Spirulina fermented fertilizer as well as preparation method and application thereof

Abstract

The invention discloses a spirulina fermented fertilizer as well as a preparation method and application thereof, and the preparation method of the spirulina fermented fertilizer comprises the following steps: uniformly mixing fresh spirulina and sugar to obtain a mixture; mixing the mixture, water, a JM1 strain enlarged culture and a BZ1 strain enlarged culture, and performing fermentation treatment to obtain a fermentation mixture; and (c) adding trichoderma koningii fermentation liquor and trichoderma hamatum fermentation liquor into the fermentation mixture, and mixing to obtain the spirulina fermented fertilizer, according to the invention, the JM1 and BZ1 strains are combined to ferment the spirulina, and then trichoderma koningii and trichoderma hamatum are mixed to adjust the fermentation liquor, so that the prepared spirulina can effectively promote the growth of crops such as Chinese cabbage, pepper and corn, reduce diseases and insect pests and improve the yield of the crops; meanwhile, soil hardening can be relieved, soil loosening can be achieved, use of chemical fertilizers and pesticides is reduced, and the method has important significance on sustainable development of green ecological agriculture.

Description

Technical Field

[0001] The invention relates to the technical field of spirulina fermented fertilizer, and in particular to a spirulina fermented fertilizer and a preparation method and application thereof. Background Art

[0002] Microalgae are a type of eukaryotic or prokaryotic microorganisms with tiny individuals and simple structures that use CO 2 Converted into oil, carbohydrates and proteins. Microalgae grow fast, fix carbon at a high rate, and their carbon fixation efficiency is 10 to 50 times that of general terrestrial plants. In addition to carbon fixation, microalgae can also fix nitrogen and secrete a variety of biologically active substances, such as carotenoids, proteins, fatty acids, plant hormones, extracellular polysaccharides, vitamins and antibiotics, etc., which play an important role in improving soil structure and physical and chemical properties, accumulating soil organic matter, and regulating soil microbial communities. The biologically active substances such as auxin and gibberellin secreted by microalgae have an important role in promoting plant development, seed germination, seedling growth, fruit quality and crop yield. Therefore, as a biological fertilizer, microalgae has the characteristics of trace efficiency and no harm to the environment, and is the best substitute for chemical fertilizers. In addition, the use of mixed fermentation of microalgae and microorganisms to prepare microbial fertilizers with dual algae and fungi is one of the research hotspots in the field of microbial fertilizer development.

[0004] The application of microalgae biofertilizer has become a new growth point for green, efficient, safe and sustainable development of modern agriculture. Spirulina, an economic microalgae of the genus Spirulina in the family Oscillatoriaceae, is an ancient lower aquatic prokaryotic plant of the genus Spirulina. It is suitable for high temperature alkaline environment, has a short growth cycle and high photosynthetic efficiency, and has been industrially produced on a large scale around the world. Spirulina is rich in protein, essential fatty acids, β-carotene, chlorophyll, vitamins, spirulina polysaccharides and minerals, etc. It has multiple functions such as antioxidant, anti-inflammatory, promoting skin wound repair, anti-tumor, lowering blood sugar, lowering blood pressure and weight loss. In the early stage of base breeding, the company found that the waste water after spirulina breeding and the fermented spirulina liquid both promoted the growth of vegetables, and the soil after spraying was looser. On this basis, after multiple methods were tried and repeated experiments, it was verified that the biofertilizer fermented by spirulina can significantly promote the growth of crops, which is of great significance to improving soil fertility, increasing crop yield and quality, and realizing the sustainable development of green ecological agriculture. Summary of the invention

[0005] The purpose of the present invention is to provide a spirulina fermented fertilizer and a preparation method and application thereof. The spirulina fermented fertilizer of the present invention can effectively promote the growth of crops such as cabbage, pepper, corn, etc., reduce pests and diseases, and increase crop yields; at the same time, it can alleviate soil compaction and loosen the soil.

