A whitening and anti-aging composition and preparation method thereof

Abstract

The present invention provides a whitening and anti-aging composition and a preparation method thereof. The composition uses composite probiotic freeze-dried powder as a core component and is encapsulated using microencapsulation technology, wherein dopamine and hyaluronic acid-modified calcium alginate are used as an inner layer, and maltodextrin modified with syringic acid is used as an outer layer. This double-layer structure design can effectively protect the probiotics from being destroyed by gastric acid and bile salts, ensuring that the probiotics maintain a high survival rate and stability in the microcapsule. Fish collagen peptides, mannose and vitamin C are also specially added to the composition, which have the ability to reduce free radicals, help reduce cell damage, and delay skin aging. Combined with traditional Chinese medicine ingredients Panax notoginseng powder and edible spirulina powder, the composition can prevent dry and rough skin, and can also provide special skin care effects. These ingredients can promote the balance of intestinal flora, achieve a beauty effect of both internal and external cultivation, and enhance the efficacy of the composition in whitening skin, improving complexion and anti-aging.

Description

Technical Field

[0001] The invention belongs to the technical field of whitening and anti-aging health care products, and specifically relates to a whitening and anti-aging composition and a preparation method thereof. Background Art

[0002] Skin aging is a complex process caused by oxidation, which leads to damage to biomacromolecules, manifested as skin sagging, wrinkles, pigmentation, roughness, thinning, and weakened keratin barrier function. At the cellular level, aged skin shows a slowdown in epidermal cell renewal, reduced keratinocyte activity, and a decrease in the number of fibroblasts in the dermis, and the ability of these cells to synthesize collagen and elastin also decreases. Although the human body has its own antioxidant defense mechanisms, these mechanisms are not sufficient to completely protect or repair the damage caused by oxidative damage. Therefore, supplementing antioxidants helps maintain the balance of oxygen metabolism in the body. With the growing interest in skin whitening and maintaining youthful and vibrant skin, preventing skin aging has become the key to achieving whitening and maintaining youthful skin. In short, one of the effective ways to have fair and youthful skin is to prevent skin aging.

[0003] The market demand for anti-aging products is growing steadily, and probiotics, as a type of active microorganisms that are beneficial to the host, have been widely reported to have a variety of physiological effects. These effects include improving the intestinal environment, regulating the balance of the flora, enhancing immunity, and alleviating allergic symptoms. At present, the research on the anti-aging function of probiotics is gradually increasing, and the research focus is mainly on the regulation of intestinal flora, anti-oxidation, and immune regulation. However, there are relatively few studies on health care products that have both skin whitening and anti-aging effects. In addition, most of the strains used to regulate intestinal flora or anti-oxidation are single lactic acid bacteria. Due to the limited function of a single strain, it may not be possible to maximize the advantages of the strain for such a complex internal environment as the intestine. At present, there is no multi-strain compound method that has been widely used in the field of delaying aging and whitening skin.

[0004] Probiotics are valued for their many health benefits, including anti-aging and skin whitening. However, they are easily damaged or even lose their activity under extreme conditions. To address this problem, probiotic microencapsulation technology can protect probiotics to maintain high survival rates during production, storage, and passage through the gastrointestinal tract. Currently, a variety of probiotic microencapsulation technologies have been applied, showing good stability and sensory properties. Nevertheless, these technologies cannot guarantee that probiotics can maintain optimal activity in all environments, so further research is needed to optimize their application in health foods. In addition, some microencapsulation methods are costly, and in order to make probiotic microencapsulation products competitive, costs must be controlled within an acceptable range. Therefore, in the industrial production of probiotic microencapsulation products, in addition to paying attention to the survival rate of the product, cost-effectiveness and activity retention during the shelf life should also be emphasized.

[0005] Therefore, improving the number of viable probiotics and ensuring their survival under adverse conditions are technical problems that need to be solved urgently. In response to this problem, the present invention uses microcapsule technology to embed probiotics, aiming to improve the survival ability of probiotics in various adverse environments, and adding this microencapsulated probiotics to health products can not only effectively protect the activity of probiotics, resist the influence of the external environment on their survival, but also significantly reduce the destruction of gastric acid and digestive enzymes to the activity of probiotics. In this way, probiotics can maintain a high number and activity when reaching the human intestine, so as to better play the effect of whitening skin and anti-aging. Summary of the invention

[0006] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide a whitening and anti-aging composition and its preparation method and application. The composition is made into a microcapsule with a specific structure by compound probiotics, so that it can resist the damage of gastric acid and bile salts, safely reach the intestine and play its role. This innovative method significantly improves the efficacy of the composition in whitening skin, improving complexion and anti-aging.

[0007] The invention provides a whitening and anti-aging composition, which comprises, by weight: 3-10 parts of fish collagen peptides, 1-8 parts of compound probiotic freeze-dried powder, 5-15 parts of edible spirulina powder, 10-15 parts of notoginseng powder, 5-10 parts of vitamin C, 3-8 parts of mannose, and 10-20 parts of starch.

[0008] The composite probiotic freeze-dried powder is prepared by a preparation method comprising the following steps:

[0009] S1. Dissolve hyaluronic acid in deionized water, then add appropriate amounts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide, stir evenly, and prepare a hyaluronic acid mixed solution;

[0010] S2, mixing sodium alginate and soy protein in deionized water, and dispersing them uniformly by ultrasonic treatment, followed by sterilization, and then adding composite microbial powder and stirring them fully to prepare a uniform composite microbial powder suspension;

[0011] S3, synchronously dropping the hyaluronic acid mixed solution of step S1 and the composite microbial powder suspension of step S2 into the calcium salt solution, stirring for reaction for 1-3 hours, then dropping dopamine hydrochloride aqueous solution for reaction for 1-5 hours, washing, freeze-drying to obtain the microcapsule core material;

[0012] S4, mixing and dissolving sialic acid and maltodextrin in water to form a coating material solution of 2-10wt% sialic acid and 10-20wt% maltodextrin, and then using this solution to atomize and coat the microcapsule core material obtained in step S3, and finally freeze-drying to obtain a composite probiotic freeze-dried powder.

