A negative pressure ultrafiltration centrifuge tube device

Abstract

This invention relates to the field of biofiltration technology, specifically disclosing a negative pressure ultrafiltration centrifuge tube device, comprising a negative pressure base, a negative pressure hood, and an ultrafiltration support stacked sequentially from bottom to top. The ultrafiltration support has multiple placement holes arranged in a matrix, each holding an ultrafiltration centrifuge tube. The negative pressure base contains a base frame, which has multiple sets of elastic limiting structures corresponding to the placement holes. These elastic limiting structures hold a collection tube, and the ultrafiltration centrifuge tube is coaxially arranged with its corresponding collection tube, with at least a portion of the ultrafiltration centrifuge tube extending axially into the collection tube. This invention enables liquid filtration, concentration, cell separation, desalination, and other experiments using negative pressure, and has a wide range of applications. Compared with existing technologies, this invention has the advantages of shorter processing time and higher efficiency, and it does not cause damage or leakage to the ultrafiltration membrane, effectively preventing cell injury.

Description

Technical Field

[0001] This invention relates to the field of biofiltration technology, and in particular to a negative pressure ultrafiltration centrifuge tube device. Background Technology

[0002] Ultrafiltration centrifuge tubes are disposable filtration devices that combine centrifugal force and ultrafiltration technology. In biological experiments, they provide reliable data for protein sample concentration, desalting, and buffer exchange. Compared to methods such as chromatography and dialysis, ultrafiltration centrifuge tubes process molecules more gently, do not require organic extraction, do not cause protein denaturation, and are fast, simple, and efficient. While separating molecules, they significantly increase the concentration factor and efficiency. Ultrafiltration is mainly used for desalting, percolation, or buffer replacement; concentration and purification of biological samples, including antigens, antibodies, enzymes, nucleic acids (single or dual-strain DNA / RNA samples), microorganisms, and eluates; purification of macromolecular components from tissue culture extracts or cell lysates; removal of primers, ligation molecules, or molecular markers from reaction mixtures; and protein removal before HPLC.

[0003] Existing ultrafiltration centrifuge tubes require centrifugation using a high-speed rotary centrifuge, which results in long centrifugation times, a limited number of tubes per centrifugation, and low efficiency. In addition, high-speed centrifugation can easily lead to damage and leakage of the ultrafiltration membrane, and may even cause abnormal phenomena such as cell injury and damage. Summary of the Invention

[0004] The technical problem to be solved by this invention is: how to solve the problems of low efficiency and easy damage and leakage of ultrafiltration membranes in the existing technology.

[0005] To solve the above-mentioned technical problems, the present invention provides a negative pressure ultrafiltration centrifuge tube device, comprising a negative pressure base, a negative pressure cover and an ultrafiltration support stacked and connected sequentially from bottom to top;

[0006] The ultrafiltration support has multiple placement holes arranged in a matrix, each containing an ultrafiltration centrifuge tube. A base frame is located within the negative pressure base, and the base frame has multiple sets of elastic limiting structures corresponding to the placement holes. These elastic limiting structures hold a collection tube. The ultrafiltration centrifuge tube and its corresponding collection tube are coaxially arranged, and at least a portion of the ultrafiltration centrifuge tube can extend axially into the collection tube. A first chamber is located on the side of the negative pressure base facing the ultrafiltration support. The first chamber contains at least one protrusion for supporting the base frame, configured to create a gap between the base frame and the negative pressure base. A sealing ring is embedded within each placement hole, and the ultrafiltration centrifuge tube can pass through the sealing ring. A first step structure is located on the side of the negative pressure cover near the ultrafiltration support, and a second step structure is located on the side of the ultrafiltration support near the negative pressure cover. The first and second step structures are coupled together.

[0007] More preferably, the sealing ring includes a first abutting surface and a second abutting surface, the first abutting surface and the second abutting surface respectively abutting the outer surface and the inner surface of the ultrafiltration support, and one end of the first abutting surface and the second abutting surface are connected to form an interlocking groove.

