Picea flower monochamus alternatus attractant as well as preparation method and application thereof
The spruce flower tundra lure attractant composed of (1R)-(+)-α-pinene, 3-methylpentan, 2-undecoxy-1-ethanol and paraffin oil was solved. In the technology, there are problems such as complex production process, high cost and unstable trapping effect, which has achieved effective control of spruce flower ink pine nuts and curb the spread of pine nematodes.
Patent Information
- Application Number
- CN202411600950.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-11
- Publication Date
- 2025-02-18
AI Technical Summary
In the prior art, the production process of spruce flower-formed celestial ox attractants is complex, has high cost, and has unstable trapping effect. The impact on different species of spruce flower-formed ox is quite different, making it difficult to effectively control the forest of spruce flower-formed ox. Insect mouth density.
Using spruce flower scented bullock attractant composed of (1R)-(+)-α-pinene, 3-methylpentan, 2-undecoxy-1-ethanol and paraffin oil, through simple Prepared by mixing and stirring, loaded into a polyethylene sustained-release bag, and used to lure and kill spruce flower ink oxen.
It has achieved a spruce flower worm attractant with simple production technology, low cost, high trapping effect, safe and environmentally friendly, effectively controlling the forest insect density of spruce flower worms and curbing the spread and spread of pine nematode disease.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of pest control, and particularly relates to a spruce-flowered Monochamus alternatus attractant, a preparation method and an application thereof. Background Art
[0002] Pine wood nematodes are pathogens that cause pine wood nematode disease, a devastating disease of pine trees. Their targets include pine species such as Masson pine, black pine, Korean pine, Chinese pine, Scots pine, and Chinese pine, which seriously threaten the safety of pine forest resources, causing the destruction of pine forest resources and huge economic losses. The occurrence and spread of pine wood nematode disease are closely related to host plants and vector insects. At present, controlling vector insects to prevent the spread of pine wood nematode disease is still an effective way. There are many insects that can carry pine wood nematodes in nature, but 13 species have been reported as vector insects, all of which are species of the genus Monochamus, mainly pine Monochamus and spruce Monochamus. At the same time, spruce Monochamus is also a serious borer pest in forestry and is widely distributed. Spruce Monochamus alone can also cause pine wilt and death. Therefore, the development and use of attractants to kill spruce Monochamus and the pathogenic nematodes it carries is an effective prevention and control measure that can protect pine wood.
[0003] At present, the reported ingredients such as α-pinene, β-pinene, 3-carene, β-phellandrene, 2-undecyloxy-1-ethanol, terpinene, myrcene, ethanol, phenylethanol, terpineol, etc. are all considered to have an attracting effect on longhorn beetle insects. However, combining different ingredients or mixing them in different ratios will result in a large difference in the trapping effect, and even in the case of no effect or a repellent effect. In addition, combining different ingredients or mixing them in different ratios will also have a large difference in the impact on specific longhorn beetle species. Therefore, it is urgent to provide a spruce-flowered Monochamus alternatus attractant with a simple production process, low cost, high trapping effect, safety, and environmental protection, which has significant market significance for controlling the insect population density of spruce-flowered Monochamus alternatus forests and protecting the planting benefits of pine wood. Summary of the invention
[0004] In order to solve the problems existing in the prior art, the purpose of the present invention is to provide a spruce-flowered Monochamus alternatus attractant, which has a simple production process, low cost, high trapping effect, safety and environmental protection.
[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0006] The invention provides a spruce flower monochamus attractant, which is prepared from the following raw materials by volume: 1-2 parts of (1R)-(+)-alpha-pinene, 4-6 parts of 3-methylpentane, 2-4 parts of 2-undecanyloxy-1-ethanol and 88-93 parts of paraffin oil.
[0007] The present invention also provides a method for preparing the above-mentioned Monochamus alternatus attractant, which comprises the following steps: mixing the raw materials in proportion, stirring evenly, and packing the mixture into a polyethylene sustained-release bag.
