A spraying slurry ore source biological organic fertilizer and its preparation method

Abstract

The present invention discloses a sprayed bio-organic fertilizer from mineral sources and its preparation method, belonging to the technical field of bio-organic fertilizers. The fertilizer comprises the following raw materials in parts by weight: 70-80 parts of compound humic acid slurry, 0.1-0.5 part of trace elements, 10-20 parts of modified starch, and 0.1-0.5 part of microbial bacterial liquid. In the present invention, a Bacillus subtilis strain with high efficiency in converting lignite into humic acid is selected, and at the same time, Pseudomonas yamanorum and Bacillus thuringiensis are compounded. The three are fermented together to effectively increase the content of free humic acid. Finally, a spraying process is adopted, which can reduce the moisture of the organic fertilizer and improve the particle strength, and is suitable for the technology of simultaneous sowing of seeds and fertilizers. The organic fertilizer of the present invention is rich in nutrient elements and has good water solubility of humic acid, and has more advantages as a nutrient source for crops.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological organic fertilizers, and particularly relates to a spray-granulated mineral source biological organic fertilizer and a preparation method thereof. Background Art

[0002] Ordinary organic fertilizers are mainly fermented from agricultural wastes, livestock and poultry manure, etc. They have high heavy metal content, are contaminated with germs and eggs. At the same time, due to different sources of organic fertilizer raw materials, the nutrient variation is large, the product indexes are unstable, and problems such as seedling burning and secondary fermentation are likely to occur.

[0003] Humic acid is a natural and environmentally friendly organic functional additive for fertilizers, and plays an important role in improving soil quality, promoting plant growth and enhancing fertilizer efficiency. Humic acid is a weak acid substance, widely extracted from peat, lignite, weathered coal and plant and animal residue products. The common method is "alkali extraction and acid precipitation", and its solubility in water depends on the pH value and the concentration of metal ions. Humic acid is a complex high-molecular organic acid, usually containing a large number of aromatic structures and various functional groups, such as carboxyl groups, phenolic hydroxyl groups, ketone groups, aldehyde groups, etc. These functional groups endow humic acid with good chemical activity and complex chemical properties, and also help to chelate trace nutrient elements, improve the solubility of nutrients and the absorption and utilization rate of plants. The special physical and chemical properties of humic acid have led to its wide application in the fertilizer field, and it can effectively adapt to a variety of modern fertilizer irrigation technologies, and can take into account multiple scenarios such as chemical fertilizer combination, soil improvement, foliar spraying, drip irrigation and sprinkler irrigation. Humic acid fertilizers are mainly prepared by physically mixing humic acid with various nutrient element fertilizers. The simultaneous use of humic acid and chemical fertilizers can not only improve the utilization rate of nutrient elements by crops, but also reduce the application amount of chemical fertilizers and reduce the occurrence of soil pollution problems.

[0004] At present, the commonly used humic acid products in the market are alkali-extracted mineral source humic acid. Due to its high pH, it is easy to cause seedling burning during application; the mineral source humic acid products sold in the market mainly include potassium humate, potassium fulvate, humic acid water-soluble fertilizer, humic acid urea, humic acid compound fertilizer, etc. They mainly use coal humic acid, so they are also called mineral source humic acid. Biological humic acid refers to humic acid products prepared from biomass by using biotechnology (mainly fermentation method). Its composition is extremely complex. In addition to rich humic acid components such as fulvic acid, it also contains amino acids, enzymes, beneficial microorganisms, vitamins, trace elements, etc. It has the functions of promoting plant growth, enhancing plant stress resistance, improving soil and improving product quality. Biological humic acid has a small molecular weight, many functional groups and strong biological activity, and has good agricultural application effects.

[0005] However, most of the current humic acid fertilizers use a single humic acid product. The cost of using mineral source humic acid is high and its water solubility is poor. For existing biological humic acid products, the active ingredient content is not high, the fertilizer efficiency is unstable, and the use cost is high. For example, Chinese Patent Application CN201410571104.8 discloses an improved humic acid fertilizer and its processing technology. The fertilizer includes the following materials in parts by mass: 45% of mineral source humic acid, 20% of biochemical potassium humate, 1% of biological active enzyme, 7% of trace elements, 10% of urea, 8% of amino acids, 4% of potassium nitrate, 4% of potassium dihydrogen phosphate, and in addition, a certain amount of chelate added later. Therefore, how to improve the utilization rate and use effect of humic acid is a technical problem that needs to be solved urgently at present. Summary of the Invention

[0006] In view of the problems existing in the prior art, the present invention provides a humic acid organic fertilizer with diversified microorganisms, and combines a spraying process to effectively improve the application effect of humic acid and can play a role in regulating soil activity at the same time.

[0007] To achieve the above technical objectives, the technical solutions adopted by the present invention are as follows:

[0008] A spraying mineral source bio-organic fertilizer includes the following raw materials in parts by weight: 70 - 80 parts of composite humic acid slurry, 0.1 - 0.5 parts of trace elements, 10 - 20 parts of modified starch, and 0.1 - 0.5 parts of microbial bacterial liquid.

[0009] Further, the trace elements are one or more of boric acid, zinc sulfate, ferrous sulfate, and manganese sulfate.

[0010] Further, the microbial bacterial liquid is one or more of Bacillus licheniformis, Trichoderma harzianum, and Paenibacillus megaterium. The microbial bacterial liquid is obtained by mixing bacterial powder and molasses in a mass ratio of 2:1 and dispersing them in water to obtain a microbial bacterial liquid with an effective viable count ≥ 200 million / mL.

[0011] Further, the composite humic acid slurry includes a mineral source humic acid slurry and a biological humic acid slurry, and its preparation method is as follows:

[0012] (1) Preparation of mineral source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH = 9 - 10, stir at a constant temperature of 90 - 100 °C for 1 - 3 h to obtain solution A; add 4% of the mass of formaldehyde and 8% of the mass of sodium sulfite of humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 - 3 h, and after sterilization, the mineral source humic acid slurry is obtained;

[0013] (2) Preparation of biological humic acid slurry: First, lignite is crushed and screened using a ball mill to obtain a sample with a particle size of 0.125 - 0.250 mm. Then, the sample is soaked and oxidized with 10 mol / L nitric acid for 48 h, washed with deionized water until neutral, dried, and sterilized to obtain a pretreated coal sample. Take 80 g of the pretreated coal sample, 10 g of mineral source humic acid slurry, add 10 mL of compound fermentation bacterial liquid and 30 g of nutrient material, mix evenly, adjust the moisture content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After fermentation, a compound humic acid slurry is obtained.

[0014] Furthermore, the nutrient material is obtained by mixing sucrose and yeast powder in a mass ratio of 3:1.

[0015] Furthermore, the compound fermentation bacterial liquid in step (2) is composed of Pseudomonas yamanensis ( Pseudomonas yamanorum ), Bacillus subtilis ( Bacillus subtilis ), and Bacillus thuringiensis ( Bacillus thuringiensis ), and the volume ratio of the three is 1:1:1. The specific preparation method is as follows: The cryopreserved Pseudomonas yamanensis, Bacillus subtilis, and Bacillus thuringiensis are respectively inoculated onto LB solid medium by the plate streaking method and cultured at a suitable temperature of 25 - 30 °C for 48 h. Then, one loop of colony is respectively inoculated into LB liquid medium, and in a constant temperature shaker, under the conditions of 25 - 30 °C and 120 r / min, shake-cultured for 48 h to obtain three kinds of bacterial liquids; the three kinds of bacterial liquids are mixed evenly according to a volume ratio of 1:1:1, and then the mixed bacterial liquid is inoculated into 1 L of LB liquid medium at an inoculation amount of 5% and enlarged cultured in a constant temperature shaker at 25 - 30 °C for 96 h to obtain the compound fermentation bacterial liquid.

