An anti-aging skin care composition prepared by using algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate and its preparation process

Abstract

The present invention belongs to the field of cosmetics technology and specifically relates to an anti-aging skin care composition prepared using algal oligosaccharides, carboxymethyl chitosan, and hydrolyzed sodium hyaluronate. The anti-aging skin care composition comprises algal oligosaccharides, carboxymethyl chitosan, and hydrolyzed sodium hyaluronate. The present invention has discovered that the composition of algal oligosaccharides, carboxymethyl chitosan, and hydrolyzed sodium hyaluronate can promote the synthesis of type I, III, and IV collagen in fibroblasts. The three ingredients synergistically promote a significant increase in the synthesis of type I, III, and IV collagen, thereby achieving anti-aging, anti-wrinkle, and firming effects. Therefore, the composition can be used in anti-aging products.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to an anti-aging skin care composition prepared by utilizing algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, and a preparation process thereof. Background Art

[0002] Skin aging is a natural process. Over time, the skin gradually loses its ability to repair itself. Environmental damage damages elastin and collagen, reducing collagen production within the skin. This weakens the adhesion proteins that connect the dermis and epidermis, leading to a sharp drop in collagen production. This weakened collagen prevents the skin from storing energy and gradually reduces its ability to retain moisture. Skin aging primarily occurs in the dermis and manifests itself in the epidermis. Complex environmental factors such as ultraviolet light, air pollution, and nicotine can reduce the amount of interstitial tissue in the epidermis, slowing the division of keratin and accelerating keratinocyte aging and depression. The epidermis loses its luster, becomes rough and dull, and develops fine wrinkles. Reduced collagen fiber synthesis and collagen hydrolysis within the dermis lead to degeneration of elastic fibers in the reticular layer of the dermis, resulting in collagen loss, dermal atrophy, sagging, and deep wrinkles. The emergence of anti-wrinkle cosmetics has brought hope to those who desire younger-looking skin, satisfying their desire for youthful skin. While numerous anti-aging products exist on the market, most are derived from chemical synthesis methods, which is inconsistent with the current trend of pure beauty. While some botanical anti-aging products are available, their effectiveness is subpar or suffers from poor absorption. Therefore, there is an urgent need to develop effective anti-aging products with fewer chemical ingredients to meet people's demand for green skincare. Summary of the Invention

[0003] In order to solve the above problems, the present invention provides an anti-aging skin care composition prepared by using algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, which comprises algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate.

[0004] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6-10 parts, 0.3-0.8 parts and 5 parts respectively.

[0005] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6 parts, 0.8 parts and 5 parts respectively.

[0006] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6-10 parts, 3 parts and 5 parts respectively.

[0007] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 10 parts, 0.8 parts and 5 parts respectively.

[0008] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 10 parts, 3 parts and 5 parts respectively.

[0009] Furthermore, the anti-aging skin care composition further comprises auxiliary materials and preservatives.

[0010] Furthermore, the weight portion of the preservative in the anti-aging skin care composition is 0.3-0.6 parts.

[0011] Furthermore, the preservative includes but is not limited to any one of 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid, caprylyl glycol, pentylene glycol and ethylhexylglycerin.

[0012] Furthermore, the auxiliary materials include deionized water and a thickener.

[0013] Furthermore, the weight proportion of the deionized water in the anti-aging skin care composition is 65-90 parts, and the weight proportion of the thickener in the anti-aging skin care composition is 1-2 parts.

[0014] Furthermore, the thickener includes any one of carbomer, xanthan gum, and ammonium acryloyldimethyltaurate / VP copolymer.

[0015] The present invention also provides a preparation process for the anti-aging skin care composition as described herein. When the anti-aging skin care composition consists only of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, the raw materials only need to be mixed according to the formula; when the anti-aging skin care composition also contains auxiliary materials and preservatives, the preparation process includes the following steps: weighing the raw materials according to the formula, adding algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate to deionized water in sequence, stirring to dissolve, then adding a thickener and stirring evenly, and finally adding a preservative and stirring evenly to obtain the anti-aging skin care composition.

[0016] The present invention also provides the use of the anti-aging skin care composition described herein in the preparation of anti-aging cosmetics.

[0017] The present invention also provides an anti-aging cosmetic comprising the anti-aging skin care composition as described herein.

[0018] Furthermore, the anti-aging cosmetics include but are not limited to essences, creams, masks, body lotions, etc.

