Bactericidal composition containing feneptadoquin and application thereof

By using a combination technology of a bactericidal composition containing feneptamidoquin and fluzozolamide or radizolamide in the prevention and treatment of cucumber grey mold, the resistance problem is solved, the effective prevention and treatment effect of high-efficiency and low dose is achieved, and the generation of drug resistance is delayed.

CN119586622AActive Publication Date: 2025-03-11QINGDAO HAILIER BIOTECHNOLOGY CO LTD

Patent Information

Application Number
CN202411773969.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-05
Publication Date
2025-03-11
Estimated Expiration
2044-12-05

AI Technical Summary

Technical Problem

The prior art is prone to resistance problems when preventing and treating cucumber grey mold, which leads to an increase in the dosage of medicine and threatens environmental and ecological security.

Method used

The bactericidal composition containing feneptamidoquin is used to significantly enhance the bactericidal effect within a certain proportion range by combining it with feneptamidoquin or radisomide, and delay the generation of drug resistance of pathogenic bacteria.

Benefits of technology

It significantly improves the prevention and treatment effect of cucumber gray mold, reduces the use of medicine, extends the service life of medicine, and is environmentally friendly.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention belongs to the technical field of pesticide sterilization, and discloses a feneptadoquin-containing bactericidal composition and application thereof, the active component A is feneptadoquin, the active component B is any one of fluxapyroxad or boscalid, and the mass ratio of the active component A to the active component B is 1: 27-30: 1. The bactericidal composition disclosed by the invention has a good prevention and treatment effect, has a synergistic effect on prevention and treatment of fungal diseases of plants, can be used for effectively controlling pathogenic microorganisms to generate drug resistance and reducing the dosage, and is environment-friendly.
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Description

Technical Field

[0001] The invention relates to the field of pesticide compound compositions, and in particular to a fungicide composition containing feneptamidoquin and application thereof. Background Art

[0002] Cucumber gray mold is common in cucumber cultivation, seriously affecting the quality and yield of cucumbers. Botrytis cinerea is a common pathogen that causes cucumber gray mold. Cucumber gray mold can harm the flowers, leaves, melons and stems of cucumbers. Generally, it first invades from the male flowers that have withered, causing the flowers to rot and then produce a gray-brown mold layer. As the disease progresses, it begins to spread to young melons. The infected young melons gradually become water-stained and slowly begin to rot. Later, a large amount of gray-brown mold will be produced on the rotten parts.

[0003] Fluopyrab is a SDHI fungicide. Fluopyrab can be used for crops such as cereals, soybeans, corn, vegetables, fruits, and cash crops to control diseases through foliar or seed treatment. Fluopyrab is an inhibitor of succinate dehydrogenase in complex II of the mitochondrial respiratory chain, thereby inhibiting the germination of spores, germ tubes, and mycelium of the target fungus.

[0004] Boscalid is a nicotinamide fungicide, mainly used to control powdery mildew, gray mold, various rots, brown rot and root rot, etc. Boscalid is an inhibitor of succinate coenzyme Q reductase in the mitochondrial respiratory chain, has a strong inhibitory effect on spore germination, and has no cross-resistance with other fungicides.

[0005] The long-term use of a single chemical pesticide is very likely to cause resistance problems, resulting in a continuous increase in the dosage of the pesticide, an increase in the risk of resistance, and is not conducive to environmental ecological safety. Seeking a scientific and reasonable pesticide compound is a good way to solve this problem. After agent screening and indoor and field tests, the applicant conducted in-depth research on feneptamidoquin and flupyraclostrobin or boscalid and their compositions, and found that the compounding of feneptamidoquin with either flupyraclostrobin or boscalid has a significant synergistic effect within a certain proportion range, and there is a significant difference in the mechanism of action of feneptamidoquin and flupyraclostrobin or boscalid, which expands the application range of the agent, reduces the dosage, delays the generation and development of drug resistance in pathogenic bacteria, and is environmentally friendly. Summary of the invention

[0006] Based on the above situation, the purpose of the present invention is to provide a fungicide composition containing feneptamidoquin, which can be used to prevent and treat fungal diseases, especially cucumber gray mold, and has significant control effect. It can reduce the amount of medicine used and delay the generation and development of drug resistance of pathogenic bacteria, and is environmentally friendly.

