Egg membrane peptide-containing material body with repairing effect and preparation method of egg membrane peptide-containing material body

Abstract

The invention relates to an egg membrane peptide-containing repairing efficacy material body and a preparation method thereof, and belongs to the technical field of dental oral care. According to the invention, the egg membrane peptide is used as a gingival protecting molecule, the hydroxyapatite is used as a carrier, and the sodium alginate is added in the preparation process to help the egg membrane peptide to be uniformly distributed on the surface of the hydroxyapatite, so that the repair and regeneration of enamel are realized, and the effect of protecting the gingiva is achieved. Meanwhile, the collagen-modified hydrated silica can maintain the mildness while being used as the abrasive, and meanwhile, the health state of gingiva and teeth is improved by utilizing the repairing function of collagen in the friction process. Through interaction of the raw materials, the prepared egg membrane peptide-containing repairing efficacy material body can achieve an excellent protection effect on tooth enamel and gingiva.

Description

Technical Field

[0001] The invention belongs to the technical field of dental oral care and relates to a repairing material containing egg membrane peptide and a preparation method thereof. Background Art

[0002] Tooth enamel is the hardest part of the tooth, but it can also be damaged due to age, bad eating habits (such as sweet foods, carbonated drinks), improper brushing habits, and technical operations such as orthodontics and teeth whitening. Once the tooth enamel is damaged, it can lead to problems such as tooth sensitivity and tooth decay. The gums are an important part of oral health, and gum problems such as gingivitis and periodontitis can also affect the overall health of the oral cavity.

[0003] Therefore, a repair product that can effectively achieve excellent protection of tooth enamel and gums is needed. Summary of the invention

[0004] The purpose of the present invention is to provide a repairing material containing egg membrane peptides and a preparation method thereof. The prepared repairing material containing egg membrane peptides can achieve excellent protection of tooth enamel and gums.

[0005] The purpose of the present invention can be achieved through the following technical solutions: A repairing material containing egg membrane peptide, the repairing material comprising the following raw materials in parts by weight: 0.3-0.5 parts of sodium benzoate, 0.6-1 parts of a conditioning agent, 10-15 parts of deionized water, 2-4 parts of a surfactant, 30-35 parts of a moisturizing agent, 20-30 parts of modified hydroxyapatite, 4-8 parts of a hydrated silica complex, 0.2-0.3 parts of saccharin sodium, 0.7-1 parts of a thickener, and 0.4-0.7 parts of a desensitizer, wherein the desensitizer is sodium monofluorophosphate; The modified hydroxyapatite comprises egg membrane peptide and hydroxyapatite, and the preparation process of the modified hydroxyapatite is as follows: Sodium alginate and deionized water were stirred at a speed of 250 r / min for 30 min in a mass ratio of (5-8):10 by a magnetic stirrer to obtain a sodium alginate solution after being evenly mixed, and then egg membrane peptide and hydroxyapatite were added in sequence, and the stirring speed was maintained for 30 min. After drying, the solution was ball-milled at a speed of 300 r / min for 3 h and then passed through a 200-mesh sieve to obtain the modified hydroxyapatite.

[0006] As a preferred technical solution of the present invention, the preparation process of the hydrated silica composite is as follows: S2.1, mix collagen with glacial acetic acid, and then heat at 80-90°C for 5-8h to obtain a collagen solution with a mass fraction of 15-20%; S2.2, mix hydrated silica and collagen solution, wherein the amount of hydrated silica and collagen solution is in the ratio of (5-10 g): (60-75 mL), oscillate ultrasonically until fully mixed, filter, vacuum dry, and then ball-mill at a speed of 300 r / min for 3 h, and then pass through a 200-mesh sieve to obtain the hydrated silica complex.

[0007] As a preferred technical solution of the present invention, the thickener is one or both of xanthan gum and cellulose gum.

[0008] As a preferred technical solution of the present invention, the moisturizing agent is one or both of sorbitol and polyethylene glycol-8.

[0009] As a preferred technical solution of the present invention, the regulator is one of dipotassium glycyrrhizinate, potassium chloride and sodium chloride.

[0010] As a preferred technical solution of the present invention, the surfactant is one of sodium lauryl sulfate and cocamidopropyl betaine.