[0013] Preferably, the mass concentration of hyaluronic acid in the mixed solution of step S1 is 1-10%; wherein the mass contents of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide relative to hyaluronic acid are both 5-20%; the stirring mixing temperature in step S1 is 30-50° C., and the stirring time is 1-3 h.

[0014] Preferably, the concentration of sodium alginate in the suspension of step S2 is 3-8wt%; the concentration of soy protein is 1-3.5wt%; the ultrasonic conditions are: ultrasonic power is 300-400W, ultrasonic room temperature is 20-35°C, and ultrasonic time is 10-15min. The mass concentration of bacterial powder in the suspension of step S2 is 1-3.5%.

[0015] Preferably, in step S3, the mass ratio of the hyaluronic acid mixture, the composite microbial powder suspension, and the dopamine hydrochloride aqueous solution is 1-2:5-10:1-3, wherein the mass concentration of dopamine hydrochloride in the dopamine hydrochloride aqueous solution is 1-5%. The stirring reaction conditions are: the reaction temperature is 20-40°C, and the reaction time is 5-10h.

[0016] The calcium salt solution includes any one of calcium gluconate, calcium hydrogen phosphate or calcium chloride, and the mass concentration of the calcium salt is 1-5%. The freeze drying conditions of step S3 are: freeze drying at -60 ~ -70°C for 2-3h.

[0017] Preferably, in step S4, the conditions for atomization coating are: atomization pressure 0.1-0.5 MPa, air inlet temperature 50-60°C, air outlet temperature 25-40°C, coating chamber temperature 40-50°C; the flow rate of the coating material solution is set to 0.3 mL / min-1.0 mL / min.

[0018] Preferably, the composite microbial powder in step S2 is a mixture of Lactobacillus salivarius powder, Lactobacillus rhamnosus powder and Bifidobacterium longum powder in a mass ratio of 0.5-1:1-2:1-2; the effective viable count of the Lactobacillus salivarius powder, Lactobacillus rhamnosus powder and Bifidobacterium longum powder is 1×10 9 cfu / g—1×10 12 cfu / g.

[0019] Preferably, the whitening and anti-aging composition of the present invention may further include 10-30 parts of food acceptable auxiliary materials, wherein the auxiliary materials include one or more of dietary fiber, fruit and vegetable powder, edible flavors, flavoring agents, colorants and excipients.

[0020] The dietary fiber is at least one of fructooligosaccharides, xylooligosaccharides, galacto-oligosaccharides, isomaltooligosaccharides and oats.

[0021] The fruit and vegetable powder is one of orange powder, lemon powder, carrot powder, purple potato powder, yam powder, spinach powder or coconut powder, or a mixture of two or more thereof.

[0022] The edible flavor is one of orange flavor, orange flavor, lemon flavor, cherry flavor, menthol, apple flavor or coconut flavor, or a mixture of two or more thereof.

[0023] The flavoring agent is one or a mixture of two or more of sucralose, AK sugar, aspartame or mogroside.

[0024] The toner is one or a mixture of two or more of caramel color, gardenia yellow, curcumin or chlorophyll.

[0025] The excipient is one or a mixture of two or more of sodium carboxymethyl starch, xanthan gum or sodium carboxymethyl cellulose.

[0026] The whitening and anti-aging composition of the present invention is in the form of powder, tablet, granule or oral liquid.

[0027] Furthermore, the preparation method of the whitening and anti-aging composition of the present invention comprises the following steps:

[0028] 3-10 parts of fish collagen peptide, 1-8 parts of compound probiotic freeze-dried powder, 5-15 parts of edible spirulina powder, 10-15 parts of Panax notoginseng powder, 5-10 parts of vitamin C, 3-8 parts of mannose, and 10-20 parts of starch are mixed and processed to prepare a whitening and anti-aging composition;

[0029] Or 3-10 parts of fish collagen peptide, 1-8 parts of compound probiotic freeze-dried powder, 5-15 parts of edible spirulina powder, 10-15 parts of Panax notoginseng powder, 5-10 parts of vitamin C, 3-8 parts of mannose, and 10-20 parts of starch are mixed to obtain a premix, and then the premix is ​​mixed with auxiliary materials to prepare a whitening and anti-aging composition.

[0030] The invention inoculates the Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus salivarius into fermentation medium respectively to obtain fermentation liquid; then centrifuges the obtained fermentation liquid, collects the bacterial cells, freezes and dries them to obtain Lactobacillus rhamnosus powder, Bifidobacterium longum powder and Lactobacillus salivarius powder respectively.

[0031] The composite probiotics of the present invention are composed of Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus salivarius. The inventors have found that Lactobacillus rhamnosus has excellent free radical scavenging ability, can inhibit the activity of elastase, tyrosinase and hyaluronidase, so as to play the effects of anti-wrinkle, anti-aging, moisturizing and whitening. Although the antioxidant capacity of Lactobacillus salivarius itself is relatively weak, when it is mixed with Lactobacillus rhamnosus, the overall antioxidant performance can be significantly enhanced. Bifidobacterium longum and Lactobacillus salivarius are two probiotics that have a positive effect on intestinal health. Bifidobacterium longum can promote digestion, effectively regulate the balance of intestinal flora, and reduce the pH value of the intestinal environment by producing organic acids and antibacterial compounds, thereby inhibiting the growth of pathogenic bacteria; Lactobacillus salivarius has good antibacterial activity, immunomodulatory effect and the ability to regulate intestinal microbial flora. The present invention uses these three strains in combination, not only to play the effects of anti-aging and whitening, but also to have a positive impact on the composition of intestinal microbial flora, improve the metabolic characteristics of intestinal microbial flora, and then promote the formation of a healthy and balanced internal environment.

