A mildew-proof, antibacterial and storage-resistant fish and shrimp feed and its production process

Abstract

The invention discloses a mildew-proof, antibacterial and storage-resistant fish and shrimp feed and a production process thereof, and belongs to the technical field of fish and shrimp feed production. The mildew-proof, antibacterial and storage-resistant fish and shrimp feed provided by the invention comprises the following raw materials in parts by weight: 27 to 35 parts of corn flour, 22 to 25 parts of pea flour, 15 to 20 parts of shrimp shell powder, 5 to 7 parts of fish bone powder, 2 to 3 parts of fish oil, 1 to 2 parts of soybean oil, 0.5 to 1.0 parts of bacterial agent, 0.01 to 0.10 parts of complex vitamins, and 8 to 10 parts of modified binders. The invention prepares the modified binder by introducing a terminal carboxyl monomer on the surface of a substrate, attapulgite. The invention improves the mildew-proof and antibacterial properties of the fish and shrimp feed by adding a self-made modified binder, and comprehensively improves the storage resistance of the fish and shrimp feed.

Description

Technical Field

[0001] The invention belongs to the technical field of fish and shrimp feed production, and particularly relates to mildew-proof, antibacterial, and storage-resistant fish and shrimp feed and a production process thereof. Background Art

[0002] In aquaculture, the quality of fish and shrimp feed is directly related to breeding efficiency and economic benefits. However, feed is easily affected by the environment during storage, especially under high temperature and high humidity conditions, and feed is prone to mildew. The main cause of feed mildew is the growth and reproduction of molds, which consume nutrients in the feed and produce toxins, affecting the palatability and nutritional value of the feed. Specifically, under high temperature and high humidity conditions, such as 35°C ambient temperature and 80% relative humidity, the growth rate of mold will be significantly accelerated. If the feed is stored under such conditions for 14 days, the total number of molds can reach 7.7×10 5 CFU / g, exceeding the national feed hygiene standard by 1×10 5 CFU / g requirements.

[0003] Moldy feed not only reduces nutritional value, but also may produce mycotoxins, posing a serious threat to the health of fish and shrimp. Therefore, it is of great significance to develop a mold-proof, antibacterial and storage-resistant fish and shrimp feed. Summary of the invention

[0004] The purpose of the present invention is to provide a mildew-proof, antibacterial and storage-resistant fish and shrimp feed and a production process thereof, so as to improve the mildew-proof and antibacterial properties of the fish and shrimp feed and ultimately improve the storage resistance of the fish and shrimp feed.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A mildew-proof, antibacterial and storage-resistant fish and shrimp feed, comprising the following raw materials in parts by weight:

[0007] 27-35 parts of corn flour, 22-25 parts of pea flour, 15-20 parts of shrimp shell powder, 5-7 parts of fish bone powder, 2-3 parts of fish oil, 1-2 parts of soybean oil, 0.5-1.0 parts of bacterial agent, 0.01-0.10 parts of complex vitamins, 8-10 parts of modified binder;

[0008] The modified binder is prepared by the following steps:

[0009] S1. Add tocopherol and [3-(trimethoxysilyl)propyl]succinic anhydride to the solvent chloroform with stirring, stir at room temperature for 10 to 20 minutes to obtain a mixed solution, then add Amberlyst-15 ion exchange resin to the mixed solution with stirring, heat the system to 50 to 55° C., stir at constant temperature for 5 to 6 hours, and after the reaction is completed, cool to room temperature, filter to remove the Amberlyst-15 ion exchange resin, and then rotary evaporate to remove the dissolved chloroform to obtain a terminal carboxyl monomer. The above reaction process is as follows:

[0010] .

[0011] S2. Add the terminal carboxyl monomer and attapulgite to the solvent N,N-dimethylformamide with stirring, stir at room temperature for 10 to 20 minutes, then add p-toluenesulfonic acid with stirring, and fill with nitrogen for protection. Heat the system to 40 to 50°C, stir and react at a constant temperature for 5 to 6 hours. After the reaction is completed, cool to room temperature, filter and remove the solvent N,N-dimethylformamide to obtain a solid component, and dry the solid component at 40 to 50°C for 12 hours to obtain a modified binder.

[0012] Furthermore, the bacterial agent is prepared by mixing Bacillus subtilis powder, lactic acid bacteria powder and yeast powder in a mass ratio of 2:2:1 to 2.

