A dual-bioreactor approach to improve nutrient utilization

Abstract

The present invention discloses a dimeric biological method for improving nutrient utilization, and belongs to the field of biological preparation technology. The dimeric biological method for improving nutrient utilization includes the following steps: step 1: testing the nutrients in the soil, and then sowing crops; step 2: applying dimeric chelates and compound fertilizers in the seedling stage, jointing stage and fruiting stage of the crops; step 3: harvesting the crops according to their growth conditions. This method can effectively improve the utilization of nutrients and increase the yield of crops.

Description

Technical Field

[0001] The invention belongs to the technical field of biological preparation, and in particular relates to a dipolymer biological method capable of improving nutrient utilization. Background Art

[0002] As an innovative biotechnology, dimeric biotechnology has shown great potential in improving nutrient utilization. The core of this technology is to use dimeric compounds, which are high-molecular compounds formed by the polymerization of two identical or similar molecules in a specific way. These compounds usually have unique physical and chemical properties and high stability. In the field of biotechnology, dimeric compounds have been widely studied and applied due to their unique combination and properties, especially in improving nutrient utilization, playing a vital role.

[0003] In agricultural production, nutrient utilization rate is an important indicator to measure crop growth efficiency and soil fertility. However, traditional fertilization methods often have problems such as nutrient loss and low utilization rate, which not only wastes resources but also pollutes the environment. Dimer compounds can slowly release nutrients according to the growth needs of crops, so that nutrients can be more fully utilized during the growth process of crops. The precise nutrient release method not only improves nutrient utilization rate, but also reduces the number and amount of fertilization, reducing production costs and environmental pressure.

[0004] CN108503462A discloses a polymerized amino acid fertilizer for improving nutrient utilization and a preparation method thereof, wherein the fertilizer is made of urea, diammonium phosphate, potassium sulfate, polyglutamic acid, synergist, oyster powder, and livestock manure biogas slurry according to a certain weight ratio. The fertilizer is added with polymerized amino acids and synergist, and the two act synergistically to significantly increase the content of soil nitrate nitrogen, available phosphorus, and available potassium, and can ensure the normal growth of crops throughout the growth period; significantly increase the accumulation of crop dry matter, and at the same time increase the biomass of leaves and rhizomes, promote photosynthesis and nutrient absorption and transfer, and improve the utilization efficiency of nitrogen, phosphorus, and potassium. However, there is still room for improvement in the utilization of nutrients through this method. Summary of the invention

[0005] The object of the present invention is to provide a dimeric biomass method capable of improving nutrient utilization, so as to solve the technical problems of poor nutrient utilization and crop yield in the prior art.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] The present invention provides a dimeric biomass method for improving nutrient utilization, comprising the following steps:

[0008] Step 1: Test the soil for nutrients and then plant the crops;

[0009] Step 2: Apply double polychelate and compound fertilizers during the seedling, jointing and fruiting stages of crops;

[0010] Step 3: Harvest the crops according to their growth conditions.

[0011] Preferably, in step 1, the crops are one or more combinations of wheat, corn, soybeans and cotton.

[0012] Preferably, the method for preparing the dimeric chelate comprises the following steps:

[0013] Q1: After pre-treatment by heating the rice straw in a sodium hydroxide solution, the rice straw is washed, dried, and ground. The powder is added to a hydrogen peroxide solution under heating conditions, sheared, washed, filtered, and dispersed to obtain a suspension.

[0014] Q2: Dissolve polyacrylamide, potassium persulfate and N,N-methylenebispropylene in deionized water, stir vigorously until completely dissolved, add the suspension, stir evenly, add tetramethylethylenediamine, heat and polymerize to obtain a gel carrier;

[0015] Q3: Add β-glucosidase and protease to the thiamine pyrophosphate solution, mix well, add a gel carrier, solidify, and dry to obtain a dimeric chelate.

[0016] In the above process, rice straw is used as raw material and a suspension is prepared by hydrogen peroxide oxidation. Hydrogen peroxide will destroy the amorphous region of cellulose and oxidize some hydroxyl groups in cellulose to carboxyl groups, weakening the hydrogen bonding between cellulose, thereby dissociating cellulose from microfibrils by mechanical shearing to obtain a suspension. Subsequently, polyacrylamide is used as a monomer, potassium persulfate is used as an initiator, and N,N-methylenebispropylene is used as a cross-linking agent to prepare a gel carrier. Finally, thiamine pyrophosphate is used as a coenzyme to promote the interaction between β-glucosidase and protease, and the suspension is solidified with a gel carrier to obtain a dimerized chelate.

