COSMETIC COMPOSITION FOR RELEASING THERMAL STIMULATION CONTAINING COMPLEX EXTRACT OF Ophiopogon

Abstract

The present invention relates to a cosmetic composition for relieving thermal stimulation containing a composite extract of ophiopogon root, ginseng, and Schisandra chinensis. The extract can achieve the efficacy of improving skin inflammation by inhibiting epoxidase-2, which is an inflammatory factor expressed by thermal stimulation. In addition, it is possible to achieve excellent wrinkle improvement and elasticity improvement effects by inhibiting matrix metalloproteinase-1 and restoring the expression of collagen type 1, fibrin-1, and elastin, and it is also possible to induce skin moisturizing by enhancing the expression of ceramide synthetase 3 or bleomycin hydrolase. Therefore, the composition can be used as a cosmetic composition for relieving menopause heat sensation or heat sensation caused by stimulation of a large amount of ultraviolet rays or skin heat injury after laser treatment.

Description

Technical Field

[0001] The invention relates to a cosmetic composition for relieving heat stimulation containing a composite extract of ophiopogon japonicus, ginseng and schisandra chinensis.

[0002] Preferably, the present invention relates to a cosmetic composition containing a composite extract extracted by using negative ion water after puffing Ophiopogon japonicus, ginseng and Schisandra chinensis, which can achieve the effects of improving skin inflammation, wrinkles, elasticity and skin moisturizing by alleviating various thermal stimuli generated on the skin. Background Art

[0003] Skin aging is divided into intrinsic aging that occurs with the passage of time and exogenous aging that occurs due to various external stresses including heat and ultraviolet rays. When the main factor of exogenous aging is ultraviolet rays, it is also called photoaging.

[0004] It is known that the ideal temperature of the skin is 30-31°C below body temperature. When a normal-temperature person is exposed to strong sunlight for 30 minutes, the skin temperature can rise to a maximum of 43°C. When the skin temperature rises, enzymes that break down collagen, the main component of the skin, are activated, which promotes skin damage and aging, and further leads to the formation of wrinkles. In addition, there are reports that oxidative stress can also interfere with skin aging when heat is increased.

[0005] In addition, it is known that facial flushing is one of the symptoms of menopause among skin problems. It is a symptom that the skin color becomes red due to sensitivity to sunlight and heat. When menopausal symptoms occur, the body temperature cannot be effectively regulated due to hormonal imbalance, and it also causes thermal stimulation to skin cells. The thermal stimulation mentioned above will cause the skin temperature to rise, thereby accelerating the aging of the skin.

[0006] The purpose of using cosmetics is to purify people's skin or show the aesthetic function of charming charm and the defensive function of protecting the skin from the external harmful environment. In recent years, a large number of functional cosmetics for alleviating various thermal stimulations to the face as described above have been developed. In addition, in order to achieve the effect of improving skin conditions such as whitening or hair removal, research activities related to skin improvement products that can alleviate thermal stimulation after laser treatment are also being actively carried out. Heat is a form of energy that can be transmitted in various ways such as direct contact heat conduction, circulation-convection and ultraviolet rays. However, although the thermal stimulation inside and outside the body as described above belongs to a different source of stimulation from light sources such as ultraviolet rays, very effective substances have not yet been found. Therefore, although thermal aging (thermal skin aging) and photoaging (photoaging) are similar in that the skin temperature rises because of the action of heat energy on the skin, it cannot be considered that the photoaging that occurs when directly exposed to light (especially sunlight) is equivalent to thermal aging (thermal skin aging) that occurs through various transmission methods. Therefore, it is necessary to explore substances that can improve direct thermal stimulation in a wider range, and it is also necessary to confirm it with natural substances that can further alleviate skin irritation as the center.

[0007] Prior art literature

[0008] Patent Literature

[0009] Korean registered patent No. 10-1736979 (Title of invention: Skin topical composition containing black plum extract, applicant: Amorepacific Co., Ltd., registration date: May 11, 2017)

[0010] Korean registered patent No. 10-1367423 (Title of invention: Pharmaceutical composition for skin improvement, cosmetic composition and its manufacturing method, applicant: Wonkwang University Industry-Academic Cooperation Group, registration date: February 19, 2014) Summary of the invention

[0011] The object of the present invention is to provide a cosmetic composition for relieving heat stimulation containing a composite extract of Radix Ophiopogonis, Radix Ginseng and Schisandrae Chinensis.

