Device and method for detecting various forbidden fishery medicines based on Raman spectrum and microfluidic technology

Abstract

The invention discloses a device and a method for detecting various forbidden fishery drugs based on Raman spectrum and micro-fluidic technology, the device comprises an automatic sample injection module, a micro-fluidic mixing module, a Raman data acquisition module, a data processing module and a display module, the automatic sample injection module is used for automatically distributing various reagents and injecting a water sample to be detected; the microfluidic mixing module receives and uniformly mixes various reagents and a to-be-detected fish medicine sample to form a mixed sample solution and outputs the mixed sample solution, the Raman data acquisition module emits laser to the mixed sample solution, acquires a Raman scattering signal generated by the mixed sample solution under laser irradiation, and sends the Raman scattering signal to the data processing module after photoelectric conversion and primary treatment; the data processing module identifies and quantifies forbidden fishery medicine components and contents in the sample, and the display module displays a detection result, an analysis report and state information of the detection device. According to the invention, the problems of insufficient application scenes and low detection efficiency of existing water quality multi-factor parameter detection equipment can be solved.

Description

Technical Field

[0001] The invention relates to a water environment pollution monitoring technology, and in particular to a device and method for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology. Background Art

[0002] As the scale and intensiveness of aquaculture continue to increase, aquatic products are prone to health problems that affect production. In pursuit of profit, some farmers often abuse banned fishery drugs during the breeding process. This behavior not only seriously affects the quality of aquatic products and consumer health, but also poses a threat to the safety of the agricultural ecological environment.

[0003] Existing detection methods have a long cycle, high requirements on manpower and instruments, and have problems such as poor data timeliness, single detection target, and low detection efficiency. Automated detection sensors are still in their infancy and cannot meet actual detection needs. Summary of the invention

[0004] Purpose of the invention: The purpose of the present invention is to provide a device and method for detecting a variety of banned fishery drugs based on Raman spectroscopy and microfluidics technology, which can solve the problems of insufficient application scenarios and low detection efficiency of current water quality multi-factor parameter detection equipment.

[0005] Technical solution: The present invention discloses a device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidic technology, comprising an automatic sampling module, a microfluidic mixing module, a Raman data acquisition module, a data processing module and a display module; the automatic sampling module is used for automatically dispensing multiple reagents and sampling water samples to be detected, the microfluidic mixing module is connected to the automatic sampling module, and is used to receive multiple reagents and water samples to be detected from the automatic sampling module and mix them evenly to form a mixed sample liquid and output it, the Raman data acquisition module is used to emit laser to the mixed sample liquid output by the microfluidic mixing module, and collect Raman scattering signals generated by the mixed sample liquid under laser irradiation, the Raman scattering signals are sent to the data processing module for analysis after photoelectric conversion and preliminary processing, the data processing module is used to identify and quantify the banned fishery drug components and contents in the sample, and the display module is connected to the data processing module, and is used to display the detection results, analysis reports and status information of the detection device.

[0006] Furthermore, the microfluidic mixing module includes one or more microfluidic chips, and a plurality of reagents are uniformly mixed in the channels inside the microfluidic chips to form a mixed sample liquid.

[0007] Furthermore, a spiral or serpentine microchannel is provided inside the microfluidic chip to increase the mixing path of the liquid, improve the mixing uniformity, and reduce the consumption of the sample.

[0008] Furthermore, the Raman data acquisition module includes a laser transmitter, a Raman spectrometer connected to the laser transmitter, and a Raman probe connected to the output end of the Raman spectrometer. The laser transmitter is used to emit laser to the mixed sample liquid output by the microfluidic mixing module. The Raman spectrometer is used to collect Raman scattering signals generated by the mixed sample liquid under laser irradiation. The Raman probe is used to receive the Raman scattering signals and convert them into electrical signals, which are then processed to form Raman spectrum data.

[0009] Furthermore, the data processing module includes an embedded computing platform, and the processor inside the embedded computing platform integrates multiple algorithm libraries for real-time processing of Raman spectral data formed after preliminary processing by the Raman data acquisition module, and detecting the banned fishery drug components and content in the sample based on the fishery drug detection algorithm.

