A mosquito repellent composition based on pandan leaf extract

Abstract

The present invention discloses a mosquito repellent composition based on pandan leaf extract, belonging to the field of mosquito repellent technology. The raw materials of the mosquito repellent composition based on pandan leaf extract of the present invention are measured by mass, including: 15-27 parts of pandan leaf extract, 4-8 parts of geranium extract, 2-5 parts of lavender extract, 1-3 parts of mint extract, 10-15 parts of grape seed oil, and 40-70 parts of solvent. The present invention achieves an excellent mosquito repellent effect through the joint action of multiple plant extracts with mosquito repellent effects. The pandan leaf extract prepared by the present invention is rich in active ingredients such as flavonoids and volatile oils. The odor released by the extract can effectively repel mosquitoes, affect the normal physiological activities of mosquitoes, and has obvious repellent activity.

Description

Technical Field

[0001] The present invention relates to the technical field of mosquito repellent, in particular to a mosquito repellent composition based on pandan leaf extract. Background Art

[0002] Mosquitoes are small insects of the family Culicidae in the order Diptera and are found worldwide. For years, the problem of mosquitoes easily breeding in conditions such as high temperatures and humidity has remained unresolved, continuously impacting human life. High levels of mosquito activity also increase the risk of spreading infectious diseases such as malaria, Japanese encephalitis, and dengue fever, posing a threat to human health.

[0003] As people's living standards continue to improve, the question of how to effectively prevent and repel mosquitoes has gradually attracted more attention. Existing mainstream mosquito repellent products, such as floral water, essential oils, medicated oil, and mosquito coils, while effective in repelling mosquitoes, generally suffer from issues such as high irritation, poor durability, and some of their raw materials being toxic or even carcinogenic to humans. Furthermore, these mainstream mosquito repellent products primarily rely on active ingredients such as camphor, pyrethroids, pralin, pyrethrin, and citronellal to achieve their repellent effects, but these ingredients can easily cause mosquitoes to develop drug resistance. Therefore, there is an urgent need for researchers in this field to design a new mosquito repellent product that is less irritating, more durable, and non-toxic.

[0004] Pandan leaves, also known as fragrant pandanus and colorful leaves, are perennial tropical herbs known for their unique aroma. Their fresh, elegant fragrance is highly popular with the public and relatively affordable. Recent research has also shown that the active ingredients in pandan leaves have a certain insect repellent effect. Therefore, the design of a mosquito repellent product using pandan leaves is of great research value. Summary of the Invention

[0005] The present invention aims to provide a mosquito repellent composition based on a pandan leaf extract to address the aforementioned issues in the prior art. The present invention achieves excellent mosquito repellent efficacy through the synergistic action of multiple plant extracts with mosquito repellent properties. The pandan leaf extract prepared in the present invention is rich in active ingredients such as flavonoids and volatile oils. The odor released by the extract effectively repels mosquitoes, affecting their normal physiological activities and exhibiting significant repellent activity.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] One of the technical solutions of the present invention is to provide a mosquito repellent composition based on pandan leaf extract, the raw materials, calculated by weight, comprising:

[0008] 15-27 parts of pandan leaf extract, 4-8 parts of geranium extract, 2-5 parts of lavender extract, 1-3 parts of peppermint extract, 10-15 parts of grape seed oil and 40-70 parts of solvent.

[0009] Preferably, the preparation method of the pandan leaf extract is supercritical carbon dioxide extraction.

[0010] Preferably, the supercritical carbon dioxide extraction comprises the following steps:

[0011] The pandan leaves are crushed until they pass through a 25-80 mesh sieve, and then placed in an extraction kettle of a supercritical carbon dioxide extractor for cyclic extraction. The raw material system is then passed into a separation kettle for collection to obtain a pandan leaf extract.

[0012] Preferably, the conditions of the cyclic extraction are: extraction pressure of 15-20 MPa, extraction temperature of 40-50° C., entrainer of 70-85 vol% ethanol aqueous solution, and carbon dioxide flow rate of 10-15 L / min.

