Human albumin anti-aging essence and preparation method thereof

Abstract

The invention relates to the field of protein cosmetics, in particular to human albumin anti-aging essence and a preparation method thereof. The human albumin anti-aging essence is prepared from the following components in parts by mass: 20 to 45 parts of human albumin; 5-15 parts of a red ginseng extract; 2.5-10 parts of a vitis amurensis seed extract; 1.5 to 5 parts of a blueberry peel extract; 20 to 40 parts of a humectant; 0.2 to 1.6 parts of a thickening agent; 0.01 to 0.1 part of an excipient; and the balance of deionized water. The human albumin anti-aging essence can be used for skin aging resistance, has the effects of resisting free radicals, increasing skin elasticity and the like, has good biocompatibility and effectiveness, and has important significance on skin aging resistance and skin improvement.

Description

Technical Field

[0001] The invention relates to the field of protein cosmetics, and in particular to a human albumin anti-aging essence and a preparation method thereof. Background Art

[0002] With the continuous development of social economy and the improvement of people's living standards, the demand for cosmetics is constantly increasing. Skin care and anti-aging have become the research focus in the field of cosmetics. Aging is mainly manifested as dry, rough, wrinkled, dull, inelastic, pale and loose skin, and even skin atrophy, wrinkles and age spots. Skin aging not only affects the appearance and image of the human body, but also seriously hinders the physiological function of the human skin and even causes serious pathological changes in the skin. Therefore, the anti-aging effect of cosmetics has become a top priority.

[0003] Traditional anti-aging products are mainly achieved by adding raw materials with the effects of promoting collagen and elastin synthesis, promoting fibroblast growth, anti-oxidation, and preventing ultraviolet radiation. These raw materials are often plant extracts and chemical raw materials, and there are few protein raw materials. CN111568831B provides a composition of Chinese herbal extracts for skin cosmetics, which contains Chinese herbal extracts of chrysanthemum, hawkweed, angelica, Panax notoginseng, astragalus, Ligustrum lucidum, licorice and elderberry. CN106491455B discloses a composition containing a large-leaf buddleja extract, which is mainly made of the following components in parts by weight: 20-70 parts of a large-leaf buddleja extract; 10-30 parts of an algae composite extract. The above compositions all contain only plant extract raw materials, which are derived from plants and have complex ingredients, resulting in a low content of active ingredients and may also produce side effects. Cosmetics using chemical raw materials often need to add preservatives to achieve stable and long-term preservation. In summary, it is difficult for existing cosmetics to simultaneously meet biocompatibility and antioxidant effects, and it is difficult to meet the needs of skin anti-aging. Summary of the invention

[0004] In order to solve the deficiencies of the prior art, the present invention provides a human albumin anti-aging essence and a preparation method thereof. The human albumin anti-aging essence contains recombinant human albumin, red ginseng extract, wild grape seed extract, blueberry peel extract, moisturizer, thickener and excipient, can be used for skin anti-aging, has the effects of anti-free radicals, increasing skin elasticity, etc., has good biocompatibility and effectiveness, and is of great significance for skin anti-aging and skin improvement.

[0005] In order to achieve the above object, the present invention is implemented through the following technical solutions: In a first aspect, the present invention provides a human albumin anti-aging essence, which comprises the following components in parts by mass: Human albumin 20-45 parts; 5-15 parts of red ginseng extract; 2.5-10 parts of Vitis vinifera seed extract; 1.5-5 parts of blueberry peel extract; 20~40 parts of moisturizer; Thickener 0.2~1.6 parts; Excipient 0.01~0.1 part; The balance was deionized water.

[0006] Human serum albumin (HSA) is a monomeric protein produced by the liver. Mature human serum albumin contains 585 amino acid residues and has a molecular weight of 66KD. Albumin has abundant free sulfhydryl groups and antioxidant properties. It can scavenge free radicals (such as hypochlorous acid (HOCl) and peroxynitrite (ONOOH)) and provide sulfhydryl groups (-SH) to produce anti-aging effects.

