Traditional Chinese medicine composition for treating joint swelling and pain and preparation method thereof

Abstract

A traditional Chinese medicine composition for treating joint swelling and pain and a preparation method thereof belong to the technical field of joint swelling and pain treatment, and the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials by weight: 15g of herba siegesbeckiae, 15g of climbing entada, 10g of berba aristolochiae mollissimae, 10g of spiderflower seed, 15g of dog tibia, 10g of cucumber seed, 10g of bamboo leaf, 15g of radix sophorae flavescentis, 15g of earthworm, 15g of turmeric, 6g of prepared nux vomica, 10g of sappan wood, 15g of menthol, 15g of garden balsam seed, 10g of dragon's blood and 10g of safflower. All the medicines are ground into powder, then a certain proportion of water is added, the mixture is blended into paste, and the traditional Chinese medicine external application plaster is prepared. All the components are combined to achieve the effects of dispelling wind and dampness, promoting blood circulation to remove blood stasis and activating meridians to stop pain.

Description

Technical Field

[0001] The invention belongs to the technical field of treating joint swelling and pain, and specifically relates to a traditional Chinese medicine composition for treating joint swelling and pain and a preparation method thereof. Background Art

[0002] Painful swelling of joint refers to swelling, redness, fever and limited movement around joints. It is the clinical manifestation of many diseases. It mainly manifests as effusion and pain in the knee, ankle, wrist, elbow and other joints. Different treatment methods are used according to different diseases, and drugs are used to relieve pain.

[0003] Chinese patent application CN111297980 A discloses a Chinese medicine composition, preparation and preparation method for treating joint swelling and pain. The composition includes the following components by weight: 10-20 parts of castor beans, 25-35 parts of strychnine seeds, 10-20 parts of clematis root, 15-25 parts of aconite root, 55-80 parts of zushima, 20-40 parts of processed aconite, 20-45 parts of radix scutellariae, 5-30 parts of chuanxiong, 8-30 parts of angelica root and 1-20 parts of frankincense. The composition claims to be able to effectively relieve or treat joint pain caused by wind, cold and dampness, especially joint swelling and pain, with significant efficacy.

[0004] Chinese patent application CN113144048 A discloses a Chinese medicine external patch for treating gouty joint swelling and pain, including backing material, medicine, matrix and anti-adhesion layer, wherein medicine includes 30-35 parts of Cortex Phellodendri, 20-25 parts of Radix Gentianae Macrophyllae, 30-35 parts of Radix Sarcandrae, 20-25 parts of Rhizoma Atractylodis Macrocephalae, 30-35 parts of Radix Clematidis, 20-25 parts of Radix Et Rhizoma Rhei, 20-25 parts of Folium Isatidis, 15-20 parts of Radix Trichosanthis, and 5-7.5 parts of Borneolum Syntheticum. Raw material ultrafine grinding is into fine powder, mixed with matrix, then coated on backing material with coating machine, surface covering anti-adhesion layer, namely obtains the Chinese medicine external patch for treating gouty joint swelling and pain. The patch is directly attached to the affected part, claims to effectively prevent or alleviate the joint swelling and pain caused by gout and improve the nodules formed by gout, easy to use, good patient compliance, no stimulation, suitable for multiple medication. Summary of the invention

[0005] The invention aims to provide a traditional Chinese medicine composition for treating joint swelling and pain. The traditional Chinese medicine composition is composed of siegesbeckia, kudzuvine, xungusi, cauliflower seeds, dog tibia, cucumber seeds, bamboo leaves, sophora flavescens, earthworms, turmeric, processed horse chestnut seeds, sappan wood, menthol, fujianzi, dragon's blood and safflower. The traditional Chinese medicine composition is applied externally to swollen joints to achieve the effects of clearing away heat, detoxicating, relieving swelling and relieving pain.

