Cell co-culture device and method

Abstract

The invention relates to the technical field of medical experiments, and discloses a cell co-culture device and method.The cell co-culture device comprises a cell co-culture unit which comprises a first culture tube groove and a second culture tube groove, and the first culture tube groove and the second culture tube groove define a tubular structure with the two ends open and the middle provided with a flowing channel; a liquid inlet pipe and a liquid outlet pipe are arranged on the culture solution bottle; a peristaltic pump; the liquid outlet pipe in the culture solution bottle and one opening end of the cell co-culture unit, the other opening end of the cell co-culture unit and the peristaltic pump, and the peristaltic pump and the liquid inlet pipe in the culture solution bottle form a circulation loop through connecting pipes. The cell co-culture unit is arranged to be in a tubular shape with two open ends and a flowing channel in the middle, the shape of an in-vivo blood vessel is simulated, and the flowing speed of a culture solution is adjusted through a peristaltic pump so as to simulate flowing of fluid in the blood vessel, so that shear stress borne by to-be-cultured cells is close to that in the in-vivo blood vessel; on the basis, the experiment in the haemodynamics aspect is carried out, and the authenticity of the experiment is improved.

Description

Technical Field

[0001] The present invention relates to the field of medical experimental technology, and in particular to a cell co-culture device and method. Background Art

[0002] At present, cell mechanics is a frontier field of biomechanics. Cell mechanics focuses on various cells in the human body, especially those related to the blood circulation system, the human body support and movement system, the digestive system, etc. According to the source of the simulated force, cell mechanics is usually divided into two categories: simulating the in vivo mechanical environment and simulating the in vitro mechanical environment.

[0003] One of the most commonly used models in existing in vitro cell mechanics studies is the parallel plate flow chamber, which is used to simulate the fluid shear stress that various cells in the body are subjected to when they are in a physiological environment and exposed to dynamic liquid flow.

[0004] The metabolic response of cells in vitro is closely related to the wall shear stress they are subjected to. However, the parallel plate flow chamber is a horizontal rectangular chamber that cannot completely simulate the state of blood vessels. Summary of the invention

[0005] The present invention provides a cell co-culture device and method, so as to achieve the purpose of simulating the culture of cells under blood circulation in blood vessels in vitro.

[0006] In order to achieve the above object, the present invention provides a cell co-culture device, comprising:

[0007] A cell co-culture unit, the cell co-culture unit comprising a first culture tube tank and a second culture tube tank, the first culture tube tank and the second culture tube tank enclose a tubular structure with openings at both ends and a flow channel in the middle;

[0008] A culture liquid bottle, wherein the culture liquid bottle is provided with a liquid inlet pipe and a liquid outlet pipe;

[0009] Peristaltic pumps;

[0010] The liquid inlet pipe in the culture liquid bottle and one open end of the cell co-culture unit, the other open end of the cell co-culture unit and the peristaltic pump, the peristaltic pump and the liquid outlet pipe in the culture liquid bottle all form a circulation loop through a connecting pipe.

[0011] Furthermore, the first culture tube trough and the second culture tube trough are both semicircular tube troughs, and the first culture tube trough and the second culture tube trough are connected in a snap-fit ​​connection.

[0012] Furthermore, the cell co-culture device also includes a seal, one end of the seal is an opening, and the other end is provided with a third mounting portion, the seal is installed on the outer walls of the two ends of the first culture tube trough and the second culture tube trough after assembly through the opening, and the third mounting portion is connected to the connecting tube.

[0013] Furthermore, the third mounting portion is the mounting hole, and the connecting pipe is installed in the mounting hole.

[0014] Furthermore, the third mounting portion is the mounting pipe, and the mounting pipe is connected to the connecting pipe via a three-way valve.

[0015] Furthermore, the connecting pipe is a retractable hose, and the connecting pipe sealing sleeve is arranged on the outer side walls of both ends after the first culture tube trough and the second culture tube trough are assembled.

[0016] Furthermore, pressure sensors are provided on the outlet and inlet of the cell co-culture device.

[0017] Furthermore, the culture fluid bottle is also provided with a ventilation port.