[0007] The Chinese Pharmacopoeia (2020 edition) records that red ginseng (Ginseng Radix et Rhizoma Rubra, GRRR) is the steamed dried root and rhizome of the cultivated product of Panax ginseng CA Mey. of the Araliaceae family. Red ginseng is warm in nature, sweet and slightly bitter in taste, and has the effects of greatly replenishing vital energy, restoring pulse and strengthening deficiency, and replenishing qi and blood. Red ginseng is a processed product of ginseng. The function of processed red ginseng is biased towards warming and nourishing, and it is mainly used for symptoms of spleen and kidney deficiency and cold, and true yang decline. Studies have shown that because red ginseng contains ginsenosides, ginsenodiols, ginsenotriols and other ingredients, it has the effects of anti-cholesterol, improving skin nutrition, and can play a role in delaying skin aging.

[0008] Grape production is increasing year by year with the increasing demand for grape drinks and fresh products. However, a lot of products other than grapes are wasted, such as grape seeds and grape skins produced after making wine or grape juice, which are treated as waste. Studies have shown that wild grape seeds contain a large amount of anthocyanins and unsaturated fatty acids, which have multiple biological activities, such as anti-inflammatory, antioxidant, bactericidal, anti-cancer and antiviral effects.

[0009] Blueberry is scientifically known as Vaccinium vitis-idaea. It is a perennial deciduous or evergreen fruit tree belonging to the genus Vaccinium of the Ericaceae family. It is a shrub. Its English name is Blueberry. Blueberry skin contains many kinds of amino acids, as well as rich minerals and a large number of bioactive substances, such as anthocyanins and flavonoids. Its bioactive substances have anti-allergic, anti-viral, anti-inflammatory and antioxidant effects.

[0010] Preferably, the human albumin comprises recombinant human albumin.

[0011] Further preferably, the recombinant human albumin is an albumin with high expression and low O-glycosylation level.

[0012] Preferably, the mass fraction of human albumin is 25 to 30 parts.

[0013] Preferably, the red ginseng extract, Vitis vinifera seed extract and blueberry peel extract are obtained by extraction with 75% ethanol, filtration and distillation.

[0014] Preferably, the moisturizer includes at least one of glycerol, ethylhexylglycerol, 1,3-propylene glycol, 1,3-butylene glycol, 1,2-hexanediol and 1,2-pentanediol, the thickener includes at least one of sodium alginate, calcium alginate, potassium alginate, alginic acid and sodium lactate, and the excipient includes at least one of hydroxymethyl cellulose, hydroxyethyl cellulose, methyl cellulose and hydroxypropyl methyl cellulose.

[0015] Preferably, the mass fraction of the red ginseng extract is 10 to 12 parts.

[0016] Preferably, the mass fraction of the Vitis vinifera seed extract is 6 to 8 parts.

[0017] Preferably, the mass fraction of the blueberry peel extract is 2 to 3.5 parts.

[0018] Preferably, the mass fraction of the glycerol is 30-35 parts.

[0019] Preferably, the mass fraction of the sodium alginate is 0.8 to 1.0 parts.

[0020] Preferably, the mass fraction of the carboxymethyl cellulose is 0.05-0.08 parts.

[0021] In a second aspect, the present invention provides a method for preparing the human albumin anti-aging essence as described in the first aspect, comprising the following steps: Red ginseng extract, wild grape seed extract, blueberry peel extract, moisturizer, thickener, excipient and deionized water are stirred to prepare a mixed solution, the mixed solution is added to human albumin and deionized water is added to make up the balance, and the human albumin anti-aging essence is obtained by stirring, dissolving, sterilizing and filtering.

[0022] Preferably, the temperature for stirring the mixed solution is 50° C. to 70° C. for 5 to 15 min, and the mixed solution is added to human albumin after being cooled.

[0023] Preferably, the stirring speed of the stirring dissolution is 50-80 rpm, the time is 20-45 min, and the pore size of the sterilization filtration is 0.22 µm.

[0024] Preferably, the preparation of red ginseng extract, wild grape seed extract and blueberry peel extract is also included: Red ginseng, wild grape seed and blueberry peel were crushed and sieved to obtain red ginseng powder, wild grape seed powder and blueberry peel powder respectively. The red ginseng powder, wild grape seed powder and blueberry peel powder were respectively soaked in 75% ethanol and then refluxed for extraction. The filtrates were combined and the ethanol was distilled off until there was no alcohol taste to obtain red ginseng extract, wild grape seed extract and blueberry peel extract.

