An organic fertilizer for increasing yield of planting guavas in saline-alkali soil and a preparation method thereof

Abstract

This invention discloses an organic fertilizer for increasing guava yield in saline-alkali land and its preparation method, relating to the field of biotechnology. The active ingredients of this organic fertilizer include *Bacillus horikoshii* ACCC 01061, *Bacillus safensis* CICC 21743, *Sporidiobolus salmonicolor* CICC 32895, *Trichoderma koningii* CICC 13006, and *Trichoderma longibrachiatum* ACCC 32095. This organic fertilizer can improve the yield per guava tree and the quality of the fruit grown in saline-alkali land, and has significant market application value.

Description

An organic fertilizer for increasing guava yield in saline-alkali land and its preparation method Technical Field

[0001] This invention relates to the field of biotechnology, and in particular to an organic fertilizer for increasing the yield of guava grown in saline-alkali land and its preparation method. Background Technology

[0002] Guava (scientific name: *Psidium guajava* L.), also known as guava, chicken-dung fruit, trumpet guava, etc., is a tree belonging to the genus *Psidium* in the family Myrtaceae. Native to tropical America, guava has since spread widely throughout tropical and subtropical regions worldwide, and was introduced to my country around the late 17th century. Guava fruit is rich in nutrients and is considered one of the best fruits for beauty, skin care, and weight loss. The fruit can be eaten fresh or processed into juice, fruit powder, and other products. Guava also has certain medicinal, landscaping, and ornamental value.

[0003] Saline-alkali soils have relatively low organic matter content, which inhibits the process of soil herbicide formation and organic matter accumulation, leading to poor soil nutrient conditions. Saline-alkali soils also have high pH values ​​and high calcium carbonate content, causing phosphorus in the soil to form insoluble phosphates, reducing phosphorus availability. Furthermore, saline-alkali soils have poor physical properties, with poor moisture retention and permeability, affecting normal crop growth. Planting guava in saline-alkali soil easily leads to flower and fruit drop, reduced fruit yield, decreased fruit quality, and significant economic losses.

[0004] This invention aims to develop an organic fertilizer suitable for use in saline-alkali land to improve the yield and quality of guava. Summary of the Invention

[0005] The purpose of this invention is to provide an organic fertilizer and its preparation method for increasing the yield of guava grown in saline-alkali land, thereby solving the problems existing in the prior art. This organic fertilizer can improve the yield per guava tree and the quality of the fruit grown in saline-alkali land, and has significant market application value.

[0006] To achieve the above objectives, the present invention provides the following solution:

[0007] This invention provides a microbial combination for increasing guava yield in saline-alkali land, comprising Bacillus horikoshii ACCC 01061, Bacillus safensis CICC 21743, Sporidiobolus salmonicolor CICC 32895, Trichoderma koningii CICC 13006, and Trichoderma longibrachiatum ACCC 32095.

[0008] The present invention also provides the application of the above-mentioned microbial combination in the preparation of microbial agents, which are used to improve the yield and / or fruit quality of guava grown in saline-alkali land.

[0009] The present invention also provides a microbial agent for improving the yield and / or fruit quality of guava grown in saline-alkali land, wherein the active ingredients include the above-mentioned microbial combination.

[0010] The present invention also provides the application of the above-mentioned microbial combination or microbial agent in the preparation of organic fertilizers that improve the yield and / or fruit quality of guava grown in saline-alkali land.

[0011] The present invention also provides an organic fertilizer for improving the yield and / or fruit quality of guava grown in saline-alkali land, wherein the active ingredients include the above-mentioned microbial agents.

[0012] Furthermore, the organic fertilizer also includes a fertilizer carrier; the fertilizer carrier includes crop straw and attapulgite powder.

[0013] Crop straw can be selected from one or a combination of several of the following: corn straw, wheat straw, rice straw, sorghum straw, soybean straw, and cotton straw.

[0014] Furthermore, the mass ratio of the crop straw to the attapulgite powder is 7:2.

[0015] The present invention also provides a method for preparing the above-mentioned organic fertilizer, comprising the following steps:

[0016] Bacillus horikosa ACCC 01061, Bacillus safortus CICC 21743, Saccharomyces cerevisiae CICC 32895, Trichoderma corniglanum CICC 13006, and Trichoderma longicornis ACCC 32095 were fermented separately to obtain fermentation broths of Bacillus horikosa ACCC 01061, Bacillus safortus CICC 21743, Saccharomyces cerevisiae CICC 32895, Trichoderma corniglanum CICC 13006, and Trichoderma longicornis ACCC 32095.

