High-performance radioactivity detection microwell plate, preparation method thereof and application of high-performance radioactivity detection microwell plate in detection of 14C labeled compound

Abstract

The invention discloses a high-performance radioactivity detection microwell plate and a preparation method and application thereof in detection of 14C labeled compounds, the high-performance radioactivity detection microwell plate comprises a microwell plate body and a scintillator reagent filled in micropores of the microwell plate body, the scintillator reagent is a mixture of a eutectic scintillator and a dispersing agent, and the eutectic scintillator is prepared from p-terphenyl: PTP and 1, 2-propylene glycol. The catalyst is prepared from the following raw materials: 1, 4-bis (2-methylstyryl) benzene: Bis-MSB. The raw materials are easy to obtain, the preparation process is simple, and the cost is low and is only 20% of that of imported similar products; compared with a radioactive detection microporous plate sold in the market, the microporous plate disclosed by the invention is better in stability under a high-temperature condition, wider in linear range and wider in application scene; the microwell plate provided by the invention has good detection efficiency, stability and accuracy in the aspect of 14C radioactivity detection.

Description

Technical Field

[0001] The present invention relates to the field of radioactive label detection technology, and in particular to a high-performance radioactive detection microplate, a preparation method thereof and a method for 14 Application in the detection of C-labeled compounds. Background Art

[0002] In drug development, the main reliance 14 Radiopharmaceutical metabolism experiments using C-radiolabeled analogs require the use of radioactive detection techniques to determine the metabolite profiles in different biological matrices and determine the number of metabolites and the relative concentrations of each metabolite. Online radioflow detection (RFD) and offline liquid scintillation counting (LSC) have been routinely used for the analysis of radiolabeled metabolites. Online radioflow detection offers many advantages, including rapid analysis, high throughput, high resolution, accuracy, and precision. However, its major disadvantage is relatively low sensitivity due to the short retention time (5–15 s) of the radioactive chromatographic peak in the RFD detection cell. This significantly limits its application in the analysis of low-concentration radioactive metabolites, such as those in plasma. Offline liquid scintillation counting (LSC) offers at least an order of magnitude greater sensitivity than RFD, as individual fractions can be counted for extended periods (typically 10 minutes or longer). Therefore, LSC has traditionally been used for the quantitative analysis of low-concentration radiolabeled metabolites. However, LSC can only count the radioactivity of one fraction at a time. The entire process is time-consuming and labor-intensive, and it consumes scintillation fluid, generating large amounts of radioactive waste that is difficult to handle. Furthermore, because fractions are collected in time segments, LSC's peak resolution is inferior to other techniques. Consequently, LSC has rarely been used in metabolite profiling.

[0003] Microplate scintillation counting (MSC) is an offline liquid radiochromatographic detection technology introduced in recent years for radioactive metabolite spectrum analysis. 14When beta rays emitted by a C nuclide pass through a scintillator, the scintillator is ionized and excited by the radiation, emitting light of a specific wavelength. These photons strike the photocathode of a photomultiplier tube, generating a photoelectric effect that releases electrons. These electrons flow through the multi-stage cathode circuit of the electromultiplier tube, where they are amplified step by step or as electrical pulses, which are input into the electronic circuitry and then recorded by a calibrator. Compared to LSC, MSC not only increases analytical throughput and sensitivity but also reduces radioactive waste and manual handling of LSC vials. In some metabolic research laboratories, MSC has become the method of choice for analyzing low-concentration radioactive metabolites. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to provide a high performance radioactivity detection microplate, a preparation method thereof and a method for 14 Application in the detection of C-labeled compounds.

[0007] In order to solve the above technical problems, the technical solution adopted by the present invention is: the first aspect of the present invention provides a high-performance radioactivity detection microplate, including a microplate body and a scintillator reagent filled in the micropores of the microplate body, the scintillator reagent is a mixture of a eutectic scintillator and a dispersant, and the eutectic scintillator is prepared from raw materials including p-terphenyl: PTP and 1,4-bis(2-methylphenyl)benzene: Bis-MSB.

[0008] Preferably, the dispersant is polyvinyl pyrrolidone.