[0007] In order to achieve the above object, the present invention adopts the following technical scheme: a fungicide composition containing feneptamidoquin, the fungicide composition comprises an active ingredient A and an active ingredient B, the active ingredient A is feneptamidoquin, the active ingredient B is any one of fluopyraclostrobin or boscalid, and the mass ratio of the active ingredient A to the active ingredient B is 1:27 to 30:1, or any value within the above numerical range.

[0008] Furthermore, the active ingredient B is fluopyrab, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1, or any value within the above numerical range;

[0009] The active ingredient B is boscalid, and the mass ratio of the active ingredient A to the active ingredient B is 1:27 to 21:1, or any value within the above numerical range.

[0010] Furthermore, the active ingredient B is fluopyrab, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 25:1, or any value within the above numerical range;

[0011] The active ingredient B is boscalid, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 12:1, or any value within the above numerical range;

[0012] Furthermore, the total weight of the bactericidal composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.1% to 80%, preferably 1% to 70% of the total weight of the bactericidal composition;

[0013] Furthermore, the fungicide composition includes, in addition to the active ingredient, an auxiliary ingredient permitted in agriculture, wherein the auxiliary ingredient is selected from one or more of a wetting agent, a dispersant, a thickener, a disintegrant, an emulsifier, a defoaming agent, a preservative, a stabilizer, a synergist, a carrier, and a solvent;

[0014] Furthermore, the fungicide composition includes, in addition to the active ingredient, an auxiliary ingredient permitted in agriculture, wherein the auxiliary ingredient is selected from one or more of a wetting agent, a dispersant, a thickener, a disintegrant, an emulsifier, a defoaming agent, a preservative, a stabilizer, a synergist, a carrier, and a solvent;

[0015] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0016] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0017] The thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, and carboxymethyl cellulose; and / or

[0018] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0019] The emulsifier is selected from one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenylethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester; and / or

[0020] The defoaming agent is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8~C 10 One or more of fatty alcohol compounds or silicone compounds; and / or

[0021] The preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate; and / or

[0022] The stabilizer is selected from one or more of acid, succinic acid, adipic acid, borax, and epoxidized vegetable oil; and / or

[0023] The synergist is selected from synergist phosphite and synergist ether; and / or

[0024] The carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and white carbon black; and / or

[0025] The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives and deionized water; and / or

[0026] Furthermore, the fungicide composition can be prepared into any formulation permitted in agriculture.

[0027] Furthermore, the dosage form is a solid preparation or a liquid preparation;

[0028] Furthermore, the solid preparation is a direct-use solid preparation, a dispersible solid preparation or a soluble solid preparation;

[0029] Furthermore, the solid preparation for direct use is a powder, granule, pellet, tablet or strip;

[0030] The dispersible solid preparation is a wettable powder, an oil-dispersible powder, an emulsion powder, a water-dispersible granule, an emulsion granule or a water-dispersible tablet;

[0031] The soluble solid preparation is a soluble powder, a soluble tablet or a soluble granule;

[0032] Furthermore, the liquid preparation is a solution preparation, a dispersed liquid preparation, an emulsion preparation, a suspension preparation or a multiphase preparation;

[0033] Furthermore, the solution preparation is a soluble solution, a soluble gel, an oil or a film-spreading oil;

[0034] The dispersed liquid preparation is an emulsifiable concentrate, a latex, a dispersible liquid or an ointment;

[0035] The emulsion preparation is a water emulsion, an oil emulsion, a microemulsion or a lipid preparation;

[0036] The suspension preparation is a suspension concentrate, a microcapsule suspension concentrate, an oil suspension concentrate or a dispersible oil suspension concentrate;

[0037] The multiphase preparation is a suspoemulsion, a microcapsule suspension-suspension concentrate, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0038] Preferably, the solid preparation is a wettable powder and / or a water dispersible granule, and the liquid preparation is a suspension and / or an emulsifiable concentrate.

