Full-automatic double-lens microscopic detection observation device and method

Abstract

The invention discloses a full-automatic double-lens microscopic detection observation device and a full-automatic double-lens microscopic detection observation method, relates to the field of intelligent control, and solves the problems that only a limited position can be observed and a result cannot be accurately analyzed due to the fact that a traditional observation method adopts a mode of moving a culture dish in a single transmission small range for observation. The device comprises a top device and a bottom device, wherein the top device comprises a main control module and an upper movement module; the bottom device comprises a lower movement module and an optical module; the upper motion module is arranged on a bracket of the observation device; the optical module comprises a light source, a light source fixture, a camera, a light source adjusting block and a light source fixing ring; the light source clamp is fixed on the light source in a threaded mode, and the light source fixing ring is fixed on the lens in a threaded mode. The light source adjusting block is sequentially connected with the light source fixture and the light source fixing ring in a threaded manner; and the light source adjusting module is used for adjusting the angle between the light source and the lens until the camera displays light which is uniformly paved on the whole observation interface and then is fixed. The method is suitable for vaccine industry and other fields.

Description

Technical Field

[0001] The invention relates to the technical field of intelligent control. Background Art

[0002] Double transmission observation has high clarity, strong adaptability, and a large observation area, which improves the accuracy of cell culture results to a certain extent. The transmission light source assists imaging by penetrating the liquid surface in the culture dish into the microscope imaging lens. The brightness and clarity of the image can be adjusted by adjusting the brightness of the light source and the angle between the light source and the microscope imaging lens. Images formed by brightness and angles within a certain range can be compared. The parameters of the light source in each light-sensing environment are determined according to the clarity of the observed image sample.

[0003] The traditional observation method is to move the culture dish in a small range of single transmission for observation, which can only observe limited positions and does not have the function of accurately analyzing the results. In addition, the range of movement of the culture dish is small, only one-fourth of the entire plane at most; only the cell culture status of the bottom layer can be observed; manual operation is required, which is not automated enough, and the stop operation button is required to take pictures; the shooting range is small, and the data obtained is not accurate enough. Summary of the invention

[0004] The present invention aims at the problem that the traditional observation method is to observe by moving the culture dish in a small range of a single transmission, which can only observe a limited position and does not have the function of accurately analyzing the results. To solve the above technical problems, the present invention is implemented by the following technical solutions:

[0005] The present invention proposes a fully automatic dual-lens microscopic detection and observation device, the device comprises a top device and a bottom device, the top device comprises a main control module and an upper motion module;

[0006] The bottom device includes a lower motion module and an optical module;

[0007] The main control module is arranged in front of the device and is used to display the image of the device to be tested;

[0008] The lower motion module is installed on the slide rail at the bottom of the device by means of threads and fixed on the bottom plate of the instrument;

[0009] The optical module is fixed on the lower motion module by means of threads, and the final clear image is obtained by the lower motion module moving along the XYZ axis in three directions;

[0010] The upper motion module is installed on the slide rail at the bottom of the device by means of threads and fixed on the top plate of the instrument;

[0011] The optical module includes a light source, a light source fixture, a camera, a light source adjustment block, a light source fixing ring, and a lens;

[0012] The light source fixture is fixed on the light source by means of threads, and the light source fixing ring is fixed on the lens by means of threads; the light source adjustment block is connected to the light source fixture and the light source fixing ring in sequence by means of threads;

[0013] The light source adjustment module is used to adjust the angle between the light source and the lens until the camera shows that the light is evenly spread over the entire observation interface and then fixed.

[0014] Furthermore, a preferred embodiment is provided, wherein the upper motion module comprises an upper auto-focus module, and the upper auto-focus module is arranged on the upper motion module.

[0015] Furthermore, a preferred embodiment is provided, wherein the device further comprises 6 stepper motors, and the stepper motors are fixed to the respective motor brackets by means of threads.