[0013] Preferably, the raw materials are calculated by mass as follows:

[0014] 26 parts of pandan leaf extract, 7 parts of geranium extract, 3 parts of lavender extract, 2 parts of peppermint extract, 15 parts of grape seed oil and 60 parts of solvent.

[0015] Preferably, the solvent is ethanol.

[0016] The second technical solution of the present invention is to provide a method for preparing the above-mentioned mosquito repellent composition based on pandan leaf extract, comprising the following steps:

[0017] The raw materials are mixed to obtain the mosquito repellent composition based on the pandan leaf extract.

[0018] The third technical solution of the present invention: provides an application of the above-mentioned mosquito repellent composition based on pandan leaf extract in the preparation of products with mosquito repellent effect.

[0019] The present invention increases the content of active substances in the raw materials by controlling the temperature, pressure and other conditions of the extraction process. By improving the extraction process, the mosquito repellent composition of the present invention can achieve a higher mosquito repellent effect.

[0020] The beneficial technical effects of the present invention are as follows:

[0021] The present invention achieves excellent mosquito repellent effects through the synergistic action of multiple plant extracts with mosquito repellent properties. The pandan leaf extract prepared in the present invention is rich in active ingredients such as flavonoids and volatile oils. The odor released by the extract effectively repels mosquitoes, affecting their normal physiological activities and exhibiting significant repellent activity.

[0022] In addition, the mosquito repellent composition prepared by the present invention retains a large amount of linoleic acid, which is mainly derived from pandan leaves, and can improve the storage stability of the mosquito repellent composition, so that its mosquito repellent effect is retained for a longer time, and the mosquito repellent effect is achieved by working together with other ingredients in the composition.

[0023] The present invention has found through experimental testing that the addition of geranium extract can greatly improve the odor acceptance of the product, make the fragrance more natural, and reduce the odor irritation.

[0024] The extraction process designed in the present invention is simple and easy to operate, requiring minimal equipment. The formulation uses natural raw materials and does not contain chemical ingredients such as pyrethroids and DEET, making it safe and non-toxic. It also has a light, non-irritating odor and excellent application value. Furthermore, the mosquito repellent composition produced in the present invention can soothe nerves and improve sleep. DETAILED DESCRIPTION

[0025] Various exemplary embodiments of the present invention are now described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terms used in the present invention are only for describing specific embodiments and are not intended to limit the present invention.

[0026] In addition, for numerical ranges in the present invention, it is understood that each intervening value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between any stated value or stated range, and any other stated value or intervening value in the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention.

[0028] The terms “include,” “including,” “have,” “contain,” etc. used in the present invention are open-ended terms, meaning including but not limited to.

[0029] The raw materials used in the following examples and comparative examples of the present invention are all commercially available products.

[0030] Example 1

[0031] A mosquito repellent composition, comprising the following raw materials in parts by weight:

[0032] 26 parts of pandan leaf extract, 7 parts of geranium extract, 3 parts of lavender extract, 2 parts of peppermint extract, 15 parts of grape seed oil, and 60 parts of ethanol (95 vol%).

[0033] The preparation method of pandan leaf extract is as follows: the pandan leaves are washed, placed in a 40°C oven to dry to constant weight, crushed to pass through a 25-mesh sieve, and then placed in an extraction kettle of a supercritical carbon dioxide extractor for cyclic extraction; the conditions of the cyclic extraction are: extraction pressure of 20 MPa, extraction temperature of 45°C, entrainer of 200 mL of 80 vol% ethanol aqueous solution, and carbon dioxide flow rate of 13 L / min; after the extraction is completed, the raw material system is passed into a separation kettle at 25°C, collected, and the pandan leaf extract is obtained.