[0039] The invention also discloses the use of the above-mentioned fungicidal composition and / or its preparation in preventing and controlling plant fungal diseases.

[0040] Furthermore, the plant fungal diseases include one or more of gray mold, powdery mildew, anthracnose, sheath blight, rust, and rice blast;

[0041] Furthermore, the plant fungal disease is gray mold.

[0042] Furthermore, the fungicidal composition is applied to the disease to be controlled or its growth medium at an effective dose.

[0043] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0044] 1) The fungicidal composition of the present invention has a significant synergistic effect on cucumber gray mold;

[0045] 2) The bactericidal composition of the present invention reduces the amount of medicine used and the number of times of use, thereby reducing the cost of prevention and treatment;

[0046] 3) The bactericidal composition of the present invention can effectively control the drug resistance of pathogenic microorganisms and prolong the service life of the agent. DETAILED DESCRIPTION

[0047] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0048] Preparation Example:

[0049] Preparation Example 1: 24% feneptamidoquin·flupyraclostrobin suspension (5:1) (20%+4%)

[0050] Formula composition: feneptamidoquin 20%, flupyraclostrobin 4%, phenethylphenol polyoxyethylene polyoxypropylene ether 3%, glycerol fatty acid ester polyoxyethylene ether phosphate 3%, lignin sulfonate 2%, magnesium aluminum silicate 1%, xanthan gum 0.25%, sodium benzoate 1%, silicone oil 0.5%, ethylene glycol 5%, and deionized water to make up the balance.

[0051] Preparation method: water, dispersant and wetting agent are mixed evenly, defoamer and antifreeze agent are added successively under stirring, mixed evenly by high-speed shearing, feneptamidoquin and fluopyrabamide are added, shearing and mixing are continued, and then the mixture is put into a horizontal sand mill for grinding, and 90% of the particle size of the material is controlled to be below 5 μm, and finally a thickener is added, and the suspension concentrate of the present invention is obtained by shearing evenly.

[0052] Preparation Example 2: 8% feneptamidoquin·flupyraclostrobin emulsifiable concentrate (1:7) (1%+7%)

[0053] Formula composition: feneptamidoquin 1%, fluopicolide 7%, N,N-dimethylformamide 15%, cyclohexanone 20%, castor oil polyoxyethylene ether 12%, calcium dodecylbenzene sulfonate 3%, and xylene to make up the balance.

[0054] Preparation method: The active ingredients feneptamidoquin and fluopyram are dissolved in an organic solvent according to a formula ratio, and then a certain amount of emulsifier is added, and the mixture is stirred and mixed to prepare a homogeneous transparent oily liquid.

[0055] Preparation Example 3: 39% feneptamidoquin·flupyraclostrobin water dispersible granules (12:1) (36%+3%)

[0056] Formula composition: feneptamidoquin 36%, fluopicolide 3%, succinate sulfonate 10%, pull open powder BX 2.5%, lignin sulfonate sodium salt 6%, white sugar 6%, kaolin makes up the balance.

[0057] Preparation method: The active ingredients feneptamidoquin and fluopyrabamide are added to a carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow pulverization, 10-25% of water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and screening.

[0058] Preparation Example 4: 16% feneptamidoquin·boscalid suspension (1:3) (4%+12%)

[0059] Formula composition: 4% feneptamidoquin, 12% boscalid, 2% sorbitan oleate polyoxyethylene ether, 2% fatty alcohol polyoxyethylene ether phosphate, 3% tristyrylphenol polyoxyethylene ether polyoxypropylene ether, 1% sodium polycarboxylate, 0.4% silicone oil, 1% magnesium aluminum silicate, 0.2% sodium sorbate, 0.3% xanthan gum, 5% glycerol, and deionized water to make up the balance.

[0060] Preparation method: water, dispersant and wetting agent are mixed evenly, defoamer and antifreeze agent are added successively under stirring, mixed evenly by high-speed shearing, feneptamidoquin and boscalid are added, shearing and mixing are continued, and then the mixture is put into a horizontal sand mill for grinding, and the particle size of the material is controlled to be 90% below 5 μm, and finally a thickener is added, and the suspension concentrate of the present invention is obtained by shearing evenly.

