Binary compound bactericidal composition and application thereof

Abstract

The invention belongs to the field of pesticides, and particularly relates to a binary compound bactericidal composition and application thereof. The composition is prepared from the flurobixafen and the tebuconazole. According to the composition disclosed by the invention, the dosage of chemical pesticides is greatly reduced after the flurobixafen and the tebuconazole are compounded, the prevention and treatment effect is far higher than that of a single agent, and the composition has the effects of reducing cost and improving efficiency.

Description

Technical Field

[0001] The invention belongs to the field of pesticides, and specifically relates to a binary compound fungicide composition and application thereof. Background Art

[0002] Wheat is one of the important food crops in my country, with a planting area of ​​354 million mu in 2023, distributed in various regions of my country. Common diseases of wheat include wheat rust, powdery mildew, fusarium head blight, sheath blight, root rot, downy mildew, and stem base rot.

[0003] Wheat rust can destroy the photosynthesis of the plant, causing a large amount of water loss in the wheat plant, reducing the plumpness and filling degree of the grains, causing premature aging of the leaves, and seriously reducing the yield and quality of wheat. Wheat rust is divided into three types: stripe rust, leaf rust and stem rust. When severe, stripe rust can reduce yield by 73% to 85%, leaf rust can reduce yield by 47% to 49%, and stem rust can reduce yield by 74% to 84%.

[0004] Wheat head blight is also known as wheat head blight, rotten wheat head, and red wheat head. The disease is caused by a variety of Fusarium, such as Fusarium graminearum, Fusarium avenae, Fusarium genus, and Fusarium moniliforme. The disease not only leads to a reduction in wheat production, but also affects the quality and edible value of wheat grains. Diseased wheat produces fungal toxins, mainly deoxynivalenol, which may cause acute poisoning symptoms such as dizziness, fever, and nausea in humans and animals after consumption.

[0005] Bixafen is a succinate dehydrogenase inhibitor (SDHI) fungicide developed by Bayer CropScience. Its mode of action is to inhibit succinate dehydrogenase (SDH) in complex II of the mitochondrial respiratory chain. It is a systemic, broad-spectrum fungicide and is widely used for foliar spraying. Studies have shown that bixafen has excellent control effects on many diseases of wheat crops, such as wheat leaf blight, leaf rust, stripe rust, eye spot and yellow spot, barley net spot, columnar septal leaf spot, moire and leaf rust, etc.; it can also effectively prevent and control corn leaf blight, gray leaf spot, brown spot and white mold; potato early blight and white mold; rapeseed white mold and peanut stem rot, leaf spot, leaf rust and Rhizoctonia; and can prevent and control leaf spot caused by Septoria pathogens that are resistant to strobilurins. Currently, clofazodone is registered and marketed in many countries and regions including the European Union, the United States, Canada, Brazil, Argentina, and Chile.

[0006] At present, the main chemical agents for the prevention and treatment of wheat rust and fusarium head blight are tebuconazole, diniconazole, hexaconazole, difenoconazole, prothiocarb, etc. With long-term application, the control effect of these agents on wheat diseases has gradually decreased. At the current stage, the development of new pesticides is difficult, costly, and time-consuming. Mixing registered fungicides is an efficient choice. After the fungicides are mixed, three different combined effects of synergism, addition, and antagonism appear. The synergistic fungicidal composition can effectively reduce the dosage of active ingredients, slow down the occurrence and development of bacterial resistance, and reduce environmental pollution. Therefore, many pesticide companies pay attention to the compounding of agents, hoping to screen out fungicidal compositions with excellent synergistic effects; at present, there are no reports in the prior art on the compounding of chlorfluanid and tebuconazole to achieve synergistic synergy. Summary of the invention

[0007] In order to solve the above problems, the present invention provides a fungicide composition, the ingredients of which include clofaxanil and tebuconazole.

[0008] Furthermore, the weight ratio of clofoxanil to tebuconazole is 1:1-50.

[0009] Further, the weight ratio of clofoxanil to tebuconazole is 16:34.