[0009] A second aspect of the present invention provides a method for preparing the high-performance radioactivity detection microplate as described above, comprising the following steps:

[0010] S1. Preparation of eutectic scintillator:

[0011] PTP and Bis-MSB are mixed in p-xylene, stirred under heating, then cooled to room temperature, filtered, the solid is washed, and vacuum dried to obtain the eutectic scintillator;

[0012] S2. Preparing a suspension of eutectic scintillator and dispersant:

[0013] S2-1, taking a dispersant polyvinyl pyrrolidone and adding it to ethanol, and ultrasonicating until the polyvinyl pyrrolidone is completely dissolved to obtain a dispersant solution;

[0014] S2-2, adding the eutectic scintillator prepared in step S1 to the dispersant solution prepared in step S2-1, stirring evenly and then ultrasonically dispersing to obtain a suspension of the eutectic scintillator and the dispersant;

[0015] S3. Add the suspension of the eutectic scintillator and the dispersant obtained in step S2 into the micropores of the microplate body, let the microplate body stand at room temperature, heat and dry the solvent, and cool to room temperature to obtain the high-performance radioactivity detection microplate.

[0016] Preferably, step S1 is specifically:

[0017] PTP and Bis-MSB are mixed in p-xylene at a mass ratio of PTP:Bis-MSB = 50:1 to 5:1, and the ratio of the total mass of PTP and Bis-MSB to the volume of p-xylene is controlled to be 0.1 to 0.3 mg / mL. The mixture is stirred at 110-150° C. for 15-60 minutes, and then the mixture is cooled to room temperature and filtered. The solid is washed with p-xylene and ethanol in sequence, and vacuum dried at 50-70° C. to constant weight to obtain the eutectic scintillator.

[0018] Preferably, step S2 is specifically:

[0019] S2-1, taking a dispersant polyvinyl pyrrolidone and adding it to ethanol, sonicating until the polyvinyl pyrrolidone is completely dissolved, to prepare a dispersant solution with a polyvinyl pyrrolidone concentration of 2 to 20 mg / mL;

[0020] S2-2, taking the eutectic scintillator prepared in step S1 and adding it to the dispersant solution prepared in step S2-1, controlling the concentration of the eutectic scintillator to be 0.05-0.2 g / mL, stirring evenly and then ultrasonically dispersing for 5-20 minutes to obtain a suspension of the eutectic scintillator and the dispersant;

[0021] Preferably, in step S1, the mass ratio of PTP:Bis-MSB is 20:1; and in step S2, the concentration of polyvinyl pyrrolidone in the dispersant solution is 10 mg / mL.

[0022] Preferably, step S3 is specifically:

[0023] The suspension of the eutectic scintillator and the dispersant obtained in step S2 is added to the micropores of the microplate body. The suspension is stirred during the addition process, and the volume of the suspension added to each micropore is controlled to be 20% to 80% of the micropore volume. The microplate body is then allowed to stand at room temperature for 15-60 minutes, then heated at 50-70°C for 0.5-2 hours to dry the solvent, and cooled to room temperature to obtain the high-performance radioactivity detection microplate.

[0024] Preferably, the method for preparing the high-performance radioactivity detection microplate comprises the following steps:

[0025] S1. Preparation of eutectic scintillator:

[0026] PTP and Bis-MSB were mixed in p-xylene at a mass ratio of PTP:Bis-MSB = 20:1, and the total mass of PTP and Bis-MSB to the volume of xylene was controlled to be 0.2 mg / mL. The mixture was magnetically stirred at 135°C for 30 minutes, and then the mixture was cooled to room temperature at a rate of 5°C / min and filtered. The solid was washed with p-xylene and ethanol in sequence, and dried in vacuo at 60°C to constant weight to obtain the eutectic scintillator.

[0027] S2. Preparing a suspension of eutectic scintillator and dispersant:

[0028] S2-1. Add polyvinyl pyrrolidone (a dispersant) to ethanol and sonicate until the polyvinyl pyrrolidone is completely dissolved to prepare a dispersant solution having a polyvinyl pyrrolidone concentration of 10 mg / mL.

[0029] S2-2, taking the eutectic scintillator prepared in step S1 and adding it to the dispersant solution prepared in step S2-1, controlling the concentration of the eutectic scintillator to be 0.1 g / mL, stirring evenly and then ultrasonically dispersing for 10 minutes to obtain a suspension of the eutectic scintillator and the dispersant;

[0030] S3. Add the suspension of the eutectic scintillator and the dispersant obtained in step S2 into the micropores of the microplate body, keep stirring the suspension during the addition process, and control the volume of the suspension added to each micropore to be 1 / 3 of the micropore volume; then let the microplate body stand at room temperature for 30 minutes, then heat it at 60°C for 1 hour to dry the solvent, and cool it to room temperature to obtain the high-performance radioactivity detection microplate.