[0006] The technical solution adopted by the present invention is:

[0007] A traditional Chinese medicine composition for treating joint swelling and pain is prepared from the following traditional Chinese medicine raw materials by weight: 15g of Siegesbeckia sibiricum, 15g of Longan Longan, 10g of Xungusifolia, 10g of white flower vegetable seeds, 15g of dog tibia, 10g of cucumber seeds, 10g of bamboo leaves, 15g of Sophora flavescens, 15g of earthworms, 15g of turmeric, 6g of processed horse chestnut seeds, 10g of Sappan wood, 15g of menthol, 15g of Aconitum carmichaelii, 10g of Sanguisorba officinalis, and 10g of safflower.

[0008] The preparation method of the Chinese medicine composition specifically comprises the following steps: grinding each medicine into powder, then adding a certain proportion of water to mix into a paste, and preparing the Chinese medicine external application plaster.

[0009] Drugs such as Siegesbeckia, Longganglong, Xungufeng, Baihuacaizi, and bamboo leaves have anti-inflammatory and analgesic effects. The terpenoid compounds contained in Siegesbeckia have anti-inflammatory, analgesic, and immune-regulating effects. The triterpenoid compounds and flavonoid compounds contained in Longganglong have anti-inflammatory, analgesic, antioxidant, and anti-tumor effects. Xungufeng may promote the elimination of inflammation by increasing the level of anti-inflammatory factor IL-2 in the serum of rats with adjuvant arthritis, reducing the level of inflammatory factor TNF-α, and improving the body's anti-inflammatory effect. Baihuacaizi contains triterpenoids, flavonoids, and glucose isothiocyanate compounds, and the methanol extract of Baihuacai leaves has obvious anti-adjuvant-induced arthritis effects. The terpenoids and steroid compounds contained in bamboo leaves have anti-inflammatory effects.

[0010] Dog tibia, cucumber seeds, strychnos nux vomica, sappan wood, dragon's blood, safflower and other drugs have the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain. Dog tibia and cucumber seeds can heal tendons and fractures. Dragon's blood can increase the number of Treg lymphocytes in the spleen of CIA mice and reduce the number of Th17, correct the imbalance of Treg / Th17, inhibit the secretion of inflammatory factor IL-17, and thus alleviate the progress of arthritis. Safflower extract can reduce the secretion of prostaglandin 2 and exert anti-inflammatory and analgesic effects. Strychnine in strychnos nux vomica and hematoxylin in sappan wood have anti-inflammatory and immune-regulating effects in modern pharmacological research. Strychnos nux vomica has anti-inflammatory, anti-tumor, and immune-regulating effects.

[0011] Pheretima and turmeric have the effect of promoting blood circulation and unblocking collaterals. Pheretima can effectively reduce the VAS score, knee joint function index score, inflammatory factor index, and bone metabolism index of patients with osteoarthritis. Curcumin can alleviate LPS-induced inflammatory response by inhibiting the activation of TLR4 / MyD88 / NF-κB signaling axis.

[0012] Drugs such as Sophora flavescens and menthol have anti-allergic effects. Matrine is an alkaloid extracted from Sophora flavescens, which has multiple pharmacological effects such as anti-inflammatory, antioxidant, antiviral, anti-allergic, anti-tumor, anti-fibrosis, anti-arrhythmic, and immunomodulatory. It is used in the treatment of hepatitis, tumors, and cardiovascular diseases. Matrine is widely used in the treatment of skin diseases such as eczema, atopic dermatitis, psoriasis, and psoriasis. Matrine can regulate immune responses, reduce inflammatory damage, enhance cell autophagy, and promote cell apoptosis. Menthol is a mint extract that has anti-allergic effects and can reduce the excitability of the nervous system by interacting with receptors on sensory nerve cells.

