A method for cultivating edible fungi based on camphor-type cinnamon residue

Abstract

This invention belongs to the field of edible fungi cultivation technology, specifically relating to a method for cultivating edible fungi based on camphor-type *Cinnamomum camphora* residue. The raw materials used in the cultivation substrate and method of this invention are primarily steam-explosion pretreated camphor residue, ball-milled modified sugarcane bagasse, aged rice bran, and rice straw, supplemented with activated shell powder, sucrose, lime, potassium dihydrogen phosphate, and magnesium sulfate, formulated in appropriate proportions. Rice straw, camphor residue, sugarcane bagasse, and rice bran provide the necessary structure and nutrients for the growth of the substrate; shell powder and potassium dihydrogen phosphate increase the mineral content of the substrate and optimize cultivation conditions; and sucrose promotes mycelial growth. Through the scientific formulation of the above ingredients, the substrate formula of this invention can effectively shorten the cultivation time of edible fungi, increase yield and quality, thereby improving the success rate and economic benefits of edible fungi cultivation.

Description

Technical Field

[0001] This invention belongs to the field of edible fungi cultivation technology, specifically relating to a method for cultivating edible fungi based on camphor-type cinnamon residue. Background Technology

[0002] In recent years, with the increasing demand for healthy diets and nutrition, the market demand for edible fungi has continued to grow, especially for common varieties such as white oyster mushrooms, reishi mushrooms, shiitake mushrooms, and enoki mushrooms. However, the cultivation of edible fungi is highly dependent on traditional cultivation materials, such as sawdust, corn cobs, and rice husks. These materials have limited natural availability and are affected by regional and seasonal production, leading to unstable supply. Furthermore, the increasing frequency of raw material extraction and use has further increased the supply-demand imbalance, pushing up the cost of edible fungi production. This dual pressure of raw material shortages and rising costs severely restricts the development and sustainable development of the edible fungi industry, making it difficult to meet the growing market demand. Therefore, finding new and alternative cultivation materials to reduce production costs and promote the development of the edible fungi industry has become a crucial issue that urgently needs to be addressed to drive its rapid growth.

[0003] *Cinnamomum camphora* is a common native tree species in southern my country, growing in warm, humid, loose, and fertile environments such as Guangdong, Fujian, Guangxi, and Yunnan. Its leaves are rich in various terpenoids, including monoterpenes, diterpenes, and sesquiterpenes. Based on the highest concentration of terpenoids in its leaves, *Cinnamomum camphora* is classified into borneol-type, eucalyptol-type, and caryophyllene-type chemotypes. Among these, the monoterpene borneol, commonly known as camphor or borneol, is a precious southern Chinese medicinal herb (2015 edition of the *Chinese Pharmacopoeia*), possessing antibacterial, antioxidant, and anticancer properties. It is often used as a "guide ingredient" in over 60 kinds of traditional Chinese medicines, such as Compound Danshen Dripping Pills and Quick-Acting Heart-Saving Pills. However, the large amount of camphor residue after essential oil extraction is often not effectively utilized, resulting in resource waste. Applying this residue to edible fungi cultivation would promote its recycling and help solve the problem of shortages in traditional cultivation materials. Summary of the Invention

[0004] To overcome the shortcomings of the existing technology, this invention provides a method for cultivating edible fungi based on camphor-type *Cinnamomum camphora* residue. This method combines various raw materials such as camphor residue and ball-milled modified sugarcane bagasse, and through scientific formulation and optimized preparation process, significantly improves the mycelial growth rate and the yield of edible fungi. This formula utilizes camphor residue after essential oil extraction and modified sugarcane bagasse, overcoming the problem of insufficient traditional cultivation materials, shortening the cultivation cycle to 18-20 days, while enhancing the water retention and nutrient supply of the substrate. It is suitable for large-scale industrial production and has broad application value.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] The first aspect of this invention provides a formulation for edible fungi substrate based on camphor-type cinnamon residue, comprising the following raw materials by weight:

[0007] 95-205 parts rice straw, 450-800 parts steam-explosion pretreated borneol residue, 450-550 parts ball-milled modified sugarcane bagasse, 50-310 parts aged rice bran, 24-26 parts molasses-activated shell powder, 8-10 parts sucrose, 8-10 parts lime, 1-2 parts potassium dihydrogen phosphate, 1-2 parts magnesium sulfate, and 65-70% water.

