A method for establishing an artificial feeding model in rats

Abstract

The present invention discloses a method for establishing an artificial feeding model for rats, which belongs to the field of biomedical technology. Specifically: 1) select a number of newly born SD suckling mice and divide them into an artificial feeding group and a breast feeding group on average; 2) prepare artificial milk; artificially feed each SD suckling mouse in the artificial feeding group through a suckling mouse feeding device; 3) the SD suckling mice and the mother mice in the breast feeding group are placed in the same space without artificial intervention; 4) the artificial feeding group and the breast feeding group are continued until the weaning period, and the weight growth curve of the SD suckling mice is drawn. If there is no significant statistical difference between the weight growth curve of the artificial feeding group and the breast feeding group, and the mortality rate is less than 0.1%, the artificial feeding suckling mouse model is successfully established. This model provides a feasible animal model support for exploring the impact of environmental factors in early life on the health outcomes of individuals at different life development stages, and can deeply explore the potential mechanism of the impact of breastfeeding on individual growth and development compared with formula feeding.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine, and particularly relates to a method for establishing an artificial feeding model of rats. Background Art

[0002] The Doha Theory (DOHaD), also known as the "Developmental Origins of Health and Disease" theory, explains the impact of environmental factors, especially nutrition, within the first 1,000 days of life on adult health. Many epidemiological studies have found that it is related to the risk of certain adult non-communicable diseases, such as hypertension, type 2 diabetes, cardiovascular disease, and neuropsychiatric diseases.

[0003] It is worth noting that the family upbringing environment is an important environmental factor affecting individual health. According to the theory of early childhood development, early life is a critical period for the establishment of eating behavior. However, the mechanism by which breastfeeding affects the growth and development of offspring is still unclear, and there is no unified and standardized exploration model. Therefore, by intervening in the dietary habits of suckling mice in early life, an anthropomorphic infant feeding method can be constructed to simulate the early feeding methods of human infants in small mammal populations. Subsequently, by strictly controlling the confounding factors in the growth and development process, the focus is on exploring the impact of feeding methods on the occurrence and development of obesity in children, adolescents and adults. Summary of the Invention

[0004] The purpose of the present invention is to solve the deficiencies in the prior art and provide a method for establishing an artificial feeding model in rats.

[0005] The specific technical solutions adopted in the present invention are as follows:

[0006] The present invention provides a method for establishing an artificial feeding model of rats, which comprises the following steps:

[0007] S1: Several newborn SD rats were selected and divided equally into artificial feeding group and breast feeding group;

[0008] S2: Prepare artificial milk; artificially feed each SD rat in the artificial feeding group using the rat feeding device: first suck the artificial milk into the rat feeding device, empty the air inside, and then perform gavage on the SD rat; during the feeding process, ensure that the head, neck and body of the SD rat are in a straight line, and the head is slightly higher than the body; the amount of artificial milk fed each time Where: W is the weight of SD rats, in grams; H is the feeding amount, in mL;

[0009] After the scheduled feeding is completed, observe the SD rat for 30-40 seconds. If the body color and breathing are normal, continue feeding the next SD rat until all the SD rats in the artificial feeding group have been fed.

[0010] S3: SD rats and mother rats in the breast-fed group were placed in the same room without human intervention;

[0011] S4: Both the artificial feeding group and the breast feeding group continue until the SD rats are weaned, and the weight growth curves of the SD rats in the artificial feeding group and the breast feeding group are drawn. If the weight growth curve of the artificial feeding group is not lower than that of the breast feeding group, and the mortality rate of the SD rats in the artificial feeding group is lower than 0.1%, the offspring obese rat model is successfully established.

[0012] Preferably, the suckling mouse feeding device is made by connecting a medical peripherally inserted central venous catheter with a diameter of 0.62 mm and a disposable medical sterile syringe needle.

[0013] Preferably, goat milk powder is dissolved in sterilized water to prepare artificial milk, wherein the concentration of goat milk powder is 300-350 g / L; the prepared artificial milk needs to be kept in the range of 35-40° C. to avoid indigestion caused by the artificial milk being too low in temperature.

[0014] Preferably, each SD rat in the artificial feeding group is fed 5 to 6 times a day, with an interval of 1.5 to 2 hours between each feeding.

