Stem cell preservation device and preservation method

Abstract

The invention relates to the technical field of biology, in particular to a stem cell preservation device and a preservation method.The stem cell preservation device comprises a preservation barrel and a lifting ring movably arranged on the outer wall of the preservation barrel in a sleeving mode, a first electric push rod is connected between the bottom end of the lifting ring and the upper end of the outer wall of the preservation barrel, and an upper cover is rotationally arranged at the upper end of the lifting ring; a guide-in pipe and a control displayer are arranged on the outer wall of the lower end of the storage barrel. According to the stem cell preservation device and preservation method provided by the invention, when the upper cover is opened and closed, the lifting of the second electric push rod can promote the auxiliary mechanism to generate different effects, when the upper cover is opened and the lifting table moves upwards, liquid nitrogen in the lifting table is basically discharged, and the taking-out of a test tube is not interfered; the lifting tables move downwards, so that part of liquid nitrogen is squeezed into the nitrogen storage block from the lifting tables, the liquid nitrogen can be directly weighed, and the mass of a sample placed every time does not need to be known in advance.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a stem cell preservation device and a preservation method. Background Art

[0002] Stem cells are a type of cell that has unlimited or immortal self-renewal capabilities. In terms of preservation, stem cells need to be stored in liquid nitrogen. Due to the particularity of liquid nitrogen, the temperature in liquid nitrogen is very low. At the same time, stem cells will enter a dormant state in a low-temperature environment and can be preserved indefinitely.

[0003] In the invention with publication number CN115152745A, the purpose of preserving stem cells can be achieved by storing stem cells in a storage barrel and then adding liquid nitrogen into the storage barrel. By starting the electric push rod, the placement rack can be moved upward to prevent the liquid nitrogen from covering the test tubes containing stem cells and affecting the line of sight, so as to avoid taking the wrong test tubes containing stem cells. However, this invention only reduces the surrounding liquid nitrogen by moving the placement rack upward, but when the placement rack of the device moves upward, some liquid nitrogen will still be taken out, and it is necessary to stand for a period of time and wait for the liquid nitrogen to disperse before the corresponding test tube can be accurately taken out. Moreover, each time it is taken out, the liquid nitrogen will be lost, thereby causing loss of liquid nitrogen.

[0004] When in use, it is necessary to regularly detect the liquid nitrogen content in the liquid nitrogen tank, usually using a wooden stick method or a weighing method. In the invention with the publication number CN117141903A, the pop-up wooden stick can be taken to observe the frosting height, and the height of the liquid nitrogen in the storage barrel can be timely known. Compared with the traditional operation of opening the liquid nitrogen barrel cover and inserting the wooden stick to detect the liquid nitrogen content, this method of taking out can effectively reduce the loss of liquid nitrogen caused by opening the cover, thereby avoiding the waste of liquid nitrogen. However, the invention is to make the surface of the wooden stick 5 contact the liquid nitrogen and frost occurs by letting the liquid nitrogen enter the corresponding wooden stick 5 rotating cylinder 3 from the leak hole at the bottom of the leak hole bottom plate 717, and the length of the frost is the height of the liquid nitrogen liquid level, wherein it takes a while for the liquid nitrogen to enter from the leak hole at the bottom of the leak hole bottom plate 717, which reduces the speed of the wooden stick observation method of the detection, and is not as accurate as the weighing method, but the weighing method also takes a lot of time, and the weighing method needs to know the weight of each sample, which reduces the detection speed. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a stem cell preservation device and a preservation method.

[0006] The present invention adopts the following technical scheme, a stem cell storage device, comprising a storage barrel and a lifting ring movably sleeved on the outer wall of the storage barrel, a first electric push rod is connected between the bottom end of the lifting ring and the upper end of the outer wall of the storage barrel, an upper cover is rotatably provided on the upper end of the lifting ring, an introduction tube and a control display are provided on the outer wall of the lower end of the storage barrel, and further comprising:

[0007] A weighing device, wherein the weighing device is arranged at the bottom end of the inner cavity of the storage barrel, and a nitrogen storage block is arranged to slide up and down on the upper end of the storage barrel, and a hose connected to the inner cavity of the nitrogen storage block is connected to the upper end of the nitrogen storage block;

[0008] The auxiliary mechanism is used to discharge part of the liquid nitrogen in the gas storage mechanism into the nitrogen storage block when weighing is required. The auxiliary mechanism includes a fixed block, and the fixed block is fixedly connected to the inner wall of the storage barrel. A fixed connecting plate is fixedly connected between the bottom ends of adjacent fixed blocks, and the outer wall of the hose is fixedly connected to the fixed connecting plate.