[0032] The present invention innovatively uses composite probiotics as the core material and encapsulates them through microencapsulation technology. In this process, soy protein, as part of the core material, can effectively protect the probiotics and reduce their losses during processing and storage, thereby maintaining the stability and activity of the probiotics to the greatest extent and effectively preventing the decline of the activity of the probiotics. In the structural design of the microcapsule, calcium alginate is used as the inner layer, and maltodextrin modified with syringic acid is used as the outer layer. Such a double-layer structure can protect the probiotics from damage by gastric acid and bile salts, ensuring that the probiotics maintain a high survival rate and stability in the microcapsule.

[0033] In the process of preparing the inner core of the microcapsule, sodium alginate first reacts with calcium ions to form calcium alginate, thereby achieving the initial encapsulation of the composite probiotics. Subsequently, hyaluronic acid is loaded onto the surface of calcium alginate, and then dopamine hydrochloride is added to react the amino group in dopamine with the carboxyl group in hyaluronic acid to form an amide bond, and dopamine is grafted onto hyaluronic acid to further encapsulate the probiotics. This step not only makes up for the possible incompleteness of calcium alginate encapsulation, but also makes the inner core surface denser, effectively preventing the leakage of probiotics, thereby increasing the encapsulation rate of the composite probiotics.

[0034] The present invention uses saccharin modified maltodextrin as the outer layer wrapping material, and utilizes the low solubility of saccharin in gastric juice to protect the probiotics from being eroded by gastric acid. Since saccharin has a unique chemical structure, it will not decompose in gastric juice, but can directly enter the intestine. In the intestine, saccharin can combine with minerals such as calcium ions and certain vitamins (such as vitamin B12). This characteristic enables saccharin to accurately transport probiotics to the intestine and release them, thereby exerting its probiotic effect.

[0035] Furthermore, the whitening and anti-aging composition of the present invention is specially added with fish collagen peptide, which not only has excellent free radical scavenging ability, but also can significantly improve the moisturizing ability of skin tissue cells, thereby maintaining the elasticity and toughness of the skin. Fish collagen peptide helps to delay the aging of the body, achieve the effects of moisturizing the skin, delaying aging, beautifying and reducing wrinkles, and shows excellent skin care effects.

[0036] The mannose used in the present invention has a certain reducing property and can remove free radicals in the body, thereby delaying the process of cell aging. More importantly, mannose is not digested and absorbed by the human gastrointestinal tract, but directly enters the large intestine, where it can highly selectively provide a nutrient matrix for beneficial bacteria such as bifidobacteria in the intestine, rapidly promote the mass reproduction of these beneficial bacteria, enhance their vitality, and thus improve the intestinal microecological environment.

[0037] The whitening and anti-aging composition of the present invention particularly incorporates two Chinese medicinal ingredients, namely, Panax notoginseng powder and edible spirulina powder. Panax notoginseng powder contains abundant physiologically active substances, including flavonoids, saponins and amino acids, which can effectively remove free radicals in the body, delay aging, and have a significant improvement effect on the skin, making the skin smoother and more elastic. The antioxidants contained in the spirulina powder can neutralize free radicals, reduce cell damage, help delay aging, and keep the skin young and healthy. In addition, the carotene and water-soluble dietary fiber in the spirulina powder can also prevent dry and rough skin, exert special skin care effects, and help improve the balance of intestinal flora. By combining these Chinese medicinal ingredients with other raw materials such as probiotics, the whitening and anti-aging composition of the present invention can improve both the inside and the outside, improve the skin condition, and regulate the intestinal microbial environment, achieving the dual effects of internal regulation and external nourishment.

[0038] The advantages or beneficial effects of the above technical solution include at least:

[0039] 1) The present invention combines three probiotics, namely, Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus salivarius, to prepare a composite probiotic. This composite probiotic can not only work synergistically to improve the body's antioxidant capacity, but also improve the metabolic activity of the intestinal microbiota. In the microencapsulation process, we creatively use the composite probiotic as the core material, and use calcium alginate modified with dopamine and hyaluronic acid as the inner layer, and maltodextrin modified with syringic acid as the outer layer. This structural design makes the inner core surface of the microcapsule more dense, effectively prevents the leakage of probiotics, and improves the encapsulation efficiency of probiotics. At the same time, this microcapsule structure can also protect the probiotics from being destroyed by gastric acid and bile salts, ensuring that the probiotics can safely reach the intestines and exert their effects.

[0040] 2) The invention cleverly combines fish collagen peptides and mannose, and combines the traditional Chinese medicine ingredients Panax notoginseng powder and edible spirulina powder. This combination can not only effectively prevent dry and rough skin, but also achieve a special skin care effect. In addition, these ingredients can promote the balance of intestinal flora, achieving a comprehensive health effect from internal regulation to external care.

[0041] 3) Vitamin C added in the present invention also has the ability to reduce free radicals, which helps to reduce cell damage and delay the aging process of the skin. At the same time, vitamin C is also involved in the synthesis of collagen, which is essential for maintaining the smoothness and beauty of the skin and fighting the aging phenomenon. In addition, due to its own antioxidant properties, starch can effectively protect the active ingredients in the whitening and anti-aging composition when used as a carrier to prevent them from being degraded due to oxidation. DETAILED DESCRIPTION

[0042] The technical solution of the present invention is further described below by specific implementation methods. However, the following examples are only simplified examples of the present invention and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention shall be subject to the claims.