[0010] The present invention also provides a pesticide, the components of which include the above-mentioned fungicide composition.

[0011] Furthermore, the pesticide formulation is a suspension, a microemulsion or a water dispersible granule.

[0012] Furthermore, the pesticide formulation is a suspension concentrate.

[0013] The invention also provides application of the bactericidal composition in preventing and controlling wheat diseases.

[0014] Furthermore, the wheat diseases include wheat rust and wheat fusarium head blight.

[0015] The present invention has the following beneficial effects: 1. The composition of the present invention greatly reduces the amount of chemical pesticides used after compounding cyfluthrin and tebuconazole, and the control effect is much higher than that of a single agent, which has the effect of reducing costs and increasing efficiency.

[0016] 2. The composition of the present invention reduces pesticide residues and has little pollution to the environment. DETAILED DESCRIPTION

[0017] Now, various exemplary embodiments of the present invention are described in detail. The methods in the examples are conventional methods unless otherwise specified, and the reagents used are conventional commercially available reagents or reagents prepared by conventional methods unless otherwise specified. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features and embodiments of the present invention.

[0018] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0019] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0020] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0021] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0022] Example 1

[0023] In this example: a binary combination of clofoxanil and tebuconazole was subjected to indoor toxicity testing.

[0024] Test object: Wheat fusarium ( Fusarium graminearum ) Test basis: NY / T1156.2-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth", NY / T 1156.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 6 Determination of Combined Action of Mixtures".

[0025] Preparation: The active ingredients clofoxanil and tebuconazole were prepared into the required test preparation, and the single agent and each combination were set to at least 5 concentration gradients. Clear water was used as the control, and repeated 3 times.

[0026] Test method: Use the plate method. Cut the cultured pathogen from the edge of the colony with a sterile puncher with a diameter of 5 mm under sterile conditions, inoculate the cake with the inoculator in the center of the drug-containing plate, with the mycelium facing up, cover the plate, and place it in an incubator at a suitable temperature for cultivation. When the colony of the control group is full, measure the colony diameter and calculate the percentage of mycelium growth inhibition by each agent treatment.

[0027]

[0028] The EC50 values ​​of the two active ingredients as single agents and mixed agents were determined. The results were used to clarify the degree of synergy according to Sun Yunpei's co-toxicity coefficient (CTC) method, that is, CTC < 80 is antagonistic, 80 ≤ CTC ≤ 120 is additive, and CTC > 120 is synergistic. The higher the co-toxicity coefficient, the stronger the synergistic effect. The co-toxicity coefficient is calculated according to the following formula:

[0029]

[0030]

[0031]

[0032]

[0033] Where: is the relative toxicity index of fungicide A; is the relative toxicity index of fungicide B; is the measured toxicity index of the fungicide (A+B); It is the theoretical toxicity index of fungicide (A+B).

[0034] The indoor bioassay data were statistically analyzed using the probability value analysis method of DPS statistical software to obtain the toxicity regression equation and LD for each agent. 50 Value, 95% confidence limit, b value and related data were obtained, and the co-toxicity coefficient method was used to screen the synergistic formula.

[0035] Table 1 Indoor activity test of the active ingredients cypermethrin and tebuconazole against wheat scab pathogens Drug Name Virulence regression equation Correlation coefficient <![CDATA[Inhibitory medium concentration EC 50 (mg / L)]]> Measured toxicity index Theoretical toxicity index Co-toxicity coefficient A: Chlorofluanid y=6.9977+2.0213x 0.9992 0.1027 -- -- -- B: Tebuconazole y=5.5167+1.7314x 0.9974 0.5030 -- -- -- A:B=2:5 y=6.7950+1.9428x 0.9980 0.1192 86.16 43.16 199.65 A:B=8:17 y=7.0908+1.9916x 0.9997 0.0892 115.13 45.88 250.93 A:B=1:1 y=6.7759+1.7860x 0.9963 0.1013 101.38 60.21 168.38 A:B=6:5 y=6.6465+1.6617x 0.9964 0.1021 100.59 63.83 157.60 A:B=3:2 y=6.3737+1.4929x 0.9961 0.1202 85.44 68.17 125.37 From the indoor activity test (see Table 1), the EC values ​​of single-dose clofoxacin and tebuconazole against wheat fusarium head blight were 50 The co-toxicity coefficient (CTC) of clofoxanil and tebuconazole in the mass ratio range of 1.5-1:1-2.5 was greater than 120, showing a significant synergistic effect. The CTC value was the largest when clofoxanil:tebuconazole = 8:17, and the EC 50 It is 0.0892mg / L, and the effect is most significant.