[0009] A sealing assembly, when the upper cover is opened, is used to discharge or introduce the liquid nitrogen in the gas storage mechanism through the pressure difference, and when the upper cover is closed, the liquid nitrogen at the upper and lower ends of the test tube can be evenly distributed, and the sealing assembly is arranged on the auxiliary mechanism;

[0010] The gas storage mechanism is used to move the test tube up and down when taking out and storing it. The gas storage mechanism is arranged at the upper end of the inner cavity of the storage barrel. The gas storage mechanism includes a lifting platform, and a second electric push rod is connected between the center of the bottom end of the lifting platform and the storage barrel;

[0011] And a fixing assembly is used for automatically clamping the test tube during storage and gas extraction, and the fixing assembly is arranged in the gas storage mechanism.

[0012] As a further description of the above technical solution: the auxiliary mechanism includes a fixed block, a movable insertion rod is movably inserted into the upper end of the fixed block, and an insertion strip is horizontally slidably arranged in the fixed block at the lower side of the movable insertion rod, a sixth spring is fixedly connected between the end of the insertion strip away from the movable insertion rod and the fixed block, and the end of the insertion strip away from the movable insertion rod is movably inserted into the bottom end of the fixed plate, an extrusion block is slidably arranged in the fixed block on one side of the movable insertion rod, a seventh spring is fixedly connected between the end of the extrusion block close to the movable insertion rod and the fixed block, a sliding sleeve block is slidably arranged on the inclined surface of the bottom end of the extrusion block, a trigger rod is movably inserted in the sliding sleeve block, and an eighth spring is fixedly connected between the upper side wall of the trigger rod and the sliding sleeve block, and the ends of the extrusion block and the sliding sleeve block close to the lifting platform are parallel to the side wall of the fixed block.

[0013] As a further description of the above technical solution: the sealing assembly includes a fixed plate fixedly connected to the fixed block near the side wall of the lifting platform, the lifting platform is movably mounted on the outside of the fixed plate, the outer wall of the fixed plate is in close contact with the inner wall of the lifting platform, an annular airbag is arranged in the fixed plate, and a sub-airbag is arranged on the circumference of the annular airbag, the end of the sub-airbag away from the annular airbag abuts against a top block, a fifth spring is fixed between the end of the top block away from the sub-airbag and the fixed plate, the lower side of the top block abuts against an arc block, and the arc block is slidably arranged in the fixed plate up and down, a fixed insert ring is provided on the lower side of the arc block, and the bottom end of the fixed insert ring is fixed to the bottom of the inner cavity of the lifting platform, an extrusion slope is provided at the bottom end of the arc block, and each of the arc blocks is connected to each other.

[0014] As a further description of the above technical solution: the gas storage mechanism includes a lifting platform, and placement grooves are provided on the upper surface of the lifting platform between adjacent fixed blocks, a sealing column is movably inserted into the side wall of the upper cover close to the lifting platform, and a first spring is fixedly connected between one end of the sealing column located in the upper cover and the upper cover, a lifting column is movably inserted into the bottom end of the lifting platform, and sliding baffles are abutted on two opposite sides of the bottom end of the lifting column, and a fourth spring is fixedly connected between the side wall of the bottom end of the sliding baffle and the lifting platform, an air guide port is provided in the lifting platform on the side of the sliding baffle away from the lifting column, the upper end of the air guide port is communicated with the inner cavity of the lifting platform, and the bottom end of the air guide port is communicated with a hose.

[0015] As a further description of the above technical solution: the fixed component includes a fixed sleeve fixedly connected to the bottom end of the inner cavity of the lifting platform, and sliding blocks are slidably arranged on two opposite sides of the fixed sleeve, and a second spring is fixedly connected between the end of the sliding block close to the air guide port and the fixed sleeve, the upper end of the sliding block is in contact with a lifting trapezoidal rod, and the lifting trapezoidal rod is movably inserted in the fixed sleeve up and down, a fixed plug column is provided on the upper side of the lifting trapezoidal rod, and the upper end of the fixed plug column is fixedly connected to the bottom end of the fixed plate, the upper part of one end of the sliding block away from the lifting trapezoidal rod extends to the upper side of the fixed sleeve, a connecting block is provided when the sliding block slides, and a third spring is fixedly connected between one end of the connecting block and the sliding block, and an arc-shaped clamping block is fixedly connected to the upper end of the connecting block.

[0016] As a further description of the above technical solution: a plurality of the fixing blocks are arranged in a ring shape with equal intervals.

[0017] As a further description of the above technical solution: the weighing device, the second electric push rod and the first electric push rod are all electrically connected to the inside of the control display.

[0018] As a further description of the above technical solution: the sealing column has the same shape and structure as the placement groove.

