Cupriavidus rhizosphere Basella sp. 10 and application thereof in phosphorus solubilization and growth promotion

Abstract

The invention relates to the technical field of microorganisms, in particular to rhizosphere Cupriavidus sp. 10 and application thereof in the aspects of phosphorus solubilization and growth promotion. The preservation number of the rhizosphere Cupriavidus sp. 10 provided by the invention is GDMCC No.65501. The rhizosphere Cupriavidus sp. 10 can improve the content of chlorophyll a, chlorophyll b, carotenoid and total chlorophyll in the leaves of the seedlings of amur corktree, and increase the plant height, ground diameter, total fresh weight, fresh root weight, fresh stem weight, fresh leaf weight, total root length and number of thick roots of the seedlings of amur corktree. Meanwhile, accumulation of soluble sugar and available phosphorus in roots and leaves is promoted.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to a rhizosphere Cuproplastis baselense-10 and applications thereof in phosphorus dissolution and growth promotion. Background Art

[0002] Phellodendron chinense Schneid is an important perennial woody medicinal material, widely distributed in Hunan, Hubei and Sichuan in southern China. This plant can usually grow to 10-12m high, with young branches purple-brown and dark brown-gray trunk epidermis with white lenticels on the surface and no hair. The leaves of Phellodendron chinense Schneid are odd-pinnate compound leaves, which are opposite, and the rachis, petiole and back of the leaves are covered with fine hairs. As a dioecious plant, Phellodendron chinense Schneid blooms light yellow unisexual flowers between May and June. The stem bark of Phellodendron chinense contains active ingredients such as alkaloids, flavonoids, and sterols, among which alkaloids have the highest content. The alkaloids in the stem bark of Phellodendron chinense mainly include berberine, phellodendronine, bamipine and other components, among which berberine has the highest content. It has significant anti-tumor, anti-inflammatory and antibacterial, hypoglycemic and lipid-lowering effects, and is widely used in the treatment of cancer, enteritis, diabetes, arthritis and other diseases. The growth and medicinal ingredient content of Phellodendron amurense are affected by rhizosphere growth-promoting bacteria, nutrients, site conditions and other factors, which in turn affect its yield and economic value.

[0003] Phosphorus (P) is one of the key nutrients required for plant growth. It plays an important role in photosynthetic phosphorylation, nucleic acid synthesis, protein phosphorylation modification, and other processes, thereby regulating plant growth, development, stress response, and secondary metabolism. Plants mainly absorb phosphorus from the soil, and available phosphorus is easily absorbed by Fe. 2+ , Ca 2+ 、Al 3+ The combination of metal ions such as phosphorus and phosphorus forms insoluble compounds that cannot meet the needs of plant growth. The soil in Hunan Province is mainly red soil, which has a high total phosphorus content and a low available phosphorus content (≤0.01%), which limits the growth of plants and the synthesis of important secondary metabolites. Therefore, the content of available phosphorus in the soil can be effectively supplemented by applying phosphorus fertilizers, but it is easy to cause environmental problems such as eutrophication. On the other hand, the addition of rhizosphere phosphate-dissolving bacteria can effectively alleviate the limiting effect of soil phosphorus. Among them, phosphate-dissolving bacteria such as Pseudomonas moraviensis, Bacillus safensis, and Falsibacillus pallidus have significant phosphate-dissolving ability and growth-promoting functions.

[0004] However, different types of plants enrich different types of rhizosphere growth-promoting bacteria, and there are no reports on the isolation, identification and growth-promoting functions of rhizosphere growth-promoting bacteria in the Chinese medicinal material Phellodendron chinense. Therefore, it is urgent to study a bacterium that can improve the phosphorus solubility capacity of the rhizosphere soil of Phellodendron chinense and promote the growth of seedlings. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a rhizosphere Basel Cupribotium-10 and its application in phosphorus solubilization and growth promotion. The rhizosphere Basel Cupribotium-10 can increase the contents of chlorophyll a, chlorophyll b, carotenoids and total chlorophyll in the leaves of Phellodendron amurense seedlings, increase the plant height, ground diameter, total fresh weight, root fresh weight, stem fresh weight, leaf fresh weight, total root length and number of thick roots of Phellodendron amurense seedlings, and promote the accumulation of soluble sugars and available phosphorus in roots and leaves.

