Composition and related application thereof in whitening

Abstract

The invention discloses a composition and related application thereof in whitening, and relates to the field of cosmetics, the composition comprises the following components in parts by weight: 75-125 parts of nicotinamide, 25-75 parts of magnolia sieboldii extract and 1-10 parts of hydrolyzed conchiolin protein, the three components are mixed according to a specific proportion, and can achieve a synergistic effect, so that the whitening effect is improved, and the whitening effect is improved. The whitening composition has the advantages that melanin synthesis is obviously inhibited, melanin transfer and metabolism are promoted, and the content of melanin is reduced in multiple directions and multiple levels, so that the whitening effect is effectively exerted, and a new way is provided for efficient, safe and low-irritation whitening.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a composition and related applications thereof in whitening. Background Art

[0002] At present, there are many whitening skin care products on the market, but the whitening mechanism of most products is relatively simple, basically staying at the simple superposition of ingredients with the same mechanism, without a scientific and rigorous evaluation system to optimize the whitening effect under the same mechanism, and lacking obvious effects. Therefore, it is necessary to develop an efficient, safe and low-irritation whitening formula.

[0003] In view of this, the present invention is proposed. Summary of the invention

[0004] The purpose of the present invention is to provide a composition and its related application in whitening.

[0005] The present invention is achieved in that:

[0006] In a first aspect, an embodiment of the present invention provides a composition comprising, by weight, 75 to 125 parts of niacinamide, 25 to 75 parts of Magnolia sieboldii extract, and 1 to 10 parts of hydrolyzed conchiolin.

[0007] In a second aspect, an embodiment of the present invention provides a skin care product, the active ingredient of which includes the composition described in the above embodiment.

[0008] In a third aspect, an embodiment of the present invention provides a cosmetic, the active ingredient of which includes the composition described in the above embodiment.

[0009] In a fourth aspect, embodiments of the present invention provide use of the composition described in the preceding embodiments in the preparation of whitening products.

[0010] In a fifth aspect, embodiments of the present invention provide the use of the composition as described in the preceding embodiments in any one of inhibiting melanin synthesis, promoting melanin transport, promoting melanin metabolism and reducing melanin content for whitening and non-disease treatment purposes.

[0011] The present invention has the following beneficial effects:

[0012] By mixing niacinamide, magnolia sieboldii extract and hydrolyzed conchiolin in a specific proportion, the three components work synergistically to significantly inhibit melanin synthesis, promote melanin transport and metabolism, and reduce the melanin content in multiple directions and levels, thereby effectively exerting the whitening effect and providing a new way for efficient, safe and low-irritation whitening. DETAILED DESCRIPTION

[0013] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0014] In response to the problems existing in the prior art, the inventors of the present application have found through extensive research that by mixing niacinamide, magnolia sieboldii extract, and hydrolyzed conchiolin in a specific proportion and using the three together, it is possible to inhibit melanin synthesis, promote melanin transport and metabolism, and reduce the melanin content in multiple directions and at multiple levels, thereby having a significant synergistic effect in whitening and producing an effect of 1+1+1>3.

[0015] On the one hand, an embodiment of the present invention provides a composition, which includes, by weight: 75 to 125 parts of niacinamide, 25 to 75 parts of Magnolia sieboldii extract and 1 to 10 parts of hydrolyzed conchiolin.

[0016] In some embodiments, the weight percentage of niacinamide can be any one of 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125 parts or a range between any two of them.

[0017] Magnolia sieboldii extract is extracted from the flowers and branches of Magnolia sieboldii. In some embodiments, the Magnolia sieboldii extract can be obtained by extracting using an existing disclosed process or using a commercially available Magnolia sieboldii extract, and the active ingredient in the Magnolia sieboldii extract includes magnolol, and the content of magnolol in the Magnolia sieboldii extract is greater than 0.009% (weight percentage).

[0018] In some embodiments, the extraction process of Magnolia sieboldii extract can be a mixture of water and ethanol mixed in a certain proportion, or 1,3-butanediol can be used as the extractant, and the ratio of the extractant to the Magnolia sieboldii plant can be 10:1. After heating extraction, the extract is filtered and concentrated, and finally 30-60% 1,3-butanediol is added to the concentrate to obtain the Magnolia sieboldii extract.

[0019] In some embodiments, the weight portion of the Magnolia sieboldii extract can be any one of 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75 parts or a range between any two of them.