[0018] The present invention also provides a cell co-culture method based on the above cell co-culture device, comprising the following steps:

[0019] S1, applying gel on the inner wall of the first culture tube groove and the inner wall of the second culture tube groove;

[0020] S2, spreading the first cells to be cultured onto the gel on the inner wall of the first culture tube groove and the inner wall of the second culture tube groove;

[0021] S3, platting the second cells to be cultured onto the first cells to be cultured in the first culture tube trough and the second culture tube trough;

[0022] S4, assembling the first culture tube tank and the second culture tube tank to form the cell co-culture unit;

[0023] S5, using a connecting hose to connect the liquid inlet tube in the culture liquid bottle to one open end of the cell co-culture unit, the other open end of the cell co-culture unit to the peristaltic pump, and the peristaltic pump to the liquid outlet tube in the culture liquid bottle to form a circulation loop;

[0024] S6. After the connection is completed, the culture solution in the culture solution bottle flows under the action of the peristaltic pump, and the culture solution flows into the flow channel of the cell co-culture unit through the connecting tube, and the culture solution flows out of the flow channel and flows back into the culture solution bottle through the connecting tube.

[0025] Furthermore, the step S1 further includes the following steps:

[0026] S01, preparing four porous polytetrafluoroethylene membranes, and cutting the porous polytetrafluoroethylene membranes to the same area as the inner wall of the first culture tube trough and the second culture tube trough;

[0027] S02. Cultivating the first cells to be cultured on two of the porous polytetrafluoroethylene membranes, and culturing the second cells to be cultured on the other two of the porous polytetrafluoroethylene membranes.

[0028] Compared with the prior art, the cell co-culture device and method of the embodiment of the present invention have the following beneficial effects: the cell co-culture unit in the present invention is enclosed by the first culture tube trough and the second culture tube trough, and the cells to be cultured can be firstly laid flat on the inner wall of the unenclosed first culture tube trough and the second culture tube trough, so as to ensure that the cells to be cultured can be laid flatly and evenly, and then the first culture tube trough and the second culture tube trough are assembled into a cell co-culture unit; the cell co-culture unit is configured as a tubular structure with openings at both ends and a flow channel in the middle to simulate the morphology of blood vessels in the body, and then the flow rate of the culture fluid is adjusted by a peristaltic pump to simulate the flow of fluid in the blood vessels, so that the shear stress to which the cells to be cultured are subjected is similar to that in the blood vessels in the body, and on this basis, hemodynamic experiments can be carried out to improve the authenticity of the experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is the first embodiment of the cell co-culture unit of the embodiment of the present invention;

[0030] Figure 2 This is a second embodiment of the cell co-culture unit of the embodiment of the present invention;

[0031] Figure 3 This is a third embodiment of the cell co-culture unit of the embodiment of the present invention;

[0032] Figure 4 is a schematic structural diagram of a cell co-culture unit according to an embodiment of the present invention;

[0033] Figure 5 Another structural schematic diagram of the cell co-culture unit according to an embodiment of the present invention is

[0034] Figure 6 is a schematic structural diagram of a sealing member according to an embodiment of the present invention;

[0035] Figure 7 is a schematic structural diagram of the installation of a cell co-culture unit and a sealing member according to an embodiment of the present invention;

[0036] Figure 8 The figure is a flow chart of the cell co-culture method of the present invention.

[0037] In the figure, 100, cell co-culture device; 1, cell co-culture unit; 11, first culture tube slot; 111, first mounting portion; 12, second culture tube slot; 121, second mounting portion; 13, flow channel; 2, culture fluid bottle; 21, liquid inlet pipe; 22, liquid outlet pipe; 23, ventilation port; 3, peristaltic pump; 4, connecting pipe; 5, sealing member; 51, opening; 52, third mounting portion; 6, three-way valve. DETAILED DESCRIPTION

[0038] The specific implementation of the present invention is further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0039] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" refers to two or more, unless otherwise clearly and specifically defined.

[0040] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0041] In the present application, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0042] In the application examples, "parallel" means that the angle formed by a straight line, a straight line and a plane, or a plane and a plane is -1° to 1°. In addition, "perpendicular" means that the angle formed by a straight line, a straight line and a plane, or a plane and a plane is 89° to 91°. Equal distance, equal angle or equal area means that the tolerance range is -1% to 1%.