[0017] The fermentation broths of *Bacillus horikosa* ACCC 01061, *Bacillus safortifolius* CICC 21743, *Saccharomyces cerevisiae* CICC 32895, *Trichoderma cornuta* CICC 13006, and *Trichoderma longicornis* ACCC 32095 were concentrated to a viable cell count of 10. 9 After CFU / mL, mix thoroughly at a live bacteria ratio of 1:2:1:1:1 to obtain a mixed bacterial solution;

[0018] Crop straw and attapulgite powder are mixed evenly at a mass ratio of 7:2 to obtain a fertilizer carrier.

[0019] The fertilizer carrier and the mixed bacterial solution are mixed evenly at a mass ratio of 9:1, and then granulated by extrusion to obtain the organic fertilizer.

[0020] The present invention also provides the application of the above-mentioned microbial combinations, microbial agents or organic fertilizers in improving the yield and / or fruit quality of guava grown in saline-alkali land.

[0021] The present invention also provides a method for improving the yield and / or fruit quality of guava grown in saline-alkali land, including the step of applying the above-mentioned microbial combination, microbial agent or organic fertilizer to guava trees.

[0022] The present invention discloses the following technical effects:

[0023] This invention develops an organic fertilizer to increase guava yield in saline-alkali land. It is prepared using five active microbial strains: *Bacillus horinosae* ACCC 01061, *Bacillus safoetida* CICC 21743, *Saccharomyces cerevisiae* CICC 32895, *Trichoderma cornuta* CICC 13006, and *Trichoderma longicornis* ACCC 32095. These five microbial strains exhibit a unique synergistic effect, optimizing the soil microbial community structure, promoting the production of organic acids, and enhancing soil enzyme activity, thereby increasing the yield per guava tree and improving fruit quality in saline-alkali land. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 shows the average yield per plant in each treatment group;

[0026] Figure 2 shows the statistical chart of VC content in each treatment group;

[0027] Figure 3 shows the statistical chart of soluble sugar content in each treatment group;

[0028] Figure 4 shows the statistical chart of solid content in each treatment group. Detailed Implementation

[0029] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0030] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0031] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0032] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0033] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0034] Yeast extract, also known as powdered yeast extract, is a biological culture medium product made from high-protein baker's yeast or brewer's yeast through processes such as autolysis, enzymatic hydrolysis, concentration, and drying. It is rich in nutrients including protein, amino acids, peptides, polypeptides, nucleic acids, vitamins, and trace elements. Yeast extract is highly nutritious and widely used in the microbial culture industry. Later, yeast extracts used in the microbial culture industry were called yeast paste (paste product) and yeast powder (powder product). Broadly speaking, yeast extract and yeast extractant are the same type of product. In the industry, yeast extractant specifically used as a biological fermentation medium is usually called yeast extract, while yeast extractant specifically used as a flavoring food ingredient is generally called yeast extractant.

[0035] The 2,6-dichlorophenolindophenol titration method is a commonly used method for determining vitamin C (VC) content. This method is based on the reducing power of vitamin C and the oxidizing power of 2,6-dichlorophenolindophenol. The specific principle is as follows:

[0036] Principle: Vitamin C has strong reducing properties, while 2,6-dichlorophenolindophenol has strong oxidizing properties. In acidic solutions, 2,6-dichlorophenolindophenol is red, and in neutral or alkaline solutions, it is blue. When an acidic solution containing ascorbic acid is titrated with a blue 2,6-dichlorophenolindophenol solution, the ascorbic acid reduces 2,6-dichlorophenolindophenol to a colorless substance. Once all the ascorbic acid in the solution has been oxidized, adding more 2,6-dichlorophenolindophenol will turn the solution red; this is the titration endpoint. The amount of 2,6-dichlorophenolindophenol consumed during the titration can be used to calculate the vitamin C content in the sample. The experimental steps are as follows:

[0037] Sample preparation: Weigh a certain amount of fruit or vegetable sample, add oxalic acid solution, grind and filter to obtain sample extract.

[0038] Dye standardization: Take standard ascorbic acid solution and titrate with 2,6-dichlorophenolindophenol solution until pink color appears and does not fade within 30 seconds. Calculate the number of milligrams of ascorbic acid equivalent to each milliliter of dye.

[0039] Titration of the sample: Take an appropriate amount of sample extract and titrate it with 2,6-dichlorophenolindophenol solution until it turns pink. Record the volume of dye consumed and calculate the vitamin C content in the sample based on the calibration results.

