Traditional Chinese medicine composition for preventing and treating chicken mycoplasma synoviae, preparation and preparation method

Abstract

The invention relates to the field of traditional Chinese veterinary medicines, and particularly discloses a traditional Chinese medicine composition for preventing and treating chicken mycoplasma synoviae, a preparation and a preparation method. The traditional Chinese medicine composition is prepared from the following raw material components in parts by mass: 10 to 25 parts of loofah sponge, 10 to 20 parts of radix cynanchi paniculati, 5 to 15 parts of semiliquidambar cathayensis, 4 to 10 parts of poria cocos, 4 to 10 parts of radix scutellariae, 1 to 10 parts of radix fici simplicissimae, 1 to 10 parts of pepper, 1 to 10 parts of radix astragali seu hedysari and 1 to 5 parts of borneol. According to the traditional Chinese medicine composition, loofah sponge, paniculate swallowwort root and semiliquidambar cathayensis serve as monarch drugs, poria cocos and scutellaria baicalensis serve as ministerial drugs, radix fici simplicissimae, pepper and borneol serve as adjuvant drugs, astragalus membranaceus serves as conductant drugs, and all the drugs are combined and matched to prepare the traditional Chinese medicine composition for preventing and treating the chicken mycoplasma synoviae disease. The traditional Chinese medicine composition is simple in formula and excellent in effect of preventing and treating the chicken mycoplasma synoviae, and the problems that in the prior art, a method for preventing and treating the chicken mycoplasma synoviae is poor in effect, and drug resistance is likely to be generated are effectively solved.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese veterinary medicines, and specifically discloses a traditional Chinese medicine composition, a preparation and a preparation method for preventing and treating chicken synoviae mycoplasma disease. Background Art

[0002] Mycoplasma gallinae is a prokaryotic organism that has no cell wall but a cell membrane. It is the smallest and simplest microorganism that can reproduce and synthesize its own macromolecules. Mycoplasma gallinae is one of the main pathogenic mycoplasmas of poultry. It can cause Mycoplasma synoviae infection in poultry such as chickens and turkeys. It may also cause mixed infection with avian influenza, Newcastle disease virus and Escherichia coli, leading to large-scale deaths of chickens and causing serious economic losses to the poultry farming industry.

[0003] When chickens are infected with Mycoplasma synoviae, they will develop exudative synovial bursitis and tenosynovitis. The clinical characteristics include lesions in the tarsal joints, foot pads and air sacs. After cutting open the swollen tarsal joints and foot pads, there will be yellow cheesy exudate or effusion. Most of the effusions are colorless, transparent or yellow, and in severe cases, bloody effusions may also appear. In joint pathological sections, desquamated synovial epithelial tissue can be observed in the joint cavity. The air sac lesions are mainly turbid, lose elasticity, and have yellow cheesy exudate. The combs and wattles are pale, accompanied by respiratory symptoms. In severe cases, there will be extreme emaciation, mental depression, lameness, and even paralysis, which will lead to a decrease in the egg production rate, egg quality and hatching rate of the chickens.

[0004] At present, the prevention and control methods of chicken synoviae mycoplasma disease mainly include environmental disinfection, antibiotic treatment, inactivated vaccine vaccination, etc. However, environmental disinfection can only prevent and cannot play a good therapeutic role, and although antibiotic treatment and vaccination have certain curative effects, they cannot completely eradicate the disease, and a large number of users develop drug resistance. Based on this, the development of a drug preparation that can effectively prevent and treat chicken synoviae mycoplasma disease and will not produce drug resistance is of great practical significance to the poultry farming industry. Summary of the invention

