage occurs between the feed pipe or the discharge pipe and the tank cover, the high-pressure gas leaked in the fermentation tank impacts and squeezes the rubber airbag, causing the inside of the rubber airbag to dent and discharge the internal air into the sealing cylinder, causing the piston head to push the trigger block to contact the touch switch, connecting the circuit of the buzzer alarm and the solenoid valve, and under the action of the controller, the buzzer alarm is controlled to start working and emit a buzzing sound, thereby attracting the attention of the operator, so that the operator can take corresponding measures in time to prevent external bacteria from entering the fermentation tank and contaminating Bacillus aegypti, thereby ensuring its normal growth and fermentation effect, and at the same time prompting the solenoid valve to close, preventing oxygen from being injected into the fermentation tank again when leakage occurs in the feed pipe or the discharge pipe, thereby increasing the leakage at the leakage position.

[0020] When the connecting rod slides up and down, the limiting ball on the connecting rod slides up and down along the inside of the spiral groove, causing the defoaming sleeve and the multiple defoaming rods thereon to rotate continuously, thereby breaking the foam on the top of the fermentation liquid, improving the gas exchange efficiency between oxygen and the fermentation liquid, and ensuring that Bacillus aegypti obtains sufficient oxygen for aerobic respiration, which is beneficial to the growth and metabolic activities of the bacteria, thereby ensuring the stability of the fermentation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the cross-section structure of the fixed cylinder in the present invention;

[0023] Figure 3 It is a schematic diagram of the cutaway structure of the fermentation tank in the present invention;

[0024] Figure 4 It is an exploded view of the structure inside the fermentation tank of the present invention;

[0025] Figure 5 It is a structural schematic diagram of the can lid in the open state in the present invention;

[0026] Figure 6 It is a structural explosion diagram of the early warning mechanism in the present invention;

[0027] Figure 7 For the present invention Figure 6 A magnified view of the structure at center A;

[0028] Figure 8 It is a schematic diagram of the cross-section structure of the defoaming sleeve in the present invention;

[0029] Fig. 9 It is a structural explosion diagram of the defoaming sleeve in the present invention;

[0030] Fig.10It is a structural explosion diagram of the defoaming sleeve in the present invention in a cut-away and enlarged state.

[0031] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0032] 1. Fermentation tank; 101. Feed pipe; 102. Discharge pipe; 103. Tank cover; 2. Fixed cylinder; 201. Oxygen injection pipe; 202. Pressure check valve; 203. Injection head; 204. Solenoid valve; 205. Support frame;

[0033] Stirring mechanism: 3, driving motor; 301, protruding plate; 302, connecting frame; 303, undulating plate; 304, connecting rod; 305, slider; 306, telescopic spring; 307, pressing plate;

[0034] Early warning mechanism: 4, installation sleeve; 401, rubber airbag; 402, sealing tube; 403, piston head; 404, trigger block; 405, touch switch; 406, buzzer alarm;

[0035] Defoaming mechanism: 5, defoaming sleeve; 501, support rod; 502, defoaming rod; 503, spiral groove; 504, limiting ball. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] The present invention is further described in detail below based on the accompanying drawings and embodiments.

[0038] Embodiment 1

[0039] Please refer to Figures 1 to 4As shown, in order to solve the problems mentioned in the technical solution, the embodiment of the present application provides a culture device for Paenibacillus aegypti, including a fermenter 1, a feed pipe 101 is fixedly connected to the top of the fermenter 1, a discharge pipe 102 is fixedly connected to the bottom of the fermenter 1, and a tank cover 103 is hinged on the feed pipe 101 and the discharge pipe 102, wherein a sealing ring is arranged between the tank cover 103 and the feed pipe 101 and the discharge pipe 102 for sealing, so as to prevent leakage of the fermenter 1 and cause external bacteria to enter the fermenter 1. The outer surface of the fermenter 1 is rotatably connected with a fixed cylinder 2, and the fixed cylinder 2 and the fermenter 1 are sealed, so that oxygen is stored through the closed space between the two, the outer surface of the fixed cylinder 2 is fixedly connected with an oxygen injection pipe 201, and the oxygen injection pipe 201 is fixedly connected with a solenoid valve 204, and the outer surface of the fermenter 1 is equidistantly fixedly connected with a plurality of pressure check valves 202, and the plurality of pressure check valves 202 are all located at the lower part of the fermenter 1, so that oxygen can fully contact with the microorganisms. One end of each of the multiple pressure check valves 202 is fixedly connected to a spray head 203 , and the spray head 203 is located inside the fermentation tank 1 , and a support frame 205 is fixedly connected to the bottom of the fixed cylinder 2 .

