Cell culture device for drug screening

Abstract

The invention discloses a cell culture device for drug screening, and relates to the technical field of cell culture.The cell culture device comprises an outer box body, a culture device body and a base fixedly connected to the bottom of the outer box body, a box door is hinged to the front face of the outer box body and is in an L shape, and the cell culture device further comprises an observation window fixedly installed on one side of the front face of the box door; a uniform shaking mechanism is arranged on the inner bottom surface of the outer box body, and a stirring mechanism is arranged at the upper part in the outer box body; the shaking mechanism comprises a driving motor fixedly mounted on the inner bottom surface of the outer box body, the output end of the driving motor is fixedly connected with a first main belt wheel, and the top of the first main belt wheel is fixedly connected with an L-shaped rod; the stirring mechanism comprises a top plate and a pressing plate, and the top plate and the pressing plate are fixedly mounted, so that the problems that the cells in the culture device cannot be uniformly dispersed well, the uniform mixing effect is relatively general, and the culture effect and the culture quality of the suspension cells are also reduced due to the fact that stirring and uniform mixing are performed only through a single stirring mechanism are solved.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture device for drug screening. Background Art

[0002] Cells have functions such as movement, nutrition and reproduction. When making drugs through cells, the cells need to be cultured in advance. At this time, a culture device is required. When culturing suspended cells, auxiliary stirring and mixing are required.

[0003] For example, a cell culture device for cell drug screening with announcement number CN220643147U includes a support seat, the top of the support seat is respectively fixedly connected with a support rod and a driving motor, the output shaft of the driving motor is fixedly connected with a driving shaft through a coupling, the top of the driving shaft is fixedly connected with a driving wheel, the outer wall of the driving wheel is provided with a transmission belt, the top of the support seat is movably connected with a driven shaft, the top of the driven shaft is fixedly connected with a driven wheel, the top of the driving wheel and the driven wheel are both fixedly connected with a screw rod, and the device is provided with a support seat, a support rod, a driving motor, and an output shaft of the driving motor. The driving motor, driving shaft, driving wheel, transmission belt, driven shaft, driven wheel, screw rod, culture tank, stirring mechanism, fixed plate and discharge pipe are conducive to driving the stirring mechanism to rise and fall, thereby facilitating the maintenance of the stirring mechanism and avoiding the inconvenience of maintenance. However, for suspended cell culture, a suspended cell shaker is also required for auxiliary mixing operation. The device only uses a single stirring mechanism for stirring and mixing, and cannot evenly disperse the cells inside the culture device. The mixing effect is relatively general, which also reduces the culture effect and culture quality of the suspended cells, and has certain defects.

[0004] Therefore, a cell culture device for drug screening is proposed to solve the above-mentioned problems. Summary of the invention

[0005] The purpose of the present invention is to provide a cell culture device for drug screening to solve the problem that stirring and mixing only by a single stirring mechanism cannot evenly disperse the cells inside the culture device, the mixing effect is relatively general, and the culture effect and quality of suspended cells are reduced.

[0006] To achieve the above object, the present invention provides the following technical solution: a cell culture device for drug screening, comprising an outer box, a culture device body and a base fixedly connected to the bottom of the outer box, wherein a door is hingedly connected to the front of the outer box, and the door is L-shaped;

[0007] Also includes:

[0008] An observation window is fixedly installed on one side of the front of the box door, a shaking mechanism is provided on the inner bottom surface of the outer box body, and a stirring mechanism is provided on the upper part of the inner part of the outer box body;

[0009] The shaking mechanism includes a driving motor fixedly mounted on the bottom surface of the outer box body, the output end of the driving motor is fixedly connected to a main pulley 1, the top of the main pulley 1 is fixedly connected to an L-shaped rod, a fixing strip is symmetrically fixedly connected to one side of the inner back side of the outer box body, one end of the fixing strip is fixedly connected to a fixing sleeve, the outer side of the L-shaped rod is sleeved with a limit frame, and sliding strips are symmetrically fixedly mounted on both sides of the limit frame;