[0031] Preferably, in step S3, the micropore volume of the micropore plate body is 300 μL, and the volume of the suspension added to each micropore is 100 μL.

[0032] The third aspect of the present invention provides a high performance radioactivity detection microplate as described above. 14 Application in the detection of C-labeled compounds.

[0033] Preferably, when using the microplate for detection, the sample is added to the microplate using a pipette, or the sample is collected into the microplate according to a time period (generally 10 seconds) using an HPLC-fraction collection system. The microplate containing the sample is then dried at room temperature, heated and dried, or centrifuged to remove the solvent, and then the radioactivity is measured using a MicroBeta2 microplate counter.

[0034] The beneficial effects of the present invention are:

[0035] The present invention provides a high performance radioactivity detection microplate, a preparation method thereof and its application in 14The present invention uses p-terphenyl (PTP) as the first scintillator, 1,4-bis(2-methylphenyl)benzene as a wave shifter, polyvinylpyrrolidone as a dispersant, and a polystyrene 96-well plate as a carrier. By preparing the eutectic scintillator and performing a simple one-step filling and drying, a microplate for β-ray radioactivity determination can be obtained.

[0036] The raw materials of the present invention are easily available, the preparation process is simple, and the cost is low, which is only 20% of the imported similar products;

[0037] Compared with commercially available radioactivity detection microplates, the microplate of the present invention has better stability under high temperature conditions, a wider linear range, and a wider range of application scenarios;

[0038] The microplate of the present invention is 14 C radioactivity detection has good detection efficiency, stability and accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 Schematic diagram of a high-performance radioactivity detection microplate of the present invention;

[0040] Figure 2 The results of the experiment to optimize the amount of suspension added to each well of the microplate;

[0041] Figure 3 To conduct experiments to optimize the amount of dispersant added;

[0042] Figure 4 Deepwell LumaPlate TM -Linear relationship of 96 microplates;

[0043] Figure 5 is the linear relationship of the homemade microplate;

[0044] Figure 6 Deepwell LumaPlate TM - 96-well microplate sample test results;

[0045] Figure 7 These are the test results of the homemade microplate samples.

[0046] Description of reference numerals:

[0047] 1—microporous plate; 2—micropore; 3—eutectic scintillator and dispersant; 4— 14 C labeled compound. DETAILED DESCRIPTION

[0048] The present invention is further described in detail below with reference to the embodiments so that those skilled in the art can implement the invention with reference to the description.

[0049] It should be understood that terms such as “having”, “including” and “comprising” used herein do not preclude the existence or addition of one or more other elements or combinations thereof.

[0050] Unless otherwise specified, the experimental methods used in the following examples are conventional methods. Materials and reagents used in the following examples are commercially available unless otherwise specified. In the following examples, where specific conditions are not specified, the experiments were conducted under conventional conditions or those recommended by the manufacturer. Reagents and instruments used, where the manufacturer is not specified, are commercially available conventional products.

[0051] Example 1

[0052] This embodiment provides a high-performance radioactivity detection microplate, the preparation method of which includes the following steps:

[0053] S1. Preparation of eutectic scintillator:

[0054] PTP (p-terphenyl) and Bis-MSB (1,4-bis(2-methylphenylvinyl)) were mixed in p-xylene at different mass ratios of PTP:Bis-MSB = 50:1 to 5:1, and the total mass of PTP and Bis-MSB to the volume of xylene was controlled to be 0.2 mg / mL. The mixture was magnetically stirred at 135°C for 30 minutes, and then the mixture was cooled to room temperature at a rate of 5°C / min and filtered. The solid was washed with p-xylene and ethanol in sequence, and dried in vacuo at 60°C to constant weight to obtain a eutectic scintillator. The eutectic scintillator was ground and used for later use.

[0055] S2. Preparing a suspension of eutectic scintillator and dispersant:

[0056] S2-1. Add polyvinyl pyrrolidone (a dispersant) to ethanol and sonicate until the polyvinyl pyrrolidone is completely dissolved to prepare a dispersant solution having a polyvinyl pyrrolidone concentration of 10 mg / mL.