[0005] In view of the problem that the methods for preventing and treating chicken synovial bursal mycoplasma disease in the prior art are poor in effect and easy to produce drug resistance, the present invention provides a Chinese medicine composition, preparation and preparation method for preventing and treating chicken synovial bursal mycoplasma disease. The Chinese medicine composition uses luffa, Cynanchum cyrtonema and Radix jinlubanfenghe as monarch drugs, Poria cocos and Radix scutellariae as minister drugs, Prunus mume, Zanthoxylum bungeanum and Borneolum as adjuvant drugs, and Astragalus and as guiding drugs. Through the related effects of monarch, minister, adjuvant and guiding drugs, the Chinese medicine composition has the effects of promoting blood circulation, promoting diuresis and removing dampness, relaxing muscles and activating blood circulation, and has excellent effect on preventing and treating chicken synovial bursal mycoplasma disease.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following specific technical solutions: The first aspect of the present invention provides a traditional Chinese medicine composition for preventing and treating chicken synovia mycoplasma disease, comprising the following raw material components in parts by weight: 10-25 parts of loofah, 10-20 parts of cynanchum chinense, 5-15 parts of goldenseal, 4-10 parts of Poria, 4-10 parts of Scutellaria baicalensis, 1-10 parts of quinquefolius, 1-10 parts of Zanthoxylum bungeanum, 1-10 parts of Astragalus and 1-5 parts of borneol.

[0007] According to traditional Chinese medicine, Mycoplasma synoviae belongs to dampness and arthralgia according to the syndrome differentiation of traditional Chinese veterinary medicine. The causes of occurrence are mostly insufficient yang energy in the animal body, weak defensive qi, lying in wetlands for a long time, etc., and wind, cold and dampness take advantage of the opportunity to enter, injure the skin, flow through the meridians, and infringe on the muscles, resulting in meridian blockage and stagnation of qi and blood. Based on this, the present invention designs a Chinese medicine composition with loofah, Cynanchum chinense and Radix Glechomae as monarch drugs, Poria cocos and Radix Scutellariae as minister drugs, Prunus mume, Zanthoxylum bungeanum and Borneol as adjuvant drugs, and Astragalus and Radix Astragali as guiding drugs. The Chinese medicine composition has the effects of promoting blood circulation, promoting diuresis and removing dampness, relaxing muscles and activating blood circulation, and has excellent effects on preventing and treating Mycoplasma synoviae disease.

[0008] Luffa: It enters the lung, liver, and stomach meridians; it tastes sweet and is neutral in nature; it has the effects of promoting blood circulation, clearing away heat and resolving phlegm, cooling blood and detoxifying, and promoting diuresis and removing dampness.

[0009] Xu Changqing: enters the liver and stomach meridians; tastes spicy and is warm in nature; has the effects of calming and relieving pain, removing dampness and detoxifying, and inhibiting bacteria and anti-inflammatory.

[0010] Jinlu Banfenghe: enters the liver and spleen meridians; sweet in taste, warm in nature; has the effects of dispelling wind and dampness, relaxing muscles and activating blood circulation.

[0011] Poria: It enters the heart, lung, spleen and kidney meridians; it tastes sweet and mild, and is neutral in nature; it has the effects of promoting diuresis and eliminating dampness, strengthening the spleen and calming the mind.

[0012] Scutellaria baicalensis: It enters the lung, gallbladder, spleen, large intestine and small intestine meridians; it tastes bitter and is cold in nature; it has the effects of clearing away heat and dampness, purging fire and detoxifying.

[0013] Five-fingered ginseng: It enters the lung, spleen, stomach, liver and large intestine meridians; it tastes sweet and is slightly warm in nature; it has the effects of invigorating qi and strengthening the spleen, transforming dampness from the body, relaxing muscles and tendons, and removing phlegm and relieving asthma.

[0014] Zanthoxylum bungeanum: belongs to the lung, liver, kidney, spleen and heart meridians; it tastes spicy and is warm in nature; it has the effects of warming the middle and dispersing cold, removing dampness and relieving pain, relieving depression and killing insects.

[0015] Astragalus: It enters the lung and spleen meridians; it tastes sweet and is warm in nature; it has the effects of replenishing Qi and strengthening the exterior, promoting diuresis and expelling toxins, discharging pus, and healing sores and promoting tissue regeneration.

[0016] Borneol: enters the heart, spleen, and lung meridians; has a light fragrance, a pungent and bitter taste, and is cool in nature; has the effects of invigorating the mind, clearing away heat and relieving pain.