[0016] Furthermore, the strain number of Pseudomonas yamanensis is CGMCC No.1.16088, and the original preservation time is February 13, 2017; the strain number of Bacillus thuringiensis is CGMCC No.1.8790, and the original preservation time is October 21, 2008. Both Pseudomonas yamanensis and Bacillus thuringiensis are purchased from the China General Microbiological Culture Collection Center; Pseudomonas yamanensis and Bacillus thuringiensis can be queried and purchased through the public catalog of the preservation center, so there is no need for repeated biological preservation. The preservation number of Bacillus subtilis is CGMCC No.44482, which is preserved in the China General Microbiological Culture Collection Center, the preservation date is September 12, 2023, and the preservation address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0017] The Bacillus subtilis was isolated from oilfield waste sludge, and the isolation and purification method is as follows: After drying the sludge, it is placed in a sterilized container. 7 g of the soil sample is added to a 250 mL Erlenmeyer flask containing 90 mL of sterile water, and several glass beads are added. It is cultured at 28°C and 220 r / min for 2 h. 1.0 mL of the mixed solution is taken and placed in a test tube containing 9.0 mL of sterile water, and mixed evenly to prepare a bacterial suspension with concentration gradients of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 . 0.1 mL of each concentration of the bacterial suspension is taken and spread on the YG plate. Three replicates are made for each concentration and cultured at 28 - 30°C for 2 - 3 d. Single colonies with different morphologies are picked and streaked and purified on the YG plate. The obtained single colonies are inoculated on the LB slant and cultured at 2°C for 2 d and stored at 4°C.

[0018] The composition of the YG plate (g / L) is as follows: yeast extract 1.00, glucose 1.00, K2HPO4 0.30, KH2PO4 0.25, MgSO4 0.20, pH 7.2 - 7.4 (for solid medium: add 2% agar).

[0019] Strain screening: For each strain, a 250 mL conical flask is used, and 150 mL of LB liquid medium is added and sterilized in a high-pressure steam sterilizer at 121°C for 20 min. The strain is inoculated into the corresponding liquid medium in the Erlenmeyer flask. After 3 days of culture, 5 g of lignite coal sample is added to each Erlenmeyer flask at the same time. After mixing evenly, the conical flask is placed in a constant temperature shaker and oscillated and cultured for 10 d. The treated lignite samples in the culture medium are collected, and their humic acid content is measured. The strain with the best effect of converting lignite to humic acid is screened out as the target strain Bacillus subtilis.

[0020] The obtained Bacillus subtilis is cultured on the LB solid medium. The colony edge is irregular, showing irregular dots, the surface is wrinkled and rough, and it is light yellow. The colony diagram is as Figure 1 shown.

[0021] A preparation method of a spraying mineral source biological organic fertilizer includes the following steps:

[0022] (1) According to weight parts, the composite humic acid slurry, trace elements, and modified starch are mixed and stirred evenly to obtain a spraying slurry, and the slurry is transported to a granulator for spraying granulation;

[0023] (2) Screening: The particles are screened through a rotary screen, and the particles with a particle size of 2 mm - 4 mm are selected as the finished particles, and those less than 2 mm or greater than 4 mm are sent back for reprocessing;

[0024] (3) Cooling: The dried granules are cooled by rolling in a cooling drum, and the cooling time is 10 min - 30 min;

[0025] (4) Bacterium addition: After cooling, the granules enter the drum, and the microbial bacterial liquid is atomized and evenly coated on the surface of the granules;

[0026] (5) Packaging and warehousing: The obtained granules are inspected, packaged and warehoused to obtain the finished product of biological organic fertilizer.

[0027] Further, in the spray granulation of step (1), the head temperature is 400 - 450 °C, the tail temperature is 60 - 65 °C, and the spray volume is 10 - 15 m 3 / h.

[0028] Beneficial effects:

[0029] (1) First, the present invention pre-treats the commercial humic acid raw material to obtain a mineral source humic acid slurry, which increases its water solubility to a certain extent. Subsequently, lignite raw material is added and fermented with the mineral source humic acid slurry by microorganisms.

[0030] (2) A strain of Bacillus subtilis with high efficiency in converting lignite to produce humic acid is screened, and at the same time, Pseudomonas yamanorum and Bacillus thuringiensis are compounded. The three ferment together, effectively increasing the content of free humic acid. At the same time, a large amount of enzymes are produced during the fermentation process. These enzymes can catalyze and degrade the macromolecular humic acid into small molecules, thereby improving the activity of humic acid. The finally obtained humic acid product has a high active ingredient and can be effectively absorbed by crops, promoting crop growth.

[0031] (3) The present invention wraps microorganisms on the outer layer and adds modified starch and trace elements at the same time, which can promote the growth and reproduction of microorganisms. The trace elements provide supplementary nutrition, and the wrapping of microorganisms can effectively increase the microbial biomass and enzyme activity in the soil, thereby achieving the effect of promoting nutrient cycling.

[0032] (4) Finally, the present invention adopts a spray granulation process. The spray granulation technology can produce high-quality fertilizer granules. These granules have a high nutrient content and good physical properties. At the same time, spray granulation can reduce the moisture content of the organic fertilizer and improve the granule strength, which is suitable for the technology of simultaneous sowing of seeds and fertilizers. The organic fertilizer of the present invention is rich in nutrient elements and has good water solubility of humic acid, and has more advantages as a nutrient source for crops. Description of the drawings

[0033] Figure 1 It is the colony map of Bacillus subtilis of the present invention;

[0034] Figure 2This is the coal-dissolving effect diagram of Pseudomonas yamanensis, Bacillus subtilis and Bacillus thuringiensis of the present invention. Among them, (a) is Pseudomonas yamanensis, (b) is Bacillus subtilis, and (c) is Bacillus thuringiensis. Detailed implementation mode

[0035] The technical solution of the present invention will be further described below in conjunction with specific embodiments, but not limited thereto.

[0036] Example 1

[0037] A spraying bio-organic fertilizer from ore source comprises the following raw materials in parts by weight: 70 parts of compound humic acid slurry, 0.4 part of trace elements, 20 parts of modified starch, and 0.3 part of microbial bacterial liquid.

[0038] The trace elements are obtained by mixing boric acid and zinc sulfate in a mass ratio of 1:1.

[0039] The microbial bacterial liquid is Bacillus licheniformis. The microbial bacterial liquid is obtained by mixing bacterial powder and molasses in a mass ratio of 2:1 and then dispersing them in water to obtain a microbial bacterial liquid with an effective viable bacteria count ≥ 200 million / mL.

[0040] The compound humic acid slurry comprises ore-source humic acid slurry and biological humic acid slurry, and its preparation method is as follows:

[0041] (1) Preparation of ore-source humic acid slurry: Dissolve 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9-10, and stir at a constant temperature of 90-100 °C for 1 h to obtain solution A; add 4% of the mass of formaldehyde of humic acid and 8% of the mass of sodium sulfite of humic acid to solution A, stir at 90-100 °C and 0.1 MPa for 1 h, and after sterilization, the ore-source humic acid slurry is obtained;

[0042] (2) Preparation of biological humic acid slurry: First, use a ball mill to crush lignite and screen it to obtain a sample with a particle size of 0.125-0.250 mm, then soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; take 80 g of the pretreated coal sample, 10 g of the ore-source humic acid slurry, add 10 mL of compound fermentation bacterial liquid and 30 g of nutrient material, mix them evenly, adjust the water content to 60-70%, and carry out mixed fermentation at 26-30 °C for 10 d. After the fermentation is completed, the compound humic acid slurry is obtained.

[0043] The nutrient material is obtained by mixing sucrose and yeast powder in a mass ratio of 3:1.