[0057] S2-2, taking the eutectic scintillator prepared in step S1 and adding it to the dispersant solution prepared in step S2-1, controlling the concentration of the eutectic scintillator to be 0.1 g / mL, stirring evenly and then ultrasonically dispersing for 10 minutes to obtain a suspension of the eutectic scintillator and the dispersant;

[0058] S3. Add 100 μL of the suspension of the eutectic scintillator and dispersant obtained in step S2 to the microwells of the microwell plate body (white polystyrene 96 microwell plate, microwell volume is 300 μL), and keep stirring the suspension during the addition process (to prevent the eutectic scintillator from settling); then let the microwell plate body stand at room temperature for 30 minutes, then heat it at 60°C for 1 hour to dry the solvent, and cool it to room temperature to obtain a high-performance radioactivity detection microwell plate (hereinafter referred to as the homemade microwell plate).

[0059] Reference Figure 1 , which is a schematic diagram of a high-performance radioactive detection microplate, including a eutectic scintillator and a dispersant 3 filled in the micropores 2 of the microplate body 1. 14 A sample of C-labeled compound 4 was added thereto.

[0060] The sample collection and measurement methods are as follows:

[0061] The sample to be tested was collected into a microplate according to a time period (10 s) using an HPLC-fraction collection system. The microplate containing the sample was then heated and dried to remove the solvent, and radioactivity was determined using a MicroBeta2 microplate counter.

[0062] The following uses this method to conduct relevant test experiments based on Example 1.

[0063] Test Example 1 Comparison of measurement results of different scintillator combinations

[0064] In this example, according to the preparation method of Example 1, a homemade microplate was used to measure the different combinations of the first scintillator and the wave shifter (the mass ratio of the two was 10:1, the concentration of the eutectic scintillator in the suspension was 0.1 g / mL, and the rest was the same as Example 1), and equal amounts of 14 Response after C-labeling of compounds.

[0065] The test results are shown in Table 1 below:

[0066] Table 1

[0067]

[0068]

[0069] Note: PPO, 2,5-diphenyloxazole; POPOP, 1,4-bis(5-phenyl-2-oxazolyl)benzene.

[0070] Conclusion: The test results show that under the same conditions, the scintillator combination of PTP and Bis-MSB has higher count values, higher detection efficiency and better repeatability.

[0071] Test Example 2 Optimization experiment of the amount of suspension added to each well of the microplate

[0072] After determining the scintillator combination of PTP and Bis-MSB (the mass ratio of the two was 10:1, and the scintillator concentration was 0.1 g / mL), the optimal volume of the suspension added to each well during the preparation of the microplate was further explored according to the preparation method of Example 1 (only the volume of the suspension added in the microwell was changed).

[0073] The test results are as follows Figure 2 The results show that the MSC count results are similar when the volume of suspension added to each well ranges from 50 to 150 μL. For cost and ease of use, the volume of scintillant suspension added to each well was selected as 100 μL / well in subsequent experiments.

[0074] Test Example 3: First scintillator and wave shifter quality optimization experiment

[0075] Based on the above experimental results, the optimal mass ratio of PTP and Bis-MSB was further explored. According to the method of Example 1, only the mass ratio of PTP and Bis-MSB was changed. The test results are shown in Table 2 below:

[0076] Table 2

[0077]

[0078]

[0079] Conclusion: Test results show that MSC counts are similar when the mass ratio of PTP to Bis-MSB is within the range of 50:1 to 5:1. For cost and counting stability considerations, a mass ratio of 20:1 was selected for subsequent experiments.

[0080] Test Example 4: Dispersant Dosage Optimization Experiment

[0081] This test example also explored the amount of polyvinyl pyrrolidone (PVP) added as a dispersant in the scintillator suspension. The method of Example 1 was followed, with only the amount of PVP added being changed.

[0082] The test results are as follows Figure 3 As shown, it can be seen that with the increase in the amount of dispersant added, the counting results show a trend of first increasing and then decreasing. When the amount of polyvinyl pyrrolidone added is 10 mg / mL, the detection efficiency is relatively the highest, and it can also exert the greatest anti-sedimentation effect.