[0020] Hydrolyzed conchiolin is a compound extracted and separated from shells. The hydrolyzed conchiolin provided in the embodiment of the present invention can be extracted and obtained by using the existing disclosed process or commercially available hydrolyzed conchiolin. The hydrolyzed conchiolin contains 85wt% to 100wt% of protein, specifically 85wt%, 86wt%, 88wt%, 90wt%, 92wt%, 94wt%, 96wt%, 98wt%, 100wt% of any one or any range of protein between the two. In some embodiments, the preparation method of hydrolyzed conchiolin may include but is not limited to grinding the shells of the cuming oyster into powder in a grinder, adding an organic acid at 60°C to fully react, filtering and decalcifying to obtain the conchiolin, then adding protease at 50°C, filtering after enzymolysis for 5 hours, and taking the supernatant and freeze-drying it.

[0021] In some embodiments, the hydrolyzed conchiolin protein can be any one of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 parts or a range between any two of them.

[0022] In some embodiments, the composition comprises, by weight: 90 to 110 parts of niacinamide, 40 to 60 parts of Magnolia sieboldii extract, and 1 to 5 parts of hydrolyzed conchiolin.

[0023] On the other hand, an embodiment of the present invention provides a skin care product, the active ingredient of which includes the composition described in any of the above embodiments.

[0024] In some embodiments, the skin care product comprises any one of: lotion, essence, face cream, eye cream, face mask, hydrating spray, body lotion and hand cream.

[0025] In some embodiments, the serum comprises a whitening serum.

[0026] In some embodiments, the whitening essence comprises, by weight percentage: 0.1% to 6% of the composition, 0.4% to 2% of an emulsifier, 2% to 10% of an emollient, 1% to 10% of a moisturizer, 0.05% to 3% of a thickener, 0.1% to 2% of a preservative and the remainder of water.

[0027] In some embodiments, the percentage of the composition in the whitening essence can be any one of 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, or any two of the range. The percentage of the emulsifier in the whitening essence can be any one of 0.4%, 0.5%, 1%, 1.5%, 2%, or any two of the range. The percentage of the emollient in the whitening essence can be any one of 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, or any two of the range. The percentage of the moisturizer in the whitening essence can be any one of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, or any two of the range. The percentage of thickener in the whitening essence can be any one of 0.05%, 0.1%, 0.3%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, or any range between two of them. The percentage of preservative in the whitening essence can be any one of 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, or any range between two of them.

[0028] In some embodiments, the emulsifier may exemplarily but not limitatively include at least one of sorbitan stearate and inulin lauryl carbamate; the emollient may exemplarily but not limitatively include at least one of dimethicone, hydrogenated lecithin, caprylic / capric triglyceride, pentaerythritol tetra(ethylhexanoate), and octyldodecanol; the humectant may exemplarily but not limitatively include at least one of polyols (such as glycerol and 1,3-propylene glycol), ethylhexylglycerin, sodium hyaluronate, and panthenol; the thickener may exemplarily but not limitatively include at least one of ammonium acryloyldimethyltaurate / VP copolymer, sodium polyacryloyldimethyltaurate, and carbomer; the preservative may exemplarily but not limitatively include at least one of phenoxyethanol, p-hydroxyacetophenone, caprylhydroxamic acid, methylparaben, sodium benzoate, pentylene glycol, and 1,2-hexanediol.

[0029] The present invention has no special restrictions on the preparation process of the whitening essence, and the preparation process can be carried out using the existing disclosed process. Specifically, the preparation process may include the following steps:

[0030] Add emulsifier, moisturizer, thickener and preservative into the water phase pot according to the proportion, heat to 75-80°C, stir until completely dissolved to obtain the water phase; pump the water phase into the emulsifying pot, and homogenize at a speed of 3000 rpm; add the emollient into the emulsifying pot while homogenizing, and homogenize evenly; cool to below 45°C, add the whitening composition, stir evenly, and obtain the whitening essence.

[0031] On the other hand, an embodiment of the present invention provides a cosmetic, the active ingredient of which includes the composition described in any of the above embodiments.

[0032] The present invention has no special restrictions on the base part of the skin care products and cosmetics, and the base of the existing public or commercially available skin care products and cosmetics can be used. The amount of the composition added to the cosmetics and skin care products can be selectively added based on actual needs, for example, the amount of the composition added can be 0.1% to 6% by weight of the cosmetics and skin care product base.

[0033] On the other hand, an embodiment of the present invention provides use of the composition as described in any of the above embodiments in preparing a whitening product.

[0034] In some embodiments, the whitening includes any one or more effects of inhibiting melanin synthesis, promoting melanin transport, promoting melanin metabolism and reducing the content of melanin.

[0035] In addition, an embodiment of the present invention provides the use of the composition as described in any of the foregoing embodiments in any of the following applications: whitening, inhibiting melanin synthesis for non-disease treatment purposes, promoting melanin transport, promoting melanin metabolism, and reducing melanin content.