[0043] See also Figures 1 to 7 As shown, a cell co-culture device 100 of the invention embodiment of the present application comprises: a cell co-culture unit 1, a culture fluid bottle 2 and a peristaltic pump 3. The cell co-culture unit 1 comprises a first culture tube slot 11 and a second culture tube slot 12, the first culture tube slot 11 and the second culture tube slot 12 enclose a tubular structure with openings 51 at both ends and a flow channel 13 in the middle; the culture fluid bottle 2 is provided with a liquid inlet pipe 21 and a liquid outlet pipe 22; the peristaltic pump 3 is used to adjust the flow rate of the culture fluid in the culture fluid bottle 2.

[0044] The liquid inlet tube 21 in the culture liquid bottle 2 is connected to one end of the cell co-culture unit 1 through a connecting hose, the other end of the cell co-culture unit 1 is connected to the peristaltic pump 3 through a connecting tube 4, and the other end of the peristaltic pump 3 is connected to the liquid outlet tube 22 of the culture liquid bottle 2 through a connecting hose.

[0045] Specifically, in the present application, the first culture tube trough 11 and the second culture tube trough 12 are both semicircular tube troughs, and the cell co-culture unit 1 assembled by the two is a tubular structure, that is, a hollow cylinder. The cell co-culture unit 1 configured in this way is similar to the structural morphology of a blood vessel and can simulate a blood vessel to conduct hemodynamic experiments.

[0046] The connection mode of the first culture tube tank 11 and the second culture tube tank 12 is a snap-fit ​​connection, wherein the first culture tube tank 11 is provided with a first mounting portion 111, and the second culture tube tank 12 is provided with a second mounting portion 121 assembled with the first mounting portion 111. The first mounting portion 111 and the second mounting portion 121 can be arranged at any position of the connection between the first culture tube tank 11 and the second culture tube tank 12.

[0047] Preferably, the present application is provided with a plurality of first mounting portions 111 and a plurality of second mounting portions 121 at the connection between the first culture tube slot 11 and the second culture tube slot 12. On the one hand, the plurality of first mounting portions 111 and the second mounting portions 121 are snap-fitted and connected, which can ensure the tight and stable connection of the cell co-culture unit 1 and will not be easily separated by external force; on the other hand, when the cell co-culture unit 1 is subjected to external pressure or internal fluid pressure, the plurality of connection structures can help disperse stress and reduce local stress concentration, thereby protecting the integrity of the entire pipeline system.

[0048] The peristaltic pump 3 used in this embodiment is a speed-adjustable peristaltic pump 3, which can adjust the flow rate of the liquid according to the experimental needs. Therefore, in this application, the outlet pipe 22 of the culture liquid bottle 2 is connected to the peristaltic pump 3, and the flow rate of the culture liquid is adjusted by the peristaltic pump 3, and then flows into the flow channel 13 of the cell co-culture unit 1, and then flows through the first cells to be cultured and the second cells to be cultured on the two side walls of the flow channel.

[0049] See also Figure 3 As shown, in this embodiment, the connecting pipe 4 is a retractable hose, and the connecting pipe 4 is sealed and sleeved on the outer side walls of both ends of the first culture tube tank 11 and the second culture tube tank 12 after assembly.

[0050] In the present application, the culture medium bottle 2 is filled with culture medium, and a liquid inlet pipe 21 and a liquid outlet pipe 22 are arranged on the culture medium bottle 2. The length of the liquid outlet pipe 22 in the culture medium bottle 2 is longer than that of the liquid inlet pipe 21. The longer liquid outlet pipe 22 can better control the flow path of the culture medium, help the culture medium to form an effective circulation flow inside the container, and improve the mixing effect.

[0051] Furthermore, the culture liquid bottle 2 is also provided with a ventilation port 23. In some cases, the outflow and inflow of the culture liquid may cause the pressure in the culture liquid bottle 2 to change, which may affect the stability of the flow of the culture liquid. The ventilation port 23 can help maintain the pressure balance inside and outside the culture liquid bottle 2. At the same time, the organism will produce carbon dioxide during the metabolism process. Excess carbon dioxide will cause the pH value in the bottle to decrease, which is not conducive to the growth of the organism. Excess carbon dioxide can be discharged through the ventilation port 23.