[0017] The present invention utilizes the drug relationship of monarch, minister, assistant and envoy, and after combining and matching the drug components, the diseased poultry can be quickly treated, and the curative effect is very significant. The present invention selects Chinese medicines with the functions of promoting blood circulation and activating collaterals, removing dampness and relieving pain, removing dampness and detoxifying, reducing swelling and relieving pain, and astringing sores and promoting tissue regeneration for combination, so as to achieve the purpose of effectively preventing and treating chicken synoviae mycoplasma disease. In addition, the pharmaceutical composition for preventing and treating chicken synoviae mycoplasma disease provided by the present invention has a simple formula and appropriate cost, and provides a new research idea for preventing and treating chicken synoviae mycoplasma disease.

[0018] Preferably, the traditional Chinese medicine composition for preventing and treating chicken synovia bursa mycoplasma disease comprises the following raw material components in parts by weight: 15-20 parts of luffa, 12-16 parts of Cynanchum wilfordii, 8-12 parts of Radix Achyranthis Bidentatae, 5-8 parts of Poria cocos, 5-8 parts of Scutellaria baicalensis, 3-7 parts of Prunus armeniaca, 3-8 parts of Zanthoxylum bungeanum, 2-8 parts of Astragalus membranaceus and 2-4 parts of Borneolum.

[0019] Further preferably, the traditional Chinese medicine composition for preventing and treating chicken synovia bursa mycoplasma disease comprises the following raw material components in parts by weight: 16-18 parts of luffa, 13-15 parts of Cynanchum wilfordii, 9-11 parts of Radix Angelicae Pubescentis, 6-7 parts of Poria, 6-7 parts of Scutellaria baicalensis, 4-6 parts of Prunus armeniaca, 4-7 parts of Zanthoxylum bungeanum, 3-6 parts of Astragalus and 3-4 parts of Borneolum.

[0020] The second aspect of the present invention provides a traditional Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease, wherein the traditional Chinese medicine preparation comprises the traditional Chinese medicine composition for preventing and treating chicken synoviae mycoplasma disease.

[0021] Preferably, the traditional Chinese medicine preparation also includes an emulsifier and a preservative.

[0022] Preferably, the dosage form of the traditional Chinese medicine preparation is any one of oral liquid, granules, tablets, spray or pills.

[0023] The third aspect of the present invention provides a method for preparing a Chinese medicine preparation for preventing and treating chicken synovia bursa mycoplasma disease, comprising the following steps: Step 1, weighing loofah according to the designed ratio, crushing, immersing in an alcohol solution, decocting 2-3 times, filtering, concentrating, cooling, and obtaining a first concentrated solution; Step 2, weighing the remaining raw material components except borneol and loofah according to the designed ratio, mixing them evenly, immersing them in deionized water, boiling them for 2-3 times, filtering, concentrating, and cooling to obtain a second concentrated solution; Step 3, dissolving borneol and an emulsifier in an alcohol solution, mixing evenly to obtain a borneol emulsion; evenly mixing the first concentrated solution, the second concentrated solution and the borneol emulsion, adding a preservative, adding water to make the drug-liquid ratio 0.9-1:1-1.1, sterilizing, and obtaining a traditional Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease.

[0024] Preferably, in step 1, the alcohol solution is a 35wt%-45wt% methanol solution.

[0025] Preferably, in step 1, the mass volume ratio of the loofah sponge to the alcohol solution is 1g:8mL-1g:12mL.

[0026] Preferably, in step 1, the immersion time is 6h-10h.

[0027] Preferably, in step 1, the concentration temperature is 95°C-120°C, and the concentration time is 30min-60min.

[0028] Preferably, in step one, the specific operation of decocting is to keep the mixture slightly boiling for 20-50 minutes after boiling.

[0029] Preferably, in step 1, the filtration is performed using a filter cloth with a mesh size of 80-120.

[0030] Preferably, in step 1, the concentration temperature is 90-110° C., and the concentration time is 20-50 min.

[0031] Preferably, in step 2, the mass volume ratio of the total mass of the remaining raw material components to the deionized water is 1g:10mL-1g:20mL.

[0032] Preferably, in step 2, the immersion time is 3h-6h.