[0036] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0037] Examples 1 to 4 and Comparative Examples 1 to 4

[0038] Examples 1 to 4 and Comparative Examples 1 to 4 respectively provide a composition, the difference being that the components and the ratios of the components are different, as shown in Table 1. The values ​​corresponding to the components in the table are the weight ratios of the components in the composition based on hydrolyzed conchiolin protein (except kojic acid). The Magnolia sieboldii extract in the examples and comparative examples is the MAGNOL1A EXTRACT product produced by Bioland, with the production batch number being MSEEC-010163; the hydrolyzed conchiolin protein is produced by Deqing Oushiman Biotechnology Co., Ltd., with the production batch number being 240619; Note: The preparation process of hydrolyzed conchiolin protein (INCI: HYDROLYZEDCONCHIOLIN PROTEIN) refers to Example 2 in Patent No.: CN111850073A (the invention name is Pearl Shell Whitening Factor, Preparation Method and Use thereof).

[0039] Table 1 Composition

[0040]

[0041]

[0042] Note: Comparative Example 4 is a blank control, which is PBS phosphate buffer (pH 7.2-7.4).

[0043] After preparing the compositions according to the ratios in Table 1, the melanin content of B16 cells was detected by the following method, and 0.2 mg / mL kojic acid was used as the positive control.

[0044] B16 cells in the logarithmic growth phase grown in DMEM medium were cultured at 7.5×10 4 The number of cells / well was inoculated in a 6-well plate, 2 mL of culture medium was added to each well, and the plate was incubated in a carbon dioxide incubator at 37°C and 5% CO2 concentration. After 24 hours, 200 μl of sample (Table 1) was added to each well, and 3 replicates were set for each concentration, and the incubation continued. After 24 hours of culture, the cells were collected by trypsin digestion, the supernatant was discarded, and the cells were washed twice with PBS and the supernatant was discarded. 150 μl of 1N NaOH containing 10% DMSO was added to each tube to lyse the cells, and the plates were water bathed at 80°C for 30 minutes. 100 μL of cell lysate was aspirated and added to a 96-well plate, and the absorbance OD value was measured at 405 nm using an enzyme reader. The average value of the data from the three replicates was taken as the final result.

[0045] Melanin inhibition rate calculation:

[0046] Melanin inhibition rate = (OD cell control group OD experimental group) / OD cell control group × 100%.

[0047] From the comparison of Examples 1 to 4, it can be seen that the raw material ratios of different compositions can affect the whitening effect of the whitening composition. Under the premise that the quality and the ratio of niacinamide, magnolia sieboldii extract and hydrolyzed conchiolin remain unchanged, the ratio of the three is within the range of 125:25:1 and 75:75:1, which all have synergistic whitening effects among the three. Among them, the optimal ratio is 100:50:1.

[0048] By comparing Example 2 with Comparative Examples 1 to 4, it can be seen that the combination of niacinamide, Magnolia sieboldii extract and hydrolyzed conchiolin in the composition provided by the embodiment of the present invention has a significant synergistic effect in whitening, and its whitening effect is significantly higher than that of kojic acid (positive control).

[0049] Comparative Example 2 and Comparative Example 5 show that when niacinamide is replaced with another traditional whitening agent, tranexamic acid, although the ratio of niacinamide to magnolia sieboldii extract and hydrolyzed conchiolin is the same, it does not show a synergistic whitening effect.

[0050] Embodiments 5 to 8

[0051] Examples 5 to 8 respectively provide whitening essences, and the compositions in the whitening essences described in Examples 5 to 8 are the compositions provided in Examples 1 to 4, respectively, that is, the composition in the whitening essence described in Example 5 is the composition described in Example 1, the composition in the whitening essence described in Example 6 is the composition described in Example 2, the composition in the whitening essence described in Example 7 is the composition described in Example 3, and the composition in the whitening essence described in Example 8 is the composition described in Example 4.

[0052] In Examples 5 to 8, the components and weight percentages of the whitening essence are shown in Table 2.

[0053] Table 2 Whitening Essence

[0054]

[0055]

[0056] The preparation method of whitening essence is as follows:

[0057] (1) According to the ratio in Table 2, glycerin, 1,3-propylene glycol, sodium polyacryloyldimethyl taurate, p-hydroxyacetophenone, 1,2-hexanediol, panthenol, sodium hyaluronate, inulin lauryl carbamate and deionized water are added to a water phase pot, heated to 75°C, and stirred evenly to obtain a water phase; (2) The water phase is pumped into an emulsifying pot and homogenized at a speed of 3000 rpm; (3) Polydimethylsiloxane, pentaerythritol tetra(ethylhexanoate) ester and caprylic / capric triglyceride are added to the emulsifying pot while homogenizing, and homogenized at a speed of 3000 rpm; (4) The temperature is lowered to 45°C, and the composition is added and stirred evenly to obtain a whitening essence.