[0008] The edible mushroom substrate formula of this invention uses steam-explosion pretreated camphor residue, ball-milled modified sugarcane bagasse, aged rice bran, and rice straw as main materials, and activated shell powder, sucrose, lime, potassium dihydrogen phosphate, and magnesium sulfate as auxiliary materials, formulated in appropriate proportions. Rice straw, camphor residue, sugarcane bagasse, and rice bran provide the necessary structure and nutrients for mushroom growth; shell powder and potassium dihydrogen phosphate increase the mineral content of the substrate and optimize cultivation conditions; and sucrose promotes mycelial growth. Through the scientific formulation of the above ingredients, the mushroom substrate formula of this invention can effectively shorten the cultivation time of edible fungi, increase yield and quality, thereby improving the success rate and economic benefits of edible fungi cultivation.

[0009] Preferably, the method for preparing the steam explosion pretreated borneol residue is as follows: using camphor-type borneol as raw material, it is first treated with high pressure and high temperature at 100-500℃ to produce dearomatized borneol residue, then the dearomatized borneol residue is dried at 100-200℃ and fully crushed, and then passed through a 50-200 mesh sieve to obtain the steam explosion pretreated borneol residue.

[0010] Preferably, the method for preparing the ball-milled modified bagasse is as follows: the bagasse is ball-milled for 5-30 minutes at a ball-to-material ratio of 5-20:1 and a ball-milling speed of 2000-8000 rpm.

[0011] Preferably, the method for preparing the aged rice bran is as follows: after passing the rice bran through a 50-200 mesh, it is fermented in a greenhouse for 5-7 days according to the standard of adding 20-200 billion EM bacteria and Bacillus per kilogram of rice bran.

[0012] Preferably, the method for preparing the activated shell powder is as follows: first, add 2-5 parts of molasses to 50-100 parts of shell powder, react at 60-70°C for a period of time, then add 0.4-0.7 parts of hydrochloric acid to the reaction system, react at 30-60°C for 1-2 hours, and collect the solid after the reaction to obtain the activated shell powder.

[0013] Preferably, the aged rice bran, molasses-activated shell powder, and sucrose are free from mold and have a moisture content of 8-12%. The aged rice bran has a particle size of 0.5-1 mm, and the molasses-activated shell powder and sucrose are both in powder form.

[0014] Preferably, the rice straw is free from mold, has a moisture content of 60-70%, is in the form of sawdust, and is 1.0-3.0 cm long and 0.3-0.5 cm wide.

[0015] The second aspect of this invention provides a method for preparing the edible mushroom substrate formula based on camphor-type cinnamon residue as described in the first aspect, comprising the following steps:

[0016] S1. Mixing: After accurately weighing the main and auxiliary materials according to the formula ratio, first mix the main materials rice straw, steam-exploded pretreated borneol residue, ball-milled modified sugarcane bagasse, aged rice bran, and activated shell powder according to the material-to-water ratio of total raw material mass: water = 3-5:7-10, and stir repeatedly until evenly mixed. Separately, dissolve the other auxiliary materials sucrose, lime, potassium dihydrogen phosphate, and magnesium sulfate in water in sequence until fully dissolved to obtain a mixed auxiliary material aqueous solution. Finally, stir the main materials and the mixed auxiliary material aqueous solution evenly until they can be formed into a ball by hand but crumble easily when gently placed, thus obtaining a culture medium with a pH of 6-6.5.