[0010] Furthermore, the automatic sampling module includes a liquid storage tank, a vertical injection pump and a multi-channel rotary valve connected to the liquid storage tank through a pipeline.

[0011] Furthermore, the vertical syringe pump has a high-precision stepper motor drive system, which controls the quantitative aspiration and injection of the sample liquid and automatically adjusts the volume of the liquid aspirated each time according to a preset program, and can achieve a minimum injection accuracy of 0.4 μL.

[0012] Furthermore, the multi-channel rotary valve includes a plurality of independent reagent channels, and each channel is provided with a sealing device to prevent cross contamination between reagents.

[0013] Furthermore, the multi-channel rotary valve realizes a programmable control function through a host computer or an independent electronic control module, thereby realizing the selection of different reagent channels according to different detection requirements.

[0014] Based on the same inventive concept, a method for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology of the present invention is applied to the above-mentioned multiple banned fishery drug detection device based on Raman spectroscopy and microfluidics technology, comprising the following steps:

[0015] The automatic injection module injects the water sample to be tested and automatically distributes multiple reagents according to the preset ratio to complete the automatic injection of reagents;

[0016] The water sample to be tested and the corresponding reagents enter the microfluidic mixing module, and the microfluidic mixing module evenly mixes the water sample to be tested and the corresponding reagents to form a mixed sample liquid and outputs it;

[0017] The Raman data acquisition module emits laser to the mixed sample liquid output by the microfluidic mixing module to collect the Raman scattering signal generated by the mixed sample liquid under laser irradiation. The Raman scattering signal is sent to the data processing module for analysis after photoelectric conversion and preliminary processing.

[0018] The data processing module identifies and quantifies the banned fishery drug components and content in the samples;

[0019] The display module displays the test results, analysis reports and status information of the test device.

[0020] Beneficial effects: Compared with the prior art, the remarkable technical effects of the present invention are as follows: the use of an independently designed microfluidic mixing module and a microfluidic chip as a mixing and reaction platform significantly reduces the demand for reagents in the detection process, thereby reducing the detection cost and greatly reducing the risk of additional waste liquid generation and environmental pollution, and improving the green environmental protection and sustainability of the detection process; through the integration of real-time acquisition and processing technology of spectral data, Raman spectral data can be quickly and accurately acquired and analyzed, and the composition and content of banned fishery drugs in samples can be accurately identified, solving the technical problems of low detection efficiency and insufficient sensitivity of existing detection methods; in addition, the test results can be intuitively displayed and shared through touch screen, printout or remote communication, which greatly improves the operating convenience and scope of application of the equipment, thereby realizing efficient, accurate and automated detection of banned fishery drugs, and providing strong technical support for fishery supervision. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A working principle diagram of a device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology disclosed in an embodiment of the present invention;

[0022] Figure 2 A front schematic diagram of a device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology disclosed in an embodiment of the present invention;

[0023] Figure 3 This is a schematic diagram of the back side of a device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology disclosed in an embodiment of the present invention. DETAILED DESCRIPTION

[0024] The technical solution of the present invention is described in detail below in conjunction with specific implementation methods and the accompanying drawings of the specification.

[0025] Example 1

[0026] like Figure 1As shown, the device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidic technology of the present invention comprises an automatic sampling module, a microfluidic mixing module, a Raman data acquisition module, a data processing module and a display module. The automatic sampling module is used for the automatic distribution of multiple reagents and the sampling of water samples to be detected. The microfluidic mixing module is connected to the automatic sampling module, and is used to receive multiple reagents and water samples to be detected from the automatic sampling module and mix them evenly to form a mixed sample liquid and output it. The Raman data acquisition module is used to emit laser to the mixed sample liquid output by the microfluidic mixing module, and collect the Raman scattering signal generated by the mixed sample liquid under laser irradiation. The Raman scattering signal is sent to the data processing module for analysis after photoelectric conversion and preliminary processing. The data processing module is used to identify and quantify the banned fishery drug components and contents in the sample. The display module is connected to the data processing module, and is used to display the detection results, analysis reports and status information of the detection device.

