Polyphylla polygonatum greenhouse seedling technology based on mechanical peeling cold storage and germination

Abstract

The application discloses a kind of based on mechanical peeling cold storage germination polyphylla prataeshis greenhouse seedling technique, belong to the field of breeding technology.The application uses hexagonal drum screening machine to carry out mechanical peeling to polyphylla prataeshis seed, and stores kernel under the condition of artificial temperature control, humidity control, and the strength is easy to grasp, effectively slows down seed mildew phenomenon, and seed embryo is intact, the seedling method of the patent has the advantages that seed embryo disease infection rate is low (6% or less), seed dormancy time is short (5 months), seedling time is short, germination rate is high (98%), seed treatment capacity is large (one batch can handle 50 jin), operation difficulty is low, growth speed is fast, seedling quality is high, and it is favorable to improve the success rate of large-scale breeding of polyphylla prataeshis.

Description

TECHNICAL FIELD

[0001] The present application relates to a kind of based on mechanical peeling refrigeration germination Polygonatum cyrtonema Greenhouse seedling technology, belong to the breeding technical field. BACKGROUND

[0002] Polygonatum cyrtonema Hua is a perennial herb of Liliaceae Polygonatum, its rhizome is rich in polysaccharide, steroidal saponin, alkaloid, flavone, phytosterol and volatile oil and various active ingredients, so that it has anti-aging, anti-tumor, antibacterial, immune regulation, blood lipid regulation and memory improvement and other pharmacological effects.

[0003] With the development of society, the medicinal health value of Polygonatum cyrtonema is more and more valued by people, and the increasing demand for Polygonatum cyrtonema leads to the plundering of its wild resources, and artificial cultivation is urgently needed. The traditional propagation method of Polygonatum cyrtonema is basically seed and tuber propagation, but the limited amount of tubers cannot meet the development of Polygonatum cyrtonema industry, and the seed has dormancy phenomenon, which greatly restricts the development of Polygonatum cyrtonema industry. In traditional production, the commonly used seed germination method of Polygonatum cyrtonema is room temperature sand storage germination, which has a long dormancy time, and the room temperature sand storage is easy to breed pathogenic bacteria, resulting in seed mold, reducing the quality of the seed, and the labor cost of disease and pest control, water and fertilizer management, weeding and other management in traditional seedling period management is high. The existing technology for the breeding of Polygonatum cyrtonema is limited to small batch breeding, and it is difficult to solve the problems of long seed dormancy time and low seed quality encountered in large-scale breeding. Therefore, it is of great significance to develop a method suitable for large-scale breeding of Polygonatum cyrtonema seed to shorten the seed dormancy time and improve the seed quality. SUMMARY

[0004] The present application proposes a "mechanical peeling low-temperature refrigeration germination large-scale greenhouse seedling technology", which effectively shortens the seed dormancy period, improves the seed germination rate and quality by mechanical sanding seed peeling and low-temperature refrigeration germination, and adopts special seedbed in greenhouse for seedling, which is beneficial to disease and pest control, weed control, soil conservation and reduces the management cost of seedling period, making the production of Polygonatum cyrtonema seedling more and more large-scale and standardized.

[0005] The present application provides a seedling raising method of Polygonatum cyrtonema seed, which is mechanical sanding, then low-temperature preservation, then germination after preservation, and then sowing in special seedbed to obtain Polygonatum cyrtonema seedling; the special seedbed takes coconut husk as the main substrate.

[0006] In one embodiment, the mechanical sanding is to mix the Polygonatum cyrtonema seed with fine sand and then mechanically stir, and the parameters of the mechanical stirring are 30-40 revolutions per minute for 5-15 minutes.

[0007] In an embodiment, the fine sand has a pore size of 0.4-0.5 cm and a water content of 10-15%.

[0008] In an embodiment, the ratio of the Polygonatum sibiricum seed to the fine sand is (1:4) to (1:2) v / v.

[0009] In an embodiment, the low-temperature preservation is at a temperature of 0-5°C for 2-5 months.

