Compound composition for preventing and treating corn leaf spot disease and application thereof

Abstract

The invention discloses a compound composition for preventing and treating corn leaf spot disease and application thereof, and relates to the technical field of corn leaf spot disease prevention and treatment, and the compound composition comprises 2, 4-di-tert-butylphenol and surfactin. The invention finds a technology that the combination of the 2, 4-di-tert-butylphenol and the surfactin can antagonize the pathogenic bacteria related to the corn leaf spot disease, the combination of the 2, 4-di-tert-butylphenol and the surfactin greatly improves the control effect compared with the single use, and a theoretical basis is provided for the subsequent control of plant diseases and insect pests in the agricultural field.

Description

Technical Field

[0001] The invention relates to the technical field of corn leaf spot control, and more specifically to a composite composition for controlling corn leaf spot and application thereof. Background Art

[0002] Corn is an important food crop and feed crop in my country and even in the world. It is also the crop with the highest total output in the world. Its planting area is second only to rice and wheat. However, during the planting process of corn, it is often harmed by important diseases such as leaf spot. Corn leaf spot diseases are mainly fungal leaf spot diseases, and common ones include large leaf spot disease, small leaf spot disease, Curvularia leaf spot disease, gray leaf spot disease, Alternaria leaf spot disease and southern rust disease. The above diseases occur all year round or intermittently, causing significant losses to my country's corn production.

[0003] Leaf spot is a serious threat to corn production. In general years, the yield of diseased fields is reduced by about 10%, and in years when susceptible varieties are prevalent, the yield can be reduced by more than 50%, and even in severe cases, it can lead to a total crop failure. At present, the prevention and control of leaf spot is still mainly based on chemical control and disease-resistant varieties. However, due to the complexity of the pathogen, the large variation and differentiation of pathogenic bacteria, the diversity and differences of corn varieties themselves, and improper cultivation caused by climate and demand expansion, efficient and targeted chemical agents and disease-resistant varieties have not yet been obtained. While disease-resistant varieties cannot meet production needs, chemical control is also prone to problems such as pesticide residues and environmental pollution, and leads to the emergence of pathogen resistance.

[0004] Therefore, how to provide a composition with good effect in preventing and controlling corn leaf spot is a problem that needs to be solved urgently by those skilled in the art. Summary of the invention

[0005] In view of this, the present invention provides a composite composition for preventing and controlling corn leaf spot disease and application thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] One aspect of an embodiment of the present invention provides a composite composition for preventing and treating corn leaf spot disease, wherein the composite composition comprises 2,4-di-tert-butylphenol and surfactin.

[0008] Preferably, the concentration of 2,4-di-tert-butylphenol and surfactin is 9:1 to 1:9.

[0009] Preferably, the concentration of 2,4-di-tert-butylphenol and surfactin is 9:1.

[0010] The second aspect of the embodiment of the present invention provides the use of the composition in preparing a pharmaceutical preparation for preventing and treating corn leaf spot disease.

[0011] A third aspect of the embodiments of the present invention provides a pharmaceutical preparation for preventing and treating corn leaf spot, comprising the composition.

[0012] It can be seen from the above technical scheme that compared with the prior art, the present invention has discovered that the combination of 2,4-di-tert-butylphenol and surfactin can antagonize corn leaf spot-related pathogens. The combined use of the two greatly improves the prevention and control effect compared to the use of either alone, providing a theoretical basis for the subsequent prevention and control of pests and diseases in the agricultural field. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0014] Figure 1 The attached figure shows the inhibitory effect of the composite compound on 5 pathogens (7d); AE: pure culture of Echinococcus nigricans, Echinococcus kaoliang, Helminthosporium maydis, Alternaria alternata, Helminthosporium oris (CK); a1-e1: plates containing a composite compound concentration ratio of 2,4-di-tert-butylphenol:surfactin ratio of 9:1; a2-e2: plates containing a composite compound concentration ratio of 8:2; a3-e3: plates containing a composite compound concentration ratio of 7:3; a4-e4: plates containing a composite compound concentration ratio of 6:4; a5-e5: plates containing a composite compound concentration ratio of 5:5; a6-e6: plates containing a composite compound concentration ratio of 4:6; a7-e7: plates containing a composite compound concentration ratio of 3:7; a8-e8: plates containing a composite compound concentration ratio of 2:8; a9-e9: plates containing a composite compound concentration ratio of 1:9. DETAILED DESCRIPTION

[0015] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] Test materials:

[0017] The soil samples were collected from Wuli Village, Chong'an Street, Wuyishan City, Fujian Province (latitude: 27°75'116", longitude 118°3'86"). The five maize pathogens tested were provided by the Institute of Plant Protection, Fujian Academy of Agricultural Sciences, including Epicoccumnigrum, Micrococcus sorghum, Helminthodes panicularis, Bipolaris maydis, and Exserohilus m rostratum.

[0018] 2,4-di-tert-butylphenol and surfactin are both commercially available; surfactin was purchased from Shanghai MacLean Biochemical Technology Co., Ltd., S913578-1g, Lot#: C13441616, CAS: 24730-31-2.

