Lettuce genetic engineering genetic research culture method and equipment

Abstract

The invention belongs to the technical field of lettuce gene research, and particularly relates to a lettuce genetic engineering genetic research culture method and equipment, the culture equipment comprises an incubator and a culture dish, the culture dish is placed in a culture area in the incubator, and the incubator is used for ensuring normal growth of lettuce samples in the culture dish; the culture box comprises a box body, a temperature and humidity module, a ventilation module and a disinfection module; the ventilation module is used for realizing air exchange between the culture area and the external environment; by arranging the ventilation module, normal exchange and circulation of air in the culture area are guaranteed, meanwhile, the flowing-in air is purified, impurities, bacteria and other factors possibly harming normal growth of lettuce samples in the air are removed, air purification in the culture area is achieved, and the normal growth environment of the lettuce samples is improved; the smooth proceeding of lettuce gene engineering research is ensured.

Description

Technical Field

[0001] The invention belongs to the technical field of lettuce gene research, in particular to a method and equipment for genetic engineering genetic research and cultivation of lettuce. Background Art

[0002] With the advancement of technology, genetic transformation methods have been integrated into vegetable transformation projects. For example, lettuce has many varieties, a short growth cycle, and can be grown through hydroponics. It is one of the most common green leafy vegetables. Since lettuce has a wide range of uses and a large demand, the genetic improvement of lettuce and the acceleration of the lettuce hydroponics process will inevitably become the strongest demand for green vegetables in today's society. Therefore, in the genetic transformation genetic engineering of lettuce, the research on genetic transformation of lettuce was carried out earlier and has a better genetic transformation system.

[0003] In the field of modern biological research and plant tissue culture, tissue culture instruments and equipment play a vital role. A complete set of tissue culture equipment can ensure the accuracy and repeatability of the experimental process, improve research efficiency, and provide a stable and reliable working environment for scientific researchers.

[0004] Among them, the incubator is the core equipment in the tissue culture experiment, which is used to provide constant temperature, humidity and light conditions to support the growth and development of plant tissues, cells or seedlings; during the culture process, if the incubator is opened and closed too many times, it is easy for debris and bacteria in the air to invade the lettuce sample culture dish and cause adverse effects, thus hindering the progress of research work; but if it is kept closed, the carbon dioxide concentration is likely to increase during the growth of the lettuce samples, resulting in the stunted development of the lettuce samples and affecting the normal progress of the research work. Summary of the invention

[0005] In order to make up for the deficiencies of the prior art and solve the above-mentioned technical problems, the present invention proposes a method and equipment for genetic engineering research and cultivation of lettuce.

[0006] The technical solution adopted by the present invention to solve the technical problem is as follows: the present invention proposes a lettuce genetic engineering genetic research and cultivation device, comprising a culture box and a culture dish, the culture dish is placed in the culture area inside the culture box, the culture box is used to ensure the normal growth of the lettuce sample inside the culture dish; the culture box comprises a box body, a temperature and humidity module, a ventilation module and a disinfection module, the ventilation module is used to realize the air exchange between the culture area and the external environment; The ventilation module comprises a ventilation port arranged at the top of the box body, a ventilation cavity is arranged at the top of the box body located above the culture area, a purification cylinder is arranged inside the ventilation cavity, closing plates are arranged at both ends of the purification cylinder, an inner ring and an outer ring are arranged between the closing plates at both sides, the inner ring is located in the middle of the area surrounded by the outer ring, and both the inner ring and the outer ring are mesh structures; The gap area between the inner ring and the outer ring is a purification area, which is filled with purification fillers. The area surrounded by the inner ring is a ventilation area, which is connected to the ventilation port through a connecting pipe. The bottom of the purification area is communicated with the incubator, and an air outlet is arranged at the bottom of the incubator, and the air outlet is also communicated with the inside of the incubation area.

[0007] Preferably, a disinfection channel is provided at the top of the outer ring, the disinfection channel extends upward to the top of the ventilation area, and a disinfection lamp is provided on the lower surface of the top of the ventilation area in the area surrounded by the disinfection channel; The inner ring is rotatably connected to the closing plate, and a rotating device is provided at a position of the closing plate corresponding to the inner ring to drive the inner ring to rotate relative to the outer ring; The outer surface of the inner ring is evenly provided with toggle blocks, and the ends of the toggle blocks are bent.

[0008] Preferably, a dust collecting bin is provided on the back side of the closing plate away from the ventilation port, and the dust collecting bin is communicated with the interior of the ventilation zone through a connecting hole, an air duct is provided at the end of the dust collecting bin, the air duct connects the ventilation zone and the internal area of ​​the dust collecting bin, and an intercepting net is provided on the inner wall of the dust collecting bin between the air duct and the connecting hole.

