Impregnation liquid and preparation method thereof, antibacterial fiber, antibacterial fabric and application

Abstract

The invention discloses a surface glycosylation modified impregnation liquid and a preparation method thereof, an antibacterial fiber, an antibacterial fabric and application, and belongs to the technical field of antibiosis. According to the preparation method, a reactive main chain polymer and amino sugar are adopted, steeping liquor is prepared through step-by-step reaction or one-pot reaction, and then glycosylation modification is conducted on the surface of the nano-antibacterial fiber / fabric. On the basis of the nano antibacterial fiber, the fiber surface is subjected to glycosylation modification, the balance between the biological safety and the antibacterial activity of the nano antibacterial fiber material is realized, and the graphene fiber capable of selectively capturing and enriching pathogenic microorganisms such as bacteria and the like is obtained by utilizing the specific binding of a characteristic sugar chain to the bacteria; furthermore, the killing efficiency of the nano-antibacterial fiber on dispersed pathogenic microorganisms in the environment is greatly improved, the problem of space limitation of a nano-antibacterial material in contact antibiosis is effectively solved, and the material is suitable for preparing active bacterium targeting type drug-resistant bacterium infection wound dressings.

Description

Technical Field

[0001] The present application belongs to the field of antibacterial technology, and specifically relates to an impregnation liquid and a preparation method thereof, antibacterial fibers, antibacterial fabrics and applications. Background Art

[0002] Chronic wounds caused by drug-resistant bacteria have always been a problem that needs to be solved in the clinic. Using the nanoknife effect, photothermal effect, and photofree effect of materials to physically kill bacteria on the wound surface is one of the main methods to solve wound infection caused by drug-resistant bacteria. At present, wound dressings containing nanosilver, nanogold, graphene and other ingredients have been widely used in clinical treatment.

[0003] Considering the toxicity of nanomaterials themselves, the current safer strategy is to fix nanomaterials on the surface of dressings, so as to kill bacteria locally while avoiding the potential safety risks caused by the diffusion of nanomaterials into the blood circulation. However, this fixation modification strategy also brings about the spatial limitation of the bacterial contact killing effect: due to the wet environment characteristics of cell tissues, both the photothermal effect and the free radicals generated by light excitation can only effectively kill bacteria within a very small range, because heat energy and free radicals will be rapidly dissipated and quenched in the water environment, resulting in unsatisfactory treatment effects for bacterially infected wounds. Summary of the invention

[0004] In response to the spatial limitation problem caused by the passive antibacterial effect of wound dressings in the prior art, the present application provides an impregnation liquid and a preparation method thereof, antibacterial fibers, antibacterial fabrics and applications, which utilize the specific recognition of characteristic sugar chains and bacterial surface lectins to achieve active adhesion and enrichment of bacteria by fiber materials, thereby overcoming the spatial limitation problem existing in the physical antibacterial process.

[0005] In order to solve the above problems, the technical solutions adopted in this application are as follows: In a first aspect, the present application provides an impregnation liquid, wherein the impregnation liquid comprises a component having a structural formula as shown in formula (1): Formula (1) In formula (1), x and y are independently selected from integers between 1 and 100, and n1 is selected from integers between 1 and 20; preferably, x is 50 to 80, and y is 20 to 50; R1 is a hydrogen atom or a hydrocarbon group, and R2 is a hydrogen atom, a hydrocarbon group, an amino group, a hydroxyl group, or an atom or an atomic group that can replace the above groups.

[0006] In a second aspect, the present application provides a method for preparing the above-mentioned impregnation solution, comprising mixing compound I, compound II, and compound III to obtain an impregnation solution; The structural formula of the compound I is shown in formula (2): Formula (2) In formula (2), m is an integer between 50 and 150; The structural formula of the compound II is shown in formula (3): Formula (3); In formula (3), R3 is a hydrogen atom or a hydrocarbon group, and R4 is a hydrogen atom, a hydrocarbon group, an amino group, a hydroxyl group, or an atom or an atomic group that can replace the above groups; The structural formula of the compound III is shown in formula (4) or formula (5): Formula (4), Formula (5) In formula (4), n2 is an integer between 1 and 20; In formula (5), n2 and n3 are independently selected from integers between 1 and 20, and P is an oxygen atom, a carbon atom, a nitrogen atom, or a sulfur atom.