[0019] Reagents and media

[0020] PDA medium (200 g of peeled potatoes, 20 g of glucose, 20 g of agar, 1 L of distilled water), PDB medium (200 g of peeled potatoes, 20 g of glucose, 1 L of distilled water), LB liquid medium (10 g of tryptone, 5 g of yeast extract, 10 g of NaCl).

[0021] Example 1

[0022] A composite composition for preventing and treating corn leaf spot disease, characterized in that the composition comprises 2,4-di-tert-butylphenol and surfactin in a concentration ratio of 1:9 to 9:1.

[0023] Example 2 Determination of the antibacterial effect of a single active organic compound

[0024] Five gradients of 2,4-di-tert-butylphenol and surfactin were set at 0.03 mg / mL, 0.05 mg / mL, 0.07 mg / mL, 0.09 mg / mL and 0.11 mg / mL. The compounds were added to PDA medium, dissolved and poured into plates. After solidification, pathogenic bacteria were inoculated. After constant temperature culture for 7 days, the inhibition rate was determined. See Table 1.

[0025]

[0026]

[0027] Table 1 Antibacterial rate of 2,4-di-tert-butylphenol against five pathogens of corn leaf spot

[0028] The results of the determination of the antibacterial rate of 2,4-di-tert-butylphenol against five corn leaf spot pathogens at different gradients are shown in Table 1. It can be seen that the antibacterial effect is positively correlated with the concentration of 2,4-di-tert-butylphenol added to the culture medium. When the concentration is 0.11 mg / mL, the inhibitory effect on pathogens is the best. Among them, the antibacterial effect on the five pathogens is more than 87%.

[0029] Table 2 Inhibition rate of surfactant on five corn leaf spot pathogens

[0030]

[0031] The results of the determination of the inhibition rate of surfactin on 5 kinds of corn leaf spot pathogens are shown in Table 2. It can be seen that surfactin has an inhibitory effect on different corn leaf spot pathogens, and its inhibitory effect is positively correlated with the concentration of surfactin added in the culture medium. When the concentration is 0.11 mg / mL, the inhibitory effect on pathogens is the best. Among them, the inhibitory effect on Zea mays is better, and the inhibition rate can reach up to 85.5%, but its inhibitory effect on Alternaria alternata is relatively weak.

[0032] Example 3 Determination of antibacterial effect of composite active organic compounds

[0033] Based on the antibacterial effects of individual compounds of 2,4-di-tert-butylphenol and surfactin, the two pure compounds were mixed in different ratios to make the final concentration ratio of the mixed drugs 2,4-di-tert-butylphenol: surfactin 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9, respectively, and the control was the pure culture CK1-5 of pathogens without adding any drugs. According to the concentration of the highest protective effect of the individual compound, it was configured according to the ratio. Add to PDA culture medium, dissolve and pour the plate, inoculate the pathogenic bacteria block after solidification, and culture at a constant temperature for 7 days. The results are as follows Figure 1 And as shown in Table 3.

[0034] like Figure 1 The pure products of 2,4-di-tert-butylphenol and surfactin were mixed in different proportions, added to PDA culture medium, poured into plates, and cultured at a constant temperature for 7 days after the inoculated bacteria blocks solidified. The pathogen pure culture AE was added to the control medium without adding compounds. It can be seen that the composite compound has different degrees of inhibitory effect on the five corn leaf spot pathogens at different ratios.

[0035] Table 3 Inhibition rate of composite active compounds against five maize leaf spot pathogens

[0036]

[0037]

[0038] The results of the determination of the inhibition rate of the compound of 2,4-di-tert-butylphenol and surfactin on five kinds of corn leaf spot pathogens are shown in Table 3. It can be seen that the compound compound has different degrees of inhibition effect on the five kinds of corn leaf spot pathogens at different concentration ratios, and its inhibition rate basically increases with the increase of the concentration of 2,4-di-tert-butylphenol in the compound ratio. When the ratio of the compound compound is 9:1 (2,4-di-tert-butylphenol: surfactin, the same below), the inhibition effect is the best. When the ratio of the compound compound is 4:6, the inhibition rate generally decreases significantly. When the ratio of the compound compound is 1:9, the inhibition rate is the lowest. Among them, the inhibition rate against Helicoverpa maydis is relatively stable.

[0039] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0040] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A composite composition for preventing and treating corn leaf spot, characterized in that: The composite composition comprises 2,4-di-tert-butylphenol and surfactin.

2. The composite composition for preventing and controlling corn leaf spot according to claim 1, characterized in that: The concentration of the 2,4-di-tert-butylphenol and surfactin is 9:1 to 1:

9.

3. A composite composition for preventing and controlling corn leaf spot according to claim 2, characterized in that: The concentration of 2,4-di-tert-butylphenol and surfactin is 9:

1.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of a pharmaceutical preparation for preventing and treating corn leaf spot.

5. A pharmaceutical preparation for preventing and treating corn leaf spot, characterized in that: The invention comprises the composition according to any one of claims 1 to 3.

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