[0015] Preferably, during the daily feeding period of the artificial feeding group, the abdomen of each SD suckling mouse was massaged to stimulate urination and defecation 3 to 4 times.

[0016] Preferably, both the artificial feeding group and the breastfeeding group are continued for 28 days.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] (1) The present invention innovatively proposes a method for artificially feeding newborn SD rats. After artificial feeding begins, the weight and mortality of the experimental animals are tracked and recorded daily. Their general physiological activity, such as appearance, hair growth, and mental state, are observed and recorded. A weight growth curve is then plotted to compare the weight change trends of the SD rats in the breast-fed and artificial-fed groups. It was found that the weight growth curve of the artificial-fed group was not lower than that of the breast-fed group, and the mortality rate of the SD rats in the artificial-fed group was less than 0.1%.

[0019] (2) The present invention utilizes the above-mentioned artificial feeding method to establish an animal model that simulates the feeding environment of early human infants. It was found that the artificially fed rats had good growth and development from the fetal period to adulthood, and there was no significant difference in weight, survival rate, etc. from their breast-fed offspring. This provides a feasible animal model to support the impact of early life environmental factors on different health outcomes of individuals. It has theoretical significance and application value for in-depth research on the impact of breastfeeding and different family rearing environments on the occurrence and development of health outcomes at different stages of life. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A flow chart of the method for establishing the artificial feeding model of rats provided by the present invention;

[0021] Figure 2 This is the weight curve of SD rats in the artificial feeding group and breast-fed group at different growth and development stages;

[0022] Figure 3 Figure 2 is the body length curve of SD rats in the artificial feeding group and breastfeeding group at different growth and development stages. DETAILED DESCRIPTION

[0023] The present invention will be further described and illustrated below with reference to the accompanying drawings and specific embodiments. The technical features of each embodiment of the present invention may be combined accordingly, provided that there is no conflict between them.

[0024] Example

[0025] This embodiment provides a method for establishing an artificial feeding model in rats, and the specific steps are as follows: Figure 1 ):

[0026] (1) A peripherally inserted central venous catheter (PICC tube) with a diameter of 0.62 mm was connected to a 1 mL disposable medical sterile syringe needle to make a suckling mouse feeding device.

[0027] (2) Several newborn SD rats were selected and divided equally into an artificial feeding group and a breast feeding group.

[0028] (3) Weigh the SD rats in the artificial feeding group in advance. Prepare an appropriate amount of artificial milk by dissolving 1g of goat milk powder in 3ml of sterile high-pressure water. After preparation, place the artificial milk on a heat pad and keep it between 35 and 40°C to prevent the artificial milk from being too cold and causing indigestion in the rats.

[0029] (4) Artificial feeding was performed on each SD rat in the artificial feeding group using a rat feeding device: a syringe filled with artificial milk was placed in a warm water bag, and then the SD rat's forelimbs were fixed by hand so that its head was in the mouth of the feeder, and its head, neck and body were in a straight line, with the head slightly higher than the body.

[0030] Suck artificial milk into the suckling mouse feeding device, empty the internal air and perform gavage on the SD suckling mouse: first drop a small amount of artificial milk on the lips of the SD suckling mouse to induce it to open its mouth and lick it, then gently insert the tube into the mouth of the SD suckling mouse. As the SD suckling mouse sucks, the PICC tube will go straight into the esophagus and into the stomach, then slowly push the artificial milk in until the predetermined amount is reached, and then slowly and steadily pull out the PICC tube.

[0031] After the predetermined amount of feeding is completed, the SD rat is observed for 30-40 seconds. If the body color and breathing are normal, the next SD rat is fed until all the SD rats in the artificial feeding group are fed.

[0032] Artificial feeding was performed 6 times a day at 9:00, 10:30, 12:00, 13:30, 15:00, and 16:30. The amount of artificial milk fed each time was Where: W is the weight of SD rats, in grams; H is the feeding amount, in mL.

[0033] In the artificial feeding group, the abdomen of each SD rat was massaged three times during the daily feeding period to stimulate urination and defecation.

[0034] (5) The SD rats and mother rats in the breast-fed group were placed in the same room without human intervention.

