Preparation and fermentation method of composition for palm meal functional solid fermentation material

Abstract

The invention belongs to the field of additives, and discloses a composition for a palm meal functional solid-state fermentation material, which comprises palm meal, wheat bran, a nutrient solution, a concentrated fish cream hydrolysate, soybean molasses, a composite strain seed solution, a barley pest larva intestinal culture and a hermetia illucens intestinal culture. The composite strain in the composite strain seed solution is prepared from bacillus, saccharomycetes, koji bacteria and lactobacillus plantarum. According to the composition, the compound bacteria are adopted for anaerobic fermentation, meanwhile, the activity of the strains is improved by concentrating the fish pulp hydrolysate and the soybean molasses, nutritional support is provided for efficient exertion of the strains, and the composition can effectively reduce the content of crude fibers and mannan in the palm meal and effectively decompose the crude fibers and the mannan. The invention also aims to provide a fermentation method of the palm meal.

Description

Technical Field

[0001] The invention belongs to the biological field, and particularly relates to a composition preparation and fermentation method for palm meal functional solid-state fermentation material. Background Art

[0002] Palm meal is a byproduct of palm kernel shelling and oil extraction, with a crude protein content of 14%-21%, a crude fiber content of 21%-23%, and a crude fat content of 8%-17%. Palm meal is directly fed to animals, but due to its high fiber content, poor palatability, low energy and protein utilization, and extremely low addition ratio in feed (such as 1%-7% for poultry and 3%-9% for pigs), the cheap palm meal cannot be effectively used in the feed industry.

[0003] CN111642618A discloses a ruminant fermented feed and a preparation method thereof, belonging to the field of feed technology. The raw materials used in the feed include the following components in parts by weight: 22-30 parts of corn, 2-10 parts of sprayed corn husks, 4-12 parts of DDGS, 6-14 parts of corn germ meal, 10-20 parts of wheat bran, 1.5-5.5 parts of cottonseed meal, 2-8 parts of palm meal, 2-8 parts of sesame meal, 1.2-3.2 parts of glutamic acid residue, 0.5-1.5 parts of molasses, 0.05-0.15 parts of live bacteria fermentation agent, 0.05-0.15 parts of complex enzyme preparation, and 18-26 parts of water. The live bacteria fermentation agent includes brewer's yeast, lactic acid bacteria and bacillus, and the weight ratio of brewer's yeast, lactic acid bacteria and bacillus is (2.5-3.5): (3.8-4.2): (1.7-2.3).

[0004] CN110463828A discloses a method for producing biological feed from palm meal. The method uses palm meal as raw material and adopts a combination of enzymatic hydrolysis and microbial fermentation to obtain a palm meal fermented feed with good palatability, high energy and protein utilization, and rich in probiotics, small molecule peptides, lactic acid, biological enzymes, oligosaccharides, etc. The palm meal fermented feed obtained by the method has a pH of 4.2-5.0, NDF of 33%-40%, a small molecule peptide content of 27%-33%, an amino acid nitrogen content of 1.89-2.10%, a crude protein content of 13.5-17.0%, which is slightly higher than that of the starting raw material, and a lactic acid colony count of 5.1-7.0×10 8 cfu / g, the number of Bacillus subtilis spores is 6.2-8×10 9 cfu / g, protease activity 120-210u / g, saccharifying enzyme activity 310-620u / g, mannanase activity 1.5-2.8u / g, adding a certain amount of vitamins, trace elements, lysine and methionine, can replace 20%-40% (1:1 equal replacement) of complete animal diet.

[0005] It can be seen that fermentation is a common method to reduce the fiber content of palm meal and increase the protein content.

[0006] Palm meal has a certain application value due to its rich nutritional components. Many scholars at home and abroad have reported that palm meal is more suitable for ruminant diets, but there are also relevant reports on the application of palm meal in pigs, rabbits, poultry and aquatic animals, which have determined that palm meal should be used in limited amounts in diets, such as pig feed should not exceed 20%, chicken feed should not exceed 10% and aquatic feed should not exceed 30%. Due to the high crude fiber content, high manno-oligosaccharide content, and low energy and crude protein utilization, palm meal is not suitable for young and growing animals, and excessive feeding of some monogastric animals will also affect their growth performance, which limits the widespread use of palm meal.