[0010] The sliding bar is slidably connected to the fixed sleeve, an auxiliary inclined plate is symmetrically fixedly installed on the inner top surface of the outer box body, the inclined surface of the auxiliary inclined plate abuts against the limit frame, a cross bar is fixedly connected inside the sliding bar, and the two ends of the cross bar are symmetrically fixedly connected to the limit block, the limit block is slidably connected to the fixed sleeve, and the outer side of the cross bar is sleeved with a spring;

[0011] The top of the limit frame is fixedly connected with a top block, the top of the top block is fixedly connected with a placement plate, the top of the placement plate is installed with a clamping block, the inner bottom surface of the outer box body is symmetrically rotated with a vertical rod, the lower outer side of the vertical rod is fixedly connected with a slave pulley 1, and the main pulley 1 is adaptively connected with the slave pulley 1 through a toothed belt;

[0012] The stirring mechanism includes a top plate and a pressure plate, the top plate and the pressure plate are fixedly installed, the bottom of the top plate is symmetrically rotatably connected with side rods on both sides, the side rods are fixed to the vertical rods by bolts, the middle of the side rods is fixedly connected with a second slave pulley, the middle of the bottom of the top plate is rotatably connected with a center rod, the middle of the center rod is fixedly connected with a second main pulley, the second main pulley is adaptably connected to the second slave pulley by a toothed belt, and the lower part of the center rod is fixedly connected with a stirring rod.

[0013] Preferably, the two fixing sleeves are respectively located on both sides of the L-shaped rod, the sliding bar is located in the middle of the outer side of the limiting frame, the sliding bar is fixedly connected to the limiting frame, and two sliding bars are provided.

[0014] By adopting the above technical solution, it is easy to achieve reciprocating shaking and mixing of the culture device body, which improves the shaking effect of the culture device body. In addition, the stirring position change of the stirring rod improves the stirring effect, which facilitates the full mixing of the cells inside the culture device body.

[0015] Preferably, a connecting rod is fixedly connected to the bottom of the auxiliary inclined plate, and the auxiliary inclined plate is fixedly connected to the outer box body through the connecting rod. The two auxiliary inclined plates are respectively located at the front and rear sides of the limit frame, and the inclined surface of the auxiliary inclined plate abuts against the limit frame.

[0016] By adopting the above technical solution, the L-shaped rod rotates to drive the limit frame to move, and the inclined surface of the auxiliary inclined plate abuts against the limit frame, so that the limit frame moves along the inclined surface of the auxiliary inclined plate.

[0017] Preferably, the springs are symmetrically arranged, the springs are located on both sides of the sliding bar, one end of the spring is fixedly connected to the sliding bar, and the other end of the spring is fixedly connected to the limit block.

[0018] By adopting the above technical solution, when the sliding bar moves, the spring will be squeezed or stretched, and the spring assists the sliding bar to move smoothly.

[0019] Preferably, there are no less than two cross bars, the inner side wall of the fixing sleeve is symmetrically provided with outer grooves, the cross bars are slidably connected to the outer grooves, the interior of the fixing sleeve is symmetrically provided with inner grooves, the outer grooves are connected to the inner grooves, and the limit block is slidably connected to the inner grooves.

[0020] By adopting the above technical solution, when the sliding bar drives the cross bar to move, the cross bar slides inside the outer groove, and the cross bar drives the limit block to slide in the inner groove, and the limit block is used to limit the cross bar.

[0021] Preferably, a mounting block is symmetrically fixedly connected to the top of the placement plate, an inner thread of the mounting block is connected to a tightening screw rod, and one end of the tightening screw rod is rotatably connected to the clamping block.

[0022] By adopting the above technical solution, the culture device body is placed on the top of the placement plate, and the tightening screw is screwed, which drives the clamping block to move, so that the clamping blocks on both sides can tighten and fix the culture device body.