[0025] Further preferably, the soaking material-liquid ratio is 1:(5-10), the soaking time is 5-7 h, and the reflux extraction is performed 2-4 times, each reflux extraction is 10-20 min.

[0026] Preferably, the red ginseng extract, wild grape seed extract and blueberry peel extract are resuspended in water according to their mass fractions to prepare solutions, the solutions are mixed and spray-dried to prepare a mixture for use, the inlet air temperature of the spray drying is 120-160°C, the atomization pressure is 0.1-1.0 Mpa, and the feed rate is 200-800 mL / h.

[0027] The beneficial effects achieved by one or more technical solutions of the present invention are as follows: The human albumin anti-aging essence of the present invention uses human albumin as a protein component and plant components consisting of red ginseng extract, wild grape seed extract, and blueberry peel extract as main components. The protein component and the plant component act synergistically. Compared with commercially available cosmetics, the effective ingredients are simplified, the antioxidant activity is improved, and the storage is simple.

[0028] The human albumin anti-aging essence of the present invention can effectively promote the proliferation of fibroblasts and alleviate skin aging problems.

[0029] The human albumin anti-aging essence of the invention has simple preparation process, excellent indicators and application prospects. DETAILED DESCRIPTION

[0030] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in combination with specific embodiments and comparative examples.

[0031] Definition: Recombinant human albumin refers to albumin prepared using recombinant DNA technology for treatment and prevention.

[0032] Source of materials: Recombinant human albumin: Tonghua Anrui Special Biopharmaceutical Co., Ltd., batch number: ART101S; Red ginseng was purchased from Kangmei Xinkaihe (Jilin) ​​Pharmaceutical Co., Ltd., batch number: 20231120; Wild grape seeds were provided by Tonghua Winery; The blueberry peels are provided by the Tonghua blueberry industrial base.

[0033] equipment: Water bath (HH.S21-8, Shanghai Boxun Medical Biological Instrument Co., Ltd.); Multifunctional fluorescence microplate reader (SPMax 3500FL, Shanghai Shanpu Biotechnology Co., Ltd.); Analytical balance (BSA224S, Sartorius Scientific Instruments Beijing Co., Ltd.); DPPH: (Batch No.: D273092-100mg, Shanghai Aladdin Biochemical Technology Co., Ltd.); Vitamin C (ascorbic acid) (Batch No.: A103533-100g, Shanghai Aladdin Biochemical Technology Co., Ltd.); CCK-8 kit (Batch No.: 40203ES60, Yisheng Biotechnology (Shanghai) Co., Ltd.); Fibroblast medium-serum-free FM-sf (Cat. No. 2311, Beijing Yuhengfeng Technology Co., Ltd.); pH meter (PB-10, Sartorius Scientific Instruments Beijing Co., Ltd.); Facial image analysis: VISIA image analysis software (Canfied, USA); Skin elasticity test probe Cutometer (MPA580, CK Company, Germany); Skin microscope and active skin surface analysis system VisioScan (VC20plus, CK Company, Germany).

[0034] Unless otherwise specified, other reagents and instruments used were commercially available.

[0035] Example 1 Preparation of recombinant human albumin - high expression and low O-glycosylation level albumin Take the preserved strain CBS7435-rHSA (disclosed in the prior patent CN118006716B) for plate streaking, place it at 28℃ for 2~3 days, pick a single colony with good growth and inoculate it into 10 mL of YPD liquid medium, and incubate it at 28℃ and 220 rpm for about 24 hours. Take 5 mL of the above culture to continue to expand the culture, inoculate it into 500 mL of YPD liquid medium, and incubate it at 28℃ and 220 rpm. When the OD600 of the shake flask seeds reaches 10, use it as the seed liquid for tanking.