[0007] However, for palm meal, there are several components that need to be controlled in dosage, such as crude fat and mannan. Taking mannan as an example, its content is as high as 360 mg / g; mannan has low heat, stability, safety and non-toxic physical and chemical properties, and also has the functions of protecting the intestine and improving immunity. Many scholars at home and abroad have reported that palm meal is more suitable for ruminant diets, but there are also relevant reports on the application research in pigs, rabbits, poultry and aquatic animals, which have determined that palm meal is used in limited amounts in diets, such as pig feed should not exceed 20%, chicken feed should not exceed 10% and aquatic feed should not exceed 30%. Due to high crude fiber content, high manno-oligosaccharide content, and low energy and crude protein utilization, palm meal is not suitable for young and growing animals, and excessive feeding of some monogastric animals will also affect their growth performance, which limits the widespread use of palm meal. Therefore, controlling the content of crude fiber and mannan in palm meal is a key point of this application.

[0008] Therefore, the technical problem to be solved in this case is: how to increase the crude protein content of palm meal and reduce the fiber, fat and mannan content of palm meal. Summary of the invention

[0009] In view of the shortcomings and deficiencies of the current prior art, the primary purpose of the present invention is to provide a composition for palm meal functional solid fermentation material, which uses composite bacteria for anaerobic fermentation. At the same time, the activity of the bacteria is improved by concentrating fish paste hydrolysate and soybean molasses, providing nutritional support for the bacteria to efficiently exert their efficacy. The composition of the present invention can effectively reduce the fiber, fat and mannan content of palm meal.

[0010] Another object of the present invention is to provide a fermentation method of the palm meal.

[0011] The specific plan is as follows:

[0012] A composition for palm meal functional solid fermentation material, comprising palm meal, wheat bran, nutrient solution, concentrated fish paste hydrolyzate, soybean molasses, composite bacterial seed liquid, barley worm larvae intestinal culture, and black soldier fly intestinal culture;

[0013] The composite bacterial species in the composite bacterial species seed liquid are composed of bacillus, yeast, aspergillus and lactobacillus plantarum;

[0014] Each 100kg composition contains 3-5kg of composite bacterial seed liquid; the concentration of each bacterium in the composite bacterial seed liquid is Bacillus content: 1-1.5×10 10 cfu / ml, yeast content: 3.5-5.0×10 9 cfu / ml, Aspergillus content: 3.5-5.5×10 9 cfu / ml, Lactobacillus plantarum content: 5.0-10×10 8 cfu / ml.

[0015] After the above treatment, the technical parameters of the composition are:

[0016] Morphology: Electron microscopy shows that the structure is loose and porous, the fibers are evenly broken, and the sensory quality is significantly improved;

[0017] Color: dark coffee or dark brown with special aromas such as wine and coffee.

[0018] The crude protein content shall not be less than 20.0%;

[0019] Crude fat content 2.5-3.5%;

[0020] Crude fiber should not exceed 10.0%;

[0021] The crude ash content shall not exceed 5.0%;

[0022] Mannan (mg / g) shall not exceed 100mg / g;

[0023] Aflatoxin should comply with: GB 31656-2021;

[0024] Escherichia coli should comply with: GB 4789.3-2016;

[0025] Salmonella should comply with: GB 4789.4-2016.

[0026] In the above-mentioned composition for palm meal functional solid-state fermentation material, the weight ratio of palm meal, wheat bran, nutrient solution, concentrated fish paste hydrolyzate, soybean molasses, composite bacterial seed liquid, mealworm larvae intestinal culture, and black soldier fly intestinal culture is: 55-65:12-17:1:4-6:12-17:3-5:1-5:1-5.

[0027] In the above-mentioned composition for palm meal functional solid-state fermentation material, the weight ratio of palm meal, wheat bran, nutrient solution, concentrated fish paste hydrolyzate, soybean molasses, composite bacterial seed liquid, mealworm larvae intestinal culture, and black soldier fly intestinal culture is: 60:15:1:5:15:4:3:3.

[0028] In the above-mentioned composition for palm meal functional solid fermentation feed, the ingredients of the nutrient solution are: 4.0wt% (NH 4 ) 2 SO 4 , 0.4wt% MgSO 4 7H 2 O, 0.2wt% KH 2 PO 4 , and the balance water.

