New strain of Dictyophora alba and its domestication and cultivation method

Abstract

The present application discloses a new strain of Bamboo Fungus ochraceus and a method for its domestication and cultivation, and relates to the cultivation of Bamboo Fungus ochraceus, which is Bamboo Fungus ochraceus ZJZS007 strain, and is classified and named Phallus cremeo‑ ochre The deposit certificate number is CCTCC M 20242246. The strain ZJZS007 of Dictyophora alba is suitable for artificial cultivation and can realize large-scale production of this variety of Dictyophora alba.

Description

Technical Field

[0001] The present application relates to the technical field of Bamboo Fungus dahliae cultivation, in particular to a new Bamboo Fungus dahliae strain and a domestication and cultivation method thereof. Background Art

[0002] Phallus Phallus spp. ) is a grass-rotting fungus, a soil-covered cultivation type fungus, with obvious fungus skirts, so "Duck Fungus" is another name for the genus Psoralea. Duck Fungus is rich in nutrients, has a sweet and strong smell, and a unique flavor. It has good health and medicinal value, and has antibacterial, anti-cancer, blood pressure, blood lipid, cholesterol, tumor inhibition, and immune function regulation effects. It enjoys the reputation of "Queen of Fungi", "Flower of Fungi", "Flower of Mountain Treasures", and "Fairy of Snow Skirt".

[0003] So far, there are 12 species of bamboo fungus (with skirt or sheath) that are cultivated on a large scale and produced in my country, most of which are edible, such as Dictyophora malus ( P. haitangensis )、Dictyophora serrata( P. serratus )、Mini Skirt Bamboo Fungus( P. over-doubled )、Dictyophora spinulosa( P. echinovolvatus )、Pure Dictyophora japonica( P. luteus ) and Dictyophora rubra ( P. red-faced ), Dictyophora spinulosa and Dictyophora rubra have been widely cultivated on a large scale; there are also some wild species that are often eaten as the above species, including Dictyophora rubra ( P. creamy-ochreous )、Golden Dictyophora ( P. golden )、Darkthorn Bamboo Fungus( P. fuscoechinovolvatus )wait.

[0004] At present, "Zhijin Bamboo Sun" (Red Tuo Bamboo Sun) and "Dafang Winter Sun" (Winter Sun) have obtained the national geographical indication product certification and have been listed as the key development varieties of the edible fungi industry in Guizhou Province. The cultivation industry has achieved rapid development and even become a pillar industry of the local economy, driving the fight against poverty.

[0005] In 2020, the planting scale of red stem bamboo fungus reached 80 million sticks, with obvious economic benefits. However, other bamboo fungi, such as white ochre bamboo fungus ( P. creamy-ochreous )、Pure Dictyophora japonica( P. luteus )、Darkthorn Bamboo Fungus( P.fuscoechinovolvatus ) and Dictyophora chinensis ( P. lutescens ) have not yet been successfully domesticated and cultivated, and still have huge development potential.

[0006] In the existing technology, only domestic experts in large-scale fungi classification and identification (Li Ting, Li Yanzhen, Li Taihui, et al. Correction of the trade names of several commercially available bamboo fungi in my country [J]. Journal of Edible Fungi, 2023, 30(5):94-102. DOI:10.16488 / j.cnki.1005-9873.2023.05.010.) disclosed the morphological characteristics, habitat and distribution, spore characteristics, and type specimens collected from Xiaoqikong, Libo, Guizhou. However, they did not mention how to achieve artificial cultivation of white ochre bamboo fungus, nor did they mention which white ochre bamboo fungus strain has higher crude polysaccharide, total sugar, and selenium content than other existing bamboo fungus species.

[0007] Therefore, the prior art lacks a method for realizing artificial and large-scale planting of Dictyophora alba and a schematic strain thereof.

[0008] The information disclosed in the background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0009] In view of the above technical problems, the present application provides a new strain of Phallus cremeo-ochraceus and a domestication and cultivation method thereof. The strain is Phallus cremeo-ochraceus strain ZJZS007, which is suitable for artificial large-scale cultivation.

[0010] The present application provides a new strain of Bambusa ochracea, CCTCC NO: M20242246.