[0011] As a preferred technical solution of the present invention, the mass ratio of the egg membrane peptide, the sodium alginate solution and the hydroxyapatite is (3-5): (50-60): (20-30).

[0012] As a preferred technical solution of the present invention, the drying temperature is 85° C. and the drying time is 12 hours.

[0013] As a preferred technical solution of the present invention, in step S2.2, the vacuum drying is carried out at a temperature of 80-95°C and for a time of 10-14 hours.

[0014] A method for preparing a repairing material containing egg membrane peptides, comprising the following preparation process: Sodium benzoate, a regulator and deionized water are mixed by weight, and stirred at a speed of 250 r / min for 80 to 100 min to obtain a mixture A; a surfactant, a humectant and a thickener are mixed and homogenized for 15 to 20 min to obtain a mixture B; modified hydroxyapatite, a hydrated silica complex, sodium saccharin and a desensitizer are mixed, and stirred at a speed of 300 r / min for 50 to 70 min to obtain a mixture C; mixtures A, B and C are evenly mixed, and stirred at a speed of 300 r / min for 60 to 90 min to obtain the repairing material containing the egg membrane peptide.

[0015] Egg membrane peptide is an active peptide substance extracted from egg membrane. It is rich in various amino acids and trace elements, such as collagen peptide, hyaluronic acid, chondroitin sulfate, etc. Egg membrane peptide can play an important role in gum protection toothpaste because of its excellent biological activity and repair ability. It helps to repair damaged gingival tissue and promote the regeneration and repair of gingival cells, thereby enhancing the health of the gums. Egg membrane peptide can also improve the oral environment, inhibit the growth of harmful bacteria, and reduce the occurrence of oral diseases. At the same time, it can also promote nutrient absorption and metabolism in the mouth and improve the overall health of the mouth.

[0016] Egg membrane peptide is a small molecule active peptide. The present invention mixes egg membrane peptide with hydroxyapatite. Hydroxyapatite is used as a carrier. Egg membrane peptide can be used as a gum protection component and carried by hydroxyapatite, which can achieve precise delivery and slow release. It can be more easily absorbed than amino acids or ordinary collagen, can be quickly absorbed by oral soft tissue, and repair oral mucosa and gums. At the same time, sodium alginate is added during the preparation process. Sodium alginate can absorb and chelate calcium ions in dental plaque and plaque, making the structure of dental plaque and plaque easy to fall off, so that users can easily remove the pigmentation on the surface of the teeth by brushing their teeth and restore the original color of the teeth.

[0017] Sodium alginate is a natural high molecular weight polysaccharide with good water solubility and dispersibility. In the process of loading egg membrane peptides on hydroxyapatite, sodium alginate can be used as a dispersant or surfactant to help the egg membrane peptides to be evenly distributed on the surface of hydroxyapatite, which can not only improve the loading efficiency, but also ensure the sustained release and biological activity of the egg membrane peptides in subsequent applications.

[0018] The modified hydroxyapatite has stronger adhesion properties and can efficiently play the role of hydroxyapatite as an induction factor for bones or teeth. It has good biocompatibility and high affinity with enamel, prevents the loss of calcium ions, seals dentinal tubules, solves problems such as enamel demineralization and dentinal tubule exposure allergy, realizes the repair and regeneration of enamel, and achieves the effect of gum protection.

[0019] The prepared collagen solution is mixed with hydrated silica to allow the collagen molecules to be adsorbed on the surface or pores of the hydrated silica. Collagen is an important component of gingival tissue and has the function of promoting gingival growth and repair. When collagen and hydrated silica are mixed, they can act together on the gums to enhance the health of the gums. In addition, hydrated silica, as an abrasive in toothpaste, has an excellent cleaning effect. When mixed with collagen, it can improve the cleaning effect on teeth and gums while maintaining mildness.