[0017] Preferably, in Q1, the amount ratio of rice straw to sodium hydroxide solution is (10-20) g: (100-150) mL, the volume fraction of sodium hydroxide solution is 10 vt%, the heating pretreatment temperature is 80-90°C, the treatment time is 8-12 h, washed with deionized water, dried at 60-80°C for 10-12 h, ground to a particle size of 200-400 mesh, the amount ratio of powder to hydrogen peroxide solution is (1-3) g: (100-200) mL, the temperature under heating conditions is 80-85°C, the volume fraction of hydrogen peroxide solution is 30 vt%, the treatment time is 6-12 h, the shear speed is 3000-4000 rpm, the treatment time is 3-5 h, dispersed in deionized water, and the concentration of the suspension is 0.1 g / mL.

[0018] Preferably, in Q2, the dosage ratio of polyacrylamide, potassium persulfate, N,N-methylenebispropylene, deionized water, suspension and tetramethylethylenediamine is (5-7.5) g: (0.04-0.06) g: (0.003-0.0045) g: (12-20) mL: (8-12) mL: (20-25) μL, the vigorous stirring time is 1-2 h, the heating polymerization temperature is 60-80 ° C, and the time is 30-45 min.

[0019] Preferably, in Q3, the dosage ratio of ammonium pyrophosphate solution, β-glucosidase, protease and gel carrier is (2-5) mL: (0.02-0.03) g: (0.01-0.02) g: (4-6) g, and the mixing time is 40-60 min.

[0020] Preferably, the method for preparing the compound fertilizer comprises the following steps:

[0021] S1: adding methyl 2,4-dioxo-4-thiophene-2-ylbutyrate to methanol, adding 2-hydrazinepyridine and hydrochloric acid after stirring, heating to reflux, cooling, spin drying, dissolving, washing, neutralizing, extracting, drying, and spin drying to obtain intermediate 1;

[0022] S2: adding intermediate 1 to a container filled with anhydrous ethanol, adding hydrazine hydrate, magnetically stirring, suction filtering, washing, and drying to obtain intermediate 2, dissolving intermediate 2 and o-carboxybenzaldehyde in anhydrous ethanol, stirring at room temperature, suction filtering, washing, and drying to obtain a carboxylic acid derivative;

[0023] S3: Mix carboxylic acid derivatives, urea, superphosphate and wood ash to obtain compound fertilizer.

[0024] In the above process, the synthesis reaction formula of the carboxylic acid derivative is as follows:

[0025]

[0026] The results of mass spectrometry analysis of intermediate 1 were: m / z: 285.06 (100.0%), 286.06 (16.1%), 287.05(4.5%), 287.06 (1.8%), 286.05 (1.1%); the results of mass spectrometry analysis of intermediate 2 were: m / z: 285.07(100.0%), 286.07 (16.9%), 287.06 (4.5%); the results of mass spectrometry analysis of carboxylic acid derivatives were: m / z:417.09 (100.0%), 418.09 (25.5%), 419.09 (5.8%), 419.10 (2.5%), 420.09 (1.1%).

[0027] Preferably, in S1, the amount ratio of methyl 2,4-dioxo-4-thiophene-2-ylbutyrate, methanol, 2-hydrazinepyridine and hydrochloric acid is (1.78-2.21) g: (20-25) mL: (0.91-1.02) g: (8-12) mL, the heating reflux temperature is 80-90°C, the reflux time is 5-7h, and it is added to chloroform for dissolution, washed with deionized water, neutralized with sodium bicarbonate solution, and dried with sodium sulfite.

[0028] Preferably, in S2, the amount ratio of intermediate 1, anhydrous ethanol and hydrazine hydrate is (2.15-3.42) g: (25-30) mL: (5-7.5) mL, the magnetic stirring time is 8-10 h, and the mixture is washed with cold ethanol. The amount ratio of intermediate 2, o-carboxybenzaldehyde and anhydrous ethanol is (0.057-0.074) g: (0.031-0.036) g: (5-8) mL, the stirring time at room temperature is 6-10 h, and the mixture is washed with cold ethanol. In S3, the amount ratio of carboxylic acid derivative, urea, superphosphate and wood ash is (80-100) g: (1000-1500) g: (900-1400) g: (1000-1200) g, and the mixing time is 2-4 h.