[0013] Furthermore, the effective live bacteria content in the Bacillus subtilis powder, lactic acid bacteria powder and yeast powder is 3×10 8 cfu / g~3×10 9 cfu / g.

[0014] Furthermore, the complex vitamin is formed by mixing water-soluble vitamins and fat-soluble vitamins in a mass ratio of 2:1.

[0015] Furthermore, the water-soluble vitamins are formed by mixing vitamin B1, vitamin B2 and folic acid in a mass ratio of 1:1:1.

[0016] Furthermore, the fat-soluble vitamins are formed by mixing vitamin A, vitamin D and vitamin E in a mass ratio of 2 to 3:2:1.

[0017] Furthermore, the tocopherol in S1 is α-tocopherol.

[0018] Furthermore, the usage ratio of chloroform, tocopherol, [3-(trimethoxysilyl)propyl]succinic anhydride and Amberlyst-15 ion exchange resin in S1 is 500 mL: 0.1 mol: 0.11-0.12 mol: 20 g.

[0019] Furthermore, the usage ratio of N,N-dimethylformamide, terminal carboxyl monomer, attapulgite and p-toluenesulfonic acid in S2 is 500mL:35-40g:100g:1-2g.

[0020] Furthermore, a production process of mildew-proof, antibacterial and storage-resistant fish and shrimp feed comprises the following steps:

[0021] The above raw materials are weighed according to mass parts, corn flour, pea flour, shrimp shell flour and fish bone flour are first mixed for 20 to 30 minutes, and then ultrafinely ground and passed through a 200-mesh sieve, and then fish oil, soybean oil, bacterial agent and multivitamin are added thereto, and stirred and mixed for 20 to 30 minutes to obtain a mixture, and then a modified binder is stirred and added to the mixture, and stirred and mixed for 20 to 30 minutes, and then placed in a granulator to make particles with a particle size of 1.2 to 2.0 mm, and dried at 30 to 40°C for 2 to 5 hours to obtain mildew-resistant and storage-resistant fish and shrimp feed.

[0022] Beneficial effects of the present invention:

[0023] The invention provides a mildew-proof, antibacterial and storage-resistant fish and shrimp feed, comprising corn flour, pea flour, shrimp shell flour, fish bone flour, fish oil, soybean oil, bacterial agent, compound vitamins and modified binder. The mildew-proof and antibacterial properties of the fish and shrimp feed are improved by adding a self-made modified binder, and the storage resistance of the fish and shrimp feed is comprehensively improved. Specifically, the invention prepares the modified binder by introducing a terminal carboxyl monomer on the surface of a substrate, attapulgite.

[0024] First of all, attapulgite is a natural hydrated magnesium-aluminum silicate mineral, whose surface contains abundant active sites (silicon hydroxyl, aluminum hydroxyl and magnesium hydroxyl) and a unique rod crystal structure. The rod crystal structure of attapulgite can be highly dispersed in water. Under the electrostatic action of the end faces and the positive and negative charges on the surface, a three-dimensional spatial network structure of overlapping rod crystals is formed, showing good thickening and thixotropy. Therefore, the present invention uses it as a base material for the binder of fish and shrimp feed. Then, attapulgite itself has excellent antibacterial properties. Tachygorskite can be combined with bacteria through physical adsorption, affecting the permeability of bacterial cell membranes and preventing normal material exchange between bacteria and the environment. At the same time, the rod crystals of attapulgite have a "needle effect", which can destroy the cell wall and cell membrane of the cell, making the bacteria inactive.

[0025] However, since the surface of attapulgite contains abundant hydroxyl groups, it has strong hydrophilicity, and the fish and shrimp feed prepared by using it as a binder has strong hygroscopicity. The fish and shrimp feed with strong hygroscopicity is more likely to absorb moisture in the environment during storage, causing the fish and shrimp feed to agglomerate and mildew, shortening the shelf life of the fish and shrimp feed. Therefore, the present invention introduces a terminal carboxyl monomer on the surface of the substrate attapulgite, aiming to reduce the water absorption of the finally prepared fish and shrimp feed while improving the mildew and antibacterial properties of the fish and shrimp feed.