[0040] Working process: When in use, the worker first injects a certain amount of liquid culture medium rich in nutrients into the fermentation tank 1 from the feed pipe 101, and then injects a certain amount of culture medium of Bacillus aegypti from the feed pipe 101 into the fermentation tank 1, so that the two are mixed with each other. Subsequently, during the inoculation and fermentation process, oxygen is injected into the closed space between the fixed cylinder 2 and the fermentation tank 1 through the oxygen injection pipe 201. At this time, under the action of multiple pressure check valves 202, the internal air pressure inside the sealed space increases due to the injection of oxygen, and the pressure difference between the sealed space and the fermentation tank 1 gradually increases. When the internal air pressure value of the sealed space exceeds the maximum threshold value set by the pressure check valve 202, the oxygen is injected from the multiple pressure check valves 202 into the fermentation tank 1 through the pressure difference between the sealed space and the fermentation tank 1. The oxygen is discharged into the fermentation tank 1, and then sprayed to the lower part of the inner cavity of the fermentation tank 1 by the nozzle 203, so that the oxygen with a relatively high pressure is injected into the fermentation liquid. Since the density of oxygen is much smaller than the density of the fermentation liquid, the oxygen floats upward, so that the oxygen can contact with more Paenibacillus aegypti and be absorbed by it. At the same time, the contact time between oxygen and microorganisms can be increased, so that the two can be fully in contact, thereby increasing the overall growth rate of aerobic Paenibacillus aegypti, and solving the problem that the existing ventilation system introduces oxygen from the upper part of the fermentation tank 1, so that part of Paenibacillus aegypti at the bottom of the tank cannot be fully integrated with oxygen, thereby avoiding the Paenibacillus aegypti from performing anaerobic respiration due to lack of oxygen to produce harmful by-products, thereby ensuring the yield and quality of the target product.

[0041] Embodiment 2

[0042] Please refer to Figure 3 , Figure 4 as well as Fig. 9 As shown, the fermentation tank 1 is provided with a stirring mechanism for avoiding damage to microbial cells, and the stirring mechanism includes a driving motor 3, which is fixedly connected to the middle of the support frame 205, and the output shaft of the driving motor 3 is fixedly connected to the outer wall of the bottom of the fermentation tank 1. The inner wall of the fermentation tank 1 is fixedly connected with protrusion plates 301 at equal intervals, and the protrusion plates 301 are generally arc-shaped to increase the contact area with the microorganisms. The top of the fixed cylinder 2 is fixedly connected with a connecting frame 302, and the upper part of the connecting frame 302 is fixedly connected with an undulating plate 303, and the cross-section of the undulating plate 303 is circular, and the side of the undulating plate 303 facing the fixed cylinder 2 is wavy. There are no less than two connecting rods 304 equidistantly slidably connected to the top of the fermenter 1, and the two connecting rods 304 extend to the outside of the fermenter 1, and sliders 305 are fixedly connected to the tops of the multiple connecting rods 304, and the sliders 305 are in contact with the protruding plate 301, and the sliders 305 are fixedly connected to the opposite surfaces of the top of the fermenter 1 with telescopic springs 306, and the telescopic springs 306 are sleeved on the outer surfaces of the connecting rods 304, and the bottom ends of the two connecting rods 304 are commonly fixedly connected with a pressing plate 307, and both sides of the pressing plate 307 are in contact with the inner wall of the fermenter 1, and both sides of the pressing plate 307 are inwardly rolled up, and the rolled-up parts are located at the top of the pressing plate 307, so that some microorganisms can be pulled up, thereby accelerating the mixing speed of Bacillus aegypti and nutrients.