[0043] Experimental Materials:

[0044] The Lactobacillus rhamnosus described in the present invention was purchased from the General Microbiological Center of China National Microbiological Culture Collection Administration, with a collection number of CGMCC No.28164.

[0045] The Bifidobacterium longum described in the present invention was purchased from the General Microbiological Center of China National Microbiological Culture Collection Administration, with a collection number of CGMCC NO. 14168.

[0046] The Lactobacillus salivarius described in the present invention was purchased from the General Microbiology Center of China National Microbiological Culture Collection Administration, with the collection number being CGMCC No.23518.

[0047] The invention inoculates the Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus salivarius into fermentation medium respectively to obtain fermentation liquid; then centrifuges the obtained fermentation liquid, collects the bacterial bodies, freezes and dries them to obtain Lactobacillus rhamnosus powder, Bifidobacterium longum powder and Lactobacillus salivarius respectively.

[0048] Unless otherwise specified, a component (such as fish collagen peptide or edible spirulina) in the parallel embodiments and comparative examples of the present invention is the same commercially available product. The dosage of the components in the following examples is 1 g per weight portion or per portion unless otherwise specified.

[0049] 1. Examples A1-A10 are methods for preparing freeze-dried composite probiotic powder.

[0050] The preparation method of Example A1 comprises the following steps:

[0051] S1. Dissolve 10 g of hyaluronic acid in 100 g of deionized water, then add 1.5 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and 2.0 g of N-hydroxysuccinimide, and stir at 35° C. for 2 h to prepare a hyaluronic acid mixed solution.

[0052] S2, 5g of sodium alginate and 1.5g of soy protein were mixed in 100g of deionized water, and the mixture was uniformly dispersed by ultrasonic treatment, followed by sterilization, and then 2.5g of composite microbial powder was added and stirred sufficiently to prepare a uniform composite microbial powder suspension; the ultrasonic conditions were as follows: the ultrasonic power was 300W, the ultrasonic room temperature was 35°C, and the ultrasonic time was 15min; the composite microbial powder was a mixture of Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder in a mass ratio of 0.5:1:2; the effective viable count of the Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder was 1×10 11 cfu / g.

[0053] S3. Synchronously drop the hyaluronic acid mixed solution prepared in step S1 and the composite microbial powder suspension obtained in step S2 into a 2wt% calcium chloride solution, stir to react for 1 hour, then drop a dopamine hydrochloride aqueous solution to react for 3 hours, wash, and freeze-dry at -60°C for 2 hours to obtain a microcapsule core material; the mass ratio of the hyaluronic acid mixed solution, the composite microbial powder suspension, and the dopamine hydrochloride aqueous solution is 1:5:3, wherein the mass concentration of dopamine hydrochloride in the dopamine hydrochloride aqueous solution is 3%.

[0054] S4. Mix and dissolve saccharide and maltodextrin in water to form a coating material solution of 5wt% saccharide and 15wt% maltodextrin, and then use this solution to atomize and coat the microcapsule core material obtained in step S3, and finally freeze-dry to obtain a composite probiotic freeze-dried powder; the conditions for atomization coating are: atomization pressure 0.5 MPa, air inlet temperature 50°C, air outlet temperature 25°C, coating chamber temperature 40°C, and the flow rate of the coating material solution is set to 0.5 mL / min.

[0055] The preparation method of Example A2 comprises the following steps:

[0056] S1. Take 5 g of hyaluronic acid and dissolve it in 100 g of deionized water, then add 0.5 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and 1.0 g of N-hydroxysuccinimide, and stir at 40° C. for 1 h to prepare a hyaluronic acid mixed solution.

[0057] S2, 3g of sodium alginate and 2.0g of soy protein were mixed in 100g of deionized water, and the mixture was uniformly dispersed by ultrasonic treatment, followed by sterilization, and then 1.5g of composite microbial powder was added and stirred sufficiently to prepare a uniform composite microbial powder suspension; the ultrasonic conditions were: ultrasonic power of 400W, ultrasonic room temperature of 35°C, and ultrasonic time of 10min; the composite microbial powder was a mixture of Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder in a mass ratio of 1:1:1; the effective viable count of the Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder was 5×10 10 cfu / g.

[0058] S3. Synchronously drop the hyaluronic acid mixed solution prepared in step S1 and the composite microbial powder suspension obtained in step S2 into a 3wt% calcium chloride solution, stir to react for 1h, then drop dopamine hydrochloride aqueous solution to react for 3h, wash, and freeze-dry at -65°C for 1.5h to obtain a microcapsule core material; the mass ratio of the hyaluronic acid mixed solution, the composite microbial powder suspension, and the dopamine hydrochloride aqueous solution is 2:5:2, wherein the mass concentration of dopamine hydrochloride in the dopamine hydrochloride aqueous solution is 2%.

[0059] S4. Mix and dissolve saccharide and maltodextrin in water to form a coating material solution of 8 wt% saccharide and 20 wt% maltodextrin, and then use this solution to atomize and coat the microcapsule core material obtained in step S3, and finally freeze-dry to obtain a composite probiotic freeze-dried powder; the coating conditions are: atomization pressure 0.1 MPa, air inlet temperature 55°C, air outlet temperature 35°C, coating chamber temperature 45°C, and the flow rate of the coating material solution is set to 0.8 mL / min.

[0060] The preparation method of Example A3 comprises the following steps:

[0061] S1. Dissolve 2 g of hyaluronic acid in 100 g of deionized water, then add 0.4 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and 0.2 g of N-hydroxysuccinimide, and stir at 50° C. for 1.5 h to prepare a hyaluronic acid mixed solution.