[0040] Application scope: This method is applicable to the determination of vitamin C content in fruits, vegetables and other foods.

[0041] The anthrone colorimetric method is used to determine the content of carbohydrates. Its basic principle is that carbohydrates react with anthrone reagent in the presence of concentrated sulfuric acid to produce a blue-green substance. The absorbance is measured colorimetrically to calculate the carbohydrate content. The experimental steps are as follows:

[0042] Reagent preparation:

[0043] Anthrone reagent: Dissolve 0.4g of anthrone in 100mL of 88% sulfuric acid.

[0044] Glucose, fructose, and sucrose standard solutions: Accurately weigh a certain amount of dried analytically pure sugar and prepare a solution of appropriate concentration.

[0045] Plot the standard curve:

[0046] Take standard sugar solutions of different concentrations, add anthrone reagent, heat and measure absorbance to plot standard curves.

[0047] Sample determination:

[0048] Take the sample solution to be tested, add anthrone reagent, heat under the same conditions, and then measure the absorbance.

[0049] The sugar content of the sample was calculated using a standard curve.

[0050] Applications: The anthrone colorimetric method is widely used to determine the soluble sugar content in fruits and vegetables, as well as the total water-soluble sugar content in plant tissues such as cotton. This method is characterized by its simplicity and accuracy, and is suitable for the quantitative analysis of various sugars.

[0051] The strains and culture media used in the following examples are as follows:

[0052] The LB liquid medium consists of the following components: 5 g / L yeast extract, 10 g / L tryptone, and 10 g / L sodium chloride, pH 7.2 (15 g / L agar powder is added to the solid medium).

[0053] The LB solid medium consists of the following components: 5 g / L yeast extract, 10 g / L tryptone, 10 g / L sodium chloride, and 15 g / L agar powder, pH 7.2.

[0054] The NA liquid culture medium consists of the following components: 10 g / L peptone, 3 g / L beef extract, and 5 g / L sodium chloride, pH 7.2.

[0055] The NA liquid culture medium consists of the following components: 10 g / L peptone, 3 g / L beef extract, 5 g / L sodium chloride, and 15 g / L agar powder, pH 7.2.

[0056] The PDA liquid culture medium consists of 200 g / L potato and 20 g / L glucose, with a natural pH.

[0057] The PDA solid culture medium consists of the following components: 200 g / L potato, 20 g / L glucose, and 15 g / L agar powder, with a natural pH.

[0058] The bacterial fermentation medium consists of the following components by weight percentage: 1.5% glucose, 1% peptone, 0.6% yeast extract, 0.2% potassium dihydrogen phosphate, 0.002% ferrous sulfate heptahydrate, 0.002% zinc sulfate heptahydrate, 0.05% manganese sulfate heptahydrate, 0.03% magnesium sulfate heptahydrate, and the balance being water, pH 7.2.

[0059] The yeast fermentation medium consists of the following components by weight percentage: 12.0% glucose, 2.0% brown sugar, 3.0% peptone, 1.0% yeast extract, 0.1% potassium dihydrogen phosphate, 1.0% dipotassium hydrogen phosphate, 0.1% magnesium sulfate heptahydrate, 0.1% sodium chloride, and the balance water, pH 6.0.

[0060] The composition of the mold fermentation medium by weight percentage is as follows: 3% soluble starch, 1% peptone, 1.5% soybean meal, 0.2% dipotassium hydrogen phosphate, 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate, and the balance water, pH 6.5.

[0061] Bacillus horikoshii ACCC 01061 was purchased from the China Agricultural Microbial Culture Collection Center (ACCC), strain number ACCC 01061;

[0062] Bacillus safensis CICC 21743 was purchased from the China Industrial Microbial Culture Collection Center, strain number CICC 21743;

[0063] Bacillus saffron CICC 25233 was purchased from the China Industrial Microbial Culture Collection Center, strain number CICC25233;

[0064] Sporidiobolus salmonicolor CICC 32895 was purchased from the China Industrial Microbial Culture Collection Center, strain number CICC 32895;

[0065] Trichoderma koningii CICC 13006 was purchased from the China Industrial Microbial Culture Collection Center, strain number CICC 13006;

[0066] Trichoderma longibrachiatum CICC 41491 was purchased from the China Industrial Microbial Culture Collection Center, strain number CICC 41491;

[0067] Trichoderma longifolia ACCC 32095 was purchased from the China Agricultural Microbial Culture Collection Center, strain number ACCC32095.