[0006] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0007] A first aspect of the present invention provides a method for preparing a spirulina fermented fertilizer, the method comprising the following steps:

[0008] (a) mixing fresh spirulina and sugar to obtain a mixture;

[0009] (b) mixing the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain and then fermenting them to obtain a fermentation mixture, wherein the JM1 strain is Candida ethanolica, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024847 and a deposit date of April 30, 2024; the BZ1 strain is Brettanomyces custersianus, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M2024846 and a deposit date of April 30, 2024;

[0010] (c) adding Conothellae trifoliata fermentation liquid and hooked trifoliata fermentation liquid into the fermentation mixture and mixing them to obtain the spirulina fermentation fertilizer.

[0011] Preferably, the present invention does not specifically limit the cultivation method of Spirulina, and those skilled in the art may adopt conventional cultivation methods in the art, such as traditional raceway cultivation under a transparent plastic greenhouse.

[0012] Preferably, the culture medium components used in Spirulina cultivation include: NaHCO 3 15.0g / L, Na 2 CO 3 5.00g / L, K 2 HPO 4 0.50g / L, NaNO 3 2.50g / L, K 2 SO 4 1.00g / L, NaCl 1.00g / L, MgSO4·7H 2 O 0.20g / L, CaCl 2 ·2H 2 O 0.20g / L, FeSO 4 7H 2 O 0.01g / L; the water source can be tap water or mountain spring water around the breeding base.

[0013] Preferably, the fresh spirulina is rinsed 2 to 3 times after being harvested and the water is drained.

[0014] Preferably, in step (a), the mass ratio of spirulina to sugar is 1:(0.8-1.2).

[0015] Preferably, the sugar is brown sugar.

[0016] Preferably, in step (b), the mass ratio of the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain is 10:(40-50):(1.8-2.2):(0.8-1.2).

[0017] Preferably, in step (b), the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain are prepared by the following methods respectively:

[0018] The peeled and diced potatoes are mixed with water at a mass ratio of 1:(4-6), steamed, filtered, and the filtrate is collected. The filtrate is then mixed with sugar at a mass ratio of (4-6):1, sterilized, and then inoculated with the JM1 strain or the BZ1 strain and cultured to obtain a JM1 strain expanded culture or a BZ1 strain expanded culture.

[0019] Preferably, the OD600 of the expanded culture of the JM1 strain is 0.8 to 1, and the culture is carried out on a constant temperature shaker at 25° C. and 120 rpm for 70 to 75 hours;

[0020] The OD600 of the expanded culture of the BZ1 strain is 0.6-0.8, and the culture is carried out on a constant temperature shaker at 25° C. and 120 rpm for 90-100 hours.

[0021] Preferably, in step (b), the fermentation treatment is sealed barrel fermentation, the fermentation temperature is 15-25° C., the fermentation time is 4-6 weeks, and the lid is opened once every 3-5 days in the 2nd to 3rd week of fermentation, and then once every 5-7 days.

[0022] Preferably, in the step (c), the volume ratio of the fermentation mixture, the fermentation broth of the koning's trichoderma enzyme and the fermentation broth of the hooked trichoderma enzyme is (8-12):1:1.

[0023] Preferably, in the step (c), the fermentation broth of the koningya enzyme and the fermentation broth of the hooked broth are prepared by the following method:

[0024] After the Trichoderma koningii or the hooked Trichoderma grows to full plate on the PDA culture medium plate, 10 mL of distilled water is added dropwise to the culture plate, the hyphae are scraped off with an inoculation spatula, mixed with the distilled water and broken into a bacterial suspension, the Trichoderma koningii suspension and the hooked Trichoderma suspension are inoculated into the PDB culture medium, respectively, and the Trichoderma koningii suspension or the hooked Trichoderma suspension is diluted and adjusted to OD600 of 0.15-0.18; then diluted 50 times with the PDB culture medium, and then cultured in a constant temperature shaker at 20-25° C. and 100-200 rpm for 100-150 hours, filtered with two layers of gauze, and the filtrate is collected to obtain the Trichoderma koningii enzyme fermentation liquid or the hooked Trichoderma enzyme fermentation liquid.