[0062] S2, 8g of sodium alginate and 1.0g of soy protein were mixed in 100g of deionized water, and the mixture was uniformly dispersed by ultrasonic treatment, followed by sterilization, and then 2.0g of composite microbial powder was added and stirred sufficiently to prepare a uniform composite microbial powder suspension; the ultrasonic conditions were: ultrasonic power of 350W, ultrasonic room temperature of 30°C, and ultrasonic time of 15min; the composite microbial powder was a mixture of Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder in a mass ratio of 1:2:2; the effective viable count of the Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder was 8×10 9 cfu / g.

[0063] S3. Synchronously drop the hyaluronic acid mixed solution prepared in step S1 and the composite microbial powder suspension obtained in step S2 into a 1wt% calcium hydrogen phosphate solution, stir to react for 1 hour, then drop a dopamine hydrochloride aqueous solution to react for 3 hours, wash, and freeze-dry at -60°C for 3 hours to obtain a microcapsule core material; the mass ratio of the hyaluronic acid mixed solution, the composite microbial powder suspension, and the dopamine hydrochloride aqueous solution is 2:10:3, wherein the mass concentration of dopamine hydrochloride in the dopamine hydrochloride aqueous solution is 3%.

[0064] S4. Mix and dissolve saccharide and maltodextrin in water to form a coating material solution of 2wt% saccharide and 18wt% maltodextrin, and then use this solution to atomize and coat the microcapsule core material obtained in step S3, and finally freeze-dry to obtain a composite probiotic freeze-dried powder; the coating conditions are: atomization pressure 0.3 MPa, air inlet temperature 50°C, air outlet temperature 40°C, coating chamber temperature 45°C, and the flow rate of the coating material solution is set to 0.6 mL / min.

[0065] The preparation method of Example A4 comprises the following steps:

[0066] S1. Dissolve 6 g of hyaluronic acid in 100 g of deionized water, then add 0.5 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and 0.6 g of N-hydroxysuccinimide, and stir at 30° C. for 2.5 h to prepare a hyaluronic acid mixed solution.

[0067] S2, 4g of sodium alginate and 2.5g of soy protein were mixed in 100g of deionized water, and the mixture was uniformly dispersed by ultrasonic treatment, followed by sterilization, and then 2.0g of composite microbial powder was added and stirred sufficiently to prepare a uniform composite microbial powder suspension; the ultrasonic conditions were as follows: the ultrasonic power was 400W, the ultrasonic room temperature was 25°C, and the ultrasonic time was 10min; the composite microbial powder was a mixture of Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder in a mass ratio of 0.5:2:2; the effective viable count of the Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder was 1×10 12 cfu / g.

[0068] S3. Synchronously drop the hyaluronic acid mixed solution prepared in step S1 and the composite microbial powder suspension obtained in step S2 into a 1wt% calcium chloride solution, stir and react for 2h, then drop a dopamine hydrochloride aqueous solution and react for 4h, wash, and freeze-dry at -60°C for 2h to obtain a microcapsule core material; the mass ratio of the hyaluronic acid mixed solution, the composite microbial powder suspension, and the dopamine hydrochloride aqueous solution is 1:8:3, wherein the mass concentration of dopamine hydrochloride in the dopamine hydrochloride aqueous solution is 4%.

[0069] S4. Mix and dissolve saccharide and maltodextrin in water to form a coating material solution of 5wt% saccharide and 16wt% maltodextrin, and then use this solution to atomize and coat the microcapsule core material obtained in step S3, and finally freeze-dry to obtain a composite probiotic freeze-dried powder; the coating conditions are: atomization pressure 0.3 MPa, air inlet temperature 55°C, air outlet temperature 40°C, coating chamber temperature 45°C, and the flow rate of the coating material solution is set to 0.5 mL / min.

[0070] The preparation method of Example A5 comprises the following steps:

[0071] Difference from Example A1: Step S1 of the hyaluronic acid mixed solution and Step S3 of the dopamine hydrochloride aqueous solution are omitted; the rest is the same as Example A1.

[0072] The preparation method of Example A6 comprises the following steps:

[0073] Difference from Example A1: The atomization coating treatment in step S4 is omitted, and the rest is the same as Example A1.

[0074] The preparation method of Example A7 comprises the following steps:

[0075] Difference from Example A1: In step S4, sialic acid is omitted and only maltodextrin is used as the coating solution. The rest is the same as Example A1.

[0076] The preparation method of Example A8 comprises the following steps:

[0077] The difference from Example A1 is that only Lactobacillus salivarius powder is used, and the rest is the same as Example A1.

[0078] The preparation method of Example A9 comprises the following steps:

[0079] The difference from Example A1 is that only Lactobacillus rhamnosus powder is used, and the rest is the same as Example A1.

[0080] The preparation method of Example A10 comprises the following steps:

[0081] The difference from Example A1 is that only Bifidobacterium longum powder is used, and the rest is the same as Example A1.

[0082] 2. Examples B1-B19 are whitening and anti-aging compositions, and the preparation thereof comprises the following steps:

[0083] The raw materials in Table 1 or Table 2 are mixed to obtain a premix, and then the premix is ​​mixed with auxiliary materials to prepare a whitening and anti-aging composition.

[0084] The auxiliary materials in Examples B1-B19 all consist of 5 parts of xylo-oligosaccharides, 3 parts of carrot powder, 1 part of orange flavor, 1 part of aspartame, 2 parts of caramel color and 8 parts of sodium carboxymethyl starch.