[0010] In an embodiment, the special seedbed comprises a lower substrate and an upper substrate; the lower substrate, from bottom to top, is: a nursery original soil high ridge, a geotextile, 1.5-2.5 cm thick of decomposed sterile sheep manure, and 2.5-3.5 cm thick of coconut coir; and the upper substrate is 2.5-3.5 cm thick of coconut coir.

[0011] In an embodiment, the decomposed sterile sheep manure is a base fertilizer.

[0012] In an embodiment, the Polygonatum sibiricum seed is sown between the upper substrate and the lower substrate.

[0013] In an embodiment, the Polygonatum sibiricum seed is mixed with coconut coir at a volume ratio of (1:1) to (1:3) before sowing.

[0014] In an embodiment, the sowing density of the Polygonatum sibiricum seed is 4.5-5.5 kg / 667 m 2 .

[0015] In an embodiment, the seed germination acceleration is: the preserved Polygonatum sibiricum seed and wet coconut coir are mixed at a volume ratio of (1:1) to (1:3), and then placed at a temperature of 15-25°C and a humidity of 40-50% for at least 20 days.

[0016] Advantages:

[0017] (1) Compared with the traditional technology, the present technology effectively improves the seed preservation quality. The Polygonatum cyrtonema seed is mechanically peeled by a hexagonal drum screen, and the seed kernel is stored under artificial temperature and humidity control, which is easy to control and effectively reduces seed mold and rot.

[0018] (2) Effectively breaks seed dormancy and shortens germination time. Using the present technology, the Polygonatum cyrtonema seed dormancy period is 5 months, effectively shortening the seed germination time.

[0019] (3) Effectively improves seed quality and reduces disease infection rate. Using the present technology, the seed germination rate is as high as 98%.

[0020] (4) Effectively prevents insects and weeds, maintains fertility, and reduces management costs.

[0021] (5) effectively reduce the seedling damage rate, easy to seedling transplant, polygonatum sibiricum seedlings gradually towards industrialization, large-scale. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 It is the real photo of mature fruit of polygonatum multiflorum;

[0023] Figure 2 It is the real photo of six hexagonal cylinder screening machine;

[0024] Figure 3 It is the real photo of seed with uncleaned pulp;

[0025] Figure 4 It is the real photo of seed kernel after mechanical sanding and peeling;

[0026] Figure 5 It is the real photo of seedling emergence after mechanical peeling and cold storage;

[0027] Figure 6 It is the real photo of seed germination after mechanical peeling and cold storage for 5 months;

[0028] Figure 7 It is the real photo of demonstration planting in special seedbed;

[0029] Figure 8 It is the comparison chart of weed growth in seedbed with and without coconut husk covering. The left chart is the seedbed without coconut husk covering, and the right chart is the seedbed with coconut husk covering. DETAILED DESCRIPTION

[0030] In the following examples, mechanical treatment of seeds refers to the use of Rongshi single-phase double-value capacitor motor (produced by Shanghai Rongshi Electrical Machinery Co., Ltd. Wenzhou Branch), commonly known as hexagonal cylinder machine, with overall dimensions of 1600mm*1100mm, and internal dimensions of 1200mm*90mm*1200mm. Figure 2 ).

[0031] Example 1

[0032] 1. Collection and processing of fruits

[0033] Select healthy, large and many fruits, no disease and insect pests, strong stress tolerance polygonatum multiflorum plants as seed mother plants, from September to October, with full fruit, fruit peel from green to black as the symbol of seed maturity. The fruits are picked and cleaned, put into foam box for thin layer composting treatment for 10 days, during which the fruit pulp rotting is observed, and when the fruit is rotten and separated from the seed, it is put into the sieve for rubbing, and washed with clean water until the fruit shell and pulp are completely removed. The washed seeds are bright yellow in color, complete in shape, without brown spots, worm holes, mold and peculiar smell. Figure 1 Figure 3

[0034] ​​2. Mechanical sanding of seeds

[0035] The seed coat of Polygonatum sibiricum was subjected to nine different treatments. The first treatment was a seed-sand volume ratio of 1:2, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 5 minutes. The second treatment was a seed-sand volume ratio of 1:2, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 10 minutes. The third treatment was a seed-sand volume ratio of 1:2, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 15 minutes. The fourth treatment was a seed-sand volume ratio of 1:3, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 5 minutes. The fifth treatment was a seed-sand volume ratio of 1:3, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 10 minutes. The sixth treatment was a seed-sand volume ratio of 1:3, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 15 minutes. The seventh treatment was a seed-sand volume ratio of 1:4, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 5 minutes. The eighth treatment was a seed-sand volume ratio of 1:4, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 10 minutes. The ninth treatment was a seed-sand volume ratio of 1:4, a mesh screen aperture of 0.5 cm, a wet sand moisture content of 10-15%, a speed of 30 rpm, and a continuous running time of 15 minutes.