[0012] Preferably, the object of the present invention is to provide a cosmetic composition containing a composite extract extracted by using negative ion water after puffing Ophiopogon japonicus, ginseng and Schisandra chinensis, so as to achieve the effects of improving skin inflammation, wrinkles, elasticity and skin moisturizing by reducing various thermal stimuli generated on the skin.

[0013] The invention relates to a cosmetic composition for relieving heat stimulation, comprising a composite extract obtained by extracting ophiopogon japonicus, ginseng and schisandra chinensis puffed product using negative ion water.

[0014] The extraction condition of the negative ion water may be performed at 40 to 60° C. for 3 to 8 hours.

[0015] The present invention is characterized in that the thermal stimulation is heating stimulation of skin cells including facial flushing, menopausal thermal stimulation and laser thermal stimulation.

[0016] The extract relates to a cosmetic composition having skin inflammation improving effect, wrinkle improving effect, elasticity improving effect or skin moisturizing effect induced by thermal stimulation.

[0017] Preferably, the present invention is characterized in that skin inflammation is relieved by inhibiting the expression of inflammatory factor cyclooxygenase-2 (COX-2). In addition, the extract can improve skin wrinkles by inhibiting the expression of matrix metalloproteinase-1 (MMP-1) increased due to thermal stimulation. In another form, the extract can enhance skin elasticity by increasing the expression of collagen type I, fibrillin-1 or elastin decreased due to thermal stimulation.

[0018] Preferably, the extract can enhance the skin moisturizing effect by increasing the protein expression of ceramide synthase-3 (CERS3) or bleomycin hydrolase (BLMH).

[0019] Next, the present invention will be described in detail.

[0020] The Radix Ophiopogonis, Radix Ginseng and Fructus Schisandrae Chinensis can be in the range of 5 to 14 kg / cm 2 The expansion is carried out under the conditions of pressure of 100 to 150°C and temperature of 110 to 150°C.

[0021] In addition, it is suitable to extract the expanded Ophiopogon japonicus, Ginseng and Schisandra chinensis using negative ion water. The negative ion water can be acidic water with a pH of 3.5 to 6.5 produced by the stimulation of tourmaline and the electrolysis of pure water.

[0022] Regarding negative ion water, preferably, when the tourmaline raw stone or powder contacts water, the water is electrolyzed by instantaneous discharge, thereby decomposing water molecules (H2O) into hydrogen ions (H + ) and hydroxide (OH- ), at this time, the hydrogen ions will be attracted to the negative electrode and will be neutralized by combining with the electrons released from it, so that hydrogen (H2) will be volatilized into the air, and the hydroxide will combine with the surrounding water molecules and convert into hydrated hydroxyl ions (H3O2 - ) and remain, thereby generating negative ion water containing negative ions.

[0023] The extract can reduce the gene expression of cyclooxygenase-2 (COX-2), an inflammatory factor, to 60-70% at 100 to 300 μg / Ml compared with the heat stimulation treatment group.

[0024] The extract can reduce the gene expression of matrix metalloproteinase-1 (MMP-1) to 80-90% compared with the heat stimulation treatment group at 300 to 500 μg / Ml.

[0025] In addition, the extract can enhance the expression of collagen type I gene to 130-150%, enhance the expression of fibrillin-1 gene to 180-200% and enhance the expression of elastin gene to 230-250% compared with the heat stimulation treatment group at 300-500 μg / Ml.

[0026] In addition, the extract can enhance the expression of ceramide synthase-3 (CERS3) protein to 150-160% and enhance the expression of bleomycin hydrolase (BLMH) to 120-130% at 300 μg / Ml.

[0027] As the negative ion water used in the preparation of the extract, 1 to 40 times the volume (1 to 40 liters based on 1 kg) or 1 to 40 times the weight can be used based on the mixed weight of the expanded Ophiopogon japonicus, ginseng and Schisandra chinensis, preferably 5 to 40 times the volume or 5 to 40 times the weight can be used. The extraction condition of the extract can be performed at 40 to 60° C. for 3 to 24 hours. The process can be repeated 1 to 4 times.

[0028] As the extraction equipment for the extract, a general extraction equipment, an ultrasonic pulverization extractor or a fractionator can be used. The extract produced by the above method can be dried with hot air, dried under reduced pressure or freeze-dried to remove the solvent.