[0023] Preferably, a mounting frame plate is fixed symmetrically in the middle of the outer box body, the placement plate is located between the two mounting frame plates, the placement plate is fitted with the mounting frame plates, and the vertical rod is rotatably connected to the mounting frame plates.

[0024] By adopting the above technical solution, the mounting frame plate is used to limit the placement plate and maintain the smooth movement of the placement plate.

[0025] Preferably, a connecting rod is fixedly connected to the bottom of the top plate, the connecting rod is symmetrically arranged, the bottom end of the connecting rod is fixedly connected to the pressing plate, and the center rod is rotatably connected to the pressing plate.

[0026] By adopting the above technical solution, the driving motor drives the main pulley 1 to rotate, the main pulley 1 drives the slave pulley 1 to rotate through the toothed belt, the slave pulley 1 drives the vertical rod and the side rod to rotate, the side rod drives the slave pulley 2 to rotate, the slave pulley 2 drives the main pulley 2 to rotate through the toothed belt, and the main pulley 2 drives the center rod and the stirring rod to rotate.

[0027] Preferably, the bottom surface of the pressing plate is in contact with the top of the main body of the culture device, the stirring rods are evenly arranged at equal distances, there are no less than three stirring rods, and the stirring rods are located inside the main body of the culture device.

[0028] By adopting the above technical solution, the stirring rod stirs and mixes the interior of the culture device body, and the placement plate drives the culture device body to move back and forth obliquely to shake it, so that the position of the stirring rod inside the culture device body changes, thereby improving the stirring effect.

[0029] Compared with the prior art, the present invention has the following beneficial effects: a shaking mechanism and a stirring mechanism are provided, so that the main body of the culture device can be shaken back and forth for mixing, thereby improving the shaking effect on the main body of the culture device, and the stirring position change of the stirring rod can improve the stirring effect, thereby facilitating the full mixing of the cells inside the main body of the culture device;

[0030] 1. A shaking mechanism is provided. By opening the box door, the main body of the culture device is placed on the top of the placement plate, and the tightening screw rod is screwed. The tightening screw rod drives the clamping block to move, so that the clamping blocks on both sides tighten and fix the main body of the culture device. After the stirring mechanism is installed, the box door is closed and the driving motor is started. The driving motor drives the L-shaped rod to rotate. The L-shaped rod is initially located at one end inside the limit frame. Due to the limit of the limit frame, the L-shaped rod rotates to drive the limit frame to move. The inclined surface of the auxiliary inclined plate abuts against the limit frame, so that the limit frame moves along the inclined surface of the auxiliary inclined plate. The limit frame drives the sliding bar to move, and the sliding bar drives the cross bar to move. The cross bar slides inside the outer groove, and the cross bar carries The movable limit block slides in the inner groove, and the limit block is used to limit the cross bar. When the L-shaped rod rotates 90 degrees, the limit frame moves an oblique distance, so that the sliding bar moves obliquely toward the inside of the fixed sleeve on one side; when the L-shaped rod rotates 180 degrees, the limit frame is reset, and the L-shaped rod is located at the other end of the inner part of the limit frame. When the L-shaped rod continues to rotate 180 to 360 degrees, the limit frame will move obliquely toward the fixed sleeve on the other side and reset, so that when the L-shaped rod rotates one circle, the limit frame will move obliquely back and forth, and the limit frame drives the top block and the placement plate to move, thereby driving the main body of the culture device on the top to shake back and forth for mixing, thereby improving the shaking effect of the main body of the culture device;