[0036] As shown in Table 1, the seed damage rates of the two treatments were quite different. The seed damage rates of treatments 3 and 9 were 49% and 41%, respectively, while the seed damage rate of treatment 4 was only 5%. Figure 4 Therefore, the optimal mechanical sanding conditions for seeds were a seed-sand volume ratio of 1:3, a wet sand moisture content of 10-15%, a mesh screen aperture of 0.5 cm, a speed of 30 rpm, and a continuous running time of 5 minutes.

[0037] Table 1. Seed damage rates of different seed-sand volume ratios and running times

[0038]

[0039]

[0040] As Figure 4The treated seeds are peeled and the kernels (containing the embryo and endosperm) are exposed. The kernels are rinsed with a mesh screen to remove sand and impurities, soaked in 1% potassium permanganate solution for 20 minutes, and then taken out and slightly aired for 20 minutes in a cool and ventilated place. The surface water is drained, and the safe moisture content is controlled at 8-10% (the appearance is slightly wrinkled on the surface of the kernel).

[0041] 3. Seed low-temperature storage

[0042] The kernels with drained surface water are packed in a breathable woven bag or mesh bag and stored in a seed germination thermostat. The temperature is maintained at 0-5°C, and the kernels are turned over every 3-5 days during the 2-month cold storage. The kernels are sprayed with water to maintain moisture when the surface is wrinkled, and the humidity is maintained at 8-15%. After 1 month of storage, the seed embryo infection rate is only 1%, and after 2 months, the seed embryo infection rate is only 3%, effectively improving the quality of the seeds.

[0043] 4. Room temperature germination

[0044] In early February of the following spring, the seeds are taken out of the thermostat, soaked in 1% potassium permanganate solution for 20 minutes, and then rinsed with clean water for standby. A foam box with a size of 60*60 cm is prepared, coconut husk is added and sprayed with water to wet, the moisture content of the coconut husk is appropriate when the coconut husk is held in a ball and does not drip water, and the coconut husk is scattered when the hand is loosened (the moisture content is about 8-15%), the seeds and coconut husk are mixed at a volume ratio of (1:1) to (1:3), and then placed in an outdoor cool and ventilated place with an open mouth, attention should be paid not to cover a rain shed, and it is strictly forbidden to rain, attention should be paid to regular turning, and timely temperature and humidity maintenance (temperature 24-28°C, humidity 10-15%), and the seeds can germinate after 20 days.

[0045] The cumulative germination rate of the polyfloral polygonatum seed treated by the method reaches 98%. Figure 6 ).

[0046] 5. Land preparation

[0047] (1) Plot selection

[0048] The seedlings should be grown in a shaded shed, and the land should be flat and not accumulate water.

[0049] (2) Land preparation time

[0050] Deep plowing in winter is good, and the plowing depth should be not less than 30 cm. After a winter of freezing, the root rot and underground pest damage during the next year's seedling period can be reduced.

[0051] (3) Special seedbed preparation

[0052] After removing the sundries in the plot, the soil clumps are broken and raked flat, deep plowing is 20-30 cm, and carbendazim 50% wettable powder 10 g / m 2The length of the seedbed is determined by the seeding amount, and is generally not more than 10 meters, the bed height is 25-30 cm, the bed width is 1.2 meters, and the width of the path ditch between the seedbeds is 30 cm.

[0053] Five days before seeding, the whole seedbed, including the manually operated path, is fully covered with a geotextile with a thickness of not less than 0.4 cm, and the original soil substrate is preferably not exposed, and the geotextile is fixed by using a peg. On the set geotextile, about 2 cm of fully decomposed sterile sheep manure is laid as basal fertilizer, and then a layer of about 3 cm of dry coconut husk is laid, and the peg and harrow are used to flatten, so as to serve as the lower layer of the substrate of the special seedbed.