[0019] In addition, the present invention adopts the following technical solution, a storage method of a stem cell storage device, comprising the following steps:

[0020] S1. When the test tube needs to be taken out or placed, the upper cover is opened, and the lifting platform is moved upward by the second electric push rod, and the stem cell test tube in the lifting platform is also moved upward. When the fixed plate moves downward, the liquid nitrogen in the lifting platform is squeezed into the nitrogen storage block through the air guide port. When the fixed plate moves to the bottom of the inner cavity of the lifting platform, the fixed insert ring is inserted into the fixed plate, and the top end of the fixed insert ring squeezes the arc block upward, and the upper end of the arc block squeezes the top block in the direction away from the airbag, so that the airbag is no longer squeezed, and the gas in the annular airbag enters into the airbag, so that the annular airbag automatically becomes soft, which is convenient for taking out or placing the test tube;

[0021] S2. Then, the stem cell test tubes to be frozen and stored are placed in the placement slots, and then the lifting platform is moved down to the original position, and finally the upper cover is closed again to complete the placement;

[0022] S3. When the liquid nitrogen needs to be weighed, close the upper cover, and move the lifting platform upward for a certain distance through the second electric push rod, so that the lifting platform squeezes the trigger rod upward, and the liquid nitrogen in the lifting platform can be weighed, and the value can be seen on the control display.

[0023] The present invention provides a stem cell preservation device and preservation method through improvement, which has the following improvements and advantages compared with the prior art:

[0024] First, when the upper cover is opened and the lifting platform moves upward, the stem cell test tube in the lifting platform will also move upward. When the lifting platform moves upward, the fixed plate moves downward relative to the lifting platform. The top block is subjected to the elastic force of the fifth spring, which will cause the gas in the scoring airbag to be discharged into the annular airbag, thereby making the outer wall of the annular airbag close to the test tube, so that when the fixed plate moves downward, the liquid nitrogen in the lifting platform is squeezed into the nitrogen storage block through the air guide port. When the test tube is taken out, the liquid nitrogen in the lifting platform is basically discharged, which will not interfere with the removal of the test tube, and will not cause the staff to be frostbitten by the liquid nitrogen. At the same time, each time the test tube is taken out, the liquid nitrogen will basically not be lost, so the freezing cost is further saved, and the need to re-add liquid nitrogen after multiple removals is avoided, which can further improve the efficiency.

[0025] Second, after the upper cover is closed, the upper cover presses the movable rod downward, and the sliding sleeve squeezes one end of the extrusion block into the lifting platform. The volume of the inner cavity of the lifting platform is reduced, so that part of the liquid nitrogen is squeezed from each lifting platform into the nitrogen storage block. The liquid nitrogen can be weighed directly without knowing the mass of the sample placed each time in advance. It is convenient and fast, and the cost is also low.

[0026] To sum up, when the upper cover is opened and closed, the lifting and lowering of the second electric push rod will cause the auxiliary mechanism to produce different effects. When the upper cover is opened, the lifting platform moves upward, the outer wall of the annular airbag is close to the test tube, and the liquid nitrogen in the lifting platform is squeezed into the nitrogen storage block through the air guide port. The liquid nitrogen in the lifting platform is basically discharged, which will not interfere with the removal of the test tube and will not cause frostbite of the staff by the liquid nitrogen. When the upper cover is closed, the lifting platform moves downward, the liquid nitrogen is sucked into the lifting platform, the annular airbag becomes soft, the outer wall of the test tube is wrapped with liquid nitrogen, and the volume of the inner cavity of the lifting platform is reduced, so that part of the liquid nitrogen is squeezed from each lifting platform into the nitrogen storage block, so that the liquid nitrogen can be directly weighed, and there is no need to know the mass of the sample placed each time in advance, which is convenient and quick. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The present invention will be further explained below in conjunction with the accompanying drawings and embodiments:

[0028] Figure 1 A schematic diagram of a stem cell storage device and storage method provided in an embodiment of the present invention Figure 1 ;

[0029] Figure 2 A schematic diagram of a stem cell storage device and storage method provided in an embodiment of the present invention Figure 2 ;

[0030] Figure 3 A schematic diagram of the structure of a sealing column provided in an embodiment of the present invention;

[0031] Figure 4 A schematic diagram of the structure of a nitrogen storage block provided in an embodiment of the present invention;

[0032] Figure 5 A schematic diagram of the structure of a second electric push rod provided in an embodiment of the present invention;

[0033] Figure 6 A schematic diagram of the structure of a fixing plate provided in an embodiment of the present invention;

[0034] Figure 7 A three-dimensional cross-sectional view of a fixed block and a lifting platform provided in an embodiment of the present invention;

[0035] Figure 8 A schematic diagram of the structure of the air guide port provided in an embodiment of the present invention;

[0036] Fig. 9 A schematic diagram of the structure of the airbag provided by an embodiment of the present invention;

[0037] Fig.10 for Figure 7 The enlarged view of point A in the middle;

[0038] Fig.11 for Figure 8Enlarged view of point B in the middle.