[0068] Example 1

[0069] 1. The preparation method of fermentation broth for Bacillus horikosa ACCC 01061 is as follows:

[0070] (1) Bacillus horikosa ACCC 01061 was inoculated into LB liquid medium and cultured at 37℃ with shaking at 180r / min for 16h to obtain Bacillus horikosa ACCC 01061 bacterial suspension;

[0071] (2) The Bacillus oryzae ACCC 01061 bacterial suspension obtained in step (1) was streaked onto LB solid medium plates and cultured in an incubator at 37°C for 48 hours.

[0072] (3) Pick a single colony from the plate in step (2) and inoculate it into an Erlenmeyer flask containing LB liquid medium. Incubate at 37°C and 180 r / min for 16 h with shaking to obtain seed culture.

[0073] (4) The seed culture obtained in step (3) was transferred to a fermenter containing bacterial fermentation medium at an inoculation rate of 5%. The culture was carried out at 37°C, 200 r / min, and a pressure of 0.05 MPa for 24 h to obtain the fermentation broth of Bacillus horikosa ACCC 01061. The broth was then concentrated to a viable cell count of 10%. 9 CFU / mL, for later use.

[0074] 2. The preparation method of fermentation broth for Bacillus sabovella CICC 21743 is as follows:

[0075] (1) Inoculate Bacillus salsa CICC 21743 into LB liquid medium and culture at 37℃ with shaking at 180r / min for 16h to obtain Bacillus salsa CICC 21743 bacterial suspension;

[0076] (2) The bacterial suspension of Bacillus salsa CICC 21743 obtained in step (1) was streaked onto LB solid medium plates and incubated in an incubator at 37°C for 48 hours;

[0077] (3) Pick a single colony from the plate in step (2) and inoculate it into an Erlenmeyer flask containing LB liquid medium. Incubate at 37°C and 180 r / min for 16 h with shaking to obtain seed culture.

[0078] (4) The seed culture obtained in step (3) was transferred to a fermenter containing bacterial fermentation medium at an inoculation rate of 5%. The culture was carried out at 37°C, 200 r / min, and a pressure of 0.05 MPa for 24 h to obtain the fermentation broth of Bacillus salsa CICC 21743. The broth was then concentrated to a viable cell count of 10%. 9 CFU / mL, for later use.

[0079] 3. The preparation method of fermentation broth for *Saccharomyces cerevisiae* CICC 32895 is as follows:

[0080] (1) Inoculate the salmon-colored yeast CICC 32895 into NA liquid medium and culture at 30℃ with shaking at 150r / min for 24h to obtain the salmon-colored yeast CICC 32895 bacterial culture;

[0081] (2) The *Saccharomyces cerevisiae* CICC 32895 bacterial culture obtained in step (1) was streaked onto NA solid medium plates and cultured in an incubator at 30°C for 48 hours.

[0082] (3) Pick a single colony from the plate in step (2) and inoculate it into an Erlenmeyer flask containing NA liquid culture medium. Incubate at 30℃ and 180r / min for 24h with shaking to obtain seed culture.

[0083] (4) The seed culture obtained in step (3) was transferred to a fermenter containing yeast fermentation medium at an inoculation rate of 5%. The fermentation was carried out at 30°C, 180 r / min, and a pressure of 0.05 MPa for 24 h to obtain the fermentation broth of *Saccharomyces cerevisiae* CICC 32895. The broth was then concentrated to a viable cell count of 10%. 9 CFU / mL, for later use.

[0084] 4. The preparation method of fermentation broth for Corning Trichoderma CICC 13006 is as follows:

[0085] (1) Pick a piece of Trichoderma Corning CICC 13006 and inoculate it into PDA liquid medium. Incubate at 28℃ and shake at 180r / min for 72h to obtain the culture solution.

[0086] (2) Dilute the culture medium obtained in step (1) and spread it on PDA solid culture medium, and incubate it in an incubator at 28°C for 5 days;

[0087] (3) Pick a single colony from the plate in step (2) and inoculate it into an Erlenmeyer flask containing PDA liquid culture medium. Incubate at 28°C and 200 r / min for 48 h with shaking to obtain seed culture.

[0088] (4) The seed culture obtained in step (3) was transferred to a mold fermentation medium and cultured continuously at 28℃, 220 r / min, and 0.05 MPa for 48 h to obtain the fermentation broth of Trichoderma Corning CICC 13006. The broth was then concentrated to a viable spore content of 10. 9 CFU / mL, for later use.