[0029] Preferably, in step 2, the application is by strip application or broadcast application.

[0030] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0031] The present invention applies the prepared double polymer chelate and compound fertilizer to the growth process of plants. The double polymer chelate has excellent stability, can significantly reduce the loss and fixation of nutrients in the soil, and the damage caused by hydrolysis, oxidation and microbial degradation, and effectively prolong the effective time of nutrients. Its chelated form not only improves the solubility and dispersibility of nutrients, making nutrients easier to be absorbed and utilized by plant roots, but also reduces the interaction between nutrients and other components in the soil, reduces the risk of nutrients being fixed, and the nutrients released by the double polymer chelate in the soil can be continuously absorbed by plants, thereby improving soil fertility, and helping to improve soil structure, increase soil permeability, water retention and fertilizer retention; the carboxylic acid derivative in the compound fertilizer used is an organic acid, which can promote the growth and development of plant roots, increase the length and diameter of the roots, and improve the absorption efficiency of plants for nutrient elements. At the same time, the carboxylic acid derivative can also regulate the photosynthesis of plant leaves, enhance the light energy conversion efficiency of plants, enable plants to effectively use sunlight for photosynthesis, generate more energy, and increase crop yields. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Example 1: This example discloses a method for preparing a dimeric chelate, comprising the following steps:

[0034] Q1: 15 g of rice straw was pretreated by heating at 80°C for 12 h in 125 mL of 10 vt% sodium hydroxide solution, then washed with deionized water, dried at 80°C for 12 h, ground to a particle size of 400 mesh, and 2 g of powder was added to 150 mL of 30 vt% hydrogen peroxide solution at 85°C for 8 h, sheared at 4000 rpm for 5 h, washed, filtered, and dispersed in deionized water to obtain a suspension with a concentration of 0.1 g / mL;

[0035] Q2: 6.25 g polyacrylamide, 0.05 g potassium persulfate and 0.0035 g N,N-methylenebispropylene were dissolved in 16 mL deionized water, stirred vigorously for 2 h until completely dissolved, 10 mL of the suspension was added, stirred evenly, 22.5 μL tetramethylethylenediamine was added, and the mixture was heated at 60 °C for 45 min to obtain a gel carrier;

[0036] Q3: 0.025 g of β-glucosidase and 0.015 g of protease were added to 3.5 mL of thiamine pyrophosphate solution, mixed for 60 min, and then 5 g of gel carrier was added, solidified, and dried to obtain a dimeric chelate.

[0037] This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0038] S1: 2.01 g of methyl 2,4-dioxo-4-thiophene-2-ylbutyrate was added to 22.5 mL of methanol, and after stirring, 0.95 g of 2-hydrazinepyridine and 10 mL of hydrochloric acid were added, and the mixture was heated to reflux at 80° C. for 6 h, cooled, and spin-dried, and added to chloroform for dissolution, washed with deionized water, neutralized with sodium bicarbonate solution, extracted, dried with sodium sulfite, and spin-dried to obtain intermediate 1;

[0039] S2: Add 2.76 g of intermediate 1 to a container containing 27.5 mL of anhydrous ethanol, add 6.25 mL of hydrazine hydrate, stir magnetically for 10 h, filter with suction, wash with cold ethanol, and dry to obtain intermediate 2. Dissolve 0.063 g of intermediate 2 and 0.033 g of o-carboxybenzaldehyde in 6.5 mL of anhydrous ethanol, stir at room temperature for 10 h, filter with suction, wash with cold ethanol, and dry to obtain a carboxylic acid derivative;

[0040] S3: 90 g of a carboxylic acid derivative, 1250 g of urea, 1150 g of superphosphate and 1100 g of wood ash were mixed for 3 hours to obtain a compound fertilizer.

[0041] The present invention provides a dimeric biomass method for improving nutrient utilization, comprising the following steps:

[0042] Step 1: Test the soil for nutrients and then plant corn;

[0043] Step 2: Apply the double poly chelate and compound fertilizer by strip application during the seedling stage, jointing stage and fruiting stage of corn;

[0044] Step 3: Harvest the corn according to its growth conditions.