[0031] 2. A stirring mechanism is provided. When installing, the side rod and the vertical rod are fixed by bolts. The stirring rod is located at the inner center of the culture device body. When the driving motor rotates, the driving motor drives the main pulley 1 to rotate. The main pulley 1 drives the slave pulley 1 to rotate through the toothed belt. The slave pulley 1 drives the vertical rod and the side rod to rotate. The side rod drives the slave pulley 2 to rotate. The slave pulley 2 drives the main pulley 2 to rotate through the toothed belt. The main pulley 2 drives the center rod and the stirring rod to rotate, so that the stirring rod stirs and mixes the inside of the culture device body. The main pulley 1, the slave pulley 1, the slave pulley 2 and the main pulley 2 all adopt synchronous pulleys to ensure transmission stability. The placement plate drives the culture device body to move back and forth obliquely to shake, so that the stirring rod changes its position in the culture device body, improves the stirring effect, and facilitates the full mixing of the cells inside the culture device body. The problem that stirring and mixing only by a single stirring mechanism cannot well disperse the cells inside the culture device, the mixing effect is relatively general, and the culture effect and culture quality of suspended cells are reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a schematic diagram of the internal structure of the outer box of the present invention;

[0033] Figure 2 This is a schematic diagram of the cross-sectional structure of the outer box body of the present invention;

[0034] Figure 3 For the present invention Figure 2 The enlarged structural diagram at A in the middle;

[0035] Figure 4 This is a schematic diagram of the installation structure of the fixing sleeve of the present invention;

[0036] Figure 5 This is a schematic diagram of the cross-sectional structure of the fixing sleeve of the present invention;

[0037] Figure 6 For the present invention Figure 5 The enlarged structural diagram at B in the middle;

[0038] Figure 7 For the present invention Figure 5 The enlarged structural diagram at C in the middle;

[0039] Figure 8 This is a schematic diagram of the oblique movement of the limit frame of the present invention;

[0040] Fig. 9 This is a schematic diagram of the top plate installation structure of the present invention;

[0041] Fig.10 For the present invention Fig. 9 The enlarged structural diagram at D in the middle;

[0042] Fig.11It is a schematic diagram of the three-dimensional overall structure of the present invention.

[0043] In the figure: 1. outer box body; 2. base; 3. box door; 4. observation window; 5. shaking mechanism; 51. driving motor; 52. main pulley 1; 53. L-shaped rod; 54. fixing bar; 55. fixing sleeve; 56. limit frame; 57. sliding bar; 58. auxiliary inclined plate; 59. cross bar; 510. limit block; 511. spring; 512. outer groove; 513. inner groove; 514. top block; 515. placement plate; 516. mounting block; 517. tightening screw rod; 518. clamping block; 519. vertical rod; 520. slave pulley 1; 521. mounting frame plate; 6. stirring mechanism; 61. top plate; 62. connecting rod; 63. pressing plate; 64. side rod; 65. slave pulley 2; 66. center rod; 67. main pulley 2; 68. stirring rod; 7. culture device body. DETAILED DESCRIPTION

[0044] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] See also Figure 1-Figure 11 The present invention provides a technical solution: a cell culture device for drug screening, comprising an outer box body 1, a culture device body 7 and a base 2 fixedly connected to the bottom of the outer box body 1, a door 3 is hinged on the front of the outer box body 1, the door 3 is L-shaped, and an observation window 4 is fixedly installed on one side of the front of the door 3.

[0046] A shaking mechanism 5 is provided on the inner bottom surface of the outer box body 1, and the shaking mechanism 5 includes a driving motor 51 fixedly installed on the inner bottom surface of the outer box body 1, the output end of the driving motor 51 is fixedly connected to a main pulley 52, the top of the main pulley 52 is fixedly connected to an L-shaped rod 53, a fixing bar 54 is symmetrically fixedly connected to one side of the inner back side of the outer box body 1, one end of the fixing bar 54 is fixedly connected to a fixing sleeve 55, the outer side of the L-shaped rod 53 is sleeved with a limit frame 56, and sliding bars 57 are symmetrically fixedly installed on both sides of the limit frame 56.

[0047] The two fixing sleeves 55 are respectively located on both sides of the L-shaped rod 53 . The sliding bar 57 is located in the middle of the outer side of the limiting frame 56 . The sliding bar 57 is fixedly connected to the limiting frame 56 . Two sliding bars 57 are provided.