[0011] Another aspect of the present application also provides a cultivation method for a new strain of Bamboo Spatholobi, comprising the following steps:

[0012] After the test tube strain of the above-mentioned Bamboo Fungus ZJZS007 is propagated by mother strain and liquid strain, the obtained liquid strain is inoculated on the cultivation material and cultivated to the cultivation material;

[0013] Cover the cultivation material covered with mycelium with soil to stimulate the formation of primordium until primordium appears. Continue to cultivate the fungus until the buds of the white ochre bamboo fungus break and the fungus skirt is spread out, then it can be harvested.

[0014] Preferably, the mother strain propagation operation comprises the following steps: inoculating the test tube strain of Bamboo Spatholobi ZJZS007 on the mother strain culture medium, culturing for 13 to 18 days at a temperature range of 20 to 25° C. and in dark conditions to obtain the mother strain of Bamboo Spatholobi ZJZS007.

[0015] Preferably, the mother culture medium is composed of 18-20 g glucose, 2-3 g peptone, 2-3 g yeast powder, 1-2 g potassium dihydrogen phosphate, 1-2 g magnesium sulfate, 15-20 g agar, and 1 L of water.

[0016] Preferably, the liquid strain propagation operation comprises the following steps: the mother strain of Bamboo Fungus Root ZJZS007 is inoculated into a liquid culture medium, and cultured at a temperature of 22-25° C. in the dark for 6-10 days to obtain the liquid strain of Bamboo Fungus Root ZJZS007.

[0017] Preferably, the composition of the liquid culture medium is: 18-20 g glucose, 2-3 g peptone, 2-3 g yeast powder, 1-2 g potassium dihydrogen phosphate and 1-2 g magnesium sulfate and 1 L of water.

[0018] Preferably, the inoculation amount of the liquid bacteria inoculated into the culture medium is 30-45 mL / bag.

[0019] Preferably, the cultivation conditions on the culture medium are cultivation at a temperature of 20-25° C. and in darkness.

[0020] Preferably, the cultivation material is filled into a material bag and then inoculated with liquid bacteria; the material bag used is a polypropylene plastic bag.

[0021] Preferably, the preparation method of the cultivation material is as follows: 54-63 parts by weight of bamboo chips, 26-30 parts by weight of corn cobs, 10-15 parts by weight of wheat bran, and 1 part by weight of gypsum are weighed;

[0022] Soak bamboo chips and corn cobs for 12 to 24 hours and then mix them with wheat bran and gypsum to obtain a cultivation material with a water content of 60% to 65%.

[0023] Preferably, the cultivation environment is controlled after covering the soil: the air humidity is between 50% and 90%, the temperature is between 15°C and 28°C, the light intensity is 200 to 2000LX, and ventilation is required in time when the temperature is higher than 30°C.

[0024] Preferably, after the hyphae in the fungus bag are twisted and crawl out of the soil surface, heavy water is sprayed at least once by drip irrigation or sprinkler irrigation to irrigate the covering soil material to stimulate the formation of primordium.

[0025] The beneficial effects of this application include:

[0026] 1) The new strain of Dictyophora alba provided in this application, Dictyophora alba ZJZS007 strain, is suitable for artificial cultivation and can realize large-scale production of this variety of Dictyophora alba.

[0027] 2) The new strain of Bamboo Fungus spatholobi provided in this application and its domestication and cultivation method, Bamboo Fungus spatholobi ZJZS007 has a larger fruiting body, an average stipe length of (22.58±0.83) cm, and an average diameter of (3.25±0.14) cm; the fungus skirt droops to the fungus peduncle (i.e., the length of the fungus skirt is basically the same as the length of the fungus peduncle), is firm, and the net fungus skirt is composed of polygonal grids with a diameter of 0.5~2.1 cm and a thickness of 0.2~0.4 cm. After drying, it is resistant to storage and transportation and has good commercial properties. The bamboo fungus products produced by Bamboo Fungus spatholobi ZJZS007 are obviously different from the existing Bamboo Fungus spatholobi and Bamboo Fungus spatholobi of the same genus of edible fungi, and its fungus eggs are white to light pink, the fruiting body is large, the fungus skirt is long and thick, and the taste is crisp.