[0029] In the above-mentioned composition for palm meal functional solid-state fermentation feed, the solid content of the concentrated fish paste hydrolyzate is 35%.

[0030] In the above-mentioned composition for palm meal functional solid-state fermentation material, the preparation method of the concentrated fish paste enzymatic hydrolysis product is: after the fish paste is concentrated, it is transferred into an enzymatic hydrolysis tank, and Bacillus subtilis, actinomycetes, Aspergillus niger fermentation products and Aspergillus oryzae fermentation products are added, stirred evenly, and bacterial hydrolysis and enzymatic hydrolysis are carried out at room temperature (≧20°C), and the concentrated fish paste enzymatic hydrolysis product can be obtained by synergistic treatment.

[0031] In the above-mentioned composition for palm meal functional solid-state fermentation material, during the fermentation, a proper amount of water is added to adjust the moisture content of the composition to 38%.

[0032] In the above-mentioned composition for palm meal functional solid-state fermentation feed, the preparation method of the barleyworm larvae intestinal culture and the black soldier fly intestinal culture is:

[0033] The intestinal tract of the corresponding insect body is homogenized to obtain a slurry, which is inoculated into a mixed liquid culture medium at an inoculation rate of 3 to 5 wt%, anaerobically cultured for 36 to 48 hours, and freeze-dried to obtain a slurry having a solid content of 45% to 65%;

[0034] The mixed liquid culture medium contains the following components in a weight ratio: NA:TSA:LB:MRS=4:2:2:2.

[0035] At the same time, the present invention also discloses a palm meal fermentation method, which uses any of the above compositions, adjusts the moisture content of the composition to 35% to 45%, places it in an anaerobic fermentation bag for sealed fermentation, and has a temperature of 25°C to 35°C, a fermentation time of 3 to 5 days, a gas release number of 3 to 5 times, and a material pH value of 5.5 to 6.5.

[0036] In the above palm meal fermentation method, the palm meal has a mesh size of 40-60.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] The composite bacterial strains of the invention contain bacillus, yeast, aspergillus and lactobacillus plantarum; the bacillus has the function of producing protease and; the yeast and aspergillus have the function of producing cellulase; the metabolites of lactobacillus plantarum are organic acid and fatty acid; the bacterial strains contained in the intestinal culture of barley worm larvae have the function of producing mannanase, and the bacterial strains contained in the intestinal culture of black soldier flies have the function of producing lipase. The above bacteria and cultures work together to reduce fat content and improve the efficiency of converting cellulose and mannan into protein.

[0039] The composition of the invention adopts concentrated fish paste hydrolyzate and soybean molasses to provide nutrients for bacteria in the culture medium, thereby improving the activity of the bacteria. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 Photograph of the culture medium activated for the gut culture of the mealworm larvae;

[0041] Figure 2 This is a photo of the transparent circle showing the cellulase production capacity of the intestinal culture of the barleyworm larvae;

[0042] Figure 3 Photo of the culture medium activated for the black soldier fly gut culture;

[0043] Figure 4 This is a photo of the transparent circle showing the ability of the intestinal culture of black soldier flies to produce lipase;

[0044] Figure 5 are microscopic photographs before and after fermentation, wherein UFO represents a microscopic photograph of palm meal before fermentation (2 photos, the same sample, different sampling), and FO represents a microscopic photograph of palm meal after fermentation of Example 1 (2 photos, the same sample, different sampling);

[0045] Figure 6 These are electron microscope scans of palm meal cork cells before and after fermentation (2 images, the same sample, different sampling, the left is before fermentation; the right is after fermentation);

[0046] Figure 7These are electron microscope scans of palm meal wood fiber before and after fermentation (2 images, same sample, different sampling, the left one is before fermentation; the right one is after fermentation). DETAILED DESCRIPTION

[0047] The present invention is further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto.