[0043] Working process: Start the servo motor so that the output shaft of the servo motor drives the fermentation tank 1 to rotate along the inside of the fixed cylinder 2, and the arc-shaped protrusion plate 301 in the fermentation tank 1 moves the Bacillus aegypti and the fermentation liquid near the tank wall of the fermentation tank 1 during the rotation process. The shear force generated by this method is small, and thus will not cause damage to the microbial cells, thereby ensuring their growth and metabolic activity. At the same time, during the rotation of the fermentation tank 1, it drives the two connecting rods 304 to rotate and move, and makes the slider 305 on the connecting rod 304 slide along the surface of the undulating plate 303. When the slider 305 slides from the highest point of the undulating plate 303 to the lowest point, the connecting rod 304 is squeezed and slides vertically downward, so that the distance between the slider 305 and the top of the fermentation tank 1 is shortened, thereby squeezing the telescopic spring 306 to contract, and the pressing plate 307 at the bottom of the connecting rod 304 slides vertically downward, causing the pressing plate 307 to extend into the fermentation liquid. In this process In the process, the bottom of the pressing plate 307 squeezes the fermentation liquid, causing the fermentation liquid below to flow to the periphery, so that a short gap appears in the space above the pressing plate 307, and then the two sides of the pressing plate 307 in a rolled shape guide the surrounding fermentation liquid to the top position of the pressing plate 307, thereby the fermentation liquid that was originally in a static state can be continuously changed. When the slider 305 slides from the lowest point of the undulating plate 303 to the highest point, the distance between the slider 305 and the top of the fermentation tank 1 increases, so that the telescopic spring 306 gradually extends and pushes the slider 305 to fit on the undulating plate 3 03 surface, and at the same time, the connecting rod 304 slides vertically upward, thereby extracting the pressing plate 307 from the fermentation liquid. In this process, the pressing plate 307 lifts the fermentation liquid on the top thereof upward, and makes the lifted fermentation liquid flow out of the pressing plate 307 from the gaps between the two sides of the pressing plate 307 and the fermentation tank 1. In this way, the position of the fermentation liquid can also be changed, thereby promoting the fermentation liquid to flow in the up and down directions. In the process of injecting oxygen from the bottom of the fermentation tank 1 into the interior thereof, the pressing plate 307 can be moved up and down to lift the fermentation liquid. The fusion effect of the fermentation liquid and oxygen is high. At the same time, the fermentation tank 1 drives the multiple protrusion plates 301 to rotate synchronously, so that the fermentation liquid can be stirred in both horizontal and vertical directions, so that the fermentation liquid can make a circular motion in the horizontal direction and reciprocate in the vertical direction at the same time, thereby enhancing the turbulence and shear force in the fermentation liquid, promoting the transfer and mixing of substances, and stirring the fermentation liquid with a larger volume and a higher viscosity, so as to promote the full mixing of nutrients in the fermentation liquid, thereby ensuring the growth rate and metabolic activity of Bacillus aegypti.

[0044] Embodiment 3

[0045] Please refer to Figures 5 to 7As shown, the feed pipe 101 and the discharge pipe 102 are provided with an early warning mechanism for detecting whether the fermentation tank 1 has a leakage problem, and the early warning mechanism includes a mounting sleeve 4, which is fixedly connected to the outer surface of the feed pipe 101 and the discharge pipe 102 at one end away from the fermentation tank 1, and the inner diameter of the mounting sleeve 4 is larger than the outer diameter of the tank cover 103, thereby ensuring that the tank cover 103 can fit on the feed pipe 101 and the discharge pipe 102. A rubber airbag 401 is fixedly installed on the inner surface of the mounting sleeve 4, and the rubber airbag 401 is tightly fitted and covers the intersection of the feed pipe 101, the discharge pipe 102 and the tank cover 103. The outer surface of the rubber airbag 401 is fixedly connected with a sealing cylinder 402, and the rubber airbag 401 is connected with the sealing cylinder 402. One end of the sealing cylinder 402 is located outside the mounting sleeve 4. The piston head 403 is slidably connected inside the sealing cylinder 402. The side of the piston head 403 away from the rubber airbag 401 is fixedly connected with a trigger block 404. The inner wall of the side of the sealing cylinder 402 away from the rubber airbag 401 is fixedly connected with a touch switch 405. A buzzer alarm 406 is fixedly connected to the upper part of the connecting frame 302. The trigger block 404 and the touch switch 405 are electrically connected and the opening and closing of the buzzer alarm 406 are controlled by the controller. The trigger block 404 and the touch switch 405 are electrically connected and the opening and closing of the solenoid valve 204 are controlled by the controller.