[0017] S2. Bagging: Pack the culture medium obtained in step S1 into a high-pressure high-density polyethylene bag. Insert a punching rod into one end of the bag. When filling, make sure to pack it tightly so that there is no finger indentation when you squeeze it lightly. This will give you the mushroom sticks to be sterilized.

[0018] S3. Sterilization: The mushroom sticks need to be sterilized on the same day using high-pressure steam sterilization at 120-121℃ and 0.1-0.13Mpa for 3-4 hours.

[0019] S4. Cooling: Transfer the sterilized mushroom cultivation bags obtained in step S3 to the strong exhaust cooling chamber in a timely manner. Inoculate when the cultivation bags have cooled to room temperature.

[0020] S5. Inoculation: Inoculation must be completed within 24 hours in an inoculation box or a clean bench. The inoculation method is to first wipe your hands and inoculation tweezers with disinfectant alcohol, take out the punching stick from the inoculation end of the mushroom stick, use tweezers to inoculate the solid edible mushroom spawn into the hole, fill the entire hole, and finally seal it with sterile sealing film.

[0021] S6. Mycelium growth and cultivation: After inoculation, the cultivation bags are transferred to the cultivation room for mycelium growth. During the mycelium growth period, the temperature is controlled at 24-26℃ and the humidity is controlled at 85%-95%. The cultivation is carried out in a dark environment with ventilation for 30-60 minutes every day. After 6-7 days of inoculation, the germination and contamination of the mycelium are checked. Ungerminated and contaminated mycelium blocks are removed in time. The entire mycelium growth process takes 18-20 days. Once the mycelium has fully grown, the edible mushroom blocks are ready for cultivation.

[0022] S7. Harvesting: Use a knife to cut the mycelial cords from the bottom of the volva. Do not pull directly and damage the mycelial cords. After harvesting, peel off the volva and gently rotate until the cap and stem separate. Place the stem and cap separately.

[0023] Preferably, the punch rod described in S2 has a diameter of 1.8-2.2 cm and a length of 14-16 cm, with a hole at the tip that is filled with non-degreased cotton.

[0024] Preferably, the sealing film in S5 is a high-temperature resistant tissue culture sealing film.

[0025] Compared with the prior art, the beneficial effects of the present invention are:

[0026] The cultivation substrate formula and method for cultivating edible fungi provided by this invention use a culture medium primarily composed of steam-explosion pretreated borneol residue and ball-milled modified sugarcane bagasse, supplemented by aged rice bran, activated shell powder, etc. The modification effects of each component are as follows:

[0027] Through high-temperature and high-pressure steam explosion, the antibacterial components (such as camphor-type aromatic oil) in the borneol residue are effectively removed, while the fibrous structure is partially deconstructed, making it looser and increasing porosity, significantly improving the substrate's air permeability and water retention. This structural change not only enhances the retention capacity of water and nutrients but also provides an ideal microenvironment for rapid mycelial expansion. Furthermore, the nutrients in the residue after steam explosion are more easily absorbed by the mycelium, thereby accelerating mycelial germination and growth and shortening the cultivation cycle. For edible mushroom cultivation, the borneol residue after steam explosion provides an excellent substrate environment, which not only promotes healthy mycelial growth but also improves cultivation efficiency and yield, while being environmentally friendly and sustainable, making it suitable for large-scale industrial production of edible mushrooms.

[0028] After being ball-milled under high-speed shear force, sugarcane bagasse has a refined fibrous structure, more uniform particles, and a significantly increased specific surface area. This modification enhances the water absorption and aeration of the bagasse, allowing it to better retain moisture when used as a substrate for edible mushroom cultivation, thus providing the humid environment necessary for mycelial growth. Furthermore, ball milling improves nutrient accessibility, making it easier for mycelia to absorb organic matter and trace elements. Therefore, ball-milled sugarcane bagasse, as a substrate, can promote rapid mycelial growth, shorten the cultivation cycle, increase the yield and quality of edible mushrooms, and enhance the stability and sustainability of the substrate, providing an excellent choice for large-scale production.