[0085] Table 1 Raw material composition of Examples B1-B8

[0086]

[0087] Table 2 Raw material composition of Examples B9-B19

[0088]

[0089] Performance evaluation:

[0090] Experimental Example 1: Simulated Gastric and Intestinal Fluid Test

[0091] 1) Simulate the preparation of gastric juice when full: First, prepare 2 grams of NaCl and 3.2 grams of pepsin. Then, add 7 milliliters of 36.5% hydrochloric acid, and then add water to 1000 milliliters.

[0092] 2) Preparation of simulated intestinal fluid: Take 6.8g of potassium dihydrogen phosphate, add 500ml of water to dissolve it, and adjust the pH value to 6.8 with 0.1mol / L sodium hydroxide solution; take another 10g of pancreatic enzyme, add appropriate amount of water to dissolve it, mix the two liquids, and dilute with water to 1000ml.

[0093] The composite probiotic freeze-dried powder obtained in Examples A1-A10 was taken separately and divided into three groups. In the first group, 1.0 g was taken from each sample in 10 mL of simulated gastric juice, shaken in a water bath shaker at 37 ° C for 30 min, taken out, and the number of viable bacteria was calculated by the coating plate method. In the second group, 1.0 g was taken from each sample in 10 mL of simulated gastric juice, shaken in a water bath shaker at 37 ° C for 60 min, taken out, and the number of viable bacteria was calculated by the coating plate method. In the third group, 1.0 g was taken from each sample in 10 mL of simulated gastric juice, shaken in a water bath shaker at 37 ° C for 60 min, 70 mL of simulated intestinal juice was added, and the culture was continued at 37 ° C for 2 h. Samples were taken from each sample and the number of viable bacteria was calculated by the coating plate method, and the viable bacteria rate was calculated. Wherein the viable bacteria rate (%) = the number of viable bacteria after passing through gastric juice (or intestinal juice) / the number of viable bacteria before passing through gastric juice (or intestinal juice) * 100%.

[0094] According to the test results of the third group, after the health care product composition of the present invention was treated in simulated intestinal fluid for 2 hours, there were basically no solid particles in the simulated intestinal fluid, and the microcapsules were basically completely lysed, indicating that the probiotics encapsulated on the surface and inside of the microcapsules were fully released and had good enteric solubility.

[0095] The results are shown in Table 3

[0096] Table 3

[0097] Experimental example 30min gastric juice viable bacteria rate / % 60min gastric juice viable bacteria rate / % Live bacteria rate after intestinal fluid / % A1 98.89 97.64 95.34 A2 98.23 97.06 94.77 A3 98.99 97.52 94.82 A4 99.12 97.76 95.16 A5 78.89 53.28 49.88 A6 65.34 45.34 40.52 A7 81.36 61.74 51.08 A8 98.82 97.78 95.66 A9 98.91 97.66 94.85 A10 97.75 95.98 94.03 .

[0098] According to the results in Table 3, it can be seen that the composite probiotic microcapsule freeze-dried powder of Examples A1-A4 prepared by the method of the present invention can survive in simulated gastric fluid at 37°C for 60 minutes, and the survival rate is more than 97%. This result shows that the microcapsule wall material layer used in the present invention can effectively protect the probiotics within 60 minutes, so that it has good gastric fluid tolerance. However, for Example A5, which is not wrapped with hyaluronic acid-dopamine, the density of the inner layer of its microcapsule is insufficient and the wrapping rate is low; while Example A6 does not use maltodextrin modified with syringic acid, and the outer layer of the microcapsule of Example A7 is only coated with maltodextrin, lacking the protection of syringic acid, resulting in the microcapsules of Examples A6 to A7 being more susceptible to gastric acid erosion, thereby significantly reducing the survival rate of probiotics. In a simulated intestinal fluid environment, the wall material layer of the microcapsule of the present invention can achieve rapid release of the fixed probiotics, and the survival rate of the bacterial agent is more than 94%. The experimental results of the comprehensive simulated gastric fluid environment show that the microcapsules prepared by the present invention can effectively deliver the embedded probiotics to the gastrointestinal tract to exert probiotic effects.

[0099] Experimental Example 2: Evaluation of Whitening Efficacy

[0100] First, 200 volunteers were recruited, with 10 people in each group, of which groups 1-19 were experimental groups taking Examples B1-B19, and group 20 was a control group (referred to as B20) without treatment. The subjects in the experimental group took 2g of the corresponding solid composition with warm water at 20:00 every evening for 8 consecutive weeks. During the experiment, the subjects could not use skin care products with whitening and skin color improvement effects and take other health care products with the above effects. Then the ColorimeterCL400 skin color test probe was used to measure the L*, a*, and b* values ​​of the area above the middle of the eyebrows, respectively. Each area was tested five times, and the ITA° value was recorded and calculated. The greater the test value of the whiteness of the skin ITA° in the facial test area, the lighter the skin color; the greater the value of the spot L* value in the facial test area, the brighter the skin color; the greater the measured value of the skin glossiness in the facial test area, the more shiny the skin.

[0101] Among them, the change rate of ITA° value on the 28th day (%) = (ITA° value on the 28th day - ITA° value on the 0th day) / ITA° value on the 0th day * 100%. The change rate of ITA° value on the 56th day and the change rate of L* value are calculated in the same way.

[0102] Table 4 Whitening effect evaluation results

[0103]

[0104] According to the data in Table 4, after the subjects used the compositions B1-B8 of the present invention within the 8 weeks of the test, the ITA° value showed an upward trend, with an increase of more than 8%; the skin brightness L* value also increased significantly, with an increase of more than 14%. This shows that the health care product composition of the present invention has the effect of improving skin brightness and increasing skin whiteness.