[0058] Comparative Examples 5 to 8

[0059] Comparative Examples 5 to 8 respectively provide whitening essences, the difference being that the compositions in the whitening essences are different. The compositions in the whitening essences in Comparative Examples 5 to 8 are the compositions provided in Comparative Examples 1 to 4, respectively. The components and weight percentages of the whitening essences are shown in Table 2.

[0060] The preparation method of the whitening essence provided in Comparative Examples 5 to 8 is the same as the preparation method of the whitening essence described in Examples 5 to 8.

[0061] Test Example 1

[0062] Human whitening test

[0063] The skin melanin content (MI) value is used to represent the melanin index. The larger the MI value, the higher the skin melanin content. The skin brightness L* value is used to represent the blackness and whiteness of the skin. The larger the value, the whiter the skin color.

[0064] Experimental method: The samples prepared in Examples 5 to 8 and Comparative Examples 5 to 8 were respectively investigated for population trials, and samples were distributed to 180 female volunteers aged 20 to 50 years old, who were healthy, had no allergies to cosmetics, no acute facial inflammation, did not use hormone drugs or immunosuppressants, and did not participate in other clinical trials. The volunteers were randomly divided into 9 groups, each group used the same formula product, one of which was a commercially available whitening product. After cleaning the facial skin every morning and evening, the volunteers applied 0.5 grams of whitening essence to the entire face by hand. No other cosmetics or drugs were applied to the face during the test period. The products were used continuously for 4 weeks, and the changes in skin melanin MI and L* before and after the subjects used the corresponding products were evaluated with a CK multifunctional skin tester to determine the whitening effect of the product.

[0065] The difference in melanin content △MI = MI value after product use × MI value before product use.

[0066] Brightness difference △L*=L* value after product use × L* value before product use.

[0067] Table 3 Changes in skin melanin value MI and brightness L*

[0068]

[0069] It can be seen from Table 3 that the composition provided by the embodiment of the present invention has good whitening and brightening effects and can effectively improve skin color.

[0070] By comparing Examples 5 to 8, it can be seen that different ratios of whitening compositions can affect the improvement effect on skin color (whitening and brightening), among which Example 6 is the best ratio, that is, the whitening essence prepared using the whitening composition described in Example 2 has better whitening and brightening effects.

[0071] By comparing Example 6 with Comparative Examples 5 to 8, it can be seen that the combination of niacinamide, Magnolia sieboldii extract and hydrolyzed conchiolin in the embodiment of the present invention has a significant synergistic effect in whitening and brightening.

[0072] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composition, characterized in that Calculated by weight, it comprises: 75-125 parts of niacinamide, 25-75 parts of magnolia sieboldii extract and 1-10 parts of hydrolyzed conchiolin.

2. The composition according to claim 1, characterized in that The composition comprises, by weight, 90 to 110 parts of niacinamide, 40 to 60 parts of magnolia sieboldii extract and 1 to 5 parts of hydrolyzed conchiolin.

3. A skin care product, characterized in that: The active ingredients thereof include: the composition according to claim 1 or 2.

4. The skin care product according to claim 3, characterized in that: The skin care products include: any one of lotion, essence, face cream, eye cream, facial mask, moisturizing spray, body lotion and hand cream.

5. The skin care product according to claim 4, characterized in that: The essence comprises a whitening essence.

6. The skin care product according to claim 5, characterized in that According to weight percentage, the whitening essence comprises: 0.1% to 6% of the composition, 0.5% to 3% of an emulsifier, 2% to 10% of an emollient, 1% to 10% of a moisturizer, 0.05% to 3% of a thickener, 0.1% to 1% of a preservative and the balance of water.

7. A cosmetic, characterized in that: The active ingredients thereof include: the composition according to claim 1 or 2.

8. Use of the composition as claimed in claim 1 or 2 in the preparation of whitening products.

9. The use according to claim 8, characterized in that: The whitening includes any one or more effects of inhibiting melanin synthesis, promoting melanin transport, promoting melanin metabolism and reducing melanin content.

10. Use of the composition according to claim 1 or 2 in any one of whitening, inhibiting melanin synthesis for non-disease treatment purposes, promoting melanin transport, promoting melanin metabolism and reducing melanin content.

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