[0039] In the figure: 1. Preservation barrel; 2. Lifting ring; 3. First electric push rod; 4. Upper cover; 5. Gas storage mechanism; 51. Lifting platform; 52. Placement slot; 53. Sealing column; 54. First spring; 55. Fixing assembly; 551. Fixing sleeve; 552. Sliding block; 553. Second spring; 554. Lifting ladder rod; 555. Fixing column; 556. Arc clamping block; 557. Connecting block; 558. Third spring; 56. Lifting column; 57. Sliding stop rod; 58. Fourth spring; 6. Control display; 7. Introductory tube; 8. Auxiliary mechanism; 8 1. Fixed block; 82. Movable plug rod; 83. Sealing assembly; 831. Fixed plate; 832. Annular airbag; 833. Sub-airbag; 834. Fifth spring; 835. Arc block; 836. Fixed plug ring; 837. Extrusion ramp; 838. Top block; 84. Sixth spring; 85. Extrusion block; 86. Seventh spring; 87. Sliding sleeve block; 88. Trigger rod; 89. Eighth spring; 810. Insert strip; 9. Nitrogen storage block; 10. Weighing device; 11. Hose; 12. Fixed connecting plate; 13. Second electric push rod; 14. Air guide port. DETAILED DESCRIPTION

[0040] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the present invention is further described below with reference to specific diagrams. It should be noted that the embodiments and features in the embodiments of the present application can be combined with each other without conflict.

[0041] See also Figure 1 - Fig.11 The embodiment of the present invention provides a technical solution: a stem cell preservation device and preservation method, comprising a preservation barrel 1 and a lifting ring 2 movably sleeved on the outer wall of the preservation barrel 1, a first electric push rod 3 is connected between the bottom end of the lifting ring 2 and the upper end of the outer wall of the preservation barrel 1, an upper cover 4 is rotatably provided on the upper end of the lifting ring 2, an introduction tube 7 and a control display 6 are provided on the outer wall of the lower end of the preservation barrel 1, and further comprising:

[0042] The weighing device 10 is arranged at the bottom end of the inner cavity of the preservation barrel 1. The weighing device 10, the second electric push rod 13 and the first electric push rod 3 are all electrically connected to the inside of the control display 6. The upper end of the preservation barrel 1 is abutted against a nitrogen storage block 9, and the nitrogen storage block 9 is slidably arranged up and down. The upper end of the nitrogen storage block 9 is connected to a hose 11 connected to the inner cavity of the nitrogen storage block 9. The auxiliary mechanism 8 is used to discharge part of the liquid nitrogen in the gas storage mechanism 5 into the nitrogen storage block 9 when weighing is required. The auxiliary mechanism 8 includes a fixed block 81, and the fixed block 81 is fixedly connected to the inner wall of the preservation barrel 1. A fixed connecting plate 12 is fixedly connected between the bottom ends of adjacent fixed blocks 81. The outer wall of the hose 11 is fixedly connected to the fixed In the connecting plate 12, there is a sealing component 83, which is used to discharge or introduce the liquid nitrogen in the gas storage mechanism 5 through the pressure difference when the upper cover 4 is opened. When the upper cover 4 is closed, the liquid nitrogen at the upper and lower ends of the test tube can be evenly distributed. The sealing component 83 is arranged on the auxiliary mechanism 8, and the gas storage mechanism 5 is used to move the test tube up and down when taking out and storing. The gas storage mechanism 5 is arranged at the upper end of the inner cavity of the preservation barrel 1. The gas storage mechanism 5 includes a lifting platform 51, and a second electric push rod 13 is connected between the center of the bottom end of the lifting platform 51 and the preservation barrel 1, as well as a fixing component 55, which is used for automatically clamping the test tube during storage and vacuuming. The fixing component 55 is arranged in the gas storage mechanism 5.

[0043] Specifically, when the upper cover 4 is opened and closed, the lifting of the second electric push rod 13 causes the auxiliary mechanism 8 to produce different effects. When the upper cover 4 is opened, after the lifting platform 51 moves upward, the outer wall of the annular airbag 832 is in close contact with the test tube, and the liquid nitrogen in the lifting platform 51 is squeezed into the nitrogen storage block 9 through the air guide port 14. The liquid nitrogen in the lifting platform 51 is basically discharged, which will not interfere with the removal of the test tube and will not cause the staff to be frostbitten by the liquid nitrogen. When the fixing plate 831 moves to the bottom of the inner cavity of the lifting platform 51, the annular airbag 832 automatically becomes soft, which is convenient for taking out the test tube from the lifting platform 51 and placing a new test tube;

[0044] When the upper cover 4 is closed, the lifting platform 51 moves downward, the liquid nitrogen is sucked into the lifting platform 51, the annular airbag 832 becomes soft, the outer wall of the test tube is coated with liquid nitrogen, and the volume of the inner cavity of the lifting platform 51 is reduced, so that part of the liquid nitrogen is squeezed from each lifting platform 51 into the nitrogen storage block 9, and the liquid nitrogen can be directly weighed.