[0029] Aged rice bran, after long-term storage and fermentation, has more stable nutritional components, especially its protein and fat decomposition products, which are easily absorbed and utilized by edible fungi. Through fermentation, the organic matter in aged rice bran is transformed, enriching it with amino acids, vitamins, and trace elements, thus enhancing its fertilizing effect. Simultaneously, fermented rice bran has good water absorption and aeration properties, helping to provide sufficient moisture and oxygen for the mycelium. These characteristics make aged rice bran a high-quality nutrient source for cultivating edible fungi, promoting rapid mycelial growth, increasing yield, and improving the quality of the fungi. Furthermore, the rice bran fermentation process can inhibit the growth of harmful bacteria, reducing the risk of contamination during cultivation.

[0030] The addition of molasses promotes microbial activity and enhances the organic matter content of the shell powder. Acid treatment further activates the calcium in the shell powder, improving its solubility and bioavailability in the substrate. This process not only improves the pH regulation ability of the shell powder but also increases its efficiency in providing minerals such as calcium and magnesium. When used for cultivating edible fungi, activated shell powder effectively improves the substrate's acid-base balance, optimizes the mycelial growth environment, enhances the substrate's mineral supply, promotes healthy mycelial development and high-quality growth of edible fungi, and increases yield and quality. Furthermore, the calcium ions in the shell powder can strengthen the structural stability of the mycelium, further improving cultivation results.

[0031] In summary, the formula and method for cultivating edible fungi based on borneol residue provided by this invention achieves efficient utilization of forestry and agricultural waste by using borneol residue after essential oil extraction, ball-milled modified sugarcane bagasse, and aged rice bran. This reduces reliance on traditional cultivation materials and lowers production costs. The addition of acid and molasses-activated shell powder enhances the mineral supply and pH adjustment capacity of the substrate, ensuring a stable growth environment for mycelium and further improving the yield and quality of edible fungi. Furthermore, this invention provides a standardized process for cultivating edible fungi using this method, making it suitable for large-scale industrial production, possessing significant economic benefits, and promoting the high-quality development of the edible fungi industry. Detailed Implementation

[0032] The specific embodiments of the present invention will be further described below. It should be noted that these descriptions are for the purpose of aiding understanding the present invention, but do not constitute a limitation thereof. Furthermore, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0033] Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods, and the experimental materials used in the following embodiments are all available through conventional commercial channels.

[0034] Example 1

[0035] This embodiment provides a cultivation substrate formula for edible fungi, which includes the following raw materials by weight:

[0036] The ingredients are: 95-205 parts rice straw, 450-800 parts steam-explosion pretreated camphor residue, 450-550 parts ball-milled modified sugarcane bagasse, 50-310 parts aged rice bran, 24-26 parts activated shell powder, 8-10 parts sucrose, 8-10 parts lime, 1-2 parts potassium dihydrogen phosphate, 1-2 parts magnesium sulfate, and 65-70% water (total raw material mass + water = 100%). The rice bran, shell powder, and sucrose are free of mold and have a moisture content of 8-12%. The rice bran has a particle size of 0.5-1 mm, and the shell powder and sucrose are in powder form. The rice straw is free of mold, has a moisture content of 60-70%, and is in sawdust form, 1.0-3.0 cm long and 0.3-0.5 cm wide.

[0037] The preparation method of steam explosion pretreated borneol residue is as follows: using borneol-type borneol as raw material, it is first treated at 300℃ high pressure and high temperature to produce dearomatized borneol residue, then dried at 150℃ and fully crushed, and then passed through a 100-mesh sieve to obtain steam explosion pretreated borneol residue.

[0038] The method for preparing ball-milled modified bagasse is as follows: the bagasse is ball-milled for 20 minutes at a ball-to-material ratio of 10:1 and a ball-milling speed of 5000 rpm.