[0006] To this end, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides a rhizospheric Cupriavidus baselense-10 in an optional embodiment, and the deposit number of the rhizospheric Cupriavidus baselense-10 is GDMCC No:65501.

[0008] In the present invention, the deposit number of rhizospheric Cupriavidus baselensis-10 is GDMCC No: 65501, the deposit date is November 19, 2024, the deposit center is Guangdong Microbial Culture Collection Center, the deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Institute of Microbiology, Guangdong Academy of Sciences, the deposited classification name is Cupriavidusbasilensis CB-10, and the deposited survival state is alive.

[0009] Preferably, the nucleotide sequence of the rhizospheric Cupriavidae baselense-10 is shown as SEQ ID NO.1.

[0010] In a second aspect, the present invention provides, in an optional embodiment, a rhizosphere Cupribotium baselense-10 bacterial agent, which is prepared using the above-mentioned rhizosphere Cupribotium baselense-10.

[0011] In a third aspect, the present invention provides a method for preparing the above-mentioned rhizosphere Cupricobacterium Basel-10 bacterial agent in an optional embodiment, comprising the following steps:

[0012] The rhizosphere copper-grasping bacteria-10 was inoculated into the culture medium for cultivation. 600 When it is 0.7, the rhizospheric Basel Cupric Bacteria-10 agent is obtained.

[0013] Preferably, the culture temperature is 25-30°C, the culture time is 8-12h, and the rotation speed is 150-250rpm; and / or the culture medium is R2A liquid culture medium.

[0014] The third invention, in an optional embodiment, the present invention provides a use of the above-mentioned rhizosphere Cuprifolia spp.-10 or the above-mentioned rhizosphere Cuprifolia spp.-10 bacterial agent in promoting the growth of Phellodendron amurense seedlings.

[0015] Preferably, the indicators for promoting the growth of Phellodendron amurense seedlings include plant height, ground diameter, total fresh weight, leaf fresh weight, stem fresh weight, root fresh weight, total root length and number of thick roots of the Phellodendron amurense seedlings; the method for promoting the growth of Phellodendron amurense seedlings with the rhizosphere Basel Cupric Bacterium-10 agent is: 20-30 mL of the rhizosphere Basel Cupric Bacterium-10 agent is inoculated into each Phellodendron amurense seedling grown to the four-leaf stage by root irrigation.

[0016] In a fourth aspect, the present invention provides, in an optional embodiment, a use of the above-mentioned rhizosphere Cuprifolia spp.-10 or the above-mentioned rhizosphere Cuprifolia spp.-10 agent in improving the pigment content of leaves of Phellodendron amurense seedlings.

[0017] Preferably, the pigments include chlorophyll a, chlorophyll b, carotenoids and total chlorophyll.

[0018] In a fifth aspect, the present invention provides, in an optional embodiment, a use of the above-mentioned rhizospheric Cupribotium-10 or the above-mentioned rhizospheric Cupribotium-10 agent in regulating the content of soluble substances in the leaves and roots of Phellodendron amurense seedlings.

[0019] Preferably, the soluble substances include soluble sugars and fructose. In the present invention, rhizosphere Cupribotium basilicum-10 or rhizosphere Cupribotium basilicum-10 agent can increase the content of soluble sugars in leaves and roots of Phellodendron amurense seedlings, and can also increase the content of fructose in roots of Phellodendron amurense seedlings.

[0020] In a sixth aspect, the present invention provides, in an optional embodiment, a use of the above-mentioned rhizosphere Cupribotium-10 or the above-mentioned rhizosphere Cupribotium-10 agent in increasing the effective phosphorus content in the leaves of Phellodendron amurense seedlings.