[0045] In another embodiment provided by the present invention, the auxiliary mechanism 8 includes a fixed block 81, and the fixed block 81 is arranged in a ring shape with a plurality of equal intervals. A movable plug rod 82 is movably inserted in the upper end of the fixed block 81, and a plug strip 810 is arranged on the lower side of the movable plug rod 82 in the fixed block 81 for horizontal sliding. A sixth spring 84 is fixedly connected between the end of the plug strip 810 away from the movable plug rod 82 and the fixed block 81, and the end of the plug strip 810 away from the movable plug rod 82 is movably inserted in the bottom end of the fixed plate 831, and the movable plug rod 82 is movably inserted in the lower end of the fixed plate 831. On one side of the movable insertion rod 82, an extrusion block 85 is slidably arranged in the fixed block 81, and a seventh spring 86 is fixedly connected between the end of the extrusion block 85 close to the movable insertion rod 82 and the fixed block 81, and a sliding sleeve block 87 is slidably arranged on the inclined surface at the bottom end of the extrusion block 85. A trigger rod 88 is movably inserted in the sliding sleeve block 87, and an eighth spring 89 is fixedly connected between the side wall of the upper end of the trigger rod 88 and the sliding sleeve block 87. The extrusion block 85 and the end of the sliding sleeve block 87 close to the lifting platform 51 are parallel to the side wall of the fixed block 81.

[0046] The sealing assembly 83 includes a fixing plate 831 fixedly connected to the fixing block 81 on the side wall near the lifting platform 51, and the lifting platform 51 is movably sleeved on the outer side of the fixing plate 831, and the outer wall of the fixing plate 831 is in close contact with the inner wall of the lifting platform 51, an annular airbag 832 is arranged in the fixing plate 831, and a sub-airbag 833 is arranged on the circumference of the annular airbag 832, and the sub-airbag 833 is arranged on the side connected to the annular airbag 832, and the end of the sub-airbag 833 away from the annular airbag 832 abuts against a top block 838, and a fifth spring 834 is fixedly connected between the end of the top block 838 away from the sub-airbag 833 and the fixing plate 831, and an arc block 835 abuts against the lower side of the top block 838, and the arc block 835 is slidably arranged in the fixing plate 831 up and down, a fixing insert ring 836 is arranged on the lower side of the arc block 835, and the bottom end of the fixing insert ring 836 is fixedly connected to the bottom of the inner cavity of the lifting platform 51, and an extrusion slope 837 is arranged at the bottom of the arc block 835, and each arc block 835 is connected to each other.

[0047] Specifically, after the upper cover 4 is closed, the upper cover 4 presses the movable plug rod 82 downward, and the sliding sleeve block 87 squeezes one end of the extrusion block 85 into the lifting platform 51. The volume of the inner cavity of the lifting platform 51 is reduced, so that part of the liquid nitrogen is squeezed from each lifting platform 51 into the nitrogen storage block 9. The liquid nitrogen can be weighed directly without knowing the mass of the sample placed each time in advance. It is convenient and fast, and the cost is also low.

[0048] In another embodiment provided by the present invention, the gas storage mechanism 5 includes a lifting platform 51, and placement grooves 52 are provided between adjacent fixed blocks 81 on the upper surface of the lifting platform 51, and a sealing column 53 is movably inserted into the side wall of the upper cover 4 close to the lifting platform 51. The sealing column 53 has the same shape and structure as the placement groove 52, and one end of the sealing column 53 located in the upper cover 4 is fixedly connected to the upper cover 4 with a first spring 54, and a lifting column 56 is movably inserted into the bottom end of the lifting platform 51, and the two opposite sides of the bottom end of the lifting column 56 are both in contact with sliding baffles 57, and a fourth spring 58 is fixedly connected between the side wall of the bottom end of the sliding baffle 57 and the lifting platform 51, and an air guide port 14 is provided in the lifting platform 51 on the side of the sliding baffle 57 away from the lifting column 56, the upper end of the air guide port 14 is connected to the inner cavity of the lifting platform 51, and the bottom end of the air guide port 14 is connected to the hose 11.

[0049] The fixing assembly 55 includes a fixing sleeve 551 fixed to the bottom end of the inner cavity of the lifting platform 51, and sliding blocks 552 are slidably arranged on two opposite sides of the fixing sleeve 551. A second spring 553 is fixedly connected between the end of the sliding block 552 close to the air guide port 14 and the fixing sleeve 551. The upper end of the sliding block 552 abuts against a lifting trapezoidal rod 554, and the lifting trapezoidal rod 554 is movably inserted in the fixing sleeve 551 up and down. A fixing plug column 555 is provided on the upper side of the lifting trapezoidal rod 554, and the upper end of the fixing plug column 555 is fixed to the bottom end of the fixing plate 831. The upper part of one end of the sliding block 552 away from the lifting trapezoidal rod 554 extends to the upper side of the fixing sleeve 551. When the sliding block 552 slides, a connecting block 557 is provided, and a third spring 558 is fixedly connected between one end of the connecting block 557 and the sliding block 552, and an arc-shaped clamping block 556 is fixedly connected to the upper end of the connecting block 557.