[0009] Preferably, an extrusion ring is provided on one side of the purification zone, and the extrusion ring is slidably connected to the inner wall of the purification zone; a pushing device is provided on the side wall of the ventilation zone, and the output end of the pushing device slides through the corresponding closing plate and is connected to the extrusion ring.

[0010] Preferably, combing rods are annularly distributed on the surface of the extrusion ring, the combing rods extend transversely inside the purification area, and combing blocks are arranged in the areas of the combing rods located in the gaps between the toggle blocks.

[0011] Preferably, the combing rod on the extrusion ring is rotatably connected, and a torsion spring is provided at the rotatably connected position; and a vibrator is provided inside the extrusion ring.

[0012] Preferably, the end of the air guide tube is introduced into the gap inside the ventilation area between the closing plate and the extrusion ring; the combing rod is a tubular structure, and the end of the combing tube is communicated with the gap area between the closing plate and the extrusion ring; impact holes are evenly arranged on the end of the combing block, and the impact holes are communicated with the interior of the combing rod.

[0013] Preferably, compressed balls are mixed in the gaps between the purification fillers, and the compressed balls are hollow inside and are made of elastic material.

[0014] Preferably, through holes are evenly arranged on the surface of the compressed ball, and the compressed ball is filled with dry particles, and the particle size of the dry particles is larger than the aperture of the through holes.

[0015] A method for genetic engineering genetic research and cultivation of lettuce, the method using the above-mentioned cultivation equipment, the specific steps of the method are: S1: Select lettuce seeds for sterile cleaning, use filter paper to absorb the moisture of the lettuce seeds, and inoculate them into a special culture dish for germination, and place the special culture dish for germination in the culture area inside the incubator for cultivation; S2: Through the display interface and control buttons of the LCD screen outside the box, the operator can adjust the temperature and humidity module and the ventilation module to adjust the temperature and humidity of the internal environment of the culture area and the concentration of carbon dioxide in the air, so that the lettuce seed samples in the culture dish can germinate and grow normally; S3: taking out the germinated lettuce seeds, cutting off part of the cotyledons as explants, and inoculating them with the leaf surface facing downward into a culture medium prepared in advance in a culture dish for pre-culture; using an infection solution to infect the explants, and then preparing a callus induction medium to perform callus induction on the explant samples to induce the formation of explant callus tissue; S4: Callus screening is performed on the formed explants and callus tissues through callus screening medium, so that qualified samples are screened out and cultured into lettuce seedlings, and observations and records are made after development, and the recorded data are compared and analyzed.

[0016] The beneficial effects of the present invention are as follows: The method and equipment for genetic engineering research and cultivation of lettuce of the present invention are provided with a ventilation module to ensure normal exchange and circulation of air inside the cultivation area, and to purify the inflowing air to remove factors such as foreign matter and bacteria that may harm the normal growth of the lettuce sample; By starting the circulating fan located inside the connecting pipe, the outside air is introduced into the connecting pipe, so that the air penetrates from the inside to the outside and passes through the purification area in the gap between the inner ring and the outer ring, and the impurities and dust in the air are filtered out. At the same time, because the purification filler filled in the purification area uses the type of existing antibacterial particles, such as nano silver antibacterial particles or antibacterial plastic particles, etc.; Therefore, when the air passes through the gaps in the purification filler, pollutants such as bacteria in the air are retained in the purification filler and purified; the air inside the culture area is purified, the normal growth environment of the lettuce samples is improved, and the smooth progress of the genetic engineering research on lettuce is ensured. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present invention will be further described below in conjunction with the accompanying drawings.

[0018] Figure 1is a three-dimensional diagram of the culture device of the present invention; Figure 2 is a cross-sectional view of the culture device of the present invention; Figure 3 yes Figure 2 A partial enlarged view of the middle A; Figure 4 is a three-dimensional diagram of the purification cartridge of the present invention; Figure 5 is a stereogram of the extrusion ring of the present invention; Figure 6 is a cross-sectional view of a carding block in the present invention; Figure 7 is a cross-sectional view of a compression ball in the present invention; Figure 8 It is a flow chart of the culture method of the present invention.