[0026] Thus, the present invention uses a self-made terminal carboxyl monomer to modify the surface of attapulgite, and the terminal carboxyl monomer is prepared by esterification reaction of hydroxyl-containing tocopherol and [3-(trimethoxysilyl)propyl] succinic anhydride containing an acid anhydride group, and then the terminal carboxyl group in the self-made terminal carboxyl monomer is used to carry out esterification reaction with the active hydroxyl group on the surface of attapulgite to achieve surface modification of attapulgite. Specifically, the side chain of tocopherol is hydrophobic, and the hydrophobic structure of tocopherol can reduce the hygroscopicity of the modified binder. At the same time, tocopherol has antioxidant properties, which can further improve the antioxidant properties of the finally prepared fish and shrimp feed, thereby reducing the degree of mildew of the fish and shrimp feed. In order to successfully graft tocopherol onto the surface of attapulgite, the present invention utilizes [3-(trimethoxysilyl)propyl]succinic anhydride to carry out an esterification reaction with tocopherol to obtain a terminal carboxyl monomer, and then utilizes the terminal carboxyl to react with the abundant hydroxyl groups on the surface of attapulgite to achieve the purpose of grafting tocopherol onto the surface of attapulgite; further, [3-(trimethoxysilyl)propyl]succinic anhydride contains a siloxane chain, and the introduction of the siloxane chain helps to improve the compatibility between the modified binder and other components in the fish and shrimp feed. At the same time, since the siloxane chain has a lower surface tension, it gives the finally prepared fish and shrimp feed excellent surface activity, helps to reduce the load of bacteria and molds, and helps to improve the mildew and antibacterial properties of the fish and shrimp feed.

[0027] Finally, after testing, it was found that by adding the homemade modified adhesive of the present invention, the mildew resistance and antibacterial properties of the fish and shrimp feed were significantly improved, and the storage stability of the fish and shrimp feed was significantly improved. DETAILED DESCRIPTION

[0028] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0029] Example 1

[0030] Preparation of modified binder:

[0031] S1, stirring tocopherol (α-tocopherol; ≥95.5%; purchased from Sigma-Aldrich Trading Co., Ltd.) and [3-(trimethoxysilyl)propyl]succinic anhydride (95%; purchased from Sinopharm Chemical Reagent Co., Ltd.) were added to the solvent chloroform (AR; purchased from Sinopharm Chemical Reagent Co., Ltd.), and stirred at room temperature for 10 min to obtain a mixed solution, and then Amberlyst-15 ion exchange resin (purchased from Sinopharm Chemical Reagent Co., Ltd.) was added to the mixed solution with stirring, the system was heated to 50° C., and the reaction was stirred at a constant temperature for 5 h. After the reaction was completed, it was cooled to room temperature, the Amberlyst-15 ion exchange resin was filtered out, and the dissolved chloroform was removed by rotary evaporation to obtain a terminal carboxyl monomer, wherein the amount ratio of chloroform, tocopherol, [3-(trimethoxysilyl)propyl]succinic anhydride and Amberlyst-15 ion exchange resin was 500 mL: 0.1 mol: 0.11 mol: 20 g;

[0032] S2. Add carboxyl-terminated monomer and attapulgite (purchased from Tianjin Shenlite Biotechnology Co., Ltd.) into solvent N,N-dimethylformamide (AR; ≥95.5%; purchased from Sinopharm Chemical Reagent Co., Ltd.) with stirring. After stirring at room temperature for 10 min, add p-toluenesulfonic acid (99%; purchased from Shanghai MacLean Biochemical Technology Co., Ltd.) with stirring, and fill with nitrogen for protection. The system is heated to 40°C and stirred at a constant temperature for 5 h. After the reaction is completed, it is cooled to room temperature, and the solvent N,N-dimethylformamide is filtered to remove solid components. The solid components are dried at 40°C for 12 h to obtain a modified binder, wherein the amount ratio of N,N-dimethylformamide, carboxyl-terminated monomer, attapulgite and p-toluenesulfonic acid is 500 mL:35 g:100 g:1 g.