[0083] Test Example 5: Homemade Microplate and Commercially Available PerkinElmer Deepwell LumaPlate TM -96 microplate comparison

[0084] Ⅰ. Temperature Stability Comparison

[0085] Since the softening point of the scintillator used in commercial microplates is generally 75°C, the allowable operating temperature range is usually -60 to 60°C, and the efficiency will decrease under high temperature conditions. Therefore, we added an equal amount of 14 C-labeled compounds were dried at different temperatures to compare the Deepwell LumaPlate TM-The performance of the 96-well microplate and the homemade microplate of Example 1 at different temperatures. The test results are shown in Table 3 below:

[0086] Table 3

[0087]

[0088]

[0089] Conclusion: The test results show that Deepwell LumaPlate TM The detection efficiency of the -96 microplate showed temperature dependence, with measured values ​​decreasing by 5% at 60°C and a 32% decrease at 75°C. However, the measured values ​​of the homemade microplate remained stable even at 75°C, showing no decrease. This may be due to the fact that commercially available microplates use a polymer matrix such as polystyrene or polymethylstyrene to achieve a uniform mixing of the first scintillator and the photon shifter. However, these polymers generally have low softening points, and higher operating temperatures can cause changes in the polymer structure, restricting the transfer of photons between the matrix and the scintillator, leading to a decrease in detection efficiency. The homemade microplate, by creating a eutectic material of the first scintillator and the photon shifter, facilitates the transfer of photons between the scintillators while avoiding the use of a polymer matrix, ensuring the microplate's performance at high temperatures.

[0090] II. Comparison of linear range

[0091] To determine the number of metabolites and the relative concentration of each metabolite using MSC technology, the MSC count results must be consistent with the 14 The content of C-labeled compounds has a linear relationship. Therefore, this test case explored the difference between the homemade microplate and the commercially available Deepwell LumaPlate. TM -Linear range of 96-well microplate under high temperature conditions (75°C).

[0092] The test results are as follows Figure 4 (Deepwell LumaPlate TM -96 microplate) and Figure 5 (Linear relationship of homemade microplate) As shown in the test results, the commercially available Deepwell LumaPlate TMThe 96-well microplate exhibited a linear relationship only within the activity range of 0 to 6000 CPM. In contrast, the homemade microplate exhibited a linear relationship across the activity range of 0 to 11000 CPM and even had the potential to exhibit a linear range over a wider activity range. Therefore, the microplate prepared in this invention has broader application prospects and can change the current situation where MSCs are limited to analyzing low-concentration radioactive metabolites.

[0093] III. Comparison of actual samples

[0094] To further compare the homemade microplate with the commercially available Deepwell LumaPlate TM -96 microplate performance in actual applications. In this test example, the same processed clinical radioactive sample (fecal matrix) was collected in two microplates using an HPLC-fraction collection system. After centrifugation and concentration to remove the solvent (heating temperature 60°C), the sample was measured using a MicroBeta2 microplate reader.

[0095] The test results are as follows Figure 6 (Deepwell LumaPlate TM -96 microplates) and Figure 7 (self-made microplate) as shown, the test results show that the self-made microplate and Deepwell LumaPlate TM The -96 microplate had a high degree of consistency, detecting 13 metabolites, and the peak area percentage of each metabolite was not much different, indicating that the homemade microplate can be used for radioactive metabolite analysis experiments.

[0096] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the description and implementation methods. They can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to specific details.

Claims

1. A high performance radioactivity detection microplate, characterized in that: The invention comprises a microplate body and a scintillator reagent filled in the micropores of the microplate body. The scintillator reagent is a mixture of a eutectic scintillator and a dispersant. The eutectic scintillator is prepared from raw materials including p-terphenyl: PTP and 1,4-bis(2-methylphenylvinyl)benzene: Bis-MSB.

2. The method for preparing a high-performance radioactivity detection microplate according to claim 1, characterized in that: The dispersant is polyvinyl pyrrolidone.

3. A method for preparing a high-performance radioactivity detection microplate as claimed in claim 1 or 2, characterized in that: The following steps are involved: S1. Preparation of eutectic scintillator: PTP and Bis-MSB are mixed in p-xylene, stirred under heating, then cooled to room temperature, filtered, solid washed, and vacuum dried to obtain the eutectic scintillator; S2. Preparing a suspension of eutectic scintillator and dispersant: S2-1, taking a dispersant polyvinyl pyrrolidone and adding it to ethanol, and ultrasonicating until the polyvinyl pyrrolidone is completely dissolved to obtain a dispersant solution; S2-2, adding the eutectic scintillator prepared in step S1 to the dispersant solution prepared in step S2-1, stirring evenly and then dispersing by ultrasonication to obtain a suspension of the eutectic scintillator and the dispersant; S3, adding the suspension of the eutectic scintillator and the dispersant obtained in step S2 into the micropores of the microplate body, leaving the microplate body at room temperature, heating and drying the solvent, and cooling to room temperature to obtain the high-performance radioactivity detection microplate.