[0036] Put 20 L of BSM medium into the fermenter, adjust the pH to 5.0 with ammonia water, sterilize at 121℃ for 30 min, and correct the dissolved oxygen to 0% after cooling. Set the temperature to 28℃, the ventilation volume to 20 L / min, and the tank pressure to 0.05 MPa. Add 87 mL of sterilized PTM1 solution to the culture medium, and adjust the pH of the culture medium to 5.0 again. Discard about 500 mL of the culture medium in the fermenter through the sampling port, so that the volume in the tank before inoculation is about 19.5 L. Pump the shake flask seeds into the fermenter, correct the dissolved oxygen to 100%, and set the speed to 300 rpm. Select "speed linkage" for the dissolved oxygen control mode, and maintain the dissolved oxygen above 30% by adjusting the speed (300~1000 rpm) and ventilation during fermentation.

[0037] When the dissolved oxygen increases suddenly and the rotation speed drops suddenly, the glycerol feed fermentation stage begins. The glycerol feed is added at a flow rate of 15 mL / h•L, and the parameters in the tank remain unchanged.

[0038] After 30 h of fermentation (wet weight reached about 242 g / L), the mixed feeding stage was entered, and glycerol feed and methanol feed were added simultaneously. At this time, the temperature was lowered to 24°C and the pH was adjusted to 5.85.

[0039] After 2 hours of mixed feed flow, the methanol feed period began, the dissolved oxygen-speed coupling was released, and the dissolved oxygen was coupled with the methanol feed. The coupling correlation was inversely correlated, and the critical value was 30%, that is, the feed was started when the dissolved oxygen was higher than the critical value, and stopped when the dissolved oxygen was lower than the critical value. The working cycle of the methanol feed was 10 s. The fermentation ended at 132 h and the tank was released. The fermentation product was centrifuged at 8000 rpm for 20 minutes, the supernatant was collected, and stored at -20°C, and the high-expression and low-O-glycosylation albumin was obtained, which was subsequently abbreviated as recombinant human albumin.

[0040] Red ginseng, wild grape seed and blueberry peel were crushed and passed through a 10-mesh sieve to obtain red ginseng powder, wild grape seed powder and blueberry peel powder. The powders were soaked in 75% ethanol at a solid-liquid ratio of 1:5 for 6 h, refluxed for 15 min at a water bath temperature, extracted 3 times, and the filtrates were combined and distilled to remove ethanol until there was no alcohol taste, thereby obtaining red ginseng extract, wild grape seed extract and blueberry peel extract. Red ginseng extract, wild grape seed extract and blueberry peel extract were suspended in purified water according to mass fraction to 100 mL to obtain a solution, the solution was evenly mixed to obtain a mixed solution, and the mixed solution was spray dried to obtain a mixed powder of red ginseng extract, wild grape seed extract and blueberry peel extract. The inlet air temperature of the spray drying was 140°C, the atomization pressure was 0.5 Mpa, and the feed rate was 500 mL / h.

[0041] Red ginseng extract, wild grape seed extract, blueberry peel extract (the extract uses the above mixed powder), glycerin, sodium alginate and carboxymethyl cellulose are placed in a 100 mL beaker according to their mass proportions, 50 mL of deionized water is added, and the mixture is stirred for 10 min at 60°C to obtain a uniform mixed solution. After the mixed solution is cooled to room temperature, it is slowly added to recombinant human albumin, the remainder is supplemented with deionized water, and the mixture is stirred at 65 rpm for 30 min to dissolve. The mixture is sterilized and filtered using a filter with a pore size of 0.22 µm to obtain human albumin anti-aging essence.

[0042] In this embodiment, 5 groups of human albumin anti-aging essences with different ingredients were prepared. The specific ingredients are as follows. In terms of mass parts, the total mass of the human albumin anti-aging essence is 100 parts, and the balance is supplemented with deionized water.

[0043] Group 1: 20 parts of recombinant human albumin, 5 parts of red ginseng extract, 3 parts of wild grape seed extract, 2 parts of blueberry peel extract, 25 parts of glycerol, 0.5 parts of sodium alginate, and 0.03 parts of carboxymethyl cellulose.

[0044] Group 2: 25 parts of recombinant human albumin, 10 parts of red ginseng extract, 6 parts of wild grape seed extract, 2.5 parts of blueberry peel extract, 30 parts of glycerol, 0.8 parts of sodium alginate, and 0.06 parts of carboxymethyl cellulose.