[0020] Beneficial effects of the present invention: The present invention utilizes egg membrane peptide as a gum protection molecule, uses hydroxyapatite as a carrier, and adds sodium alginate during the preparation process to help the egg membrane peptide be evenly distributed on the surface of hydroxyapatite, thereby achieving the repair and regeneration of tooth enamel and protecting the gums. At the same time, the hydrated silica modified by collagen can maintain mildness while being used as an abrasive, and the repair function of collagen is used during the friction process to improve the health of gums and teeth. The present invention uses the interaction of various raw materials to prepare a repairing material containing egg membrane peptide that can achieve excellent protection of tooth enamel and gums. DETAILED DESCRIPTION

[0021] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0022] In the following examples and comparative examples: egg membrane peptide was purchased from Ningxia Vanilla Biotechnology Co., Ltd.

[0023] Example 1 Preparation of modified hydroxyapatite: Sodium alginate and deionized water are stirred at a speed of 250 r / min for 30 minutes in a mass ratio of 5:10 by a magnetic stirrer to obtain a sodium alginate solution after uniform mixing, and then egg membrane peptide and hydroxyapatite are added in sequence, stirring is maintained at the speed for 30 minutes, and the mixture is dried at 85°C for 12 hours, ball milled at a speed of 300 r / min for 3 hours, and then passed through a 200-mesh sieve to obtain the modified hydroxyapatite. The mass ratio of the egg membrane peptide, sodium alginate solution and hydroxyapatite is 3:50:20.

[0024] Preparation of hydrated silica composite: S2.1, mixing collagen with glacial acetic acid, and then heating at 80°C for 5 hours to obtain a collagen solution with a mass fraction of 15%; S2.2. Mix hydrated silica and collagen solution, wherein the amount of hydrated silica and collagen solution is 5g:60mL, and mix them evenly by ultrasonic oscillation. Then, filter, vacuum dry, and ball mill at a speed of 300r / min for 3h, and then pass through a 200-mesh sieve to obtain the hydrated silica composite. The temperature during vacuum drying is 8°C and the time is 10h.

[0025] Preparation of the repairing material containing egg membrane peptides: According to weight proportions, 0.3 parts of sodium benzoate, 0.6 parts of dipotassium glycyrrhizinate and 10 parts of deionized water were mixed, and stirred at a speed of 250 r / min for 80 minutes to obtain a mixture A; 2 parts of sodium lauryl sulfate, 30 parts of sorbitol and 0.7 parts of xanthan gum were mixed and homogenized for 15 minutes to obtain a mixture B; 20 parts of modified hydroxyapatite, 4 parts of hydrated silica composite, 0.2 parts of sodium saccharin and 0.4 parts of sodium monofluorophosphate were mixed, and stirred at a speed of 300 r / min for 50 minutes to obtain a mixture C; mixtures A, B and C were evenly mixed, and stirred at a speed of 300 r / min for 60 minutes to obtain the repairing material containing egg membrane peptides.

[0026] Example 2 Preparation of modified hydroxyapatite: Sodium alginate and deionized water are stirred at a speed of 250 r / min for 30 minutes in a mass ratio of 6:10 by a magnetic stirrer to obtain a sodium alginate solution after uniform mixing, and then egg membrane peptide and hydroxyapatite are added in sequence, stirring is maintained at the speed for 30 minutes, and the mixture is dried at 85°C for 12 hours, ball milled at a speed of 300 r / min for 3 hours, and then passed through a 200-mesh sieve to obtain the modified hydroxyapatite. The mass ratio of the egg membrane peptide, sodium alginate solution and hydroxyapatite is 4:55:25.

[0027] Preparation of hydrated silica composite: S2.1, mixing collagen with glacial acetic acid, and then heating at 85°C for 6 hours to obtain a collagen solution with a mass fraction of 17%; S2.2. Mix hydrated silica and collagen solution, wherein the amount of hydrated silica and collagen solution is 7g:68mL, and mix them thoroughly and evenly by ultrasonic oscillation. Then, filter, vacuum dry, and ball mill at a speed of 300r / min for 3h, and then pass through a 200-mesh sieve to obtain the hydrated silica composite. The temperature during vacuum drying is 86°C and the time is 12h.