[0033] Example 2

[0034] Preparation of modified binder:

[0035] S1, stirring tocopherol (α-tocopherol; ≥95.5%; purchased from Sigma-Aldrich Trading Co., Ltd.) and [3-(trimethoxysilyl)propyl]succinic anhydride (95%; purchased from Sinopharm Chemical Reagent Co., Ltd.) were added to the solvent chloroform (AR; purchased from Sinopharm Chemical Reagent Co., Ltd.), and stirred at room temperature for 20 minutes to obtain a mixed solution, and then Amberlyst-15 ion exchange resin (purchased from Sinopharm Chemical Reagent Co., Ltd.) was added to the mixed solution with stirring, the system was heated to 55° C., and the reaction was stirred at a constant temperature for 6 hours. After the reaction was completed, it was cooled to room temperature, the Amberlyst-15 ion exchange resin was filtered to remove the Amberlyst-15 ion exchange resin, and the dissolved chloroform was then removed by rotary evaporation to obtain a terminal carboxyl monomer, wherein the amount ratio of chloroform, tocopherol, [3-(trimethoxysilyl)propyl]succinic anhydride and Amberlyst-15 ion exchange resin was 500mL:0.1mol:0.12mol:20g;

[0036] S2. Add carboxyl-terminated monomer and attapulgite (purchased from Tianjin Shenlite Biotechnology Co., Ltd.) into solvent N,N-dimethylformamide (AR; ≥95.5%; purchased from Sinopharm Chemical Reagent Co., Ltd.) with stirring. After stirring at room temperature for 20 min, add p-toluenesulfonic acid (99%; purchased from Shanghai MacLean Biochemical Technology Co., Ltd.) with stirring, and fill with nitrogen for protection. The system is heated to 45°C and stirred at a constant temperature for 6 h. After the reaction is completed, it is cooled to room temperature, and the solvent N,N-dimethylformamide is filtered out to obtain a solid component. The solid component is dried at 45°C for 12 h to obtain a modified binder, wherein the amount ratio of N,N-dimethylformamide, carboxyl-terminated monomer, attapulgite and p-toluenesulfonic acid is 500 mL:38 g:100 g:1.5 g.

[0037] Example 3

[0038] Preparation of modified binder:

[0039] S1, stirring tocopherol (α-tocopherol; ≥95.5%; purchased from Sigma-Aldrich Trading Co., Ltd.) and [3-(trimethoxysilyl)propyl]succinic anhydride (95%; purchased from Sinopharm Chemical Reagent Co., Ltd.) were added to the solvent chloroform (AR; purchased from Sinopharm Chemical Reagent Co., Ltd.), and stirred at room temperature for 20 minutes to obtain a mixed solution, and then Amberlyst-15 ion exchange resin (purchased from Sinopharm Chemical Reagent Co., Ltd.) was added to the mixed solution with stirring, the system was heated to 55° C., and the reaction was stirred at a constant temperature for 6 hours. After the reaction was completed, it was cooled to room temperature, the Amberlyst-15 ion exchange resin was filtered to remove the Amberlyst-15 ion exchange resin, and the dissolved chloroform was then removed by rotary evaporation to obtain a terminal carboxyl monomer, wherein the amount ratio of chloroform, tocopherol, [3-(trimethoxysilyl)propyl]succinic anhydride and Amberlyst-15 ion exchange resin was 500mL:0.1mol:0.12mol:20g;

[0040] S2. Add carboxyl-terminated monomer and attapulgite (purchased from Tianjin Shenlite Biotechnology Co., Ltd.) into solvent N,N-dimethylformamide (AR; ≥95.5%; purchased from Sinopharm Chemical Reagent Co., Ltd.) with stirring. After stirring at room temperature for 20 minutes, add p-toluenesulfonic acid (99%; purchased from Shanghai MacLean Biochemical Technology Co., Ltd.) with stirring, and fill with nitrogen for protection. The system is heated to 50°C and stirred at a constant temperature for 6 hours. After the reaction is completed, it is cooled to room temperature, and the solvent N,N-dimethylformamide is filtered to remove solid components. The solid components are dried at 50°C for 12 hours to obtain a modified binder, wherein the amount ratio of N,N-dimethylformamide, carboxyl-terminated monomer, attapulgite and p-toluenesulfonic acid is 500mL:40g:100g:2g.

[0041] Comparative Example 1

[0042] Comparative Example 1 is the control group of Example 2, in which [3-(trimethoxysilyl)propyl]succinic anhydride in Example 2 is replaced by succinic anhydride, and the remaining raw materials, raw material amounts and production process remain unchanged, and finally a modified binder is obtained, and the specific preparation process is as follows:

[0043] S1. Add tocopherol (α-tocopherol; ≥95.5%; purchased from Sigma-Aldrich Trading Co., Ltd.) and succinic anhydride (purchased from Sinopharm Chemical Reagent Co., Ltd.) to the solvent chloroform (AR; purchased from Sinopharm Chemical Reagent Co., Ltd.) with stirring, and stir at room temperature for 20 minutes to obtain a mixed solution, and then add Amberlyst-15 ion exchange resin (purchased from Sinopharm Chemical Reagent Co., Ltd.) to the mixed solution with stirring, and heat the system to 55° C. and stir at a constant temperature for 6 hours. After the reaction is completed, cool to room temperature, filter to remove the Amberlyst-15 ion exchange resin, and then rotary evaporate to remove the dissolved chloroform to obtain a terminal carboxyl monomer, wherein the amount ratio of chloroform, tocopherol, succinic anhydride and Amberlyst-15 ion exchange resin is 500 mL: 0.1 mol: 0.12 mol: 20 g;

[0044] S2. Add carboxyl-terminated monomer and attapulgite (purchased from Tianjin Shenlite Biotechnology Co., Ltd.) into solvent N,N-dimethylformamide (AR; ≥95.5%; purchased from Sinopharm Chemical Reagent Co., Ltd.) with stirring. After stirring at room temperature for 20 min, add p-toluenesulfonic acid (99%; purchased from Shanghai MacLean Biochemical Technology Co., Ltd.) with stirring, and fill with nitrogen for protection. The system is heated to 45°C and stirred at a constant temperature for 6 h. After the reaction is completed, it is cooled to room temperature, and the solvent N,N-dimethylformamide is filtered out to obtain a solid component. The solid component is dried at 45°C for 12 h to obtain a modified binder, wherein the amount ratio of N,N-dimethylformamide, carboxyl-terminated monomer, attapulgite and p-toluenesulfonic acid is 500 mL:38 g:100 g:1.5 g.

[0045] Example 4

[0046] A production process of mildew-proof, antibacterial and storage-resistant fish and shrimp feed:

[0047] Firstly, the mildew-proof, antibacterial and storage-resistant fish and shrimp feed includes the following raw materials by weight:

[0048] 27 parts of corn flour, 22 parts of pea flour, 15 parts of shrimp shell powder, 5 parts of fish bone powder, 2 parts of fish oil, 1 part of soybean oil, 0.5 parts of bacterial agent, 0.01 parts of multivitamins, and 8 parts of the modified binder prepared in Example 1;

[0049] Then, the production process of mildew-proof, antibacterial and storage-resistant fish and shrimp feed includes the following steps:

[0050] The above raw materials were weighed according to their mass proportions. First, corn flour, pea flour, shrimp shell flour and fish bone flour were mixed for 20 minutes, and then ultrafinely ground and passed through a 200-mesh sieve. Fish oil, soybean oil, bacterial agent and multivitamin were added thereto, and the mixture was stirred and mixed for 20 minutes to obtain a mixture. The modified binder prepared in Example 1 was stirred and mixed for 20 minutes, and then placed in a granulator to prepare granules with a particle size of 2.0 mm. The granules were dried at 30°C for 2 hours to obtain mildew-resistant and storage-resistant fish and shrimp feed.

[0051] Example 5

[0052] A production process of mildew-proof, antibacterial and storage-resistant fish and shrimp feed:

[0053] Firstly, the mildew-proof, antibacterial and storage-resistant fish and shrimp feed includes the following raw materials by weight:

[0054] 30 parts of corn flour, 24 parts of pea flour, 18 parts of shrimp shell powder, 6 parts of fish bone powder, 3 parts of fish oil, 2 parts of soybean oil, 0.7 parts of bacterial agent, 0.05 parts of multivitamins, and 10 parts of the modified binder prepared in Example 2;

[0055] Then, the production process of mildew-proof, antibacterial and storage-resistant fish and shrimp feed includes the following steps:

[0056] The above raw materials were weighed according to their mass proportions. First, corn flour, pea flour, shrimp shell flour and fish bone flour were mixed for 30 minutes, and then ultrafinely ground and passed through a 200-mesh sieve. Fish oil, soybean oil, bacterial agent and multivitamin were added thereto, and the mixture was stirred and mixed for 30 minutes to obtain a mixture. The modified binder prepared in Example 2 was stirred and mixed for 30 minutes, and then placed in a granulator to prepare granules with a particle size of 1.2 mm. The granules were dried at 40°C for 5 hours to obtain mildew-resistant and storage-resistant fish and shrimp feed.