[0045] Example 2: This example discloses a method for preparing a dimeric chelate, comprising the following steps:

[0046] Q1: 10 g of rice straw was pretreated by heating at 80°C for 12 h in 150 mL of 10 vt% sodium hydroxide solution, then washed with deionized water, dried at 80°C for 12 h, ground to a particle size of 400 mesh, and 1 g of powder was added to 200 mL of 30 vt% hydrogen peroxide solution at 85°C for 8 h, sheared at 4000 rpm for 5 h, washed, filtered, and dispersed in deionized water to obtain a suspension with a concentration of 0.1 g / mL;

[0047] Q2: Dissolve 7.5g polyacrylamide, 0.06g potassium persulfate and 0.0045g N,N-methylenebispropylene in 20mL deionized water, stir vigorously for 2h until completely dissolved, add 12mL suspension, stir evenly, add 25μL tetramethylethylenediamine, heat at 60℃ for 45min to obtain a gel carrier;

[0048] Q3: 0.03 g of β-glucosidase and 0.02 g of protease were added to 2 mL of thiamine pyrophosphate solution, mixed for 60 min, and then 4 g of gel carrier was added, solidified, and dried to obtain a dimeric chelate.

[0049] This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0050] S1: 1.78 g of methyl 2,4-dioxo-4-thiophene-2-ylbutyrate was added to 25 mL of methanol, and after stirring, 0.91 g of 2-hydrazinepyridine and 8 mL of hydrochloric acid were added, and the mixture was heated to reflux at 80° C. for 6 h, cooled, and spin-dried, and dissolved in chloroform, washed with deionized water, neutralized with sodium bicarbonate solution, extracted, dried with sodium sulfite, and spin-dried to obtain intermediate 1;

[0051] S2: Add 2.15 g of intermediate 1 to a container containing 30 mL of anhydrous ethanol, add 7.5 mL of hydrazine hydrate, stir magnetically for 10 h, filter with suction, wash with cold ethanol, and dry to obtain intermediate 2. Dissolve 0.057 g of intermediate 2 and 0.031 g of o-carboxybenzaldehyde in 5 mL of anhydrous ethanol, stir at room temperature for 10 h, filter with suction, wash with cold ethanol, and dry to obtain a carboxylic acid derivative;

[0052] S3: 80 g of carboxylic acid derivative, 1000 g of urea, 1400 g of superphosphate and 1000 g of wood ash are mixed for 3 hours to obtain a compound fertilizer.

[0053] The present invention provides a dimeric biomass method for improving nutrient utilization, comprising the following steps:

[0054] Step 1: Test the soil for nutrients and then plant corn;

[0055] Step 2: Apply the double poly chelate and compound fertilizer by strip application during the seedling stage, jointing stage and fruiting stage of corn;

[0056] Step 3: Harvest the corn according to its growth conditions.

[0057] Example 3: This example discloses a method for preparing a dimeric chelate, comprising the following steps:

[0058] Q1: 20 g of rice straw was pretreated by heating at 80°C for 12 h in 120 mL of 10 vt% sodium hydroxide solution, then washed with deionized water, dried at 80°C for 12 h, ground to a particle size of 400 mesh, and 3 g of powder was added to 100 mL of 30 vt% hydrogen peroxide solution at 85°C for 8 h, sheared at 4000 rpm for 5 h, washed, filtered, and dispersed in deionized water to obtain a suspension with a concentration of 0.1 g / mL;

[0059] Q2: 6 g polyacrylamide, 0.04 g potassium persulfate and 0.003 g N,N-methylenebispropylene were dissolved in 12 mL deionized water, stirred vigorously for 2 h until completely dissolved, 8 mL of the suspension was added, stirred evenly, 20 μL tetramethylethylenediamine was added, and the mixture was heated at 60 °C for 45 min to obtain a gel carrier;

[0060] Q3: Add 0.02 g of β-glucosidase and 0.01 g of protease to 5 mL of thiamine pyrophosphate solution, mix for 60 min, add 6 g of gel carrier, solidify, and dry to obtain a dimeric chelate.