[0052] Furthermore, pressure sensors (not shown) are provided on the outlet and inlet ends of the cell co-culture device 100 . The pressure sensors can measure the shear force of the liquid at both ends of the cell co-culture unit 1 , and then adjust the flow rate of the liquid through the peristaltic pump 3 .

[0053] See also Figure 2As shown, in the second embodiment of the present application, the cell co-culture device 100 further includes a sealing member 5, one end of the sealing member 5 is an opening 51, and the other end is provided with a third mounting portion 52, the sealing member 5 is mounted on the outer side walls of both ends of the first culture tube tank 11 and the second culture tube tank 12 after assembly through the opening 51, and the third mounting portion 52 is connected to the connecting tube 4. The third mounting portion 52 is a mounting hole, and the connecting tube 4 is mounted in the mounting hole.

[0054] See also Figure 1 As shown, in the third embodiment of the present application, the third mounting portion 52 is a mounting tube, and the mounting tube is connected to the connecting tube 4 via a three-way valve 6. The three-way valve 6 in the present application can connect or disconnect the connecting tube 4 and the flow channel 13 of the cell co-culture unit 1, and can control whether the culture fluid in the culture fluid bottle 2 flows into the flow channel 13, and can also control the speed at which the culture fluid flows into the flow channel 13, so as to make different adjustments according to different experimental requirements.

[0055] See also Figure 8 As shown, the present invention also provides a cell co-culture method based on the cell co-culture device 100, comprising the following steps:

[0056] S1, applying gel on the inner wall of the first culture tube trough 11 and the inner wall of the second culture tube trough 12;

[0057] S2, spreading the first cells to be cultured onto the gel on the inner wall of the first culture tube trough 11 and the inner wall of the second culture tube trough 12;

[0058] S3, platting the second cells to be cultured onto the first cells to be cultured in the first culture tube tank 11 and the second culture tube tank 12;

[0059] S4, assembling the first culture tube tank 11 and the second culture tube tank 12 to form the cell co-culture unit 1;

[0060] S5, using a connecting hose to connect the liquid inlet tube 21 in the culture liquid bottle 2 to an opening 51 end of the cell co-culture unit 1, the other opening 51 end of the cell co-culture unit 1 to the peristaltic pump 3, and the peristaltic pump 3 to the liquid outlet tube 22 in the culture liquid bottle 2 to form a circulation loop;

[0061] S6. After the connection is completed, the culture solution in the culture solution bottle 2 flows under the action of the peristaltic pump 3, and the culture solution flows into the flow channel 13 of the cell co-culture unit 1 through the connecting tube 4. The culture solution flows out of the flow channel 13 and flows back into the culture solution bottle 2 through the connecting tube 4.

[0062] In the present application, the first cells to be cultured are smooth muscle cells, and the second cells to be cultured are endothelial cells. The present application can be used to conduct experiments on hemodynamics.

[0063] In S1, gel is applied to the inner wall of the first culture tube trough 11 and the inner wall of the second culture tube trough 12. The purpose of applying gel is to enable the first cells to be cultured and the second cells to be cultured to be firmly attached to the inner wall of the first culture tube trough 11 and the inner wall of the second culture tube trough 12. The gel is equivalent to the connective tissue in the blood vessels and provides support.

[0064] In S3, the second cells to be cultured are plated on the first cells to be cultured in order to form a symbiotic state between the first cells to be cultured and the second cells to be cultured.

[0065] In S4, the first culture tube trough 11 and the second culture tube trough 12 are assembled into a cell co-culture unit 1, that is, assembled into a structure similar to a blood vessel to simulate a blood vessel for experiment.

[0066] S5 is to connect all components to form a circulation loop required for the experiment. It should be noted that during the connection process, the outlet pipe 22 of the culture liquid bottle 2 needs to be connected to the peristaltic pump 3, because only in this way can the culture liquid in the culture liquid bottle 2 be adjusted by the peristaltic pump 3 and flow out of the peristaltic pump 3 at a desired speed, thereby flowing into the cell co-culture unit 1.