[0048] The sliding bar 57 is slidably connected to the fixed sleeve 55, and an auxiliary inclined plate 58 is symmetrically fixedly installed on the inner top surface of the outer box body 1. The inclined surface of the auxiliary inclined plate 58 abuts against the limit frame 56. A cross bar 59 is fixedly connected to the inside of the sliding bar 57, and the two ends of the cross bar 59 are symmetrically fixedly connected to the limit blocks 510. The limit blocks 510 are slidably connected to the fixed sleeve 55, and a spring 511 is sleeved on the outer side of the cross bar 59.

[0049] The bottom of the auxiliary inclined plate 58 is fixedly connected with a connecting rod, and the auxiliary inclined plate 58 is fixedly connected to the outer box body 1 through the connecting rod. The two auxiliary inclined plates 58 are respectively located at the front and rear sides of the limit frame 56, and the inclined surface of the auxiliary inclined plate 58 abuts against the limit frame 56.

[0050] The top of the limit frame 56 is fixedly connected to a top block 514, the top of the top block 514 is fixedly connected to a placement plate 515, the top of the placement plate 515 is installed with a clamping block 518, the inner bottom surface of the outer box body 1 is symmetrically connected to a vertical rod 519, the lower outer side of the vertical rod 519 is fixedly connected to a slave pulley 520, and the main pulley 52 and the slave pulley 520 are adaptively connected by a toothed belt.

[0051] The spring 511 is symmetrically arranged. The spring 511 is located on both sides of the sliding bar 57 . One end of the spring 511 is fixedly connected to the sliding bar 57 , and the other end of the spring 511 is fixedly connected to the limit block 510 .

[0052] There are no less than two cross bars 59 , the inner side wall of the fixed sleeve 55 is symmetrically provided with outer grooves 512 , the cross bars 59 are slidably connected to the outer grooves 512 , the interior of the fixed sleeve 55 is symmetrically provided with inner grooves 513 , the outer grooves 512 are connected to the inner grooves 513 , and the limit block 510 is slidably connected to the inner groove 513 .

[0053] A mounting block 516 is symmetrically fixedly connected to the top of the placement plate 515 , and a tightening screw rod 517 is connected to the inner thread of the mounting block 516 , and one end of the tightening screw rod 517 is rotatably connected to the clamping block 518 .

[0054] A mounting frame plate 521 is fixed symmetrically in the middle of the outer box body 1 , the placement plate 515 is located between the two mounting frame plates 521 , the placement plate 515 fits the mounting frame plates 521 , and the vertical rod 519 is rotatably connected to the mounting frame plates 521 .

[0055] Embodiment 1: Figure 1-Figure 8As shown, a shaking mechanism 5 is provided. By opening the box door 3, the culture device body 7 is placed on the top of the placement plate 515, and the tightening screw rod 517 is screwed. The tightening screw rod 517 drives the clamping block 518 to move, so that the clamping blocks 518 on both sides tighten and fix the culture device body 7. After the stirring mechanism 6 is installed, the box door 3 is closed and the drive motor 51 is started to work. The drive motor 51 drives the L-shaped rod 53 to rotate. The L-shaped rod 53 is initially located at one end of the inner side of the limit frame 56. Due to the limit of the limit frame 56, the L-shaped rod 53 rotates to drive the limit frame 56 to rotate. The frame 56 moves, and the inclined surface of the auxiliary inclined plate 58 abuts against the limit frame 56, so that the limit frame 56 moves along the inclined surface of the auxiliary inclined plate 58, and the limit frame 56 drives the sliding bar 57 to move, and the sliding bar 57 drives the cross bar 59 to move, and the cross bar 59 slides inside the outer groove 512, and the cross bar 59 drives the limit block 510 to slide in the inner groove 513, and the limit block 510 is used to limit the cross bar 59. When the L-shaped rod 53 rotates ninety degrees, the limit frame 56 moves an oblique distance, so that the sliding bar 57 moves obliquely toward the inside of the fixed sleeve 55 on one side.