[0050] Specifically, when no test tube is placed in the lifting platform 51 , the sliding blocking rod 57 will be close to the air guide port 14 due to the elastic force of the fourth spring 58 , so that the air guide port 14 is blocked, thereby preventing liquid nitrogen from being wasted when there is no test tube in the lifting platform 51 .

[0051] Working principle: When using the device, first move the lifting ring 2 and the upper cover 4 upwards through the first electric push rod 3, then rotate the upper cover 4 to open it, so that the sealing column 53 is pulled out from the placement groove 52, and then move the lifting platform 51 upwards through the second electric push rod 13, and then place the stem cell test tube that needs to be frozen and stored in the placement groove 52, and then move the lifting platform 51 downward to the original position, and finally cover the upper cover 4 again to complete the placement. When it is necessary to weigh the liquid nitrogen, move the lifting platform 51 upwards for a distance through the second electric push rod 13, so that the lifting platform 51 squeezes the trigger rod 88 upwards, and the liquid nitrogen in the lifting platform 51 can be weighed, and the value can be seen on the control display 6;

[0052] When the upper cover 4 is opened and the lifting platform 51 moves upward, the stem cell test tube in the lifting platform 51 will also move upward. When the lifting platform 51 moves upward, the fixed plate 831 moves downward relative to the lifting platform 51, and the top block 838 is subjected to the elastic force of the fifth spring 834, which will cause the gas in the scoring airbag 833 to be discharged into the annular airbag 832, thereby making the outer wall of the annular airbag 832 close to the test tube, so that when the fixed plate 831 moves downward, the liquid nitrogen in the lifting platform 51 is squeezed into the nitrogen storage block 9 through the air guide port 14, and finally the bottom end of the fixed plate 831 blocks the upper end of the air guide port 14, and when the fixed plate 831 moves to the bottom of the inner cavity of the lifting platform 51, the fixed insert ring 836 is inserted into the fixed plate 831, and the top end of the fixed insert ring 836 pushes the arc block 835 upward Extrusion: the upper end of the arc block 835 squeezes the top block 838 in the direction away from the sub-air bag 833, so that the sub-air bag 833 is no longer squeezed, and the gas in the annular air bag 832 enters the sub-air bag 833, so that the annular air bag 832 automatically becomes soft, which is convenient for taking out the test tube from the lifting platform 51 and placing a new test tube. When the test tube is taken out, the liquid nitrogen in the lifting platform 51 is basically discharged, which will not interfere with the taking out of the test tube, and will not cause the staff to be frostbitten by the liquid nitrogen. At the same time, each time it is taken out, the liquid nitrogen will basically not be lost, so the freezing cost is further saved, and the need to re-add liquid nitrogen after multiple taking out is avoided, which can further improve the efficiency. In addition, the liquid nitrogen will not be mixed with air by discharging and introducing liquid nitrogen in this way;

[0053] When the fixed plate 831 is located at the bottom of the lifting platform 51, the fixed plug 555 will squeeze the sliding block 552 toward the direction close to the second spring 553 through the lifting trapezoidal rod 554, so as to facilitate the placement of the test tube. Afterwards, when the fixed plate 831 leaves the bottom of the lifting platform 51, the sliding blocks 552 on both sides will move toward the test tube, so that the arc-shaped clamping block 556 can clamp the test tube, and the test tube will not be driven upward by the annular airbag 832. The third spring 558 is provided to clamp test tubes of various diameters.

[0054] Before the upper cover 4 is closed, the lifting platform 51 is moved downward so that the fixing plate 831 is located at the outer wall of the top of the test tube, and the liquid nitrogen is sucked into the lifting platform 51. After the upper cover 4 is closed, the bottom end of the sealing column 53 will be against the upper end of the test tube, and the upper cover 4 will press the movable plug rod 82 downward, so that the movable plug rod 82 squeezes the inserting strip 810 and the trigger rod 88 in the direction away from the movable plug rod 82, and the inserting strip 810 squeezes the arc block 835 upward, so that the top block 838 is away from the airbag 833, so that the annular airbag 832 becomes soft again. When weighing is required, the second electric push rod is used to 13 Move the lifting platform 51 upward for a distance, and the annular airbag 832 becomes soft so that even if the annular airbag 832 moves, the outer wall of the test tube can be covered with liquid nitrogen. The lifting platform 51 squeezes the trigger rod 88 upward, and the trigger rod 88 drives the sliding sleeve block 87 upward. The sliding sleeve block 87 squeezes one end of the squeezing block 85 into the lifting platform 51, and the volume of the inner cavity of the lifting platform 51 is reduced, so that part of the liquid nitrogen is squeezed from each lifting platform 51 into the nitrogen storage block 9, so that the liquid nitrogen can be directly weighed, and there is no need to know the mass of the sample placed each time in advance, which is convenient and quick.