[0019] In the figure: incubator 1, incubation area 11, box body 12, vent 121, ventilation cavity 13, air outlet 14, culture dish 2, purification cylinder 3, closing plate 31, inner ring 32, rotating device 321, toggle block 322, outer ring 33, disinfection channel 331, disinfection lamp 332, purification area 34, ventilation area 35, connecting pipe 351, dust collecting cylinder 36, connecting hole 361, air guide tube 362, intercepting net 363, extrusion ring 37, pushing device 371, combing rod 372, combing block 373, impact hole 374, compression ball 4. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Embodiment 1: In order to effectively solve the above problems, as shown in the accompanying drawings of the specification Figure 1-Figure 7As shown, a lettuce genetic engineering genetic research and cultivation equipment comprises a culture box 1 and a culture dish 2, the culture dish 2 is placed in a culture area 11 inside the culture box 1, and the culture box 1 is used to ensure the normal growth of the lettuce sample inside the culture dish 2; the culture box 1 comprises a box body 12, a temperature and humidity module, a ventilation module and a disinfection module, and the ventilation module is used to realize the air exchange between the culture area 11 and the external environment; the temperature and humidity module comprises an existing heating system, a refrigeration system and a humidification system, and a temperature and humidity sensor arranged inside the culture area 11, and the temperature and humidity data detected inside the culture area 11 are reflected on the display interface of the liquid crystal screen outside the box body 12, and the operator can also adjust the power of the heating system, the refrigeration system and the humidification system through the control button, so as to control the temperature and humidity inside the culture area 11, and meet the cultivation environment requirements of the lettuce sample; The ventilation module includes a ventilation port 121 arranged at the top of the box body 12, a ventilation cavity 13 is arranged at the top of the box body 12 in the upper area of ​​the culture area 11, a purification cylinder 3 is arranged inside the ventilation cavity 13, and closing plates 31 are arranged at both ends of the purification cylinder 3, and the closing plates 31 are circular plate structures; an inner ring 32 and an outer ring 33 are arranged between the closing plates 31 on both sides, both of which are cylindrical structures; the inner ring 32 is located in the middle of the area surrounded by the outer ring 33, and the inner ring 32 and the outer ring 33 are both prepared by using a metal filter structure; The gap area between the inner ring 32 and the outer ring 33 is the purification area 34, which is filled with purification fillers. The area surrounded by the inner ring 32 is the ventilation area 35, which is connected to the ventilation port 121 through the connecting pipe 351. One end of the connecting pipe 351 is connected to the ventilation port 121, and the other end extends to the inside of the ventilation area 35. The surface of the part of the connecting pipe 351 located inside the ventilation area 35 is evenly provided with holes, so that the air is input into the connecting pipe 351 under the action of the circulation fan and then flows smoothly into the ventilation area 35. The bottom of the purification area 34 is connected to the incubator 1, and an air outlet 14 is provided at the bottom of the incubator 1, and the air outlet 14 is also connected to the inside of the culture area 11; a circulating fan is provided inside the connecting pipe 351 and at the air outlet 14. Through the operation of the circulating fan, the air exchange between the inside of the culture area 11 and the external environment is controlled to avoid the accumulation of carbon dioxide in the culture area 11 affecting the growth of the lettuce samples, which is not conducive to the normal progress of the lettuce genetic engineering research.

[0022] Specific work flow: open the closed door on the front of the box 12, place the culture dish 2 with the lettuce sample on the mesh structure bracket inside the culture area 11, and then close the closed door; during the culture process, the number of times the closed door is opened and closed should be reduced as much as possible, so as to reduce the adverse effects of debris and bacteria flowing into the air during the opening and closing process on the lettuce sample culture dish 2, thereby hindering the progress of the research work; therefore, in order to ensure the smooth progress of the research, it is necessary to place the lettuce sample inside the culture area 11, control the surrounding environmental indicators such as temperature and humidity, and provide an ideal growth environment, so as to ensure that the final lettuce sample growth research data is sufficiently reliable; Furthermore, in order to ensure that the incoming air will not bring in a large amount of bacteria and debris from the external environment during the ventilation process, and reduce the probability of the culture dish 2 being contaminated, a ventilation module is provided to ensure that the air in the culture area 11 is normally exchanged and circulated, and at the same time, the incoming air is purified to remove debris, bacteria and other factors that may endanger the normal growth of the lettuce sample; specifically, the sensor provided inside the culture area 11 can detect the internal carbon dioxide concentration and oxygen concentration. When the carbon dioxide concentration is too high and affects the normal development of the lettuce sample, the circulating fan located inside the connecting pipe 351 is started to introduce the external air into the connecting pipe 351, and then flow into the purification cylinder 3 along the connecting pipe 351, and is located in the ventilation area 35 surrounded by the inner ring 32; Then, the air pressure inside the ventilation area 35 increases, so the air penetrates from the inside to the outside and passes through the purification area 34 in the gap between the inner ring 32 and the outer ring 33. Because the inner ring 32 and the outer ring 33 are both filter mesh structures, the impurities and dust in the air are filtered out during the process of passing through the inner ring 32, the outer ring 33 and the purification area 34. At the same time, the purification filler filled in the purification area 34 is selected from the existing antibacterial particles, such as nano silver antibacterial particles or antibacterial plastic particles. Therefore, when the air passes through the gaps in the purification filler, pollutants such as bacteria contained in the air are retained in the purification filler and are purified; the air that penetrates through the outer ring 33 then flows downward into the culture area 11, accelerating the air flow inside the culture area 11, and prompting the air with a high carbon dioxide content in the culture area 11 to flow out from the bottom air outlet 14, thereby achieving air purification inside the culture area 11, improving the normal growth environment of the lettuce samples, and ensuring the smooth progress of the genetic engineering research on lettuce.