[0056] When the L-shaped rod 53 rotates 180 degrees, the limit frame 56 is reset. At this time, the L-shaped rod 53 is located at the other end inside the limit frame 56. When the L-shaped rod 53 continues to rotate 180 degrees to 360 degrees, the limit frame 56 will move obliquely toward the fixed sleeve 55 on the other side and reset, so that when the L-shaped rod 53 rotates one circle, the limit frame 56 will move back and forth obliquely. The limit frame 56 drives the top block 514 and the placement plate 515 to move, thereby driving the culture device body 7 on the top to shake back and forth for mixing, thereby improving the shaking effect of the culture device body 7.

[0057] A stirring mechanism 6 is arranged on the upper part of the interior of the outer box body 1, and the stirring mechanism 6 includes a top plate 61 and a pressure plate 63. The top plate 61 and the pressure plate 63 are fixedly installed, and the bottom sides of the top plate 61 are symmetrically rotatably connected with side rods 64, and the side rods 64 are fixed to the vertical rod 519 by bolts. The middle of the side rods 64 is fixedly connected to the slave pulley 2 65, and the middle of the bottom of the top plate 61 is rotatably connected with a center rod 66. The middle of the center rod 66 is fixedly connected with a main pulley 2 67, and the main pulley 2 67 is adapted to be connected to the slave pulley 2 65 by a toothed belt. The lower part of the center rod 66 is fixedly connected to a stirring rod 68.

[0058] A connecting rod 62 is fixedly connected to the bottom of the top plate 61, and both sides of the top plate 61 fit into the inner side walls of the outer box body 1 so that the top plate 61 will not move during subsequent work. The connecting rod 62 is symmetrically arranged, and the bottom end of the connecting rod 62 is fixedly connected to the pressure plate 63, and the center rod 66 is rotatably connected to the pressure plate 63.

[0059] The bottom surface of the pressing plate 63 is in contact with the top of the culture device body 7 . The stirring rods 68 are evenly arranged at equal distances. There are no less than three stirring rods 68 . The stirring rods 68 are located inside the culture device body 7 .

[0060] Embodiment 2: Figure 9-11 As shown, a stirring mechanism 6 is provided. During installation, the side rod 64 and the vertical rod 519 are fixed by bolts. The stirring rod 68 is located at the inner center of the culture device body 7. When the driving motor 51 rotates, the driving motor 51 drives the main pulley 1 52 to rotate. The main pulley 1 52 drives the slave pulley 1 520 to rotate through a toothed belt. The slave pulley 1 520 drives the vertical rod 519 and the side rod 64 to rotate. The side rod 64 drives the slave pulley 2 65 to rotate. The slave pulley 2 65 drives the main pulley 2 67 to rotate through a toothed belt. The main pulley 2 67 drives the center rod 66 and the stirring rod 68 to rotate, so that the stirring rod 68 stirs and mixes the inside of the culture device body 7. The main pulley 1 52, the slave pulley 1 520, the slave pulley 2 65 and the main pulley 2 67 all use synchronous pulleys to ensure transmission stability.

[0061] The placement plate 515 will drive the culture device body 7 to move back and forth obliquely and shake, so that the internal position of the stirring rod 68 in the culture device body 7 changes, thereby improving the stirring effect and facilitating the full mixing of the cells inside the culture device body 7. This solves the problem that stirring and mixing only by a single stirring mechanism cannot evenly disperse the cells inside the culture device, the mixing effect is relatively general, and the culture effect and quality of the suspended cells are reduced.

[0062] Working principle: When using the device, first, Figure 1-Figure 11 As shown, by opening the box door 3, the culture device body 7 is placed on the top of the placement plate 515, and the tightening screw 517 is screwed. The tightening screw 517 drives the clamping block 518 to move, so that the clamping blocks 518 on both sides tighten and fix the culture device body 7. During installation, the side rod 64 and the vertical rod 519 are fixed by bolts, and the stirring rod 68 is located at the inner center of the culture device body 7. The box door 3 is closed and the drive motor 51 is started.