[0061] This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0062] S1: 2.21 g of methyl 2,4-dioxo-4-thiophene-2-ylbutyrate was added to 20 mL of methanol, and after stirring, 1.02 g of 2-hydrazinepyridine and 12 mL of hydrochloric acid were added, and the mixture was heated to reflux at 80° C. for 6 h, cooled, and spin-dried, and added to chloroform for dissolution, washed with deionized water, neutralized with sodium bicarbonate solution, extracted, dried with sodium sulfite, and spin-dried to obtain intermediate 1;

[0063] S2: 3.42 g of intermediate 1 was added to a container containing 25 mL of anhydrous ethanol, and 5 mL of hydrazine hydrate was added. The mixture was magnetically stirred for 10 h, filtered, washed with cold ethanol, and dried to obtain intermediate 2. 0.074 g of intermediate 2 and 0.036 g of o-carboxybenzaldehyde were dissolved in 8 mL of anhydrous ethanol, stirred at room temperature for 10 h, filtered, washed with cold ethanol, and dried to obtain a carboxylic acid derivative.

[0064] S3: 100 g of carboxylic acid derivative, 1500 g of urea, 900 g of superphosphate and 1200 g of wood ash were mixed for 3 hours to obtain a compound fertilizer.

[0065] The present invention provides a dimeric biomass method for improving nutrient utilization, comprising the following steps:

[0066] Step 1: Test the soil for nutrients and then plant corn;

[0067] Step 2: Apply the double poly chelate and compound fertilizer by strip application during the seedling stage, jointing stage and fruiting stage of corn;

[0068] Step 3: Harvest the corn according to its growth conditions.

[0069] Example 4: This example discloses a method for preparing a dimeric chelate, comprising the following steps:

[0070] Q1: 12 g of rice straw was pretreated by heating at 80°C for 12 h in 110 mL of 10 vt% sodium hydroxide solution, then washed with deionized water, dried at 80°C for 12 h, ground to a particle size of 400 mesh, and 1.5 g of powder was added to 110 mL of 30 vt% hydrogen peroxide solution at 85°C for 8 h, sheared at 4000 rpm for 5 h, washed, filtered, and dispersed in deionized water to obtain a suspension with a concentration of 0.1 g / mL;

[0071] Q2: 6.5 g polyacrylamide, 0.045 g potassium persulfate and 0.0032 g N,N-methylenebispropylene were dissolved in 14 mL deionized water, stirred vigorously for 2 h until completely dissolved, 9 mL of the suspension was added, stirred evenly, 21 μL tetramethylethylenediamine was added, and the mixture was heated at 60 °C for 45 min to obtain a gel carrier;

[0072] Q3: 0.022 g of β-glucosidase and 0.017 g of protease were added to 3 mL of thiamine pyrophosphate solution, mixed for 60 min, and then 4.5 g of gel carrier was added, solidified, and dried to obtain a dimeric chelate.

[0073] This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0074] S1: 1.87 g of methyl 2,4-dioxo-4-thiophene-2-ylbutyrate was added to 21 mL of methanol, and after stirring, 0.93 g of 2-hydrazinepyridine and 9 mL of hydrochloric acid were added, and the mixture was heated to reflux at 80° C. for 6 h, cooled, and spin-dried, and dissolved in chloroform, washed with deionized water, neutralized with sodium bicarbonate solution, extracted, dried with sodium sulfite, and spin-dried to obtain intermediate 1;

[0075] S2: 2.47 g of intermediate 1 was added to a container containing 26 mL of anhydrous ethanol, and 5.5 mL of hydrazine hydrate was added. The mixture was magnetically stirred for 10 h, filtered, washed with cold ethanol, and dried to obtain intermediate 2. 0.061 g of intermediate 2 and 0.032 g of o-carboxybenzaldehyde were dissolved in 6 mL of anhydrous ethanol, stirred at room temperature for 10 h, filtered, washed with cold ethanol, and dried to obtain a carboxylic acid derivative.

[0076] S3: 85 g of carboxylic acid derivative, 1100 g of urea, 1200 g of superphosphate and 1050 g of wood ash were mixed for 3 hours to obtain a compound fertilizer.

[0077] The present invention provides a dimeric biomass method for improving nutrient utilization, comprising the following steps:

[0078] Step 1: Test the soil for nutrients and then plant corn;

[0079] Step 2: Apply the double poly chelate and compound fertilizer by strip application during the seedling stage, jointing stage and fruiting stage of corn;

[0080] Step 3: Harvest the corn according to its growth conditions.