[0023] Embodiment 2: On the basis of the first embodiment, a disinfection channel 331 is provided at the top of the outer ring 33, and the disinfection channel 331 extends upward to the top of the ventilation area 35, and a disinfection lamp 332 is provided on the lower surface of the top of the ventilation area 35 in the area surrounded by the disinfection channel 331. The disinfection lamp 332 here can be selected from existing ultraviolet sterilization lamps for disinfection; The inner ring 32 is rotatably connected to the closing plate 31, and a rotating device 321 is provided on the closing plate 31 at a position corresponding to the inner ring 32, so as to drive the inner ring 32 to rotate relative to the outer ring 33. Here, a micro motor can be selected as the rotating device 321. There are many possible technical solutions for how to drive the inner ring 32 to rotate. This embodiment provides a possible technical solution. Specifically, a ring gear is provided on the outer cylindrical surface of the end of the inner ring 32 embedded in the closing plate 31, and meshed with a driving gear on one side. The driving gear is connected to the rotating device 321. When started, the rotating device 321 drives the driving gear to rotate, thereby driving the meshing ring gear to drive the inner ring 32 to rotate slowly.

[0024] Specific workflow: Based on the specific workflow in Example 1, during the continuous operation of the purification cartridge 3, dust impurities and moisture in the air may adhere to the surface of the purification filler and accumulate in the gaps between the purification fillers, causing the purification particles to agglomerate into blocks; for the purification particles that tend to agglomerate with each other, more impurities will wrap the surface of the purification filler particles and prevent the normal performance of their bactericidal effect, causing bacteria in the impurities in the gaps to obtain breeding areas and environments, which is easy to pollute the air that penetrates through, and may further cause the purification cartridge 3 to reduce the air purification effect; Therefore, a disinfection channel 331 can be set on the top of the outer ring 33. The disinfection channel 331 is conical, and a disinfection lamp 332 is set on the top; the activated disinfection lamp 332 acts on the purification filler at the corresponding position to improve the sterilization effect on the purification filler itself; and relative to the fixedly installed outer ring 33, the inner ring 32 is rotatably connected through the rotating device 321. After the rotating device 321 is started, it drives the closing plates 31 on the opposite sides of the inner ring 32 to rotate slowly, and drives the contacted purification filler to rotate along the annular purification area 34, so that the purification fillers in various parts of the purification area 34 are fully stirred, and pass through the corresponding parts of the disinfection channel 331 in turn, and are sterilized and purified by the disinfection lamp 332; Furthermore, a heater may be provided on the inner wall of the disinfection channel 331, and a toggle block 322 may be evenly provided on the outer surface of the inner ring 32, and the end of the toggle block 322 may be bent; in this way, the end of the toggle block 322 is embedded in the purification filler, so that the friction between the inner ring 32 and the purification filler is enhanced, so that more purification fillers can be driven during the rotation of the inner ring 32, so that the purification filler flows more smoothly, and the disinfection lamp 332 can fully act on more purification fillers; Because of the relative rotation between the inner ring 32 and the outer ring 33, and the impact of the surface toggle block 322 on the purification filler during the rotation of the inner ring 32, the part of the purification filler that tends to form agglomerates is broken under the friction impact, exposing the internally wrapped part; then, when passing through the corresponding part of the disinfection channel 331, the broken aggregated purification filler is disinfected and heated by the heater at the same time, so that the water vapor contained in the adhered impurities is separated, the adhesion of the adhered impurities is reduced, and it is accelerated to separate from the surface of the purification filler, so that the purification filler that tends to form agglomerates is further dispersed from each other and fully purified by the disinfection lamp 332; through the rotation of the inner ring 32, the purification filler becomes more dispersed and loose, and the impurities and bacteria that may remain in the gap area are fully disinfected, the air permeability is guaranteed, and the passing air is fully disinfected, the continuous working time of the purification cylinder 3 is increased, the frequency of manual replacement of the purification cylinder 3 is reduced, and the safety of the lettuce sample in the culture dish 2 is also guaranteed.

[0025] Embodiment three: On the basis of the second embodiment, a dust collecting cylinder 36 is arranged on the back side of the closing plate 31 away from the ventilation port 121, and the dust collecting cylinder 36 is communicated with the interior of the ventilation area 35 through a connecting hole 361. An air duct 362 is arranged at the end of the dust collecting cylinder 36, and the air duct 362 connects the ventilation area 35 and the internal area of ​​the dust collecting cylinder 36, and an intercepting net 363 is arranged on the inner wall of the dust collecting cylinder 36 between the air duct 362 and the connecting hole 361.