[0013] The Chinese medicine composition for treating joint swelling and pain proposed by the present invention has the following treatment principles: Herba Siegesbeckiae is cold in nature, bitter and pungent in taste, and enters the liver and kidney meridians, and has the effects of dispelling rheumatism, benefiting joints, reducing swelling and detoxifying. It is clinically used to treat rheumatic arthralgia. Herba Glechomae is warm in nature, pungent in taste, and bitter in taste, and enters the liver and stomach meridians, and has the effects of dispelling wind and dampness, promoting blood circulation and unblocking collaterals, and is clinically used to treat joint pain caused by arthralgia. Herba Xungufeng is flat in nature, pungent in taste, and bitter in taste, and enters the liver and stomach meridians. Herba Glechomae is warm in nature, pungent in taste, and bitter in taste, and enters the heart and spleen meridians, and can treat pain in the waist and legs. Herba Glechomae is warm in nature, pungent in taste, and salty in taste, and has the effects of dispelling wind and dampness, relaxing tendons and unblocking collaterals, promoting blood circulation and relieving pain, and strengthening tendons and bones. Herba Cucumidis Cucumidis has the effect of supplementing calcium and strengthening bones. Herba Aconitum, Semen Strychnos, Sappan Wood, Dracaena Dracaenae, and Safflower have the effects of promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, and clinically have a certain effect on meridian obstruction and joint pain caused by qi stagnation and blood stasis. Pheretima and turmeric have the effect of promoting blood circulation and unblocking meridians, and clinically have certain effects on arthritis caused by wind, cold, dampness and blood stasis. Sophora flavescens has the effects of dispelling wind, clearing heat and drying dampness, and menthol has the effects of clearing heat, dispelling wind and relieving itching. The combination of the two can treat skin itching and joint pain caused by dampness and heat. The whole formula can be used together to dispel wind and dampness, promote blood circulation and remove blood stasis, unblock meridians and relieve pain. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 Comparison of toe swelling before and after treatment.

[0015] Figure 2 To compare the arthritis index before and after treatment.

[0016] Figure 3 Comparison of ESR before and after treatment.

[0017] Figure 4 Comparison of TNF-α before and after treatment.

[0018] Figure 5 Comparison of IL-6 before and after treatment. DETAILED DESCRIPTION

[0019] The following is a further detailed description of a Chinese medicine composition for treating joint swelling and pain and a preparation method of the present invention in conjunction with the accompanying drawings and examples.

[0020] In this experiment, a collagen-induced arthritis rat model was prepared by establishing a type II collagen emulsion immune induction method, and the effects of external application of traditional Chinese medicine on the level of arthritis, degree of toe swelling, erythrocyte sedimentation rate, and levels of inflammatory factors in the model rats were studied.

[0021] 1. Materials and Methods

[0022] 1.1 Animals

[0023] SD rats, male, clean grade, weighing 180-200 g, were purchased from Shanghai Xipu-Bikai Experimental Animal Co., Ltd.

[0024] 1.2 Main reagents and instruments

[0025] Bovine type II collagen (Chondrex); chloral hydrate; fully automatic biochemical analyzer (Anhui Provincial Hospital of Traditional Chinese Medicine); sample homogenizer (Dragonlab, USA); desktop high-speed refrigerated centrifuge (Eppendorf, Germany).

[0026] 1.3 Plaster prescription

[0027] Plaster prescription: Herba Siegesbeckiae 15g, Herba Glehniae 15g, Herba Xungifongae 10g, Herba Glehniae 10g, Herba Glehniae 10g, Herba Glehniae 15g, Herba Cucumidis 10g, Herba Bambusae 10g, Herba Sophorae Flavescentis 15g, Herba Pheretimae 15g, Herba Curcumae 15g, Herba Erectae 6g, Herba Sappani 10g, Herba Menthol 15g, Herba Aconitum 15g, Herba Draconis 10g, Herba Safflower 10g. This is the prescription agreed by the Department of Rheumatology and Immunology of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, and the Chinese medicinal materials used are provided by the Chinese Medicine Pharmacy of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine. The above drugs are ground into powder and mixed into a paste by adding a certain proportion of water.

[0028] 1.4 Methods

[0029] 1.4.1 Modeling

[0030] SD rats were given bovine type II collagen emulsion twice to establish a collagen-induced arthritis rat model. After the rats were anesthetized with chloral hydrate, bovine type II collagen emulsion was injected intradermally at the base of the tail and back (0.2 mL / rat) for primary immunization. Two weeks after the primary immunization, bovine type II collagen emulsion was injected intradermally at the base of the tail and back (dose 0.3 mL / rat) for booster immunization. The rats were raised for 2 weeks in an environment with a constant temperature of 37°C, a humidity of 90%, a blower machine, and a light duration of 12 hours to form a rat model of joint swelling.