[0019] Advantageous Effects of the Invention

[0020] The present invention has found that a composition of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate can promote the synthesis of type I, III and IV collagen in fibroblasts. The three can synergistically promote the synthesis of type I, III and IV collagen to a significant extent, thereby having the effects of delaying aging, anti-wrinkle and firming, and can therefore be used in anti-aging products. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Shows the results of testing the effects of a single ingredient on type I collagen production.

[0022] Figure 2 Shows the results of testing the effects of single raw materials on type III collagen production.

[0023] Figure 3 Shows the results of testing the effects of single raw materials on type IV collagen production.

[0024] Figure 4 Shown are the results of testing the effects of single ingredients and ingredients in combination in varying amounts on type I collagen production.

[0025] Figure 5 Shown are the results of testing the effects of single ingredients and ingredients in combination in varying amounts on type I collagen production.

[0026] Figure 6 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type III collagen production.

[0027] Figure 7 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type III collagen production.

[0028] Figure 8 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type IV collagen production.

[0029] Figure 9 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type IV collagen production.

[0030] Figure 10 The average value of the average width of the outer corner wrinkles at various time points before and after the use of the sample tested in Example 2 is shown.

[0031] Figure 11 The graph shows the change rate of the average width of the outer canthus wrinkles at various time points before and after the use of the sample tested in Example 2.

[0032] Figure 12 The average value of the average width of under-eye wrinkles at various time points before and after use of the sample as tested in Example 2 is shown.

[0033] Figure 13 The graph shows the change rate of the average width of under-eye wrinkles at various time points before and after use of the sample as tested in Example 2.

[0034] Figure 14 Shown are illustrations of skin surface morphology analysis of the subjects tested in Example 2 before and after use of the samples.

[0035] Figure 15 The results show the effects of using a combination of raw materials other than those described in the present invention on the production of type I collagen. DETAILED DESCRIPTION

[0036] The present invention is further described below with reference to specific examples, which, however, are not intended to limit the present invention in any way. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in the art.

[0037] The algal oligosaccharides used were purchased from Qingdao Haida Marine Oligosaccharide Technology Co., Ltd., item number 84775-78-0, and were marine oligosaccharides extracted from algae (Giant algae) in the Antarctic waters of Chile; carboxymethyl deacetylated chitosan was purchased from Shanghai Chunlu Biochemistry Co., Ltd.; and hydrolyzed sodium hyaluronate was purchased from Shandong Guantianxia Biotechnology Co., Ltd.

[0038] Example 1: Effects of co-incubation of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate on the production of human type I collagen (Col I), human type III collagen (Col III) and human type IV collagen (Col IV) in human fibroblasts

[0039] Human skin fibroblasts were inoculated into MEM culture medium containing 5% fetal bovine serum by volume, cultured in a 5% CO2, 37°C incubator, and the medium was changed once every 3 days. After 7 days of culture, the original culture medium was aspirated, the cells were washed 3 times with PBS buffer, 0.5 mL of 0.25% Trypsin-1 mM EDTA4Na solution was added, and then 10 mL of MEM culture medium was added. The cells were suspended by pipetting, and then transferred to a centrifuge tube, centrifuged at 2000 rpm for 5 minutes, the supernatant was removed, and an appropriate amount of MEM culture medium containing 5% fetal bovine serum by volume was added. The cells were suspended by pipetting.

[0040] Take a 96-well plate and add 100 μL of cell suspension (containing 0.2×10 cells) to each well. 5), cultured in a 5% CO2, 37°C incubator for 24 h, the culture medium in the 96-well plate was discarded, and 100 μL of a culture medium containing a combination of one, two, or three of algal oligosaccharides, carboxymethyl chitosan, and hydrolyzed sodium hyaluronate was added, wherein the final mass concentrations of algal oligosaccharides, carboxymethyl chitosan, and hydrolyzed sodium hyaluronate in the culture medium were set as follows: two gradients of 0.06% and 0.1% for algal oligosaccharides, two gradients of 0.003% and 0.008% for carboxymethyl chitosan, and 0.05% for hyaluronic acid. In this way, culture media containing a single raw material, a combination of two raw materials, and a combination of three raw materials were respectively constructed for treating human skin fibroblasts; and an equal volume of blank culture medium was set as a blank control group. The 96-well plate was then placed in a 5% CO2, 37°C incubator for 24 h. The cell culture supernatant was collected and the content of type I, III, and IV collagen was determined by ELASA method, and the content increase rate was calculated (the blank control group was used as the basis for unified processing).