[0035] (6) Both the artificial feeding group and the breast feeding group continued until the 28th day of the SD rat weaning period, and the weight growth curves of the SD rats in the artificial feeding group and the breast feeding group were drawn.

[0036] In this example, if the weight growth curve of the artificially fed group within 28 days is not lower than that of the breast-fed group, and the mortality rate of the SD mice in the artificially fed group is lower than 0.1% (the mortality rate of the breast-fed group is 0.25%), then the artificially fed suckling mouse model is successfully established. This indicates that the artificial feeding method provided by the present invention is operationally feasible and has data support.

[0037] In order to verify the weight changes of the artificially reared and breastfed mice groups after adulthood, the mice in the artificially reared and breastfed groups were housed in separate cages 28 days later. The feeding method was as follows: the weaned mice were randomly divided into groups and housed together with four mice per cage. They were supplied with sufficient ordinary feed and autoclaved water every day to ensure their free diet and normal circadian rhythm.

[0038] Figure 2 The weight curves of SD rats in the artificial feeding group and breast-fed group at different growth and development stages. Figure 2 It can be seen that the weight of the suckling mice in the artificial feeding group was slightly higher than that of the breast-fed group starting from the late weaning period (i.e. 21 days of age). This shows that there was no significant difference in weight gain between the artificial feeding group and the breast-fed group from infancy to adulthood, and the weight gain was slightly higher than that of the breast-fed group, indicating that their growth and development were in good condition.

[0039] Figure 3 The body length curves of SD rats in the artificial feeding group and breast-fed group at different growth and development stages. Figure 3It can be seen that there was no significant difference in body length between the artificially fed group and the breast-fed group after weaning (i.e., 28 days of age). This shows that there was no significant difference in body length growth between the artificially fed group and the breast-fed group from the late infancy to adulthood, indicating that their height development and growth were in good condition.

[0040] The embodiment described above is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Persons skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, any technical solution obtained by equivalent substitution or equivalent transformation falls within the scope of protection of the present invention.

Claims

1. A method for establishing an artificial feeding model of rats, characterized in that: The specific steps are as follows: S1: Several newborn SD rats were selected and divided equally into artificial feeding group and breast feeding group; S2: Prepare artificial milk; artificially feed each SD rat in the artificial feeding group using the rat feeding device: first suck the artificial milk into the rat feeding device, empty the air inside, and then perform gavage on the SD rat; during the feeding process, ensure that the head, neck and body of the SD rat are in a straight line, and the head is slightly higher than the body; the amount of artificial milk fed each time ;W is the weight of SD rats, in grams, is the feeding amount, in mL; After the scheduled feeding is completed, observe the SD rat for 30-40 seconds. If the body color and breathing are normal, continue feeding the next SD rat until all the SD rats in the artificial feeding group have been fed. S3: SD rats and mother rats in the breast-fed group were placed in the same room without human intervention; S4: Both the artificial feeding group and the breast-fed group continue until the SD rats are weaned. The weight growth curves of the SD rats in the artificial feeding group and the breast-fed group are drawn. If the weight growth curve of the artificial feeding group is not lower than that of the breast-fed group, and the mortality rate of the SD rats in the artificial feeding group is lower than 0.1%, the offspring obese rat model is successfully established; In the artificial feeding group, each SD rat was fed 5 to 6 times a day, with an interval of 1.5 to 2 hours between each feeding.

2. The method for establishing a rat artificial feeding model according to claim 1, wherein The suckling mouse feeding device is made by connecting a medical peripherally punctured central venous catheter with a diameter of 0.62 mm and a disposable medical sterile syringe needle.

3. The method for establishing a rat artificial feeding model according to claim 1, wherein Prepare artificial milk by dissolving goat milk powder in sterilized water, where the concentration of goat milk powder is 300-350 g / L; the prepared artificial milk needs to be kept within the range of 35-40°C to avoid indigestion caused by the artificial milk being too low in temperature.

4. The method for establishing a rat artificial feeding model according to claim 1, wherein In the artificial feeding group, the abdomen of each SD rat was massaged 3 to 4 times daily during feeding to stimulate urination and defecation.

5. The method for establishing a rat artificial feeding model according to claim 1, wherein Both the artificial feeding group and the breastfeeding group lasted for 28 days.

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