[0026] Specific workflow: Based on the specific workflow in the second embodiment, the air flowing into the ventilation area 35 first needs to pass through the inner ring 32 before entering the purification area 34. Therefore, the inner ring 32 of the filter structure first performs a preliminary filtration on the air to remove the larger impurities and dust therein, and adheres to the surface of the inner ring 32 on the side close to the ventilation area 35. In order to prevent the accumulation of a large amount of dust and impurities from affecting the passability of the inner ring 32, a dust collecting cylinder 36 is provided on the back of the closing plate 31 on one side, so that after the air flows into the ventilation area 35 through the connecting pipe 351, a part of the air will flow into the dust collecting cylinder 36 through the connecting hole 361 horizontally; Because the opening of the connecting hole 361 is directly facing the surface of the inner ring 32, the tangential airflow formed by the inflowing air scours the surface of the inner ring 32 and brings the adhered dust into the dust collecting barrel 36, and after passing through the intercepting net 363 to remove dust and debris, it is replenished to the inside of the purification area 34 through the air guide pipe 362; in this way, on the one hand, the dust on the surface of the inner ring 32 is enriched in the dust collecting barrel 36, and the continuous accumulation of dust and impurities on the inner ring 32 is avoided to affect the passability of the inner ring 32, and the dust collecting barrel 36 located on the back of the closing plate 31 is located on the outside of the purification barrel 3, which can be easily dismantled and cleaned, and the operation is simple; on the other hand, the air that flows horizontally into the purification area 34 forms a horizontal airflow to impact the internal purification filler, prompting the purification filler to flow faster, and the flow path of the air that flows horizontally is longer, and the purification effect is more sufficient; Furthermore, when it is necessary to clean the inner ring 32 more thoroughly, the circulating fan inside the connecting channel can be turned off, and the micro air pump arranged inside the air duct 362 can be started, so that the airflow inside the ventilation area 35 flows horizontally into the dust collecting tube 36 through the connecting hole 361, and then flows into the purification area 34 through the air duct 362; because of the negative pressure formed by the exhaust inside the ventilation area 35, the air flowing into the purification area 34 is prompted to reversely pass through the inner ring 32 and flow into the ventilation area 35, forming an air circulation; the reverse flushing accelerates the dust and impurities adhering to the surface of the inner ring 32 to fall off, and are fully enriched in the dust collecting tube 36, thereby improving the cleaning efficiency of the inner ring 32 and ensuring the normal operation of the purification tube 3.

[0027] Embodiment 4: On the basis of the third embodiment, a squeeze ring 37 is provided on one side of the purification zone 34, and the squeeze ring 37 is slidably connected to the inner wall of the purification zone 34; a pushing device 371 is provided on the side wall of the ventilation zone 35, and the pushing device 371 here can be an existing electric telescopic device; the output end of the pushing device 371 slides through the corresponding closing plate 31 and is connected to the squeeze ring 37; A combing rod 372 is arranged in a circular distribution on the surface of the extrusion ring 37, and the combing rod 372 extends horizontally inside the purification area 34, and a combing block 373 is arranged on the combing rod 372 in the gap between the toggle blocks 322; the combing rod 372 on the extrusion ring 37 is a rotation connection, and a torsion spring is arranged at the rotation connection; a vibrator is arranged inside the extrusion ring 37, and the vibrator here can be a miniature vibration motor, which is controlled by an external controller; a limiting cylinder is arranged at the position corresponding to the combing rod 372 on the other side of the closing plate 31 away from the extrusion ring 37, and the end of the combing rod 372 is slidably embedded in the limiting cylinder, so that the end of the combing rod 372 on the side away from the extrusion ring 37 is supported and limited.