[0029] The extract can be used after fractionation or purification according to a general method by using, alone or in appropriate combination, known methods used in the separation and extraction of plant components, such as extraction using an organic solvent (such as ethanol, ether and acetone), partitioning between hexane and water, and using column chromatography.

[0030] The chromatography method can be selected from silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium pressure liquid chromatography, thin layer chromatography (TLC), silica gel vacuum liquid chromatography and high performance liquid chromatography.

[0031] The dosage form of the cosmetic composition can be manufactured into any dosage form commonly used in related industries, and can be selected from essence, skin care lotion, emulsion, facial mask, hand cream, foot cream, lip balm, lipstick, eye shadow, eyeliner, eyebrow pencil, blush, highlighter, ordinary lotion, skin care water, face cream, essence, beauty soap, skin softening lotion, medicinal lotion, shower gel, cleansing foam, cleansing milk, gel, makeup remover oil, cleansing cream, shampoo, conditioner, hair cream, hair conditioner, cleansing tissue and makeup remover.

[0032] Specifically, when the dosage form of the cosmetic composition according to the present invention is a paste, cream or gel, as a carrier component, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicon, bentonite, silicon dioxide, talc or zinc oxide can be used. When the dosage form of the cosmetic composition according to the present invention is a powder or spray, as a carrier component, lactose, talc, silicon dioxide, aluminum hydroxide, calcium silicate or polyamide powder can be used, especially in the case of a spray, a propellant such as chlorofluorocarbon, propane or dimethyl ether can be added. When the dosage form of the cosmetic composition according to the present invention is a solution or an emulsion, a solvent, a solvating agent or an emulsifier can be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester can be used. In the case where the dosage form of the cosmetic composition according to the present invention is a suspension, as carrier components, liquid diluents such as water and ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol and polyoxyethylene sorbitol esters and polyoxyethylene sorbitol esters, microcrystalline cellulose, aluminum hydroxide, bentonite, agar or tragacanth gum, etc. can be used. In the case where the dosage form of the cosmetic composition according to the present invention is a detergent containing a surfactant, as carrier components, fatty alcohol sulfates, fatty alcohol ether sulfates, sulfosuccinic acid monoesters, acetylsulphonates, imidazoline derivatives, methyl taurates, sarcosinates, fatty acid amide ether sulfates, alkyl amido betaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic acid derivatives or ethoxylated glycerol fatty acid esters can be added. According to the cosmetic composition of the present invention, excipients containing fluorescent substances, bactericides, hydrotropes, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins and plant extracts can be used. The amount of the component added can be selected according to the dosage form or the purpose of use within a range that does not impair the inherent effects of the cosmetic composition. For example, the amount of the component added can be 0.1 to 10% by weight, preferably 0.1 to 6% by weight, relative to the total weight of the composition, but is not limited thereto.

[0033] The present invention relates to a cosmetic composition containing a composite extract extracted from Radix Ophiopogonis, Radix Ginseng and Schisandrae Chinensis using anion water. The extract can achieve skin inflammation improvement effect by inhibiting inflammatory factor expressed due to heat stimulation, namely cyclooxygenase-2 (COX-2), and can achieve excellent wrinkle improvement and elasticity improvement effect by inhibiting matrix metalloproteinase-1 (MMP-1) and restoring the expression of collagen type I, fibrillin-1 and elastin. It can also induce skin moisturizing by enhancing the expression of ceramide synthase-3 (CERS3) or bleomycin hydrolase (BLMH), so as to be used as a cosmetic composition for relieving heat sensation in menopause or heat sensation caused by a large amount of ultraviolet stimulation or skin thermal damage after laser treatment. DETAILED DESCRIPTION

[0034] Next, the preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein, but can be implemented in other forms. These embodiments are only intended to make the contents introduced in this specification more thorough and complete, and to fully convey the ideas of the present invention to relevant practitioners.

[0035] <Example 1> Preparation of puffing and negative ion water extracts of Ophiopogon japonicus, Ginseng and Schisandra chinensis

[0036] Dried Ophiopogon japonicus (tuberous root) and Ginseng (rhizome) purchased from Gyeongdong Market in Seoul were cut into pieces of less than 5 cm and washed thoroughly before being completely dried. Washed Schisandra chinensis (fruit) was completely dried.