[0046] Working process: when leakage occurs between the feed pipe 101 or the discharge pipe 102 and the tank cover 103, the gas inside the fermentation tank 1 flows out from the intersection between the two. The outflowing gas impacts the surface of the rubber airbag 401 due to its high pressure, causing the inner circle of the rubber airbag 401 that fits the tank cover 103 to deform, and under the restriction of the installation sleeve 4, the rubber airbag 401 cannot expand. At this time, its internal space is reduced and the air pressure is increased, which prompts the air inside the rubber airbag 401 to flow into the sealing tube 402. The air flowing into it squeezes the piston head 403 to slide toward the other end of the sealing tube 402, and drives the trigger block 404 thereon to slide synchronously until the trigger block 404 contacts the touch switch 405, which can quickly attract the attention of the operator, so that the operator can take corresponding measures in time to prevent external bacteria from entering the fermentation tank 1 and contaminating the egg white. Bacillus, thereby ensuring its normal growth and fermentation effect. When the staff solves the leakage problem, the rubber airbag 401 is no longer impacted by the pressure of the leaking gas, so that the rubber airbag 401 loses the external force squeezing and returns to its original state. At this time, the space in the sealing tube 402 increases and the air pressure decreases, prompting the air inside the sealing tube 402 to flow into the rubber airbag 401, causing the piston head 403 to drive the trigger block 404 to disengage from the touch switch 405, thereby the circuit of the buzzer alarm 406 is disconnected, and under the action of the controller, the buzzer alarm 406 is controlled to stop the buzzing sound, and at the same time, the circuit of the solenoid valve 204 is connected through the contact between the trigger block 404 and the touch switch 405, and under the action of the controller, the valve of the solenoid valve 204 is closed to prevent oxygen from being injected into the fermentation tank 1 again when leakage occurs in the feed pipe 101 or the discharge pipe 102, thereby increasing the leakage amount at the leakage position.

[0047] Embodiment 4

[0048] Please refer to Figure 3 , Figure 8 as well as Fig. 9As shown, the fermenter 1 is provided with a defoaming mechanism for removing the foam generated inside the fermenter 1, and the defoaming mechanism includes a defoaming sleeve 5, and the defoaming sleeve 5 is provided in plurality, and the plurality of defoaming sleeves 5 are all slidably connected to the outer surface of the connecting rod 304, and the upper and lower ends of the defoaming sleeve 5 are rotatably connected to the support rod 501, and the support rod 501 is fixedly connected to the inner wall of the fermenter 1, wherein the connecting rod 304 can slide up and down along the inner surface of the defoaming sleeve 5, and the defoaming sleeve 5 can rotate on the support rod 501. Defoaming rods 502 are fixedly installed equidistantly on the outer surface of the defoaming sleeve 5, and the defoaming rods 502 are located above and adjacent to the liquid level after the quantitative liquid culture medium and Paenibacillus aegyptiaca are mixed, thereby ensuring that the defoaming rods 502 are located in the foam generated during the fermentation process. A spiral groove 503 is provided on the inner surface of the defoaming sleeve 5, wherein the spiral groove 503 is composed of two parts, which are symmetrical to each other and have opposite spiral directions. At the same time, the spiral groove 503 is a closed-loop design as a whole, and its ends are connected; a limiting ball 504 is fixedly connected to the outer surface of the connecting rod 304, and the limiting ball 504 is adapted to the spiral groove 503.

[0049] Working process: when the connecting rod 304 slides vertically downward, the limiting ball 504 on the connecting rod 304 moves synchronously therewith, and the limiting ball 504 slides from the highest point inside to the lowest point along the notch on one side of the spiral groove 503, so that the limiting ball 504 squeezes the spiral groove 503 downward, prompting the defoaming sleeve 5 to gradually rotate, and the multiple defoaming rods 502 on the outer surface of the defoaming sleeve 5 also rotate synchronously. Similarly, when the connecting rod 304 slides vertically upward, the limiting ball 504 slides from the lowest point inside to the highest point along the notch on the other side of the spiral groove 503, so that the limiting ball 504 squeezes the spiral groove 503 upward, prompting the defoaming sleeve 5 to rotate in the same direction as the above process, and then the multiple defoaming rods 502 can be prompted to rotate continuously, thereby breaking the foam at the top of the fermentation liquid, and improving the gas exchange efficiency between oxygen and the fermentation liquid, ensuring that Bacillus aegypti obtains sufficient oxygen for aerobic respiration, which is beneficial to the growth and metabolic activities of the bacteria, thereby ensuring the stability of the fermentation process.