[0027] like Figure 2 As shown, the automatic sampling module includes a multi-channel rotary valve 6, a vertical injection pump 7, and a liquid storage tank 12. The vertical injection pump 7 and the multi-channel rotary valve 6 are respectively connected to the liquid storage tank 12 through pipelines.

[0028] Among them, the vertical syringe pump 7 has a high-precision stepper motor drive system, which can achieve a minimum injection accuracy of 0.4μL and automatically adjust the volume of liquid absorbed each time according to a preset program, thereby realizing quantitative absorption and injection of the sample liquid, and the injection error range does not exceed the adjustable threshold set by the system.

[0029] The multi-channel rotary valve 6 includes several independent reagent channels, and each channel is provided with a sealing device. The multi-channel rotary valve 6 realizes a programmable control function through a host computer or an independent electronic control module, so as to select different reagent channels according to different detection requirements.

[0030] In this embodiment, the multi-channel rotary valve includes at least 15 independent reagent channels, each of which is provided with a sealing device to prevent cross-contamination between reagents; the multi-channel rotary valve has a programmable control function, which is realized by a host computer or an independent electronic control module, and can flexibly select different reagent channels according to different detection requirements, and monitor the flow and status of the reagents in real time through a built-in feedback system to ensure the stability and reliability of the injection process.

[0031] The microfluidic mixing module includes one or more microfluidic chips, and multiple reagents are evenly mixed in the channels inside the microfluidic chip. The microfluidic chip is made of a polymer material with good chemical stability and thermal stability, preferably PDMS or PMMA material, and a spiral or serpentine microchannel structure is designed inside the chip to increase the mixing path of the liquid, improve the mixing uniformity, and reduce the consumption of samples.

[0032] The microfluidic chip can adapt to the flow and reaction environment of various required detection reagents. The chip is designed as a detachable and replaceable modular structure. It can achieve flexible mixing of different reagents through standardized interfaces or fixtures, and ensure the sealing and functionality after multiple uses.

[0033] The Raman data acquisition module includes a laser emitter 5, a Raman spectrometer 3 connected to the laser emitter 5, and a Raman probe 14 connected to the output end of the Raman spectrometer 3. The laser emitter 5 is used to emit laser to the mixed sample liquid output by the microfluidic mixing module. The Raman spectrometer 3 is used to collect the Raman scattering signal generated by the mixed sample liquid under laser irradiation. The Raman probe 14 is used to receive the Raman scattering signal and convert it into an electrical signal, and form Raman spectrum data after preliminary processing.

[0034] The data processing module includes an embedded computing platform 15. The processor inside the embedded computing platform 15 integrates multiple algorithm libraries for real-time processing of Raman spectral data formed after preliminary processing by the Raman data acquisition module, and detects the banned fishery drug components and contents in the sample based on the fishery drug detection algorithm.

[0035] In this embodiment, the data processing module adopts an embedded computing platform based on the x86 architecture, equipped with a high-performance processor, a readable storage medium and multiple interface resources for data transmission and inter-module communication; the platform integrates a variety of algorithm libraries, which can perform real-time analysis, background correction, feature extraction and model prediction on the collected Raman spectral data, support batch processing of complex data sets, and achieve rapid output of results.

[0036] The display module is equipped with a touch screen and a multi-language operation interface, allowing users to set parameters, query results and export reports through simple operations to enhance the user-friendliness and applicability of the equipment.

[0037] A device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology is introduced below through a specific embodiment.

[0038] like Figure 2 and Figure 3 As shown, in a specific embodiment, the device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidic technology of the present invention includes a chassis shell 1, a Raman fixed horizontal plate 2, a Raman spectrometer 3, a laser fixed horizontal plate 4, a 785nm laser transmitter 5, a multi-channel rotary valve 6, a vertical injection pump 7, a power control box 8, a Raman probe fixing fixture 9, a microfluidic fixing fixture 10, a front panel 11, a liquid storage tank 12, a glass observation window 13, a Raman probe 14, an embedded computing platform 15, a touch panel 16, and a power switch 17.