[0025] The second aspect of the present invention provides a spirulina fermented fertilizer prepared by the above preparation method.

[0026] The third aspect of the present invention provides an application of the spirulina fermented fertilizer prepared by the above preparation method in crop cultivation.

[0027] Preferably, the crops include cabbage, pepper and corn.

[0028] The fourth aspect of the present invention also provides a method for cultivating crops, which comprises spraying the spirulina fermented fertilizer on the surface of the crops.

[0029] Compared with the prior art, the beneficial effects of the present invention include at least:

[0030] The invention ferments spirulina by combining JM1 and BZ1 strains, and then mixes Trichoderma koningii and Trichoderma hooked to adjust the fermentation liquid, so that the prepared spirulina fermentation liquid can effectively promote the growth of crops such as cabbage, pepper, corn, etc., reduce pests and diseases, and increase the yield of crops; at the same time, it can also alleviate soil compaction, loosen the soil, and reduce the use of chemical fertilizers and pesticides, which is of great significance to achieving sustainable development of green ecological agriculture; in addition, the spirulina fertilizer of the invention is simple and easy to operate, low in cost, and suitable for large-scale promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the specific embodiments or the description of the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0032] Figure 1 The growth of the Chinese cabbage seedlings in Experimental Example 2 of the present invention;

[0033] Figure 2 The growth conditions of Chinese cabbage in the field in Experimental Example 3 of the present invention;

[0034] Figure 3 This is the growth condition of the Chinese cabbage at the harvest stage in Experimental Example 3 of the present invention. DETAILED DESCRIPTION

[0035] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the embodiments. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0036] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0037] The raw materials used in the following examples are as follows:

[0038] The spirulina is cultivated in a traditional raceway style under a transparent plastic greenhouse. The spirulina cultivation matrix comprises NaHCO 3 15.0g / L, Na 2 CO 3 5.00g / L, K 2 HPO 4 0.50g / L, NaNO 3 2.50g / L, K 2 SO 4 1.00g / L, NaCl1.00g / L, MgSO 4 7H 2 O 0.20g / L, CaCl 2 ·2H 2 O 0.20g / L, FeSO 4 7H 2 O 0.01g / L; The water source of the aquaculture substrate is the mountain spring water around the aquaculture base.

[0039] The JM1 strain expanded culture and the BZ1 strain expanded culture were prepared by the following methods:

[0040] The peeled and diced potatoes were mixed with water at a mass ratio of 1:5, steamed for 20 minutes, filtered, and the filtrate was collected. The filtrate was then mixed with brown sugar at a mass ratio of 5:1, sterilized, and then inoculated with the JM1 strain or the BZ1 strain and cultured to obtain a JM1 strain expanded culture or a BZ1 strain expanded culture, wherein the OD600 of the JM1 strain expanded culture was 0.9, and the culture was cultured on a constant temperature shaker at 25°C and 120rpm for 72 hours; the OD600 of the BZ1 strain expanded culture was 0.7, and the culture was cultured on a constant temperature shaker at 25°C and 120rpm for 96 hours.

[0041] Kang's trichoderma fermentation broth and hooked trichoderma fermentation broth are prepared by the following method:

[0042] After the Trichoderma koningii or the hooked Trichoderma grows to full plate on the PDA culture medium plate, 10 mL of distilled water is added dropwise to the culture plate, the hyphae are scraped off with an inoculation spatula, mixed with distilled water and broken into a bacterial suspension, and then the Trichoderma koningii suspension and the hooked Trichoderma suspension are inoculated into the PDB culture medium, respectively, to obtain a Trichoderma koningii suspension or the hooked Trichoderma suspension with an OD600 of 0.16; then the suspension is diluted 50 times with the PDB culture medium, and then cultured in a constant temperature shaker at 22° C. and 150 rpm for 120 h, filtered through 2 layers of gauze, and the filtrate is collected to obtain the Trichoderma koningii enzyme fermentation liquid or the hooked Trichoderma enzyme fermentation liquid.