[0028] Preparation of the repairing material containing egg membrane peptides: According to weight proportions, 0.4 parts of sodium benzoate, 0.8 parts of dipotassium glycyrrhizinate and 13 parts of deionized water were mixed, and stirred at a speed of 250 r / min for 90 minutes to obtain a mixture A; 3 parts of sodium lauryl sulfate, 33 parts of sorbitol and 0.8 parts of xanthan gum were mixed and homogenized for 18 minutes to obtain a mixture B; 25 parts of modified hydroxyapatite, 6 parts of hydrated silica composite, 0.25 parts of sodium saccharin and 0.6 parts of sodium monofluorophosphate were mixed, and stirred at a speed of 300 r / min for 60 minutes to obtain a mixture C; mixtures A, B and C were evenly mixed, and stirred at a speed of 300 r / min for 70 minutes to obtain the repairing material containing egg membrane peptides.

[0029] Example 3 Preparation of modified hydroxyapatite: Sodium alginate and deionized water are stirred at a speed of 250 r / min for 30 minutes in a mass ratio of 8:10 by a magnetic stirrer to obtain a sodium alginate solution after uniform mixing, and then egg membrane peptide and hydroxyapatite are added in sequence, stirring is maintained at the speed for 30 minutes, and the mixture is dried at 85°C for 12 hours, ball milled at a speed of 300 r / min for 3 hours, and then passed through a 200-mesh sieve to obtain the modified hydroxyapatite. The mass ratio of the egg membrane peptide, sodium alginate solution and hydroxyapatite is 5:60:30.

[0030] Preparation of hydrated silica composite: S2.1, mixing collagen with glacial acetic acid, and then heating at 90°C for 8h to obtain a collagen solution with a mass fraction of 20%; S2.2. Mix hydrated silica and collagen solution, wherein the amount of hydrated silica and collagen solution is 10g:75mL, and mix them thoroughly and evenly by ultrasonic oscillation. Then, filter, vacuum dry, and ball mill at a speed of 300r / min for 3h, and then pass through a 200-mesh sieve to obtain the hydrated silica composite. The temperature during vacuum drying is 95°C and the time is 14h.

[0031] Preparation of the repairing material containing egg membrane peptides: According to weight proportions, 0.5 parts of sodium benzoate, 1 part of dipotassium glycyrrhizinate and 15 parts of deionized water were mixed, and stirred at a speed of 250 r / min for 100 minutes to obtain a mixture A; 4 parts of sodium lauryl sulfate, 35 parts of sorbitol and 1 part of xanthan gum were mixed and homogenized for 20 minutes to obtain a mixture B; 30 parts of modified hydroxyapatite, 8 parts of hydrated silica composite, 0.3 parts of sodium saccharin and 0.7 parts of sodium monofluorophosphate were mixed, and stirred at a speed of 300 r / min for 70 minutes to obtain a mixture C; mixtures A, B and C were evenly mixed, and stirred at a speed of 300 r / min for 90 minutes to obtain the repairing material containing egg membrane peptides.

[0032] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that no egg membrane peptide is added in Comparative Example 1, and the other operations are the same.

[0033] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the hydroxyapatite in Comparative Example 2 is not subjected to any modification treatment, and the other operations are the same.

[0034] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that the hydrated silica in Comparative Example 3 is not treated with collagen, and the other operations are the same.

[0035] Performance Test: A. Oral mucosal irritation test: 1. Test method: Appendix B.3 Oral mucosal irritation test in GB / T 16886.10-2017 Biological Evaluation of Medical Devices Part 10: Irritation and Skin Sensitization Test; 2. Testing environment: Ordinary environment animal room, Laboratory Animal Use License No.: SYXK (Guangdong) 2022-0198, temperature 18~26℃, relative humidity 30~70%; 3. Experimental animals and feed: 3 SPF-grade golden hamsters, male, weighing 100-104 g, the experimental animals were purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd., the experimental animal production license number is SCXK (Beijing) 2021-0011, the experimental animal quality certificate number is No.110011241108290461, and the feed was purchased from Guangdong Medical Experimental Animal Center, certificate number: No.2409060017; 4. Sample processing: The repairing material containing egg membrane peptide prepared in Example 1 was used directly; 5. Test methods: a) Administration process: Before administration, the cheek pouch was turned over and rinsed with saline. After checking for abnormalities, a cotton ball with a diameter of about 10 mm was dipped with 0.4 mL of the sample and placed in the right cheek pouch of the golden hamster. The time was recorded. The left cheek pouch was not treated and served as a control. After 5 minutes, the cotton ball was removed and the right cheek pouch was rinsed with saline. The cheek pouch was observed with the naked eye. The above steps were repeated every hour (±0.1 h) for a total of 4 hours, with 5 contacts; b) Animal observation: before each contact, check the cheek pouches, describe the general condition of the animal's cheek pouches, and determine the score of the animal's cheek pouch surface reaction during each observation period according to the scoring system given in Table B.2 of Part 10 of GB / T 16886.10-2017. Observe the cheek pouches with the naked eye (24±2) h after the last contact, euthanize the hamsters, remove the cheek pouches on both sides, fix them in 10% neutral formalin fixative, and then perform histological examination; Judging criteria: (1) Histopathological examination results The tissues were scored according to the scoring system given in Table B.3 of Part 10 of GB / T 16886.10-2017.