[0048] Before describing the embodiments and comparative examples of the present invention, the detailed preparation process of the intestinal cultures of barleyworm larvae, the intestinal cultures of black soldier flies, the concentrated fish paste hydrolyzate and the raw materials is introduced:

[0049] Preparation of intestinal cultures of barleyworm larvae (reference CN113493288A, a composition for fermentation and deodorization of livestock and poultry manure and its preparation method):

[0050] Under aseptic operation conditions, take the intestinal homogenate of the mealworm larvae, add it into a fermenter containing 50L mixed liquid culture medium I (NA:TSA:LB:MRS=4:2:2:2) at a weight of 4wt%, and culture it anaerobically for 36-48 hours. After the fermentation is completed, take out the culture solution and freeze-dry it in a vacuum for 6-8 hours to obtain the intestinal cavity and intestinal culture of the mealworm larvae. According to the plate colony count: the total bacterial count of the intestinal culture of the mealworm larvae is ≥4.5×10 10 cfu / g. The enzyme production capacity was qualitatively evaluated by Congo red medium, and the maximum transparent zone diameter was ≥40.24mm.

[0051] Figure 1 Photograph of the culture medium activated for the gut culture of the mealworm larvae;

[0052] Figure 2 This is a photo of the transparent circle showing the cellulase production capacity of the intestinal culture of the mealworm larvae.

[0053] Preparation of black soldier fly intestinal culture (reference CN113493288A a composition for fermentation and deodorization of livestock and poultry manure and its preparation method):

[0054] Under aseptic operation conditions, the intestinal homogenate of black soldier fly larvae was taken and added to a fermenter containing 50L mixed liquid culture medium I (NA:TSA:LB:MRS=4:2:2:2) at a weight of 4wt%, and anaerobically cultured for 36-48 hours. After the fermentation, the culture solution was taken out and vacuum-freeze-dried for 6-8 hours to obtain the intestinal cavity and intestinal culture of the mealworm larvae. The total bacterial count of the intestinal culture of the mealworm larvae was ≥3.5×10 10 cfu / g. The enzyme production capacity was qualitatively evaluated by WTL medium, and the maximum transparent zone diameter was ≥41.18 mm.

[0055] Enzyme production capacity assessment medium (WTL): 2.5 ml of olive oil, 1.0 g of peptone, 0.5 g of beef extract, 0.3 g of glucose, 1.0 g of PVA, 0.5 g of sodium chloride, 0.5 ml of Tween 80, 1.5 g of agar, 0.016% bromocresol purple, distilled water to 100 ml, adjusted to pH 7.5, and sterilized at 121°C under high pressure for 15 to 20 min for use.

[0056] Figure 3 Photo of the culture medium activated for the black soldier fly gut culture;

[0057] Figure 4 This is a photo of the transparent circle showing the ability of the intestinal culture of black soldier flies to produce lipase;

[0058] Preparation of concentrated fish paste hydrolysate: After the fish paste (produced by Guangdong Yuechuang Ecological Agriculture Technology Co., Ltd.) is concentrated (dry matter content is about 60%), 1000g of the concentrated fish paste is moved into an enzymolysis tank, 5g of Bacillus subtilis (10 billion / g), 2.5g of actinomycetes (10 billion / g), 10g of Aspergillus niger fermentation product and 10g of Aspergillus oryzae fermentation product are added, stirred evenly, and bacterial hydrolysis and enzymolysis are carried out at room temperature (≧20°C), and the concentrated fish paste enzymolysis product is obtained by coordinated treatment.

[0059] The composition of the nutrient solution is: 4.0wt% (NH 4 ) 2 SO 4 , 0.4wt% MgSO 4 7H 2 O, 0.2wt% KH 2 PO 4 , and the balance water.

[0060] Bacillus subtilis, actinomycetes, Aspergillus niger fermentation products, Aspergillus oryzae fermentation products, yeast, Aspergillus niger, Lactobacillus plantarum, and commercially available complex enzymes are all from Shandong Hezhong Kangyuan Biotechnology Co., Ltd.

[0061] Examples 1-7

[0062] The palm meal is controlled to be 40-60 mesh, the moisture content of the composition is adjusted to 40%, and the mixture is placed in an anaerobic fermentation bag for sealed fermentation. The fermentation is carried out at room temperature, the temperature is 25° C. to 35° C., the fermentation time is 5 days, the number of deflation is three times (deflation is performed once every 18-24 hours), and the pH value of the material is 5.5 to 6.5.

[0063] The dosage of each component of the composition is shown in Table 1

[0064] Table 1

[0065]

[0066] The concentration of each bacteria in the composite bacterial seed liquid is Bacillus content: 1.2×10 10 cfu / ml, yeast content: 4×10 9 cfu / ml, Aspergillus content: 4×10 9 cfu / ml, Lactobacillus plantarum content: 8×10 8 cfu / ml.