[0044] The compound fermentation bacterial liquid in step (2) is composed of Pseudomonas yamanensis ( Pseudomonas yamanorum ), Bacillus subtilis ( Bacillus subtilis ), and Bacillus thuringiensis (Bacillus thuringiensis It consists of (), and the volume ratio of the three is 1:1:1. The specific preparation method is as follows: The Pseudomonas yamanensis, Bacillus subtilis, and Bacillus thuringiensis stored frozen are respectively inoculated onto LB solid medium by the streak plate method and cultured at a suitable temperature of 25 - 30 °C for 48 h. Then, one loop of each colony is respectively inoculated into LB liquid medium and cultured in a constant temperature shaker at 25 - 30 °C and 120 r / min for 48 h to obtain three kinds of bacterial solutions; the three kinds of bacterial solutions are mixed evenly according to the volume ratio of 1:1:1, and then the mixed bacterial solution is inoculated into 1 L of LB liquid medium at an inoculation amount of 5% and expanded cultured in a constant temperature shaker at 25 - 30 °C for 96 h to obtain a composite fermentation bacterial solution.

[0045] The strain number of the Pseudomonas yamanensis is CGMCC No.1.16088, and the strain number of the Bacillus thuringiensis is CGMCC No.1.8790. Both are purchased from the China General Microbiological Culture Collection Center; the Pseudomonas yamanensis and Bacillus thuringiensis can be queried and purchased through the public catalog of the preservation center, so there is no need for repeated biological preservation. The preservation number of the Bacillus subtilis is CGMCC No.44482, which is preserved in the China General Microbiological Culture Collection Center. The preservation date is September 12, 2023, and the preservation address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0046] The Bacillus subtilis is isolated from oilfield waste sludge. The isolation and purification method is as follows: After drying the sludge, it is placed in a sterilized container. 7 g of the soil sample is added to a 250 mL Erlenmeyer flask containing 90 mL of sterile water, and several glass beads are added. It is cultured at 28 °C and 220 r / min for 2 h. 1.0 mL of the mixed solution is taken and placed in a test tube containing 9.0 mL of sterile water, and mixed evenly to prepare bacterial suspensions with concentration gradients of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 bacterial suspensions. 0.1 mL of each concentration of bacterial suspension is spread on a YG plate, and 3 replicates are made for each concentration. It is cultured at 28 - 30 °C for 2 - 3 d. Single colonies with different morphologies are picked and streaked and purified on the YG plate. The obtained single colonies are inoculated onto an LB slant and cultured at 2 °C for 2 d and stored at 4 °C.

[0047] The composition of the YG plate (g / L) is as follows: yeast extract 1.00, glucose 1.00, K2HPO4 0.30, KH2PO4 0.25, MgSO4 0.20, pH 7.2 - 7.4 (for solid medium: add 2% agar).

[0048] Strain screening: For each strain, a 250 mL conical flask was used, and 150 mL of LB liquid medium was added. It was sterilized in a high-pressure steam sterilizer at 121 °C for 20 min. The strain was inoculated into the corresponding liquid medium in an Erlenmeyer flask. After 3 days of cultivation, 5 g of lignite coal samples were added to each Erlenmeyer flask simultaneously. After mixing evenly, the conical flasks were placed in a constant temperature shaker and shaken for 10 days. The treated lignite samples in the medium were collected, and their humic acid content was measured. The strain with the best effect of converting lignite to humic acid was selected as the target strain Bacillus subtilis.

[0049] A preparation method of a spraying ore-source biological organic fertilizer includes the following steps:

[0050] (1) Mix the composite humic acid slurry, trace elements, and modified starch evenly by weight to obtain a spraying slurry. The slurry is transported to a granulator for spraying granulation;

[0051] (2) Screening: The particles are screened through a rotary screen, and the particles with a particle size of 2 mm - 4 mm are selected as finished particles, and those smaller than 2 mm or larger than 4 mm are reworked;

[0052] (3) Cooling: The dried particles are cooled by rolling in a cooling drum, and the cooling time is 10 min - 30 min;

[0053] (4) Adding bacteria: After cooling, the particles enter the drum, and the microbial bacterial liquid is atomized and evenly coated on the surface of the particles;

[0054] (5) Packaging and warehousing: The obtained particles are inspected, packaged, and warehoused to obtain the finished biological organic fertilizer.

[0055] In the spraying granulation in step (1), the head temperature is 400 °C, the tail temperature is 60 - 65 °C, and the spraying amount is 10 m 3 / h.

[0056] The product performance is detected according to the corresponding method in the national quality standard NY525 - 2021 for organic fertilizers. The organic matter of the product is ≥ 60%, the moisture is ≤ 10%, the pH is 6 - 9, and the effective viable bacteria count is ≥ 100 million / g.

[0057] Example 2

[0058] A spraying ore-source biological organic fertilizer includes the following raw materials by weight: 75 parts of composite humic acid slurry, 0.1 part of trace elements, 15 parts of modified starch, and 0.1 part of microbial bacterial liquid.

[0059] The trace elements are obtained by mixing ferrous sulfate and manganese sulfate in a mass ratio of 2:1.

[0060] The microbial liquid is Paenibacillus megaterium. The microbial liquid is obtained by mixing bacterial powder and molasses according to a mass ratio of 2:1, and dispersing them in water to obtain a microbial liquid with an effective viable count of ≥ 200 million / mL.

[0061] The composite humic acid slurry contains a mineral-source humic acid slurry and a bio-humic acid slurry, and its preparation method is as follows:

[0062] (1) Preparation of the mineral-source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9-10, and stir for 1 h under the condition of constant temperature at 90-100 °C to obtain solution A; add 4% of formaldehyde by mass of humic acid and 8% of sodium sulfite by mass of humic acid to solution A, stir at 90-100 °C and 0.1 MPa for 2 h, and after sterilization, the mineral-source humic acid slurry is obtained;

[0063] (2) Preparation of the bio-humic acid slurry: First, use a ball mill to crush and screen lignite to obtain a sample with a particle size of 0.125-0.250 mm, then soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; take 80 g of the pretreated coal sample, 10 g of the mineral-source humic acid slurry, add 10 mL of the composite fermentation bacterial liquid and 30 g of the nutrient material, mix them evenly, adjust the water content to 60-70%, and carry out mixed fermentation at 26-30 °C for 10 d. After the fermentation is completed, the composite humic acid slurry is obtained.

[0064] The nutrient material is obtained by mixing sucrose and yeast powder according to a mass ratio of 3:1.

[0065] The composite fermentation bacterial liquid in step (2) is composed of Pseudomonas yamana ( Pseudomonas yamanorum ), Bacillus subtilis ( Bacillus subtilis ), and Bacillus thuringiensis ( Bacillus thuringiensis ), and the volume ratio of the three is 1:1:1. The specific preparation method is as follows: Inoculate the cryopreserved Pseudomonas yamana, Bacillus subtilis, and Bacillus thuringiensis onto an LB solid medium by the plate streaking method respectively, culture them at a suitable temperature of 25-30 °C for 48 h, then take a loop of colonies and inoculate them into an LB liquid medium respectively, and oscillate and culture them in a constant-temperature shaker at 25-30 °C and 120 r / min for 48 h to obtain three bacterial liquids; mix the three bacterial liquids evenly according to a volume ratio of 1:1:1, then inoculate the mixed bacterial liquid into 1 L of LB liquid medium at an inoculation amount of 5%, and expand the culture in a constant-temperature shaker at 25-30 °C for 96 h to obtain the composite fermentation bacterial liquid.