[0054] The thickness of the layer of the sterile sheep manure is adjusted to be 1.5-2.5 cm, and the thickness of the layer of the dry coconut husk is adjusted to be 2.5-3.5 cm, and the technical effects of the present application can also be achieved.

[0055] 6. Seeding

[0056] (1) Seeding

[0057] In early March, the yellow ginseng seeds that have sprouted are sown on the seedbed at a rate of 4.5-5.5 kg per 667 m 2 , and the coconut husk is sown, and the mass ratio of the seeds and the coconut husk is (1:1)-(1:3). Before seeding, the substrate is sprayed with water by using the spraying equipment of the greenhouse, and after seeding, a 2 cm thick coconut husk light substrate (dry coconut husk) is covered and sprayed with water.

[0058] The thickness of the layer of the dry coconut husk is adjusted to be 2.5-3.5 cm, and the technical effects of the present application can also be achieved.

[0059] (2) Germination

[0060] About 15 days after seeding, the yellow ginseng seeds germinate successively, and after one month, the seedlings are basically germinated Figure 5 .

[0061] 7. Seedling stage management

[0062] (1) Water

[0063] During the germination period, the substrate of the seedbed is kept moist, and the humidity is kept at 60%; after the seedlings are germinated, the amount of water spraying is reduced, and the substrate is not dried or sprayed.

[0064] (2) Shading

[0065] The shading net with a shading rate of 50%-70% is used for shading in the seedling greenhouse, and a layer of greenhouse film is covered under the shading net, as shown in Figure 7 .

[0066] (3) Temperature and humidity

[0067] The temperature in the shade shed should be kept at about 15℃, and the shed film should be adjusted according to the temperature on the same day. When the ambient temperature reaches 25℃ or above, spray once a day, preferably at 10am; when the summer temperature reaches 30℃ or above, spray 2 times a day, at 9am and 5pm respectively. If conditions permit, intermittent spraying can be used, but there should be no standing water in the seedbed.

[0068] (4) Fertilization

[0069] After the seedlings' leaves are out, apply a thin layer of fully decomposed organic fertilizer once, and then water it once to prevent disease. Fertilize 4-5 times a year.

[0070] (5) Disease and pest control

[0071] Pests: The bed placement method of the present invention can effectively avoid underground pest damage, and only needs to focus on leaf-eating pests. Use clean water and fertilizers to prevent the spread of insect eggs; regularly clean diseased plants to reduce the source of disease and pests. In addition, physical methods such as manual, light, electricity, and heat can be used to kill or lure pests, avoiding reliance on chemical drugs.

[0072] Diseases: Strengthen water and fertilizer management, promote plant growth, improve resistance, and effectively control disease occurrence.

[0073] Leaf spot disease, mainly harmful to leaves, the initial stage of the disease starts from the base of the leaves, and the leaf surface has faded spots, the disease spot expands to an oval or irregular shape, about 1cm in size, with a light white center and a brown edge, and a clear yellow halo near the healthy tissue. The disease is also called eye spot disease because of the eye-like shape of the disease. When the disease is severe, multiple disease spots coalesce to cause leaf necrosis and shedding, and can gradually spread upwards, eventually causing the whole plant to die and shed leaves. Chemical control: 50% methylthiophanate wettable powder 1000 times liquid, 70% mancozeb wettable powder 600 times liquid, and 75% chlorothalonil wettable powder 600 times liquid can be used for spraying. During drought and low rainfall, the interval period between spraying can be appropriately extended. Spray once every 7-10 days, for 3-5 times in a row.

[0074] Rhizome rot disease, the initial stage of the disease is a light brown circular spot, and the diseased part rots, the tissue is discrete and sunken, the disease spot is circular or oval with a diameter of 5-10mm, and the heavy disease spot connects to form a large block, affecting the yield and quality of polygonatum. Control method: timely root irrigation with medicine at the initial stage of the disease, 50% carbendazim 500 times liquid or 50% fungicide 800 times liquid can be used; timely removal of heavy disease plant roots and complete disinfection with chemicals.