[0007] In the second aspect of the present application, the compound III is amino-modified glucose, mannose, maltose, galactose or lactose.

[0008] In the second aspect of the present application, compound I and compound III are mixed, a solvent is added to dissolve the reactants, and the mixture is stirred for a first reaction, and then compound II is added and stirred for a second reaction to obtain the impregnation solution.

[0009] In the second aspect of the present application, the conditions of the first reaction are reaction temperature of 10-50° C., reaction time of 0.5-5 hours, and reaction pH of 8-10.

[0010] In the second aspect of the present application, the conditions of the second reaction are reaction temperature of 10-50° C., reaction time of 0.5-5 hours, and reaction pH of 8-10.

[0011] In a third aspect, the present application provides an antibacterial fiber, which is formed by impregnating nano antibacterial fibers with the impregnation liquid described in the first aspect, wherein the nano antibacterial fibers are nano silver antibacterial fibers, nano gold antibacterial fibers or graphene antibacterial fibers.

[0012] In a fourth aspect, the present application provides an antibacterial fabric, which is woven from antibacterial fibers obtained by impregnating nano-antibacterial fibers with the impregnation solution described in the first aspect above, or is woven from nano-antibacterial fabric impregnated with the impregnation solution described in the first aspect above; the nano-antibacterial fibers are nano-silver antibacterial fibers, nano-gold antibacterial fibers or graphene antibacterial fibers, and the nano-antibacterial fabric is nano-silver antibacterial fabric, nano-gold antibacterial fabric or graphene antibacterial fabric.

[0013] In a fifth aspect, the present application provides use of the antibacterial fabric described in the fourth aspect in the preparation of an antibacterial dressing.

[0014] Compared with the prior art, the beneficial effects of the present application are at least: Based on nano-antibacterial fibers, the present application achieves a balance between the biosafety and antibacterial activity of nano-antibacterial fiber materials by glycosylation modification of the fiber surface, and utilizes the specific binding of characteristic sugar chains to bacteria to obtain graphene fibers that can selectively capture and enrich bacteria and other pathogenic microorganisms, thereby greatly improving the killing efficiency of nano-antibacterial fibers on pathogenic microorganisms dispersed in the environment, effectively overcoming the spatial limitation problem of nano-antibacterial materials in contact antibacterial treatment, and the material is suitable for the preparation of bacteria-active targeted wound dressings for drug-resistant bacteria infections.

[0015] The remaining advantages of the present application will be partially explained in the following description, and some will be obvious from the description, or can be learned through the implementation of the embodiments of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the H-NMR spectrum (DMSO-d6) of the reactive main-chain polymer; Figure 2 This is the H-NMR spectrum (D2O) of the sugar chain polymer containing lactose structure in the side chain; Figure 3 This is a laser confocal microscope photo; Figure 4 The results of in vitro antibacterial experiments; Figure 5 The experimental results of wound treatment model in mice infected with drug-resistant bacteria; Figure 6 The results of the cytotoxicity and bacterial enrichment experiments of graphene fiber materials; Figure 7 These are the XPS test results of the fiber surface glycosylation modification bonding strength experiment. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme of the embodiments of the present application will be clearly and completely described below in conjunction with the drawings of the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. Based on the described embodiments of the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0018] For the purpose of the present application, it should be understood that, unless explicitly indicated otherwise, the present application may adopt various alternative changes and step sequences. In addition, except in any operating examples or otherwise specified, all numerical values ​​representing the amount of the composition used in the specification and claims should be understood to be modified by the term "about" in all cases. Therefore, unless otherwise specified, the numerical parameters listed in the following specification and the appended claims are approximate values, which can vary according to the desired properties to be obtained by the present application. At least, it is not intended to limit the scope of the application of the doctrine of equivalents to the claims, and each numerical parameter should at least be interpreted according to the numerical value of the significant figures reported and by applying common rounding techniques.