[0031] 1.4.2 Administration

[0032] The control group was given Xinguang tablets (Xiamen Chinese Medicine Factory Co., Ltd., National Medicine Standard Z35020063), 40 mg / time, ground into powder, mixed with water into a paste, and applied to the swollen joints of mice. The experimental group was given a Chinese medicine plaster on the basis of the control group, applied once a day for 6 hours, and the experimental period was 4 weeks. The various indicators of the rats were observed every day for four weeks.

[0033] 1.5 Observation indicators and detection methods

[0034] 1.5.1 Degree of swelling of toes

[0035] The degree of swelling was determined by the volume of the toes after modeling, and the changes in the toe volume of each group were recorded before and after 4 weeks of plaster application. The displacement method was used to measure the rats in each group. The rats' hind limbs were immersed in a graduated glass container filled with water, just covering the ankle joints of the rats. The measurement was repeated 3 times, and the displacement was recorded each time and the average value was taken.

[0036] 1.5.2 Arthritis Index

[0037] The arthritis index was recorded before and after 4 weeks of plaster application, and scored in the form of points, with normal without arthritis as 0 points; redness or slight swelling as 1 point; moderate swelling as 2 points; severe swelling as 3 points; severe swelling and inability to bear weight as 4 points. The total score of each rat's limbs was calculated.

[0038] 1.5.3 Blood IL-6, TNF-α, and erythrocyte sedimentation rate (ESR).

[0039] Immunofluorescence was used to detect the expression of ESR, IL-6, and TNF-α in rats. Blood was collected from the heart and the supernatant was centrifuged. The concentrations of ESR, IL-6, and TNF-α in rats were measured using an automatic biochemical analyzer. All instrument parameters were kept fixed during the measurement, and all processes were carried out strictly in accordance with the experimental steps.

[0040] 2. Results

[0041] 2.1 Degree of toe swelling

[0042] The changes in the toe volume of rats in each group were measured before and after 4 weeks of plaster application. Compared with the control group, the toe volume of rats in the study group decreased significantly (p<0.05), and the difference was statistically significant (see Table 1, Figure 1 .

[0043] Table 1 Comparison of toe swelling before and after treatment

[0044]

[0045] 2.2 Arthritis Index

[0046] The arthritis of the study group was significantly reduced before and after 4 weeks of plaster application (p<0.05), and the difference was statistically significant, as shown in Table 2. Figure 2 .

[0047] Table 2 Comparison of arthritis index before and after treatment

[0048]

[0049] 2.3 Serum ESR, TNF-α, IL-6

[0050] The expression of serum IL-1, IL-6 and TNF-α in each group was measured before and 4 weeks after external application of the plaster. The expression of IL-1, TNF-α and IL-6 in the research group decreased significantly (p<0.05), and the difference was statistically significant (see Table 3, Figure 3 , Figure 4 , Figure 5 .

[0051] Table 3 ESR, IL-6, TNF-α before and after treatment contrast

[0052]

[0053] The present invention has found through experiments that external application of Chinese medicine plaster can help significantly improve the arthritis index and toe swelling symptoms of collagen-induced arthritis rats, and can reduce the levels of ESR, TNF-α, and IL-6, indicating that the plaster compound can improve the inflammation of collagen-induced arthritis rats by reducing inflammatory factors, thereby reducing the degree of joint swelling.

[0054] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. A Chinese medicine composition for treating joint swelling and pain, characterized in that: It is prepared from the following Chinese medicinal raw materials by weight: 15g of Siegesbeckia scabra, 15g of Longkonglong, 10g of Xungusifolia, 10g of white flower vegetable seeds, 15g of dog tibia, 10g of cucumber seeds, 10g of bamboo leaves, 15g of Sophora flavescens, 15g of earthworms, 15g of turmeric, 6g of processed Semen Armeniacae Amaranthus, 10g of Sappan wood, 15g of menthol, 15g of Aconitum, 10g of Sanguisorba officinalis, and 10g of safflower.

2. A method for preparing the Chinese medicine composition according to claim 1, characterized in that: Grind each medicine into powder, then add a certain proportion of water to mix into a paste to make a Chinese medicine external application plaster.

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