[0089] 5. The preparation method of fermentation broth for Trichoderma longifolia ACCC 32095 is as follows:

[0090] (1) Pick a piece of Trichoderma longibranchs ACCC 32095 and inoculate it into PDA liquid medium. Incubate at 28℃ with shaking at 180r / min for 72h to obtain the culture solution.

[0091] (2) Dilute the culture medium obtained in step (1) and spread it on PDA solid culture medium, and incubate it in an incubator at 28°C for 5 days;

[0092] (3) Pick a single colony from the plate in step (2) and inoculate it into an Erlenmeyer flask containing PDA liquid culture medium. Incubate at 28°C and 200 r / min for 48 h with shaking to obtain seed culture.

[0093] (4) The seed culture obtained in step (3) was transferred to a mold fermentation medium and cultured continuously at 28℃, 220 r / min, and 0.05 MPa for 48 h to obtain the fermentation broth of Trichoderma longifolia ACCC 32095. The broth was then concentrated to a viable spore content of 10. 9 CFU / mL, for later use.

[0094] 6. Preparation of organic fertilizer

[0095] A carrier is prepared using crop straw and attapulgite powder as raw materials, which is then mixed evenly with microbial agents and granulated to produce organic fertilizer. The crop straw can be one or a combination of several of the following: corn straw, wheat straw, rice straw, sorghum straw, soybean straw, and cotton straw. The following explanation uses rice straw as an example to illustrate the preparation process of organic fertilizer:

[0096] (1) The fermentation broths of Bacillus horikosa ACCC 01061, Bacillus saforus CICC 21743, Saccharomyces cerevisiae CICC32895, Trichoderma corniglansii CICC 13006 and Trichoderma longicornis ACCC 32095 were mixed evenly at a live bacteria (or spore) ratio of 1:2:1:1:1 to obtain a mixed bacterial solution.

[0097] (2) After crushing the rice straw, pass it through a 40-mesh sieve to obtain rice straw powder. Mix the rice straw powder and attapulgite powder evenly at a mass ratio of 7:2 to obtain a fertilizer carrier.

[0098] (3) Mix the fertilizer carrier and the mixed bacterial solution at a mass ratio of 9:1, and then granulate them by conventional extrusion to obtain granular organic fertilizer.

[0099] Comparative Example 1

[0100] Same as Example 1, except that Bacillus salsa CICC 21743 is replaced with Bacillus salsa CICC25233.

[0101] Comparative Example 2

[0102] Same as Example 1, except that Trichoderma longifolia ACCC 32095 is replaced with Trichoderma longifolia CICC 41491.

[0103] Comparative Example 3

[0104] Same as Example 1, except that Bacillus horinosae ACCC 01061 was removed when preparing the mixed bacterial solution.

[0105] Comparative Example 4

[0106] Same as Example 1, except that Bacillus salsa CICC 21743 was removed when preparing the mixed bacterial solution.

[0107] Comparative Example 5

[0108] Same as Example 1, except that when preparing the mixed bacterial solution, *Saccharomyces cerevisiae* CICC 32895 was removed.

[0109] Comparative Example 6

[0110] Same as Example 1, except that Corning Trichoderma CICC 13006 was removed when preparing the mixed bacterial solution.

[0111] Comparative Example 7

[0112] Same as Example 1, except that Trichoderma longifolia ACCC 32095 was removed when preparing the mixed bacterial solution.

[0113] Comparative Example 8

[0114] Same as Example 1, except that an equal amount of fermentation medium is used instead of the fermentation broth of each strain.

[0115] Effect verification example

[0116] 1. Test materials

[0117] The experimental field is located at the experimental demonstration base of the Guangdong Academy of Agricultural Sciences. The soil is saline-alkali with a salt content of 0.5% and an alkalinity of 18%.

[0118] Test material: 5-year-old pearl guava.

[0119] Compound fertilizer: Nitrogen, phosphorus and potassium 15-15-15 compound fertilizer.

[0120] 2. Experimental Design

[0121] Nine treatment groups were set up, with 20 guava trees planted in each group. The ring trench fertilization method was used, and fertilizer was applied to the guava trees in each treatment group in February and May, respectively. The type and amount of fertilizer applied to each tree are shown in Table 1.

[0122] Table 1. Types and amounts of fertilizer applied to different treatment groups.

