A kind of microbial fertilizer and its application

Abstract

The invention discloses a microbial fertilizer and application thereof, wherein the microbial fertilizer comprises components: Penicillium purpurogenum ( Penicillium yellow flower )、Bacillus mohaiwei( Bacillus Mojave ), biochar, peat soil, corn stalk powder, bentonite and γ-polyglutamic acid. The microbial fertilizer provided by the present invention can effectively prevent and control Fusarium oxysporum ( Fusarium oxysporum ) is beneficial to increase the yield of cardamom and the life of cardamom plants.

Description

Technical Field

[0001] The invention belongs to the field of microbial fertilizers, and in particular relates to a microbial fertilizer and an application thereof. Background Art

[0002] Microbial fertilizer, also known as biological fertilizer, inoculant or bacterial fertilizer, refers to a type of fertilizer product that uses the life activities of microorganisms as the core to enable crops to obtain specific fertilizer effects. Microbial fertilizer has been widely used in agricultural production in recent years because of its advantages such as improving soil, increasing crop resistance to pests, promoting plant growth, and improving stress resistance.

[0003] Amomum villosum Amomun tsaoko Crevost et Lemarie, also known as cardamom kernel, cardamom fruit, cardamom, is a species of the genus Cardamom in the Zingiberaceae family. Ammonium ) is a perennial evergreen herbaceous economic plant with a spicy taste, warm nature, and non-toxicity. The essential oil extracted from the fruit can be used in the medicine or spice industry; the dried fruit can be used as a Chinese medicinal material, mainly for the prevention and treatment of abdominal distension and pain, fullness and vomiting, malaria, chills and fever, and plague fever. It is included in the "Pharmacopoeia of the People's Republic of China" (2020).

[0004] With its unique spicy flavor, Amomum villosum has become a common seasoning and flavoring ingredient in households and is deeply loved by people. The market demand for Amomum villosum has increased year by year, leading to an increasing area of ​​its cultivation. As a result, the incidence of Amomum villosum root rot has increased, directly affecting the yield of Amomum villosum and the life of Amomum villosum plants. Summary of the invention

[0005] In order to overcome the problems existing in the prior art, the present invention provides a microbial fertilizer and its application. The microbial fertilizer provided by the present invention can effectively prevent and treat the root rot of Amomum villosum.

[0006] To achieve the above object, the present invention provides a microbial fertilizer in a first aspect, comprising the following components:

[0007] Component A: Penicillium purpurogenum ( Penicillium janthinellum ) bacterial suspension and Bacillus mohaivii ( Bacillus mojaveensis ) Bacterial agents;

[0008] Component B: biochar, peat soil, corn straw powder, bentonite, and γ-polyglutamic acid.

[0009] Furthermore, the mass ratio of the biochar, peat soil, corn straw powder, bentonite and γ-polyglutamic acid is: 80-95:300-330:20-30:60-80:0.2-0.8.

[0010] Furthermore, based on the total weight of component B, the effective viable count of Penicillium purpurogenum in the microbial fertilizer is not less than 1×108 CFU / g, the effective viable count of Bacillus mohaiwei is not less than 1×10 8 CFU / g.

[0011] Furthermore, the preparation method of the micropurpurogenous Penicillium suspension is: inoculating the purchased micropurpurogenous Penicillium test tube strain into PDB liquid culture medium, culturing at 26°C-28°C, 180-200r / min, and culturing for 48h to obtain a bacterial suspension.

[0012] Furthermore, the preparation method of the Bacillus mohaiwei bacterial agent is as follows: inoculating the purchased Bacillus mohaiwei test tube strain into NB liquid culture medium for cultivation, and culturing on a shaker at a temperature of 25-28° C. and a rotation speed of 200-220 r / min for 30-35 hours to obtain a liquid strain;

[0013] The liquid strain of Bacillus mohaievii is inoculated into a liquid fermentation medium at a volume ratio of 0.05-0.1:1 for fermentation and culture. The fermentation and culture conditions are: 26°C-28°C, 60%-70% dissolved oxygen, 180-200r / min stirring, and culture for 36-42h to obtain the Bacillus mohaievii inoculum. The liquid fermentation medium is: 95g potato, 80g corn starch, 5g peptone, 12g ammonium chloride, 10g sucrose, and 1000ml water; pH=7.2-7.5.