[0037] The prepared raw materials of Radix Ophiopogonis, Radix Ginseng and Fructus Schisandrae Chinensis were respectively put into a rotary expander preheated to above 110°C and then heated by a burner. 2 The lid of the expander was opened to expand at a pressure of 140 to 150° C. and a temperature of 140 to 150° C. 50 g of Ophiopogon japonicus, 25 g of Ginseng and 25 g of Schisandra chinensis that had been expanded were mixed and extracted with 1000 g (w / w) of negative ion water at 50° C. for 4 hours and then filtered. At this time, negative ion water with a pH of 4.0 obtained by an ion purifier equipped with tourmaline was used as negative ion water.

[0038] After the filtrate obtained by the extraction was concentrated using a rotary decompressor, an extract powder was obtained by vacuum drying.

[0039] <Comparative Example 1>

[0040] The extracts of Radix Ophiopogonis, Radix Ginseng and Fructus Schisandrae Chinensis were prepared in the same manner as in Example 1, wherein purified water was used instead of negative ion water to obtain the extracts.

[0041] <Comparative Example 2>

[0042] In the method of Example 1, the step of puffing Ophiopogon japonicus, ginseng and Schisandra chinensis was omitted, and negative ion water was directly added to Ophiopogon japonicus, ginseng and Schisandra chinensis in a dry state to obtain an extract.

[0043] <Comparative Example 3>

[0044] In the method of Example 1, the preservatives in the puffing step of Radix Ophiopogonis, Radix Ginseng and Schisandrae Chinensis were omitted and purified water was directly added to the dried Radix Ophiopogonis, Radix Ginseng and Schisandrae Chinensis instead of negative ion water to produce the extract.

[0045] <Examples 2 to 5, Comparative Examples 4 to 12>

[0046] For Examples 2 to 5 and Comparative Examples 4 to 12, extracts were produced under the conditions shown in Table 1 below.

[0047]

Table 1

[0048]

[0049]

[0050] <Test Example 1> Evaluation of cytotoxicity (Cell cytotoxicity: human immortalized keratinocytes, HaCaT)

[0051] Regarding cytotoxicity evaluation, all extracts were confirmed at different concentrations in human immortalized keratinocytes (HaCaT) and fibroblasts (Fibroblast), and the results of Example 1 and Comparative Examples 1 to 3 are representatively shown.

[0052] After the human immortalized keratinocytes (HaCaT) were inoculated into a 24-well plate and cultured for 24 hours, the culture medium of the cultured cells was replaced with a serum-free medium, and then the samples were treated at different concentrations and cultured for another 24 hours. After the cultured cells were replaced with a medium containing a thiazolyl blue (MTT) solution and reacted, the supernatant was removed. Finally, the absorbance was measured after dissolving the thiazolyl blue-formazan (MTT-formazan) crystals using dimethyl sulfoxide (DMSO), and the results are shown in Table 2 below.

[0053]

Table 2

[0054]

[0055]

[0056] Generally speaking, the cytotoxicity of natural extracts is considered to be minimal when the number of cells is maintained at about 70% or more. Therefore, as shown in the results in Table 2, the extracts of Example 1 and Comparative Examples 1 to 3 did not show cytotoxicity to human immortalized keratinocytes (HaCaT) up to a concentration of 500 ppm. It was also confirmed that the same phenomenon was observed in other extracts.

[0057] <Test Example 2> Evaluation of cytotoxicity (Cell cytotoxicity: Fibroblast)

[0058] After fibroblasts were seeded in a 24-well plate and cultured for 24 hours, evaluation was performed under the same conditions as in Test Example 1. The results are shown in Table 3.

[0059]

Table 3

[0060]

[0061]

[0062] As shown in the results in Table 3, the extracts of Example 1 and Comparative Examples 1 to 3 showed no cytotoxicity to fibroblasts up to a concentration of 500 ppm. It was also confirmed that the same phenomenon was observed in other extracts.

[0063] <Test Example 3> Inflammation relief test on thermal stimulation (cyclooxygenase-2 messenger RNA, COX-2 mRNA)

[0064] After human immortalized keratinocytes (HaCaT) were seeded into 6-well plates and cultured for 24 hours, the culture medium of the cultured cells was replaced with a serum-free medium, followed by heat treatment at 37°C or 43°C for 15 minutes. After heat treatment as described above, the medium was replaced with a new serum-free medium, and then each extract sample was treated at different concentrations and cultured for another 24 hours. TM Lysis Reagent (Qiagen) was used to collect the cultured cells and RNA was isolated according to the method of the manufacturer. After the isolated RNA was quantified, 1 μg of RNA was used to synthesize complementary deoxyribonucleic acid (cDNA) and perform real-time polymerase chain reaction (Rea11-time PCR). The cyclooxygenase-2 (COX-2) and β-actin primers used in the polymerase chain reaction (PCR) were synthesized by Cosmo Genetech (Korea) and used. In the following experiments, all primers were synthesized by Cosmo Genetech (Korea), and the primers used in the present invention are shown in Table 4 below.

