Moisturizing anti-wrinkle essence and preparation method thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly discloses a moisturizing anti-wrinkle essence and a preparation method thereof, the moisturizing anti-wrinkle essence comprises the following components by mass: 4-8% of polyol, 0.2-0.4% of a thickener, 0.1-0.2% of EDTA-disodium, 0.2-0.4% of arginine, 0.1-2% of a preservative, 1-10% of rose hydrosol, 20-35% of a pitaya peel fermentation enzymolysis nanofiltration extract, and the balance of deionized water; the pitaya peel fermentation enzymolysis nanofiltration extracting solution contains anthocyanin, water-soluble vitamins, polypeptides, amino acids, monosaccharides and oligosaccharides. According to the pitaya peel fermentation enzymolysis nanofiltration extracting solution, nutrient substances of pitaya peel can be extracted to the maximum extent, so that vitamin C with the relative molecular mass of 176 and anthocyanin with the relative molecular mass of 287 are completely dissolved out under the condition that the biological activity is not affected.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a moisturizing and anti-wrinkle essence and a preparation method thereof. Background Art

[0002] Essence is a skin care product mainly used for facial care. With its high concentration and high efficiency, it has become an indispensable part of the skin care process for many people.

[0003] With the rapid development of the cosmetics industry and the improvement of consumers' beauty and skin care concepts, more and more consumers are beginning to pursue efficacy. As the mainstream consumer group of cosmetics, consumers aged 25-35 are inevitably interested in anti-wrinkle and anti-aging cosmetics. However, many anti-wrinkle cosmetics on the market often have low efficacy and high irritation because the anti-wrinkle raw materials added have large molecular weight, low absorption rate, and are not natural enough. Therefore, it is imperative to develop a greener, more natural and effective anti-wrinkle essence. Summary of the invention

[0004] The invention provides a moisturizing and anti-wrinkle essence and a preparation method thereof, which has excellent moisturizing and anti-oxidation effects.

[0005] The present invention solves the technical problem by adopting the following technical solutions: A moisturizing and anti-wrinkle essence, comprising the following components in percentage by mass: 4-8% polyol, 0.2-0.4% thickener, 0.1-0.2% EDTA-disodium, 0.2-0.4% arginine, 0.1-2% preservative, 1-10% rose hydrosol, 20-35% pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, and the balance deionized water; The pitaya peel fermentation enzymatic hydrolysis nanofiltration extract contains anthocyanidins, water-soluble vitamins, polypeptides, amino acids, monosaccharides and oligosaccharides.

[0006] As a preferred embodiment of the present invention, the method for preparing the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is: (1) Washing fresh dragon fruit peel, soaking it in deionized water, cold pressing it, and filtering it to obtain a filtrate; (2) adding yeast, Bacillus subtilis, and Lactobacillus to the filtrate for anaerobic fermentation to obtain a fermentation liquid; (3) Adding the complex enzyme to the fermentation broth, performing enzymatic hydrolysis, first filtering with a nanofiltration membrane with a molecular weight cutoff of 1000 Da, and then filtering with a nanofiltration membrane with a molecular weight cutoff of 100 Da, to obtain a pitaya peel fermentation enzymatic hydrolysis nanofiltration extract.

[0007] The relative molecular weight of anthocyanidins, water-soluble vitamins, polypeptides, amino acids, monosaccharides and oligosaccharides in the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract of the present invention is 100-1000 Da, which is more easily absorbed by the skin and can achieve good moisturizing and anti-wrinkle effects.

[0008] The moisturizing and anti-wrinkle essence of the present invention is added with a pitaya peel fermentation enzymatic nanofiltration extract obtained by fermentation, enzymatic hydrolysis and nanofiltration separation. Fermentation can activate the maximum biological activity of pitaya peel and improve the effective component dissolution rate and nutrient content of pitaya peel; enzymatic hydrolysis can decompose the macromolecular protein in pitaya peel into small molecular polypeptides and amino acids, and degrade the polysaccharide substances constituting the cell wall into monosaccharides and oligosaccharides, which not only avoids the possibility of macromolecular protein and polysaccharide as foreign matter to cause irritation to the skin, but also converts them into small molecular polypeptides, amino acids, monosaccharides and oligosaccharides that are beneficial to the skin to absorb; nanofiltration separation can intercept components with a relative molecular weight of 100 to 1000 Da, and the extract within this relative molecular weight range is more easily absorbed by the skin and filters the microorganisms brought by the fermentation process and raw materials, so that the extract finally obtained is safer and more effective. The pitaya peel fermentation enzymatic nanofiltration extract after fermentation enzymatic hydrolysis and nanofiltration separation treatment contains rich anthocyanins, water-soluble vitamins, small molecular polypeptides, amino acids, monosaccharides and oligosaccharides.