[0043] Trichoderma koningii: purchased from Shanghai Yanzun Biotechnology Co., Ltd., product number YZ-J8138;

[0044] Trichoderma hooked: purchased from Shanghai Xuanke Biotechnology Co., Ltd., product number XK-SH-10237.

[0045] Example 1

[0046] This embodiment is a method for preparing a spirulina fermented fertilizer, and the method for preparing the spirulina fermented fertilizer comprises the following steps:

[0047] (a) washing and draining fresh spirulina, mixing spirulina and brown sugar in a mass ratio of spirulina to sugar of 1:0.8, and standing for 2 hours to obtain a mixture;

[0048] (b) according to the mass ratio of the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain being 10:40:1.8:1.2, the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain are mixed and then sealed and fermented in barrels at a fermentation temperature of 15-25° C. for 4 weeks, the cover is opened once every 3 days in the second week of fermentation, and then the cover is opened once every 5 days to obtain a fermentation mixture, wherein the JM1 strain is Candida ethanolica, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024847 and a deposit date of April 30, 2024; the BZ1 strain is Brettanomyces custersianus, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024846 and a deposit date of April 30, 2024;

[0049] (c) adding the fermentation liquid of Conosula oxyphylla and the fermentation liquid of Hook-shaped Trichoderma into the fermentation mixture in a volume ratio of 8:1:1 to obtain the spirulina fermentation fertilizer.

[0050] Example 2

[0051] This embodiment is a method for preparing a spirulina fermented fertilizer, and the method for preparing the spirulina fermented fertilizer comprises the following steps:

[0052] (a) washing and draining fresh spirulina, mixing spirulina and brown sugar in a mass ratio of spirulina to sugar of 1:1.2, and letting stand for 2 hours to obtain a mixture;

[0053] (b) according to the mass ratio of the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain being 10:50:2.2:0.8, the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain are mixed and then fermented in barrels at a sealed temperature of 15-25° C. for 6 weeks, the cover is opened once every 5 days in the third week of fermentation, and then the cover is opened once every 7 days to obtain a fermentation mixture, wherein the JM1 strain is Candida ethanolica, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024847 and a deposit date of April 30, 2024; the BZ1 strain is Brettanomyces custersianus, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024846 and a deposit date of April 30, 2024;

[0054] (c) adding the fermentation liquid of Conosula oxyphylla and the fermentation liquid of Hook-shaped Trichoderma into the fermentation mixture in a volume ratio of 12:1:1 to obtain the spirulina fermentation fertilizer.

[0055] Example 3

[0056] This embodiment is a method for preparing a spirulina fermented fertilizer, and the method for preparing the spirulina fermented fertilizer comprises the following steps:

[0057] (a) washing and draining fresh spirulina, mixing spirulina and brown sugar in a mass ratio of spirulina to sugar of 1:1, and standing for 2 hours to obtain a mixture;

[0058] (b) according to the mass ratio of the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain being 10:45:2:1, the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain are mixed and then sealed and fermented in barrels at a fermentation temperature of 15-25° C. for 5 weeks, the cover is opened once every 4 days in the second week of fermentation, and then the cover is opened once every 6 days to obtain a fermentation mixture, wherein the JM1 strain is Candida ethanolica, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024847 and a deposit date of April 30, 2024; the BZ1 strain is Brettanomyces custersianus, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024846 and a deposit date of April 30, 2024;

[0059] (c) adding the fermentation liquid of Conosula oxyphylla and the fermentation liquid of Hook-shaped Trichoderma into the fermentation mixture in a volume ratio of 10:1:1 to obtain the spirulina fermentation fertilizer.