[0028] Specific workflow: Based on the specific workflow in Example 3, when the circulating fan is started and the air passes through the purification area 34, the pushing device 371 is started to slide horizontally and squeeze the purification filler in the purification area 34, so that the gap between the purification fillers is reduced, and the air in the gaps is compressed and flows out faster. The friction between the air flow and the surface of the purification filler accelerates the impurities adhering to the surface of the purification filler to fall off; At the same time, the sliding of the extrusion ring 37 drives the laterally extending combing rod 372 to move, and the moving distance is smaller than the gap between the toggle blocks 322, thereby avoiding collision between the extrusion ring 37 and the toggle blocks 322; the combing rod 372 drives the combing block 373 to impact the purification fillers that are squeezed against each other as the extrusion ring 37 slides laterally, so that the stirring effect on the purification fillers is aggravated, and then the purification fillers that have a tendency to agglomerate are dissociated and dispersed under the extrusion impact, and the loose purification fillers are in more sufficient contact with the passing air, and the air is purified more thoroughly; and because the extrusion ring 37 is in contact with the surface of the inner ring 32 and a vibrator is arranged inside, it vibrates continuously during the movement and transmits the vibration to the inner ring 32, causing the dust and debris adhering to the surface of the inner ring 32 close to the ventilation area 35 to fall off, and then are introduced into the dust collecting barrel 36 by the lateral airflow for collection; After sliding horizontally to the set displacement, the extrusion ring 37 is controlled to reset, and the extrusion ring 37 drives the combing rod 372 and the combing block 373 to reset, stirring the purification filler while accelerating the flow exchange of the purification filler in the horizontal direction, so that the purification filler is more fully disinfected when passing through the disinfection channel 331; Furthermore, when the inner ring 32 rotates, a gap is maintained between the toggle block 322 on the surface of the inner ring 32 and the combing rod 372, so that the rotation of the inner ring 32 will not be hindered by the combing rod 372, and the toggle block 322 and the combing block 373 are staggered with each other. During the rotation of the inner ring 32, the toggle block 322 and the combing block 373 rotate staggered with each other to fully stir the purification fillers in different areas of the purification area 34, and the purification fillers with larger agglomerates are broken under the extrusion impact of the staggered toggle block 322 and the combing block 373, making the purification fillers more loose and dispersed; when the combing rod 372 moves laterally with the extrusion ring 37, the toggle block 322 It overlaps with the combing block 373 and collides with it. Since the inner ring 32 rotates slowly, the impact of the collision is small, but it will make the combing block 373 tend to rotate, and drive the connected combing rod 372 to rotate relative to the extrusion ring 37, and then it will reset under the action of the connected torsion spring; the rotating combing block 373 pushes the purification filler to move vertically, causing the purification filler near the inner ring 32 inside the evolution zone to flow toward the direction close to the outer ring 33, so that the fully turned over purification filler passes through the disinfection channel 331 with the inner ring 32, so that the purification filler is more fully affected by the disinfection lamp 332, further improving the purification effect on the passing air.

[0029] Embodiment five: On the basis of the fourth embodiment, the end of the air guide tube 362 is introduced into the ventilation area 35 and is located in the gap between the closing plate 31 and the extrusion ring 37; the combing rod 372 is a tubular structure, and the end of the combing tube is connected to the gap area between the closing plate 31 and the extrusion ring 37; the end of the combing block 373 is evenly provided with impact holes 374, and the impact holes 374 are connected to the inside of the combing rod 372.

[0030] Specific workflow: Based on the specific workflow in the fourth embodiment, the air guide 362 inputs the purified air in the dust collecting tube 36 into the gap between the extrusion ring 37 and the closing plate 31, and then the airflow can directly flow into the connected combing rod 372, and then flow out from the impact hole 374 on the combing block 373. In this way, when the combing block 373 stirs the purification filler, the air can directly flow out from the impact hole 374 at the end of the combing block 373 and impact the contacted purification filler, thereby improving the impact combing effect on the purification filler and making the air fully contact with the purification filler and be disinfected; When the extrusion ring 37 moves, the gap area increases and accumulates a large amount of air, which increases the air pressure in the gap area and pushes the extrusion ring 37 to slide normally; when the extrusion ring 37 is reset, the pressurized air is accelerated to flow into the connected combing rod 372, and flows into the extruded purification filler from the impact hole 374, so that the purification filler is impacted by the airflow from the inside to the outside and the separation is accelerated; the above process is repeated in this way, so that the purification filler remains loose during the repeated extrusion and dispersion process, reducing the tendency of clumping.

[0031] Embodiment six: On the basis of Example 5, compressed balls 4 are mixed in the gaps between the purification fillers, the interior of the compressed balls 4 is hollow and made of elastic material; through holes are evenly arranged on the surface of the compressed balls 4, and the interior of the compressed balls 4 is filled with dry particles, which can be silica gel dry particles. The volume of the dry particles occupies one third to one half of the internal area of ​​the compressed balls 4, and the particle size of the dry particles is larger than the aperture of the through holes.