[0067] Comparative Examples 1-3

[0068] The palm meal is controlled to be 40-60 mesh, the moisture content of the composition is adjusted to 40%, and the composition is placed in an anaerobic fermentation bag for sealed fermentation. The fermentation is carried out at room temperature, the temperature is 25° C. to 35° C., the fermentation time is 5 days, the number of deflations is 5 times (deflation every day), and the pH value of the material is 5.5 to 6.5.

[0069] The dosage of each component of the composition is shown in Table 2;

[0070] Table 2

[0071]

[0072]

[0073] The concentration of each bacteria in the composite bacterial seed liquid is Bacillus content: 1.2×10 10 cfu / ml, yeast content: 4×10 9 cfu / ml, Aspergillus content: 4×10 9 cfu / ml, Lactobacillus plantarum content: 8×10 8 cfu / ml.

[0074] Comparative Example 4

[0075] The same as Example 1, except that the composite bacterial seed solution only contains yeast, Aspergillus, and Lactobacillus plantarum; the concentration of each bacteria is: yeast content: 4×10 9 cfu / ml, Aspergillus content: 8×10 9 cfu / ml, Lactobacillus plantarum content: 8×10 8 cfu / ml.

[0076] Comparative Example 5

[0077] The same as Example 1, except that the composite bacterial seed solution only contains Bacillus, yeast, and Lactobacillus plantarum; the concentration of each bacterium in the composite bacterial seed solution is: Bacillus content: 1.2×10 10 cfu / ml, yeast content: 8×10 9 cfu / ml, Lactobacillus plantarum content: 8×108 cfu / ml.

[0078] Comparative Example 6

[0079] The same as Example 1, except that the composite bacterial seed solution only contains Bacillus, yeast, and Aspergillus; the concentration of each bacteria in the composite bacterial seed solution is: Bacillus content: 1.2×10 10 cfu / ml, yeast content: 4.5×10 9 cfu / ml, Aspergillus content: 4.5×10 9 cfu / ml.

[0080] Comparative Example 7

[0081] The method is substantially the same as Example 1, except that: the preparation method of the concentrated fish paste enzymatic hydrolysis product is as follows: after the fish paste is concentrated, it is transferred into an enzymatic hydrolysis tank, a commercially available complex enzyme is added, stirred evenly, and enzymatic hydrolysis is performed at 50-60° C. for a certain period of time, a preservative is added, and stirred evenly to obtain a concentrated fish paste enzymatic hydrolysis product.

[0082] Performance Testing

[0083] The fermentation products of Examples 1-7 and Comparative Examples 1-7 were tested for content. Note: “—” indicates the lower limit of detection.

[0084] The specific results can be seen in Tables 3 and 4;

[0085] Figure 5 Microscopic photographs before and after fermentation are shown, wherein UFO represents the microscopic photograph of palm meal before fermentation (2 photos, same sample, different sampling), and FO represents the microscopic photograph of palm meal after fermentation of Example 1 (2 photos, same sample, different sampling). Figure 6 and Figure 7 These are electron microscope scans of palm meal before and after fermentation (2 images, same sample, different sampling).

[0086] Table 3 Test results of substance content of fermentation products of Examples 1-7

[0087]

[0088]

[0089] Table 4 Test results of substance content of fermentation products of Comparative Examples 1-7

[0090]

[0091]

[0092] Result analysis:

[0093] 1. By comparing Examples 1-7 with Comparative Examples 1-7, it can be seen that the Examples are generally better than the Comparative Examples, indicating that the concentrated fish paste hydrolyzate, the composite bacterial seed liquid, the barley worm larvae intestinal culture, and the black soldier fly intestinal culture are all very important for the final form and nutrient content of palm meal, and none of them can be missing;

[0094] 2. It can be seen from Examples 1-7 that among the concentrated fish paste hydrolysate, the composite bacterial seed liquid, the intestinal culture of barley worm larvae, and the intestinal culture of black soldier flies, according to the performance change trend, the composite bacterial seed liquid and the intestinal culture of black soldier flies are more important, and the other two are second;

[0095] 3. Comparative Examples 1-3 show that the composite bacterial seed solution, the intestinal cultures of barleyworm larvae, and the intestinal cultures of black soldier flies are indispensable;

[0096] 4. Comparative Examples 4-6 show that yeast, Aspergillus niger, and Lactobacillus plantarum are indispensable in the present invention;

[0097] 5. It can be seen from Comparative Example 7 that the enzyme used in the concentrated fish paste enzymatic hydrolysis product and whether the bacterial hydrolysis and enzymatic hydrolysis are carried out simultaneously are also crucial to the product performance.