[0075] In summary, the seedling raising method of the present patent has the advantages of low seed embryo pathogen infection rate (6% or less), short seed dormancy time (5 months), short seedling nursery time, high germination rate (98%), large seed treatment capacity (one batch can treat 50 kg), low operation difficulty, fast growth speed, and high quality of seedlings. The seedlings can be nurseryed at the age of 2.5 years (germination and growth in the first year, continuous growth in the second year, and nursery in the third year in spring), and the fresh weight of the tubers is 2.07 g; after 2 months of under-forest planting, the fresh weight of the tubers reaches 4.08 g.

[0076] Effect of different treatment methods on seed embryo pathogen infection rate in Comparative Example 1

[0077] The specific implementation is the same as that in Example 1, except that the traditional room temperature sand storage method and the method of seed mutual collision damage to seed coat disclosed in the patent CN109566371A are used instead of the mechanical peeling and cold storage method of the present application. The results show that the traditional room temperature sand storage method and the method of seed mutual collision damage to seed coat lead to an increase in seed embryo pathogen infection rate, and the seed embryo pathogen infection rates after 2 months are 17% and 42%, respectively. The seed embryo pathogen infection rate after 2 months of mechanical peeling and cold storage in the present application is only 3%. As shown in Table 2.

[0078] Table 2 Comparison of seed embryo pathogen infection rates of different seed treatments and storage methods

[0079] Mode of storage Seedling infection rate (%) after 1 month Seedling infection rate (%) after 2 months Traditional room temperature cold storage 8 17 Mutual collision damage to seed coat 30 42 Mechanical peeling and cold storage according to the present invention 1 3

[0080] Effect of different treatment methods on dormancy period in Comparative Example 2

[0081] The specific implementation is the same as that in Example 1, except that the traditional room temperature sand storage method and the method of seed mutual collision damage to seed coat disclosed in the patent CN109566371A are used instead of the mechanical peeling and cold storage method of the present application. The results show that compared with the present application, the seed dormancy period is prolonged by the room temperature sand storage method and the method of seed mutual collision damage to seed coat, and the seed dormancy time can be effectively shortened by the mechanical peeling and cold storage method of the present application, as shown in Table 3.

[0082] Table 3 Comparison of seed dormancy times of different seed treatments and storage methods

[0083]

[0084] As shown in Table 3, the seed dormancy period of the traditional room temperature sand storage method is 5 months for 44% of the seeds, i.e., the seeds can normally germinate in the following March, and the seed dormancy period of the traditional room temperature sand storage method is 17 months for 43% of the seeds, i.e., the seeds can normally germinate in the third year of March. The seed dormancy period of the low temperature + room temperature method of the existing patent is 14 months, i.e., the seeds can normally germinate in the third year. The seed dormancy period of the mechanical peeling and cold storage method is 5 months, i.e., the seeds can basically germinate in the following March, effectively shortening the seed dormancy time of Polygonatum cyrtonema Hua.

[0085] Effect of different treatment methods on seed germination rate of Comparative Example 3

[0086] The specific implementation refers to Example 1, the difference is that the traditional room temperature sand storage method is used instead of the mechanical impact peeling method described in the application, and the results show that the room temperature sand storage method leads to a significant reduction in seed germination rate, as shown in Table 4.

[0087] Table 4 Comparison of next year's germination rate of different seed treatment and storage methods

[0088] Mode of storage Cumulative germination rate (%) the following year Traditional sand storage in room 44 Mechanical peeling and cold storage 98

[0089] Effect of seedbed substrate on weed coverage, annual manual weeding frequency, and disease and pest infection rate of Comparative Example 4

[0090] The specific implementation refers to Example 1, the difference is that three methods are used for the seedbed of Polygonatum sibiricum, which are:

[0091] Setting 1: The seedbed is all yellow heart soil from bottom to top, and the seed is sown directly into the soil after fine grading under the forest part with a canopy density of 0.2-0.4, and then covered with 3cm thick peat soil and sprayed with water.

[0092] Setting 2: The seedbed is yellow heart soil and 3cm thick peat soil from bottom to top, and the seed is sown directly into the soil after fine grading under the forest part with a canopy density of 0.5-0.7, and then covered with 3cm thick peat soil and sprayed with water.