[0033] Preferably, in step 2, the emulsifier is Tween 80.

[0034] Preferably, in step 2, the mass ratio of the emulsifier to borneol is 1:50-5:50.

[0035] Preferably, in step three, the preservative is sodium benzoate.

[0036] Preferably, in step three, the alcohol solution is a 40wt%-45wt% ethanol solution.

[0037] Preferably, in step three, the mass volume ratio of the borneol to the alcohol solution is 1g:10mL-1g:15mL.

[0038] Preferably, in step three, the amount of the preservative added is 0.3wt%-0.8wt% of the mass of the borneol emulsion.

[0039] Preferably, in step three, the sterilization temperature is 115°C-120°C, and the sterilization time is 25min-35min.

[0040] In summary, the present invention provides a Chinese medicine composition, preparation and preparation method for preventing and treating chicken synovial bursal mycoplasma disease. The Chinese medicine composition uses luffa, Cynanchum chinense and Radix Glechomae as monarch drugs, Poria cocos and Radix Scutellariae as minister drugs, Prunus mume, Zanthoxylum bungeanum and Borneolum as adjuvant drugs, and Astragalus and as messenger drugs. Through the related effects of monarch, minister, adjuvant and messenger, the Chinese medicine composition has the effects of promoting blood circulation, promoting diuresis and removing dampness, relaxing muscles and activating blood circulation, and has excellent effects on preventing and treating chicken synovial bursal mycoplasma disease. The technical solution of the present invention effectively solves the problem that the methods for preventing and treating chicken synovial bursal mycoplasma disease in the prior art are poor in effect and easy to produce drug resistance. DETAILED DESCRIPTION

[0041] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] Example 1 This embodiment provides a Chinese medicine composition and preparation for preventing and treating chicken synoviae mycoplasma disease, the specific contents are as follows: The traditional Chinese medicine composition for preventing and treating chicken synovia bursa mycoplasma disease comprises the following raw material components in parts by weight: 17g of loofah, 14g of Cynanchum chinense, 10g of Radix Achyranthis Bidentatae, 7g of Poria, 7g of Scutellaria baicalensis, 5g of Prunus armeniacae, 6g of Zanthoxylum bungeanum, 5g of Astragalus and 4g of Borneolum.

[0043] The preparation method of the traditional Chinese medicine preparation for preventing and treating chicken synovia bursa mycoplasma disease comprises the following steps: Step 1, weigh loofah according to the designed ratio, crush it, immerse it in 170mL of 40wt% methanol solution, boil it and continue to keep it slightly boiling for 30min, decoct it 3 times, filter it with 80-mesh filter cloth after decoction, concentrate the filtrate at 100°C for 40min, and cool it to obtain a first concentrated solution; Step 2: weigh the remaining raw material components except borneol and loofah according to the designed ratio, mix them evenly, immerse them in 1000 mL of deionized water, boil them and continue to keep them slightly boiling for 30 minutes, decoct them 3 times, filter them with 80-mesh filter cloth after decoction, concentrate the filtrate at 100° C. for 40 minutes, and cool them to obtain a second concentrated solution; Step 3, dissolving borneol and 0.2g Tween 80 in 60mL 40wt% ethanol solution, mixing evenly to obtain a borneol emulsion; mixing the first concentrated solution, the second concentrated solution and the borneol emulsion evenly, adding 0.28g sodium benzoate, adding water to make the drug-liquid ratio 1:1.1, sterilizing at 120°C for 30min, and obtaining a traditional Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease.

[0044] Example 2 This embodiment provides a Chinese medicine composition and preparation for preventing and treating chicken synoviae mycoplasma disease, the specific contents are as follows: The traditional Chinese medicine composition for preventing and treating chicken synovia bursa mycoplasma disease comprises the following raw material components in parts by weight: 18g of loofah, 15g of Cynanchum chinense, 9g of Radix Achyranthis Bidentatae, 6g of Poria, 7g of Radix Scutellariae, 6g of Prunus armeniacae, 4g of Zanthoxylum bungeanum, 5g of Radix Astragali and 4g of Borneolum.