[0009] The pitaya peel fermentation enzymatic hydrolysis nanofiltration extract of the present invention can extract the nutrients of the pitaya peel to the maximum extent, so that vitamin C with a relative molecular mass of 176 and anthocyanin with a relative molecular mass of 287 are completely dissolved without affecting the biological activity.

[0010] As a preferred embodiment of the present invention, the cold pressing temperature is 18-22° C. and the pressure is 18-22 MPa.

[0011] As a preferred embodiment of the present invention, the fermentation temperature is 26-30° C., and the fermentation time is 6-8 days.

[0012] As a preferred embodiment of the present invention, the mass ratio of the yeast, Bacillus subtilis, Lactobacillus and the filtrate is (2.8-3.2):(1.8-2.2):(0.8-1.2):100.

[0013] As a preferred embodiment of the present invention, the complex enzyme includes endopeptidase, cellulase, hemicellulase and glucoamylase, and the mass ratio of the endopeptidase, cellulase, hemicellulase, glucoamylase and fermentation broth is (0.001~0.005):(0.001~0.005):(0.001~0.005):(0.001~0.005):1.

[0014] As a preferred embodiment of the present invention, the polyol includes at least one of glycerol, butylene glycol, sorbitol, xylitol, glyceryl polyether-26, hydroxyethyl urea, erythritol, and dipropylene glycol.

[0015] As a preferred embodiment of the present invention, the thickener comprises acrylic acid (ester) / C 10～30 At least one of alkyl acrylate crosspolymer, carbomer, and acrylates / vinyl isodecanoate crosspolymer.

[0016] As a preferred embodiment of the present invention, the preservative includes at least one of phenoxyethanol, p-hydroxyacetophenone, 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, ethylhexylglycerin, sodium benzoate, and caprylhydroxamic acid.

[0017] The present invention also provides a method for preparing a moisturizing and anti-wrinkle essence, comprising the following steps: Mix the polyol and thickener evenly, then add disodium EDTA and deionized water, stir and heat to 75-85°C and then homogenize, keep warm and stir for 15-25 minutes, then add arginine and stir evenly, when the temperature drops to 40-45°C, add preservatives, rose hydrosol and pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, mix evenly to obtain a moisturizing and anti-wrinkle essence.

[0018] Beneficial effects of the present invention: The moisturizing and anti-wrinkle essence of the present invention is added with a pitaya peel fermentation enzymatic nanofiltration extract obtained by fermentation, enzymatic hydrolysis and nanofiltration separation. Fermentation can activate the maximum biological activity of pitaya peel and improve the dissolution rate of effective ingredients and the content of nutrients in pitaya peel; enzymatic hydrolysis can decompose the macromolecular protein in pitaya peel into small molecular polypeptides and amino acids, and degrade the polysaccharide substances constituting the cell wall into monosaccharides and oligosaccharides, which not only avoids the possibility of macromolecular protein and polysaccharide as foreign matter causing irritation to the skin, but also converts them into small molecular polypeptides, amino acids, monosaccharides and oligosaccharides that are beneficial to the skin to absorb; nanofiltration separation can intercept components with a relative molecular weight of 100 to 1000 Da. The extract within this relative molecular weight range is more easily absorbed by the skin and filters the microorganisms brought by the fermentation process and raw materials, making the final extract safer and more effective. The pitaya peel fermentation enzymatic nanofiltration extract after fermentation enzymatic hydrolysis and nanofiltration separation treatment is rich in anthocyanins, water-soluble vitamins, small molecular polypeptides, amino acids, monosaccharides and oligosaccharides. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] In the present invention, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0021] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0022] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0023] Among them, the yeast number is BNCC140102; the Bacillus subtilis number is BNCC195731; and the Lactobacillus number is BNCC193194. All of the above microorganisms are provided by Beina Biotech Henan Industrial Microbial Strain Engineering Technology Research Center.