[0028] 3) The new strain of Bamboo Fungus ochre provided in this application and its domestication and cultivation method are prepared by inoculating the liquid strain into the cultivation material bag, and the average growth rate of the bag is 1.86 mm·d -1 , the growth rate is faster than the existing Zhongjun Zhusun No. 1 and Qianfeng No. 1, the full bag time is the shortest, only 81 days, the mycelium grows well, can effectively resist the growth of foreign bacteria, and the mycelium morphology is dense, thick, and grows evenly. After artificial cultivation, the strain has good consistency in strain and age, strong resistance to foreign bacteria, can effectively shorten the strain cultivation time, reduce costs, and simplify the process. The crude polysaccharide (7.63g / 100g), total sugar (42%), and selenium content (1.07mg / kg) of this strain are higher than those of the existing Zhongjun Zhusun No. 1 and Qianfeng No. 1.

[0029] Collection information:

[0030] Bamboo fungus (Classification and nomenclature: Creamy-ochreous phallus ) ZJZS007 has been deposited in the China Center for Type Culture Collection, address: Wuhan University, Wuhan, China; Postal Code: 430072, its deposit date is October 17, 2024, and the deposit number is CCTCC NO: M20242246; the white ochre bamboo strychnos ZJZS007 strain belongs to the Basidiomycetes, Agaricomycetes, Phalloides, Phallaceae, and Phallus. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 A photo of wild Bamboo eggs of Dictyophora alba collected in Example 1 provided in this application;

[0032] Figure 2 This is a photo of the fruiting body of Bambusa ochracea ZJZS007 domesticated and cultivated in Example 1 provided in this application;

[0033] Figure 3 This is a photo of the mycelium growth of Bamboo Fungus ZJZS007 cultured on a plate for 19 days in Example 1 provided in this application;

[0034] Figure 4This is a microscopic photograph of the mycelium of Bamboo Fungus ZJZS007 cultured on a plate for 19 days in Example 1 provided in this application (magnified 400 times);

[0035] Figure 5 This is the phylogenetic tree of Bamboo Spatholobi ZJZS007 in Example 1 provided in this application. DETAILED DESCRIPTION

[0036] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments, but the present invention is not limited in any way. Any changes or improvements made based on the teachings of the present invention fall within the protection scope of the present invention.

[0037] Example

[0038] Unless otherwise specified, the materials and instruments used in the following examples were obtained from commercial channels; the detection methods used were all existing methods unless otherwise specified.

[0039] Example 1 Isolation, Cultivation, Identification and Preservation of Bambusa ochracea ZJZS007

[0040] Bamboo fungus Creamy-ochreous phallus )ZJZS007 was isolated from a wild Bamboo Fungus leucoderma specimen, which was collected by the applicant in Yibin City, Sichuan Province, China in June 2023. The Bamboo Fungus leucoderma specimen and its isolated strain ZJZS007 were classified and identified through traditional morphological characteristics combined with ITS sequence sequencing.

[0041] 1. Isolation and culture of ZJZS007 strain

[0042] The collected wild Dictyophora alba specimens were used as domesticated materials, and the mycelium of Dictyophora alba ZJZS007 was obtained by tissue separation method. The mycelium was then inoculated on the slant of improved PDA culture medium and cultured at 20-25℃ for 14 days to obtain test tube strains. The test tube strains were inoculated on the slant of improved PDA culture medium and stored at 4-6℃ for future use.

[0043] The formula of improved PDA medium is: glucose 20g / L, peptone 2g / L, yeast extract 2g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 1g / L, agar 20g / L.

[0044] 2. Morphological characteristics

[0045] Morphological characteristics: The fruiting bodies of wild white ochre bamboo fungus specimens are spherical to nearly spherical when young, white to light pink, and turn light purple when injured. They are smooth, like Figure 1As shown. The fruiting body of the white ochre bamboo fungus ZJZS007 cultivated according to the method in Example 2 can reach 24 cm in overall height after maturity. The cap is bell-shaped to umbrella-shaped, with irregular ridges; it is covered with spores, truncated at the top, and has a light yellow disk. The spores are olive brown and mucous. The stipe is nearly columnar, 20~24cm long, 2.0~4.5cm in diameter, and white to light pink. The thallus is oval and smooth, yellow-white to light pink. The skirt hangs down to the thallus, is white to light pink, firm, and reticular, consisting of a polygonal grid with a diameter of 0.7~2.0cm and a thickness of 0.2~0.4cm, as shown Figure 2 shown.

[0046] The white ochre bamboo fungus ZJZS007 was inoculated on a modified PDA medium plate and cultured at 20-25℃ for 19 days until the mycelium covered the plate. Figure 3 As shown, the extracted mycelium was observed under a microscope. Figure 4 As shown, the hyphae are thin-walled, smooth, septate, and have lock-like joints, which meet the hyphae morphology requirements of Bamboo Fungus ZJZS007.