[0045] The preparation method of the traditional Chinese medicine preparation for preventing and treating chicken synovia bursa mycoplasma disease comprises the following steps: Step 1, weigh loofah according to the designed ratio, crush it, immerse it in 170mL of 40wt% methanol solution, boil it and continue to keep it slightly boiling for 30min, decoct it 3 times, filter it with 80-mesh filter cloth after decoction, concentrate the filtrate at 100°C for 40min, and cool it to obtain a first concentrated solution; Step 2: weigh the remaining raw material components except borneol and loofah according to the designed ratio, mix them evenly, immerse them in 1000 mL of deionized water, boil them and continue to keep them slightly boiling for 30 minutes, decoct them 3 times, filter them with 80-mesh filter cloth after decoction, concentrate the filtrate at 100° C. for 40 minutes, and cool them to obtain a second concentrated solution; Step 3, dissolving borneol and 0.2g Tween 80 in 60mL 40wt% ethanol solution, mixing evenly to obtain a borneol emulsion; mixing the first concentrated solution, the second concentrated solution and the borneol emulsion evenly, adding 0.28g sodium benzoate, adding water to make the drug-liquid ratio 1:1, sterilizing at 125°C for 25min, and obtaining a traditional Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease.

[0046] Example 3 This embodiment provides a Chinese medicine composition and preparation for preventing and treating chicken synoviae mycoplasma disease, the specific contents are as follows: The traditional Chinese medicine composition for preventing and treating chicken synovia bursa mycoplasma disease comprises the following raw material components in parts by weight: 16g of loofah, 13g of Cynanchum chinense, 11g of Radix Achyranthis Bidentatae, 7g of Poria, 6g of Scutellaria baicalensis, 6g of Prunus armeniaca, 5g of Zanthoxylum bungeanum, 4g of Astragalus and 4g of Borneolum.

[0047] The preparation method of the traditional Chinese medicine preparation for preventing and treating chicken synovia bursa mycoplasma disease comprises the following steps: Step 1, weigh loofah according to the designed ratio, crush it, immerse it in 170mL of 40wt% methanol solution, boil it and continue to keep it slightly boiling for 30min, decoct it 3 times, filter it with 80-mesh filter cloth after decoction, concentrate the filtrate at 100°C for 40min, and cool it to obtain a first concentrated solution; Step 2: weigh the remaining raw material components except borneol and loofah according to the designed ratio, mix them evenly, immerse them in 1000 mL of deionized water, boil them and continue to keep them slightly boiling for 30 minutes, decoct them 3 times, filter them with a 100-mesh filter cloth after decoction, concentrate the filtrate at 100° C. for 40 minutes, and cool them to obtain a second concentrated solution; Step 3, dissolving borneol and 0.2g Tween 80 in 60mL 40wt% ethanol solution, mixing evenly to obtain a borneol emulsion; mixing the first concentrated solution, the second concentrated solution and the borneol emulsion evenly, adding 0.28g sodium benzoate, adding water to make the drug-liquid ratio 1:1, sterilizing at 120°C for 30min, and obtaining a traditional Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease.

[0048] Comparative Example 1 This comparative example provides a Chinese medicine composition and preparation for preventing and treating chicken synovia bursa mycoplasma disease. The difference from Example 1 is that loofah sponge is replaced with an equal amount of ephedra [1] clematis, and the other components and preparation methods remain unchanged and will not be described in detail here.

[0049] Comparative Example 2 This comparative example provides a Chinese medicine composition and preparation for preventing and treating chicken synovia bursa mycoplasma disease. The difference from Example 1 is that Cynanchum cyrtonema is replaced by an equal amount of Cinnamomum cassia twig and Saposhnikovia divaricata, and the other components and preparation methods remain unchanged, which will not be repeated here.

[0050] Comparative Example 3 This comparative example provides a traditional Chinese medicine composition and preparation for preventing and treating chicken synovia mycoplasma disease. The difference from Example 1 is that the Radix Angelicae Pubescentis is replaced with an equal amount of Inula fructus, and the other components and preparation methods remain unchanged and will not be described herein.