[0024] Example 1 A moisturizing and anti-wrinkle essence, comprising the following components in percentage by mass: 0.3% acrylic acid (ester) / C 10～30 Alkyl acrylate crosspolymer (Carbopol Ultrez 21, the same below), 3% glycerin, 3% butylene glycol, 0.1% disodium EDTA, 0.25% arginine, 3% rose hydrosol, 25% pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, 0.4% phenoxyethanol, 0.1% ethylhexylglycerin, and the balance deionized water.

[0025] The preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is: (1) Wash the fresh dragon fruit peel and soak it in deionized water, cold press it with a cold residue machine at 20°C and a pressure of 20 MPa for 20 min, and filter it with a 1 mm filter cloth to obtain a filtrate; (2) adding yeast, Bacillus subtilis, and Lactobacillus to the filtrate, and performing anaerobically fermentation at 28° C. for 7 days to obtain a fermentation liquid; The mass ratio of the yeast, Bacillus subtilis, lactobacillus and filtrate is 3:2:1:100.

[0026] (3) The complex enzyme was added to the fermentation broth, and enzymatic hydrolysis was performed at 50°C for 4 h. The broth was first filtered with a nanofiltration membrane with a molecular weight cutoff of 1000 Da, and then filtered with a nanofiltration membrane with a molecular weight cutoff of 100 Da to obtain a pitaya peel fermentation enzymatic hydrolysis nanofiltration extract.

[0027] The complex enzyme comprises endopeptidase, cellulase, hemicellulase and glucoamylase, and the mass ratio of the endopeptidase, cellulase, hemicellulase, glucoamylase and fermentation liquid is 0.003:0.003:0.003:0.003:1.

[0028] The method for preparing the moisturizing and anti-wrinkle essence comprises the following steps: Glycerin, butylene glycol, acrylic acid (esters) / C 10～30 The cross-linked polymer of alkyl acrylate is mixed evenly, and then disodium EDTA and deionized water are added, and the mixture is heated to 80°C with stirring and homogenized. The mixture is stirred for 20 minutes at a temperature keeping the mixture warm and adding arginine, and the mixture is stirred evenly. When the temperature is lowered to 45°C, phenoxyethanol, ethylhexylglycerin, rose hydrosol and pitaya peel fermentation enzymatic hydrolysis nanofiltration extract are added, and the mixture is mixed evenly to obtain a moisturizing and anti-wrinkle essence.

[0029] Example 2 The difference between Example 2 and Example 1 is that the amount of raw materials is different, and the other aspects are the same.

[0030] A moisturizing and anti-wrinkle essence comprises the following components in percentage by mass: 0.35% carbomer 980, 3% glycerol, 3% butylene glycol, 0.15% disodium EDTA, 0.3% arginine, 5% rose hydrosol, 30% pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, 0.4% phenoxyethanol, 0.1% ethylhexylglycerin, and the balance deionized water.

[0031] Example 3 The difference between Example 3 and Example 1 is that the amount of raw materials is different, and everything else is the same.

[0032] A moisturizing and anti-wrinkle essence, comprising the following components in percentage by mass: 0.3% acrylic acid (ester) / C 10～30 Alkyl acrylate crosspolymer, 4% glycerin, 4% butylene glycol, 0.12% disodium EDTA, 0.25% arginine, 5% rose hydrosol, 35% pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, 0.4% phenoxyethanol, 0.1% ethylhexylglycerin, and the balance deionized water.

[0033] Example 4 The difference between Example 4 and Example 1 is that the amount of raw materials is different, and everything else is the same.

[0034] A moisturizing and anti-wrinkle essence, comprising the following components in percentage by mass: 0.3% acrylic acid (ester) / C 10～30 Alkyl acrylate crosspolymer, 4% glycerin, 3% butylene glycol, 0.12% disodium EDTA, 0.25% arginine, 3% rose hydrosol, 28% pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, 0.4% phenoxyethanol, 0.1% ethylhexylglycerin, and the balance deionized water.

[0035] Example 5 The difference between Example 5 and Example 1 is that the amount of raw materials is different, and everything else is the same.

[0036] A moisturizing and anti-wrinkle essence comprises the following components in percentage by mass: 0.32% carbomer 980, 5% glycerol, 3% glycerol polyether-26, 0.15% disodium EDTA, 0.28% arginine, 5% rose hydrosol, 25% pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, 0.4% phenoxyethanol, 0.1% ethylhexylglycerin, and the balance deionized water.