[0065]

Table 4

[0066]

[0067]

[0068]

Table 5

[0069]

[0070] The test results, as shown in Table 5, showed that when the extracts of Examples 1 to 7 were used for treatment, the expression of skin inflammation-related factors increased due to thermal stimulation decreased to the level of the control group (Control).

[0071] <Test Example 4> Anti-wrinkle test on thermal stimulation (matrix metalloproteinase-1 messenger RNA, MMP-1 mRNA)

[0072] Each cell was treated with the extract and complementary deoxyribonucleic acid (cDNA) was synthesized in the same manner as in Test Example 3. At this time, fibroblasts (Fibroblast) were used instead of human immortalized human keratinocytes (HaCaT).

[0073] After the synthesis of complementary deoxyribonucleic acid (cDNA), real-time polymerase chain reaction (Real-time PCR) was performed on matrix metalloproteinase-1 (MMP-1). The results are shown in Table 6.

[0074]

Table 6

[0075]

[0076]

[0077] The test results, as shown in Table 6, confirmed that the extracts of Examples 1 to 7 can very effectively inhibit the gene expression of matrix metalloproteinase-1 (MMP-1), a factor associated with skin wrinkles caused by thermal stimulation.

[0078] <Test Example 5> Test I for improving skin elasticity against thermal stimulation

[0079] The extracts were used to treat each cell (fibroblast) and synthesize complementary deoxyribonucleic acid (cDNA) in the same manner as in Test Example 4, and the gene expression of collagen type I, fibrillin-1, and elastin was confirmed by performing real-time polymerase chain reaction (Real-time PCR). The results of Test Example 5-1 are shown in Table 7 (Collagen Type I), the results of Test Example 5-2 are shown in Table 8 (fibrillin-1), and the results of Test Example 5-3 are shown in Table 9 (elastin).

[0080] Test Example 5-1. Collagen Type 1 mRNA

[0081]

Table 7

[0082]

[0083] Test Example 5-2. Fibrin-1 messenger RNA (Fibr i1 1in-1 mRNA)

[0084]

Table 8

[0085]

[0086]

[0087] Test Example 5-3. Elastin messenger RNA (Elastin mRNA)

[0088]

Table 9

[0089]

[0090]

[0091] Referring to the results in Tables 7 to 9, the expressions of skin elasticity factors such as collagen type I, fibrillin-1 and elastin, which were inhibited by thermal stimulation, were restored in the extract treatment groups of Examples 1 to 7.

[0092] <Test Example 6> Moisturizing Efficacy Test - Confirming the Protein Expression of Ceramide Synthase (CERS3, Ceramide synthase-3) and Bleomycin Hydrolase (BLMH, Bleomycin hydrolase)

[0093] Next, the moisturizing ability of the extract directly induced was confirmed.

[0094] To this end, human keratinocytes were seeded in a 60 mm dish and cultured for 24 hours, then replaced with a serum-free medium and cultured after treating the sample at different concentrations. The cells were scraped off with a scraper and pelleted by centrifugation.

[0095] Next, a protein extraction solution was added and incubated, and Qubit TM The protein was quantified using a fluorometer. After adding loading buffer to the quantified protein, it was loaded into SDS-PAGE. After transfer using the iBolt Dry Blotting System, blocking was performed.

[0096] Next, the primary antibodies (bleomycin hydrolase (BLMH), ceramide synthase (CERS3) and 3-glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and secondary antibodies (horseradish peroxidase-labeled goat anti-mouse (GoatAnti-Mouse-HRP), horseradish peroxidase-labeled goat anti-rabbit (Goat Anti-Rabbit-HRP)) were used for processing. After the antibody treatment, the west save gold ECL solution was used for processing, and then the bands were photographed and digitized using chemi doc LuminoGraph. The results are shown in Tables 10 and 11 below.