[0045] Group 3: 30 parts of recombinant human albumin, 15 parts of red ginseng extract, 10 parts of wild grape seed extract, 5 parts of blueberry peel extract, 40 parts of glycerol, 1.2 parts of sodium alginate, and 0.1 parts of carboxymethyl cellulose.

[0046] Group 4: 45 parts of recombinant human albumin, 12 parts of red ginseng extract, 8 parts of wild grape seed extract, 4.5 parts of blueberry peel extract, 25 parts of glycerol, 0.6 parts of sodium alginate, and 0.1 parts of carboxymethyl cellulose.

[0047] Group 5: 15 parts of recombinant human albumin, 15 parts of red ginseng extract, 8 parts of wild grape seed extract, 2.5 parts of blueberry peel extract, 35 parts of glycerol, 1.2 parts of sodium alginate, and 0.2 parts of carboxymethyl cellulose.

[0048] Comparative Example 1 On the basis of Group 2 in Example 1, 4 groups of comparative essences lacking recombinant human albumin, red ginseng extract, wild grape seed extract or blueberry peel extract were prepared. The specific ingredients are as follows, in parts by mass. The total mass of the comparative essence is 100 parts, and the remainder is supplemented with deionized water.

[0049] Group 6: 10 parts of red ginseng extract, 2.5 parts of blueberry peel extract, 6 parts of mountain grape seed extract, 30 parts of glycerin, 0.8 parts of sodium alginate, and 0.06 parts of carboxymethyl cellulose.

[0050] Group 7: 25 parts of recombinant human albumin, 2.5 parts of blueberry peel extract, 30 parts of glycerol, 0.8 parts of sodium alginate, and 0.06 parts of carboxymethyl cellulose.

[0051] Group 8: 25 parts of recombinant human albumin, 10 parts of red ginseng extract, 30 parts of glycerol, 0.8 parts of sodium alginate, and 0.06 parts of carboxymethyl cellulose.

[0052] Group 9: 25 parts of recombinant human albumin, 6 parts of Vitis vinifera seed extract, 30 parts of glycerol, 0.8 parts of sodium alginate, and 0.06 parts of carboxymethyl cellulose.

[0053] Test Case The human albumin anti-aging essence groups 1 to 5 obtained in Example 1 and the comparative essence groups 6 to 9 obtained in Comparative Example 1 were tested, and the commercially available liquid A (Angel's Color Bose Ginseng Anti-Wrinkle Firming Essence, item number TS202310-100ml) was used as group 10, and the commercially available liquid B (Olay Brightening Essence, item number: OL20231201) was used as group 11 for comparison.

[0054] In vitro antioxidant test (DPPH scavenging ability) Preparation of DPPH solution: Accurately weigh DPPH (20 mg), dissolve it in anhydrous ethanol and dilute it to a 500 mL brown volumetric flask to obtain a DPPH solution with a mass fraction of 0.004%. Store it away from light for later use.

[0055] Vitamin C was selected as the positive control, and the sample groups were experimental group (groups: 1-5), control group (groups 6-9) and commercial product group (groups: 10-11). The solution was prepared with a starting concentration of 1 mg / mL, and the concentration range was 0.002-1 mg / mL. The 96-well plate was divided into a sample group and a control group, with 3 replicates in each group. A blank control was set for both the sample group and the positive control. The blank control was anhydrous ethanol, which was added to the DPPH solution in an equal volume of 1:1. The 96-well plate was reacted in the dark at room temperature for 30 min, shaken and evenly mixed, and the absorbance OD value was measured at a wavelength of 517 nm. The half inhibition concentration (IC 50 ) values ​​(μg / mL), and the results are shown in Table 1. 50 The IC value is an important indicator to measure the strength of antioxidant activity. It indicates the concentration required for the antioxidant capacity to achieve half of the clearance effect. 50 The smaller the value, the stronger its antioxidant capacity. The calculation formula (1) for free radical scavenging rate is as follows: Free radical scavenging rate (%) = ( )×100%(1) In the formula: A1: measured OD value of the sample group; A2: measured OD value of the sample control group; A0: measured OD value of the blank group.