[0008] Preferably, the stirring speed is 80 to 120 rpm, and the stirring time is 2 to 8 min.
[0009] Preferably, the polyethylene sustained-release bag is a dark brown polyethylene sustained-release bag.
[0010] The present invention also provides the use of the above-mentioned spruce-flowered Monochamus alternatus attractant or the above-mentioned preparation method in preparing a product for luring and killing spruce-flowered Monochamus alternatus.
[0011] The present invention also provides a product for luring and killing spruce-flowered Monochamus alternatus, comprising a spruce-flowered Monochamus alternatus attractant and a beetle trapping device; the spruce-flowered Monochamus alternatus attractant is the above-mentioned spruce-flowered Monochamus alternatus attractant or the spruce-flowered Monochamus alternatus attractant prepared by the above-mentioned preparation method.
[0012] The invention also provides a method for luring and killing spruce-flowered Monochamus alternatus in the forest, and the product is placed in the forest.
[0013] Preferably, the dosage of the product is 8-15 mL / group, 1-2 groups / hm 2 .
[0014] Preferably, the forest includes Korean pine forest, Chinese pine forest, Larix gmelinii forest and Larix olgensis forest.
[0015] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:
[0016] The invention is based on four compounds, namely, (1R)-(+)-α-pinene, 3-methylpentane, 2-undecyloxy-1-ethanol and paraffin oil, and provides a spruce-flowered Monochamus alternatus attractant with simple production process, low cost, high trapping effect, safety and environmental protection. Through indoor Y-tube competitive test experiments and forest experiments, it is found that the spruce-flowered Monochamus alternatus attractant provided by the invention can effectively control the insect population density of spruce-flowered Monochamus alternatus in the forest, curb the spread and transmission of pine wood nematode disease, and has significant market significance for controlling the insect population density of spruce-flowered Monochamus alternatus in the forest and protecting the planting benefits of pine wood. DETAILED DESCRIPTION
[0017] The present invention provides a spruce-flowered Monochamus alternatus attractant, which is prepared from the following raw materials by volume: 1 to 2 parts of (1R)-(+)-α-pinene, 4 to 6 parts of 3-methylpentane, 2 to 4 parts of 2-undecyloxy-1-ethanol, and 88 to 93 parts of paraffin oil. The raw materials of the spruce-flowered Monochamus alternatus attractant are preferably: 1.2 to 1.5 parts of (1R)-(+)-α-pinene, 5 to 5.5 parts of 3-methylpentane, 2.2 to 3.5 parts of 2-undecyloxy-1-ethanol, and 90 to 92 parts of paraffin oil by volume.
[0018] The present invention also provides a method for preparing the above-mentioned spruce-flowered Monochamus alternatus attractant, comprising the following steps: the raw materials are mixed in proportion, stirred evenly, and loaded into a polyethylene sustained-release bag. The stirring speed of the present invention is 80 to 120 rpm, preferably 90 to 110 rpm; the stirring time is 2 to 8 min, preferably 5 min. The stirring and mixing of the present invention is preferably carried out at room temperature. The polyethylene sustained-release bag of the present invention is preferably a dark brown polyethylene sustained-release bag to prevent the attractant compound from being affected by light.
[0019] The present invention also provides the use of the above-mentioned spruce-flowered Monochamus alternatus attractant or the above-mentioned preparation method in preparing a product for luring and killing spruce-flowered Monochamus alternatus.
[0020] The present invention also provides a product for luring and killing spruce beetle, including a spruce beetle attractant and a beetle trapping device; the spruce beetle attractant is the spruce beetle attractant or the spruce beetle attractant prepared by the above preparation method. The beetle trapping device of the present invention can be any device that can hold the beetle attractant, and can also be a conventional commercially available beetle trap.