[0063] The driving motor 51 drives the L-shaped rod 53 to rotate. The L-shaped rod 53 is initially located at one end inside the limit frame 56. Due to the limitation of the limit frame 56, the L-shaped rod 53 rotates to drive the limit frame 56 to move. The inclined surface of the auxiliary inclined plate 58 abuts against the limit frame 56, so that the limit frame 56 moves along the inclined surface of the auxiliary inclined plate 58. The limit frame 56 drives the sliding bar 57 to move, and the sliding bar 57 drives the cross bar 59 to move. The cross bar 59 slides inside the outer groove 512. The cross bar 59 drives the limit block 510 to slide in the inner groove 513. The limit block 510 is used to limit the cross bar 59. When the L-shaped rod 53 rotates ninety degrees, the limit frame 56 moves an oblique distance, so that the sliding bar 57 moves obliquely toward the inside of the fixed sleeve 55 on one side.

[0064] When the L-shaped rod 53 rotates 180 degrees, the limit frame 56 is reset. At this time, the L-shaped rod 53 is located at the other end inside the limit frame 56. When the L-shaped rod 53 continues to rotate 180 degrees to 360 degrees, the limit frame 56 will move obliquely toward the fixed sleeve 55 on the other side and reset, so that when the L-shaped rod 53 rotates one circle, the limit frame 56 will move back and forth obliquely. The limit frame 56 drives the top block 514 and the placement plate 515 to move, thereby driving the culture device body 7 on the top to shake back and forth for mixing, thereby improving the shaking effect of the culture device body 7.

[0065] While the driving motor 51 is rotating, the driving motor 51 drives the main pulley 52 to rotate, the main pulley 52 drives the slave pulley 520 to rotate through a toothed belt, the slave pulley 520 drives the vertical rod 519 and the side rod 64 to rotate, the side rod 64 drives the slave pulley 65 to rotate, the slave pulley 65 drives the main pulley 67 to rotate through a toothed belt, the main pulley 67 drives the center rod 66 and the stirring rod 68 to rotate, so that the stirring rod 68 stirs and mixes the interior of the culture device main body 7, the main pulley 52, the slave pulley 520, the slave pulley 65 and the main pulley 67 all use synchronous pulleys to ensure transmission stability, and the placement plate 515 will drive the culture device main body 7 to move back and forth obliquely to shake, so that the internal position of the stirring rod 68 in the culture device main body 7 changes, thereby improving the stirring effect.

[0066] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0067] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell culture device for drug screening, comprising an outer box (1), a culture device body (7) and a base (2) fixedly connected to the bottom of the outer box (1), wherein a door (3) is hingedly connected to the front of the outer box (1), and the door (3) is L-shaped; It is characterized in that Also includes: An observation window (4) is fixedly mounted on one side of the front of the box door (3); a shaking mechanism (5) is disposed on the inner bottom surface of the outer box body (1); and a stirring mechanism (6) is disposed on the upper portion of the inner portion of the outer box body (1); The shaking mechanism (5) comprises a driving motor (51) fixedly mounted on the inner bottom surface of the outer box body (1); the output end of the driving motor (51) is fixedly connected to a main pulley (52); the top of the main pulley (52) is fixedly connected to an L-shaped rod (53); a fixing bar (54) is symmetrically fixedly connected to one side of the inner back surface of the outer box body (1); one end of the fixing bar (54) is fixedly connected to a fixing sleeve (55); the outer side of the L-shaped rod (53) is sleeved with a limiting frame (56); sliding bars (57) are symmetrically fixedly mounted on both sides of the limiting frame (56); The sliding bar (57) is slidably connected to the fixed sleeve (55); an auxiliary inclined plate (58) is symmetrically fixedly installed on the inner top surface of the outer box body (1); the inclined surface of the auxiliary inclined plate (58) abuts against the limit frame (56); a cross bar (59) is fixedly connected inside the sliding bar (57); two ends of the cross bar (59) are symmetrically fixedly connected to limit blocks (510); the limit blocks (510) are slidably connected to the fixed sleeve (55); and a spring (511) is sleeved on the outer side of the cross bar (59); The top of the limit frame (56) is fixedly connected to a top block (514), the top of the top block (514) is fixedly connected to a placement plate (515), the top of the placement plate (515) is installed with a clamping block (518), the inner bottom surface of the outer box body (1) is symmetrically rotatably connected to a vertical rod (519), the lower outer side of the vertical rod (519) is fixedly connected to a slave pulley (520), and the main pulley (52) and the slave pulley (520) are adaptively connected via a toothed belt; The stirring mechanism (6) comprises a top plate (61) and a pressure plate (63), wherein the top plate (61) and the pressure plate (63) are fixedly installed, and the bottom sides of the top plate (61) are symmetrically rotatably connected with side rods (64), and the side rods (64) are fixed to the vertical rods (519) by bolts, and the middle of the side rods (64) is fixedly connected to the second slave pulley (65), and the middle of the bottom of the top plate (61) is rotatably connected with a center rod (66), and the middle of the center rod (66) is fixedly connected to the second main pulley (67), and the second main pulley (67) is connected to the second slave pulley (65) by a toothed belt adapter, and the lower part of the center rod (66) is fixedly connected to a stirring rod (68).