[0123]

[0124]

[0125] 3. Indicator Testing

[0126] Multiple batches of mature fruits were harvested and weighed in each treatment group, and the yield per plant was then calculated. At maturity, 20 fruits were randomly collected from each treatment group, and the contents of vitamin C (VC), soluble sugar, and solids were determined. The VC content was determined by the 2,6-dichlorophenolindophenol titration method; the soluble sugar content was determined by the anthrone colorimetric method; and the solids content was determined by the refractometer method.

[0127] 4. Test Results

[0128] The average yield per plant and the contents of VC, soluble sugar, and solids in each treatment group are shown in Figures 1-4. The results show that, in addition to applying compound fertilizer, the application of the organic fertilizer prepared according to this invention can effectively increase the yield per guava plant. Furthermore, through strain replacement, it was found that *Bacillus saefolius* and *Trichoderma longicornis* used in this invention have certain unique characteristics; using other strains of *Bacillus saefolius* or *Trichoderma longicornis* did not achieve the expected results. This invention hypothesizes that there is a special synergistic effect among *Bacillus horikomi* ACCC 01061, *Bacillus saefolius* CICC 21743, *Saccharomyces cerevisiae* CICC 32895, *Trichoderma corniglita* CICC 13006, and *Trichoderma longicornis* ACCC 32095 used in this invention. Under their combined action, the soil microbial community structure can be optimized, promoting the production of organic acids by microorganisms and enhancing soil enzyme activity, thereby improving the yield per guava plant and fruit quality in saline-alkali land.

[0129] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A microbial ensemble for increasing guava yield in saline-alkali land, characterized in that, This includes Bacillus horikoshii (ACCC 01061), Bacillus safensis (CICC 21743), Sporidiobolus salmonicolor (CICC 32895), Trichoderma koningii (CICC 13006), and Trichoderma longibrachiatum (ACCC 32095).

2. The application of the microbial ensemble as described in claim 1 in the preparation of microbial inoculants, characterized in that, The microbial agent is used to improve the yield and / or fruit quality of guava grown in saline-alkali land.

3. A microbial inoculant for improving the yield and / or fruit quality of guava grown in saline-alkali land, characterized in that, The active ingredient includes the microbial ensemble described in claim 1.

4. The application of the microbial combination as described in claim 1 or the microbial agent as described in claim 3 in the preparation of organic fertilizer for improving the yield and / or fruit quality of guava grown in saline-alkali land.

5. An organic fertilizer for improving the yield and / or fruit quality of guava grown in saline-alkali land, characterized in that, The active ingredient includes the microbial agent as described in claim 3.

6. The organic fertilizer according to claim 5, characterized in that, The organic fertilizer also includes a fertilizer carrier; the fertilizer carrier includes crop straw and attapulgite powder.

7. The organic fertilizer according to claim 6, characterized in that, The mass ratio of the crop straw to the attapulgite powder is 7:

2.

8. A method for preparing organic fertilizer as described in claim 5, characterized in that, Includes the following steps: Bacillus horikosa ACCC 01061, Bacillus safortus CICC 21743, *Saccharomyces cerevisiae* CICC 32895, *Trichoderma cornuta* CICC 13006, and *Trichoderma longicornis* ACCC 32095 were fermented separately to obtain fermentation broths of Bacillus horikosa ACCC 01061, Bacillus safortus CICC 21743, *Saccharomyces cerevisiae* CICC 32895, *Trichoderma cornuta* CICC 13006, and *Trichoderma longicornis* ACCC 32095. The fermentation broths of Bacillus horikosa ACCC 01061, Bacillus safortus CICC 21743, *Saccharomyces cerevisiae* CICC 32895, *Trichoderma cornuta* CICC 13006, and *Trichoderma longicornis* ACCC 32095 were then concentrated to a viable cell count of 10. 9 After CFU / mL, the mixture is thoroughly mixed at a live bacteria ratio of 1:2:1:1:1 to obtain a mixed bacterial solution; crop straw and attapulgite powder are thoroughly mixed at a mass ratio of 7:2 to obtain a fertilizer carrier; the fertilizer carrier and the mixed bacterial solution are thoroughly mixed at a mass ratio of 9:1, and the mixture is granulated by extrusion to obtain the organic fertilizer.

9. The application of a microbial combination as described in claim 1, a microbial agent as described in claim 3, or an organic fertilizer as described in any one of claims 5-7 in improving the yield and / or fruit quality of guava grown in saline-alkali land.

10. A method for improving the yield and / or fruit quality of guava grown in saline-alkali land, characterized in that, The method includes the step of applying the microbial combination of claim 1, the microbial agent of claim 3, or the organic fertilizer of any one of claims 5-7 to guava trees.

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