[0037] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is different, and the other aspects are the same.

[0038] The preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract described in this comparative example is: (1) Wash the fresh dragon fruit peel and soak it in deionized water, cold press it with a cold residue machine at 20°C and a pressure of 20 MPa for 20 min, and filter it with a 1 mm filter cloth to obtain a filtrate; (2) adding lactobacillus to the filtrate and performing anaerobically fermentation at 28° C. for 7 days to obtain a fermentation liquid; The mass ratio of the lactobacillus to the filtrate is 6:100.

[0039] (3) The complex enzyme was added to the fermentation broth, and enzymatic hydrolysis was performed at 50°C for 4 h. The broth was first filtered with a nanofiltration membrane with a molecular weight cutoff of 1000 Da, and then filtered with a nanofiltration membrane with a molecular weight cutoff of 100 Da to obtain a pitaya peel fermentation enzymatic hydrolysis nanofiltration extract.

[0040] The complex enzyme comprises endopeptidase, cellulase, hemicellulase and glucoamylase, and the mass ratio of the endopeptidase, cellulase, hemicellulase, glucoamylase and fermentation liquid is 0.003:0.003:0.003:0.003:1.

[0041] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is different, and the other aspects are the same.

[0042] The preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is: (1) Wash the fresh dragon fruit peel and soak it in deionized water, cold press it with a cold residue machine at 20°C and a pressure of 20 MPa for 20 min, and filter it with a 1 mm filter cloth to obtain a filtrate; (2) adding yeast to the filtrate and performing anaerobically fermentation at 28° C. for 7 days to obtain a fermentation liquid; The mass ratio of the yeast to the filtrate is 6:100.

[0043] (3) The complex enzyme was added to the fermentation broth, and enzymatic hydrolysis was performed at 50°C for 4 h. The broth was first filtered with a nanofiltration membrane with a molecular weight cutoff of 1000 Da, and then filtered with a nanofiltration membrane with a molecular weight cutoff of 100 Da to obtain a pitaya peel fermentation enzymatic hydrolysis nanofiltration extract.

[0044] The complex enzyme comprises endopeptidase, cellulase, hemicellulase and glucoamylase, and the mass ratio of the endopeptidase, cellulase, hemicellulase, glucoamylase and fermentation liquid is 0.003:0.003:0.003:0.003:1.

[0045] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that the preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is different, and the other aspects are the same.

[0046] The preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is: (1) Wash the fresh dragon fruit peel and soak it in deionized water, cold press it with a cold residue machine at 20°C and a pressure of 20 MPa for 20 min, and filter it with a 1 mm filter cloth to obtain a filtrate; (2) adding yeast, Bacillus subtilis, and Lactobacillus to the filtrate, and performing anaerobically fermentation at 28° C. for 7 days to obtain a fermentation liquid; The mass ratio of the yeast, Bacillus subtilis, lactobacillus and filtrate is 3:2:1:100.

[0047] (3) The complex enzyme was added to the fermentation broth, and enzymatic hydrolysis was performed at 50°C for 4 h. The broth was first filtered with a nanofiltration membrane with a molecular weight cutoff of 1000 Da, and then filtered with a nanofiltration membrane with a molecular weight cutoff of 100 Da to obtain a pitaya peel fermentation enzymatic hydrolysis nanofiltration extract.

[0048] The complex enzyme comprises cellulase and pectinase, and the mass ratio of the cellulase, pectinase and fermentation liquid is 0.006:0.006:1.

[0049] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that pitaya peel enzymatic hydrolysis nanofiltration extract is used to replace pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, and the other contents are the same.

[0050] The preparation method of the pitaya peel enzyme hydrolysis nanofiltration extract is: (1) Wash the fresh dragon fruit peel and soak it in deionized water, cold press it with a cold residue machine at 20°C and a pressure of 20 MPa for 20 min, and filter it with a 1 mm filter cloth to obtain a filtrate; (2) Add the complex enzyme to the filtrate, perform enzymatic hydrolysis at 50°C for 4 h, first filter with a nanofiltration membrane with a molecular weight cutoff of 1000 Da, and then filter with a nanofiltration membrane with a molecular weight cutoff of 100 Da to obtain the enzymatic hydrolysis nanofiltration extract of pitaya peel.