[0057] Example 6

[0058] A production process of mildew-proof, antibacterial and storage-resistant fish and shrimp feed:

[0059] Firstly, the mildew-proof, antibacterial and storage-resistant fish and shrimp feed includes the following raw materials by weight:

[0060] 35 parts of corn flour, 25 parts of pea flour, 20 parts of shrimp shell powder, 7 parts of fish bone powder, 3 parts of fish oil, 2 parts of soybean oil, 1.0 part of bacterial agent, 0.10 part of multivitamins, and 10 parts of the modified binder prepared in Example 3;

[0061] Then, the production process of mildew-proof, antibacterial and storage-resistant fish and shrimp feed includes the following steps:

[0062] The above raw materials were weighed according to their mass proportions. First, corn flour, pea flour, shrimp shell flour and fish bone flour were mixed for 30 minutes, and then ultrafinely ground and passed through a 200-mesh sieve. Fish oil, soybean oil, bacterial agent and multivitamin were added thereto, and the mixture was stirred for 30 minutes to obtain a mixture. The modified binder prepared in Example 3 was stirred and mixed for 30 minutes, and then placed in a granulator to prepare granules with a particle size of 1.5 mm. The granules were dried at 40°C for 5 hours to obtain mildew-resistant and storage-resistant fish and shrimp feed.

[0063] Comparative Example 2

[0064] Comparative Example 2 is the control group of Example 5, in which the modified binder prepared in Example 2 in Example 5 is replaced by the modified binder prepared in Comparative Example 1, and the other raw materials, raw material amounts and production steps remain unchanged, and fish and shrimp feed is finally obtained.

[0065] Comparative Example 3

[0066] Comparative Example 3 is the control group of Example 5, in which the modified binder prepared in Example 2 in Example 5 is replaced with attapulgite (purchased from Tianjin Shenlite Biotechnology Co., Ltd.), and the other raw materials, raw material amounts and production steps remain unchanged, and fish and shrimp feed is finally obtained.

[0067] The performance test of the fish and shrimp feed prepared in Examples 4 to 6 and Comparative Examples 2 to 3 was performed. The performance test process is as follows, and the test results are shown in Table 1:

[0068] Water absorption rate: 10 g of fish and shrimp feed prepared in Examples 4 to 6 and Comparative Examples 2 to 3 were respectively placed in 100 mL of deionized water, and the feed was covered with a plastic block to ensure that it was completely immersed in deionized water, and allowed to stand for 5 minutes, filtered, and the solid components were collected, and then placed in a 50°C drying oven for 10 minutes to make the feed reach a semi-dry state, and then the weight in the semi-dry state was weighed, recorded as m, and the water absorption rate was calculated according to the following formula, and recorded in the following Table 1:

[0069] Water absorption rate = [(m-10) / 10]×100%.

[0070] Anti-mildew performance: The fish and shrimp feeds prepared in Examples 4 to 6 and Comparative Examples 2 to 3 were placed in a constant temperature, constant humidity, breathable and light-proof environment at 35°C and 80% humidity for 10 days, and the mold content was determined by referring to the method in GB / T13092-2006 "Determination of the total number of molds in feeds". The feeds of the same group of Examples / Comparative Examples were measured five times and the average value was calculated and recorded in the following Table 1:

[0071] Antibacterial performance: 0.1 g of fish and shrimp feed prepared in Examples 4 to 6 and Comparative Examples 2 to 3 were mixed with 10 mL of 1×10 7The mixture of Escherichia coli liquid and Staphylococcus aureus liquid was placed in a test tube and placed on a constant temperature shaker at 37°C and 120 r / min for 4 h. This was used as the experimental group, and the unmixed feed was used as the blank group. The bacterial liquid of the experimental group and the blank group was taken out and diluted to 1×10 5 CFU / mL, 100 μL of bacterial solution was applied to the surface of the agar plate, and incubated in an oscillating incubator at room temperature for 24 h. The antibacterial rate was calculated by the plate colony counting method and recorded in the following Table 1:

[0072] Antibacterial rate (%) = [(N a -N b ) / Na]×100%;

[0073] Among them, N a is the colony count of the blank group; N b is the colony number of the experimental group.

[0074] Table 1 Test results

[0075] ;

[0076] It can be seen from Table 1 that the fish and shrimp feeds prepared in Examples 4 to 6 of the present invention have excellent mildew resistance, antibacterial and storage resistance properties.

[0077] It should be noted that, in this article, terms such as "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or equipment that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or equipment.