[0050] The above is a detailed introduction to the present application. Specific examples are used in this article to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method of the present application and its core idea. At the same time, for general technical personnel in this field, according to the idea of ​​the present application, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A device for cultivating Paenibacillus aegypti, characterized in that: The fermentation tank comprises a fermentation tank (1), wherein a feed pipe (101) is fixedly connected to the top of the fermentation tank (1), a discharge pipe (102) is fixedly connected to the bottom of the fermentation tank (1), and a tank cover (103) is hingedly connected to the feed pipe (101) and the discharge pipe (102); The outer surface of the fermentation tank (1) is rotatably connected to a fixed cylinder (2), and the fixed cylinder (2) and the fermentation tank (1) are sealed. The outer surface of the fixed cylinder (2) is fixedly connected to an oxygen injection pipe (201), and the oxygen injection pipe (201) is fixedly connected to a solenoid valve (204). The outer surface of the fermentation tank (1) is equidistantly fixedly connected to a plurality of pressure check valves (202), one end of each of the plurality of pressure check valves (202) is fixedly connected to an injection head (203), and the injection head (203) is located inside the fermentation tank (1). The bottom of the fixed cylinder (2) is fixedly connected to a support frame (205); The fermentation tank (1) is provided with a stirring mechanism, the feed pipe (101) and the discharge pipe (102) are provided with an early warning mechanism, and a defoaming mechanism is provided inside the fermentation tank (1).

2. The device for cultivating Paenibacillus gegenes according to claim 1, characterized in that: The stirring mechanism comprises a driving motor (3), the driving motor (3) is fixedly connected to the middle part of the support frame (205), the output shaft of the driving motor (3) is fixedly connected to the outer wall of the bottom of the fermentation tank (1), the inner wall of the fermentation tank (1) is fixedly connected with protrusion plates (301) at equal intervals, the top of the fixed cylinder (2) is fixedly connected with a connecting frame (302), and the upper part of the connecting frame (302) is fixedly connected with an undulating plate (303).

3. The device for cultivating Paenibacillus gegenes according to claim 2, characterized in that: The top of the fermentation tank (1) is equidistantly and slidably connected to at least two connecting rods (304), the tops of the two connecting rods (304) are fixedly connected with sliders (305), and the sliders (305) are in contact with the protruding plate (301), and the sliders (305) are fixedly connected with telescopic springs (306) on the opposite surfaces of the top of the fermentation tank (1), and the telescopic springs (306) are sleeved on the outer surfaces of the connecting rods (304).

4. The device for cultivating Paenibacillus gegenes according to claim 3, characterized in that: The bottom ends of the two connecting rods (304) are fixedly connected to a pressing plate (307), and both sides of the pressing plate (307) are in contact with the inner wall of the fermentation tank (1). Both sides of the pressing plate (307) are rolled inward, and the rolled parts are located at the top of the pressing plate (307).

5. The device for cultivating Paenibacillus aegypti according to claim 2, characterized in that: The early warning mechanism comprises a mounting sleeve (4), wherein the mounting sleeve (4) is fixedly connected to the outer surface of one end of the feed pipe (101) and the discharge pipe (102) away from the fermentation tank (1), a rubber airbag (401) is fixedly installed on the inner surface of the mounting sleeve (4), and a sealing cylinder (402) is fixedly connected to the outer surface of the rubber airbag (401), and one end of the sealing cylinder (402) is located outside the mounting sleeve (4).

6. The device for cultivating Paenibacillus aegypti according to claim 5, characterized in that: The sealing cylinder (402) is slidably connected to a piston head (403) inside, a side of the piston head (403) away from the rubber airbag (401) is fixedly connected to a trigger block (404), and an inner wall of a side of the sealing cylinder (402) away from the rubber airbag (401) is fixedly connected to a touch switch (405).

7. The device for cultivating Paenibacillus aegypti according to claim 6, characterized in that: A buzzer alarm (406) is fixedly connected to the upper part of the connecting frame (302); the trigger block (404) is electrically connected to the touch switch (405) and the opening and closing of the buzzer alarm (406) are controlled by a controller; and the trigger block (404) is electrically connected to the touch switch (405) and the opening and closing of the solenoid valve (204) are controlled by a controller.

8. The device for cultivating Paenibacillus aegypti according to claim 3, characterized in that: The defoaming mechanism comprises a defoaming sleeve (5), and the defoaming sleeve (5) is provided in plurality, and the plurality of defoaming sleeves (5) are all slidably connected to the outer surface of the connecting rod (304), and the upper and lower ends of the defoaming sleeve (5) are rotatably connected to a support rod (501), and the support rod (501) is fixedly connected to the inner wall of the fermentation tank (1).

9. The device for cultivating Paenibacillus aegypti according to claim 8, characterized in that: The outer surface of the defoaming sleeve (5) is fixedly provided with defoaming rods (502) at equal intervals, the inner surface of the defoaming sleeve (5) is provided with a spiral groove (503), and the outer surface of the connecting rod (304) is fixedly connected with a limiting ball (504), and the limiting ball (504) is adapted to the spiral groove (503).

Images