A fungicide composition for controlling grape powdery mildew

Abstract

This invention belongs to the field of pesticide technology, specifically relating to a fungicide composition for controlling grape powdery mildew. The active ingredient of this fungicide composition for controlling grape powdery mildew is a compound of fluopyram and dimethomorph or osthol. Through pot experiments, it was found that within a compounding mass ratio of 1-7:25-1, the combination of fluopyram and dimethomorph showed a synergistic effect on grape powdery mildew; within a compounding mass ratio of 1-19:19-1, the combination of fluopyram and osthol also showed a synergistic effect. This indicates that the compounding of fluopyram with dimethomorph or osthol can improve the control effect against grape powdery mildew, help reduce the dosage of pesticides, and delay the development of resistance, thus providing support for the development of novel pesticides for controlling grape powdery mildew.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a fungicide composition for controlling powdery mildew in grapes. Background Technology

[0002] Grape powdery mildew is a fungal disease caused by the obligate parasitic fungus *Uncinula necater*, which infects the green tissues of grapevines, including leaves, shoots, and bunches. When leaves are affected, pale green spots appear on the upper surface, while the veins on the lower surface turn black, and a powdery mold layer develops on both sides of the spots. In severe cases, the powdery mold layer spreads across the entire leaf surface, causing the leaves to wither and curl. When bunches are affected, irregular, pale green, grayish-white spots appear on the surface, later developing into yellowish-brown spots covered with a white powdery substance. When young fruit is affected, a pale green radial pattern appears on the surface, epidermal cells die, and a white powdery substance is present. Young fruit grows slowly and becomes deformed, rendering it inedible.

[0003] In the production process, the application of fungicides such as ethirimol, tebuconazole, hexaconazole, chlorothalonil, and thiophanate-methyl can control grape powdery mildew. However, the issue of fungicide resistance cannot be ignored when using fungicides to control the disease. Long-term use of a single fungicide can easily lead to the rapid accumulation and development of fungal resistance, resulting in reduced control efficacy. According to relevant reports, grape powdery mildew fungi have developed varying degrees of resistance to many existing fungicides, therefore, it is necessary to research new agents for the control of grape powdery mildew. Summary of the Invention

[0004] The purpose of this invention is to provide a fungicide composition for controlling grape powdery mildew, in order to solve the problem that grape powdery mildew fungi have developed varying degrees of resistance to a variety of existing fungicides.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A fungicide composition for controlling grape powdery mildew, wherein the active ingredient is a compound of fluopyram and diacetyl amide or osthol.

[0007] More specifically, the mass ratio of fluopyram to diacetylcholine is 1-7:25-1.

[0008] More specifically, the mass ratio of fluopyram aniline to osthol is 1-19:19-1.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] This invention combines fluopyram with dimethomorph or osthol. Pot experiments revealed that within a compounding ratio of 1-7:25-1, the combination of fluopyram and dimethomorph showed a synergistic effect against grape powdery mildew; within a compounding ratio of 1-19:19-1, the combination of fluopyram and osthol also showed a synergistic effect. This indicates that the combination of fluopyram with dimethomorph or osthol can improve the control efficacy against grape powdery mildew, help reduce the dosage of pesticides, and delay the development of resistance, thus providing support for the development of novel pesticides for controlling grape powdery mildew. Detailed Implementation

[0011] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0012] Example: Indoor toxicity test

[0013] 1. Test crop

[0014] One-year-old Kyoho grape seedlings, one seedling per pot, numbered for future use.

[0015] 2. Test spore suspension

[0016] The powdery mildew causal agent *Uncinula necater* was inoculated onto the test crops. After fresh spores were produced, the spores on the underside of the leaves were washed away with sterile water, filtered through double-layered gauze, and the spore concentration was adjusted to 5.0 × 10⁻⁶. 6 1 spore / mL, for later use.

[0017] 3. Test reagents

[0018] 96% fluopyram aniline technical grade (Inner Mongolia Zhonggao Chemical Co., Ltd.), 93% dimethomorph technical grade (Syngenta Crop Protection Ltd., Switzerland), 10% osthol technical grade (Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.)

[0019] The active ingredient was first dissolved in dimethyl sulfoxide and then diluted with 0.05% Tween-80 aqueous solution to prepare a single-dose stock solution; the Cnidium monnieri stock solution was directly diluted with 0.05% Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple formulations were set up, and each single-dose and formulation mixture was set up with 5 mass concentration gradients according to the proportional method.

[0020] 4. Test methods

[0021] The test spore suspension was evenly sprayed onto the leaves of the test crops. After 24 hours, the prepared pesticide solution was evenly sprayed on the leaves. Each concentration of pesticide solution was replicated five times, with three pots of test crops per replicate. A blank control was prepared by spraying an equal volume of 0.05% Tween-80 aqueous solution. After treatment, the potted plants were transferred to a culture environment of 25℃, 90% relative humidity, and 12L / 12D light. For both spore spraying and pesticide spraying, the solution should be sprayed until both sides of the leaves are wetted but not dripping.

[0022] After the blank control group has developed sufficient disease, the disease condition is investigated by grade. The disease grading method is shown in Table 1. The disease index is calculated and the control effect is calculated using the disease index.

[0023] Table 1 Disease Classification Method

[0024]

[0025] The disease index is calculated using the following formula:

[0026]

[0027] The disease prevention effect is calculated using the following formula:

[0028]

[0029] 5. Data Analysis

[0030] Using the logarithmic value of the pesticide concentration (x) as the independent variable and the probability value of the control effect (y) as the dependent variable, the toxicity regression equation and EC value of each pesticide were calculated. 50 The synergistic effect of the drug mixture was evaluated based on the correlation coefficient and the Wadley method, and the synergistic effect of the drug mixture was calculated according to the synergistic effect coefficient SR. The results are shown in Table 2-3.

[0031] Table 2. Combined Effect of Fluopyram and Dipyridamole on Grape Powdery Mildew

[0032]

[0033] Table 3. Combined Effect of Fluopyram and Osthol on Grape Powdery Mildew

[0034]

[0035] The experimental results in Tables 2 and 3 show that within the mass ratio range of 1-7:25-1, the combination of fluopyram and dimethomorph has a synergistic effect on grape powdery mildew; and within the mass ratio range of 1-19:19-1, the combination of fluopyram and osthol has a synergistic effect on grape powdery mildew.

[0036] In summary, the combination of fluopyram with dimethomorph or osthol in this invention can improve the control effect against grape powdery mildew, help reduce the dosage of the agent, and delay the development of resistance, thus providing support for the development of new agents for the control of grape powdery mildew.

[0037] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A fungicide composition for controlling grape powdery mildew, characterized in that, Its active ingredient is a compound of fluopyram and diacetyl amide; wherein the mass ratio of fluopyram to diacetyl amide is 1-7:25-1.

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