[0041] The results are as follows Figure 1-9 As shown. It can be seen that the content of collagen type I, III and IV in the single raw material showed an increasing trend compared with the blank group ( Figure 1-3 ), and when the three raw materials are combined in pairs, they show greater efficacy than a single raw material. In particular, when algal oligosaccharides and carboxymethyl chitosan are present at the same time, the content of type I, III, and IV collagen shows a significantly higher increase than that of a single raw material, showing a significant synergistic combination effect. When the three are added together, they show an even higher growth ratio ( Figure 4-9 ), in addition, whether in a combination of two or three, when controlling a single variable, when the amount of algae oligosaccharides and carboxymethyl chitosan added increased, the ratio increased, which shows that the efficacy of the combination increases with the increase in the amount of algae oligosaccharides and carboxymethyl chitosan added in the combination, showing a dose-dependent pattern.

[0042] The results of this example confirm that the combination of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate can significantly promote the synthesis of type I, III and IV collagen in human skin fibroblasts, thereby having the effects of delaying aging, anti-wrinkle and firming, and can therefore be used in anti-aging products.

[0043] Example 2:

[0044] An anti-aging skin care composition is prepared as follows: 10 parts by weight of algal oligosaccharides, 0.8 parts by weight of carboxymethyl chitosan, and 5 parts by weight of hydrolyzed sodium hyaluronate are sequentially added to 82.5 parts by weight of deionized water, stirred to dissolve, then 1.2 parts by weight of carbomer-940 is added and stirred evenly, and finally 0.5 parts by weight of caprylyl glycol is added and stirred evenly to obtain the anti-aging skin care composition.

[0045] An anti-aging facial cream is obtained by adding the anti-aging skin care composition into a facial cream matrix to prepare the facial cream, wherein the amount of the composition added to the facial cream is 1%.

[0046] The above-mentioned cream was tested for its anti-wrinkle efficacy with reference to T / ZHCA006-2019. In brief, 10 subjects (who met the outer canthus wrinkle rating of level 2 or above, and whose age range was 30-65 years old) were recruited, and the above-prepared cream was applied to the canthus and gently massaged until completely absorbed. The subjects used the test sample twice a day, once in the morning and once in the evening after cleansing, for 2 consecutive weeks. The average width of the outer canthus wrinkles and the average width of the under-eye wrinkles (Antera 3D) were analyzed before use, after 1 week of use, and after 2 weeks of use. The results of the average width analysis of the outer canthus wrinkles are shown in Tables 1 and Figure 10 、 11 As shown, it can be found that after using the sample for 2 weeks, the average width of the outer canthus wrinkles decreased by 39.23% compared with before use, and there was a significant difference (P < 0.05).

[0047] Table 1: Statistical results of the average width of outer corner wrinkles before and after using the samples (n=10)

[0048]

[0049] Note: ① Normality test P value>0.05, indicating that the data obeys the normal distribution, the paired t test is used. If it does not obey the normal distribution, the non-parametric rank sum test is used.

[0050] ② A significant P value of <0.05 indicates a significant difference compared to the pre-treatment value, indicated by "*"; a P value of <0.01 is indicated by "**"; a P value of <0.001 is indicated by "***". A significant P value ≥0.05 indicates no significant difference compared to the pre-treatment value, indicated by "-".

[0051] The results of the analysis of the average width of wrinkles under the eyes are shown in Table 2 and Figure 12 、 13 It can be seen that after using the sample for 2 weeks, the average width of under-eye wrinkles decreased by 39.86% compared with before use, and there was a significant difference (P < 0.05).

[0052] Table 2: Statistical results of average width of under-eye wrinkles before and after using the samples (n=10)

[0053]

[0054] Note: ① Normality test P value>0.05, indicating that the data obeys the normal distribution, the paired t test is used. If it does not obey the normal distribution, the non-parametric rank sum test is used.

[0055] ② A significant P value of <0.05 indicates a significant difference compared to the pre-treatment value, indicated by "*"; a P value of <0.01 is indicated by "**"; a P value of <0.001 is indicated by "***". A significant P value ≥0.05 indicates no significant difference compared to the pre-treatment value, indicated by "-".