[0081] Example 5: This example discloses a method for preparing a dimeric chelate, comprising the following steps:

[0082] Q1: 18 g of rice straw was pretreated by heating at 80°C for 12 h in 140 mL of 10 vt% sodium hydroxide solution, then washed with deionized water, dried at 80°C for 12 h, ground to a particle size of 400 mesh, and 2.5 g of powder was added to 170 mL of 30 vt% hydrogen peroxide solution at 85°C for 8 h, sheared at 4000 rpm for 5 h, washed, filtered, and dispersed in deionized water to obtain a suspension with a concentration of 0.1 g / mL;

[0083] Q2: Dissolve 7g polyacrylamide, 0.055g potassium persulfate and 0.0041g N,N-methylenebispropylene in 18mL deionized water, stir vigorously for 2h until completely dissolved, add 11mL suspension, stir evenly, add 24μL tetramethylethylenediamine, heat at 60℃ for 45min to obtain a gel carrier;

[0084] Q3: 0.028 g of β-glucosidase and 0.012 g of protease were added to 4 mL of thiamine pyrophosphate solution, mixed for 60 min, and then 5.5 g of gel carrier was added, solidified, and dried to obtain a dimeric chelate.

[0085] This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0086] S1: 2.03 g of methyl 2,4-dioxo-4-thiophene-2-ylbutyrate was added to 24 mL of methanol, and after stirring, 1.01 g of 2-hydrazinepyridine and 11 mL of hydrochloric acid were added, and the mixture was heated to reflux at 80° C. for 6 h, cooled, and spin-dried, and added to chloroform for dissolution, washed with deionized water, neutralized with sodium bicarbonate solution, extracted, dried with sodium sulfite, and spin-dried to obtain intermediate 1;

[0087] S2: 3.05 g of intermediate 1 was added to a container containing 29 mL of anhydrous ethanol, and 6.5 mL of hydrazine hydrate was added. The mixture was magnetically stirred for 10 h, filtered, washed with cold ethanol, and dried to obtain intermediate 2. 0.072 g of intermediate 2 and 0.034 g of o-carboxybenzaldehyde were dissolved in 7 mL of anhydrous ethanol, stirred at room temperature for 10 h, filtered, washed with cold ethanol, and dried to obtain a carboxylic acid derivative.

[0088] S3: 95 g of carboxylic acid derivative, 1400 g of urea, 1300 g of superphosphate and 1150 g of wood ash were mixed for 3 hours to obtain a compound fertilizer.

[0089] The present invention provides a dimeric biomass method for improving nutrient utilization, comprising the following steps:

[0090] Step 1: Test the soil for nutrients and then plant corn;

[0091] Step 2: Apply the double poly chelate and compound fertilizer by strip application during the seedling stage, jointing stage and fruiting stage of corn;

[0092] Step 3: Harvest the corn according to its growth conditions.

[0093] Example 6: This example discloses a method for preparing a dimeric chelate, comprising the following steps:

[0094] Q1: 16 g of rice straw was pretreated by heating at 80°C for 12 h in 135 mL of 10 vt% sodium hydroxide solution, then washed with deionized water, dried at 80°C for 12 h, ground to a particle size of 400 mesh, and 1.2 g of powder was added to 180 mL of 30 vt% hydrogen peroxide solution at 85°C for 8 h, sheared at 4000 rpm for 5 h, washed, filtered, and dispersed in deionized water to obtain a suspension with a concentration of 0.1 g / mL;

[0095] Q2: Dissolve 7.25g polyacrylamide, 0.043g potassium persulfate and 0.0042g N,N-methylenebispropylene in 15mL deionized water, stir vigorously for 2h until completely dissolved, add 10.5mL suspension, stir evenly, add 22μL tetramethylethylenediamine, heat at 60℃ for 45min to obtain a gel carrier;

[0096] Q3: 0.027 g of β-glucosidase and 0.013 g of protease were added to 4.5 mL of thiamine pyrophosphate solution, mixed for 60 min, and then 4.2 g of gel carrier was added, solidified, and dried to obtain a dimeric chelate.