[0008] More preferably, the inner surface of the ultrafiltration support is provided with at least one first frame and at least one second frame, the first frame and the second frame being intersected.

[0009] More preferably, the negative pressure base has at least two protruding posts on the edge near the negative pressure cover, and the negative pressure cover has at least one cylindrical hole and at least one elliptical hole on the edge near the negative pressure base, with the at least two protruding posts respectively inserted into the cylindrical hole and the elliptical hole.

[0010] More preferably, the inner corners of the negative pressure cover are provided with hollowed-out rounded corners.

[0011] More preferably, a sealing sheet is provided between the first step structure and the second step structure.

[0012] More preferably, the thickness of the sealing sheet is 1-2 mm.

[0013] More preferably, the base frame has a second chamber on the side facing the ultrafiltration support, and multiple sets of elastic limiting structures are arranged in a matrix in the second chamber. Each set of elastic limiting structures includes at least two arc-shaped columns, which are placed on the same circumference to limit and clamp the collection tube.

[0014] More preferably, the base frame is further provided with reinforcing ribs, which are connected to the convex surface of the arc-shaped column.

[0015] More preferably, the base frame is provided with a through hole connecting the first chamber and the second chamber.

[0016] Compared with the prior art, the negative pressure ultrafiltration centrifuge tube device provided by this invention has the following advantages:

[0017] This invention utilizes a matrix arrangement of a negative pressure base, a negative pressure hood, and an ultrafiltration support, stacked sequentially from bottom to top. The placement holes allow for simultaneous filtration of multiple ultrafiltration centrifuge tubes, improving operational efficiency. Placing the ultrafiltration centrifuge tubes in the placement holes creates an independent, enclosed space within the negative pressure ultrafiltration centrifuge tube device. Under negative pressure, the collection tube can independently collect the solution from each ultrafiltration centrifuge tube, reducing potential differences in results and facilitating independent observation and testing. Furthermore, the elastic limiting structure on the base secures the collection tube to the base under negative pressure, preventing breakage or crushing of the collection tube during insertion or removal. The raised structure effectively... Raising the height of the base enhances internal gas flow and improves filtration efficiency. Under negative pressure, the sealing ring adheres well to the ultrafiltration support, ensuring a good seal. When the ultrafiltration centrifuge tube is removed, the sealing ring will not detach from the support. The first and second step structures make installation of the ultrafiltration support and negative pressure hood more convenient and flexible, preventing displacement under negative pressure. This invention enables liquid filtration, concentration, cell separation, desalination, and other experiments using negative pressure, offering a wide range of applications. Compared to existing centrifugal filtration methods using rotary high-speed centrifuges, this invention offers advantages such as shorter processing time and higher efficiency, and avoids damage to the ultrafiltration membrane, effectively preventing cell injury. Attached Figure Description

[0018] Figure 1 This is a perspective view of the negative pressure ultrafiltration centrifuge tube device described in this invention.

[0019] Figure 2 This is an exploded schematic diagram of the negative pressure ultrafiltration centrifuge tube device described in this invention.

[0020] Figure 3 This is a schematic diagram of the negative pressure base described in this invention.

[0021] Figure 4 This is a schematic diagram of the negative pressure cover described in this invention.

[0022] Figure 5 This is an assembly diagram of the negative pressure base and negative pressure cover described in this invention.

[0023] Figure 6 This is a schematic diagram of the structure of the ultrafiltration support described in this invention.

[0024] Figure 7 This is a structural schematic diagram of the ultrafiltration support described in this invention from another perspective.

[0025] Figure 8 This is a cross-sectional view of the ultrafiltration stent described in this invention.

[0026] Figure 9This is the present invention. Figure 8 Enlarged diagram of point A in the middle.

[0027] Figure 10 This is an exploded view of the negative pressure hood and ultrafiltration support described in this invention.

[0028] Figure 11 This is a schematic diagram of the assembly of the base frame and the collection pipe described in this invention.