[0061] Preparation Example 5: 18% feneptamidoquin·boscalid wettable powder (1:8) (2%+16%)

[0062] Formula composition: feneptamidoquin 2%, boscalid 16%, sodium lauryl sulfate 2%, dispersant NNO 5%, sodium lignin sulfonate 5%, white carbon black 3%, and kaolin makes up the balance.

[0063] Preparation method: The active ingredient feneptamidoquin is mixed with boscalid, a dispersant, a wetting agent and a filler, and the mixture is uniformly stirred in a stirring kettle. The mixture is pulverized and mixed uniformly for multiple times in a jet mill to prepare a wettable powder of the composition of the present invention.

[0064] Preparation Example 6: 32% feneptamidoquin·boscalid water dispersible granules (3:1) (24%+8%)

[0065] Formula composition: feneptamidoquin 24%, boscalid 8%, naphthalene sulfonate formaldehyde condensate 10%, sodium lignin sulfonate 5%, sodium lauryl sulfate 4%, diatomaceous earth 5%, and kaolin makes up the balance.

[0066] Preparation method: The active ingredients feneptamidoquin and boscalid are added to a carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow pulverization, 10-25% of water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and sieving.

[0067] Example 1: Indoor biological activity test of gray mold

[0068] Test basis: The test refers to NY / T 1156.2-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth"; NY / T 1156.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 6: Determination of Combined Action of Mixtures".

[0069] Test target: Botrytis cinerea Pers.

[0070] Test agents: feneptamidoquin technical, fluopicolide technical, and boscalid technical.

[0071] Preparation of medicine: Dissolve the above raw medicine in a suitable solvent first, and then dissolve it in a 0.1% Tween-80 aqueous solution, and set the required series of mass concentrations according to the activity of the medicine.

[0072] Drug treatment: Under aseptic operation conditions, add the pre-melted sterile culture medium to the sterile conical flask according to the experimental setting, and quantitatively pipette the drug solution from low concentration to high concentration into the sterile conical flask, and shake it thoroughly. Then pour an equal amount into a culture dish with a diameter of 9 cm to prepare a drug-containing plate of the corresponding concentration.

[0073] Inoculation: Use a sterile puncher to make a bacterial cake from the edge of the activated colony, and use an inoculation loop to inoculate the center of the PDA culture medium of each concentration of the agent and the control, and culture it in a constant temperature incubator for 3 to 4 days. When the control colony is about to fill the entire culture dish, use the cross method to measure the diameter of each colony. Calculate the inhibition rate of each agent on gray mold.

[0074] Data statistics and calculation method: According to the survey results, the mycelial growth inhibition rate of each treatment concentration on the target bacteria was calculated according to the following formula. According to the logarithmic value of each agent concentration and the corresponding inhibition probability value, the statistical analysis system was used to analyze and obtain the toxicity regression equation, EC 50 The activity of the test agent on the biological test material was evaluated by using the value and correlation coefficient.

[0075]

[0076] Where:

[0077] I—colony growth inhibition rate;

[0078] D0—blank control colony growth diameter;

[0079] D t —The growth diameter of the colony after treatment with the drug.

[0080] The co-toxicity coefficient (CTC) of the mixture was calculated according to Sun Yunpei's method to evaluate the type of combined action.

[0081] CTC ≥ 120 indicates synergistic effect, CTC ≤ 80 indicates antagonistic effect, and CTC between 80 and 120 indicates additive effect. The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0082]

[0083] Where:

[0084] ATI——Actual Toxicity Index of Mixture;

[0085] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0086] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0087] TTI=TIA *P A +TI B *P B

[0088] Where:

[0089] TTI – Theoretical Toxicity Index of Mixtures;

[0090] TI A ——A: toxicity index of the agent;

[0091] P A ——The percentage of agent A in the mixture, in percentage (%);

[0092] TI B ——Toxicity index of agent B;

[0093] P B ——The percentage of agent B in the mixture, in percentage (%).

[0094]

[0095] Where:

[0096] CTC – Co-toxicity coefficient;

[0097] ATI——Actual Toxicity Index of Mixture;

[0098] TTI - Theoretical Toxicity Index of Mixture.