[0060] Comparative Example 1

[0061] This comparative example is a method for preparing a spirulina fermented fertilizer, and the method for preparing the spirulina fermented fertilizer comprises the following steps:

[0062] (a) washing and draining fresh spirulina, mixing spirulina and brown sugar in a mass ratio of spirulina to sugar of 1:1, and standing for 2 hours to obtain a mixture;

[0063] (b) according to the mass ratio of the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain being 10:45:2:1, the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain are mixed and then sealed and fermented in barrels, the fermentation temperature is 15-25° C., the fermentation time is 5 weeks, the lid is opened once at an interval of 4 days in the second week of fermentation, and the lid is opened once at an interval of 6 days thereafter to obtain a fermentation mixture, that is, the spirulina fermented fertilizer, wherein the JM1 strain is Candida ethanolica, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024847 and a deposit date of April 30, 2024; the BZ1 strain is Brettanomyces custersianus, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M2024846 and a deposit date of April 30, 2024.

[0064] Experimental Example 1

[0065] This experimental example is a potted test of Chinese cabbage germination using the spirulina fermented fertilizer of Example 3:

[0066] Ordinary flower pots were filled with nutrient soil to sow Chinese cabbage seeds. As for cultivation and observation in a greenhouse, a blank control group (CK) without any fertilizer and only watering, a spirulina fermented fertilizer treatment group (A, i.e., the spirulina fermented liquor of Comparative Example 1) without adding Trichoderma koningii and Trichoderma hooked fermented liquor, and a spirulina fermented fertilizer treatment group (B, i.e., the spirulina fermented liquor of Example 3) with added Trichoderma koningii and Trichoderma hooked fermented liquor were set to observe the germination of the three types of Chinese cabbage seeds and the growth of Chinese cabbage seedlings; the amount of fermented fertilizer added in Group A and Group B was the same, and the application method was to apply 50 mL of original fertilizer to each pot after sowing, dilute it 10 times, and then water it thoroughly, and after observing the germination and breaking of the Chinese cabbage seeds, the same amount of fertilizer was applied to each pot again; the three groups were watered according to the soil dryness and humidity to maintain soil moisture during the growth management period.

[0067] The study found that among the three groups, the growth of pakchoi in group CK was the worst, with short seedlings and easy to be infested with insects; the growth of groups A and B was good, with low disease rates, but group B had the best growth, with pakchoi seedlings being strong and not easy to be infested with insects. That is, spirulina fermented liquid has a growth-promoting effect on pakchoi, and the spirulina fermented liquid with Trichoderma fermented liquid has a better growth-promoting effect on pakchoi and reduces diseases and insect pests.

[0068] Experimental Example 2

[0069] This experimental example is a field test of the spirulina fermented fertilizer of Example 3:

[0070] The land was plowed, farmyard manure was applied as base fertilizer, and then cabbage was sown. Two groups of test fields were set up, one group (A) planted cabbage without fertilizer, and one group (B) planted cabbage with the spirulina fermented fertilizer of Example 3. The growth of the cabbage seedlings was observed 15 days after sowing; the application amount of the spirulina fermented fertilizer of Example 3 was 0.8L per square meter, diluted 10 times, and the fertilization method was watering once every 5 days;

[0071] 15 days after the cabbage was sown, the average height of the cabbage seedlings was measured and counted by the 5-point random sampling method; 10 seedlings were taken from each point, and the average weight of the 10 seedlings was calculated. The results are shown in Table 1; the growth of the cabbage seedlings is shown in Figure 1 As shown;

[0072] Table 1 Cabbage seedling growth detection index

[0073] Group Seedling height (cm) Seedling weight (g) A 8.31±1.75 95.33±11.86 B 12.64±1.37 164.50±18.43

[0074] From Table 1 and Figure 1 It can be seen that the use of spirulina fermented fertilizer in cabbage sowing has better growth, higher seedling height and higher yield.