[0029] Figure 12 This is an exploded schematic diagram of the base frame and collection pipe described in this invention.

[0030] Figure 13 This is a top view of the base frame described in this invention.

[0031] Figure 14 This is a top view of the negative pressure ultrafiltration centrifuge tube device described in this invention.

[0032] Figure 15 This is the present invention. Figure 14 A sectional view of section BB in the middle.

[0033] Figure 16 This is the present invention. Figure 14 A sectional view of section CC.

[0034] Figure label:

[0035] 10. Negative pressure base; 101. First chamber; 102. Connecting hole; 103. Protruding post; 104. Raised structure;

[0036] 20. Negative pressure cover; 201. Cylindrical hole; 202. Elliptical hole; 203. Hollowed-out rounded corner; 204. First step structure;

[0037] 30. Ultrafiltration support; 301. Placement hole; 302. Sealing ring; 302a. First abutment surface; 302b. Second abutment surface; 302c. Insertion groove; 303. First frame; 304. Second frame; 305. Second step structure;

[0038] 40. Ultrafiltration centrifuge tubes;

[0039] 50. Base frame; 501. Second chamber; 502. Arc-shaped column; 503. Through hole; 504. Reinforcing rib;

[0040] 60. Collection tube;

[0041] 70. Sealing sheet. Detailed Implementation

[0042] The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and are not intended to limit the scope of the invention.

[0043] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" used to indicate orientation or positional relationships are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0044] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0045] Furthermore, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0046] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0047] It should be noted that when an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0048] like Figures 1-16 As shown, this embodiment provides a negative pressure ultrafiltration centrifuge tube device, including a negative pressure base 10, a negative pressure hood 20, an ultrafiltration support 30, an ultrafiltration centrifuge tube 40, a base frame 50, and a collection tube 60. The negative pressure base 10 is connected to a negative pressure system. This embodiment can realize experiments such as liquid filtration, concentration, cell separation, and desalination through negative pressure.

[0049] In some embodiments, the negative pressure base 10, the negative pressure hood 20, and the ultrafiltration support 30 are stacked and connected sequentially from bottom to top, with the base frame 50 located inside the negative pressure base 10. The ultrafiltration support 30 is used to fix the ultrafiltration centrifuge tube 40, and the base frame 50 is used to fix the collection tube 60.

[0050] In some embodiments, the negative pressure base 10 has a first chamber 101 on the side facing the ultrafiltration support 30. The side wall of the negative pressure base 10 has a connection hole 102 communicating with the first chamber 101. The connection hole 102 is connected to the negative pressure system. When the negative pressure system is configured to start, it can make the first chamber 101 form a negative pressure state, thereby enabling experiments such as liquid filtration, concentration, cell separation, and desalination in the ultrafiltration centrifuge tube 40.

[0051] In some embodiments, the ultrafiltration support 30 has multiple placement holes 301 arranged in a matrix, and the ultrafiltration centrifuge tube 40 can be fixed axially in the placement holes 301, so as to meet the filtration use of multiple ultrafiltration centrifuge tubes 40 at the same time and improve the operating efficiency.

[0052] In some embodiments, the base frame 50 is provided with multiple sets of elastic limiting structures corresponding to the placement holes 301. The elastic limiting structures clamp the collection tube 60 and can fasten the collection tube 60 to the base frame 50 under negative pressure. At the same time, the elastic limiting structures will not break or cause the collection tube 60 to be crushed when the collection tube 60 is inserted or removed. The ultrafiltration centrifuge tube 40 is coaxially arranged with the corresponding collection tube 60, and at least a portion of the ultrafiltration centrifuge tube 40 can extend axially into the collection tube 60 so that the collection tube 60 can effectively collect the substances filtered out by the ultrafiltration centrifuge tube 40.

[0053] Compared to centrifugal filtration using a rotary high-speed centrifuge, this embodiment has the advantages of shorter time and higher efficiency, and will not cause damage or leakage to the ultrafiltration membrane, effectively preventing cell injury and damage.