[0019] Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the disclosure are approximations, the numerical values ​​set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contains certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

[0020] Graphene is a two-dimensional (2D) material composed of single-atom-thick carbon atoms tightly stacked into a honeycomb shape. It has good mechanical, thermal and electrical properties, which makes it widely used. As a derivative of graphene, graphene also has a large number of hydrophilic groups such as carboxyl and hydroxyl groups on its surface, which allows it to be stably dispersed in water and form a stable and uniform solution. The researchers studied the biocompatibility, cytotoxicity and antibacterial properties of graphene. The results showed that graphene has excellent biocompatibility, and its unique nanostructure makes it have good antibacterial properties and will not produce drug resistance to bacteria. At the same time, the results show that graphene can effectively promote cell proliferation.

[0021] Fixing the graphene-containing material on the surface of the dressing can prevent the nanomaterial from diffusing into the blood circulation while killing bacteria locally. However, due to spatial limitations, its killing effect on bacteria is limited. Other nano antibacterial materials such as nanosilver and nanogold also have the same problem. In order to solve the above problems, the present application provides an impregnation liquid, and uses the impregnation liquid to make antibacterial fibers / fabrics, so as to achieve active adhesion and enrichment of bacteria by fiber materials, thereby overcoming the spatial limitation problem in the physical antibacterial process.

[0022] The following is a detailed introduction to this plan: The present application example provides a surface glycosylation-modified impregnation liquid, which introduces a sugar chain structure into the side chain of a reactive main chain polymer to obtain a side chain sugar-containing polymer, which is the effective component of the impregnation liquid. The present application example also provides an antibacterial fiber / fabric, which uses the above impregnation liquid or its effective component to modify the surface of a nano antibacterial fiber to obtain a target antibacterial fiber / fabric.

[0023] The impregnation solution includes an effective component having a structural formula as shown in formula (1): Formula (1) In formula (1), x and y are independently selected from integers between 1 and 100, and n1 is selected from integers between 1 and 20; preferably, x is 50 to 80, and y is 20 to 50; R1 is a hydrogen atom or a hydrocarbon group, and R2 is a hydrogen atom, a hydrocarbon group, an amino group, a hydroxyl group, or an atom or an atomic group that can replace the above groups.

[0024] In the fiber / fabric impregnation solution provided in the embodiment of the present application, the effective component represented by formula (1) is a copolymer coupled with an NHS active ester, one monomer of which is an amino sugar and the other monomer is dopamine or a dopamine derivative.

[0025] The embodiment of the present application provides a method for preparing an impregnation liquid, which comprises mixing compound I, compound II, and compound III to obtain an impregnation liquid; The structural formula of the compound I is shown in formula (2): Formula (2) In formula (2), m is an integer between 50 and 150; The structural formula of the compound II is shown in formula (3): Formula (3); In formula (3), R3 is a hydrogen atom or a hydrocarbon group, and R4 is a hydrogen atom, a hydrocarbon group, an amino group, a hydroxyl group, or an atom or an atomic group that can replace the above groups; The structural formula of the compound III is shown in formula (4) or formula (5): Formula (4), Formula (5) In formula (4), n2 is an integer between 1 and 20; In formula (5), n2 and n3 are independently selected from integers between 1 and 20, and P is an oxygen atom, a carbon atom, a nitrogen atom, or a sulfur atom.

[0026] Compound I provided in the examples of the present application is a reactive main-chain polymer, and its side chain contains an NHS active ester. The following scheme can be used for synthesis: a monomer (CAS: 38862-24-7) and a RAFT agent (CAS: 461642-78-4 or 26502-29-0) are subjected to RAFT polymerization in the presence of an initiator (CAS: 78-67-1) and a solvent, and the molar ratio of the polymerization (initiator: RAFT agent: monomer) can be 0.2 / 1 / 25, 0.2 / 1 / 50, 0.2 The reaction temperature can be 60°C, 65°C, 70°C, 75°C, 80°C, etc., which are not limited here.

[0027] The compound II provided in the embodiment of the present application is dopamine or a dopamine derivative. If it is a derivative, the original amino and hydroxyl groups of dopamine should be retained in its structure. The purpose of retaining the amino group is not to affect the synthesis of the component shown in formula (1), and the purpose of retaining the hydroxyl group is not to affect the interfacial adhesion between the hydroxyl group and the nano-antibacterial fiber. The atom or atomic group replacing the hydrogen atom should not affect the synthesis of the component shown in formula (1), and should not affect the interfacial adhesion between the component shown in formula (1) and the nano-antibacterial fiber.