[0097]

Table 10

[0098]

[0099]

[0100]

Table 11

[0101]

[0102]

[0103] Referring to the results in Table 10 and Table 11, it can be confirmed that the extracts of Examples 1 to 7 of the present invention can significantly increase the expression of genes related to moisture retention.

[0104] <Cosmetic Preparation Example 1> Production of Softening Lotion

[0105] A skin softening lotion (skin care lotion, 100 g) containing the extract of Example 1 as a sample was prepared by a general method and was prepared according to the composition shown in Table 12.

[0106]

Table 12

[0107]

[0108]

[0109] <Cosmetic Preparation Example 2> Production of Nutritional Lotion

[0110] A nutritional lotion (skin care lotion, 100 g) containing the extract of Example 1 was prepared according to the composition shown in Table 13 by a general method.

[0111]

Table 13

[0112] raw material Content (g) Example 1 Extract 3.0 Sitosterol 1.7 Polyglyceryl-2 Oleate 1.5 Cetearyl alcohol polyether 1.2 cholesterol 1.5 Dicetyl Phosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 10.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.3 preservative Trace spices Trace Purified water margin

[0113] <Cosmetic Preparation Example 3> Production of Nutritional Cream

[0114] A nourishing cream (100 g) containing the extract of Example 1 was produced according to the composition shown in Table 14 below by a general method.

[0115]

Table 14

[0116] raw material Content (g) Example 1 Extract 3.0 Sitosterol 4.0 Polyglyceryl-2 Oleate 3.0 Ceramide 0.7 Ceteareth-4 2.0 cholesterol 3.0 Dicetyl Phosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 preservative Trace spices Trace Purified water margin

[0117] <Cosmetic Preparation Example 4> Production of Essence

[0118] According to the composition shown in Table 15, an essence (100 g) containing the extract of Example 1 was prepared by a general method.

[0119]

Table 15

[0120]

[0121]

[0122] <Cosmetic Preparation Example 5> Production of Foundation Cream

[0123] A foundation cream (100 g) containing the extract of Example 1 was produced according to the composition shown in Table 16 below by a general method.

[0124]

Table 16

[0125] raw material Content (g) Example 1 Extract 3.0 beeswax 2.0 Cyclomethicone 2.0 Liquid paraffin 5.0 Squalane 5.0 Stearic acid 2.0 Lipophilic monostearate glyceryl 3.0 Caprylic / capric triglyceride 4.0 glycerin 4.0 Propylene glycol 3.0 Butanediol 3.0 Triethanolamine 1.0 Magnesium aluminum silicate 0.5 pigment 12.0 preservative Trace spices Trace Purified water margin

[0126] <Cosmetic Preparation Example 6> Production of Shampoo

[0127] According to the composition shown in Table 17, a shampoo (100 g) containing the extract of Example 1 was produced by a general method.

[0128]

Table 17

[0129]

[0130]

Claims

1. A cosmetic composition for alleviating thermal stimulation, characterized in that: Contains a composite extract of Ophiopogon japonicus, Ginseng and Schisandra chinensis puff extracted using negative ion water.

2. The cosmetic composition for alleviating heat stimulation according to claim 1, characterized in that: The extract has an improving effect on skin inflammation aggravated by thermal stimulation.

3. The cosmetic composition for alleviating heat stimulation according to claim 2, characterized in that: The extract inhibits the expression of cyclooxygenase-2 (COX-2).

4. The cosmetic composition for alleviating heat stimulation according to claim 1, characterized in that: The extract has an improving effect on skin wrinkles aggravated by thermal stimulation.

5. The cosmetic composition for alleviating heat stimulation according to claim 4, characterized in that: The extract inhibits the expression of matrix metalloproteinase-1 (MMP-1).

6. The cosmetic composition for alleviating heat stimulation according to claim 1, characterized in that: The extract has an enhancing effect on skin elasticity reduced due to thermal stimulation.

7. The cosmetic composition for alleviating heat stimulation according to claim 6, characterized in that: The extract has the effect of restoring the expression of collagen type I, fibrillin-1 or elastin.

8. The cosmetic composition for alleviating heat stimulation according to claim 1, characterized in that: The extract has skin moisturizing effects.

9. The cosmetic composition for alleviating heat stimulation according to claim 8, characterized in that: The extract has the effect of enhancing the protein expression of ceramide synthase (CERS3, Ceramide synthase-3) or bleomycin hydrolase (BLMH, Bleomycin hydrolase).

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