[0014] The second aspect of the present invention provides the use of the microbial fertilizer of the first aspect in preventing and treating grass fruit root rot.

[0015] Furthermore, the pathogen of the tsaoko root rot is Fusarium oxysporum ( Fusarium oxysporum ).

[0016] A third aspect of the present invention provides a method for preventing and controlling root rot of Amomum villosum, the method comprising applying the microbial fertilizer provided in the first aspect to the rhizosphere of the Amomum villosum plant.

[0017] Through the above technical scheme, the present invention can at least achieve the following beneficial effects: the microbial fertilizer provided by the present invention can effectively prevent and control Fusarium oxysporum ( Fusarium oxysporum ) is beneficial to increase the yield of cardamom and the life of cardamom plants. DETAILED DESCRIPTION

[0018] Unless otherwise specified, the materials and reagents used in the present invention are commercially available.

[0019] In the present invention, Bacillus mohaiwei was purchased from BNCC ® Industrial Microbial Strain Engineering Technology Research Center, its number is: BNCC358077.

[0020] In the present invention, Penicillium purpurogenum was purchased from Beijing Biobo Biotechnology Co., Ltd., and the strain number is: Bio-82047.

[0021] Preparation of Bacillus mohaiwei inoculant:

[0022] The purchased Bacillus mohaiwei test tube strain was inoculated into NB liquid culture medium for cultivation, and cultured on a shaker at a temperature of 25-28° C. and a rotation speed of 200-220 r / min for 30-35 hours to obtain a liquid strain.

[0023] The liquid strain of Bacillus mohaiwei was inoculated into a liquid fermentation medium (95g potato, 80g corn starch, 5g peptone, 12g ammonium chloride, 10g sucrose, 1000ml water; pH=7.2-7.5) at a volume ratio of 0.05-0.1:1 for fermentation culture. The fermentation culture conditions were: 26°C-28°C, 60%-70% dissolved oxygen, 180-200r / min stirring, and cultured for 36-42h to obtain the Bacillus mohaiwei inoculum.

[0024] Preparation of Penicillium purpurogenum suspension:

[0025] The purchased Penicillium purpurogenum test tube strain was inoculated into PDB liquid culture medium (200 g potato, 20 g glucose, 1000 ml water; pH = 6.0-6.5), cultured at 26°C-28°C, 180-200 r / min, and cultured for 48 h to obtain liquid strain.

[0026] Embodiment 1

[0027] A microbial fertilizer comprises the following components:

[0028] Component A: Penicillium purpurogenum ( Penicillium janthinellum ) and Bacillus mohaivii ( Bacillus Mojave );

[0029] Component B: 90kg biochar, 310kg peat soil, 25kg corn straw powder, 70kg bentonite, and 0.5kg γ-polyglutamic acid.

[0030] Based on the total weight of component B, add the Bacillus mohaiwei bacterial agent and the micropurpurogenous Penicillium suspension to the mixed component B, so that the effective viable counts of Bacillus mohaiwei and the micropurpurogenous Penicillium are both about 1×10 8 CFU / g.

[0031] Embodiment 2

[0032] A microbial fertilizer comprises the following components:

[0033] Component A: Penicillium purpurogenum ( Penicillium janthinellum ) and Bacillus mohaivii ( Bacillus Mojave );

[0034] Component B: 80kg biochar, 300kg peat soil, 20kg corn straw powder, 60kg bentonite, and 0.2kg γ-polyglutamic acid.

[0035] Based on the total weight of component B, add the Bacillus mohaiwei bacterial agent and the micropurpurogenous Penicillium suspension to the mixed component B, so that the effective viable counts of Bacillus mohaiwei and the micropurpurogenous Penicillium are both about 1×10 8 CFU / g.

[0036] Embodiment 3

[0037] A microbial fertilizer comprises the following components:

[0038] Component A: Penicillium purpurogenum ( Penicillium janthinellum ) and Bacillus mohaivii ( Bacillus Mojave );

[0039] Component B: 95kg biochar, 330kg peat soil, 320kg corn straw powder, 80kg bentonite, and 0.8kg γ-polyglutamic acid.