[0021] The present invention also provides a method for luring and killing spruce-flowered Monochamus alternatus in the forest: the above product is placed in the forest. That is, the longhorn beetle attractant is placed in a longhorn beetle trapping device, and the longhorn beetle trapping device is placed in the forest. The dosage of the product of the present invention is 8-15 mL / group, 1-2 groups / hm 2 , preferably 10mL / group, 1.3-1.5 groups / hm 2 That is, based on the effective trapping radius of 50m, the effective trapping area of each group (10mL) of trapping core is 7850m 2 The product of the present invention is suitable for Korean pine forest, Chinese pine forest, Scots pine forest and larch forest.
[0022] The technical solutions in the present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0023] In the specific embodiment of the present invention, (1R)-(+)-α-pinene was purchased from Beijing Bailingwei Technology Co., Ltd. with a product batch number of LEC0V33; 3-methylpentane was purchased from Beijing Bailingwei Technology Co., Ltd. with a product batch number of OTEPJ-AM; 2-undecyloxy-1-ethanol was purchased from Beijing Bailingwei Technology Co., Ltd. with a product batch number of LU50W27; paraffin oil was purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. with a product batch number of BCCG8556; dark brown polyethylene sustained-release bags were purchased from Zhongjie Sifang Biotechnology Co., Ltd., with a specification size of 10.0cm×5.5cm; longhorn beetle trap was purchased from Zhongjie Sifang Biotechnology Co., Ltd., with product batch number 20240325; Monochamus alternatus attractant was purchased from Zhongjie Sifang Biotechnology Co., Ltd., with product batch number 20225041; Y1 type spruce flower Monochamus alternatus attractant was purchased from Hangzhou Pheromone Biotechnology Co., Ltd., with product batch number 20240410; Monochamus alternatus attractant core ZL002 was purchased from Shenyang Zhonglin Biotechnology Co., Ltd., with product batch number 20240522.
[0024] In the following embodiments, unless otherwise specified, all of them are conventional methods.
[0025] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.
[0026] Example 1
[0027] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 1.0 parts of (1R)-(+)-alpha-pinene, 4.0 parts of 3-methylpentane, 2.0 parts of 2-undecanyloxy-1-ethanol, and 93.0 parts of paraffin oil.
[0028] Preparation method: The raw materials are mixed in proportion, stirred at 120 rpm for 3 min at room temperature, and the raw materials are mixed evenly and packed into a dark brown polyethylene sustained-release bag.
[0029] Example 2
[0030] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 2.0 parts of (1R)-(+)-alpha-pinene, 6.0 parts of 3-methylpentane, 4.0 parts of 2-undecanyloxy-1-ethanol, and 88.0 parts of paraffin oil.
[0031] Preparation method: The raw materials are mixed in proportion, stirred at 80 rpm for 8 min at room temperature, and the raw materials are mixed evenly and packed into a dark brown polyethylene sustained-release bag.
[0032] Example 3
[0033] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 1.2 parts of (1R)-(+)-alpha-pinene, 5.5 parts of 3-methylpentane, 2.2 parts of 2-undecanyloxy-1-ethanol, and 91.1 parts of paraffin oil.
[0034] Preparation method: The raw materials are mixed in proportion, stirred at 90 rpm for 5 min at room temperature, and the raw materials are mixed evenly and packed into a dark brown polyethylene sustained-release bag.
[0035] Example 4
[0036] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 1.5 parts of (1R)-(+)-alpha-pinene, 5.0 parts of 3-methylpentane, 3.5 parts of 2-undecanyloxy-1-ethanol, and 90.0 parts of paraffin oil.
[0037] Preparation method: The raw materials are mixed in proportion, stirred at 100 rpm for 5 min at room temperature, and the raw materials are mixed evenly and packed into a dark brown polyethylene sustained-release bag.
[0038] Example 5
[0039] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 1.3 parts of (1R)-(+)-alpha-pinene, 5.2 parts of 3-methylpentane, 2.5 parts of 2-undecanyloxy-1-ethanol, and 91.0 parts of paraffin oil.