[0039] The method for preparing the aged rice bran is as follows: after passing the rice bran through a 100-mesh sieve, it is fermented at room temperature for 6 days with 10 billion mixed bacteria (a mixture of EM bacteria and Bacillus) added per kilogram of rice bran. The EM bacteria and Bacillus mixed inoculant used was purchased from Aimul Environmental Biotechnology (Nanjing) Co., Ltd.

[0040] The method for preparing the activated shell powder is as follows: First, add 4 parts of molasses to 80 parts of shell powder, react at 65°C for 1 hour, then add 0.5 parts of hydrochloric acid to the reaction system, react at 50°C for 1 hour, and collect the solid after the reaction to obtain the activated shell powder.

[0041] The method for preparing the cultivation substrate includes the following steps;

[0042] (1) Mixing: After accurately weighing the main materials and auxiliary materials according to the formula ratio, first mix the main materials rice straw, steam-exploded pretreated borneol residue, ball-milled modified sugarcane bagasse, aged rice bran and activated shell powder according to the material-to-water ratio of total raw material mass: water = 3:7, and mix them repeatedly until evenly mixed. In addition, dissolve the other auxiliary materials sucrose, lime, potassium dihydrogen phosphate and magnesium sulfate in water in sequence until they are fully dissolved to obtain a mixed auxiliary material aqueous solution. Finally, mix the main materials and the mixed auxiliary material aqueous solution evenly until they can be formed into a ball by hand and crumble easily when gently placed. This is the culture medium (pH 6-6.5).

[0043] (2) Bagging: The culture medium obtained in step (1) is packed into a high-pressure high-density polyethylene bag (the high-pressure high-density polyethylene bag is 160cm×35cm×0.65cc in size and weighs 1±0.05kg after packing). A perforated stick (the size is 1.8-2.2cm in diameter and 14-16cm in length, with a hole at the tip, and filled with non-degreased cotton) is inserted into one end of the bag. When packing, it is necessary to pack it tightly so that there is no indentation when the hand is lightly squeezed. This is how the mushroom sticks to be sterilized are obtained.

[0044] (3) Sterilization: It needs to be sterilized on the same day by using high-pressure steam sterilization at 120-121℃ and 0.1-0.13Mpa for 3-4 hours.

[0045] (4) Cooling: Transfer the sterilized spawn bags obtained in step (3) to the strong exhaust cooling chamber in a timely manner. Inoculate when the spawn bags cool down to room temperature.

[0046] (5) Inoculation: Inoculation must be completed within 24 hours in an inoculation box or a clean bench. The inoculation method is to first wipe your hands and inoculation tweezers with disinfectant alcohol, take out the punching stick from the inoculation end of the mushroom stick, and use tweezers to insert the solid edible fungi spawn (such as white oyster mushroom, Ganoderma lucidum, etc., purchased from the National Edible Fungi Engineering Technology Research Center) into the hole, filling the entire hole, and finally seal it with a sterilized 12×12cm high-temperature resistant tissue culture sealing film.

[0047] (6) Mycelium growth: After inoculation, the cultivation bags are transferred to the cultivation room for mycelium growth. The temperature during the mycelium growth period is controlled at 24-26℃ and kept dry (humidity 85%-95%). The bags are cultivated in a dark environment and ventilated for 30-60 minutes every day. After 6-7 days of inoculation, the germination and contamination of the mycelium are checked. Any non-germinating or contaminated bags are removed in time. The entire mycelium growth process takes 18-20 days. Once the mycelium has grown fully, the edible mushroom bags are ready for cultivation.

[0048] (7) Harvesting: Use a knife to cut the mycelial cord from the bottom of the volva. Do not pull directly to avoid damaging the mycelial cord. After harvesting, peel off the volva and gently rotate until the cap and stem fall off. Place the stem and cap separately.

[0049] The following example uses white oyster mushrooms to demonstrate in detail the effects of the cultivation substrate formula of this embodiment on the cultivation of edible fungi (cultivation conditions: soilless, humidity 80%, temperature 25%):

[0050] As shown in Table 1, the mushroom substrate prepared using the cultivation substrate formula and method of this invention yields a higher first crop of oyster mushrooms compared to the existing white oyster mushroom substrate formula (Chu Xiaozhen et al. 2024). Furthermore, the cultivation time is reduced by 40% compared to the traditional white oyster mushroom substrate formula.