[0054] In some embodiments, to ensure that the negative pressure ultrafiltration centrifuge tube device can form an independent, sealed space during operation, for example... Figures 6-9 As shown, a sealing ring 302 is embedded in the placement hole 301. The ultrafiltration centrifuge tube 40 can be inserted through the sealing ring 302, and the ultrafiltration centrifuge tube 40 and the sealing ring 302 are interference-fitted. Thus, when the ultrafiltration centrifuge tube 40 is inserted through the sealing ring 302, the placement hole 301 can be completely sealed, preventing external gas from entering the interior and affecting the experimental results.

[0055] In other embodiments, the sealing ring 302 is preferably a medical-grade silicone ring to avoid contaminating the specimen.

[0056] In addition, the sealing ring 302 can undergo elastic deformation during the insertion and removal process without damaging the ultrafiltration centrifuge tube 40, thus avoiding damage caused by friction between the ultrafiltration centrifuge tube 40 and the ultrafiltration support 30 during the insertion and removal process. Furthermore, the sealing ring 302 can be interference-fitted with the ultrafiltration centrifuge tube 40, and the sealing ring 302 tightly holds the ultrafiltration centrifuge tube 40, further improving the reliability of the experimental results.

[0057] In some embodiments, the sealing ring 302 includes a first abutting surface 302a and a second abutting surface 302b, which abut against the outer surface and inner surface of the ultrafiltration support 30, respectively. One end of the first abutting surface 302a and the second abutting surface 302b are connected to form an interlocking groove 302c. Thus, the sealing ring 302 adopts an embedded structure, which is more robust and durable. When the inside of the negative pressure ultrafiltration centrifuge tube device is in a negative pressure state, the pressure on the outer side of the sealing ring 302 is greater than the pressure on the inner side. At this time, the first abutting surface 302a fits against the outer surface of the ultrafiltration support 30 to form a good sealing effect. When the ultrafiltration centrifuge tube 40 is pulled out, the second abutting surface 302b fits against the inner surface of the ultrafiltration support 30, which limits the sealing ring 302. The sealing ring 302 will not detach from the ultrafiltration support 30 along with the ultrafiltration centrifuge tube 40. The ultrafiltration centrifuge tube 40 can be replaced to perform negative pressure filtration again without replacing the sealing ring 302, which is convenient and quick.

[0058] In some embodiments, the inner surface of the ultrafiltration support 30 is further provided with at least one first skeleton 303 and at least one second skeleton 304, with the first skeleton 303 and the second skeleton 304 intersecting each other. By providing the first skeleton 303 and the second skeleton 304, the structural strength of the ultrafiltration support 30 can be effectively enhanced. On the one hand, the ultrafiltration support 30 will not deform under negative pressure, and on the other hand, the ultrafiltration support 30 will not be damaged when inserting or removing the ultrafiltration centrifuge tube 40.

[0059] In other embodiments, the first frame 303 and the second frame 304 intersect perpendicularly.

[0060] In other embodiments, the ultrafiltration support 30 is an aluminum alloy support, which, together with the skeleton, can improve the structural strength of the ultrafiltration support 30.

[0061] In some implementations, such as Figure 3 As shown, the first chamber 101 of the negative pressure base 10 is provided with at least one protrusion 104 for supporting the base frame 50. The protrusion 104 is configured to form a gap between the base frame 50 and the negative pressure base 10, which can effectively raise the height of the base frame 50, enhance the internal gas flow and improve the filtration effect.

[0062] In some embodiments, the base frame 50 is provided with a second chamber 501 on the side facing the ultrafiltration support 30, and the base frame 50 is provided with a through hole 503 connecting the first chamber 101 and the second chamber 501, so as to more effectively form air pressure flow and further enhance the internal gas flow.

[0063] In some implementations, to further improve the smoothness of air circulation, the inner corners of the negative pressure cover 20 are provided with hollowed-out rounded corners 203.