[0056] Table 1 Antioxidant test results

[0057] Note: Compared with the vitamin C group, P<0.05, * has significant difference.

[0058] As shown in Table 1, the DPPH scavenging activity of the experimental group (groups 1 to 5) and the control group (groups 6 to 9) was 12.6±0.29~22.8±0.45, which was better than vitamin C (25.7±0.15). 50 The IC values ​​(12.6±0.29~14.8±0.11) were compared with those of commercially available products (groups 10~11). 50 The values ​​of group 2 are close to or even lower than those of group 2 (14.4±0.35~15.0±0.35). 50 The value (12.6±0.29) was significantly lower than that of the commercially available product group.

[0059] At the same time, the DPPH scavenging ability of group 6 without recombinant human albumin was weaker than that of the experimental groups (groups 1-5), which further indicated that recombinant human albumin had good antioxidant capacity, which was derived from its abundant free thiol groups. The experimental data of the control groups (groups 6-9) were weaker than those of the experimental groups (groups 1-5), indicating that the four active ingredients of the human albumin anti-aging essence were indispensable.

[0060] Cell viability test Combined with the test results of the DPPH method, experimental groups (groups 1 to 5) were selected to carry out cell activity tests.

[0061] The CCK8 method was used to determine the effect of human albumin anti-aging essence on fibroblast viability: cells in the logarithmic growth phase were cultured at 5×10 3 The cells were inoculated at a density of 100 μL / 100 μL in a 96-well plate and incubated overnight at 37°C and 5% CO2. The cells were treated with groups 1 to 5 and cultured for 24 h. 10 μL of CCK-8 reagent was added to each well and incubated at 37°C for 1 h. The OD450nm value was measured and the cell viability was calculated. The OD450nm value of the experimental group was recorded as As, and the OD450nm value of the control group and the blank group was recorded as Ac and Ab. All samples were measured three times. Cell activity calculation was performed according to the following formula.

[0062] The experimental data are shown in Table 2.

[0063] Cell viability (%) = [(As-Ab) / (Ac-Ab)] × 100% As: absorbance of the experimental group (including cells, culture medium, CCK-8 solution and essence); Ac: absorbance of the control group (containing cells, culture medium, CCK-8 solution, but not essence); Ab: absorbance of blank group (containing culture medium and CCK-8 solution, but without cells or essence).

[0064] Table 2 Cell activity test results

[0065] Note: Compared with group 1, P<0.05, * has significant difference.

[0066] As shown in Table 2, the cell survival rates of fibroblasts cultured using groups 1 to 5 were all above 87%, indicating that the human albumin anti-aging essence was non-toxic to cells and had a proliferation-promoting effect on fibroblasts.

[0067] Hygiene index determination experiment According to the microbiological test method of the "Safety Technical Specifications for Cosmetics" (2015 edition), the total colony count, heat-resistant Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, mold and yeast in human albumin anti-aging essence (groups 1 to 5) were tested. The results of the hygiene indicators of human albumin anti-aging essence are shown in Table 3.

[0068] Table 3 Hygiene index measurement results

[0069] As shown in Table 3, the test results of various indicators of the hygiene of human albumin anti-aging essence are in compliance with the regulations.

[0070] Anti-wrinkle efficacy test According to the "Technical Specifications for Safety of Cosmetics" (2015 edition), 30 Chinese women aged 35 to 65 years old were selected, and all of them used the essence prepared by the experimental group (Group 2). The subjects used it once a day after cleansing their faces, applied it to the cheeks of the face, and gently massaged until absorbed. During the experiment, other anti-wrinkle skin care products were stopped. Skin tests were conducted at week 0, 2 weeks, and 4 weeks. Before the test, the facial skin was cleaned with clean water, and after cleaning, the subjects sat in a constant temperature and humidity room for 30 minutes. After 30 minutes, the facial skin anti-wrinkle efficacy test was conducted on the subjects participating in the experiment, and the control was selected as the control before and after. The skin wrinkle score, skin roughness, and skin elasticity test data of all subjects before using the product were used as the control group indicators, and the indicators of the test subjects after using the product for 2 weeks and 4 weeks were used as the experimental group indicators. The experimental results are shown in Table 4.