2. The cell culture device for drug screening according to claim 1, characterized in that: The two fixing sleeves (55) are respectively located on both sides of the L-shaped rod (53); the sliding bar (57) is located in the middle of the outer side of the limiting frame (56); the sliding bar (57) is fixedly connected to the limiting frame (56); and two sliding bars (57) are provided.

3. The cell culture device for drug screening according to claim 1, characterized in that: The bottom of the auxiliary inclined plate (58) is fixedly connected with a connecting rod, and the auxiliary inclined plate (58) is fixedly connected to the outer box body (1) through the connecting rod. The two auxiliary inclined plates (58) are respectively located on the front and rear sides of the limit frame (56), and the inclined surface of the auxiliary inclined plate (58) is in contact with the limit frame (56).

4. The cell culture device for drug screening according to claim 1, characterized in that: The spring (511) is symmetrically arranged, and the spring (511) is located on both sides of the sliding bar (57). One end of the spring (511) is fixedly connected to the sliding bar (57), and the other end of the spring (511) is fixedly connected to the limit block (510).

5. The cell culture device for drug screening according to claim 1, characterized in that: There are no less than two cross bars (59), the inner side wall of the fixing sleeve (55) is symmetrically provided with outer grooves (512), the cross bars (59) are slidably connected to the outer grooves (512), the interior of the fixing sleeve (55) is symmetrically provided with inner grooves (513), the outer grooves (512) are communicated with the inner grooves (513), and the limit block (510) is slidably connected to the inner grooves (513).

6. The cell culture device for drug screening according to claim 1, characterized in that: A mounting block (516) is symmetrically fixedly connected to the top of the placement plate (515), and a tightening screw rod (517) is internally threadedly connected to the mounting block (516), and one end of the tightening screw rod (517) is rotatably connected to a clamping block (518).

7. The cell culture device for drug screening according to claim 1, characterized in that: A mounting frame plate (521) is fixed symmetrically in the middle of the outer box body (1) in an upper and lower manner, the placement plate (515) is located between the two mounting frame plates (521), the placement plate (515) is in close contact with the mounting frame plates (521), and the vertical rod (519) is rotatably connected to the mounting frame plates (521).

8. The cell culture device for drug screening according to claim 1, characterized in that: The bottom of the top plate (61) is fixedly connected with a connecting rod (62), the connecting rod (62) is symmetrically arranged, the bottom end of the connecting rod (62) is fixedly connected to the pressing plate (63), and the center rod (66) is rotatably connected to the pressing plate (63).

9. The cell culture device for drug screening according to claim 1, characterized in that: The bottom surface of the pressing plate (63) is in contact with the top of the culture device body (7), and the stirring rods (68) are evenly arranged at equal distances. There are no less than three stirring rods (68), and the stirring rods (68) are located inside the culture device body (7).

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