[0036] Example 2 In this example: a binary combination of mefenamic acid and tebuconazole was subjected to indoor toxicity assay.

[0037] Test object: wheat rust Test basis: NY / T 1156.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides - Part 6 - Determination of the Combined Action of Mixtures".

[0038] Preparation of the agent: The original drug is prepared into the required test agent, and the test adopts the potting method. First, set more than 5 different concentration gradients for the single agent and each mixed agent. First, select wheat seeds with full grains, soak them in clean water for 1 hour, and then evenly sow them in a nutrient matrix filled with sandy loam. 25 seeds per pot, evenly sprinkle them on the surface with a covering soil matrix so that the seeds are completely covered, water them with enough water, keep them warm and moisturize them, and wait until they grow to 2-3 leaves for use. When determining the protective effect of the agent, spray the agent first. When the healthy wheat seedlings have 2 leaves, spray the agent on their leaves respectively. Set up a clear water control and a drug control. After 24 hours, inoculate wheat rust spores. After culturing in suitable dark conditions for 24 hours, move them to normal light moisturizing culture. One week after inoculation, when yellow spore piles appear on the leaves of the clear water control, investigate the disease index of each treated leaf separately, and take the average value of 3 repetitions as statistical data. By investigating the disease of wheat leaves, calculate its disease index and obtain the prevention effect of wheat rust. The grading standards are as follows: Level 0: no disease; Level 1: The lesion area accounts for less than 5% of the entire leaf area; Level 3: The lesion area accounts for 6% to 25% of the entire leaf area; Level 5: The lesion area accounts for 26% to 50% of the entire leaf area; Level 7: The lesion area accounts for 51% to 75% of the entire leaf area; Level 9: The lesion area accounts for more than 76% of the entire leaf area;

[0039]

[0040] The EC value of each drug was calculated by linear regression analysis between the probability value of the protective effect and the logarithmic value of the series concentration. 50 The value is calculated according to Sun Yunpei's co-toxicity coefficient (CTC) method to determine the degree of synergy, that is, CTC < 80 is antagonistic, 80 ≤ CTC ≤ 120 is additive, and CTC > 120 is synergistic. The higher the co-toxicity coefficient, the stronger the synergistic effect. The co-toxicity coefficient is calculated according to the following formula:

[0041]

[0042]

[0043]

[0044]

[0045] Where: is the relative toxicity index of fungicide A; is the relative toxicity index of fungicide B; is the measured toxicity index of the fungicide (A+B); It is the theoretical toxicity index of fungicide (A+B).

[0046] The indoor bioassay data were statistically analyzed using the probability value analysis method of DPS statistical software to obtain the toxicity regression equation and LD for each agent. 50 Value, 95% confidence limit, b value and related data were obtained, and the co-toxicity coefficient method was used to screen the synergistic formula.