[0051] Table 1. Comparison of the first-crop yield of the oyster mushroom culture medium of the present invention with that of existing oyster mushroom culture media.

[0052]

[0053]

[0054] Note: Chu Xiaozhen, Liu Xuan, Gong Pan, Liu Ge, Wang Baorui, Gao Xiang (2024): Effects of different substrates on the growth, development, nutritional components and mineral content of oyster mushroom. Chinese Vegetables 37, 90-95.

[0055] Example 2

[0056] This embodiment provides a cultivation substrate formula for edible fungi, and the formula and preparation method of the substrate are the same as those in Example 1.

[0057] The following example uses Ganoderma lucidum to demonstrate in detail the effect of the cultivation substrate formula of this embodiment on the cultivation of edible fungi (cultivation conditions are soilless, humidity 80%, temperature 25%):

[0058] As shown in Table 1, the mushroom substrate prepared using the cultivation substrate formula and method of this invention yields a higher first crop of Ganoderma lucidum compared to existing Ganoderma lucidum substrate formulas (Shu Tinghui, 2023). Furthermore, the cultivation time is reduced by 24.4% compared to traditional Ganoderma lucidum substrate formulas.

[0059] Table 2 Comparison of the first-crop yield of the Ganoderma lucidum culture medium of the present invention and existing Ganoderma lucidum culture media.

[0060]

[0061]

[0062] Note: Shu Tinghui (2023): Effects of different cultivation materials on the growth and nutritional quality of Ganoderma lucidum fruiting bodies. Special Economic Plants and Animals 26, 39-41.

[0063] In summary, the edible mushroom substrate formula and preparation method provided by this invention, by rationally utilizing the borneol-type euphorbia resin extract residue as part of the cultivation substrate, not only overcomes the traditional problem of euphorbia resin residue inhibiting bacteria, but also fully utilizes its unique fiber structure and nutritional value. Combined with the rational combination of other cultivation ingredients, this novel substrate can significantly accelerate the growth of white oyster mushroom and Ganoderma lucidum mycelium, greatly shortening the cultivation cycle. At the same time, this cultivation method also effectively increases the yield of edible mushrooms, providing a more efficient solution for industrial production.

[0064] The embodiments of the present invention have been described in detail above, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and these variations still fall within the protection scope of the present invention.

Claims

1. A formula for edible mushroom substrate based on camphor-type *Cinnamomum camphora* residue, characterized in that, By weight, it includes the following ingredients: The ingredients are: 95-205 parts rice straw, 450-800 parts steam-explosion pretreated camphor residue, 450-550 parts ball-milled modified sugarcane bagasse, 50-310 parts aged rice bran, 24-26 parts molasses-activated shell powder, 8-10 parts sucrose, 8-10 parts lime, 1-2 parts potassium dihydrogen phosphate, 1-2 parts magnesium sulfate, and 65-70% water; the rice straw is free of mold, has a moisture content of 60-70%, is sawdust-like, 1.0-3.0 cm long, and 0.3-0.5 cm wide. The preparation method of the steam explosion pretreated borneol residue is as follows: using camphor-type borneol as raw material, it is first treated with high pressure and high temperature at 100-500℃ to produce dearomatized borneol residue, then dried at 100-200℃ and fully pulverized, and then passed through a 50-200 mesh sieve to obtain the steam explosion pretreated borneol residue; the preparation method of the ball mill modified sugarcane bagasse is as follows: the sugarcane bagasse is ball milled for 5-30 minutes at a ball-to-material ratio of 5-20:1 and a ball milling speed of 2000-8000 rpm; The preparation method of aged rice bran is as follows: after passing the rice bran through a 50-200 mesh, ferment it in a greenhouse for 5-7 days according to the standard of adding 20-200 billion EM bacteria and Bacillus per kilogram of rice bran; the preparation method of activated shell powder is as follows: first, add 2-5 parts of molasses to 50-100 parts of shell powder, react at 60-70℃ for a period of time, then add 0.4-0.7 parts of hydrochloric acid to the reaction system, react at 30-60℃ for 1-2 hours, and collect the solid after the reaction to obtain activated shell powder.