[0016] Further, a preferred embodiment is provided, wherein the lower motion module comprises a lower autofocus module, a lower lifting block, a lower lifting screw and a lower optical module adapter assembly;

[0017] The lower optical module adapter is fixed to the lower lifting block by means of threads, and the lower lifting screw is fixed to the lower lifting block by means of its own threads.

[0018] Furthermore, a preferred embodiment is provided, wherein the lower autofocus module is fixed on a slider of the module in the x-axis direction of the lower motion module by means of threads.

[0019] Furthermore, a preferred embodiment is provided, wherein a bearing seat is fixed to the upper and lower parts of the lower lifting screw rod, and is used to drive the lower optical module adapter to move up and down to realize the movement of the optical module along the Z-axis direction.

[0020] Furthermore, a preferred embodiment is provided, in which the lower optical module adapter assembly is connected to the optical module via threads.

[0021] Furthermore, a preferred embodiment is provided, in which the camera in the optical module is connected to the microscope lens by means of external threads, and the lower lens fixing ring is fixed to the microscope lens by means of a top screw and a thread; the lower light source fixing ring is fixed to the lower light source adjustment block by means of threads, and then connected to the lower lens fixing ring, and the light source is fixed to the lower light source fixing ring by means of a top screw and a thread, and the image is adjusted by adjusting the upper and lower position of the light source by the tightness of the top screw.

[0022] Furthermore, a preferred embodiment is provided, wherein the device further comprises a tray, and the tray is fixed to a slider of a slide rail on a middle plate of the device by means of threads.

[0023] Solution 2: This solution proposes a fully automatic dual-lens microscopic detection and observation method, which is implemented based on the device described in Solution 1. The method is:

[0024] S1. Place the cell factory culture dish flat on the tray.

[0025] S2, using a stepper motor and a synchronous belt to move the optical module to the initial position in the middle of the cell factory culture dish;

[0026] S3, control the stepper motor through the main control module to drive the lower lifting screw rod to rotate up and down, and drive the lower lifting block and the lower optical module adapter assembly and the optical module in turn.

[0027] If the image to be tested is clear, adjust the light source and the lens angle through the light source adjustment block until the camera shows that the light is evenly spread over the entire observation interface and then fix it;

[0028] If the image to be tested is not clear, the optical module is moved by controlling the stepper motor until a clear image to be tested is determined in the main control module, thus completing the fully automatic dual-lens microscopic detection and observation.

[0029] The present invention is beneficial in that:

[0030] The fully automatic double-lens microscopic detection observation device and method described in the present invention has high definition and high flexibility through double-transmission observation technology, can accurately observe various forms of cells and bacteria and viruses, and reduce the possibility of misjudgment of culture results.

[0031] The fully automatic dual-lens microscopic detection observation device of the present invention reduces the manual operation process, reduces the risk of human error, and improves the reliability of the observation results. At the same time, the automation system speeds up the overall observation process and improves work efficiency.

[0032] The fully automatic dual-lens microscopic detection and observation device described in the present invention is equipped with advanced image processing and data analysis functions, which can quickly observe results and generate detailed reports, making it convenient for on-site observation and analysis personnel to perform specific analysis.

[0033] In summary, the fully automatic double reflection microscopic observation device of the present invention provides a more accurate and convenient solution for cell culture, which helps to improve the error rate of pharmaceutical and vaccine production.

[0034] The present invention is also applicable to the vaccine industry, cell and gene therapy, and stem cell fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic structural diagram of a fully automatic dual-lens microscopic detection and observation device described in Implementation Example 1.

[0036] Figure 2 It is a side view of a fully automatic dual-lens microscopic detection and observation device described in embodiment 1.

[0037] Figure 3 This is a schematic structural diagram of a lower motion module in a fully automatic dual-lens microscopic inspection and observation device described in Implementation Example 1.