[0055] A storage method of a stem cell storage device comprises the following steps:

[0056] S1. When it is necessary to take out or place the test tube, the upper cover 4 is opened, and after the lifting platform 51 is moved upward by the second electric push rod 13, the stem cell test tube in the lifting platform 51 will also move upward. When the fixed plate 831 moves downward, the liquid nitrogen in the lifting platform 51 is squeezed into the nitrogen storage block 9 through the air guide port 14. When the fixed plate 831 moves to the bottom of the inner cavity of the lifting platform 51, the fixed insert ring 836 is inserted into the fixed plate 831, and the top end of the fixed insert ring 836 squeezes the arc block 835 upward, and the upper end of the arc block 835 squeezes the top block 838 in the direction away from the airbag 833, so that the airbag 833 is no longer squeezed, and the gas in the annular airbag 832 enters the airbag 833, so that the annular airbag 832 automatically becomes soft, which is convenient for taking out or placing the test tube;

[0057] S2, then place the stem cell test tubes to be frozen and stored in the placement slot 52, then move the lifting platform 51 downward to the original position, and finally cover the upper cover 4 again to complete the placement;

[0058] S3. When the liquid nitrogen needs to be weighed, close the upper cover 4, and move the lifting platform 51 upward for a distance through the second electric push rod 13, so that the lifting platform 51 presses the trigger rod 88 upward, and the liquid nitrogen in the lifting platform 51 can be weighed, and the value can be seen on the control display 6.

[0059] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and the description in the specification are only to illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A stem cell storage device, comprising a storage barrel (1) and a lifting ring (2) movably mounted on the outer wall of the storage barrel (1), a first electric push rod (3) is connected between the bottom end of the lifting ring (2) and the upper end of the outer wall of the storage barrel (1), an upper cover (4) is rotatably arranged on the upper end of the lifting ring (2), an introduction tube (7) and a control display (6) are arranged on the outer wall of the lower end of the storage barrel (1), characterized in that: Also includes: A weighing device (10), the weighing device (10) being arranged at the bottom end of the inner cavity of the storage barrel (1), the upper end of the storage barrel (1) being provided with a nitrogen storage block (9) in contact therewith, and the nitrogen storage block (9) being arranged to slide up and down, and the upper end of the nitrogen storage block (9) being connected to a hose (11) communicating with the inner cavity of the nitrogen storage block (9); The auxiliary mechanism (8) is used to discharge part of the liquid nitrogen in the gas storage mechanism (5) into the nitrogen storage block (9) when weighing is required, the auxiliary mechanism (8) comprising a fixed block (81), and the fixed block (81) is fixedly connected to the inner wall of the storage barrel (1), a fixed connecting plate (12) is fixedly connected between the bottom ends of adjacent fixed blocks (81), and the outer wall of the hose (11) is fixedly connected to the inside of the fixed connecting plate (12); A sealing assembly (83) is used to discharge or introduce liquid nitrogen in the gas storage mechanism (5) by pressure difference when the upper cover (4) is opened, and to evenly distribute the liquid nitrogen at the upper and lower ends of the test tube when the upper cover (4) is closed. The sealing assembly (83) is arranged on the auxiliary mechanism (8); An air storage mechanism (5) is used for moving the test tube up and down when taking out and storing the test tube. The air storage mechanism (5) is arranged at the upper end of the inner cavity of the storage barrel (1). The air storage mechanism (5) comprises a lifting platform (51), and a second electric push rod (13) is connected between the center of the bottom end of the lifting platform (51) and the storage barrel (1); And a fixing assembly (55) for automatically clamping the test tube during storage and gas extraction, wherein the fixing assembly (55) is arranged in the gas storage mechanism (5).

2. A stem cell storage device according to claim 1, characterized in that: The auxiliary mechanism (8) comprises a fixed block (81), a movable insertion rod (82) is movably inserted at the upper end of the fixed block (81), a plug strip (810) is horizontally slidably arranged at the lower side of the movable insertion rod (82) in the fixed block (81), a sixth spring (84) is fixedly connected between one end of the plug strip (810) away from the movable insertion rod (82) and the fixed block (81), one end of the plug strip (810) away from the movable insertion rod (82) is movably inserted at the bottom end of the fixed plate (831), and one side of the movable insertion rod (82) is located in the fixed block (81). An extrusion block (85) is slidably arranged, and a seventh spring (86) is fixedly connected between one end of the extrusion block (85) close to the movable insertion rod (82) and the fixed block (81); a sliding sleeve block (87) is slidably arranged on the inclined surface at the bottom end of the extrusion block (85); a trigger rod (88) is movably inserted in the sliding sleeve block (87); an eighth spring (89) is fixedly connected between the side wall of the upper end of the trigger rod (88) and the sliding sleeve block (87); and one end of the extrusion block (85) and the sliding sleeve block (87) close to the lifting platform (51) are both parallel to the side wall of the fixed block (81).