[0032] Specific working process: On the basis of the specific working process in the fifth embodiment, the surface of the compression ball 4 is made of a hydrophilic material, so that the water vapor in the air is easy to approach and contact the surface of the compression ball 4. As the inner ring 32 rotates, the purification filler is driven to flow. The compression ball 4 is collided with each other and compressed during this process, which accelerates the exchange of internal air with the outside world, and also promotes water vapor to pass through the through hole into the compression ball 4 and be absorbed by the dry particles inside. Because the dry particles inside the compression ball 4 are full, during the rotation of the inner ring 32, the compression ball 4 rolls and impacts the surrounding purification filler, and the dry particles in the hollow part of the compression ball 4 impact the compression ball 4 from the inside under the action of inertia, which intensifies the vibration of the compression ball 4 and further stirs the surrounding purification filler, thereby inhibiting the occurrence of the tendency of the purification filler to form agglomerates. Furthermore, when the purification fillers tend to agglomerate with each other, because the surface of the compressed ball 4 is smooth, moisture and impurities are easily adhered to the surface of the compressed ball 4, so it is easy to form agglomerated agglomerated fillers with the compressed ball 4 as the core; when the extrusion plate moves laterally to extrude the accumulated purification fillers, because the compressed ball 4 is compressed and deformed, more margin is provided for the purification fillers to be compressed, and the surface of the compressed ball 4 is deformed and dry air is released through the surface through holes, so that the purification material wrapped on the outside of the compressed ball 4 is impacted and falls off, further inhibiting the development of the agglomeration trend in the purification fillers, ensuring the smooth progress of air purification, thereby ensuring the maintenance of the internal environment of the culture area 11; Furthermore, there are many possible schemes for setting the size and shape of the compression ball 4. For example, the compression ball 4 may be evenly provided with conical protrusions to form a sea urchin-like structure, so that when the compression ball 4 flows and impacts between the purification fillers, the purification fillers are further agitated, accelerating the trend of their mutual flow and uniform dispersion within the purification area 34; The diameter of the compression ball 4 can also be set to be larger than the distance between adjacent toggle blocks 322, or larger than the distance between the combing rod 372 and the toggle block 322. By setting the volume of the compression ball 4, the toggle block 322 can drive the compression ball 4 to rotate as the inner ring 32 rotates, thereby strengthening its rolling tendency and preventing it from passing through the gap between the toggle blocks 322; as the combing rod 372 and the end of the toggle block 322 approach and intersect, the compression ball 4 can also be driven to move to the gap between the combing rod 372 and the end of the toggle block 322 to compress it, thereby strengthening the air exchange between the inner and outer areas of the compression ball 4.

[0033] Embodiment seven: Based on the sixth embodiment, as shown in the attached Figure 8 As shown, a method for genetic engineering genetic research and cultivation of lettuce, the cultivation method uses the above-mentioned cultivation equipment, and the specific steps of the cultivation method are: S1: Select lettuce seeds for sterile cleaning, use filter paper to absorb moisture from the lettuce seeds, and inoculate them into a special culture dish 2 for germination, and place the special culture dish 2 for germination in the culture area 11 inside the incubator 1 for culturing; S2: Through the display interface and control buttons of the LCD screen outside the box 12, the operator can adjust the temperature and humidity module and the ventilation module to adjust the temperature and humidity of the internal environment of the culture area 11 and the concentration of carbon dioxide in the air, so that the lettuce seed sample in the culture dish 2 can germinate and grow normally; S3: taking out the germinated lettuce seeds, cutting off part of the cotyledons as explants, and inoculating them with the leaf surface facing downward into the culture medium prepared in advance in the culture dish 2 for pre-culture; using the infection solution to infect the explants, and then preparing the callus induction medium to perform callus induction on the explant samples to induce the formation of explant callus tissue; S4: Callus screening is performed on the formed explants and callus tissues through callus screening medium, so that qualified samples are screened out and cultured into lettuce seedlings, and observations and records are made after development, and the recorded data are compared and analyzed.

[0034] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A lettuce genetic engineering genetic research and cultivation device, comprising a culture box (1) and a culture dish (2), wherein the culture dish (2) is placed in a culture area (11) inside the culture box (1), and the culture box (1) is used to ensure the normal growth of the lettuce sample inside the culture dish (2); the culture box (1) comprises a box body (12), a temperature and humidity module, a ventilation module and a disinfection module, and the ventilation module is used to realize air exchange between the culture area (11) and the external environment; Features: The ventilation module comprises a ventilation port (121) arranged at the top of the box (12); a ventilation cavity (13) is arranged at the top of the box (12) located above the culture area (11); a purification cylinder (3) is arranged inside the ventilation cavity (13); closing plates (31) are arranged at both ends of the purification cylinder (3); an inner ring (32) and an outer ring (33) are arranged between the closing plates (31) on both sides; the inner ring (32) is located in the middle of the area surrounded by the outer ring (33); and both the inner ring (32) and the outer ring (33) are mesh structures; The gap area between the inner ring (32) and the outer ring (33) is a purification area (34), and the purification area (34) is filled with purification fillers. The area surrounded by the inner ring (32) is a ventilation area (35), and the ventilation area (35) is connected to the ventilation port (121) through a connecting pipe (351); The bottom of the purification zone (34) is in communication with the incubator (1), and an air outlet (14) is provided at the bottom of the incubator (1), and the air outlet (14) is also in communication with the interior of the incubation zone (11).