[0034] The preparation method of the geranium extract comprises the following steps: washing geranium flowers, drying them in an oven at 40° C. to constant weight, then crushing them until they pass through a 25-mesh sieve, placing them in a sample bottle, mixing them with a 60 vol % ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, extracting them at a temperature of 25° C. and an ultrasonic frequency of 30 kHz for 40 minutes, and then concentrating under reduced pressure to remove the solvent to obtain the geranium extract.

[0035] The preparation method of lavender extract comprises the following steps: washing lavender, drying the lavender in a 40°C oven to constant weight, crushing the lavender until it passes through a 25-mesh sieve, placing the lavender in a sample bottle, mixing the lavender with a 60 vol% ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, extracting the lavender at a temperature of 25°C and an ultrasonic frequency of 30 kHz for 40 minutes, and then concentrating the lavender under reduced pressure to remove the solvent to obtain the lavender extract.

[0036] The preparation method of mint extract comprises the following steps: washing mint leaves, drying them in an oven at 40° C. to constant weight, then crushing them until they pass through a 25-mesh sieve, placing them in a sample bottle, mixing them with a 60 vol % ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, extracting them at a temperature of 25° C. and an ultrasonic frequency of 30 kHz for 40 minutes, and then concentrating under reduced pressure to remove the solvent to obtain the mint extract.

[0037] Preparation of mosquito repellent composition:

[0038] The pandan leaf extract, geranium extract, lavender extract, mint extract, grape seed oil and ethanol are mixed to obtain a mosquito repellent composition.

[0039] Example 2

[0040] The only difference from Example 1 is that it consists of the following raw materials in parts by mass:

[0041] 17 parts of pandan leaf extract, 4 parts of geranium extract, 5 parts of lavender extract, 1 part of mint extract, 10 parts of grape seed oil, and 40 parts of ethanol (95 vol%).

[0042] Comparative Example 1

[0043] The only difference from Example 1 is that the type of entrainer in the process of preparing the pandan leaf extract is changed to an equal volume of 80 vol% methanol aqueous solution.

[0044] Comparative Example 2

[0045] The only difference from Example 1 is that the preparation method of the pandan leaf extract is modified as follows:

[0046] The pandan leaves were washed, dried in an oven at 40°C to constant weight, then crushed to pass through a 25-mesh sieve, placed in a sample bottle, mixed with a 60 vol% ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, extracted at a temperature of 25°C and an ultrasonic frequency of 30 kHz for 40 min, and then concentrated under reduced pressure to remove the solvent to obtain a pandan leaf extract.

[0047] Comparative Example 3

[0048] The difference from Example 1 is only that the pandan leaves are replaced with mugwort leaves of equal mass.

[0049] Comparative Example 4

[0050] The only difference from Example 1 is that the addition of geranium extract, lavender extract and mint extract is omitted and an equal mass of pandan leaf extract is added.

[0051] Comparative Example 5

[0052] The only difference from Example 1 is that the addition of geranium extract is omitted and an equal mass of pandan leaf extract is added.

[0053] Comparative Example 6

[0054] The only difference from Example 1 is that the addition of grape seed oil is omitted and an equal mass of ethanol (95 vol%) is added.

[0055] Effect verification

[0056] (1) A mosquito repellent test was conducted in accordance with GB / T 13917.9-2009. A total of 16 test subjects were tested by applying the above mosquito repellent composition (application volume 1.5 μL / cm 2 The repellent effect against Aedes albopictus was tested by using a PCR method, and the effective protection time was calculated. The test results are shown in Table 1.

[0057] Table 1

[0058]

[0059]

[0060] (2) The fragrance retention effect, fragrance acceptance, fragrance irritation, and storage stability of the mosquito repellent composition were tested. The test results are shown in Table 2.

[0061] The fragrance retention test conditions were as follows: 2 mL of the mosquito repellent composition was dripped onto a sterile cotton ball (0.3 g) and then left in an open environment to simulate the fragrance retention of the mosquito repellent composition on clothing. After 10 hours, 10 testers were selected to score the fragrance retention, and the test results were averaged. The scoring criteria are shown in Table 2. The test results are shown in Table 3.

