Probe, primer, kit and method for detecting alpha-globin gene

CN119464464APending Publication Date: 2025-02-18SHANGHAI HORIZON MEDICAL SCI CO LTD +1
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Patent Information

Application Number
CN202311016360.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-08-11
Publication Date
2025-02-18

AI Technical Summary

Technical Problem

During the mutation detection process of the traditional α-thalassemia detection products for six common α-globin genes, primers and probes consume a lot, the reaction system is complex, and the experimental process is cumbersome, resulting in high cost and long time.

Method used

A probe and primer were designed. Through the optimization design of primer probes, only 3 pairs of primers matched with 4 probes can be used to distinguish between the three dissolution curve peaks of wild type, homozygous mutant type and heterozygous type. Simplify the reaction system, reduce the use of reagents, and improve detection efficiency.

Benefits of technology

It realizes efficient detection of α-globin genes, simplifies the experimental process, reduces costs, shortens detection time, and has stable performance.

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Abstract

The invention relates to a probe, a primer, a kit and a method for detecting an alpha-globin gene. The probe provided by the invention is selected from one or more of the following probes P1 to P4: the probe P1 has a sequence as shown in SEQ ID NO.10; the sequence of the probe P2 is as shown in SEQ ID NO. 11; the sequence of the probe P3 is as shown in SEQ ID NO. 12; the sequence of the probe P4 is as shown in SEQ ID NO. 13. The technical scheme for detecting the genotype of the alpha-globin gene is simple and low in cost.
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