[0021] The nucleotide sequence shown in SEQ ID NO.1 is as follows:

[0022]

[0023] Compared with the prior art, the present invention has one of the following beneficial effects:

[0024] 1. The rhizosphere Cupricobacterium baselense-10 provided by the present invention can increase the contents of chlorophyll a, chlorophyll b, carotenoids and total chlorophyll in the leaves of Phellodendron amurense seedlings, increase the plant height, ground diameter, total fresh weight, root fresh weight, stem fresh weight, leaf fresh weight, total root length and number of thick roots of Phellodendron amurense seedlings, and promote the accumulation of soluble sugars and available phosphorus in roots and leaves. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a photo of the rhizosphere Cupricobacterium baselense-10 isolated and purified in Example 1 of the present invention, wherein Figure 1 A is the front view of the rhizosphere Cupricobacterium baselense-10. Figure 1 B is the reverse view of the rhizosphere Cupriobacterium baselense-10.

[0026] Figure 2 This is a schematic diagram of the results of analyzing the homology of rhizospheric Cupria subscapholuns-10 based on the neighbor-joining method in Example 1 of the present invention;

[0027] Figure 3 This is a schematic diagram of the results of analyzing the homology of rhizospheric Cupria subscapholuns-10 based on the minimum evolution method in Example 1 of the present invention;

[0028] Figure 4 This is a schematic diagram of the results of analyzing the homology of rhizospheric Cupria subscapholuns-10 based on the maximum parsimony method in Example 1 of the present invention;

[0029] Figure 5 This is a schematic diagram of the results of the phosphorus solubilization effect of the rhizosphere Cupricobacterium baselense-10 in Example 2 of the present invention;

[0030] Figure 6 This is a schematic diagram of the effect of rhizospheric Cupricobacterium baselense-10 on promoting the growth of Phellodendron amurense seedlings in Example 4 of the present invention;

[0031] Figure 7 This is a schematic diagram of the effect of rhizospheric Cupricobacterium baselense-10 in improving the pigment content of leaves of Phellodendron amurense seedlings in Example 4 of the present invention;

[0032] Figure 8 This is a schematic diagram of the effect of rhizospheric Cupricobacterium baselense-10 in regulating the content of soluble substances in leaves and roots of Phellodendron amurense seedlings in Example 4 of the present invention;

[0033] Fig. 9 This is a schematic diagram of the effect of rhizospheric copper bacteria basel-10 in improving the available phosphorus content in leaves of Phellodendron amurense seedlings in Example 4 of the present invention;

[0034] Fig.10 The phenotype diagram of Phellodendron amurense seedlings cultured in CK group and CB-10 group in Example 4 of the present invention. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0036] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0037] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0038] The preparation method of R2A solid culture medium is as follows: accurately weigh 18.12g of R2A agar culture medium powder (purchased from Haibo Biotechnology Co., Ltd.) and dissolve it in distilled water, and dilute to 1L. Then move the prepared culture medium to the autoclave and sterilize it at 121°C for 15 minutes. After the culture medium is sterilized, take it out and disinfect it with 75% ethanol solution and place it on the clean bench to cool. When the temperature drops to 40-50°C, add sterile amphotericin B (addition amount is 0.8mg / L), mix well and dispense it into culture dishes for later use.

[0039] The preparation method of R2A liquid culture medium is as follows: accurately weigh 3.2g of R2A liquid culture medium powder (purchased from Haibo Biotechnology Co., Ltd.), dissolve it in pure water, and dilute it to 1L with pure water. Transfer the prepared culture medium to the sterilizer and sterilize it at 121℃ for 15min. After sterilization, cool the culture medium to 40-50℃ on the clean bench, then add sterile amphotericin B (addition amount is 0.8mg / L), shake it thoroughly and dispense it into 50mL sterilized conical bottles for use.