[0066] The strain number of the Pseudomonas yamanensis is CGMCC No. 1.16088, and the strain number of the Bacillus thuringiensis is CGMCC No. 1.8790. Both are purchased from the China General Microbiological Culture Collection Center; the Pseudomonas yamanensis and the Bacillus thuringiensis can be queried and purchased through the public catalog of the preservation center, so there is no need for repeated biological preservation. The preservation number of the Bacillus subtilis is CGMCC No. 44482, which is preserved in the China General Microbiological Culture Collection Center. The preservation date is September 12, 2023, and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0067] The isolation and screening method of the Bacillus subtilis is the same as that in Example 1.

[0068] A preparation method of a sprayed ore source biological organic fertilizer includes the following steps:

[0069] (1) Mix and stir evenly the composite humic acid slurry, trace elements, and modified starch by weight to obtain a sprayed slurry, and transport the slurry to a granulator for spray granulation;

[0070] (2) Screening: The particles are screened through a rotary screen, and the particles with a particle size of 2 mm - 4 mm are selected as finished particles, and those less than 2 mm or greater than 4 mm are sent back for reprocessing;

[0071] (3) Cooling: Roll the dried particles in a cooling drum for cooling treatment, and the cooling time is 10 min - 30 min;

[0072] (4) Adding bacteria: After cooling, the particles enter the drum, and the microbial bacterial liquid is atomized and evenly coated on the surface of the particles;

[0073] (5) Packaging and warehousing: The obtained particles are inspected, packaged and warehoused to obtain the finished biological organic fertilizer.

[0074] In the spray granulation in step (1), the head temperature is 400 °C, the tail temperature is 60 - 65 °C, and the spraying amount is 11 m 3 / h.

[0075] The product performance is detected according to the corresponding method in the national quality standard NY525 - 2021 for organic fertilizers. The organic matter of the product is ≥ 60%, the moisture is ≤ 10%, the pH is 6 - 9, and the effective viable bacteria count is ≥ 100 million / g.

[0076] Example 3

[0077] A sprayed ore source biological organic fertilizer includes the following raw materials by weight: 80 parts of composite humic acid slurry, 0.5 part of trace elements, 10 parts of modified starch, and 0.5 part of microbial bacterial liquid.

[0078] The trace elements are obtained by mixing boric acid, zinc sulfate, ferrous sulfate, and manganese sulfate in a mass ratio of 1:1:2:3.

[0079] The microbial bacterial liquid is Bacillus licheniformis. The microbial bacterial liquid is obtained by mixing bacterial powder and molasses in a mass ratio of 2:1, and then dispersing them in water to obtain a microbial bacterial liquid with an effective viable bacteria count ≥ 200 million / mL.

[0080] The composite humic acid slurry contains a mineral source humic acid slurry and a biological humic acid slurry, and its preparation method is as follows:

[0081] (1) Preparation of the mineral source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, and stir for 3 h under the condition of constant temperature at 90 - 100 °C to obtain solution A; add 4% of the mass of formaldehyde and 8% of the mass of sodium sulfite of humic acid to solution A, stir for 3 h at 90 - 100 °C and 0.1 MPa, and after sterilization, the mineral source humic acid slurry is obtained;

[0082] (2) Preparation of the biological humic acid slurry: First, crush lignite with a ball mill and screen it to obtain a sample with a particle size of 0.125 - 0.250 mm, then soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; take 80 g of the pretreated coal sample, 10 g of the mineral source humic acid slurry, add 10 mL of the composite fermentation bacterial liquid and 30 g of the nutrient material, mix them evenly, adjust the moisture content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation is completed, the composite humic acid slurry is obtained.

[0083] The nutrient material is obtained by mixing sucrose and yeast powder in a mass ratio of 3:1.

[0084] In step (2), the composite fermentation bacterial liquid is composed of Pseudomonas yamana ( Pseudomonas yamanorum ), Bacillus subtilis ( Bacillus subtilis ), and Bacillus thuringiensis ( Bacillus thuringiensis ), and the volume ratio of the three is 1:1:1. The specific preparation method is as follows: Inoculate the cryopreserved Pseudomonas yamana, Bacillus subtilis, and Bacillus thuringiensis onto an LB solid medium by the plate streaking method respectively, culture them at a suitable temperature of 25 - 30 °C for 48 h, then take one loop of the colonies and inoculate them into an LB liquid medium respectively, and in a constant temperature shaker, under the conditions of 25 - 30 °C and 120 r / min, oscillate and culture for 48 h to obtain three kinds of bacterial liquids; mix the three kinds of bacterial liquids evenly according to a volume ratio of 1:1:1, and then inoculate the mixed bacterial liquid into 1 L of LB liquid medium at an inoculation amount of 5%, and expand the culture in a constant temperature shaker at 25 - 30 °C for 96 h to obtain the composite fermentation bacterial liquid.

[0085] The strain number of the Pseudomonas yamanensis is CGMCC No. 1.16088, and the strain number of the Bacillus thuringiensis is CGMCC No. 1.8790. Both are purchased from the China General Microbiological Culture Collection Center; the Pseudomonas yamanensis and the Bacillus thuringiensis can be queried and purchased through the public catalog of the preservation center, so there is no need for repeated biological preservation. The preservation number of the Bacillus subtilis is CGMCC No. 44482, which is preserved in the China General Microbiological Culture Collection Center. The preservation date is September 12, 2023, and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0086] The isolation and screening method of the Bacillus subtilis is the same as that in Example 1.

[0087] A preparation method of a spray granulation mineral source biological organic fertilizer includes the following steps:

[0088] (1) Mix the composite humic acid slurry, trace elements, and modified starch evenly by weight to obtain a spray granulation slurry, and transport the slurry to a granulator for spray granulation;

[0089] (2) Screening: The particles are screened through a rotary screen, and the particles with a particle size of 2 mm - 4 mm are selected as finished particles, and those less than 2 mm or greater than 4 mm are reworked;

[0090] (3) Cooling: Roll the dried particles in a cooling drum for cooling treatment, and the cooling time is 10 min - 30 min;

[0091] (4) Adding bacteria: After cooling, the particles enter the drum, and the microbial bacterial liquid is atomized and evenly coated on the surface of the particles;

[0092] (5) Packaging and warehousing: The obtained particles are inspected, packaged and warehoused to obtain the finished biological organic fertilizer.

[0093] In the spray granulation in step (1), the head temperature is 450 °C, the tail temperature is 60 - 65 °C, and the spray volume is 15 m 3 / h.

[0094] The product performance is detected according to the corresponding method in the national quality standard NY525 - 2021 of organic fertilizer. The organic matter of the product is ≥ 60%, the moisture is ≤ 10%, the pH is 6 - 9, and the effective viable bacteria count is ≥ 100 million / g.

[0095] Example 4

[0096] A spray granulation mineral source biological organic fertilizer includes the following raw materials by weight: 72 parts of composite humic acid slurry, 0.3 part of trace elements, 15 parts of modified starch, and 0.4 part of microbial bacterial liquid.

[0097] The trace elements are obtained by mixing boric acid, zinc sulfate, and ferrous sulfate in a mass ratio of 1:2:1.

[0098] The microbial bacterial liquid is Bacillus licheniformis. The microbial bacterial liquid is obtained by mixing bacterial powder and molasses in a mass ratio of 2:1, and then dispersing them in water to obtain a microbial bacterial liquid with an effective viable bacteria count of ≥ 200 million / mL.

[0099] The composite humic acid slurry contains a mineral source humic acid slurry and a biological humic acid slurry, and its preparation method is as follows:

[0100] (1) Preparation of the mineral source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, stir for 1 h under the condition of constant temperature at 90 - 100 °C to obtain solution A; add formaldehyde with a mass of 4% of the humic acid and sodium sulfite with a mass of 8% of the humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 h, and after sterilization, the mineral source humic acid slurry is obtained;

[0101] (2) Preparation of the biological humic acid slurry: First, crush lignite with a ball mill and sieve it to obtain a sample with a particle size of 0.125 - 0.250 mm. Then, soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; take 80 g of the pretreated coal sample, 10 g of the mineral source humic acid slurry, add 10 mL of the composite fermentation bacterial liquid and 30 g of the nutrient material, mix them evenly, adjust the moisture content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation is completed, the composite humic acid slurry is obtained.