[0038] Figure 4 This is a schematic structural diagram of a lower autofocus module in a fully automatic dual-lens microscopic inspection and observation device described in Implementation Example 1.

[0039] Figure 5 This is a schematic diagram of the structure of an optical module in a fully automatic dual-lens microscopic detection and observation device described in Implementation Example 1.

[0040] Figure 6 This is a schematic diagram of the structure of the upper motion module in a fully automatic dual-lens microscopic detection and observation device described in Implementation Example 1.

[0041] Figure 7 This is a flow chart of a fully automatic dual-lens microscopic inspection and observation method described in Implementation Example 9.

[0042] Figure 8 This is a schematic diagram of comparing and analyzing the saved image and the qualified image described in Implementation Method 10.

[0043] In the figure, main control module 1, lower motion module 2, optical module 3, upper motion module 4, tray 5, stepper motor 6, lower autofocus module 7, lower lifting block 8, lower lifting screw 9, lower optical module adapter assembly 10, light source 11, light source fixture 12, camera 13, light source adjustment block 14, light source fixing ring 15, lens 16, and upper autofocus module 17. DETAILED DESCRIPTION

[0044] In order to make the purpose, technical solutions and advantages of the implementation methods of the present application clearer, the technical solutions in the implementation methods of the present application will be clearly and completely described below in conjunction with the drawings in the implementation methods of the present application. Obviously, the described implementation methods are only part of the implementation methods of the present application, not all of the implementation methods.

[0045] Embodiment 1: This embodiment provides a fully automatic dual-lens microscopic detection and observation device, the device includes a top device and a bottom device, the top device includes a main control module 1 and an upper motion module 4;

[0046] The bottom device includes a lower motion module 2 and an optical module 3;

[0047] The main control module 1 is arranged in front of the device and is used to display the image of the device under test;

[0048] The lower motion module 2 is installed on the slide rail at the bottom of the device by means of threads and fixed on the bottom plate of the instrument;

[0049] The optical module 3 is fixed on the lower motion module 2 by means of threads, and the final clear image is obtained by the lower motion module 2 moving along the three directions of the XYZ axis;

[0050] The upper movement module 4 is installed on the slide rail at the bottom of the device by means of threads and fixed on the top plate of the device;

[0051] The optical module 3 includes a light source 11, a light source fixture 12, a camera 13, a light source adjustment block 14, a light source fixing ring 15, and a lens 16;

[0052] The light source fixture 12 is fixed to the light source 11 by means of threads, and the light source fixing ring 15 is fixed to the lens 16 by means of threads; the light source adjustment block 14 is connected to the light source fixture 12 and the light source fixing ring 15 in sequence by means of threads;

[0053] The light source adjustment module 14 is used to adjust the angle between the light source 11 and the lens 16 until the camera 13 shows that the light is evenly spread over the entire observation interface and then fixed.

[0054] Embodiment 2: This embodiment further limits the fully automatic dual-lens microscopic detection and observation device described in Embodiment 1. The upper motion module 4 includes an upper auto-focus module 17 , and the upper auto-focus module 17 is disposed on the upper motion module 4 .

[0055] Embodiment 3: This embodiment further limits the fully automatic dual-lens microscopic detection and observation device described in embodiment 1. The device also includes 6 stepper motors 6, and the stepper motors 6 are fixed to each motor bracket by means of threads.

[0056] Embodiment 4: This embodiment is a further limitation of the fully automatic dual-lens microscopic inspection and observation device described in Embodiment 1, wherein the lower motion module 2 includes a lower autofocus module 7, a lower lifting block 8, a lower lifting screw 9 and a lower optical module adapter assembly 10;

[0057] The lower optical module adapter 10 is fixed to the lower lifting block 8 by means of threads, and the lower lifting screw rod 9 is fixed to the lower lifting block 8 by means of its own threads.