[0097] This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0098] S1: 2.12 g of methyl 2,4-dioxo-4-thiophene-2-ylbutyrate was added to 23.5 mL of methanol, and after stirring, 0.92 g of 2-hydrazinepyridine and 10 mL of hydrochloric acid were added, and the mixture was heated to reflux at 80° C. for 6 h, cooled, and spin-dried, and added to chloroform for dissolution, washed with deionized water, neutralized with sodium bicarbonate solution, extracted, dried with sodium sulfite, and spin-dried to obtain intermediate 1;

[0099] S2: 2.28 g of intermediate 1 was added to a container containing 28 mL of anhydrous ethanol, and 6.75 mL of hydrazine hydrate was added. The mixture was magnetically stirred for 10 h, filtered, washed with cold ethanol, and dried to obtain intermediate 2. 0.068 g of intermediate 2 and 0.035 g of o-carboxybenzaldehyde were dissolved in 7.5 mL of anhydrous ethanol, stirred at room temperature for 10 h, filtered, washed with cold ethanol, and dried to obtain a carboxylic acid derivative.

[0100] S3: 92 g of carboxylic acid derivative, 1200 g of urea, 1100 g of superphosphate and 1020 g of wood ash were mixed for 3 hours to obtain a compound fertilizer.

[0101] The present invention provides a dimeric biomass method for improving nutrient utilization, comprising the following steps:

[0102] Step 1: Test the soil for nutrients and then plant corn;

[0103] Step 2: Apply the double poly chelate and compound fertilizer by strip application during the seedling stage, jointing stage and fruiting stage of corn;

[0104] Step 3: Harvest the corn according to its growth conditions.

[0105] Comparative Example 1: Compared with Example 1, in the preparation process of the dimerized chelate in Comparative Example 1, no gel carrier is added, and other conditions remain unchanged.

[0106] Comparative Example 2: Compared with Example 1, in Comparative Example 2, no carboxylic acid derivatives were added during the preparation of the compound fertilizer, and other conditions remained unchanged.

[0107] Comparative Example 3: Compared with Example 1, in Comparative Example 3, in the dimeric biological method for improving nutrient utilization, no dimeric chelate is applied, and other conditions remain unchanged.

[0108] Comparative Example 4: Compared with Example 1, in Comparative Example 4, in the double-polymer biological method that can improve nutrient utilization efficiency, no compound fertilizer is applied, and other conditions remain unchanged.

[0109] Comparative Example 5: Compared with Example 1, in Comparative Example 5, in the dual-biological method that can improve nutrient utilization, only nitrogen, phosphorus and potassium compound fertilizers are applied, and other conditions remain unchanged.

[0110] Experimental example: The plant height of corn was measured and the total yield of corn was determined. The results are shown in Table 1:

[0111]

[0112] As can be seen from the test results in Table 1, the methods of Examples 1-6 of the present invention can obtain high corn yields and increase the plant height of corn. Comparison between Comparative Example 1 and Examples 1-6 shows that adding a gel carrier can effectively increase the yield and plant height of corn; comparison between Comparative Example 2 and Examples 1-6 shows that adding a carboxylic acid derivative can effectively increase the yield and plant height of corn; comparison between Comparative Example 3 and Examples 1-6 shows that applying a double polymer chelate can effectively increase the yield and plant height of corn; comparison between Comparative Example 4 and Examples 1-6 shows that applying a compound fertilizer can effectively increase the yield and plant height of corn; comparison between Comparative Example 5 and Examples 1-6 shows that the combined application of a double polymer chelate and a compound fertilizer can effectively increase the yield and plant height of corn.