[0102] The nutrient material is obtained by mixing sucrose and yeast powder in a mass ratio of 3:1.

[0103] In step (2), the composite fermentation bacterial liquid is composed of Pseudomonas yamana ( Pseudomonas yamanorum ), Bacillus subtilis ( Bacillus subtilis ), and Bacillus thuringiensis ( Bacillus thuringiensis ), and the volume ratio of the three is 1:1:1. The specific preparation method is as follows: Inoculate the cryopreserved Pseudomonas yamana, Bacillus subtilis, and Bacillus thuringiensis onto an LB solid medium by the plate streaking method respectively, culture them at a suitable temperature of 25 - 30 °C for 48 h, then take one loop of the colony and inoculate it into an LB liquid medium respectively, and shake culture at 25 - 30 °C and 120 r / min in a constant temperature shaker for 48 h to obtain three kinds of bacterial liquids; mix the three kinds of bacterial liquids evenly according to a volume ratio of 1:1:1, and then inoculate the mixed bacterial liquid into 1 L of LB liquid medium at an inoculation amount of 5%, and expand the culture in a constant temperature shaker at 25 - 30 °C for 96 h to obtain the composite fermentation bacterial liquid.

[0104] The strain number of the Pseudomonas yamanensis is CGMCC No. 1.16088, and the strain number of the Bacillus thuringiensis is CGMCC No. 1.8790. Both are purchased from the China General Microbiological Culture Collection Center; the Pseudomonas yamanensis and Bacillus thuringiensis can be queried and purchased through the public catalog of the collection center, so there is no need for repeated biological preservation. The preservation number of the Bacillus subtilis is CGMCC No. 44482, which is preserved in the China General Microbiological Culture Collection Center. The preservation date is September 12, 2023, and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0105] The isolation and screening method of the Bacillus subtilis is the same as that in Example 1.

[0106] A preparation method of a spraying ore-source biological organic fertilizer includes the following steps:

[0107] (1) Mix and stir evenly the composite humic acid slurry, trace elements, and modified starch by weight to obtain a spraying slurry, and transport the slurry to a granulator for spraying granulation;

[0108] (2) Screening: The particles are screened by a rotary screen, and the particles with a particle size of 2 mm - 4 mm are selected as the finished particles, and those smaller than 2 mm or larger than 4 mm are reworked;

[0109] (3) Cooling: Roll the dried particles in a cooling drum for cooling treatment, and the cooling time is 10 min - 30 min;

[0110] (4) Adding bacteria: After cooling, the particles enter the drum, and the microbial bacterial liquid is atomized and evenly coated on the surface of the particles;

[0111] (5) Packaging and warehousing: The obtained particles are inspected and packaged and warehoused to obtain the finished biological organic fertilizer.

[0112] In the spraying granulation in step (1), the head temperature is 450 °C, the tail temperature is 60 - 65 °C, and the spraying amount is 12 m 3 / h.

[0113] The product performance is detected according to the corresponding method in the national quality standard NY525 - 2021 of organic fertilizers. The organic matter of the product is ≥ 60%, the moisture is ≤ 10%, the pH is 6 - 9, and the effective viable bacteria count is ≥ 100 million / g.

[0114] Comparative Example 1

[0115] A spraying ore-source biological organic fertilizer includes the following raw materials by weight: 70 parts of ore-source humic acid slurry, 0.4 parts of trace elements, 20 parts of modified starch, and 0.3 parts of microbial bacterial liquid.

[0116] The preparation method of the mineral source humic acid slurry is as follows:

[0117] (1) Preparation of mineral source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, stir for 1 h under the condition of constant temperature at 90 - 100 °C to obtain solution A; Add formaldehyde accounting for 4% of the mass of humic acid and sodium sulfite accounting for 8% of the mass of humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 h, and after sterilization, the mineral source humic acid slurry is obtained.

[0118] In this comparative example, except for only using the mineral source humic acid slurry, the other raw materials and preparation methods are the same as those in Example 1.

[0119] The product performance is detected according to the corresponding method in the national quality standard NY525 - 2021 for organic fertilizers. The organic matter of the product is ≥ 40%, the moisture is ≤ 10%, the pH is 6 - 9, and the effective viable count of bacteria is ≥ 100 million / g.

[0120] Comparative Example 2

[0121] A spraying mineral source bio - organic fertilizer comprises the following raw materials in parts by weight: 70 parts of bio - humic acid slurry, 0.4 part of trace elements, 20 parts of modified starch, and 0.3 part of microbial bacteria liquid.

[0122] The preparation method of the bio - humic acid slurry is as follows:

[0123] (1) Preparation of bio - humic acid slurry: First, use a ball mill to crush and screen lignite to obtain a sample with a particle size of 0.125 - 0.250 mm, then soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; Take 90 g of the pretreated coal sample, add 10 mL of composite fermentation bacteria liquid and 30 g of nutrient material, mix evenly, adjust the moisture content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation is completed, the composite humic acid slurry is obtained.

[0124] In this comparative example, except for only using the bio - humic acid slurry, the other raw materials and preparation methods are the same as those in Example 1.

[0125] The product performance is detected according to the corresponding method in the national quality standard NY525 - 2021 for organic fertilizers. The organic matter of the product is ≥ 50%, the moisture is ≤ 10%, the pH is 6 - 9, and the effective viable count of bacteria is ≥ 100 million / g.

[0126] Comparative Example 3

[0127] A spraying mineral source bio - organic fertilizer comprises the following raw materials in parts by weight: 70 parts of composite humic acid slurry, 0.4 part of trace elements, 20 parts of modified starch, and 0.3 part of microbial bacteria liquid.

[0128] The composite humic acid slurry contains a mineral-source humic acid slurry and a biological humic acid slurry, and its preparation method is as follows:

[0129] (1) Preparation of the mineral-source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, stir for 1 h under the condition of constant temperature at 90 - 100 °C to obtain solution A; add formaldehyde accounting for 4% of the mass of humic acid and sodium sulfite accounting for 8% of the mass of humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 h, and after sterilization, the mineral-source humic acid slurry is obtained;

[0130] (2) Preparation of the biological humic acid slurry: First, crush lignite with a ball mill and perform screening to obtain a sample with a particle size of 0.125 - 0.250 mm, then soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; take 80 g of the pretreated coal sample, 10 g of the mineral-source humic acid slurry, add 10 mL of fermentation bacterial liquid and 30 g of nutrient material, mix evenly, adjust the water content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation is completed, the composite humic acid slurry is obtained.

[0131] The fermentation bacterial liquid in step (2) is Bacillus subtilis, and its specific preparation method is as follows: Inoculate the cryopreserved Bacillus subtilis onto an LB solid medium by the streak plate method, culture it at a suitable temperature of 25 - 30 °C for 48 h, then pick a loop of colonies and inoculate them into an LB liquid medium, and in a constant temperature shaker, under the conditions of 25 - 30 °C and 120 r / min, shake and culture for 48 h to obtain a bacterial liquid; then inoculate the mixed bacterial liquid into 1 L of LB liquid medium at an inoculation amount of 5%, and expand the culture in a constant temperature shaker at 25 - 30 °C for 96 h to obtain the fermentation bacterial liquid.

[0132] In this comparative example, except that only Bacillus subtilis is used in the preparation of the biological humic acid slurry, the other raw materials and preparation methods are the same as those in Example 1.

[0133] Detect the product performance according to the corresponding method in the national quality standard NY525 - 2021 for organic fertilizers. The organic matter of the product is ≥50%, the moisture is ≤10%, the pH is 6 - 9, and the effective viable bacteria count is ≥100 million / g.