[0064] In some implementations, such as Figures 3-5 As shown, the negative pressure base 10 has at least two protrusions 103 on the edge near the negative pressure cover 20, and the negative pressure cover 20 has at least one cylindrical hole 201 and at least one elliptical hole 202 on the edge near the negative pressure base 10. The at least two protrusions 103 are respectively inserted into the cylindrical hole 201 and the elliptical hole 202, so as to achieve precise insertion of the negative pressure base 10 and the negative pressure cover 20, and to limit the rotational joint between the negative pressure base 10 and the negative pressure cover 20.

[0065] Specifically, in this embodiment, two protrusions 103 are preferably provided on opposite sides of the negative pressure base 10. The cylindrical hole 201 and the elliptical hole 202 are also provided on opposite sides of the negative pressure cover 20. When the negative pressure base 10 and the negative pressure cover 20 are connected, one of the protrusions 103 is inserted into the cylindrical hole 201, and the other protrusion 103 is inserted into the elliptical hole 202. The setting of the elliptical hole 202 allows the negative pressure cover 20 to adapt to the negative pressure base 10 more flexibly, thereby improving assembly efficiency.

[0066] In some implementations, such as Figure 10As shown, the negative pressure hood 20 has a first step structure 204 on the side near the ultrafiltration support 30, and the ultrafiltration support 30 has a second step structure 305 on the side near the negative pressure hood 20. The first step structure 204 and the second step structure 305 are coupled to each other. By setting the first step structure 204 and the second step structure 305, the ultrafiltration support 30 and the negative pressure hood 20 are more convenient and flexible to install, and can remain in a negative pressure state without shifting.

[0067] In some embodiments, to further improve the airtightness of the connection between the negative pressure hood 20 and the ultrafiltration support 30, a sealing sheet 70 is provided between the first step structure 204 and the second step structure 305. Thus, under negative pressure, the ultrafiltration support 30 is subjected to a downward force, and the first step structure 204 and the second step structure 305 are tightly connected under the action of the sealing sheet 70 to form a sealed state.

[0068] In other embodiments, the sealing sheet 70 is preferably a medical-grade silicone sheet to avoid contaminating the specimen.

[0069] In some embodiments, the thickness of the sealing sheet 70 is 1-2 mm, preferably 1.5 mm.

[0070] In some embodiments, the negative pressure cover 20 is an acrylic glass negative pressure cover, which has the advantages of transparency and toughness, making it convenient for observation and improving the fit between the negative pressure cover 20 and the ultrafiltration support 30.

[0071] In some implementations, such as Figures 11-13 As shown, the base frame 50 has a second chamber 501 on the side facing the ultrafiltration support 30. Multiple sets of elastic limiting structures are arranged in a matrix in the second chamber 501. Each set of elastic limiting structures can independently fix a collection tube 60 to prevent the collection tube 60 from shifting. At the same time, it can reduce the possible differences in the results caused by each collection tube 60, and facilitate independent observation and inspection.

[0072] In some embodiments, each set of elastic limiting structures includes at least two arc-shaped pillars 502. In this embodiment, three arc-shaped pillars 502 are preferred. The three arc-shaped pillars 502 are placed on the same circumference. The three arc-shaped pillars 502 surround and limit the collection tube 60 so that the collection tube 60 can be fastened to the base frame 50 under negative pressure.

[0073] In some embodiments, in order to effectively prevent the arc-shaped column 502 from breaking or the collection tube 60 from being squeezed when inserting or removing the collection tube 60, the base frame 50 is also provided with a reinforcing rib 504. The reinforcing rib 504 is connected to the convex surface of the arc-shaped column 502, thereby enhancing the structural strength of the arc-shaped column 502.