[0040] The main components of inorganic phosphorus bacterial culture medium are: glucose 10g / L, (NH4)2SO40.5 g / L, NaCl 0.3g / L, KCl 0.3g / L, MgSO4·7H2O 0.3g / L, FeSO4·7H2O 0.03g / L, MnSO4·4H2O 0.03g / L, Ca3(PO4)25g / L, distilled water 1L, pH 7.0.

[0041] Example 1

[0042] This embodiment provides a method for preparing rhizospheric copper-grabbing bacteria-10, comprising the following steps:

[0043] S1: Weigh 0.2g of fresh soil sample (collected from the rhizosphere soil 1mm from the root of one-year-old Phellodendron amurense in the Xiyuan experimental field of Central South University of Forestry and Technology), transfer it to a conical bottle containing 50mL of sterilized water, cover the bottle mouth with a sealing film, and fix it with a rubber band. Then place the conical bottle containing the rhizosphere soil sample in an ultrasonic cleaner and fully shake it at room temperature for 3min (the shaking speed is 100rpm / min) to obtain a soil bacterial suspension.

[0044] S2: Take 1mL of soil bacterial suspension, centrifuge at 6000rpm for 3min, and collect the supernatant. Take 50μL of supernatant, evenly spread it on the surface of R2A solid culture medium, seal the culture dish with sealing film, and place it in a constant temperature incubator (temperature is 28℃) for 24h. Then pick a single colony on the R2A solid culture medium, purify it repeatedly and observe the growth morphology of the single colony to obtain a single rhizosphere bacterium. A total of 20 independent rhizosphere bacterial strains were isolated.

[0045] S3: The isolated independent rhizospheric bacterial colonies were inoculated into R2A liquid medium. After culturing for 11 h, 500 μL of bacterial solution was taken and mixed with 500 μL of glycerol solution. After labeling, the mixture was quickly frozen in liquid nitrogen and then transferred to a -80°C refrigerator for storage. The results are shown in Figure 1 .

[0046] S4: The isolated rhizospheric bacterial genomic DNA was extracted using a bacterial genomic DNA extraction kit (Omega Bio-Tek Anolun (Beijing) Biotechnology Co., Ltd.), and the 16S rDNA sequence was PCR amplified using primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-CTACGGTACCTT GTTACGA-3'). The amplified fragments were then sequenced on the Illimina NovaSeq 6000 platform (Shenzhen Micro-Cam Technology Group Co., Ltd.). The 16S rDNA sequence of the sequenced strain was compared with the NCBI nucleic acid database by BLAST analysis, and the phylogenetic tree was constructed and sequence homology was analyzed using MEGA5.1 software based on the neighbor-joining method, minimum evolution method, and maximum parsimony method. The results are shown in Figure 2-Figure 4 According to the 16S sequence analysis results, Bacteria 10 was named Cupriavidus basilensis-10 (CB-10), i.e., the rhizosphere Cupriavidus basilensis-10 of this embodiment.

[0047] Example 2

[0048] This embodiment provides a test on the phosphorus solubilization effect of rhizosphere Cupricobacterium baselense-10 prepared based on Example 1, and the specific steps are as follows:

[0049] (1) Preparation of potassium antimony tartrate solution: Weigh 0.5 g of potassium antimony tartrate [K(SbO)C4H4O6] and dissolve it in 100 mL of pure water to prepare a potassium antimony tartrate solution with a concentration of 0.5%.

[0050] (2) Prepare molybdenum-antimony mixed solution: Take 10g of ammonium molybdate [(NH4)6Mo7O 24 ·4H2O], dissolve in 450mL pure water, slowly add 153mL concentrated sulfuric acid, stirring while adding. Then add 100mL of 0.5% potassium antimony tartrate solution, and finally add water to make up to 1L, shake well, and store in a brown bottle.