[0028] Compound III provided in the examples of the present application is an amino sugar, i.e., a compound in which several hydroxyl groups of the sugar are replaced by amino groups, and the substituted sugar type is at least one of a monosaccharide, a disaccharide, or a polysaccharide, such as glucose, mannose, maltose, galactose, or lactose, etc. The preparation of amino sugars is a prior art, and can be carried out with reference to the methods reported in the prior literature, for example, adding sugar to an aqueous solution of ammonium carbamate, the concentration of ammonium carbamate in the reaction solution is 16 M, the concentration of sugar is 0.2 M, stirring at room temperature for 24 hours, and after the reaction is completed, the reaction solution is freeze-dried to obtain an amino sugar product.

[0029] References include: Ghadban A , Albertin L ,Rédéo W. Moussavou Mounguengui,etal.Synthesis of β- d -glucopyranuronosylamine in aqueous solution: kineticstudy and synthetic potential[J].Carbohydrate Research, 2011, 346(15):2384-2393.DOI:10.1016 / j.carres.2011.08.018. Likhosherstov LM , Novikova OS , Shibaev VN .New EfficientSynthesis of β-Glucosylamines of Mono and Disaccharides with the Use ofAmmonium Carbamate[J].Doklady Chemistry, 2002, 383(4-6):89-92.DOI:10.1023 / A:1015428720733. The preparation method provided in the embodiment of the present application performs glycosylation modification on the fiber surface on the basis of conventional nano-antibacterial fibers, thereby achieving a balance between the biosafety and antibacterial activity of the nano-antibacterial fibers, and utilizing the specific recognition of characteristic sugar chains and bacterial surface lectins to achieve active adhesion and enrichment of bacteria by the fiber material, thereby overcoming the spatial limitation problem existing in the physical antibacterial process, greatly improving the killing efficiency of antibacterial fibers on dispersed pathogenic microorganisms in the environment, and being suitable for preparing antibacterial dressings with active bacterial targeting.

[0030] The embodiment of the present application provides a method for preparing an impregnation liquid, comprising mixing compound I and compound III, adding a solvent to dissolve the reactants, stirring for a first reaction, and then adding compound II and continuing to stir for a second reaction to obtain the impregnation liquid.

[0031] In the preparation method of the embodiment of the present application, the amount of compound I, compound II, and compound III is calculated according to the degree of polymerization in formula (1). For example, when x is 80 and y is 20, compound I can be 100 mol, compound II can be 20 mol, and compound III can be 80 mol. Of course, those skilled in the art can appropriately adjust the amount according to the actual conversion rate, which will not be elaborated here.

[0032] In the preparation method of the embodiment of the present application, the solvent is dimethyl sulfoxide, and a buffer solution can be added at the same time to maintain the pH of the experimental system. In the first reaction, the reaction temperature can be 10-50°C, such as 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C, the reaction time can be 0.5~5 hours, such as 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, 4.5h, 5h, and the reaction pH can be 8~10, such as 8, 8.5, 9, 9.5, 10. In the second reaction, the reaction temperature can be 10-50°C, such as 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C; the reaction time can be 0.5~5 hours, such as 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, 4.5h, 5h; and the reaction pH can be 8~10, such as 8, 8.5, 9, 9.5, 10.

[0033] The embodiment of the present application provides an antibacterial fiber / fabric and a preparation method thereof, which is formed by impregnating the nano antibacterial fiber / fabric in the above-mentioned impregnation liquid for more than 6 hours, such as 6h, 12h, 18h, 24h, etc. The optional nano antibacterial fiber is nano silver antibacterial fiber, nano gold antibacterial fiber or graphene antibacterial fiber, such as nano silver modified antibacterial fiber, graphene modified nylon, graphene modified PET fiber, and the optional nano antibacterial fabric is woven or blended by the above fibers.

[0034] The antibacterial fabric can be used to prepare bacteria-active targeted antibacterial dressings. For example, the antibacterial fiber / fabric as described above is placed together with non-woven fabric, release paper, etc. on an applicator, and after cutting, pressing, etc., a dressing of the required specifications is made, and the antibacterial dressing product is obtained after packaging and disinfection.