[0067] In the first embodiment of the present application, S5 directly connects the connecting hose to both ends of the cell co-cultivation unit 1;

[0068] In the second and third embodiments of the present application, sealing members 5 are installed at both ends of the cell co-culture unit 1, and the other side of the sealing member 5 is connected to the connecting tube 4; the cell co-culture unit 1 is connected to the peristaltic pump 3 through the sealing member 5 and the connecting tube 4, and the other end of the peristaltic pump 3 is connected to the liquid inlet pipe 21 of the culture fluid bottle 2 through the connecting tube 4; the liquid outlet pipe 22 of the culture fluid bottle 2 is connected to the other end of the cell co-culture unit 1 through the connecting tube 4 and the sealing member 5.

[0069] In the second embodiment, the end of the seal 5 directly connected to the cell co-culture unit 1 is an opening 51, and the end connected to the connecting hose is a mounting hole, and the connecting tube 4 is installed in the mounting hole. The seal 5 is used to ensure that the positions of the first culture tube slot 11 and the second culture tube slot 12 are fixed, so that the two fit closely, providing a basis for simulating the shape and structure of the blood vessel;

[0070] In the third embodiment, the end of the seal 5 directly connected to the cell co-culture unit 1 is an opening 51, and the end connected to the connecting hose is a mounting tube. The mounting tube is connected to the connecting tube 4 via a three-way valve 6. By using the three-way valve 6, the inflow of the culture fluid into and out of the flow channel 13 can be controlled and regulated. The use of the seal 5 ensures that the positions of the first culture tube trough 11 and the second culture tube trough 12 are fixed, so that the two fit closely, providing a basis for simulating the shape and structure of blood vessels.

[0071] The following steps are also included before S1:

[0072] S01, preparing four porous polytetrafluoroethylene membranes, and cutting the porous polytetrafluoroethylene membranes to the same area as the inner wall of the first culture tube trough 11 and the second culture tube trough 12;

[0073] S02. Cultivating the first cells to be cultured on two of the porous polytetrafluoroethylene membranes, and culturing the second cells to be cultured on the other two of the porous polytetrafluoroethylene membranes.

[0074] Specifically, porous polytetrafluoroethylene membranes have good air permeability, allowing oxygen and carbon dioxide to pass freely, which is essential for maintaining the health of cells. Cells need oxygen to carry out metabolic activities during the culture process, and they need to eliminate the carbon dioxide produced by metabolism. The properties of polytetrafluoroethylene are generally considered to be biocompatible, which means that it does not cause obvious immune or toxic reactions and is suitable for biomedical research and cell culture. At the same time, the porous structure can simulate the three-dimensional environment in which cells grow in the body, which helps cells to better attach and proliferate. The porous membrane can also be used as a scaffold material for constructing tissue engineering products to promote the three-dimensional growth of cells, where the pore size can be set as needed.

[0075] The working process of the present invention is as follows: seals 5 are installed at both ends of the cell co-culture unit 1, and the other side of the seals 5 is connected to the connecting tube 4; the cell co-culture unit 1 is connected to the peristaltic pump 3 through the seals 5 and the connecting tube 4, and the other end of the peristaltic pump 3 is connected to the liquid inlet pipe 21 of the culture liquid bottle 2 through the connecting tube 4; the liquid outlet pipe 22 of the culture liquid bottle 2 is connected to the other end of the cell co-culture unit 1 through the connecting tube 4 and the seals 5. After the connection is completed, the culture liquid in the culture liquid bottle 2 flows under the action of the peristaltic pump 3, and the culture liquid flows into the flow channel 13 of the cell co-culture unit 1 through the connecting tube 4, and the culture liquid flows out from the flow channel 13 and flows back to the culture liquid bottle 2 through the connecting tube 4.