[0051] (3) Preparation of molybdenum-antimony anti-colorimetric agent: Before use (i.e., prepare on the same day), weigh 1.5 g of L-ascorbic acid, dissolve it in 100 mL of molybdenum-antimony mixed solution, mix well, and the effective period is 24 hours.

[0052] (4) 100 μL of the cultured rhizosphere copper bacteria-10 was added to 50 mL of inorganic phosphorus liquid culture medium (culture group), and the culture medium and 50 mL of inorganic phosphorus liquid culture medium (control group) were shaken and cultured at 37 °C and 160 rpm / min for 3 days to obtain fermentation liquid. Then 10 mL of the fermentation liquid was aspirated and transferred to a centrifuge tube, centrifuged at 5000 rpm / min for 10 min and the supernatant was collected. Then 5 mL of the supernatant was aspirated into a 50 mL volumetric flask and diluted to 20 mL with sterile distilled water. Three drops of 2,6-dinitrophenol indicator were slowly added, followed by a 10% sodium hydroxide solution or a 10% dilute sulfuric acid solution to adjust the pH of the solution until the solution was just slightly yellow. Finally, 5 mL of molybdenum antimony anti-colorimetric agent was added, shaken and placed at room temperature for 30 min, and then the absorbance was measured at 680 nm to calculate the content of available phosphorus. The result of the culture group was 0.30 mg·L -1 The result of the control group was 0.07 mg·L -1 , the results are shown in Figure 5 .

[0053] pass Figure 5 It can be seen that the available phosphorus content in the rhizospheric Cupribotium-10 added group was 4.31 times that of the control group (P<0.05), and the rhizospheric Cupribotium-10 had a strong phosphorus solubilizing ability.

[0054] Example 3

[0055] This embodiment provides a method for preparing a bacterial agent of rhizosphere Cupricobacterium baselense-10 prepared in Example 1, comprising the following steps:

[0056] (1) Activate the rhizosphere Cupribothrium baselense-10 stored at -80°C on R2A medium;

[0057] (2) A single colony of Basel's copper-grabbing bacteria-10 was selected from the rhizosphere and inoculated into a conical flask containing 20 mL of R2A liquid culture medium. The culture was cultured at 28 °C and 200 rpm / min for 10 h. 600 When the value is 0.7, the rhizospheric Basel Cupric Bacteria-10 agent is obtained.

[0058] Example 4

[0059] This example provides an effect test of rhizosphere Copperbacterium serrata-10 prepared in Example 1, which is to prove that rhizosphere Copperbacterium serrata-10 can promote the growth of Phellodendron amurense seedlings, increase the pigment content of Phellodendron amurense seedlings leaves, regulate the soluble matter content of Phellodendron amurense seedlings leaves and roots, and increase the effective phosphorus content of Phellodendron amurense seedlings leaves, specifically:

[0060] (1) Phellodendron amurense seeds (collected from Hunan Botanical Garden) were sown in 50-hole tree seedling trays, with 5 seeds planted in each hole, and placed in a greenhouse for germination. After the germinated seedlings grew to about 10 cm, they were transplanted.

[0061] (2) Select 10 seedlings with similar growth and transfer them to plastic pots (diameter × height 19.7 cm × 20.5 cm) containing 750 g of nutrient soil, with one plant planted in each pot. The potted seedlings were transferred to the Phellodendron amurense experimental base on the third floor of the School of Life and Environmental Sciences of Central South University of Forestry and Technology for growth. At the same time, attention was paid to watering management. When the Phellodendron amurense seedlings grew to the four-leaf stage (about 40 cm), the rhizosphere inoculation experiment of Basel Copperbacterium-10 was carried out.

[0062] (3) Five of the ten Phellodendron amurense seedlings were randomly selected and inoculated with 25 mL of rhizospheric Cupricobacterium baselense-10 liquid by root irrigation method, which was marked as the CB-10 group. The remaining five Phellodendron amurense seedlings were inoculated with 25 mL of R2A liquid culture medium as the control group, marked as the CK group, and cultured for 40 days. The phenotypes of the Phellodendron amurense seedlings in the CK group and the CB-10 group are shown in Figure 2. Fig.10 .