[0040] Based on the total weight of component B, add the Bacillus mohaiwei bacterial agent and the micropurpurogenous Penicillium suspension to the mixed component B, so that the effective viable counts of Bacillus mohaiwei and the micropurpurogenous Penicillium are both about 1×10 8 CFU / g.

[0041] Comparative Example 1

[0042] The difference between Comparative Example 1 and Example 1 is that Penicillium purpurogenum is not added to the microbial fertilizer.

[0043] Comparative Example 2

[0044] The difference between Comparative Example 2 and Example 1 is that Bacillus mohaiwei is not added to the microbial fertilizer.

[0045] Comparative Example 3

[0046] The difference between Comparative Example 3 and the embodiment is that Penicillium purpurogenum and Bacillus mohaiwei are not added to the microbial fertilizer.

[0047] Experimental example

[0048] Transplant the cultivated cardamom seedlings into pots, with one plant in each pot, and cultivate them in a greenhouse for one month. Select the cardamom seedlings with good growth, no obvious diseases and similar growth conditions for microbial fertilizer testing.

[0049] The roots of the Amomum villosum plants were dug up a circle of soil (substrate), and the microbial fertilizers of Examples 1 to 3 and Comparative Examples 1 to 3 were applied, with 100 plants in each Example. After the application, the dug-out substrate was covered, and after normal management and cultivation for 15 days, Fusarium oxysporum ( Fusarium oxysporum ) bacterial suspension was used to irrigate the roots of the Amomum villosum plants to which the microbial fertilizers of Examples 1 to 3 and Comparative Examples 1 to 3 were applied; and a group of Fusarium oxysporum plants not subjected to microbial fertilizer irrigated roots was set up. Fusarium oxysporum ) Positive control of bacterial suspension.

[0050] Among them, Fusarium oxysporum ( Fusarium oxysporum ) 200 ml of bacterial suspension per plant of Amomum villosum, the effective number of viable bacteria of Fusarium oxysporum in the bacterial suspension is about 1×10 8 CFU / ml.

[0051] The rest of the management was normal, and the incidence of the grass fruit plants was investigated. The incidence is shown in Table 1 below.

[0052] Incidence rate = number of diseased plants / total number of plants investigated*100%;

[0053] Table 1 The effect of microbial fertilizer on the prevention of root rot of Amomum villosum

[0054]

[0055] As can be seen from Table 1 above, the application of the microbial fertilizer provided by the present invention can effectively prevent and control Fusarium oxysporum ( Fusarium oxysporum ) caused by the root rot of the grass fruit.

[0056] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. A microbial fertilizer for preventing and treating tsaoko root rot, characterized in that: Contains the following components: Component A: Penicillium purpurogenum ( Penicillium janthinellum ) and Bacillus mohaievii ( Bacillus mojavensis ); Component B: biochar, peat soil, corn straw powder, bentonite, and γ-polyglutamic acid.

2. A microbial fertilizer for preventing and treating tsaoko root rot according to claim 1, characterized in that: The mass ratio of the biochar, peat soil, corn straw powder, bentonite and gamma-polyglutamic acid is 80-95:300-330:20-30:60-80:0.2-0.

8.

3. A microbial fertilizer for preventing and treating tsaoko root rot according to claim 1, characterized in that: Based on the total weight of component B, the effective viable count of Penicillium purpurogenum in the microbial fertilizer shall not be less than 1×10 8 CFU / g, the effective viable count of Bacillus mohaiwei is not less than 1×10 8 CFU / g.

4. Use of the microbial fertilizer according to any one of claims 1 to 3 in preventing and treating tsaoko root rot.

5. The use according to claim 4, characterized in that: The pathogen of the grass fruit root rot is Fusarium oxysporum ( Fusarium oxysporum ).

6. A method for preventing and treating tsaoko root rot, characterized in that: Apply the microbial fertilizer as described in any one of claims 1 to 3 to the rhizosphere of the tsaoko plants.

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