[0039] A Raman spectrometer 3 is fixedly mounted on the Raman fixed horizontal plate 2 on the upper part of the chassis housing 1, and its output end is connected to the Raman probe 14, and the input end of the Raman spectrometer 3 is connected to the laser transmitter 5, and the output end of the Raman spectrometer 3 is connected to the embedded computing platform 15, and a separate power port is used for power supply, and a heat dissipation hole is provided at the rear of the Raman spectrometer 3. The laser fixed horizontal plate 4 is used to fix the laser transmitter 5ADR-1805 with a laser wavelength of 785nm. The laser transmitter 5 has a separate switch and power adjustment button and needs to be powered separately. The Raman probe 14 is fixed by the Raman probe fixing fixture 9, and the height can be adjusted up and down by the wire shaft to complete the focus. The microfluidic fixing fixture 10 is fixedly installed directly below the probe to fix the microfluidic chip, fix the microfluidic chip and leave a threaded connector connected to the multi-position valve and the waste liquid tank. The power control box 8 is four national standard three-plugs to power the Raman instrument and the injection pump. The upper half of the chassis is encapsulated and hidden by the front panel 11. The touch panel 16 is installed behind the panel for personnel to interact with the entire device and display the results. The embedded computing platform 15 is also hidden and installed behind the panel. The liquid storage tank 12 for the reagent is fixed in the lower left corner of the chassis. The bottom of the liquid storage tank 12 is a threaded hole, which is connected to the multi-channel rotary valve 6 and the vertical injection pump 7 through a Teflon tube. There is a glass observation window 13 on the front door of the chassis, and a power switch 17 is installed on the right.

[0040] In actual application, the above-mentioned multiple banned fishery drug detection device based on Raman spectroscopy and microfluidics includes two working processes: liquid inlet calibration detection and liquid discharge cleaning.

[0041] The entire test process is controlled by the host computer interface. The entire process of liquid inlet calibration test includes calibration using 9 standard reagents, involving the mixing and detection of standard reagents and enhancement liquid reagents.

[0042] The host computer control end sends a start command, and the multi-position valve switches the channels to the standard liquid channel 1, the enhancement liquid channels 1, 2, and 3 in turn. The four reagents are sucked in through the syringe pump and injected back into the microfluidic chip to complete the reagent mixing in the microchannel.

[0043] The height of the Raman probe and the microfluidic detection cell is adjusted in advance by the thread shaft slide bar to complete the focus, and the slide bar is fixed. The host computer control end continues to send a command to turn on the laser, and the 785nm laser transmitter 5 emits laser to the sample, and the Raman probe 14 receives the scattered light data. The data is converted into an electrical signal by the portable Raman spectrometer 3 coupled to the Raman probe and transmitted to the embedded computing platform 15 in the chassis to complete a calibration.

[0044] Before the next calibration, the entire flow path including the microfluidic chip needs to be cleaned and drained. By switching the multi-position valve channel to the deionized water channel, deionized water is drawn to flush the entire flow path including the microfluidic and liquid storage rings to prepare for the next calibration.

[0045] Repeat the above steps to complete the calibration of 9 standard solutions and the Raman data collection of the water sample to be tested, and extract the spectral data as the input features of the model. Then, based on the characteristic spectrum data, a regression model of the fishery drug content is constructed, and a reasonable warning line is set to judge whether the fishery drug content has reached the warning level.

[0046] For the Raman spectra of mixed fishery drugs, the deconvolution method is used to eliminate the peak overlap caused by instrument broadening, and the mean of the Raman spectrum of a single fishery drug is superimposed with random coefficients and noise to establish a Raman spectrum data set of mixed fishery drugs. The quantitative detection model and PLS-DA algorithm are used to calculate the Raman spectra of mixed fishery drugs in complex scenes. Finally, the above-mentioned fishery drug detection model is used to realize the quantitative detection of fishery drugs in a complex system of mixed fishery drugs.

[0047] After the test process is completed, the processed data is transmitted to the LCD display interface through the data processing module, providing users with an intuitive display of the test results. The interface also provides a detailed test report area for displaying more comprehensive test results. The report content includes but is not limited to: test time, sample number, test parameter settings, spectrum comparison, risk assessment level, etc. These detailed information provide users with a comprehensive test review and analysis basis. In addition, users can perform simple operations through the touch screen, such as switching to view the test results of different samples, adjusting display parameters, and exporting test reports to external storage devices.