[0051] The complex enzyme comprises endopeptidase, cellulase, hemicellulase and glucoamylase, and the mass ratio of the endopeptidase, cellulase, hemicellulase, glucoamylase and fermentation liquid is 0.003:0.003:0.003:0.003:1.

[0052] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that pitaya peel fermentation nanofiltration extract is used to replace pitaya peel fermentation nanofiltration extract, and the other contents are the same.

[0053] The preparation method of the pitaya peel fermentation nanofiltration extract is: (1) Wash the fresh dragon fruit peel and soak it in deionized water, cold press it with a cold residue machine at 20°C and a pressure of 20 MPa for 20 min, and filter it with a 1 mm filter cloth to obtain a filtrate; (2) adding yeast, Bacillus subtilis, and Lactobacillus to the filtrate, and performing anaerobically fermentation at 28° C. for 7 days to obtain a fermentation liquid; (3) The fermentation broth was first filtered through a nanofiltration membrane with a molecular weight cutoff of 1000 Da, and then filtered through a nanofiltration membrane with a molecular weight cutoff of 100 Da to obtain a pitaya peel fermentation nanofiltration extract.

[0054] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that the preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is different, and the others are the same.

[0055] The preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is: (1) Wash the fresh dragon fruit peel and soak it in deionized water, cold press it with a cold residue machine at 20°C and a pressure of 20 MPa for 20 min, and filter it with a 1 mm filter cloth to obtain a filtrate; (2) adding yeast, Bacillus subtilis, and Lactobacillus to the filtrate, and performing anaerobically fermentation at 28° C. for 7 days to obtain a fermentation liquid; The mass ratio of the yeast, Bacillus subtilis, lactobacillus and filtrate is 3:2:1:100.

[0056] (3) The complex enzyme was added to the fermentation broth, and enzymatic hydrolysis was performed at 50°C for 4 h. The broth was first filtered with a nanofiltration membrane with a molecular weight cutoff of 5000 Da, and then filtered with a nanofiltration membrane with a molecular weight cutoff of 3000 Da to obtain the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract.

[0057] The complex enzyme comprises endopeptidase, cellulase, hemicellulase and glucoamylase, and the mass ratio of the endopeptidase, cellulase, hemicellulase, glucoamylase and fermentation liquid is 0.003:0.003:0.003:0.003:1.

[0058] Test Case 1. Skin feel test: Test subjects: 15 volunteers (male:female ratio is 7:8), aged 20 to 35 years old, in good health.

[0059] Test conditions: 1) Indoor environment; 2) No strong sunlight or direct light; 3) Ambient temperature 25±2℃, ambient humidity 40%~60%.

[0060] Test method: The essence of Example 1; volunteers first washed their faces with warm water, then used the essence of Example 1 (3 drops), and evaluated the skin feel after resting for 0.5 hours.

[0061] Table 1

[0062] It can be seen that the essence of the present invention has a good skin feel.

[0063] 2. Comparative test of skin moisture content: Test subjects: 110 volunteers (male to female ratio of 1:1), aged 20 to 35 years old, healthy. The 110 volunteers were randomly divided into 11 groups, 10 people in each group, and the products of the test examples and comparative examples were used in the morning and evening (3 drops each time), and the test area was a 2cm×3cm rectangular area selected from the same part of the forehead.

[0064] Test conditions: 1) Indoor environment; 2) No strong sunlight or direct light; 3) Ambient temperature 25±2℃, ambient humidity 40%~60%.

[0065] The following tests were conducted for 24 hours: the skin moisture tester MDD4-Corneometer CM825 was used for testing, and the average was taken as the skin moisture content before use (recorded as A0); the volunteers then washed their faces with clean water, and 24 hours later, the skin moisture tester MDD4-Corneometer CM825 was used for testing, and the average was taken as the skin moisture content after 24 hours of use (recorded as A1), and the skin moisture content change rate was calculated = [(A1-A0) / A0] 100%. The test results are shown in Table 2.