[0076] In summary, the embodiments of the present invention provide a cell co-culture device and method, wherein the cell co-culture unit is enclosed by a first culture tube trough and a second culture tube trough, and the cells to be cultured can be firstly laid flat on the inner walls of the unenclosed first culture tube trough and the second culture tube trough, thereby ensuring that the cells to be cultured can be laid flatly and evenly, and then the first culture tube trough and the second culture tube trough are assembled into a cell co-culture unit; the cell co-culture unit is configured as a tubular structure with openings at both ends and a flow channel in the middle to simulate the morphology of blood vessels in the body, and then the flow rate of the culture fluid is adjusted by a peristaltic pump to simulate the flow of fluid in the blood vessels, so that the shear stress to which the cells to be cultured are subjected is similar to that in the blood vessels in the body, and on this basis, hemodynamic experiments can be carried out to improve the authenticity of the experiment.

[0077] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and substitutions can be made without departing from the technical principles of the present invention. These improvements and substitutions should also be regarded as the scope of protection of the present invention.

Claims

1. A cell co-culture device, characterized in that: include: A cell co-culture unit, the cell co-culture unit comprising a first culture tube tank and a second culture tube tank, the first culture tube tank and the second culture tube tank enclose a tubular structure with openings at both ends and a flow channel in the middle; A culture liquid bottle, wherein the culture liquid bottle is provided with a liquid inlet pipe and a liquid outlet pipe; Peristaltic pumps; The liquid inlet pipe in the culture liquid bottle and one open end of the cell co-culture unit, the other open end of the cell co-culture unit and the peristaltic pump, the peristaltic pump and the liquid outlet pipe in the culture liquid bottle all form a circulation loop through a connecting pipe.

2. The cell co-culture device according to claim 1, characterized in that: The first culture tube slot and the second culture tube slot are both semicircular tube slots, and the first culture tube slot and the second culture tube slot are connected in a snap-fit ​​connection.

3. The cell co-culture device according to claim 1, characterized in that: The cell co-culture device also includes a seal, one end of which is an opening and the other end of which is provided with a third mounting portion. The seal is mounted on the outer side walls of the first culture tube trough and the second culture tube trough after assembly through the opening, and the third mounting portion is connected to the connecting tube.

4. The cell co-culture device according to claim 3, characterized in that: The third mounting portion is a mounting hole, and the connecting pipe is mounted in the mounting hole.

5. The cell co-culture device according to claim 3, characterized in that: The third mounting portion is a mounting pipe, and the mounting pipe is connected to the connecting pipe via a three-way valve.

6. The cell co-culture device according to claim 1, characterized in that: The connecting pipe is a retractable hose, and the connecting pipe sealing sleeve is arranged on the outer side walls of both ends of the first culture tube tank and the second culture tube tank after they are assembled.

7. The cell co-culture device according to claim 1, characterized in that: Pressure sensors are provided on the outlet and inlet ends of the cell co-culture device.

8. The cell co-culture device according to claim 1, characterized in that: The culture fluid bottle is also provided with a ventilation port.

9. A cell co-culture method based on the cell co-culture device according to any one of claims 1 to 8, characterized in that: The steps include: S1, applying gel on the inner wall of the first culture tube groove and the inner wall of the second culture tube groove; S2, spreading the first cells to be cultured onto the gel on the inner wall of the first culture tube groove and the inner wall of the second culture tube groove; S3, platting the second cells to be cultured onto the first cells to be cultured in the first culture tube trough and the second culture tube trough; S4, assembling the first culture tube tank and the second culture tube tank to form the cell co-culture unit; S5, using a connecting hose to connect the liquid inlet tube in the culture liquid bottle to one open end of the cell co-culture unit, the other open end of the cell co-culture unit to the peristaltic pump, and the peristaltic pump to the liquid outlet tube in the culture liquid bottle to form a circulation loop; S6. After the connection is completed, the culture solution in the culture solution bottle flows under the action of the peristaltic pump, and the culture solution flows into the flow channel of the cell co-culture unit through the connecting tube, and the culture solution flows out of the flow channel and flows back into the culture solution bottle through the connecting tube.

10. The cell co-culture method according to claim 9, characterized in that: The S1 also includes the following steps: S01, preparing four porous polytetrafluoroethylene membranes, and cutting the porous polytetrafluoroethylene membranes to the same area as the inner wall of the first culture tube trough and the second culture tube trough; S02. Cultivating the first cells to be cultured on two of the porous polytetrafluoroethylene membranes, and culturing the second cells to be cultured on the other two of the porous polytetrafluoroethylene membranes.

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