4. The method for preparing a high-performance radioactivity detection microplate according to claim 3, characterized in that: Step S1 is specifically as follows: According to the mass ratio of PTP:Bis-MSB=50:1-5:1, PTP and Bis-MSB are mixed in p-xylene, and the ratio of the total mass of PTP and Bis-MSB to the volume of p-xylene is controlled to be 0.1-0.3 mg / mL, and the mixture is stirred at 110-150° C. for 15-60 min. Stirring is stopped, and the mixture is cooled to room temperature and then filtered. The solid is washed with p-xylene and ethanol in sequence, and vacuum dried at 50-70° C. to constant weight to obtain the eutectic scintillator.

5. The method for preparing a high-performance radioactivity detection microplate according to claim 4, characterized in that: Step S2 is specifically as follows: S2-1, taking a dispersant polyvinyl pyrrolidone and adding it to ethanol, ultrasonicating until the polyvinyl pyrrolidone is completely dissolved, and preparing a dispersant solution with a polyvinyl pyrrolidone concentration of 2 to 20 mg / mL; S2-2, take the eutectic scintillator prepared in step S1 and add it to the dispersant solution prepared in step S2-1, control the concentration of the eutectic scintillator to be 0.05-0.2 g / mL, stir evenly and then ultrasonically disperse for 5-20 minutes to obtain a suspension of the eutectic scintillator and the dispersant.

6. The method for preparing a high-performance radioactivity detection microplate according to claim 5, characterized in that: In step S1, the mass ratio of PTP:Bis-MSB is 20:1; in step S2, the concentration of polyvinyl pyrrolidone in the dispersant solution is 10 mg / mL.

7. The method for preparing a high-performance radioactivity detection microplate according to claim 3, characterized in that: Step S3 is specifically as follows: Add the suspension of the eutectic scintillator and the dispersant obtained in step S2 into the micropores of the microplate body, keep stirring the suspension during the addition process, and control the volume of the suspension added to each micropore to be 20% to 80% of the volume of the micropore; then let the microplate body stand at room temperature for 15-60 minutes, then heat at 50-70°C for 0.5-2h to dry the solvent, and cool to room temperature to obtain the high-performance radioactivity detection microplate.

8. The method for preparing a high-performance radioactivity detection microplate according to claim 3, characterized in that: The following steps are involved: S1. Preparation of eutectic scintillator: PTP and Bis-MSB were mixed in p-xylene at a mass ratio of PTP:Bis-MSB=20:1, the total mass of PTP and Bis-MSB was controlled to have a ratio of 0.2 mg / mL to the volume of xylene, and the mixture was magnetically stirred at 135°C for 30 min, the stirring was stopped, the mixture was cooled to room temperature at a cooling rate of 5°C / min, and then filtered, the solid was washed with p-xylene and ethanol in sequence, and vacuum dried at 60°C to constant weight to obtain the eutectic scintillator; S2. Preparing a suspension of eutectic scintillator and dispersant: S2-1, taking a dispersant polyvinyl pyrrolidone and adding it to ethanol, ultrasonicating until the polyvinyl pyrrolidone is completely dissolved, and preparing a dispersant solution with a polyvinyl pyrrolidone concentration of 10 mg / mL; S2-2, adding the eutectic scintillator prepared in step S1 to the dispersant solution prepared in step S2-1, controlling the concentration of the eutectic scintillator to be 0.1 g / mL, stirring evenly and then ultrasonically dispersing for 10 minutes to obtain a suspension of the eutectic scintillator and the dispersant; S3. Add the suspension of the eutectic scintillator and the dispersant obtained in step S2 into the micropores of the microplate body, keep stirring the suspension during the addition process, and control the volume of the suspension added to each micropore to be 1 / 3 of the volume of the micropore; then let the microplate body stand at room temperature for 30 minutes, then heat it at 60°C for 1 hour to dry the solvent, and cool it to room temperature to obtain the high-performance radioactivity detection microplate.

9. The method for preparing a high-performance radioactivity detection microplate according to claim 8, characterized in that: In step S3, the microwell volume of the microwell plate body is 300 μL, and the volume of the suspension added to each microwell is 100 μL.

10. A high performance radioactive detection microplate as claimed in any one of claims 1 to 2 or a high performance radioactive detection microplate prepared by the method as claimed in any one of claims 3 to 9. 14 Application in the detection of C-labeled compounds.

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