[0093] Setting 3: The structure of the special seedbed substrate from bottom to top is: 25cm high ridge of original nursery soil, 4mm thick geotextile, 2cm thick mature sterile sheep manure, and 3cm thick coconut husk. The seed is sown directly into the special substrate, covered with 3cm thick coconut husk, and sprayed with water.

[0094] Setting 4: The seed is sown directly in the original soil of the nursery without covering the coconut husk seedbed.

[0095] Table 5 Comparison of weed coverage, annual manual weeding frequency, and disease and pest infection rate of different seedbed substrates

[0096]

[0097] As can be seen from Table 5, the special seedbed of Setting 3 used in this application uses geotextile as the bottom, and sheep manure organic matter ensures the nutrient supply for seedling growth. Coconut husk is used as a light substrate to cover the seedlings, and the upper substrate is isolated from the soil by geotextile. The seedlings are easy to transplant and can effectively prevent soil pests and weed damage. In Setting 4, without covering the coconut husk, there are too many weeds, which is not conducive to management, such as Figure 8The setting 1, 2 - traditional seeding method has low emergence rate and more weeds, the emergence rate is 65%, 73%, the weed coverage rate during the growth period is as high as 68%, 55%, the annual manual weeding is 6 times, 4 times, the plant disease and pest infection rate is 26%, 14%; and the setting 3 - special seedbed has an emergence rate as high as 98% and does not need weeding, the weed control effect is good, and the plant disease and pest infection rate is only 3%, which greatly reduces the management cost. During the management period of the traditional seedbed, the disease and pest control, water and fertilizer management, weeding and the like need to input large manual cost according to the conventional seedling raising method, and it is impossible to form large-scale production. The special seedbed for Polygonatum multiflorum of the present application can effectively prevent the occurrence of underground pests and weeds, meanwhile, it can keep moisture, leach water and ventilate, and provide a high-quality environment for the growth of Polygonatum multiflorum seedlings, the decomposed sheep manure ensures the sheep manure required during the seedling growth period, and the soil conservation and leach water characteristics of the coconut husk also fit the growth characteristics of Polygonatum multiflorum, so that the production of Polygonatum multiflorum seedlings is transformed to large-scale, intensive and efficient.

[0098] Although the present application has been disclosed with the preferred embodiments as above, it is not intended to limit the present application, and any person skilled in the art can make various modifications and modifications without departing from the spirit and scope of the present application, therefore, the protection scope of the present application should be defined by the claims.

Claims

1. A method for cultivating seedlings of Polygonatum sibiricum, characterized in that, The seeds of Polygonatum were mechanically sanded, then stored at low temperature, and then germinated before being sown in a special seedbed to obtain Polygonatum seedlings; the special seedbed used coconut coir as the main substrate. The mechanical sand milling process involves mixing Polygonatum seeds with fine sand and then mechanically stirring the mixture. The parameters for the mechanical stirring are: 30 revolutions per minute for 5 minutes. The fine sand has a pore size of 0.4-0.5 cm and a moisture content of 10-15%. The mixing ratio of Polygonatum seeds to fine sand is 1:3 v / v; The low-temperature preservation temperature is 0-5℃, and the preservation period is 2-5 months; The specialized seedbed includes a lower substrate and an upper substrate; the lower substrate, from bottom to top, consists of: raised ridges of original nursery soil, geotextile, 1.5-2.5 cm thick layer of well-rotted sterile sheep manure, and 2.5-3.5 cm thick layer of coconut coir; the upper substrate is 2.5-3.5 cm thick layer of coconut coir. Polygonatum seeds are sown between the upper and lower substrate layers; Mix the Polygonatum seeds with coconut coir in a volume ratio of (1:1) to (1:3) and then sow them.

2. The method as described in claim 1, characterized in that, The sowing density of Polygonatum seeds is 4.5-5.5 kg / 667m². 2 .

3. The method as described in claim 2, characterized in that, The germination process involves mixing preserved Polygonatum seeds with moist coconut coir at a volume ratio of (1:1) to (1:3) and then placing the mixture at 15-25°C and 40-50% humidity for at least 20 days.

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