[0056] in addition, Figure 14 This is an example of skin surface morphology analysis of a subject (Antera 3D). It can be seen that the subject's skin wrinkles have been significantly improved compared to before use.

[0057] Comparative Examples 1-3

[0058] The same method as in Example 1 was used to test the effects of 0.1% α-glucan oligosaccharide alone (Comparative Example 1), 0.1% α-glucan oligosaccharide + 0.008% carboxymethyl chitosan (Comparative Example 2), or 0.1% α-glucan oligosaccharide + 0.008% carboxymethyl chitosan + 0.05% hydrolyzed sodium hyaluronate (Comparative Example 3) on the production of type I collagen. The aforementioned concentrations are the final mass concentrations of the components in the culture medium for incubating cells. The results are shown in FIG. Figure 15 shown.

[0059] Comparative Examples 4-6

[0060] The same method as in Example 1 was used to test the effects of 0.1% chitosan oligosaccharide alone (Comparative Example 4), 0.1% chitosan oligosaccharide + 0.008% carboxymethyl chitosan (Comparative Example 5), or 0.1% chitosan oligosaccharide + 0.008% carboxymethyl chitosan + 0.05% hydrolyzed sodium hyaluronate (Comparative Example 6) on the production of type I collagen. The aforementioned concentrations are the final mass concentrations of the components in the culture medium for incubating cells. The results are shown in FIG. Figure 15 shown.

[0061] Comparative Examples 7-9

[0062] The same method as in Example 1 was used to test the effects of 0.1% trehalose alone (Comparative Example 7), 0.1% trehalose + 0.008% carboxymethyl chitosan (Comparative Example 8), or 0.1% trehalose + 0.008% carboxymethyl chitosan + 0.05% hydrolyzed sodium hyaluronate (Comparative Example 9) on the production of type I collagen. The aforementioned concentrations are the final mass concentrations of the components in the culture medium for incubating cells. The results are shown in FIG. Figure 15 shown.

[0063] from Figure 15The results showed that compared with the combination of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate of the present invention, the other three combinations had poor effects on promoting the production of type I, III and IV collagen, and did not show obvious synergistic effects.

[0064] It should be noted that the preferred embodiments of the present invention are given in the specification and drawings of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described in this specification. These embodiments are not intended to be additional limitations on the content of the present invention. The purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive. In addition, the above-mentioned technical features can be combined with each other to form various embodiments not listed above, which are all considered to be within the scope of the description of the present invention. Furthermore, it is obvious to those skilled in the art that improvements or changes can be made based on the above description, and all such improvements and changes should fall within the scope of protection of the claims attached to the present invention.

Claims

1. An application of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the preparation of an anti-aging skin care composition for enhancing type I collagen synthesis, characterized in that: The weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6-10 parts, 0.3-0.8 parts and 5 parts respectively; the algal oligosaccharides are marine oligosaccharides extracted from the algae Giant Algae in the Antarctic waters and Chilean waters.

2. An application of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the preparation of an anti-aging skin care composition for enhancing type IV collagen synthesis, characterized in that: The weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6-10 parts, 0.3-0.8 parts and 5 parts respectively; the algal oligosaccharides are marine oligosaccharides extracted from the algae Giant Algae in the Antarctic waters and Chilean waters.

3. The use according to any one of claims 1-2, characterized in that The anti-aging skin care composition further comprises a thickener, deionized water and a preservative.

4. The use according to claim 3, characterized in that The weight portion of the preservative in the anti-aging skin care composition is 0.3-0.6 parts.

5. The use according to claim 3, characterized in that The preservative includes any one of 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid, caprylyl glycol, pentylene glycol and ethylhexylglycerin.

6. The use according to claim 3, characterized in that The weight proportion of the deionized water in the anti-aging skin care composition is 65-90 parts, and the weight proportion of the thickener in the anti-aging skin care composition is 1-1.5 parts.

7. The use according to claim 3, characterized in that The thickener includes any one of carbomer, xanthan gum, and ammonium acryloyldimethyltaurate / VP copolymer.

8. The use according to claim 3, characterized in that The preparation process of the anti-aging skin care composition comprises the following steps: The raw materials are weighed according to the formula, and algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate are added to deionized water in sequence, stirred to dissolve, and then a thickener is added and stirred evenly, and finally a preservative is added and stirred evenly to obtain an anti-aging skin care composition.

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