[0058] Embodiment 5: This embodiment further limits the fully automatic dual-lens microscopic inspection and observation device described in Embodiment 4. The lower autofocus module 7 is fixed on the slider of the module in the x-axis direction of the lower motion module 2 by means of threads.

[0059] Embodiment 6. This embodiment is a further limitation of the fully automatic dual-lens microscopic inspection and observation device described in embodiment 4. The lower lifting screw 9 is fixed with a bearing seat above and below, which is used to drive the lower optical module adapter 10 to move up and down to realize the movement of the optical module along the Z-axis direction.

[0060] Embodiment 7: This embodiment further limits the fully automatic dual-lens microscopic inspection and observation device described in Embodiment 5. The lower optical module adapter assembly 10 is connected to the optical module 3 via threads.

[0061] Embodiment 8. This embodiment is a further limitation of the fully automatic dual-lens microscopic detection and observation device described in embodiment 1. The camera 13 in the optical module 3 is connected to the microscope lens 16 by an external thread, and the lower lens fixing ring 15 is fixed to the microscope lens 16 by a top screw and a thread. The lower light source fixing ring 12 is fixed to the lower light source adjustment block 14 by a thread, and then connected to the lower lens fixing ring 15. The light source 11 is fixed to the lower light source fixing ring 12 by a top screw and a thread. The image is adjusted by adjusting the upper and lower positions of the light source 11 by adjusting the tightness of the top screw.

[0062] Embodiment 9. This embodiment is a further limitation of the fully automatic dual-lens microscopic inspection and observation device described in Embodiment 1. The device further includes a tray 5, which is fixed to the slider of the slide rail on the middle plate of the device by threads.

[0063] Embodiment 10: This embodiment proposes a fully automatic dual-lens microscopic detection and observation method, which is implemented based on the device described in the first embodiment. The method is:

[0064] S1. Place the cell factory culture dish flatly in tray 5;

[0065] S2, using the stepper motor 6 to move the optical module 3 to the initial position in the middle of the cell factory culture dish through a synchronous belt;

[0066] S3, control the stepper motor 6 through the main control module 1 to drive the lower lifting screw 9 to rotate up and down, and drive the lower lifting block 8, the lower optical module adapter assembly 10 and the optical module 3 in turn,

[0067] If the image to be measured is clear, the light source 11 and the lens 16 angles are adjusted by the light source adjustment block 14 until the camera 13 shows that the light is evenly spread over the entire observation interface and then fixed;

[0068] If the image to be tested is not clear, the optical module 3 is moved by controlling the stepping motor 6 until a clear image to be tested is determined in the main control module 1, thus completing the fully automatic dual-lens microscopic detection and observation.

[0069] Implementation eleven: This implementation provides an example, which is used to explain the above implementations one to nine. The specific example is as follows:

[0070] See also Figure 1 and Figure 6 To explain this embodiment, Figure 1 In the process, the main control center 1 is a process of pressing the inner wall of the instrument shell with four screws and its own threads through the locking nut. The lower motion module 2 fixes the bottom rail of the entire module to the instrument bottom plate through threads. The optical module 3 is fixed to the lower motion module 2 by threads, and the final clear image is obtained through the three-axis movement of the 2 lower motion modules XYZ.

[0071] Figure 2 In the figure, the upper motion module 4 fixes the bottom rail of the entire module to the top plate of the instrument by means of threads, and a clear image is obtained through XYZ three-axis motion. The tray 5 is fixed to the slider of the rail on the middle plate of the instrument by means of threads, and is used to place the parts of the cell culture dish, and the culture dish can be easily placed by sliding the rail back and forth.

[0072] Figure 3 In the device, there are 6 stepper motors 6 fixed on each motor bracket by threads. The lower autofocus module 7 is fixed on the slider of the module in the x-axis direction of the lower motion module 2 by threads. The optical system can be focused by moving along the Z axis.