[0036] (2) The average score of the experimental group is obtained by adding up the average microscopic scores of all animals in the experimental group and dividing it by the total number of observations. The maximum score is 16. The same method is used for the control group. If the total score of the microscopic evaluation of the cheek pouch on the control side is greater than 9 points, it may indicate pathological changes, which may be caused by damage during the experimental operation, and a retest is required.

[0037] (3) The irritation index is obtained by subtracting the average score of the control group from the average score of the test group (see Table B.4 in Part 10 of GB / T 16886.10-2017).

[0038] The results are shown in Table 1: Table 1

[0039] According to the data in Table 1, the irritation index of the functional material prepared by the present invention on the oral mucosa of golden hamsters is 0, and the irritation reaction type is non-irritating.

[0040] B. Test on tooth enamel repair and remineralization effect: The repairing material containing egg membrane peptide prepared by the present invention can be directly used as toothpaste: According to the laboratory method (XYJC-SOP-CE-055 In vitro bovine tooth enamel demineralization-remineralization test), the samples were tested for their enamel repair and remineralization effects.

[0041] Blank control group: tooth grinding blocks before demineralization treatment; Negative control group: tooth grinding blocks after demineralization; Preparation of sample group slurry: Weigh a certain amount of the sample to be tested and deionized water in a ratio of 1:1.6 (sample: water), stir with a glass rod to disperse the toothpaste, and then stir with an automatic stirrer to form a homogenous slurry for use. The sample to be tested is the functional material prepared in the example and the comparative example.

[0042] Test method: (1) Preparation of tooth grinding blocks: The bovine tooth enamel was cut into 4mm*4mm*3mm specifications using a low-speed precision cutting machine, embedded in epoxy resin, and polished with a grinder and polisher for later use.

[0043] (2) Surface microhardness test before demineralization: Before demineralization of teeth, their surface microhardness was tested and the values ​​were recorded. Teeth with similar surface microhardness were selected for testing.

[0044] (3) Demineralization treatment and surface microhardness test: The tooth block was immersed in the demineralization solution and demineralized at a constant temperature of 37°C. After demineralization, its surface microhardness was tested and the values ​​were recorded.

[0045] (4) Remineralization treatment and surface microhardness test: Take an appropriate amount of sample and evenly apply it on the surface of demineralized bovine enamel. Place the teeth on a brush grinder for scrubbing twice a day for 2 minutes each time. During the interval, place the sample in 37°C artificial saliva and replace the artificial saliva every 24 hours. Select the remineralization treatment time as required and measure the surface microhardness after the remineralization is completed.

[0046] The demineralizing solution contained: 0.1 mol / L lactic acid, 0.1 mol / L sodium hydroxide, 30 g / L hydroxymethyl cellulose, and the pH was adjusted to 4.2 with 0.1 mol / L NaOH.

[0047] Artificial saliva: 0.78 g / L sodium dihydrogen phosphate, 0.795 g / L calcium chloride, 0.005 g / L sodium sulfide, 0.4 g / L potassium chloride, 0.4 g / L sodium chloride, 1 g / L urea, dissolved in 800 mL of deionized water, pH adjusted to 6.8, and volume adjusted to 1000 mL.