[0051] Test example This test example provides a Chinese medicine granule for preventing and treating chicken synoviae mycoplasma disease, using the Chinese medicine preparation provided in each embodiment or comparative example, specifically comprising the following steps: 30 g of medicinal starch, 0.5 g of sodium carboxymethyl cellulose and 0.3 g of β-cyclodextrin were added to 10 g of the traditional Chinese medicine preparation, mixed evenly and granulated to obtain traditional Chinese medicine granules for preventing and treating chicken synoviae mycoplasma disease.

[0052] To further illustrate the technical effect of the present invention, the present invention conducted a test on the treatment of chicken synoviae mycoplasma disease, and the specific contents are as follows: 1. Chicken flock treatment trial Experimental animals: Hy-Line Grey commercial laying hens, 65 days old.

[0053] Symptoms: decreased feed and water intake, pale combs and wattles, cysts in the sternum, swollen foot pads, lameness, weight loss, the chicken prefers to lie down and cannot get up, hot tarsal joints, and synovial bursae containing light yellow liquid or yellow jelly after cutting open the foot pads. Pathological sections of the joints show desquamated synovial epithelial tissue in the joint cavity.

[0054] Experimental groups: 700 broiler chickens with obvious and similar symptoms of synoviae Mycoplasma disease were selected from the above-mentioned sick chicken flocks and divided into group 1, group 2, group 3, group 4, group 5, group 6 and a control group, with 100 chickens in each group. Each group was fed the same feed, and the rest of the feeding and management were the same except for the different medications.

[0055] Experimental drugs: Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 1 of the present invention, Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 2 of the present invention, Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 3 of the present invention, Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 1 of the present invention, Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 2 of the present invention, and Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 3 of the present invention.

[0056] Test treatment: Group 1: Add the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 1 of the present invention into water at a ratio of 1 mL: 0.5 L, mix well, and give the water to sick chickens for drinking for 7 consecutive days; Group 2: Add the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 2 of the present invention into water at a ratio of 1 mL: 0.5 L, mix well, and give the water to sick chickens for drinking for 7 consecutive days; The third group: the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 3 of the present invention were added into water at a ratio of 1 mL: 0.5 L, mixed evenly, and given to sick chickens as drinking water for 7 consecutive days; Group 4: Add the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 1 of the present invention into water at a ratio of 1 mL: 0.5 L, mix well, and give the water to sick chickens for drinking for 7 consecutive days; The fifth group: the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 2 of the present invention were added into water at a ratio of 1 mL: 0.5 L, mixed evenly, and given to sick chickens for drinking for 7 consecutive days; Group 6: Add the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 2 of the present invention into water at a ratio of 1 ml: 0.5 L, mix well, and give the water to sick chickens for drinking for 7 consecutive days; Control group: no medication.

[0057] Observation of experimental symptoms: During the experiment, the disease progression of each group of experimental chickens was recorded, and the mental state, appetite, comb color and behavioral state of the sick chickens were observed.

[0058] Evaluation of therapeutic efficacy: Cure: After administration, the spirit, appetite and drinking water basically returned to normal, lameness, inability to get up from the ground and swollen joints were significantly improved, the chickens could walk normally, their feathers were smooth, their combs were a normal light red color, and the weight of a single chicken was ≥800g.

[0059] Effective: After administration, the spirit, appetite and drinking water have been significantly improved, lameness, inability to get up and joint swelling have been significantly improved, the chicken can walk normally, the feathers are smooth, the comb is a normal light red or light gray, and the weight of a single chicken is ≥700g.

[0060] Ineffectiveness: After medication, symptoms do not improve or even worsen.

[0061] The test results are shown in Table 1.

[0062] Efficacy evaluation: After administration, the cure rate and effective rate of the first, second, third, fourth, fifth and sixth groups were significantly higher than those of the control group, and the cure rate of the first, second and third groups was better than that of the fourth, fifth and sixth groups; the ineffective rate of the first, second, third, fourth, fifth and sixth groups was significantly lower than that of the control group, and the ineffective rate of the first, second and third groups was lower than that of the fourth, fifth and sixth groups. The specific data are shown in Table 1.