[0073] Figure 4 In the figure, the lower optical module adapter 10 is fixed to the lower lifting block 8 by means of threads, and the lower lifting screw rod 9 is fixed to the lower lifting block 8 by means of its own threads, and a bearing seat is fixed above and below, which drives the lower optical module adapter 10 to move up and down by its own rotation, so as to realize the movement of the optical module along the Z-axis direction, and can adjust the focal length of the optical system to obtain a clear image.

[0074] Figure 5In the figure, the camera 13 is connected to the microscope lens 16 by external thread, and the lower lens fixing ring 15 is fixed to the microscope lens 16 by means of a top screw with a thread, and the position can be adjusted up and down by loosening the top screw to adjust the optical effect, which plays an important role in clear image acquisition. The lower light source fixing ring 12 is fixed to the lower light source adjustment block 14 by a thread, and is connected to the lower lens fixing ring 15 in the same way, and the screws on both sides of the lower light source adjustment block 14 can adjust the angle between the light source and the lens by loosening and tightening to adjust the image clarity. The light source 11 is fixed to the lower light source fixing ring 12 by means of a top screw and a thread, and the image can also be adjusted by adjusting the position of the light source 11 up and down by the looseness of the top screw. All the screws of this module can be adjusted to adjust the image clarity by adjusting the tightness. It is very important to obtain a clear image.

[0075] Figure 6 In the figure, the upper auto-focus module 17 is fixed on the support plate of the upper motion module 4 in the X-axis motion direction by means of threads, and a clear image can be quickly obtained by the auto-focusing function.

[0076] This embodiment is first based on a fully automatic dual-lens microscopic inspection and observation device described in Implementation Method 1, which is equipped with a mirrored stainless steel closed shell, which is used to prevent the clean workshop from spraying corrosive gases or liquids during disinfection, and can effectively prevent corrosion. The automated operation is simple, reduces labor costs, reduces human errors and improves efficiency.

[0077] Secondly, the device described in this embodiment is also provided with a high-magnification lens, which can observe the entire growth state of the cells. The data and pictures are more simple and clear. And through modular docking, the structure is simple and easy to repair and maintain.

[0078] Save the image for archiving. You can compare the saved image with the standard image and data later to determine the final result.

[0079] The production application range is wide and it is suitable for many fields, including hospitals, research institutes, laboratories, vaccine companies, etc. Purpose: To promote and apply it widely, improve the overall cell gene culture level, and improve the progress of biomedical engineering.

[0080] It also provides high-precision, multi-magnification cell culture image data to provide reliable data support for production personnel. Purpose: To promote the development of allergen-related research and explore new allergy mechanisms and treatment methods. To promote the development of vaccine industry and stem cell industries and explore new biomedical processes. By designing a cell factory microscopic observation device, it aims to achieve more efficient, accurate and comprehensive cell culture observation, meet the needs of vaccine production and scientific research, and improve the development of biomedicine.

[0081] Those skilled in the art will appreciate that the above are only preferred embodiments of the present invention, and the various embodiments of the present disclosure and / or the features described in the claims may be combined or combined in various ways, even if such combinations or combinations are not explicitly described in the present disclosure. It is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or perform equivalent substitutions on some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

[0082] Although preferred embodiments of the present invention have been described, additional changes and modifications may be made to these embodiments by those skilled in the art once the basic inventive concepts are known. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention. Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A fully automatic dual-lens microscopic detection and observation device, characterized in that: The device comprises a top device and a bottom device, wherein the top device comprises a main control module (1) and an upper movement module (4); The bottom device comprises a lower motion module (2) and an optical module (3); The main control module (1) is arranged in front of the device and is used to display an image of the device to be tested; The lower motion module (2) is installed on the slide rail at the bottom of the device by means of threads and fixed on the bottom plate of the instrument; The optical module (3) is fixed on the lower motion module (2) by means of threads, and a final clear image is obtained by the lower motion module (2) moving along the XYZ axes in three directions; The upper movement module (4) is installed on the slide rail at the bottom of the device by means of threads and fixed on the top plate of the instrument; The optical module (3) comprises a light source (11), a light source fixture (12), a camera (13), a light source adjustment block (14), a light source fixing ring (15), and a lens (16); The light source fixture (12) is fixed to the light source (11) by means of threads, and the light source fixing ring (15) is fixed to the lens (16) by means of threads; the light source adjustment block (14) is connected to the light source fixture (12) and the light source fixing ring (15) in sequence by means of threads; The light source adjustment module (14) is used to adjust the angle between the light source (11) and the lens (16) until the camera (13) displays that the light is evenly spread over the entire observation interface and then fixed.