[0134] Comparative Example 4

[0135] A sprayed mineral-source biological organic fertilizer comprises the following raw materials in parts by weight: 70 parts of composite humic acid slurry, 0.4 part of trace elements, 20 parts of modified starch, and 0.3 part of microbial bacterial liquid.

[0136] The composite humic acid slurry contains a mineral-source humic acid slurry and a biological humic acid slurry, and its preparation method is as follows:

[0137] (1) Preparation of the mineral-source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, and stir for 1 h under the condition of constant temperature at 90 - 100 °C to obtain solution A; Add formaldehyde accounting for 4% of the mass of humic acid and sodium sulfite accounting for 8% of the mass of humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 h, and after sterilization, the mineral-source humic acid slurry is obtained.

[0138] (2) Preparation of the biological humic acid slurry: First, crush lignite using a ball mill and perform screening to obtain a sample with a particle size of 0.125 - 0.250 mm. Then, soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; Take 80 g of the pretreated coal sample, 10 g of the mineral-source humic acid slurry, add 10 mL of fermentation bacterial liquid and 30 g of nutrient material, mix evenly, adjust the moisture content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation ends, the composite humic acid slurry is obtained.

[0139] The fermentation bacterial liquid in step (2) is Pseudomonas yamanorum, and the specific preparation method is as follows: Inoculate the cryopreserved Pseudomonas yamanorum onto an LB solid medium by the streak plate method, culture it at a suitable temperature of 25 - 30 °C for 48 h, then pick a loop of colonies and inoculate them into an LB liquid medium, and shake culture at 25 - 30 °C and 120 r / min in a constant-temperature shaker for 48 h to obtain a bacterial liquid; Then inoculate the mixed bacterial liquid into 1 L of LB liquid medium at an inoculation amount of 5% and perform enlarged culture in a constant-temperature shaker at 25 - 30 °C for 96 h to obtain the fermentation bacterial liquid.

[0140] In this comparative example, except that only Pseudomonas yamanorum is used in the preparation of the biological humic acid slurry, the other raw materials and preparation methods are the same as those in Example 1.

[0141] Detect the product performance according to the corresponding method in the national quality standard NY525 - 2021 for organic fertilizers. The organic matter of the product is ≥ 50%, the moisture is ≤ 10%, the pH is 6 - 9, and the effective viable bacteria count is ≥ 100 million / g.

[0142] Comparative Example 5

[0143] A spraying mineral-source biological organic fertilizer comprises the following raw materials in parts by weight: 70 parts of composite humic acid slurry, 0.4 part of trace elements, 20 parts of modified starch, and 0.3 part of microbial bacterial liquid.

[0144] The composite humic acid slurry contains mineral-source humic acid slurry and biological humic acid slurry, and its preparation method is as follows:

[0145] (1) Preparation of mineral-source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, stir for 1 h under the condition of constant temperature at 90 - 100 °C to obtain solution A; Add formaldehyde accounting for 4% of the mass of humic acid and sodium sulfite accounting for 8% of the mass of humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 h, and after sterilization, the mineral-source humic acid slurry is obtained;

[0146] (2) Preparation of biological humic acid slurry: First, crush lignite with a ball mill and screen it to obtain a sample with a particle size of 0.125 - 0.250 mm, then soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; Take 80 g of the pretreated coal sample, 10 g of the mineral-source humic acid slurry, add 10 mL of fermentation bacterial liquid and 30 g of nutrient material, mix evenly, adjust the water content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation is completed, the composite humic acid slurry is obtained.

[0147] The fermentation bacterial liquid in step (2) is Bacillus thuringiensis, and the specific preparation method is as follows: Inoculate the cryopreserved Bacillus thuringiensis onto an LB solid medium by the method of streaking on a plate, culture it at a suitable temperature of 25 - 30 °C for 48 h, then pick a loop of colonies and inoculate them into an LB liquid medium, and in a constant-temperature shaker, under the conditions of 25 - 30 °C and 120 r / min, shake and culture for 48 h to obtain a bacterial liquid; Then inoculate the mixed bacterial liquid into 1 L of LB liquid medium at an inoculation amount of 5%, and expand the culture in a constant-temperature shaker at 25 - 30 °C for 96 h to obtain the fermentation bacterial liquid.

[0148] In this comparative example, except that only Bacillus thuringiensis is used in the preparation of the biological humic acid slurry, the other raw materials and preparation methods are the same as those in Example 1.

[0149] Detect the product performance according to the corresponding method in the national quality standard NY525 - 2021 for organic fertilizers. The organic matter content of the product is ≥50%, the moisture content is ≤10%, the pH is 6 - 9, and the effective viable bacteria count is ≥100 million / g.

[0150] Comparative Example 6

[0151] A spraying mineral-source biological organic fertilizer comprises the following raw materials in parts by weight: 70 parts of composite humic acid slurry, 0.4 part of trace elements, 20 parts of modified starch, and 0.3 part of microbial bacterial liquid.

[0152] The composite humic acid slurry contains a mineral-source humic acid slurry and a biological humic acid slurry, and its preparation method is as follows:

[0153] (1) Preparation of the mineral-source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, and stir for 1 h under the condition of constant temperature at 90 - 100 °C to obtain solution A; Add formaldehyde accounting for 4% of the mass of humic acid and sodium sulfite accounting for 8% of the mass of humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 h, and after sterilization, the mineral-source humic acid slurry is obtained;

[0154] (2) Preparation of the biological humic acid slurry: First, use a ball mill to crush and screen lignite to obtain a sample with a particle size of 0.125 - 0.250 mm, then soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; Take 80 g of the pretreated coal sample, 10 g of the mineral-source humic acid slurry, add 10 mL of a composite fermentation bacterial liquid and 30 g of a nutrient material, mix evenly, adjust the water content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation is completed, the composite humic acid slurry is obtained.

[0155] The composite fermentation bacterial liquid in step (2) is composed of Pseudomonas yamanorum and Bacillus subtilis, and the volume ratio of the three is 1:1. The specific preparation method is as follows: Inoculate the cryopreserved Pseudomonas yamanorum and Bacillus subtilis onto an LB solid medium by the method of streak plating respectively, culture at a suitable temperature of 25 - 30 °C for 48 h, then take a loop of colonies and inoculate them into an LB liquid medium respectively, and carry out shaking culture in a constant-temperature shaker at 25 - 30 °C and 120 r / min for 48 h to obtain three bacterial liquids; Mix the two bacterial liquids evenly according to a volume ratio of 1:1, then inoculate the mixed bacterial liquid into 1 L of LB liquid medium at an inoculation amount of 5%, and carry out enlarged culture in a constant-temperature shaker at 25 - 30 °C for 96 h to obtain the composite fermentation bacterial liquid.

[0156] In this comparative example, except that in the preparation of the biological humic acid slurry, only Pseudomonas yamanorum and Bacillus subtilis are used, the other raw materials and preparation methods are the same as those in Example 1.

[0157] Detect the product performance according to the corresponding method in the national quality standard NY525 - 2021 for organic fertilizers. The organic matter of the product is ≥60%, the moisture is ≤10%, the pH is 6 - 9, and the effective viable bacteria count is ≥100 million / g.

[0158] Comparative Example 7

[0159] A spraying slurry bio-organic fertilizer, comprising the following raw materials in parts by weight: 70 parts of compound humic acid slurry, 0.4 part of trace elements, 20 parts of modified starch, and 0.3 part of microbial bacteria liquid.