[0078] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A mildew-proof, antibacterial and storage-resistant fish and shrimp feed, characterized in that: Including the following raw materials by weight: 27-35 parts of corn flour, 22-25 parts of pea flour, 15-20 parts of shrimp shell powder, 5-7 parts of fish bone powder, 2-3 parts of fish oil, 1-2 parts of soybean oil, 0.5-1.0 parts of bacterial agent, 0.01-0.10 parts of complex vitamins, 8-10 parts of modified binder; The modified binder is prepared by the following steps: S1, adding tocopherol and [3-(trimethoxysilyl)propyl]succinic anhydride to the solvent chloroform with stirring, stirring at room temperature for 10 to 20 minutes to obtain a mixed solution, and then adding Amberlyst-15 ion exchange resin to the mixed solution with stirring, heating the system to 50 to 55° C., stirring at a constant temperature for 5 to 6 hours, cooling to room temperature after the reaction is completed, filtering to remove the Amberlyst-15 ion exchange resin, and then rotary evaporating to remove the dissolved chloroform to obtain a terminal carboxyl monomer; S2, adding the carboxyl-terminated monomer and attapulgite to the solvent N,N-dimethylformamide with stirring, stirring at room temperature for 10 to 20 minutes, and then adding p-toluenesulfonic acid with stirring, and charging with nitrogen for protection, heating the system to 40 to 50° C., stirring and reacting at a constant temperature for 5 to 6 hours, cooling to room temperature after the reaction is completed, filtering to remove the solvent N,N-dimethylformamide, and obtaining a solid component, and drying the solid component at 40 to 50° C. for 12 hours to obtain a modified binder; The tocopherol described in S1 is α-tocopherol.

2. The mildew-proof, antibacterial and storage-resistant fish and shrimp feed according to claim 1, characterized in that: The microbial agent is prepared by mixing Bacillus subtilis powder, lactic acid bacteria powder and yeast powder in a mass ratio of 2:2:1-2.

3. The mildew-proof, antibacterial and storage-resistant fish and shrimp feed according to claim 2, characterized in that: The effective live bacteria content in Bacillus subtilis powder, lactic acid bacteria powder and yeast powder is 3×10 8 cfu / g~3×10 9 cfu / g.

4. The mildew-proof, antibacterial and storage-resistant fish and shrimp feed according to claim 1, characterized in that: Multivitamins are a mixture of water-soluble vitamins and fat-soluble vitamins in a mass ratio of 2:

1.

5. The mildew-proof, antibacterial and storage-resistant fish and shrimp feed according to claim 4, characterized in that: The water-soluble vitamins are mixed with vitamin B1, vitamin B2 and folic acid in a mass ratio of 1:1:

1.

6. The mildew-proof, antibacterial and storage-resistant fish and shrimp feed according to claim 4, characterized in that: The fat-soluble vitamins are formed by mixing vitamin A, vitamin D and vitamin E in a mass ratio of 2 to 3:2:

1.

7. The mildew-proof, antibacterial and storage-resistant fish and shrimp feed according to claim 1, characterized in that: The usage ratio of chloroform, tocopherol, [3-(trimethoxysilyl)propyl]succinic anhydride and Amberlyst-15 ion exchange resin in S1 is 500 mL: 0.1 mol: 0.11-0.12 mol: 20 g.

8. The mildew-proof, antibacterial and storage-resistant fish and shrimp feed according to claim 1, characterized in that: The usage ratio of N,N-dimethylformamide, terminal carboxyl monomer, attapulgite and p-toluenesulfonic acid in S2 is 500mL:35-40g:100g:1-2g.

9. A production process for mildew-proof, antibacterial and storage-resistant fish and shrimp feed according to any one of claims 1 to 8, characterized in that: The following steps are involved: The above raw materials are weighed according to mass parts. First, corn flour, pea flour, shrimp shell flour and fish bone flour are mixed for 20 to 30 minutes, and then ultrafinely ground and passed through a 200-mesh sieve. Fish oil, soybean oil, bacterial agent and multivitamin are added thereto, and stirred for 20 to 30 minutes to obtain a mixture. The modified binder is stirred into the mixture, and the mixture is stirred for 20 to 30 minutes. The mixture is placed in a granulator to form particles with a particle size of 1.2 to 2.0 mm, and dried at 30 to 40° C. for 2 to 5 hours to obtain mildew-proof, antibacterial and storage-resistant fish and shrimp feed.