[0040] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.1 mol·L -1 2-Undecyloxy-1-ethanol, 0.4 mol·L -1 3-Methylpentane, 0.08 mol·L -1 (1R)-(+)-α-pinene (paraffin oil as solvent).
[0041] Comparative Example 1
[0042] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 0.8 parts of (1S)-(-)-β-pinene, 6.2 parts of 2-undecanyloxy-1-ethanol, and 93.0 parts of ethanol.
[0043] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.25 mol·L -1 2-Undecyloxy-1-ethanol, 0.05 mol·L -1(1S)-(-)-β-pinene (ethanol as solvent).
[0044] Comparative Example 2
[0045] A spruce flower monochamus attractant is prepared from the following raw materials by volume: 1.5 parts of (1R)-(+)-camphor, 2.5 parts of 2-undecanyloxy-1-ethanol, and 96.0 parts of ethanol.
[0046] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.1 mol·L -1 2-Undecyloxy-1-ethanol, 0.1 mol·L -1 (1R)-(+)-Camphor (ethanol as solvent).
[0047] Comparative Example 3
[0048] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 1.3 parts of (1R)-(+)-alpha-pinene, 5.2 parts of 3-methylpentane, 2.5 parts of 2-undecanyloxy-1-ethanol, and 91.0 parts of ethanol.
[0049] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.1 mol·L -1 2-Undecyloxy-1-ethanol, 0.4 mol·L -1 3-Methylpentane, 0.08 mol·L -1 (1R)-(+)-α-pinene (ethanol as solvent).
[0050] Comparative Example 4
[0051] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 0.8 parts of (1S)-(-)-alpha-pinene, 6.2 parts of 2-undecanyloxy-1-ethanol, and 93.0 parts of paraffin oil.
[0052] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.25 mol·L -1 2-Undecyloxy-1-ethanol, 0.05 mol·L -1 (1S)-(-)-pinene (paraffin oil is the solvent).
[0053] Comparative Example 5
[0054] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 0.8 parts of (1S)-(-)-β-pinene, 6.2 parts of 2-undecanyloxy-1-ethanol, and 93.0 parts of paraffin oil.
[0055] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.25 mol·L -1 2-Undecyloxy-1-ethanol, 0.05 mol·L -1 (1S)-(-)-pinene (paraffin oil as solvent).
[0056] Comparative Example 6
[0057] A spruce flower monochamus attractant is prepared from the following raw materials by volume: 1.5 parts of (1R)-(+)-camphor, 2.5 parts of 2-undecanyloxy-1-ethanol, and 96.0 parts of paraffin oil.
[0058] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.1 mol·L -1 2-Undecyloxy-1-ethanol, 0.1 mol·L -1 (1R)-(+)-Camphor (paraffin oil as solvent).
[0059] Comparative Example 7
[0060] A spruce-flowered Monochamus alternatus attractant is prepared from the following raw materials, measured by volume: 1.3 parts of (1S)-(-)-β-pinene, 5.2 parts of 3-methylpentane, 2.5 parts of 2-undecanyloxy-1-ethanol, and 91.0 parts of paraffin oil.
[0061] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.1 mol·L -1 2-Undecyloxy-1-ethanol, 0.4 mol·L -1 3-Methylpentane, 0.08 mol·L -1 (1S)-(-)-β-pinene (paraffin oil as solvent).
[0062] Comparative Example 8
[0063] A spruce flower monochamus attractant is prepared from the following raw materials by volume: 1.3 parts of (1R)-(+)-alpha-pinene, 1.5 parts of camphor, 2.5 parts of 2-undecanyloxy-1-ethanol and 94.7 parts of paraffin oil.
[0064] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.1 mol·L -1 2-Undecyloxy-1-ethanol, 0.1 mol·L -1 Camphor, 0.08 mol·L -1 (1R)-(+)-α-pinene (ethanol as solvent).