[0160] The compound humic acid slurry comprises a mineral source humic acid slurry and a biological humic acid slurry, and its preparation method is as follows:

[0161] (1) Preparation of mineral source humic acid slurry: Dissolve 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, stir at a constant temperature of 90 - 100 °C for 1 h to obtain solution A; add 4% of formaldehyde by mass of humic acid and 8% of sodium sulfite by mass of humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 h, and after sterilization, the mineral source humic acid slurry is obtained;

[0162] (2) Preparation of biological humic acid slurry: First, use a ball mill to crush and screen lignite to obtain a sample with a particle size of 0.125 - 0.250 mm, then soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize to obtain a pretreated coal sample; take 80 g of the pretreated coal sample, 10 g of the mineral source humic acid slurry, add 10 mL of compound fermentation bacteria liquid and 30 g of nutrient material, mix evenly, adjust the moisture content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation is completed, the compound humic acid slurry is obtained.

[0163] The compound fermentation bacteria liquid in step (2) is composed of Bacillus thuringiensis and Bacillus subtilis, and the volume ratio of the three is 1:1. The specific preparation method is as follows: Inoculate the cryopreserved Pseudomonas yamanensis and Bacillus subtilis onto an LB solid medium by the method of streak plating respectively, culture at a suitable temperature of 25 - 30 °C for 48 h, then take one loop of colonies and inoculate them into an LB liquid medium respectively, and in a constant temperature shaker, under the conditions of 25 - 30 °C and 120 r / min, shake and culture for 48 h to obtain three kinds of bacteria liquids; mix the two kinds of bacteria liquids evenly according to a volume ratio of 1:1, then inoculate the mixed bacteria liquid into 1 L of LB liquid medium at an inoculation amount of 5%, and expand the culture in a constant temperature shaker at 25 - 30 °C for 96 h to obtain the compound fermentation bacteria liquid.

[0164] In this comparative example, except that in the preparation of the biological humic acid slurry, only Bacillus thuringiensis and Bacillus subtilis are used, the other raw materials and preparation methods are the same as those in Example 1.

[0165] The product performance was detected according to the corresponding method in the national quality standard of organic fertilizer NY525-2021. The organic matter of the product is ≥60%, the moisture is ≤10%, the pH is 6-9, and the effective viable count is ≥100 million / g.

[0166] Comparative Example 8

[0167] A sprayed bio-organic fertilizer from mineral sources, comprising the following raw materials in parts by weight: 70 parts of compound humic acid slurry, 0.4 part of trace elements, 20 parts of modified starch, and 0.3 part of microbial bacterial liquid.

[0168] The compound humic acid slurry comprises a mineral source humic acid slurry and a biological humic acid slurry, and its preparation method is as follows:

[0169] (1) Preparation of mineral source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9-10, stir at a constant temperature of 90-100 °C for 1 h to obtain solution A; add 4% of the mass of formaldehyde and 8% of the mass of sodium sulfite of humic acid to solution A, stir at 90-100 °C and 0.1 MPa for 1 h, and after sterilization, the mineral source humic acid slurry is obtained;

[0170] (2) Preparation of biological humic acid slurry: First, crush lignite with a ball mill and screen it to obtain a sample with a particle size of 0.125-0.250 mm. Then, soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; take 80 g of the pretreated coal sample, 10 g of the mineral source humic acid slurry, add 10 mL of compound fermentation bacterial liquid and 30 g of nutrient material, mix evenly, adjust the moisture content to 60-70%, and carry out mixed fermentation at 26-30 °C for 10 d. After the fermentation is completed, the compound humic acid slurry is obtained.

[0171] The compound fermentation bacterial liquid in step (2) is composed of Bacillus thuringiensis and Pseudomonas yamanorum, and the volume ratio of the three is 1:1. The specific preparation method is as follows: Inoculate the cryopreserved Pseudomonas yamanorum and Bacillus subtilis onto an LB solid medium by the method of streaking for isolation respectively, culture at a suitable temperature of 25-30 °C for 48 h, then take one loop of colonies respectively and inoculate them into an LB liquid medium, and in a constant temperature shaker, under the conditions of 25-30 °C and 120 r / min, oscillate and culture for 48 h to obtain three kinds of bacterial liquids; mix the two kinds of bacterial liquids evenly according to a volume ratio of 1:1, and then inoculate the mixed bacterial liquid into 1 L of LB liquid medium at an inoculation amount of 5%, and expand the culture in a constant temperature shaker at 25-30 °C for 96 h to obtain the compound fermentation bacterial liquid.

[0172] In this comparative example, except that only Bacillus thuringiensis and Pseudomonas yamana were used in the preparation of the biological humic acid slurry, the remaining raw materials and preparation methods were the same as those in Example 1.

[0173] The product performance was detected according to the corresponding method in the national quality standard NY525-2021 for organic fertilizers. The product had an organic matter content of ≥60%, a moisture content of ≤10%, a pH of 6-9, and an effective viable count of ≥100 million / g.

[0174] Performance test

[0175] Test on the lignite degradation ability of strains

[0176] Pseudomonas yamana, Bacillus subtilis, and Bacillus thuringiensis stored frozen were respectively inoculated onto LB solid medium by the streak plate method and cultured in a constant temperature incubator at 25-30 °C. After the cultured microorganisms covered the plate, 0.8 g of lignite samples were evenly sprinkled on the surface of the bacteria in each plate. After the coal particles were evenly attached to the bacteria, they were placed in an inverted position in a constant temperature incubator at 28-30 °C for culturing. After 72 h, the effect of the microorganisms in the solid medium liquefying the lignite was observed, and the degradation effect was judged based on whether black droplets appeared on the surface of the coal sample. From Figure 2 it can be seen that obvious droplets appeared on the strain plates, indicating that the three strains all had a degradation effect on Zhaotong lignite.

[0177] To further verify the degradation ability of different strains, the experimental groups were further set up for quantitative testing according to the above method:

[0178] s1: Pseudomonas yamana;

[0179] s2: Bacillus subtilis;

[0180] s3: Bacillus thuringiensis;

[0181] s4: Pseudomonas yamana + Bacillus subtilis;

[0182] s5: Bacillus subtilis + Bacillus thuringiensis;

[0183] s6: Pseudomonas yamana + Bacillus thuringiensis;

[0184] s7: Bacillus subtilis + Pseudomonas yamana + Bacillus thuringiensis

[0185] After the reaction ended, the residual coal samples on the plate were rinsed off with deionized water. The rinsing solution was centrifuged at 10000 r / min for 10 min, and then the precipitate was collected and dried in a constant temperature oven at 70 °C to a constant mass, and the degradation rate and free humic acid content of the lignite were analyzed.

[0186] Measure the degradation rate and the content of free humic acid in lignite before and after the reaction. Determine the content of free humic acid in lignite according to GB / T 35107—2017 Determination of Soluble Humic Acid Content in Mineral Humic Acid Fertilizers. The calculation method of the degradation rate (η) is as follows:

[0187] η = 00%;

[0188] where m0 is the mass of lignite before the reaction, g; m1 is the mass of lignite after the reaction, g.

[0189] The test results are shown in Table 1:

[0190] Table 1 Test Results

[0191]

[0192] From the data in the table, we can see that all three strains of the present invention can increase the content of free humic acid. When the three strains are used together, the content of free humic acid can be effectively increased. The increase in the content of free humic acid can, on the one hand, improve the physical, chemical and biological properties of the soil, and on the other hand, promote the growth and development of crops and improve the yield and quality, realizing the improvement of the overall fertilizer efficiency.

[0193] Pot experiment:

[0194] Explore the effect of fertilizers on the growth of crops through pot experiments. Select the Chinese cabbage variety "Aijiangjun" for pot experiment planting. A total of 14 treatment groups are set in the experiment:

[0195] The first group is the blank control group without adding fertilizers; the second group is the inorganic fertilizer treatment group. According to the optimal fertilization ratio of Chinese cabbage N:P2O5:K2O = 1:0.35:0.6, set the chemical fertilizer application amounts as N 150 mg / kg, P2O5 52.5 mg / kg, and K2O 90 mg / kg, and convert them into the corresponding amounts of urea, superphosphate and potassium sulfate respectively.