[0105] In contrast, Example B9 did not use hyaluronic acid-dopamine coated probiotic freeze-dried powder, Example B10 did not use maltodextrin modified with syringic acid, and the outer layer of the microcapsules of Example B11 was only coated with maltodextrin, lacking the protection of syringic acid. As a result, the probiotic microcapsules in these examples were eroded by gastric acid in gastric juice, and the survival rate of the probiotics was reduced. As a result, the increasing trend of the ITA° value and the L* value was not obvious, and the skin brightness and whiteness could not be significantly improved.

[0106] In addition, Examples B12-B14 only use one of Lactobacillus salivarius powder, Lactobacillus rhamnosus powder or Bifidobacterium longum powder, lacking antioxidant properties or probiotics that improve intestinal microbiota, and cannot achieve the function of internal regulation and external supplementation, resulting in poor whitening effect. Examples B15-B19 lack one of fish collagen peptide, edible spirulina powder, Panax notoginseng powder, vitamin C or mannose, resulting in no obvious increase in ITA° value and L* value.

[0107] Experimental Example 3: Evaluation of Anti-aging Effect

[0108] Detection method: First, the health care product composition of the present invention is treated in simulated gastric fluid for 1 hour, and then placed in simulated intestinal fluid for 2 hours. When there are basically no solid particles in the simulated intestinal fluid and the microcapsules are basically completely lysed, it is used as the test solution. 0.05mg / ml DPPH solution (1,1-diphenyl-2-trinitrophenylhydrazine) is prepared with anhydrous ethanol; 2mL of the pre-prepared test sample and 2mL of DPPH solution are placed in a test tube, and the test tube is placed in a constant temperature box at 37°C for 1 hour. Anhydrous ethanol is used as a blank control, and the absorbance A1 of each test sample is measured at 517nm, and the free radical scavenging rate is calculated according to the following formula: free radical scavenging rate = [1-(A1-A2) / A3]×100%. Among them, A3: absorbance of 2 mL of anhydrous ethanol plus 2 mL of DPPH solution; A1: absorbance of 2 mL of the test agent plus 2 mL of DPPH solution; A2: absorbance of 2 mL of the test agent plus 2 mL of anhydrous ethanol solution; three sets of parallel samples were measured for each test sample, and the average value was calculated. The results are shown in Table 5.

[0109] The ABTS free radical scavenging ability, superoxide anion free radical scavenging ability and hydroxyl free radical scavenging ability were determined according to the instructions of the ABTS free radical scavenging ability kit, the superoxide anion free radical ability kit and the hydroxyl free radical scavenging ability kit (purchased from Beijing Solebow Technology Co., Ltd.).

[0110] Table 5 Evaluation of anti-aging efficacy

[0111] Experimental example DPPH free radical scavenging rate / % Superoxide anion free radical scavenging rate / % Hydroxyl radical removal rate / % ABTS free radical removal rate / % Example B1 93.87 88.64 86.67 90.12 Example B2 94.35 89.32 85.98 89.54 Example B3 94.68 87.95 87.66 90.36 Example B4 95.10 88.47 86.91 89.78 Example B5 93.68 87.18 86.74 88.96 Example B6 94.49 88.61 85.47 90.31 Example B7 93.88 87.39 86.28 88.72 Example B8 95.26 88.73 87.33 89.06 Example B9 60.64 52.48 51.33 52.15 Example B10 58.46 49.47 48.65 49.97 Example B11 65.86 58.16 55.91 56.15 Example B12 73.24 68.18 65.44 66.88 Example B13 71.67 65.84 62.16 63.42 Example B14 72.33 63.66 60.62 60.95 Example B15 75.62 70.91 68.69 60.33 Example B16 76.34 74.22 70.78 61.47 Example B17 75.45 73.48 69.45 59.48 Example B18 77.94 76.68 74.61 62.44 Example B19 76.47 73.49 69.34 60.64

[0112] According to the data in Table 5, the health care product composition of the present invention exhibits significant antioxidant activity, which indicates that it has excellent antioxidant and anti-aging effects. However, the probiotic microcapsules in Examples B9-B11, due to the erosion of gastric acid, cause the probiotic survival rate to decrease, thereby reducing the number of probiotics that can enter the intestinal fluid, and Examples B12-B14 only use one of Lactobacillus salivarius powder, Lactobacillus rhamnosus powder or Bifidobacterium longum powder, and fail to achieve effective regulation of intestinal microbiota. These factors together lead to a significant decrease in these embodiments in DPPH free radical scavenging ability, ABTS free radical scavenging ability, superoxide anion free radical scavenging ability and hydroxyl free radical scavenging ability, thereby affecting their antioxidant and anti-aging effects.

[0113] In the composition of the embodiment of the present invention, fish collagen peptide, vitamin C and mannose all have antioxidant and anti-aging effects, and combined with the Chinese medicinal ingredients Panax notoginseng powder and edible spirulina powder, the effect of both internal regulation and external nourishment is achieved. In contrast, due to the lack of one or more key ingredients in fish collagen peptide, edible spirulina powder, Panax notoginseng powder, vitamin C or mannose, the ability of scavenging free radicals in Examples B15-B19 is reduced, thereby affecting the overall antioxidant and anti-aging effects.

[0114] The present invention has been described in detail through the general description, specific embodiments and experimental part. However, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, any such modifications or improvements made without departing from the core spirit of the present invention should be regarded as within the scope of the protection claimed by the present invention.