[0048] The test results are shown in the following table:

[0049] On the basis of meeting the effectiveness of the test conditions, compared with the negative control group, the microhardness value of the enamel surface of the embodiment group after 28 days of remineralization was significantly improved, with the improvement rates being 27.73%, 29.92% and 29.03%, respectively, and there was a significant difference in the surface microhardness between the two groups (P < 0.05), indicating that the sample has the effect of repairing enamel and remineralization. Therefore, the repair material prepared by the present invention has the effect of protecting enamel and protecting gums.

[0050] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A repairing material containing egg membrane peptide, characterized in that: The repairing material comprises the following raw materials in parts by weight: 0.3-0.5 parts of sodium benzoate, 0.6-1 parts of a conditioning agent, 10-15 parts of deionized water, 2-4 parts of a surfactant, 30-35 parts of a moisturizing agent, 20-30 parts of modified hydroxyapatite, 4-8 parts of a hydrated silica complex, 0.2-0.3 parts of saccharin sodium, 0.7-1 parts of a thickener, and 0.4-0.7 parts of a desensitizer, wherein the desensitizer is sodium monofluorophosphate; The modified hydroxyapatite comprises egg membrane peptide and hydroxyapatite, and the preparation process of the modified hydroxyapatite is as follows: Sodium alginate and deionized water were stirred at a speed of 250 r / min for 30 min in a mass ratio of (5-8):10 by a magnetic stirrer to obtain a sodium alginate solution after being evenly mixed, and then egg membrane peptide and hydroxyapatite were added in sequence, and the stirring speed was maintained for 30 min. After drying, the solution was ball-milled at a speed of 300 r / min for 3 h and then passed through a 200-mesh sieve to obtain the modified hydroxyapatite.

2. The repairing material containing egg membrane peptide according to claim 1, characterized in that: The preparation process of the hydrated silica composite is as follows: S2.1, mix collagen with glacial acetic acid, and then heat at 80-90°C for 5-8h to obtain a collagen solution with a mass fraction of 15-20%; S2.2, mix hydrated silica and collagen solution, wherein the amount of hydrated silica and collagen solution is in the ratio of (5-10 g): (60-75 mL), oscillate ultrasonically until fully mixed, filter, vacuum dry, and then ball-mill at a speed of 300 r / min for 3 h, and then pass through a 200-mesh sieve to obtain the hydrated silica complex.

3. The repairing material containing egg membrane peptide according to claim 1, characterized in that: The thickener is one or both of xanthan gum and cellulose gum.

4. The repairing material containing egg membrane peptide according to claim 1, characterized in that: The moisturizing agent is one or both of sorbitol and polyethylene glycol-8.

5. The repairing material containing egg membrane peptide according to claim 1, characterized in that: The regulator is one of dipotassium glycyrrhizinate, potassium chloride and sodium chloride.

6. The repairing material containing egg membrane peptide according to claim 1, characterized in that: The surfactant is one of sodium lauryl sulfate and cocamidopropyl betaine.

7. The repairing material containing egg membrane peptide according to claim 1, characterized in that: The mass ratio of the egg membrane peptide, sodium alginate solution and hydroxyapatite is (3-5): (50-60): (20-30).

8. The repairing material containing egg membrane peptide according to claim 1, characterized in that: The drying temperature is 85° C. and the drying time is 12 hours.

9. The repairing material containing egg membrane peptide according to claim 1, characterized in that: In step S2.2, the vacuum drying is carried out at a temperature of 80-95°C and for a time of 10-14 hours.

10. A method for preparing a repairing material containing egg membrane peptide according to any one of claims 1 to 9, characterized in that: The preparation process includes the following: Sodium benzoate, a conditioning agent and deionized water were mixed according to weight proportions, and stirred at a speed of 250 r / min for 80 to 100 min to obtain a mixture A; a surfactant, a humectant and a thickener were mixed and homogenized for 15 to 20 min to obtain a mixture B; The modified hydroxyapatite, hydrated silica complex, sodium saccharin and desensitizer are mixed, and stirred at a speed of 300 r / min for 50 to 70 minutes to obtain a mixture C; the mixtures A, B and C are mixed evenly, and stirred at a speed of 300 r / min for 60 to 90 minutes to obtain the repairing material containing the egg membrane peptide.

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