[0048] The present invention realizes automatic absorption and injection of multiple reagents by controlling the injection pump, and utilizes the microchannel structure inside the microfluidic chip to realize the mixing and reaction of reagents. Subsequently, a portable Raman spectrometer is used to perform rapid, non-contact detection on the mixed sample, directly obtain the Raman spectrum characteristic information of the banned fishery drugs in the sample, and realize the detection of multiple target fishery drug contents and excessive warning. The device is equipped with a host computer system and an interactive interface to provide an intuitive display of the test results. The present invention realizes the automation and integration of the fishery drug detection device, significantly improves the detection efficiency and accuracy, is suitable for on-site environments such as aquaculture farms, and provides reliable technical support for the supervision of banned fishery drugs.

[0049] Example 2

[0050] This embodiment includes a method for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology. The method is applied to a device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology in Example 1, and includes the following steps:

[0051] S1. The automatic injection module injects the water sample to be tested and automatically distributes multiple reagents according to the preset ratio to complete the automatic injection of reagents.

[0052] The automatic injection module is controlled and distributed by a vertical syringe pump and a multi-channel rotary valve to complete the automated injection of reagents, and mix the water sample to be tested with the corresponding reagent in a preset ratio.

[0053] S2. The water sample to be tested and the corresponding reagents enter the microfluidic mixing module, and the microfluidic mixing module evenly mixes the water sample to be tested and the corresponding reagents to form a mixed sample liquid and outputs it.

[0054] The injected samples and reagents are transported to the microfluidic chip and mixed using the micro-mixing structure inside the microfluidic chip to ensure that the samples and reagents are in full contact and produce the expected chemical reaction or physical effect.

[0055] The preset program in the embedded computing platform controls the injection pump to cooperate with the multi-position valve to extract standard reagents and enhanced sample solutions from the liquid storage tank in sequence, inject them into the microfluidic chip through the pipeline, and realize the mixing of reagents in the microfluidic microchannel.

[0056] S3, the Raman data acquisition module emits laser to the mixed sample liquid output by the microfluidic mixing module, collects the Raman scattering signal generated by the mixed sample liquid under laser irradiation, and the Raman scattering signal is sent to the data processing module for analysis after photoelectric conversion and preliminary processing.

[0057] The small portable Raman spectrometer with the Raman data acquisition module is started. The laser light source irradiates the mixed sample output by the microfluidic mixing module, and the generated Raman scattered light is received by the photodetector and converted into an electrical signal. The signal is then processed initially, including amplification, filtering, and analog-to-digital conversion, to generate Raman spectral data that can be used for subsequent analysis.

[0058] The laser is controlled to emit laser to the mixed reagent sample, and the scattered light signal is received through the Raman probe, and the scattered light signal is converted into an electrical signal and transmitted to the embedded platform for processing.

[0059] Continuously switch the multi-position valve channel and repeat steps S1-S3 to establish a standard line with the standard solution.

[0060] The multi-position valve is switched to the test solution channel and the corresponding enhancement solution channel to extract the sample solution and complete the mixing in the microfluidics.

[0061] The laser is controlled to emit laser to the sample of the test agent, and the scattered light signal is received through the Raman probe, and the scattered light signal is converted into an electrical signal and transmitted to the embedded platform for processing.

[0062] S4. The data processing module identifies and quantifies the banned fishery drug components and contents in the samples.

[0063] The Raman spectral data is transmitted to the embedded computing platform of the data processing module for real-time data processing, including background correction, noise filtering, feature extraction and modeling prediction. Based on the established fishery drug detection algorithm, analysis reports and prediction results are generated.

[0064] The embedded platform predicts the fishery drug components in the water samples to be tested based on the quantitative detection model established based on local data.

[0065] S5. The display module displays the test results, analysis report and status information of the test device.

[0066] The test results, analysis reports and system status information are displayed through the result output display module, including intuitive display on the touch screen display, printout by printer, and data sharing with remote servers or mobile devices through the wireless communication interface.