Claims

1. A whitening and anti-aging composition, characterized in that: The invention is composed of the following components by weight: 3-10 parts of fish collagen peptide, 1-8 parts of compound probiotic freeze-dried powder, 5-15 parts of edible spirulina powder, 10-15 parts of notoginseng powder, 5-10 parts of vitamin C, 3-8 parts of mannose and 10-20 parts of starch; The composite probiotic freeze-dried powder is prepared by a preparation method comprising the following steps: S1, dissolving hyaluronic acid in deionized water, then adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide, stirring evenly to prepare a hyaluronic acid mixed solution; S2, mixing sodium alginate and soy protein in deionized water, ultrasonically dispersing, and sterilizing, adding composite microbial powder, stirring evenly, and preparing a composite microbial powder suspension; the composite microbial powder is a mixture of Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder in a mass ratio of 0.5-1:1-2:1-2; the strain collection number of the Lactobacillus salivarius powder is CGMCC No.23518, the strain collection number of the Lactobacillus rhamnosus powder is CGMCCNo.28164, and the strain collection number of the Bifidobacterium longum powder is CGMCC NO. 14168; S3, synchronously dropping the hyaluronic acid mixed solution of step S1 and the composite microbial powder suspension of step S2 into the calcium salt solution, stirring for reaction for 1-3 hours, then dropping dopamine hydrochloride aqueous solution for reaction for 1-5 hours, washing, and freeze-drying to obtain the microcapsule core material; S4, mixing saccharide and maltodextrin and dissolving them in water to form a coating solution of 2-10wt% saccharide and 10-20wt% maltodextrin, using the coating solution to atomize and coat the microcapsule core material obtained in step S3, freeze-drying, and obtaining a composite probiotic freeze-dried powder.

2. The whitening and anti-aging composition according to claim 1, characterized in that: The mass concentration of hyaluronic acid in the mixed solution of step S1 is 1-10%; the mass content of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide relative to hyaluronic acid is 5-20%; the stirring temperature in step S1 is 30-50° C., and the stirring time is 1-3h.

3. The whitening and anti-aging composition according to claim 1, characterized in that: The sodium alginate concentration in the suspension of step S2 is 3-8wt%; the soy protein concentration is 1-3.5wt%; the ultrasonic conditions are: ultrasonic power is 300-400W, ultrasonic room temperature is 20-35°C, and ultrasonic time is 10-15min.

4. The whitening and anti-aging composition according to claim 1, characterized in that: The mass concentration of the bacterial powder in the suspension of step S2 is 1-3.5%; the effective viable counts of the Lactobacillus salivarius powder, Lactobacillus rhamnosus powder, and Bifidobacterium longum powder are all 1×10 9 cfu / g-1×10 12 cfu / g.

5. The whitening and anti-aging composition according to claim 1, characterized in that: In the step S3, the mass ratio of the hyaluronic acid mixed solution, the composite microbial powder suspension, and the dopamine hydrochloride aqueous solution is 1-2:5-10:1-3, wherein the mass concentration of dopamine hydrochloride in the dopamine hydrochloride aqueous solution is 1-5%; in the step S3, the stirring reaction conditions are: the reaction temperature is 20-40°C, and the reaction time is 1-3h; in the step S3, the calcium salt solution includes any one of calcium gluconate, calcium hydrogen phosphate or calcium chloride, and the mass concentration of the calcium salt is 1-5%; the freeze-drying conditions of the step S3 are: freeze-drying at -70 ~ -60°C for 2-3h.

6. The whitening and anti-aging composition according to claim 1, characterized in that: In step S4, the conditions for atomization coating are: atomization pressure 0.1-0.5 MPa, air inlet temperature 50-60°C, air outlet temperature 25-40°C, coating chamber temperature 40-50°C; the flow rate of the coating material solution is set to 0.3 mL / min-1.0 mL / min.

7. The whitening and anti-aging composition according to claim 1, characterized in that: The composition further comprises 10-30 parts of food-acceptable auxiliary materials, wherein the auxiliary materials comprise one or more of dietary fiber, fruit and vegetable powder, edible flavor, flavoring agent, colorant and excipient.

8. The whitening and anti-aging composition according to claim 7, characterized in that: The dietary fiber is at least one of fructooligosaccharides, xylooligosaccharides, galacto-oligosaccharides, isomaltooligosaccharides and oats; The fruit and vegetable powder is one or a mixture of two or more of orange powder, lemon powder, carrot powder, purple potato powder, yam powder, spinach powder or coconut powder; The edible flavor is one or a mixture of two or more of orange flavor, orange flavor, lemon flavor, cherry flavor, menthol, apple flavor or coconut flavor; The flavoring agent is one or a mixture of two or more of sucralose, AK sugar, aspartame or mogroside; The toner is one or a mixture of two or more of caramel color, gardenia yellow, curcumin or chlorophyll; The excipient is one or a mixture of two or more of sodium carboxymethyl starch, xanthan gum or sodium carboxymethyl cellulose.

9. The whitening and anti-aging composition according to any one of claims 1 to 8, characterized in that: The composition is one of powder, tablet, granule and oral liquid.

10. The method for preparing the whitening and anti-aging composition according to any one of claims 1 to 6 and 9, characterized in that: The following steps are involved: 3-10 parts of fish collagen peptide, 1-8 parts of compound probiotic freeze-dried powder, 5-15 parts of edible spirulina powder, 10-15 parts of Panax notoginseng powder, 5-10 parts of vitamin C, 3-8 parts of mannose and 10-20 parts of starch are mixed and processed to prepare a whitening and anti-aging composition.

11. The method for preparing the whitening and anti-aging composition according to any one of claims 7 to 9, characterized in that: The following steps are involved: 3-10 parts of fish collagen peptide, 1-8 parts of compound probiotic freeze-dried powder, 5-15 parts of edible spirulina powder, 10-15 parts of Panax notoginseng powder, 5-10 parts of vitamin C, 3-8 parts of mannose and 10-20 parts of starch are mixed to obtain a premix, and then the premix is ​​mixed with auxiliary materials to prepare a whitening and anti-aging composition.

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