[0062] The test conditions for fragrance acceptance and fragrance irritation were as follows: 2 mL of the above mosquito repellent composition was dropped onto a sterile cotton ball (0.3 g), then left to stand in an open environment. After 30 minutes, 10 testers were selected to rate the fragrance acceptance and fragrance irritation, and the test results were averaged. The scoring criteria are shown in Table 2. The test results are shown in Table 3.

[0063] The storage stability test conditions are as follows: 50 mL of the above mosquito repellent composition was placed in a closed environment for 7 days, and the product system was observed to see if there was any stratification. The test results are shown in Table 3.

[0064] Table 2

[0065]

[0066] Table 3

[0067] Long-lasting fragrance effect Fragrance acceptance Fragrance irritation Storage stability Example 1 9 9 10 No stratification Comparative Example 1 8 9 9 No stratification Comparative Example 2 8 7 8 No stratification Comparative Example 3 7 6 4 No stratification Comparative Example 4 5 5 6 No stratification Comparative Example 5 6 5 6 No stratification Comparative Example 6 4 8 5 No stratification

[0068] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A mosquito repellent composition based on pandan leaf extract, characterized in that: Raw materials, calculated by mass, include: 15-27 parts of pandan leaf extract, 4-8 parts of geranium extract, 2-5 parts of lavender extract, 1-3 parts of peppermint extract, 10-15 parts of grape seed oil and 40-70 parts of solvent; The preparation method of the pandan leaf extract comprises: washing the pandan leaves, drying them in a 40°C oven to constant weight, crushing them until they pass through a 25-mesh sieve, and then placing them in an extraction kettle of a supercritical carbon dioxide extractor for cyclic extraction; the cyclic extraction conditions are: extraction pressure of 20 MPa, extraction temperature of 45°C, entrainer of 200 mL of 80 vol% ethanol aqueous solution, and carbon dioxide flow rate of 13 L / min; after the extraction is completed, passing the raw material system into a separation kettle at 25°C for collection to obtain the pandan leaf extract; The preparation method of the geranium extract comprises: washing geranium flowers, drying them in a 40° C. oven to constant weight, then crushing them until they pass through a 25-mesh sieve, placing them in a sample bottle, mixing them with a 60 vol% ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, extracting them at a temperature of 25° C. and an ultrasonic frequency of 30 kHz for 40 minutes, and then concentrating under reduced pressure to remove the solvent to obtain the geranium extract; The preparation method of the lavender extract comprises: washing the lavender, placing it in a 40° C. oven to dry to constant weight, then crushing it until it passes through a 25-mesh sieve, placing it in a sample bottle, mixing it with a 60 vol% ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, extracting it at a temperature of 25° C. and an ultrasonic frequency of 30 kHz for 40 minutes, and then concentrating under reduced pressure to remove the solvent to obtain the lavender extract; The preparation method of the mint extract comprises: washing mint leaves, drying them in a 40° C. oven to constant weight, then crushing them until they pass through a 25-mesh sieve, placing them in a sample bottle, mixing them with a 60 vol% ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, extracting them at a temperature of 25° C. and an ultrasonic frequency of 30 kHz for 40 minutes, and then concentrating under reduced pressure to remove the solvent to obtain the mint extract; The solvent is ethanol.

2. The mosquito repellent composition based on pandan leaf extract according to claim 1, characterized in that The raw materials are calculated by mass as follows: 26 parts of pandan leaf extract, 7 parts of geranium extract, 3 parts of lavender extract, 2 parts of peppermint extract, 15 parts of grape seed oil and 60 parts of solvent.

3. A method for preparing the mosquito repellent composition based on the pandan leaf extract according to any one of claims 1-2, characterized in that: The following steps are involved: The raw materials are mixed to obtain the mosquito repellent composition based on the pandan leaf extract.

4. Use of the mosquito repellent composition based on the pandan leaf extract according to any one of claims 1 to 2 in the preparation of a product with mosquito repellent effect.

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