[0099] The indoor test results are shown in the table below.

[0100] Table 1 Indoor biological activity test of feneptamidoquin and fluopyram against cucumber gray mold

[0101]

[0102]

[0103] From the results of the indoor combined toxicity test in the above table, it can be seen that fluopicolide has a high toxicity to Botrytis cinerea. 50The total weight of feneptamidoquin and fluopyram was 0.3539 mg / L. When the mass ratio of feneptamidoquin to fluopyram was 1:25-30:1, it had a good control effect on Botrytis cinerea, and the combined effect was synergistic or additive, without antagonism; when the mass ratio of feneptamidoquin to fluopyram was 1:20-30:1, the co-toxicity coefficient was greater than 120, and the combined effect was synergistic; when the mass ratio of feneptamidoquin to fluopyram was 1:15-25:1, the co-toxicity coefficient was greater than 130, and the synergistic effect was obvious; when the mass ratio of feneptamidoquin to fluopyram was 1:10-18:1, the co-toxicity coefficient was greater than 140, and the synergistic effect was significant; when the mass ratio of feneptamidoquin to fluopyram was 5:1, the co-toxicity coefficient was the largest, which was 183.226, indicating that at this mass ratio, the combined activity of feneptamidoquin and fluopyram against Botrytis cinerea was the best.

[0104] Table 2 Indoor biological activity test of feneptamidoquin and boscalid against cucumber gray mold

[0105] Test drug Virulence regression equation Correlation coefficient R <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient Feneptamidoquin(A) y=1.2719x+4.2022 0.9993 4.2390 - Boscalid(B) y=1.2586x+4.4414 0.9955 2.7787 - A:B(1:27) y=1.4283x+4.5096 0.9894 2.2047 127.605 A:B(1:15) y=1.4922x+4.5449 0.9934 2.0183 140.705 A:B(1:8) y=1.4558x+4.6329 0.9912 1.7872 161.666 A:B(1:5) y=1.3987x+4.6733 0.9906 1.7122 172.174 A:B(1:3) y=1.3879x+4.7325 0.9964 1.5586 195.083 A:B(3:1) y=1.3028x+4.6453 0.9971 1.8718 200.168 A:B(5:1) y=1.3290x+4.5337 0.9961 2.2431 173.760 A:B(12:1) y=1.4759x+4.3794 0.9939 2.6332 154.728 A:B(21:1) y=1.3717x+4.2643 0.9974 3.4379 120.425 A:B(24:1) y=1.3285x+4.1909 0.9930 4.0645 102.146 A:B(30:1) y=1.3230x+4.1469 0.9995 4.4143 94.428

[0106] From the results of the indoor combined toxicity test in the table above, it can be seen that boscalid has a high toxicity to Botrytis cinerea. 50 The total weight of feneptamidoquin and boscalid was 2.7787 mg / L. When the mass ratio of feneptamidoquin to boscalid was 1:27-30:1, it had a good control effect on gray mold, and the combined effect showed synergistic or additive, without antagonism; when the mass ratio of feneptamidoquin to boscalid was 1:27-21:1, its co-toxicity coefficient was greater than 120, and the combined effect showed synergistic; when the mass ratio of feneptamidoquin to boscalid was 1:15-12:1, its co-toxicity coefficient was greater than 150, and the synergistic effect was obvious; when the mass ratio of feneptamidoquin to boscalid was 1:8-5:1, its co-toxicity coefficient was greater than 160, and the synergistic effect was prominent; when the mass ratio of feneptamidoquin to boscalid was 3:1, its co-toxicity coefficient was the largest, which was 200.168, indicating that at this mass ratio, the combined activity of feneptamidoquin and boscalid against gray mold was the best.

[0107] Example 2: Field test on efficacy of cucumber gray mold

[0108] Test location: The test is located in the cucumber greenhouse in Sanguanmiao Village, Pulandian City, Dalian City, Liaoning Province. The soil fertility of the test site is medium, and the cultivation management of each test plot is relatively uniform.

[0109] Experimental crops: cucumber (Xintai Mici).