[0047] Table 2 Indoor activity determination of the active ingredients cypermethrin and tebuconazole against wheat rust pathogens Drug Name Virulence regression equation Correlation coefficient <![CDATA[Inhibitory medium concentration EC 50 (mg / L)]]> Measured toxicity index Theoretical toxicity index Co-toxicity coefficient A: Chlorofluanid y=8.4876+3.1276x 0.9728 0.0767 -- -- -- B: Tebuconazole y=5.4707+1.7617x 0.9988 0.5405 -- -- -- A:B=2:5 y=8.5633+3.4705x 0.9863 0.0940 86.16 38.71 210.80 A:B=8:17 y=8.3330+2.8555x 0.9674 0.0680 112.79 41.65 270.82 A:B=1:1 y=7.9523+2.7325x 0.9894 0.0831 92.30 57.10 161.66 A:B=6:5 y=7.6960+2.6396x 0.9872 0.0952 80.57 61.00 132.09 A:B=3:2 y=7.8033+2.7030x 0.9753 0.0918 83.55 65.68 127.22 From the indoor activity test (see Table 2), the EC values ​​of single-dose cyfluthrin and tebuconazole against wheat rust were 50 The co-toxicity coefficient (CTC) of chlorofluorobipyraclostrobin and tebuconazole in the mass ratio range of 1.5-1:1-2.5 was greater than 120, showing a significant synergistic effect. The CTC value was the largest when chlorofluorobipyraclostrobin:tebuconazole = 8:17, and the EC 50 It is 0.0680mg / L, and the effect is most significant.

[0048] Example 3 Based on the above-mentioned ratio, this example carried out a field efficacy test of the composition in a wheat field in accordance with the pesticide field efficacy test guidelines.

[0049] Wheat fusarium head blight and wheat rust occurred in the field to varying degrees. Fertilizer, water and insect pests were managed as usual during the wheat growth period. The composition was applied once at the beginning of wheat heading and flowering, and then applied again after an interval of 6 days. The results were investigated at the milky stage. Each treatment was repeated three times, and the area of ​​each plot was 30 square meters. Each test plot was sampled at 5 diagonal points, and 100 to 200 ears were investigated at each point. The percentage of dead ear area to the entire ear area was used for classification, and the number of diseased ears at each level and the total number of ears were recorded. The disease classification standards are as follows: Level 0: no disease; Level 1: The lesion area accounts for less than 5% of the entire leaf area; Level 3: The lesion area accounts for 6% to 25% of the entire leaf area; Level 5: The lesion area accounts for 26% to 50% of the entire leaf area; Level 7: The lesion area accounts for 51% to 75% of the entire leaf area; Level 9: The lesion area accounts for more than 76% of the entire leaf area; Calculation method of drug efficacy: Based on the survey results, calculate the disease index and drug efficacy according to the following formula.

[0050]

[0051]

[0052] Table 3. Field efficacy test of active ingredients clofoxacin and tebuconazole against wheat scab and rust and safety test on wheat

[0053] *Different lowercase letters after the data in the same column indicate significant differences at the P<0.05 level.

[0054] The results of the field test show (see Table 3): the fungicide combination can effectively prevent and control wheat ergot and wheat rust, the control effect of the combination at the same dosage is significantly better than that of a single agent, and it is safe for the test crops within the test dosage range.

[0055] In summary, the results of indoor bioassay and field efficacy test show that the composition of the present invention has a synergistic effect and has excellent antibacterial and disease prevention effects on wheat rust and fusarium head blight. The composition is composed of active ingredients with different action mechanisms, which is conducive to overcoming and delaying the development of drug resistance of pathogens and is safe for test crops.

[0056] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A bactericidal composition, characterized in that: Ingredients include fluoxathiapiprolin and tebuconazole.

2. The bactericidal composition according to claim 1, characterized in that The weight ratio of clofoxacin to tebuconazole is 1:1-50.

3. The bactericidal composition according to claim 2, characterized in that The weight ratio of clofozolin and tebuconazole is 16:

34.

4. A pesticide, characterized in that: The composition comprises the bactericidal composition as claimed in claim 1.

5. The pesticide according to claim 4, characterized in that The pesticide formulation is a suspension, a microemulsion or a water-dispersible granule.

6. The pesticide according to claim 5, characterized in that The pesticide formulation is a suspension concentrate.

7. Use of the fungicide composition according to claim 1 in preventing and controlling wheat diseases.

8. The use according to claim 7, characterized in that: The wheat diseases include wheat rust and wheat fusarium head blight.

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