[0098] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A composition for palm meal functional solid-state fermentation material, characterized in that: Including palm meal, wheat bran, nutrient solution, concentrated fish paste hydrolyzate, soy molasses, composite bacterial seed liquid, mealworm larvae intestinal culture, black soldier fly intestinal culture; The composite bacterial species in the composite bacterial species seed liquid are composed of bacillus, yeast, aspergillus and lactobacillus plantarum; Each 100kg composition contains 3-5kg of composite bacterial seed liquid; the concentration of each bacterium in the composite bacterial seed liquid is Bacillus content: 1-1.5×10 10 cfu / ml, yeast content: 3.5-5.0×10 9 cfu / ml, Aspergillus content: 3.5-5.5×10 9 cfu / ml, Lactobacillus plantarum content: 5.0-10×10 8 cfu / ml.

2. The composition for palm meal functional solid-state fermentation material according to claim 1, characterized in that: The weight ratio of the palm meal, wheat bran, nutrient solution, concentrated fish paste hydrolyzate, soybean molasses, composite bacterial seed solution, barleyworm larvae intestinal culture, and black soldier fly intestinal culture is: 55-65: 12-17: 1: 4-6: 12-17: 3-5: 1-5: 1-5.

3. The composition for palm meal functional solid-state fermentation material according to claim 2, characterized in that: The weight ratio of the palm meal, wheat bran, nutrient solution, concentrated fish paste hydrolyzate, soybean molasses, composite bacterial seed solution, barleyworm larvae intestinal culture, and black soldier fly intestinal culture is 60:15:1:5:15:4:3:

3.

4. The composition for palm meal functional solid-state fermentation material according to claim 1, characterized in that: The ingredients of the nutrient solution are: 4.0wt% (NH4)2SO4, 0.4wt% MgSO4·7H2O, 0.2wt% KH2PO4, and the balance water.

5. The composition for palm meal functional solid-state fermentation material according to claim 1, characterized in that: The solid content of the concentrated fish paste hydrolysate is 35%.

6. The composition for palm meal functional solid-state fermentation material according to claim 5, characterized in that: The preparation method of the concentrated fish paste hydrolysate is as follows: After the fish paste is concentrated, it is transferred into an enzymolysis tank, and Bacillus subtilis, actinomycetes, fermentation products of Aspergillus niger and fermentation products of Aspergillus oryzae are added, stirred evenly, and subjected to bacterial and enzymatic hydrolysis at room temperature to obtain concentrated fish paste enzymatic hydrolysis products.

7. The composition for palm meal functional solid-state fermentation material according to claim 1, characterized in that: When the composition is fermented, an appropriate amount of water is added to adjust the moisture content of the composition to 38%.

8. The composition for palm meal functional solid-state fermentation material according to claim 1, characterized in that: The preparation method of the intestinal cultures of barleyworm larvae and black soldier fly intestinal cultures is as follows: The intestinal tract of the corresponding insect body is homogenized to obtain a slurry, which is inoculated into a mixed liquid culture medium at an inoculation rate of 3 to 5 wt%, anaerobically cultured for 36 to 48 hours, and freeze-dried to obtain a slurry having a solid content of 45% to 65%; The mixed liquid culture medium contains the following components in a weight ratio: NA:TSA:LB:MRS=4:2:2:

2.

9. A palm meal fermentation method, characterized in that: The composition as claimed in any one of claims 1 to 8 is used, the moisture content of the composition is adjusted to 35% to 45%, and the composition is placed in an anaerobic fermentation bag and sealed for fermentation at a temperature of 25° C. to 35° C., a fermentation time of 3 to 5 days, a gas release number of 3 to 5 times, and a material pH value of 5.5 to 6.

5.

10. The palm meal fermentation method according to claim 9, characterized in that: The palm meal has a mesh size of 40-60.

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