[0047] 3. Molecular Identification

[0048] The invention performs DNA extraction and ITS sequencing on wild white ochre bamboo fungus fruiting body and its mycelium isolate ZJZS007, and the primers used are a primer pair consisting of ITS4 and ITS5, ITS5 (5′-GGAAAAGTCGTAACAAGG -3′) and ITS4 (

[0049] The ITS sequences of the tested samples were submitted to the NCBI database for BLAST sequence comparison analysis. The resulting phylogenetic tree is shown in the figure. Figure 5 As shown, the results showed that the ZJZS007 strain Phallus creamy-ochre GDGM 80700, Creamy-ochreous phallus The homology similarities of L7304 and L7304 were as high as 99.74% and 99.83% respectively. The analysis confirmed that the white ochre bamboo fungus ZJZS007 (CCTCC NO: M20242246) separated in Example 1 was the white ochre bamboo fungus of the present invention. P. creamy-ochreous .

[0050] The ITS sequence of Dictyophora alba strain ZJZS007 (ZJZS007-ITS) is 605 bp long

[0051] aaaaagtcgt aacaaggttt ccgtaggtga acctgcggaa ggatcattgc cgaacgataa 60

[0052] ggaggggaga gggaatctct ctctctcccc gaccgacacc tctgtgcact ctgggagggg 120

[0053] ggacttgtcg aaagaaaaaa ggccccctcc cgttcatacg aacgcctcgc agttctcgcg 180

[0054] cgtgtcagtg aaatcgaaaa tcgtaacaca actttcaaca acggatctct tggctttcgc 240

[0055] atcgatgaag aacgccgcga acgcgcgaaa cgtaatgtga attgcagaat tcagtgaatc 300

[0056] atcgaatctt tgaacgcacc ttgcgctcct cggtattccg aggagcatgc ctgtttgagt 360

[0057] gtcgtgaagt cttatcgaaa aggggggtgt cccctcccct cttttcggac ttggacggtc 420

[0058] ttgccctcgc cctgtcgaag gctcgtcttc aaattcatcg gcgaggttcc ctaaaacccc 480

[0059] ccccattcca gacgtgttaa gtcttgcgtc gcgagggggg aaagaggggg attcccgtcc 540

[0060] gcttcatctc tcatttaaaa acgccttgac ctcaaatcag gcaggactac ccgctgaact 600

[0061] IV. Strain Preservation

[0062] Based on the above morphological characteristics and the classification and identification research of ITS sequences, the strain of the present invention was determined to be Bamboo Spatholobi P. creamy-ochre . Bamboo fungus Creamy-ochreous phallus) ZJZS007, has been deposited in the China Center for Type Culture Collection, the deposit date is October 17, 2024, and the deposit number is CCTCC NO: M20242246; the white ochre bamboo fungus ZJZS007 strain belongs to the Basidiomycetes, Agaricomycetes, Phalloides, Phallaceae, and Phallus.

[0063] Example 2 Cultivation method of fruiting body of Bambusa ochracea ZJZS007

[0064] 1) Mother culture propagation: inoculate the white ochre bamboo fungus ZJZS007 test tube strain on the mother culture medium, and culture it at a temperature range of 24°C in the dark for 15 days to obtain the white ochre bamboo fungus ZJZS007 strain mother culture; wherein the mother culture medium composition is: 20g glucose, 2g peptone, 2g yeast powder, 1g potassium dihydrogen phosphate, 1g magnesium sulfate, 20g agar, and 1L water;

[0065] (2) Propagation of the liquid culture of the white ochre bamboo fungus ZJZS007 strain: The white ochre bamboo fungus ZJZS007 mother culture obtained in step (1) is inoculated into a liquid culture medium, and then cultured at a temperature of 25° C. in the dark for 7 days to obtain a white ochre bamboo fungus ZJZS007 liquid culture; wherein the liquid culture medium is composed of: 20 g glucose, 2 g peptone, 2 g yeast powder, 1 g potassium dihydrogen phosphate, 1 g magnesium sulfate and 1 L water.