[0035] Example 1 The preparation of the impregnation solution is divided into two steps: 1. Synthesis of side chain sugar-containing polymers The synthesis process is shown in reaction formula (1): Reaction (1) 0.1 mol of the reactive main chain polymer with a polymerization degree of 100 (NMR hydrogen spectrum (DMSO-d6) see Figure 1 ) was mixed with 6 mol 1-amino sugar (lactose). Then, DMSO was added to dissolve the reactants, the reaction pH was 8.5-9.5, stirred at room temperature for 1 hour, dialyzed and freeze-dried to obtain a side chain sugar-containing polymer.

[0036] The NMR spectra of side chain sugar polymers are as follows Figure 2 Shown (NMR hydrogen spectrum (D2O) of sugar chain polymer containing lactose structure in the side chain).

[0037] 2. Preparation of impregnation solution The synthesis process is shown in reaction formula (2): Reaction (2) First, the side chain sugar-containing polymer prepared in the previous step was redissolved, and then 4 mol of dopamine was added under a nitrogen atmosphere. After reacting at room temperature for 1 to 2 hours, an impregnation solution was obtained.

[0038] Example 2 It should be noted that the synthesis of the side chain sugar-containing polymer and the preparation of the impregnation solution in Example 1 are only for the convenience of structural characterization in each step. Example 2 of the present application can also use a one-pot reaction to perform glycosylation modification on the fiber surface, that is, after obtaining the reactive main chain polymer and amino sugar, the following reaction can be performed: The reactive main chain polymer (1.0 g) was dissolved in DMSO, and then 1.43 g of 1-aminolactose was added and stirred at room temperature for 1 hour under a nitrogen atmosphere. Then, 0.85 g of dopamine buffer (0.5 M, pH 9.0, 5 mL) was added and stirred for 2 hours under a nitrogen atmosphere until the reaction was complete.

[0039] Example 3 The graphene-modified PET fiber (6 mg / mL, Changzhou Henglibao Nano New Material Technology Co., Ltd.) was immersed in the impregnation solution of the above-mentioned Example 1 and allowed to stand for 12 to 24 hours. After the surface modification was completed, the fiber material was taken out, rinsed with deionized water, and vacuum dried to obtain the antibacterial fiber of Example 3 of the present application.

[0040] In order to verify that the sugar chain polymer was successfully attached to the nano antibacterial fiber, based on the specific binding between the sugar chain and the lectin protein, the antibacterial fiber (5 mg) of Example 3 was placed in a PBS solution (0.02 mg / mL) of concanavalin A lectin (ConA) labeled with fluorescein isothiocyanate (FITC) and incubated for 10 minutes. The material was then washed with PBS and observed under a confocal laser microscope (as shown in FIG. Figure 3 As shown in the figure), green fluorescence was observed on the fiber surface, proving that the sugar chain polymer was successfully attached to the graphene fiber surface.

[0041] The antibacterial properties of the original graphene fiber material (Native Fiber, i.e., the unimpregnated graphene-modified PET fiber in Example 3) and the glycosylated graphene fiber material (Glyco-fiber, lactose (D-Lactose) type, i.e., the antibacterial fiber impregnated in Example 3) were evaluated through in vitro antibacterial experiments and a mouse wound treatment model.

[0042] Evaluation principle: The in vitro antibacterial test was evaluated according to the provisions of GB / T 20944.3-2008 “Evaluation of antibacterial properties of textiles-Part 3 Oscillation method”.

[0043] First, Pseudomonas aeruginosa liquid was injected into the wound site of healthy mice to construct a bacterial infection wound model. The wound was then treated with graphene fiber fabric, and photos of the wound were taken every day to compare the wound treatment effects of different dressings.

[0044] The three bacteria used in this experiment are all antibiotic-resistant strains, and their specific information is shown in Table 2.

[0045] Table 2 Detailed information of drug-resistant strains

[0046] Experimental methods: 1. Take the fifth generation of bacterial culture, streak it on LB nutrient agar plate, culture it under suitable culture conditions for 24 hours, pick a typical colony from the plate with an inoculation loop, inoculate it into 20 mL of LB nutrient broth medium, and culture it under suitable conditions with shaking for 24 hours to prepare a bacterial suspension.