[0067] The technical solution of the present invention realizes the extraction of different sample reagents and completes the mixing in the microfluidics through the cooperation of the injection pump and the multi-position valve, takes the microfluidic chip as the stage, utilizes the Raman spectroscopy detection system to collect the spectral data of the water quality of the laser-irradiated sample and transmits it to the embedded platform, performs water quality detection based on the original spectral data and the pre-trained water quality detection model, determines the corresponding multiple target fishery medicine information in the target water sample and displays it.

Claims

1. A device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology, characterized in that: It includes automatic sampling module, microfluidic mixing module, Raman data acquisition module, data processing module and display module; The automatic sampling module is used for automatic distribution of multiple reagents and sampling of water samples to be tested. The microfluidic mixing module is connected to the automatic sampling module, and is used to receive multiple reagents and water samples to be tested from the automatic sampling module and mix them evenly to form a mixed sample liquid and output it. The Raman data acquisition module is used to emit laser to the mixed sample liquid output by the microfluidic mixing module, and collect Raman scattering signals generated by the mixed sample liquid under laser irradiation. The Raman scattering signals are sent to the data processing module for analysis after photoelectric conversion and preliminary processing. The data processing module is used to identify and quantify the banned fishery drug components and contents in the sample. The display module is connected to the data processing module, and is used to display the test results, analysis reports and status information of the detection device.

2. The device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology according to claim 1 is characterized in that: The microfluidic mixing module includes one or more microfluidic chips, and multiple reagents are uniformly mixed in the channels inside the microfluidic chips.

3. The device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology according to claim 2 is characterized in that: A spiral or serpentine microchannel is arranged inside the microfluidic chip.

4. The device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology according to claim 1 is characterized in that: The Raman data acquisition module comprises a laser emitter (5), a Raman spectrometer (3) connected to the laser emitter (5), and a Raman probe (14) connected to the output end of the Raman spectrometer (3); the laser emitter (5) is used to emit laser light to the mixed sample liquid output by the microfluidic mixing module; the Raman spectrometer (3) is used to collect Raman scattering signals generated by the mixed sample liquid under laser irradiation; the Raman probe (14) receives the Raman scattering signals and converts them into electrical signals, and forms Raman spectrum data after preliminary processing.

5. The device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology according to claim 1 is characterized in that: The data processing module comprises an embedded computing platform (15), wherein a processor inside the embedded computing platform (15) integrates a plurality of algorithm libraries for real-time processing of Raman spectrum data generated after preliminary processing by the Raman data acquisition module, and detecting the prohibited fishery drug components and contents in the sample based on the fishery drug detection algorithm.

6. The device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology according to claim 1, characterized in that: The automatic sampling module comprises a liquid storage tank (12), a vertical injection pump (7) and a multi-channel rotary valve (6) connected to the liquid storage tank (12) via a pipeline.

7. The device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology according to claim 6 is characterized in that: The vertical injection pump (7) has a high-precision stepper motor drive system, which controls the quantitative absorption and injection of the sample liquid and automatically adjusts the volume of the liquid absorbed each time according to a preset program.

8. The device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology according to claim 6 is characterized in that: The multi-channel rotary valve (6) comprises a plurality of independent reagent channels, and each channel is provided with a sealing device.

9. The device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology according to claim 8, characterized in that: The multi-channel rotary valve (6) realizes a programmable control function through a host computer or an independent electronic control module, thereby realizing the selection of different reagent channels according to different detection requirements.

10. A method for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology, characterized in that: The method is applied to a device for detecting multiple banned fishery drugs based on Raman spectroscopy and microfluidics technology as described in claim 1, comprising the following steps: The automatic injection module injects the water sample to be tested and automatically distributes multiple reagents according to the preset ratio to complete the automatic injection of reagents; The water sample to be tested and the corresponding reagents enter the microfluidic mixing module, and the microfluidic mixing module evenly mixes the water sample to be tested and the corresponding reagents to form a mixed sample liquid and outputs it; The Raman data acquisition module emits laser to the mixed sample liquid output by the microfluidic mixing module to collect the Raman scattering signal generated by the mixed sample liquid under laser irradiation. The Raman scattering signal is sent to the data processing module for analysis after photoelectric conversion and preliminary processing. The data processing module identifies and quantifies the banned fishery drug components and content in the samples; The display module displays the test results, analysis reports and status information of the test device.

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