2. The lettuce genetic engineering genetic research and cultivation equipment according to claim 1, characterized in that: A disinfection channel (331) is provided at the top of the outer ring (33), the disinfection channel (331) extends upward to the top of the ventilation area (35), and a disinfection lamp (332) is provided on the lower surface of the top of the ventilation area (35) in the area surrounded by the disinfection channel (331); The inner ring (32) and the closing plate (31) are rotatably connected, and a rotating device (321) is provided at a position of the closing plate (31) corresponding to the inner ring (32) for driving the inner ring (32) to rotate relative to the outer ring (33); The outer surface of the inner ring (32) is evenly provided with toggle blocks (322), and the ends of the toggle blocks (322) are bent.

3. The lettuce genetic engineering genetic research and cultivation equipment according to claim 2, characterized in that: A dust collecting cylinder (36) is arranged on the back side of the closing plate (31) away from the ventilation opening (121); the dust collecting cylinder (36) is communicated with the interior of the ventilation area (35) through a connecting hole (361); an air guide pipe (362) is arranged at the end of the dust collecting cylinder (36); the air guide pipe (362) communicates the ventilation area (35) and the interior area of ​​the dust collecting cylinder (36); and an interception net (363) is arranged on the inner wall of the dust collecting cylinder (36) between the air guide pipe (362) and the connecting hole (361).

4. The lettuce genetic engineering genetic research and cultivation equipment according to claim 3, characterized in that: A squeezing ring (37) is provided on one side of the purification zone (34), and the squeezing ring (37) is slidably connected to the inner wall of the purification zone (34); a pushing device (371) is provided on the side wall of the ventilation zone (35), and the output end of the pushing device (371) slides through the corresponding closing plate (31) and is connected to the squeezing ring (37).

5. The lettuce genetic engineering genetic research and cultivation equipment according to claim 4, characterized in that: Combing rods (372) are arranged in an annular pattern on the surface of the extrusion ring (37), the combing rods (372) extend transversely inside the purification zone (34), and combing blocks (373) are arranged in the areas of the combing rods (372) located in the gaps between the toggle blocks (322).

6. The lettuce genetic engineering genetic research and cultivation equipment according to claim 5, characterized in that: The combing rod (372) on the extrusion ring (37) is rotatably connected, and a torsion spring is arranged at the rotatably connected position; a vibrator is arranged inside the extrusion ring (37).

7. The lettuce genetic engineering genetic research and cultivation equipment according to claim 5, characterized in that: The end of the air guide tube (362) is introduced into the interior of the ventilation area (35) and is located in the gap between the closing plate (31) and the extrusion ring (37); the combing rod (372) is a tubular structure, and the end of the combing tube is communicated with the gap area between the closing plate (31) and the extrusion ring (37); impact holes (374) are evenly arranged on the end of the combing block (373), and the impact holes (374) are communicated with the interior of the combing rod (372).

8. The lettuce genetic engineering genetic research and cultivation equipment according to claim 1, characterized in that: Compressed balls (4) are mixed in the gaps between the purification fillers; the compressed balls (4) are hollow inside and are made of elastic material.

9. The lettuce genetic engineering genetic research and cultivation equipment according to claim 8, characterized in that: Through holes are evenly arranged on the surface of the compression ball (4), and dry particles are filled inside the compression ball (4), wherein the particle size of the dry particles is larger than the aperture of the through holes.

10. A method for cultivating lettuce for genetic engineering research, the method using the cultivation equipment described in any one of claims 1 to 9, characterized in that: The specific steps of the culture method are: S1: selecting lettuce seeds, washing them aseptically, drying the lettuce seeds with filter paper, and inoculating them into a special germination culture dish (2), and placing the special germination culture dish (2) into the internal culture area (11) of the culture box (1) for culture; S2: Through the display interface and control buttons of the liquid crystal screen outside the box (12), the operator can adjust the temperature and humidity module and the ventilation module to adjust the temperature and humidity of the internal environment of the culture area (11) and the concentration of carbon dioxide in the air, so that the lettuce seed sample in the culture dish (2) can germinate and grow normally; S3: taking out the germinated lettuce seeds, cutting off part of the cotyledons as explants, and inoculating them with the leaf surface facing downward into the culture medium prepared in advance in the culture dish (2) for pre-culture; using the infection solution to infect the explants, and then preparing a callus induction medium to perform callus induction on the explant samples to induce the formation of explant callus tissue; S4: Callus screening is performed on the formed explants and callus tissues through callus screening medium, so that qualified samples are screened out and cultured into lettuce seedlings, and observations and records are made after development, and the recorded data are compared and analyzed.

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