[0074] The working process of this invention is as follows: Please combine Figures 1-16In use, first fix the collection tube 60 on the elastic limiting structure of the base frame 50, then place the base frame 50 on the negative pressure base 10, insert the cylindrical hole 201 and elliptical hole 202 of the negative pressure cover 20 into the protrusions 103 at both ends of the negative pressure base 10 respectively, pass the ultrafiltration centrifuge tube 40 through the sealing ring 302, connect the second step structure 305 of the ultrafiltration support 30 to the first step structure 204 of the negative pressure cover 20, adjust the position of the ultrafiltration centrifuge tube 40 so that at least part of it can extend axially into the collection tube 60, connect the external negative pressure system to the connection hole 102, and after starting the negative pressure system, the substance in the ultrafiltration centrifuge tube 40 can achieve experiments such as liquid filtration, concentration, cell separation, and desalination under negative pressure.

[0075] In summary, the negative pressure ultrafiltration centrifuge tube device provided by this invention can realize experiments such as liquid filtration, concentration, cell separation, and desalination through negative pressure. It has a wide range of applications. Compared with the existing centrifugal filtration method using a rotary high-speed centrifuge, this invention has the advantages of short time and high efficiency, and will not cause damage or leakage to the ultrafiltration membrane, effectively avoiding cell injury and damage.

[0076] The above description is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art can make various improvements and substitutions without departing from the technical principles of the present invention, and these improvements and substitutions should also be considered within the scope of protection of the present invention. The basic principles, main features, and advantages of the present invention have been shown and described above. It is obvious to those skilled in the art that the present invention is not limited to the details of the above preferred embodiments. The embodiments should be considered exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the foregoing description. Therefore, it is intended that all changes falling within the meaning and scope of the equivalents of the claims be included within the present invention.

[0077] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in the embodiments can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A negative pressure ultrafiltration centrifuge tube device, characterized in that, It includes a negative pressure base, a negative pressure cover, and an ultrafiltration support, which are stacked and connected from bottom to top. The ultrafiltration support has multiple placement holes arranged in a matrix. Each placement hole holds an ultrafiltration centrifuge tube. A base frame is located within the negative pressure base. The base frame has multiple sets of elastic limiting structures corresponding to the placement holes. These elastic limiting structures hold a collection tube. The ultrafiltration centrifuge tube and its corresponding collection tube are coaxially arranged, and at least a portion of the ultrafiltration centrifuge tube can extend axially into the collection tube. A first chamber is located on the side of the negative pressure base facing the ultrafiltration support. The first chamber contains at least one protrusion for supporting the base frame. The protrusion is configured to create a gap between the base frame and the negative pressure base. A sealing ring is embedded in each placement hole, and the ultrafiltration centrifuge tube can pass through the sealing ring. A first step structure is located on the side of the negative pressure cover near the ultrafiltration support, and a second step structure is located on the side of the ultrafiltration support near the negative pressure cover. The first and second step structures are coupled together. The base frame has a second chamber on the side facing the ultrafiltration support. Multiple sets of elastic limiting structures are arranged in a matrix in the second chamber. Each set of elastic limiting structures includes at least two arc-shaped columns, which are placed on the same circumference to limit and clamp the collection tube.

2. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that, The sealing ring includes a first abutting surface and a second abutting surface, which abut against the outer surface and inner surface of the ultrafiltration support, respectively, and one end of the first abutting surface and the second abutting surface are connected to form an interlocking groove.

3. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that, The inner surface of the ultrafiltration support is also provided with at least one first frame and at least one second frame, which are arranged to intersect.

4. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that, The negative pressure base has at least two protruding posts on the edge near the negative pressure cover, and the negative pressure cover has at least one cylindrical hole and at least one elliptical hole on the edge near the negative pressure base. The at least two protruding posts are respectively inserted into the cylindrical hole and the elliptical hole.

5. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that, The negative pressure cover has hollowed-out rounded corners at its inner corners.

6. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that, A sealing sheet is provided between the first step structure and the second step structure.

7. The negative pressure ultrafiltration centrifuge tube device according to claim 6, characterized in that, The thickness of the sealing sheet is 1-2 mm.

8. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that, The base frame is also provided with reinforcing ribs, which are connected to the convex surface of the arc-shaped column.

9. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that, The base frame is provided with a through hole connecting the first chamber and the second chamber.

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