[0066] (3) Preparation of cultivation bag of ZJZS007 strain of Bamboo Fungus: 45 mL of the liquid strain of Bamboo Fungus ZJZS007 obtained in step (2) is inoculated into the culture bag culture medium, and cultured at a temperature of 24°C and in the dark to obtain the cultivation bag of Bamboo Fungus ZJZS007; wherein the cultivation material filled in the cultivation bag is composed of: 63 parts by weight of bamboo chips, 26 parts by weight of corn cobs, 10 parts by weight of wheat bran, and 1 part by weight of gypsum. Soak the bamboo chips and corn cobs for 20 hours, and then mix them with wheat bran and gypsum to obtain the cultivation material, the water content of which reaches 65%. Put the culture material into a polypropylene plastic bag (15cm×30cm×0.05cm), the bagging should be appropriately tight, and the bag mouth is sealed with a ring-shaped cotton-free cover;

[0067] (4) Cultivation and harvesting: After the cultivation seeds covered with mycelium are removed from the bags, they are covered with soil for cultivation. After covering with soil, the air humidity is controlled at 50% to 90%, the temperature is 15℃ to 25℃, and the light intensity is 200 to 1000LX. If the temperature is higher than 30℃, ventilation should be carried out in time. After the mycelium in the bag begins to twist and crawl out of the soil surface, drip irrigation or sprinkler irrigation can be used to spray heavy water once, irrigate the covering material thoroughly, and stimulate the formation of primordium. Generally, primordium begins to appear 25 days after sowing, and the fungus can be cultivated. Once the white ochre bamboo fungus breaks the buds and spreads the fungus skirt, it should be harvested in time.

[0068] Example 3 Comparative test on agronomic traits, nutrient content of dried fruiting bodies, and mycelial growth of Dictyophora alba ZJZS007 and existing cultivated Dictyophora alba

[0069] 1. Acquisition of experimental fruiting bodies:

[0070] The existing bamboo fungus varieties used are Qianfeng No. 1 and Zhongjun Bamboo Fungus No. 1. For details of Zhongjun Bamboo Fungus No. 1, see Yue Wansong, Sun Dafeng, Hua Rong, et al. Breeding of a new red-tube bamboo fungus variety 'Zhongjun Bamboo Fungus No. 1' [J]. Journal of Edible Fungi, 2024, 31 (04): 46-54. Qianfeng No. 1 is a commercially available variety purchased on the market.

[0071] Use Qianfeng No. 1, Zhongjun Bamboo Fungus No. 1, and Baihe Bamboo Fungus ZJZS007 to culture until the fruiting body is harvested:

[0072] According to Example 2, the obtained white ochre bamboo fungus ZJZS007 liquid spawn was inoculated on the culture bag culture medium for cultivation and cultivation. The cultivation method was as follows: after the mycelium was full of the culture material, the outer bag of the mushroom sticks was torn open, and the mushroom sticks were placed in a vertical row on the arranged box surface, with 3 rows on each box surface, 12 mushroom bags were planted per square meter, and the soil was covered and cultivated until the mushrooms were produced. The experimental planting area of ​​each sample was 10m 2 .

[0073] 2. Measurement and testing:

[0074] After harvesting, the stipe length, stipe diameter and skirt length of the fruiting bodies of Qianfeng No. 1, Zhongjun Bamboo Sun No. 1 and Baizhe Bamboo Sun ZJZS007 were measured. The measurement results are shown in Table 1. The results obtained are all average values.

[0075] After harvesting the fruiting bodies of Qianfeng No. 1, Zhongjun Bamboo Sun No. 1, and Baizhe Bamboo Sun ZJZS007, the dry products of each sample were taken and the protein, crude polysaccharide, total sugar, selenium, and iron contents in the fruiting bodies were detected according to GB5009.5-2016 National Food Safety Standard for Protein in Food; GB 5009.93-2017 National Food Safety Standard for Selenium in Food; GB 5009.90-2016 National Food Safety Standard for Iron in Food; and GB / T 15672-2009 Total Sugar Content in Edible Fungi. The results are shown in Table 2.

[0076] Table 1

[0077]

[0078] See Table 1. The obtained Zhongjun Bamboo Fungus No. 1, Qianfeng No. 1, and Baihe Bamboo Fungus ZJZS007 are shown in Table 1. The fruiting body of Baihe Bamboo Fungus ZJZS007 cultivated in Example 2 is larger: the average length of the stipe is (22.58±0.83) cm, and the average diameter is (3.25±0.14) cm; the skirt hangs down to the thallus, that is, the length of the skirt is basically the same as the length of the stipe, which is (22.58±0.83) cm. Compared with the demonstration cultivation of other bamboo fungus varieties studied in the prior art, the fruiting body of Baihe Bamboo Fungus ZJZS007 is larger.