[0075] Experimental Example 3

[0076] This experimental example is a Chinese cabbage cultivation test of the spirulina fermented fertilizer of Example 3:

[0077] The land was plowed, farmyard manure was applied as base fertilizer, and then cabbage seedlings were transplanted. Two groups of test fields were set up, one group (A) planted cabbage without fertilizer, and one group (B) planted cabbage with the spirulina fermented fertilizer of Example 3, and the growth of the cabbage was observed after planting for 50 days; the fertilization method of the spirulina fermented fertilizer of Example 3 was to apply 4L of diluted fertilizer solution every 7 days per square meter of land, and the diluted fertilizer solution was 1.3L of original fertilizer diluted 6 times;

[0078] The five-point random sampling method was used to measure the average plant height of cabbage and calculate the average fresh weight of cabbage. The results are shown in Table 2. The growth of cabbage in the field is as follows: Figure 2 , Figure 3 As shown;

[0079] Table 2 Cabbage production detection indicators

[0080] Group Seedling height (cm) Seedling weight (kg) A 31.4±4.3 1.3±0.3 B 42.7±3.9 2.1±0.4

[0081] From Table 2 and Figures 2-3 It can be seen that:

[0082] The cabbages grown in group B using spirulina fermented fertilizer generally grew taller than those in group A; Figure 3 The three detailed pictures more clearly confirmed that the cabbages in Group B not only grew tall but also had fewer insect holes, that is, spirulina fertilizer promoted the growth of cabbage and enhanced its resistance to stress.

[0083] Experimental Example 4

[0084] This experimental example is a pepper cultivation test of the spirulina fermented fertilizer of Example 3:

[0085] The land was plowed, farmyard manure was applied as base fertilizer, and peppers were planted respectively. Two groups of test fields were set up, one group (A) did not use fertilizer, and the other group (B) used the spirulina fermented fertilizer of Example 3; the fertilization method of the spirulina fermented fertilizer of Example 3 was to apply 1.0 L of diluted fertilizer solution to each pepper every 7 days, and the diluted fertilizer solution was 100 mL of original fertilizer diluted 10 times;

[0086] After planting, when the peppers were harvested, the 5-point random sampling method was used to measure and count the fresh weight yield of the peppers. The results are shown in Figure 3;

[0087] Table 3 Pepper production statistics

[0088] Group <![CDATA[Output (kg / m 2 )]]> A 4.7±1.1 B 6.5±0.8

[0089] From Table 3, we can see that:

[0090] The fresh weight yield of peppers in group B cultivated with spirulina fermented fertilizer was higher.

[0091] Experimental Example 5

[0092] This experimental example is a corn planting test of the spirulina fermented fertilizer of Example 3:

[0093] The land was plowed, farmyard manure was applied as base fertilizer, and corns were planted respectively. Two groups of test fields were set up, one group (A) did not use fertilizer, and the other group (B) used the spirulina fermented fertilizer of Example 3. The fertilization method of the spirulina fermented fertilizer of Example 3 was to apply 1.0 L of diluted fertilizer solution to each corn every 7 days, and the diluted fertilizer solution was 100 mL of original fertilizer diluted 8 times;

[0094] After planting, when corn was harvested, the 5-point random sampling method was used to measure and count corn yields. The results are shown in Table 4.

[0095] Table 4 Corn production statistics

[0096] Group <![CDATA[Output (kg / m 2 )]]> A 7.2±1.0 B 8.8±1.3

[0097] From Table 4, we can see that:

[0098] The corn yield of Group B cultivated with Spirulina fermented fertilizer was higher.