3. A stem cell storage device according to claim 1, characterized in that: The sealing assembly (83) comprises a fixing plate (831) fixedly connected to the fixing block (81) on the side wall close to the lifting platform (51); the lifting platform (51) is movably sleeved on the outside of the fixing plate (831) up and down; the outer wall of the fixing plate (831) is in close contact with the inner wall of the lifting platform (51); an annular airbag (832) is arranged in the fixing plate (831); and a sub-airbag (833) is arranged on the circumference of the annular airbag (832); one end of the sub-airbag (833) away from the annular airbag (832) abuts against a top block (838); the top block (838) is provided to seal the fixing plate (831). A fifth spring (834) is fixedly connected between one end of the block (838) away from the airbag (833) and the fixed plate (831); an arc block (835) is abutted against the lower side of the top block (838), and the arc block (835) is slidably arranged in the fixed plate (831) up and down; a fixed insert ring (836) is provided on the lower side of the arc block (835), and the bottom end of the fixed insert ring (836) is fixedly connected to the bottom of the inner cavity of the lifting platform (51); an extrusion slope (837) is provided at the bottom end of the arc block (835), and each of the arc blocks (835) is connected to each other.

4. A stem cell storage device according to claim 1, characterized in that: The gas storage mechanism (5) comprises a lifting platform (51), the upper surface of the lifting platform (51) is provided with placement grooves (52) between adjacent fixed blocks (81), a sealing column (53) is movably inserted into the side wall of the upper cover (4) close to the lifting platform (51), and a first spring (54) is fixedly connected between one end of the sealing column (53) located inside the upper cover (4) and the upper cover (4), and a lifting column (56) is movably inserted into the bottom end of the lifting platform (51). The two opposite sides of the bottom end of the lifting column (56) are both in contact with sliding baffles (57), and a fourth spring (58) is fixedly connected between the side wall of the bottom end of the sliding baffle (57) and the lifting platform (51). An air guide port (14) is provided in the lifting platform (51) on the side of the sliding baffle (57) away from the lifting column (56), and the upper end of the air guide port (14) is connected to the inner cavity of the lifting platform (51), and the lower end of the air guide port (14) is connected to the hose (11).

5. A stem cell storage device according to claim 1, characterized in that: The fixing assembly (55) comprises a fixing sleeve (551) fixedly connected to the bottom end of the inner cavity of the lifting platform (51), and sliding blocks (552) are slidably arranged on two opposite sides of the fixing sleeve (551), and a second spring (553) is fixedly connected between the end of the sliding block (552) close to the air guide port (14) and the fixing sleeve (551), and the upper end of the sliding block (552) is in contact with a lifting trapezoidal rod (554), and the lifting trapezoidal rod (554) is inserted into the fixing sleeve (551) in a manner that the lifting platform (51) can be moved up and down. A fixed plug post (555) is provided on the upper side of the trapezoidal rod (554), and the upper end of the fixed plug post (555) is fixedly connected to the bottom end of the fixed plate (831). The upper part of one end of the sliding block (552) away from the lifting trapezoidal rod (554) extends to the upper side of the fixed sleeve (551). When the sliding block (552) slides inside, a connecting block (557) is provided, and a third spring (558) is fixedly connected between one end of the connecting block (557) and the sliding block (552), and an arc-shaped clamping block (556) is fixedly connected to the upper end of the connecting block (557).

6. A stem cell storage device according to claim 2, characterized in that: A plurality of the fixing blocks (81) are arranged in a ring shape at equal intervals.

7. A stem cell storage device according to claim 1, characterized in that: The weighing device (10), the second electric push rod (13) and the first electric push rod (3) are all electrically connected to the inside of the control display (6).

8. A stem cell storage device according to claim 4, characterized in that: The sealing column (53) has the same shape and structure as the placement groove (52).

9. A method for preserving a stem cell storage device, based on a power cord production conveying device according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. When it is necessary to take out or place the test tube, the upper cover (4) is opened, and the lifting platform (51) is moved upward by the second electric push rod (13), and the stem cell test tube in the lifting platform (51) is also moved upward. When the fixed plate (831) moves downward, the liquid nitrogen in the lifting platform (51) is squeezed into the nitrogen storage block (9) through the air guide port (14). When the fixed plate (831) moves to the bottom of the inner cavity of the lifting platform (51), the fixed insert ring (836) is ) is inserted into the fixing plate (831), the top end of the fixing insert ring (836) presses the arc block (835) upwards, and the upper end of the arc block (835) presses the top block (838) in a direction away from the airbag (833), so that the airbag (833) is no longer squeezed, and the gas in the annular airbag (832) enters the airbag (833), so that the annular airbag (832) automatically becomes soft, which facilitates the removal or placement of the test tube; S2, then place the stem cell test tubes to be frozen and stored in the placement slot (52), then move the lifting platform (51) downward to the original position, and finally cover the upper cover (4) again to complete the placement; S3. When the liquid nitrogen needs to be weighed, the upper cover (4) is closed, and the lifting platform (51) is moved upward for a certain distance by the second electric push rod (13), so that the lifting platform (51) presses the trigger rod (88) upward, and the liquid nitrogen in the lifting platform (51) can be weighed, and the value can be seen on the control display (6).

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