[0079] Table 2

[0080]

[0081] As shown in Table 2, the content of crude polysaccharide, total sugar and selenium in the fruiting body of Bamboo Fungus ZJZS007 is higher than that of Qianfeng No. 1 and Zhongjun Bamboo Fungus No. 1, and polysaccharide is the main substance of biological activity of Bamboo Fungus. As one of the essential trace elements in the human body, selenium has a significant impact, and many diseases are closely related to selenium.

[0082] It was shown that Ochre Bamboo Fungus ZJZS007 has high crude polysaccharide, total sugar and selenium contents, which is beneficial to improving the nutritional components of existing bamboo fungus varieties.

[0083] 3. Bacteria bag culture and growth measurement

[0084] The obtained liquid strain of Bamboo Fungus ZJZS007 was inoculated into the culture bag culture medium as described in Example 2 for cultivation according to Example 2; the culture was carried out at a temperature of 25° C. and in the dark to obtain the Bamboo Fungus ZJZS007 culture bag.

[0085] During the fermentation period, observe the growth and color of mycelium, measure the mycelium growth rate by drawing lines, and record the time required to fill the bag. Mycelium growth rate (mm·d -1 ) = mycelium length (mm) / mycelium growth period (d). The growth observation results of Qianfeng No. 1, Zhongjun Bamboo Fungus No. 1, and Baizhe Bamboo Fungus ZJZS007 are shown in Table 3, and the results obtained are all average values.

[0086] Table 3

[0087]

[0088] As shown in Table 3, the mycelium growth rate of ZJZS007 was the highest at 1.86 mm·d-1, and the bag-filling time was the shortest at only 81 days. The mycelium grew well and could effectively resist the growth of foreign bacteria. The mycelium was dense, strong and grew evenly.

[0089] Example 4

[0090] The difference from Example 2 is:

[0091] The mother strain propagation operation includes the following steps: inoculating the test tube strain of Bamboo Fungus dahliae ZJZS007 on the mother strain culture medium, culturing for 18 days at a temperature of 20° C. and in dark conditions to obtain the mother strain of Bamboo Fungus dahliae ZJZS007.

[0092] The mother culture medium is composed of 18g glucose, 2g peptone, 2g yeast powder, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 20g agar, and 1L water.

[0093] The liquid strain propagation operation includes the following steps: the mother strain of the white ochre bamboo fungus ZJZS007 is inoculated into a liquid culture medium, and cultured for 6 days at a temperature of 25° C. in dark conditions to obtain the white ochre bamboo fungus ZJZS007 liquid strain.

[0094] The composition of the liquid culture medium is: 18 g glucose, 2 g peptone, 2 g yeast powder, 1 g potassium dihydrogen phosphate, 1 g magnesium sulfate and 1 L water.

[0095] The inoculation volume of the liquid fungus inoculated into the culture medium is 45 mL / bag.

[0096] The cultivation conditions on the culture medium were 25°C in the dark.

[0097] The cultivation material is filled into the material bag and then inoculated with liquid bacteria; the material bag used is a polypropylene plastic bag.

[0098] Preparation method of the cultivation material: weigh 54 parts by weight of bamboo chips, 30 parts by weight of corn cobs, 15 parts by weight of wheat bran, and 1 part by weight of gypsum;

[0099] After soaking bamboo chips and corn cobs for 24 hours, they are evenly mixed with wheat bran and gypsum to obtain a cultivation material with a water content of 60%.

[0100] After covering the soil, control the cultivation environment: air humidity is 50%~90%, temperature is 18℃~28℃, and light intensity is 200~500LX.

[0101] Example 5

[0102] The difference from Example 2 is:

[0103] The mother strain propagation operation includes the following steps: inoculating the test tube strain of Bamboo Fungus dahliae ZJZS007 on the mother strain culture medium, culturing for 13 days at a temperature of 25° C. and in dark conditions to obtain the mother strain of Bamboo Fungus dahliae ZJZS007.

[0104] The mother culture medium is composed of 20g glucose, 2g peptone, 3g yeast powder, 1g potassium dihydrogen phosphate, 2g magnesium sulfate, 15g agar, and 1L water.