[0065] Comparative Example 9
[0066] A spruce flower monochamus attractant is prepared from the following raw materials by volume: 1.3 parts of (1R)-(+)-alpha-pinene, 5.2 parts of 3-methylpentane, 1.5 parts of camphor and 92.0 parts of paraffin oil.
[0067] Preparation method: Mix the raw materials in proportion, stir at 90 rpm for 5 minutes at room temperature, mix the raw materials evenly, and put them into a dark brown polyethylene sustained-release bag. The concentration of the active ingredient in the spruce-flowered Monochamus alternatus attractant is: 0.1 mol·L -1 Camphor, 0.4 mol·L -1 3-Methylpentane, 0.08 mol·L -1 (1R)-(+)-α-pinene (ethanol as solvent).
[0068] Test Example 1
[0069] Indoor Y-tube competitive test experiment:
[0070] The olfactory behavior was measured using a Y-type olfactometer in a dark room. The behavior selection of male adults, unmated female adults, and female adults in the egg-laying period for the compound solution was measured. The compound combination of the present invention ((1S)-(-)-pinene 0.8 parts, 2-undecyloxy-1-ethanol 6.2 parts, paraffin oil 93.0 parts, recorded as the experimental group) was used, and existing commercial products (Y1-type spruce flower Monochamus alternatus attractant, Monochamus alternatus attractant core ZL002, Monochamus alternatus attractant) were used as controls.
[0071] Select 3 naturally emerged adults of Monochamus alternatus and use Korean pine branches to raise them individually in insect boxes indoors. Take out the branches 12 hours before the test to keep the test insects hungry. The main arm of the Y-shaped tube is 50 cm long and has an inner diameter of 4.5 cm; the arms on both sides are 35 cm long and have an inner diameter of 4.5 cm, and the angle between the arms on both sides is 75°. Use a silicone tube to connect the LCA-3 air generator (output 0-3 L·min -1 ), the triangular gas washing bottle, the gas flow meter, the spherical trap and the Y-tube are connected, and the air flow rate is adjusted to 500 mL min -1 . During the test, 10 μL of the experimental group and control group solutions were taken with a pipette and dropped onto two 30 mm × 5 mm qualitative filter papers respectively. The filter paper strips were placed in the spherical traps of the two selection arms of the Y-shaped tube. During the measurement, the adults were introduced from the mouth of the main arm of the Y-shaped tube, and the timing began after the adult longhorn beetles were stabilized. Each time one adult was released to test the behavioral response, and the observation time was 5 to 10 minutes. The test insects that entered the selection arm and moved a distance exceeding 1 / 2 of the length of the selection arm and stayed for more than 30 seconds were recorded as having made a choice, otherwise it was recorded as having no choice. Adults that did not make a choice within 5 minutes were recorded as not responding. After testing each test insect, the direction of the odor arm and the control arm was changed. After each group (except for the control group of ZL002 bait cores for female insects in the egg-laying period, the other groups were all 30 insects in one group), the inner wall of the Y-shaped tube was wiped with 75% ethanol, rinsed with distilled water, and then dried with a hair dryer; when changing different odor sources, the inner wall of the Y-shaped tube was wiped with 75% ethanol, rinsed with distilled water, and then placed in an oven at 120℃ for 2h. Each group of 10 longhorn beetles was repeated 3 times. The number of insects in each group was counted, and the results are shown in Table 1.
[0072] Table 1 Number of Monochamus alternatus
[0073]
[0074] Note: ns means no significant difference, * means significant difference.
[0075] As can be seen from Table 1, for male insects, the attractant of the present invention, the Y1-type spruce-flowered Monochamus alternatus attractant, and the Monochamus alternatus attractant core ZL002 have a comparable number of spruce-flowered Monochamus alternatus attracted, and the effect is significantly better than the attractant of the genus Monochamus alternatus; for unmated female insects, the attractant of the present invention, the Y1-type spruce-flowered Monochamus alternatus attractant, and the Monochamus alternatus attractant have a comparable number of spruce-flowered Monochamus alternatus attracted, and the effect is slightly worse than the Monochamus alternatus attractant core ZL002; for female insects in the egg-laying period, the attractant of the present invention, the Y1-type spruce-flowered Monochamus alternatus attractant, the Monochamus alternatus attractant core ZL002, and the Monochamus alternatus attractant have a comparable number of spruce-flowered Monochamus alternatus attracted.