[0063] Table 1 Evaluation of the efficacy of chickens in each test group

[0064] 2. Chicken flock treatment trial Experimental animals: sick laying hens, 65 days old, raised in a chicken farm in Xingtai, Hebei.

[0065] Symptoms: A large group of laying hens experience decreased feed and water intake, pale combs and wattles, cysts in the sternum, swollen foot pads, lameness, emaciation, and the inability to get up from bed. The tarsal joints are hot and after cutting open the foot pads, the synovial bursa contains light yellow liquid or yellow jelly. When the joints of the sick chickens are dissected, desquamated synovial epithelial tissue can be observed in the joint cavity in the pathological sections.

[0066] Experimental grouping: The diseased chickens were divided into 7 groups, with 300 diseased chickens in each group. Each group was fed the same feed, and the rest of the feeding and management were the same except for the different medications.

[0067] Experimental drugs: Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 1 of the present invention, Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 2 of the present invention, Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 3 of the present invention, Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 1 of the present invention, Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 2 of the present invention, and Chinese herbal granules prepared from the Chinese herbal preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 3 of the present invention.

[0068] Test treatment: Group 1: Add the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 1 of the present invention into water at a ratio of 1 mL: 0.5 L, mix well, and give the water to sick chickens for drinking for 7 consecutive days; Group 2: Add the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 2 of the present invention into water at a ratio of 1 mL: 0.5 L, mix well, and give the water to sick chickens for drinking for 7 consecutive days; The third group: the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Example 3 of the present invention were added into water at a ratio of 1 mL: 0.5 L, mixed evenly, and given to sick chickens as drinking water for 7 consecutive days; Group 4: Add the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 1 of the present invention into water at a ratio of 1 mL: 0.5 L, mix well, and give the water to sick chickens for drinking for 7 consecutive days; The fifth group: the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 2 of the present invention were added into water at a ratio of 1 mL: 0.5 L, mixed evenly, and given to sick chickens for drinking for 7 consecutive days; Group 6: Add the Chinese medicine granules prepared from the Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease provided in Comparative Example 2 of the present invention into water at a ratio of 1 ml: 0.5 L, mix well, and give the water to sick chickens for drinking for 7 consecutive days; Control group: no medication.

[0069] Observation of experimental symptoms: During the experiment, the disease progression of each group of experimental chickens was recorded, and the mental state, appetite, comb color and behavioral state of the sick chickens were observed.

[0070] Evaluation of therapeutic efficacy: Cure: After administration, the spirit, appetite and drinking water basically returned to normal, lameness, inability to get up from the ground and swollen joints were significantly improved, the chickens could walk normally, their feathers were smooth, their combs were a normal light red color, and the weight of a single chicken was ≥800g.

[0071] Effective: After administration, the spirit, appetite and drinking water have been significantly improved, lameness, inability to get up and joint swelling have been significantly improved, the chicken can walk normally, the feathers are smooth, the comb is a normal light red or light gray, and the weight of a single chicken is ≥700g.

[0072] Ineffectiveness: After medication, symptoms do not improve or even worsen.

[0073] Test results: See Table 4 for details.

[0074] Efficacy evaluation: After administration, the cure rate and effective rate of the first, second, third, fourth, fifth and sixth groups were significantly higher than those of the control group, and the cure rate of the first, second and third groups was better than that of the fourth, fifth and sixth groups; the ineffective rate of the first, second, third, fourth, fifth and sixth groups was significantly lower than that of the control group, and the ineffective rate of the first, second and third groups was lower than that of the fourth, fifth and sixth groups. The specific data are shown in Table 2.

[0075] Table 2 Test results of each test group

[0076] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A Chinese medicine composition for preventing and treating chicken synoviae mycoplasma disease, characterized in that: The invention comprises the following raw material components in parts by weight: 10-25 parts of loofah, 10-20 parts of cynanchum chinense, 5-15 parts of golden thread herb, 4-10 parts of tuckahoe, 4-10 parts of scutellaria baicalensis, 1-10 parts of quinquefolius, 1-10 parts of zanthoxylum bungeanum, 1-10 parts of astragalus and 1-5 parts of borneol.