[0105] The liquid strain propagation operation includes the following steps: the mother strain of the white ochre bamboo fungus ZJZS007 is inoculated into a liquid culture medium, and cultured for 10 days at a temperature of 22° C. in dark conditions to obtain the white ochre bamboo fungus ZJZS007 liquid strain.

[0106] The composition of the liquid culture medium is: 20g glucose, 3g peptone, 3g yeast powder, 2g potassium dihydrogen phosphate and 2g magnesium sulfate and 1L water.

[0107] The inoculation amount of liquid bacteria inoculated into the culture medium is 30mL / bag.

[0108] The cultivation conditions on the culture medium were 20°C in the dark.

[0109] The cultivation material is filled into the material bag and then inoculated with liquid bacteria; the material bag used is a polypropylene plastic bag.

[0110] Preparation method of the cultivation material: weigh 60 parts by weight of bamboo chips, 24 parts by weight of corn cobs, 15 parts by weight of wheat bran, and 1 part by weight of gypsum;

[0111] After soaking bamboo chips and corn cobs for 12 hours, they are evenly mixed with wheat bran and gypsum to obtain a cultivation material with a water content of 65%.

[0112] After covering the soil, control the cultivation environment: air humidity is 50%~90%, temperature is 20℃~28℃, and light intensity is 200~2000LX.

[0113] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A new strain of Bamboo Shoots, characterized in that: It is the strain ZJZS007 of Bamboo Spatholobi, classified and named Phallus cremeo-ochraceus ; The deposit number is CCTCC NO: M20242246.

2. A method for acclimating and cultivating a new strain of Bamboo Fungus leucoderma, characterized in that: The following steps are involved: The test tube strain of the white ochre bamboo fungus ZJZS007 as claimed in claim 1 is propagated by mother strain and liquid strain, and the obtained liquid strain is inoculated on the cultivation material for cultivation; Cover the cultivation material covered with mycelium with soil to stimulate the formation of primordium until primordium appears. Continue to cultivate the fungus until the buds of the white ochre bamboo fungus break and the fungus skirt is spread out, then it can be harvested.

3. The method according to claim 2, characterized in that The mother strain propagation operation includes the following steps: inoculating the test tube strain of Bamboo Fungus ZJZS007 on the mother strain culture medium, culturing for 13 to 18 days at a temperature range of 20 to 25° C. and in dark conditions to obtain the mother strain of Bamboo Fungus ZJZS007.

4. The method according to claim 3, characterized in that The mother culture medium consists of 18~20g glucose, 2~3g peptone, 2~3g yeast powder, 1~2g potassium dihydrogen phosphate, 1~2g magnesium sulfate, 15-20g agar, and 1L water.

5. The method according to claim 2, characterized in that: The liquid culture propagation operation includes the following steps: The mother strain of the white ochre bamboo fungus ZJZS007 is inoculated into a liquid culture medium, and cultured at a temperature of 22-25° C. in the dark for 6-10 days to obtain the white ochre bamboo fungus ZJZS007 liquid strain.

6. The method according to claim 5, characterized in that The composition of the liquid culture medium is: 18~20g glucose, 2~3g peptone, 2~3g yeast powder, 1~2g potassium dihydrogen phosphate and 1~2g magnesium sulfate and 1L water.

7. The method according to claim 2, characterized in that The inoculation volume of liquid bacteria in the culture medium is 30~45mL / bag.

8. The method according to claim 2, characterized in that: Preparation method of the cultivation material: weigh 54-63 parts by weight of bamboo chips, 26-30 parts by weight of corn cobs, 10-15 parts by weight of wheat bran, and 1 part by weight of gypsum; Soak bamboo chips and corn cobs for 12 to 24 hours and then mix them with wheat bran and gypsum to obtain a cultivation material with a water content of 60% to 65%.

9. The method according to claim 2, characterized in that: After covering the soil, control the cultivation environment: air humidity between 50% and 90%, temperature between 15℃ and 28℃, light intensity between 200 and 2000LX, and timely ventilation when the temperature is higher than 30℃; After the mycelium in the cultivation material twists and crawls out of the soil surface, use drip irrigation or sprinkler irrigation to spray heavy water at least once to thoroughly water the covering material and stimulate the formation of primordium.

10. The method according to claim 2, characterized in that The cultivation conditions on the culture medium are cultivation at a temperature of 20-25°C and in darkness; The cultivation material is filled into the material bag and then inoculated with liquid bacteria; the material bag used is a polypropylene plastic bag.

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