[0047] 2. Four-step dilution method: take 1 mL of bacterial suspension + 9 mL of LB nutrient broth medium; then take 1 mL of diluted bacterial suspension + 9 mL of LB nutrient broth medium; then take 1 mL of diluted bacterial suspension + 9 mL of PBS; then take 5 mL of diluted bacterial suspension + 45 mL of PBS to prepare the inoculum solution.

[0048] 3. Take 500 μL and 50 μL of inoculated bacterial solution respectively and add them to conical flasks containing 70 mL PBS. Set up four experimental groups (see Table 3): blank control, positive control, sample 1 (native fiber), sample 2 (Glyco-fiber, lactose (D-Lactose) type), set up two parallel controls for each group of experiments, and a total of 16 conical flasks for one experimental bacterium. Samples 1 and 2 were irradiated with UV for 10 minutes before being added to the conical flasks.

[0049] Table 3 Experimental group settings

[0050] 4. After sample preparation, shake for 30 min under appropriate culture conditions. After shaking, take 1 mL of the sample liquid and mix it with 15 mL of 50°C LB nutrient agar medium. Pour two plates into each conical flask, for a total of 32 plates for one experimental bacteria. After the LB nutrient agar medium in the plate solidifies, culture it under appropriate culture conditions for 24 hours. After the culture is completed, count the colonies.

[0051] Experimental results: The results of in vitro antibacterial experiments were as follows Figure 4 shown.

[0052] 1. Native Fiber exhibits antibacterial effects against three drug-resistant strains, with the inhibition rates being: Klebsiella pneumoniae (KP, 65%); Pseudomonas aeruginosa (PA, 70%); Proteus (PV, 50%).

[0053] 2. Due to the capture and enrichment effect of sugar chains on pathogenic microorganisms, glycosylated graphene fibers (Glyco-fiber) showed better antibacterial effects, with the inhibition rates being: Klebsiella pneumoniae (KP, 90%); Pseudomonas aeruginosa (PA, 93%); Proteus (PV, 70%).

[0054] 3. Both the original fiber and the glycosylated modified fiber need to be excited by ultraviolet light, that is, they must first be irradiated under a 254nm ultraviolet lamp for 10 minutes before the subsequent antibacterial test. Graphene materials need to be under light excitation conditions to catalyze water molecules to generate highly active hydroxyl free radicals, thereby destroying the cell membrane structure of pathogenic microorganisms and achieving antibacterial effects.

[0055] The results of the mouse wound model treatment experiment are shown in Figure 5 .

[0056] The mouse wound model infected with antibiotic-resistant Pseudomonas aeruginosa was treated with graphene fiber fabric (Native Fiber) and glycosylated graphene fiber fabric (Glyco-Fiber). The control group (Control) was treated with ordinary gauze soaked in tetracycline. The wound dressing was changed once a day, and the dressing was irradiated with ultraviolet light for 10 minutes before covering the mouse wound. It should be clearly pointed out here that ultraviolet light irradiation is only used to excite the material, not to irradiate the wound site with ultraviolet light. Through 7 consecutive days of observation, it can be seen that both graphene fiber fabrics showed an inhibitory effect on Pseudomonas aeruginosa infection, and the treatment effect of the glycosylated group (Glyco-Fiber) was better than that of the original fiber fabric group.

[0057] The results of the cytotoxicity and bacterial enrichment experiments of graphene fiber materials are shown in Figure 6 .

[0058] The cytotoxicity of the two fibers was evaluated using COS-7 mammalian cells. Figure 6As shown in (A), both the original graphene fiber and the glycosylated graphene fiber showed good cell compatibility. Microscopic photos showed that cells could attach to the fiber surface and grow, which showed that both fibers had no cytotoxicity. In addition, the two fibers were co-incubated with bacterial culture fluid, and observation under the microscope showed that ( Figure 6 (B) : After glycosylation modification, bacteria are obviously enriched near the fibers.

[0059] Experimental conclusion: Both in-situ polymerized graphene fibers and glycosylated graphene fibers have antibacterial activity, but glycosylation modification can significantly improve the antibacterial properties of original graphene fibers. Graphene fibers are non-cytotoxic, and glycosylation modification can promote the enrichment of pathogenic microorganisms on the fiber surface.