[0066] 3. The essence prepared in the examples and comparative examples was diluted with deionized water to 1 mg / mL as the sample solution. Vitamin C was used as the control group. 2.00×10 -4 Add 3 mL of 1 mol / L DPPH·anhydrous ethanol solution and 3 mL of sample solution into a test tube, shake well, and place it away from light for 30 min. Then measure the absorbance Ai of the mixed solution at 517 nm. At the same time, measure the absorbance A0 of 3 mL of pure water mixed with 3 mL of DPPH·anhydrous ethanol solution and the absorbance Aj of 3 mL of sample solution mixed with 3 mL of anhydrous ethanol. Calculate the DPPH· clearance rate (%) according to the formula.

[0067] DPPH· removal rate (%) = [1-(Ai-Aj) / A0] × 100% Table 2

[0068] It can be seen from Table 2 that the moisturizing and anti-wrinkle essence of the present invention has excellent moisturizing and anti-oxidation effects.

[0069] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A moisturizing and anti-wrinkle essence, characterized in that: The invention comprises the following components in percentage by weight: 4-8% polyol, 0.2-0.4% thickener, 0.1-0.2% disodium EDTA, 0.2-0.4% arginine, 0.1-2% preservative, 1-10% rose water, 20-35% pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, and the balance deionized water; The pitaya peel fermentation enzymatic hydrolysis nanofiltration extract contains anthocyanidins, water-soluble vitamins, polypeptides, amino acids, monosaccharides and oligosaccharides.

2. The moisturizing and anti-wrinkle essence according to claim 1, characterized in that: The preparation method of the pitaya peel fermentation enzymatic hydrolysis nanofiltration extract is: (1) Washing fresh dragon fruit peel, soaking it in deionized water, cold pressing it, and filtering it to obtain a filtrate; (2) adding yeast, Bacillus subtilis, and Lactobacillus to the filtrate for anaerobic fermentation to obtain a fermentation liquid; (3) Adding the complex enzyme to the fermentation broth, performing enzymatic hydrolysis, first filtering with a nanofiltration membrane with a molecular weight cutoff of 1000 Da, and then filtering with a nanofiltration membrane with a molecular weight cutoff of 100 Da, to obtain a pitaya peel fermentation enzymatic hydrolysis nanofiltration extract.

3. The moisturizing and anti-wrinkle essence according to claim 2, characterized in that: The cold pressing temperature is 18-22° C. and the pressure is 18-22 MPa.

4. The moisturizing and anti-wrinkle essence according to claim 2, characterized in that: The fermentation temperature is 26-30° C., and the fermentation time is 6-8 days.

5. The moisturizing and anti-wrinkle essence according to claim 2, characterized in that: The mass ratio of the yeast, Bacillus subtilis, lactobacillus and filtrate is (2.8-3.2):(1.8-2.2):(0.8-1.2):

100.

6. The moisturizing and anti-wrinkle essence according to claim 2, characterized in that: The complex enzyme comprises endopeptidase, cellulase, hemicellulase and glucoamylase, and the mass ratio of the endopeptidase, cellulase, hemicellulase, glucoamylase and fermentation broth is (0.001-0.005):(0.001-0.005):(0.001-0.005):(0.001-0.005):

1.

7. The moisturizing and anti-wrinkle essence according to claim 1, characterized in that: The polyol includes at least one of glycerol, butylene glycol, sorbitol, xylitol, glyceryl polyether-26, hydroxyethyl urea, erythritol, and dipropylene glycol.

8. The moisturizing and anti-wrinkle essence according to claim 1, characterized in that: The thickener includes acrylic acid (ester) / C 10～30 At least one of alkyl acrylate crosspolymer, carbomer, and acrylates / vinyl isodecanoate crosspolymer.

9. The moisturizing and anti-wrinkle essence according to claim 1, characterized in that: The preservative comprises at least one of phenoxyethanol, p-hydroxyacetophenone, 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, ethylhexylglycerin, sodium benzoate and caprylhydroxamic acid.

10. The method for preparing the moisturizing and anti-wrinkle essence according to any one of claims 1 to 9, characterized in that: The following steps are involved: Mix the polyol and thickener evenly, then add disodium EDTA and deionized water, stir and heat to 75-85°C and then homogenize, keep warm and stir for 15-25 minutes, then add arginine and stir evenly, when the temperature drops to 40-45°C, add preservatives, rose hydrosol and pitaya peel fermentation enzymatic hydrolysis nanofiltration extract, mix evenly to obtain a moisturizing and anti-wrinkle essence.

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