2. The fully automatic dual-lens microscopic inspection and observation device according to claim 1, characterized in that: The upper movement module (4) comprises an upper automatic focusing module (17), and the upper automatic focusing module (17) is arranged on the upper movement module (4).

3. The fully automatic dual-lens microscopic inspection and observation device according to claim 1, characterized in that: The device also includes six stepper motors (6), and the stepper motors (6) are fixed on each motor bracket by means of threads.

4. The fully automatic dual-lens microscopic inspection and observation device according to claim 1, characterized in that: The lower motion module (2) comprises a lower autofocus module (7), a lower lifting block (8), a lower lifting screw (9) and a lower optical module adapter assembly (10); The lower optical module adapter (10) is fixed to the lower lifting block (8) by means of threads, and the lower lifting screw rod (9) is fixed to the lower lifting block (8) by means of its own threads.

5. The fully automatic dual-lens microscopic inspection and observation device according to claim 4, characterized in that: The lower automatic focusing module (7) is fixed on a slider of the module in the x-axis direction of the lower motion module (2) by means of threads.

6. The fully automatic dual-lens microscopic inspection and observation device according to claim 4, characterized in that: The lower lifting screw rod (9) is fixed with a bearing seat at the top and bottom, which is used to drive the lower optical module adapter (10) to move up and down to realize the movement of the optical module along the Z-axis direction.

7. The fully automatic dual-lens microscopic inspection and observation device according to claim 5, characterized in that: The lower optical module adapter assembly (10) is connected to the optical module (3) via threads.

8. The fully automatic dual-lens microscopic inspection and observation device according to claim 1, characterized in that: The camera (13) in the optical module (3) is connected to the microscope lens (16) by means of external threads, and the lower lens fixing ring (15) is fixed to the microscope lens (16) by means of a top screw and a thread; the lower light source fixing ring (12) is fixed to the lower light source adjustment block (14) by means of threads, and then connected to the lower lens fixing ring (15); the light source (11) is fixed to the lower light source fixing ring (12) by means of a top screw and a thread, and the image is adjusted by adjusting the upper and lower positions of the light source (11) by the tightness of the top screw.

9. The fully automatic dual-lens microscopic inspection and observation device according to claim 1, characterized in that: The device also comprises a tray (5), which is fixed on a slide block of a slide rail on the middle plate of the device via threads.

10. A fully automatic dual-lens microscopic detection and observation method, characterized in that: The method is implemented based on the device according to claim 1, and the method is: S1. Place the cell factory culture dish flatly in the tray (5); S2, using a stepper motor (6) to move the optical module (3) to an initial position in the middle of the cell factory culture dish through a synchronous belt; S3, controlling the stepper motor (6) through the main control module (1) to drive the lower lifting screw rod (9) to rotate up and down, and in turn drive the lower lifting block (8) and the lower optical module adapter assembly (10) and the optical module (3), If the image to be measured is clear, the light source (11) and the lens (16) are adjusted by the light source adjustment block (14) until the camera (13) shows that the light is evenly spread over the entire observation interface and then fixed; If the image to be tested is not clear, the optical module (3) is moved by controlling the stepping motor (6) until a clear image to be tested is determined in the main control module (1), thus completing the fully automatic dual-lens microscopic detection and observation.

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