[0060] Fiber surface glycosylation modification bonding strength experiment: The glycosylation-modified graphene PET fiber fabric (lactose (D-Lactose) type) was washed continuously, and the surface XPS analysis of the fabric after 12 hours and 24 hours of washing was performed. The results are as follows Figure 7 As shown in the figure, after continuous water washing for 12 hours, the glycosylation retention rate was 86%, and after continuous water washing for 24 hours, the glycosylation modification retention rate was 55%. The above results show that the modification method can obtain a firmly bonded glycosylated coating on the surface of graphene fibers.

[0061] In the above embodiments, graphene PET fiber is used as the matrix, and lactose is used as the characteristic sugar chain, and the ideal effect is achieved. The applicant further uses nano-silver antibacterial fiber and nano-gold antibacterial fiber as the matrix, and selects glucose, mannose, maltose, galactose, etc. as the characteristic sugar chain, and achieves the ideal effect similar to the above embodiments, which will not be repeated here.

[0062] The above describes in detail the structure, features and effects of the present application based on the embodiments shown in the drawings. The above is only a preferred embodiment of the present application, but the present application does not limit the scope of implementation to what is shown in the drawings. Any changes made in accordance with the concept of the present application, or modifications to equivalent embodiments with equivalent changes, which still do not exceed the spirit covered by the description and drawings, should be within the protection scope of the present application.

Claims

1. An impregnation liquid, characterized in that The impregnation solution includes a component having a structural formula as shown in formula (1): Formula (1) In formula (1), x and y are independently selected from integers between 1 and 100, and n1 is selected from integers between 1 and 20; R1 is a hydrogen atom or a hydrocarbon group, and R2 is a hydrogen atom, a hydrocarbon group, an amino group, a hydroxyl group, or an atom or an atomic group that can replace the above groups.

2. The impregnation liquid according to claim 1, characterized in that In formula (1), x is 50~80, and y is 20~50.

3. A method for preparing an impregnation solution, characterized in that: Compound I, compound II, and compound III are mixed and reacted to obtain an impregnation solution; The structural formula of the compound I is shown in formula (2): Formula (2) In formula (2), m is an integer between 50 and 150; The structural formula of the compound II is shown in formula (3): Formula (3); In formula (3), R3 is a hydrogen atom or a hydrocarbon group, and R4 is a hydrogen atom, a hydrocarbon group, an amino group, a hydroxyl group, or an atom or an atomic group that can replace the above groups; The structural formula of the compound III is shown in formula (4) or formula (5): Formula (4), Formula (5) In formula (4), n2 is an integer between 1 and 20; In formula (5), n2 and n3 are independently selected from integers between 1 and 20, and P is an oxygen atom, a carbon atom, a nitrogen atom, or a sulfur atom.

4. The preparation method according to claim 3, characterized in that: The compound III is amino-modified glucose, mannose, maltose, galactose or lactose.

5. The preparation method according to claim 3, characterized in that: Compound I and compound III are mixed, a solvent is added to dissolve the reactants, and the mixture is stirred for a first reaction, and then compound II is added and stirred for a second reaction to obtain the impregnation solution.

6. The preparation method according to claim 5, characterized in that: The conditions of the first reaction are reaction temperature of 10-50° C., reaction time of 0.5-5 hours, and reaction pH of 8-10.

7. The preparation method according to claim 5, characterized in that: The conditions of the second reaction are reaction temperature of 10-50° C., reaction time of 0.5-5 hours, and reaction pH of 8-10.

8. Antibacterial fiber, characterized in that: The impregnation liquid according to claim 1 is used to impregnate the nano antibacterial fiber, and the nano antibacterial fiber is nano silver antibacterial fiber, nano gold antibacterial fiber or graphene antibacterial fiber.

9. Antibacterial fabric, characterized in that The antibacterial fiber is woven from the nano-antibacterial fiber impregnated with the impregnation solution described in claim 1, or the nano-antibacterial fabric is impregnated with the impregnation solution described in claim 1; the nano-antibacterial fiber is nano-silver antibacterial fiber, nano-gold antibacterial fiber or graphene antibacterial fiber, and the nano-antibacterial fabric is nano-silver antibacterial fabric, nano-gold antibacterial fabric or graphene antibacterial fabric.

10. Use of the antibacterial fabric according to claim 9 in preparing antibacterial dressings.

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