[0110] Test object: Cucumber gray mold (Botrytis cinerea). The occurrence of gray mold was common and uniform in all test plots.

[0111] Experimental treatment: The experiment set up 8 treatments, each treatment was repeated 4 times, and the plot area was 20m 2 .

[0112] Application method: The test used a backpack sprayer for spraying, and the spraying machine used a Singapore Linong sprayer with a fan-shaped nozzle and a spray pressure of 1 atmosphere. The first application of the drug was at the early stage of cucumber gray mold, and the second application was made after an interval of 7 days, for a total of two applications.

[0113] Test method: Fixed-point survey was adopted in each plot, 30 plants were surveyed in each plot, all fruits of each plant were surveyed, the disease situation was investigated 7 days after the first application of pesticide and 10 days after the second application of pesticide, and the diseased fruits and disease level were recorded.

[0114] Cucumber fruit damage classification method (based on fruit):

[0115] Level 0: no lesions;

[0116] Level 1: Disease on residual flowers;

[0117] Grade 3: Disease occurs in the navel;

[0118] Level 5: The length of the lesion accounts for less than 10% of the fruit;

[0119] Level 7: The length of the lesion accounts for 11% to 25% of the fruit;

[0120] Level 9: The length of the lesion accounts for more than 26% of the fruit.

[0121]

[0122] The field efficacy test is shown in the following table:

[0123] Table 3 Results of field efficacy test on cucumber gray mold

[0124]

[0125]

[0126] From the test results in the above table, it can be seen that the reasonable combination of feneptamidoquin with fluopyram or boscalid for the prevention and control of cucumber gray mold has very excellent control effect, which is significantly better than the control effect of 300g / L fluopyram suspension, 50% boscalid water dispersible granules and 25% feneptamidoquin suspension. The control effect of the compound preparation after two applications is 87.40%, 83.00%, 90.27% and 87.27%, respectively. Each treatment test agent has no adverse effect on the growth and results of cucumber plants at the applied dosage.

[0127] It can be seen from indoor toxicity tests and field efficacy tests that the fungicide composition of the present invention exhibits good control effect on cucumber gray mold, is safe for crops, delays the development of drug resistance in pathogenic bacteria, reduces the dosage of the agent used and reduces the agent residue in agricultural products.

[0128] Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A bactericidal composition containing feneptamidoquin, characterized in that: The fungicide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is feneptamidoquin, the active ingredient B is any one of fluopyraclostrobin or boscalid, and the mass ratio of the active ingredient A to the active ingredient B is 1:27 to 30:

1.

2. The bactericidal composition according to claim 1, characterized in that The active ingredient B is fluopicolide, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1; The active ingredient B is boscalid, and the mass ratio of the active ingredient A to the active ingredient B is 1:27 to 21:

1.

3. The bactericidal composition according to claim 1, characterized in that The active ingredient B is fluopicolide, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 25:1; The active ingredient B is boscalid, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 12:

1.

4. The bactericidal composition according to claim 1, characterized in that The total weight of the bactericidal composition is 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 0.1% to 80%, preferably 1% to 70% of the total weight of the bactericidal composition.

5. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicide composition also includes agriculturally permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, thickeners, disintegrants, emulsifiers, defoamers, preservatives, stabilizers, synergists, carriers, and solvents.

6. The bactericidal composition according to claim 1, characterized in that The bactericidal composition can be prepared into any formulation form permitted in agriculture.

7. The bactericidal composition according to claim 6, characterized in that The dosage form is a solid preparation or a liquid preparation; Preferably, the solid preparation is a wettable powder or a water dispersible granule, and the liquid preparation is a suspension or an emulsifiable concentrate.

8. Use of the fungicidal composition and / or its preparation according to any one of claims 1 to 7 in preventing and controlling plant fungal diseases.

9. The use according to claim 8, characterized in that: The plant fungal diseases include one or more of gray mold, powdery mildew, anthracnose, sheath blight, rust, and rice blast; Preferably, the plant fungal disease is gray mold.

10. The use according to claim 8, characterized in that: The fungicide composition is applied to the disease to be controlled or its growth medium at an effective dose.

Citation Information

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