[0113] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

[0114] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A method for improving nutrient utilization by dipolymerization, characterized in that: The following steps are involved: Step 1: Test the soil for nutrients and then plant the crops; Step 2: Apply double poly chelate and compound fertilizers during the seedling stage, jointing stage and fruiting stage of crops; Step 3: Harvest the crops according to their growth conditions; The preparation method of the dimeric chelate comprises the following steps: Q1: After pre-treatment by heating rice straw in sodium hydroxide solution, washing, drying and grinding, the powder is added to hydrogen peroxide solution under heating conditions, sheared, washed, filtered and dispersed to obtain a suspension. The amount ratio of rice straw to sodium hydroxide solution is (10-20) g: (100-150) mL, the volume fraction of sodium hydroxide solution is 10vt%, and the amount ratio of powder to hydrogen peroxide solution is (1-3) g: (100-200) mL; Q2: Dissolve polyacrylamide, potassium persulfate and N,N-methylenebispropylene in deionized water, stir vigorously until completely dissolved, add the suspension, stir evenly, add tetramethylethylenediamine, heat and polymerize to obtain a gel carrier. The amount ratio of polyacrylamide, potassium persulfate, N,N-methylenebispropylene, deionized water, suspension and tetramethylethylenediamine is (5-7.5) g: (0.04-0.06) g: (0.003-0.0045) g: (12-20) mL: (8-12) mL: (20-25) μL; Q3: Add β-glucosidase and protease to the thiamine pyrophosphate solution, mix well, add a gel carrier, solidify, and dry to obtain a dipolymer chelate. The dosage ratio of thiamine pyrophosphate solution, β-glucosidase, protease, and gel carrier is (2-5) mL: (0.02-0.03) g: (0.01-0.02) g: (4-6) g; The preparation method of the compound fertilizer comprises the following steps: S1: Add methyl 2,4-dioxo-4-thiophene-2-ylbutyrate to methanol, add 2-hydrazinepyridine and hydrochloric acid after stirring, heat to reflux, cool, spin dry, dissolve, wash, neutralize, extract, dry, and spin dry to obtain the intermediate 1, the amount ratio of methyl 2,4-dioxo-4-thiophene-2-ylbutyrate, methanol, 2-hydrazinepyridine and hydrochloric acid is (1.78-2.21) g: (20-25) mL: (0.91-1.02) g: (8-12) mL; S2: Add intermediate 1 to a container containing anhydrous ethanol, add hydrazine hydrate, stir magnetically, filter, wash, and dry to obtain intermediate 2. Dissolve intermediate 2 and o-carboxybenzaldehyde in anhydrous ethanol, stir at room temperature, filter, wash, and dry to obtain a carboxylic acid derivative. The amount ratio of intermediate 1, anhydrous ethanol and hydrazine hydrate is (2.15-3.42) g: (25-30) mL: (5-7.5) mL, and the amount ratio of intermediate 2, o-carboxybenzaldehyde and anhydrous ethanol is (0.057-0.074) g: (0.031-0.036) g: (5-8) mL; S3: Carboxylic acid derivatives, urea, superphosphate and wood ash are mixed to obtain a compound fertilizer, wherein the usage ratio of the carboxylic acid derivatives, urea, superphosphate and wood ash is (80-100) g: (1000-1500) g: (900-1400) g: (1000-1200) g.

2. The method for improving nutrient utilization efficiency according to claim 1, characterized in that: In step 1, the crops are one or more combinations of wheat, corn, soybeans and cotton.

3. The method for improving nutrient utilization efficiency according to claim 1, characterized in that: In the Q1, the heating pretreatment temperature is 80-90°C, the treatment time is 8-12h, the material is washed with deionized water, the drying temperature is 60-80°C, the time is 10-12h, the material is ground to a particle size of 200-400 mesh, the temperature under heating conditions is 80-85°C, the volume fraction of the hydrogen peroxide solution is 30vt%, the treatment time is 6-12h, the shear speed is 3000-4000rpm, the time is 3-5h, and the material is dispersed in deionized water, and the concentration of the suspension is 0.1g / mL.

4. The method for improving nutrient utilization efficiency according to claim 1, characterized in that: In the Q2, the vigorous stirring time is 1-2 hours, the heating polymerization temperature is 60-80° C., and the time is 30-45 minutes.

5. The method for improving nutrient utilization efficiency according to claim 1, characterized in that: In the Q3, the uniform mixing time is 40-60 minutes.

6. The method for improving nutrient utilization efficiency according to claim 1, characterized in that: In the S1, the heating reflux temperature is 80-90°C, the reflux time is 5-7h, the product is added into chloroform for dissolution, washed with deionized water, neutralized with sodium bicarbonate solution, and dried with sodium sulfite.

7. The method for improving nutrient utilization efficiency according to claim 1, characterized in that: In S2, the magnetic stirring time is 8-10 hours, and washing is performed with cold ethanol; the room temperature stirring time is 6-10 hours, and washing is performed with cold ethanol; in S3, the mixing time is 2-4 hours.

8. The method for improving nutrient utilization efficiency according to claim 1, characterized in that: In the step 2, the application method is strip application or broadcast application.

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