[0196] Groups 3-6 are the example fertilizer groups, with a usage amount of 200 mg / kg soil.

[0197] Groups 7-14 are the comparative example fertilizer groups, with a usage amount of 200 mg / kg soil.

[0198] The plastic pots selected for the experiment had a specification of 160 mm × 115 mm (upper diameter × height). Weigh 1.5 g of air-dried soil, allocate the nitrogen, phosphorus, potassium fertilizers and the mineral source humic acid bacterial agent for each pot according to the dosage set in the experiment. After stirring and mixing evenly, put them into plastic flower pots and water them according to 70% of the maximum water holding capacity in the field. After numbering the potted plants, arrange them randomly. Start sowing after the soil has been placed for 24 hours. Evenly sprinkle 20 small Chinese cabbage seeds into each pot. Thin out the seedlings 10 days after the small Chinese cabbage has emerged, and keep 4 well-growing seedlings in each pot for subsequent monitoring. During the growth period of the small Chinese cabbage, change the position of the potted plants every day to ensure that the small Chinese cabbage receives uniform light. Each treatment was set with 10 replicates.

[0199] By measuring the biological traits of the small Chinese cabbage samples, evaluate the effects of different fertilizers on its growth, yield and quality. Collect representative small Chinese cabbage plants at the mature stage for measurement and recording of biological traits, including plant height, spread, maximum leaf length, maximum leaf width and single plant weight. The soluble protein content of small Chinese cabbage was determined by the Coomassie brilliant blue G-250 colorimetric method, and the soluble sugar content was determined by the anthrone colorimetric method. The average value was taken for all test results.

[0200] Table 1 Small Chinese cabbage indicators

[0201]

[0202] From the data in Table 1, we can see that all the indicators of the small Chinese cabbage obtained in the example group of the present invention are better than those of the blank control group, the inorganic fertilizer group and the comparative example group. This shows that the organic biological fertilizer of the present invention can completely replace the use of chemical fertilizers, which also shows that Pseudomonas yamanensis, Bacillus subtilis and Bacillus thuringiensis ferment lignite sufficiently, increasing the content of free humic acid and having a more significant promoting effect on crops. For the comparative examples 3-8 with the changed microbial composition and the comparative examples 1-2 with the changed composite humic acid components, their various performances have decreased to varying degrees. Each raw material and composition of the present invention is an organic whole, which is the key to achieving the technical effect, and the effect will be weakened if any one is missing.

[0203] Table 3 Small Chinese cabbage quality indicators

[0204]

[0205] Vitamin C, soluble protein and titratable acid in vegetables are often used as indicators to evaluate the quality of vegetables. The titratable acid content is not affected much by each experimental group, and the vitamin C and soluble protein indicators of the examples are better than those of the blank control group, the inorganic fertilizer group and the comparative example group.

[0206] It should be noted that the above embodiments are only some of the embodiments of the preferred ways to implement the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of the present invention.

Claims

1. A sprayed slurry bio-organic fertilizer from mineral resources, characterized in that, It comprises the following raw materials in parts by weight: 70 - 80 parts of compound humic acid slurry, 0.1 - 0.5 parts of trace elements, 10 - 20 parts of modified starch, and 0.1 - 0.5 parts of microbial bacterial liquid; The compound humic acid slurry includes mineral - source humic acid slurry and biological humic acid slurry, and its preparation method is as follows: (1) Preparation of mineral - source humic acid slurry: Disperse 10 g of humic acid in 50 ml of deionized water, add potassium hydroxide to adjust the pH to 9 - 10, stir at a constant temperature of 90 - 100 °C for 1 - 3 h to obtain solution A; Add 4% of formaldehyde by mass of humic acid and 8% of sodium sulfite by mass of humic acid to solution A, stir at 90 - 100 °C and 0.1 MPa for 1 - 3 h, and after sterilization, the mineral - source humic acid slurry is obtained; (2) Preparation of biological humic acid slurry: First, crush lignite with a ball mill and screen it to obtain a sample with a particle size of 0.125 - 0.250 mm. Then, soak and oxidize the sample with 10 mol / L nitric acid for 48 h, wash it with deionized water until neutral, dry and sterilize it to obtain a pretreated coal sample; Take 80 g of the pretreated coal sample, 10 g of mineral - source humic acid slurry, add 10 mL of compound fermentation bacterial liquid and 30 g of nutrient material, mix evenly, adjust the moisture content to 60 - 70%, and carry out mixed fermentation at 26 - 30 °C for 10 d. After the fermentation ends, the compound humic acid slurry is obtained; Step (2) The compound fermentation bacterial liquid is composed of Pseudomonas yamana ( Pseudomonas yamanorum ), Bacillus subtilis ( Bacillus subtilis ), and Bacillus thuringiensis ( Bacillus thuringiensis ), and the volume ratio of the three is 1:1:

1. The specific preparation method is as follows: The cryopreserved Pseudomonas yamana, Bacillus subtilis, and Bacillus thuringiensis are respectively inoculated onto LB solid medium by the streak plate method and cultured at 25-30 °C for 48 h. Then, one loop of colonies is respectively inoculated into LB liquid medium and cultured in a constant temperature shaker at 25-30 °C and 120 r / min for 48 h to obtain three kinds of bacterial liquids; the three kinds of bacterial liquids are mixed evenly according to the volume ratio of 1:1:1, and then the mixed bacterial liquid is inoculated into 1 L of LB liquid medium at an inoculation amount of 5% and expanded and cultured in a constant temperature shaker at 25-30 °C for 96 h to obtain the compound fermentation bacterial liquid.

2. The sprayed slurry bio-organic fertilizer according to claim 1, characterized in that, The trace elements are one or more of boric acid, zinc sulfate, ferrous sulfate, and manganese sulfate.

3. The sprayed slurry bio-organic fertilizer according to claim 1, characterized in that, The microbial bacterial liquid is one or more of Bacillus licheniformis, Trichoderma harzianum, and Paenibacillus megaterium.

4. The sprayed slurry bio-organic fertilizer according to claim 1, wherein The strain number of the Pseudomonas yamanorum is CGMCC No.1.16088, and the strain number of the Bacillus thuringiensis is CGMCC No.1.8790, both of which are purchased from the China General Microbiological Culture Collection Center; The preservation number of the Bacillus subtilis is CGMCC No.44482, which is preserved in the China General Microbiological Culture Collection Center. The preservation date is September 12, 2023, and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

5. The preparation method of the sprayed bio-organic fertilizer from ore source according to any one of claims 1-4, characterized in that, It includes the following steps: (1) Mix and stir the compound humic acid slurry, trace elements, and modified starch in parts by weight evenly to obtain a spraying slurry, and convey the slurry to a granulator for spray granulation; (2) Screening: The particles are screened through a rotary screen, and the particles with a particle size of 2 mm - 4 mm are selected as finished - product particles, and those less than 2 mm or greater than 4 mm are re - worked; (3) Cooling: Roll the dried particles in a cooling drum for cooling treatment, and the cooling time is 10 min - 30 min; (4) Adding bacteria: After cooling, the particles enter the drum, and the microbial bacterial liquid is atomized and evenly coated on the surface of the particles; (5) Packaging and warehousing: The obtained particles are inspected, packaged, and warehoused to obtain the finished - product biological organic fertilizer.

6. The preparation method of the sprayed bio-organic fertilizer from mineral resources according to claim 5, characterized in that, In step (1) of spraying granulation, the head temperature is 400 - 450 °C, the tail temperature is 60 - 65 °C, and the spraying volume is 10 - 15 m 3 / h.

Images