[0076] Test Example 2
[0077] From June 20 to August 3, 2024, the trapping and killing effects of attractants were tested in pure Korean pine forests, pure Chinese pine forests, pure Scots pine forests, and Korean pine-larch mixed forests (6:4 mixed) in Wendao Forest Farm, Fushun City, Liaoning Province. The experiments used the attractants of Example 5 and Comparative Examples 1 to 9 (dosage was 10 mL / group) and 3 commercial controls (Monochromis alternatus attractant, dosage was 1 unit; Y1 type spruce flower Monochromis alternatus attractant, dosage was 180 mL / group; Monochromis alternatus attractant core ZL002, dosage was 1 unit), and each attractant was set up with 4 groups of repetitions, and each attractant in the group was randomly arranged. Record, collect, and take out once every 3 days, and count the sex and number of spruce flower Monochromis alternatus adults trapped. The experimental results of Korean pine forest, Chinese pine forest, Scots pine forest, and Korean pine-Larch mixed forest (6:4 mixed) were similar. The results of the four forest types were analyzed and statistically analyzed, and the results are shown in Table 2.
[0078] Table 2 Sex and number of adults of Monochamus alternatus in different treatment groups
[0079]
[0080]
[0081] In Table 2, the highest standard of data volume is recorded as a, which is higher than b, significantly higher than c and d, b is significantly higher than c and d, and c is significantly higher than d. As can be seen from Table 2, the number of spruce beetles killed by the attractant corresponding to Example 5 is significantly higher than that of the attractants of Comparative Examples 1 to 9 and the existing commercial Y1-type spruce beetle attractant and the Monochamus alternatus attractant ZL002, with significant differences (P < 0.05). At the same time, the attractant of Example 5 has a better killing effect than the Monochamus alternatus attractant. This shows that the attractant of the present invention has a significant effect of killing spruce beetles, and the effect is better than that of existing commercial products.
[0082] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A spruce beetle attractant, characterized in that: The invention is prepared from the following raw materials by volume: 1-2 parts of (1R)-(+)-α-pinene, 4-6 parts of 3-methylpentane, 2-4 parts of 2-undecyloxy-1-ethanol and 88-93 parts of paraffin oil.
2. The method for preparing the Monochamus alternatus attractant according to claim 1, characterized in that: The following steps are involved: The raw materials are mixed according to proportion, stirred evenly and packed into polyethylene slow-release bags.
3. The preparation method according to claim 2, characterized in that: The stirring speed is 80-120 rpm, and the stirring time is 2-8 min.
4. The preparation method according to claim 2, characterized in that: The polyethylene sustained-release bag is a dark brown polyethylene sustained-release bag.
5. Use of the attractant for Monochamus alternatus according to claim 1 or the preparation method according to any one of claims 2 to 4 in preparing products for killing Monochamus alternatus.
6. A product for luring and killing Monochamus alternatus, characterized in that: It comprises a spruce-flowered Monochamus alternatus attractant and a beetle trapping device; the spruce-flowered Monochamus alternatus attractant is the spruce-flowered Monochamus alternatus attractant described in claim 1 or the spruce-flowered Monochamus alternatus attractant prepared by the preparation method described in any one of claims 2 to 4.
7. A method for luring and killing Monochamus alternatus in the forest, characterized in that: Placing the product of claim 6 in the woods.
8. The method according to claim 7, characterized in that The dosage of the product is 8-15 mL / group, 1-2 groups / hm 2 .
9. The method according to claim 7, characterized in that: The forests include red pine forests, Chinese pine forests, larch forests, and larch forests.