2. The edible mushroom substrate formula based on camphor-type osmanthus residue according to claim 1, characterized in that, The aged rice bran, molasses-activated shell powder, and sucrose are free from mold and have a moisture content of 8-12%. The particle size of the aged rice bran is 0.5-1 mm, and the molasses-activated shell powder and sucrose are both in powder form.

3. The preparation method of the edible mushroom substrate formula based on camphor-type *Cinnamomum camphora* residue as described in claim 1 or 2, characterized in that, Includes the following steps: S1. Mixing: After accurately weighing the main and auxiliary materials according to the formula ratio, first mix the main materials rice straw, steam-exploded pretreated borneol residue, ball-milled modified sugarcane bagasse, aged rice bran, and activated shell powder according to the material-to-water ratio of total raw material mass: water = 3-5: 7-10, and stir repeatedly until evenly mixed. Separately, dissolve the other auxiliary materials sucrose, lime, potassium dihydrogen phosphate, and magnesium sulfate in water in sequence until fully dissolved to obtain a mixed auxiliary material aqueous solution. Finally, stir the main materials and the mixed auxiliary material aqueous solution evenly until they can be formed into a ball by hand but crumble easily when gently placed, thus obtaining a culture medium with a pH of 6-6.

5. S2. Bagging: Pack the culture medium obtained in step S1 into a high-pressure high-density polyethylene bag. Insert a punching rod into one end of the bag. When filling, make sure to pack it tightly so that there is no finger indentation when you squeeze it lightly. This will give you the mushroom sticks to be sterilized. S3. Sterilization: The mushroom sticks need to be sterilized on the same day using high-pressure steam sterilization at 120-121℃ and 0.1-0.13Mpa for 3-4 hours. S4. Cooling: Transfer the sterilized mushroom cultivation bags obtained in step S3 to the strong exhaust cooling chamber in a timely manner. Inoculate when the cultivation bags have cooled to room temperature. S5. Inoculation: Inoculation must be completed within 24 hours in an inoculation box or a clean bench. The inoculation method is to first wipe your hands and inoculation tweezers with disinfectant alcohol, take out the punching stick from the inoculation end of the mushroom stick, use tweezers to inoculate the solid edible mushroom spawn into the hole, fill the entire hole, and finally seal it with sterile sealing film. S6. Mycelium growth and cultivation: After inoculation, the cultivation bags are transferred to the cultivation room for mycelium growth. During the mycelium growth period, the temperature is controlled at 24-26℃ and the humidity is controlled at 85%-95%. The cultivation is carried out in a dark environment with ventilation for 30-60 minutes every day. 6-7 days after inoculation, the germination and contamination of the mycelium are checked. Ungerminated and contaminated mycelium blocks are removed in time. The entire mycelium growth process takes 18-20 days. Once the mycelium has fully grown, the edible mushroom substrate blocks are ready for cultivation. S7. Harvesting: Use a knife to cut the mycelial cords from the bottom of the volva. Do not pull directly and damage the mycelial cords. After harvesting, peel off the volva and gently rotate until the cap and stem separate. Place the stem and cap separately.

4. The preparation method of the edible mushroom substrate formula based on camphor-type cinnamon residue according to claim 3, characterized in that, The punch rod described in S2 has a diameter of 1.8-2.2cm and a length of 14-16cm, with a hole at the tip that is filled with non-degreased cotton.

5. The preparation method of the edible mushroom substrate formula based on camphor-type cinnamon residue according to claim 3, characterized in that, The sealing film mentioned in S5 is a high-temperature resistant tissue culture sealing film.

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