2. The Chinese medicinal composition for preventing and treating chicken synoviae mycoplasma disease according to claim 1, characterized in that: The invention comprises the following raw material components in parts by weight: 15-20 parts of loofah, 12-16 parts of radix scutellariae, 8-12 parts of herba scutellariae, 5-8 parts of tuckahoe, 5-8 parts of scutellariae, 3-7 parts of quinquefolius, 3-8 parts of zanthoxylum bungeanum, 2-8 parts of astragalus and 2-4 parts of borneol.

3. The Chinese medicinal composition for preventing and treating chicken synoviae mycoplasma disease according to any one of claims 1 or 2, characterized in that: The invention comprises the following raw material components in parts by weight: 16-18 parts of loofah, 13-15 parts of radix scutellariae, 9-11 parts of rhizoma jinlubanfenghe, 6-7 parts of tuckahoe, 6-7 parts of scutellariae, 4-6 parts of quinquefolius, 4-7 parts of zanthoxylum bungeanum, 3-6 parts of astragalus and 3-4 parts of borneol.

4. A Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease, characterized in that: A Chinese medicinal composition for preventing and treating chicken synoviae mycoplasma disease comprising any one of claims 1 to 3.

5. The Chinese medicinal preparation for preventing and treating chicken synoviae mycoplasma disease according to claim 4, characterized in that: The traditional Chinese medicine preparation also includes an emulsifier and a preservative.

6. The Chinese medicinal preparation for preventing and treating chicken synoviae mycoplasma disease according to claim 4 or 5, characterized in that: The dosage form of the traditional Chinese medicine preparation is any one of oral liquid, granules, tablets, sprays or pills.

7. A method for preparing the Chinese medicinal preparation for preventing and treating chicken synoviae mycoplasma disease as claimed in claim 5, characterized in that: The steps include: Step 1, weighing loofah according to the designed ratio, crushing, immersing in an alcohol solution, decocting 2-3 times, filtering, concentrating, cooling, and obtaining a first concentrated solution; Step 2, weighing the remaining raw material components except borneol and loofah according to the designed ratio, mixing them evenly, immersing them in deionized water, boiling them for 2-3 times, filtering, concentrating, and cooling to obtain a second concentrated solution; Step 3, dissolving borneol and an emulsifier in an alcohol solution, mixing evenly to obtain a borneol emulsion; evenly mixing the first concentrated solution, the second concentrated solution and the borneol emulsion, adding a preservative, adding water to make the drug-liquid ratio 0.9-1:1-1.1, sterilizing, and obtaining a traditional Chinese medicine preparation for preventing and treating chicken synoviae mycoplasma disease.

8. The method for preparing the Chinese medicinal preparation for preventing and treating chicken synoviae mycoplasma disease according to claim 7, characterized in that: In step 1, the alcohol solution is a 35wt%-45wt% methanol solution; and / or In step 1, the mass volume ratio of the loofah to the alcohol solution is 1g:8mL-1g:12mL; and / or In step 1, the immersion time is 6h-10h; and / or In step 1, the concentration temperature is 95° C.-120° C., and the concentration time is 30 min-60 min.

9. The method for preparing the Chinese medicinal preparation for preventing and treating chicken synoviae mycoplasma disease according to claim 7, characterized in that: In step 2, the mass volume ratio of the total mass of the remaining raw material components to the deionized water is 1g:10mL-1g:20mL; and / or In step 2, the immersion time is 3h-6h; and / or In step 2, the emulsifier is Tween 80; and / or In step 2, the mass ratio of the emulsifier to borneol is 1:50-5:50; and / or In step 3, the preservative is sodium benzoate; and / or In step 3, the alcohol solution is a 40wt%-45wt% ethanol solution; and / or In step 3, the mass volume ratio of the borneol to the alcohol solution is 1 g:10 mL-1 g:15 mL; and / or In step three, the amount of the preservative added is 0.3wt%-0.8wt% of the mass of the borneol emulsion.

10. The method for preparing the Chinese medicinal preparation for preventing and treating chicken synoviae mycoplasma disease according to claim 7, characterized in that: In step three, the sterilization temperature is 110°C-125°C, and the sterilization time is 20min-40min.

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