[0071] Table 4 Comparison of anti-aging indicators of face and cheek (X±s, n=30)

[0072] Before using the essence, the average skin roughness of 30 subjects was 3.01±0.25, which decreased to 2.63±0.15 after two weeks of use, and the average skin roughness was significantly improved; the skin elasticity R2 value and the skin elasticity Q1 value were 45.10±0.07 and 42.19±0.35 before use, respectively, and increased to 51.12±0.21 and 49.03±0.18 after two weeks of use, respectively, and the skin elasticity values ​​showed an upward trend. The test data show that the human albumin anti-aging essence prepared by the present invention can effectively improve skin elasticity and make the skin firm, thereby playing an anti-aging effect.

[0073] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A human albumin anti-aging essence, characterized in that: In parts by mass, it includes the following components: Human albumin 20-45 parts; 5-15 parts of red ginseng extract; 2.5-10 parts of Vitis vinifera seed extract; 1.5-5 parts of blueberry peel extract; 20~40 parts of moisturizer; Thickener 0.2~1.6 parts; Excipient 0.01~0.1 part; The balance was deionized water.

2. The human albumin anti-aging essence according to claim 1, characterized in that: The human albumin includes recombinant human albumin.

3. The human albumin anti-aging essence according to claim 2, characterized in that: The recombinant human albumin is an albumin with high expression and low O-glycosylation level.

4. The human albumin anti-aging essence according to claim 1, characterized in that: The red ginseng extract, the wild grape seed extract and the blueberry peel extract are obtained by extraction with 75% ethanol, filtration and distillation.

5. The human albumin anti-aging essence according to claim 1, characterized in that: The moisturizer includes at least one of glycerin, ethylhexylglycerin, 1,3-propylene glycol, 1,3-butylene glycol, 1,2-hexanediol and 1,2-pentanediol; the thickener includes at least one of sodium alginate, calcium alginate, potassium alginate, alginic acid and sodium lactate; and the excipient includes at least one of hydroxymethyl cellulose, hydroxyethyl cellulose, methyl cellulose and hydroxypropyl methyl cellulose.

6. A method for preparing the human albumin anti-aging essence according to any one of claims 1 to 5, characterized in that: The following steps are involved: Red ginseng extract, wild grape seed extract, blueberry peel extract, moisturizer, thickener, excipient and deionized water are stirred to prepare a mixed solution, the mixed solution is added to human albumin and deionized water is added to make up the balance, and the human albumin anti-aging essence is obtained by stirring, dissolving, sterilizing and filtering.

7. The preparation method according to claim 6, characterized in that: The temperature of the mixed solution is 50°C to 70°C for 5 to 15 minutes, and the mixed solution is added to human albumin after cooling; The stirring speed of the stirring dissolution is 50~80 rpm, the time is 20~45 min, and the pore size of the sterilization filtration is 0.22 µm.

8. The preparation method according to claim 6, characterized in that: Also includes red ginseng extract, mountain grape seed extract and blueberry peel extract preparation: Red ginseng, wild grape seed and blueberry peel were crushed and sieved to obtain red ginseng powder, wild grape seed powder and blueberry peel powder respectively. The red ginseng powder, wild grape seed powder and blueberry peel powder were respectively soaked in 75% ethanol and then refluxed for extraction. The filtrates were combined and the ethanol was distilled off until there was no alcohol taste to obtain red ginseng extract, wild grape seed extract and blueberry peel extract.

9. The preparation method according to claim 8, characterized in that: The soaking material-liquid ratio is 1:(5~10), the soaking time is 5~7h, and the reflux extraction is performed 2~4 times, with each reflux extraction lasting 10~20 min.

10. The preparation method according to claim 6, characterized in that: The red ginseng extract, wild grape seed extract and blueberry peel extract are respectively resuspended in water according to their mass fractions to prepare solutions, and the solutions are mixed and spray-dried to prepare a mixture for use. The inlet air temperature of the spray drying is 120-160° C., the atomization pressure is 0.1-1.0 Mpa, and the feed rate is 200-800 mL / h.

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