[0099] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. A method for preparing a spirulina fermented fertilizer, characterized in that: The preparation method of the spirulina fermented fertilizer comprises the following steps: (a) mixing fresh spirulina and sugar to obtain a mixture; (b) mixing the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain and then fermenting them to obtain a fermentation mixture, wherein the JM1 strain is Candida ethanolica, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M 2024847 and a deposit date of April 30, 2024; the BZ1 strain is Brettanomyces custersianus, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC M2024846 and a deposit date of April 30, 2024; (c) adding Conothellae trifoliata fermentation liquid and hooked trifoliata fermentation liquid into the fermentation mixture and mixing, thereby obtaining the spirulina fermentation fertilizer.

2. The preparation method of spirulina fermented fertilizer according to claim 1, characterized in that, In the step (a), the mass ratio of spirulina to sugar is 1:(0.8-1.2).

3. The preparation method of spirulina fermented fertilizer according to claim 1, characterized in that, In the step (b), the mass ratio of the mixture, water, the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain is 10:(40-50):(1.8-2.2):(0.8-1.2).

4. The preparation method of spirulina fermented fertilizer according to claim 1, characterized in that, In the step (b), the expanded culture of the JM1 strain and the expanded culture of the BZ1 strain are respectively prepared by the following methods: The peeled and diced potatoes are mixed with water at a mass ratio of 1:(4-6), steamed, filtered, and the filtrate is collected. The filtrate is then mixed with sugar at a mass ratio of (4-6):1, sterilized, and then inoculated with the JM1 strain or the BZ1 strain and cultured to obtain a JM1 strain expanded culture or a BZ1 strain expanded culture.

5. The method for preparing the spirulina fermented fertilizer according to claim 4, characterized in that: The OD600 of the JM1 strain expansion culture is 0.8-1, and the culture is cultured on a constant temperature shaker at 25°C and 120 rpm for 70-75 hours; The OD600 of the expanded culture of the BZ1 strain is 0.6-0.8, and the culture is carried out on a constant temperature shaker at 25° C. and 120 rpm for 90-100 hours.

6. The method for preparing the spirulina fermented fertilizer according to claim 1, characterized in that: In the step (b), the fermentation treatment is sealed barrel fermentation, the fermentation temperature is 15-25° C., the fermentation time is 4-6 weeks, and the lid is opened and stirred once every 3-5 days in the 2nd to 3rd week of fermentation, and then the lid is opened and stirred once every 5-7 days.

7. The method for preparing the spirulina fermented fertilizer according to claim 1, characterized in that: In the step (c), the volume ratio of the fermentation mixture, the fermentation broth of the koningya enzyme and the fermentation broth of the hooked broth is (8-12):1:

1.

8. The method for preparing the spirulina fermented fertilizer according to claim 1, characterized in that: In the step (c), the fermentation broth of the koningya enzyme and the fermentation broth of the hooked broth are prepared by the following method: After the Trichoderma koningii or the hooked Trichoderma grows to full plate on the PDA culture medium plate, 10 mL of distilled water is added dropwise to the culture plate, the hyphae are scraped off with an inoculation spatula, and the mycelia are mixed with the distilled water to break into a bacterial suspension, the Trichoderma koningii suspension and the hooked Trichoderma suspension are inoculated into the PDB culture medium, respectively, and the concentration of the inoculation liquid is diluted to adjust the concentration of the Trichoderma koningii suspension or the hooked Trichoderma suspension to OD600 of 0.15-0.18; then the suspension is diluted 50 times with the PDB culture medium and shaken for culture under the constant temperature shaking condition of 20-25° C. and 100-200 rpm for 100-150 hours, filtered with two layers of gauze, and the filtrate is collected to obtain the Trichoderma koningii enzyme fermentation liquid or the hooked Trichoderma enzyme fermentation liquid.

9. The spirulina fermented fertilizer prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the spirulina fermented fertilizer prepared by the preparation method according to any one of claims 1 to 8 in crop cultivation.

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