[0063] 1. Experiment on the growth promotion of rhizospheric copper bacteria-10 in Phellodendron chinense seedlings

[0064] After 40 days of cultivation, the average plant height and ground diameter of the CB-10 group and the CK group were 47.9 cm and 6.80 mm, respectively, and the average plant height and ground diameter of the CK group were 34.6 cm and 4.93 mm, respectively, which were 38.44% and 37.91% higher than those of the CK group (P<0.05), respectively. Figure 6 A and Figure 6 B. The average total fresh weight, leaf fresh weight, stem fresh weight and root fresh weight of P. amurense seedlings in CB-10 group were 62.54 g, 27.52 g, 7.20 g and 21.72 g, respectively. The average total fresh weight, leaf fresh weight, stem fresh weight and root fresh weight of P. amurense seedlings in CK group were 25.98 g, 11.41 g, 3.30 g and 9.21 g, respectively, which were 1.40 times, 1.41 times, 1.18 times and 1.36 times higher than those in CK group, respectively (P<0.05). Figure 6 C- Figure 6 F. At the same time, the average total root length and number of thick roots of the CB-10 group of Phellodendron amurense seedlings were 21.67 cm and 13, respectively, and the average total root length and number of thick roots of the CK group of Phellodendron amurense seedlings were 19.88 cm and 4, respectively, which were 0.09 times and 2.25 times higher than those of the CK group of Phellodendron amurense seedlings, respectively. Figure 6 G and Figure 6 The above results showed that the rhizospheric Cupribotium baselense-10 could promote the growth of Phellodendron amurense seedlings.

[0065] 2. Experiment on the effect of rhizosphere basel copper-grass bacteria-10 on improving the pigment content of leaves of Phellodendron amurense seedlings

[0066] After 40 days of cultivation, the average chlorophyll a, chlorophyll b, carotenoids and total chlorophyll in the leaves of Phellodendron amurense seedlings in the CB-10 group were 6.62 mg / g, 3.55 mg / g, 1.38 mg / g and 10.18 mg / g, respectively, and the average chlorophyll a, chlorophyll b, carotenoids and total chlorophyll in the leaves of Phellodendron amurense seedlings in the CK group were 5.03 mg / g, 2.95 mg / g, 0.74 mg / g and 7.99 mg / g, respectively, which were 31.62%, 20.34%, 87.99% and 27.45% higher than those in the leaves of Phellodendron amurense seedlings in the CK group, and there were significant differences between them and the CK group (P<0.05), see Figure 7 The above results showed that rhizospheric C. thunbergii-10 could increase the pigment content of leaves of P. amurense seedlings, and it was speculated that rhizospheric C. thunbergii-10 promoted the growth of P. amurense seedlings by increasing the content of photosynthetic pigments.

[0067] 3. Experiment on the regulation of soluble substances content in leaves and roots of Phellodendron chinense seedlings by rhizospheric copper bacteria-10

[0068] After 40 days of cultivation, the average soluble sugar contents in the roots and leaves of the CB-10 group and the CK group were 0.15 mg / g and 0.28 mg / g, respectively, and the average soluble sugar contents in the roots and leaves of the CK group were 0.12 mg / g and 0.15 mg / g, respectively, which were 25.00% and 86.67% higher than those in the CK group (P<0.05), respectively. Figure 8 A and Figure 8 B. At the same time, the average fructose content in the roots and leaves of the CB-10 group of Phellodendron amurense seedlings was 48.83μg / g and 63.63μg / g, respectively, and the average fructose content in the roots and leaves of the CK group of Phellodendron amurense seedlings was 37.86μg / g and 67.36μg / g, respectively. The average fructose content in the roots was 28.99% higher than that in the roots of the CK group of Phellodendron amurense seedlings (P<0.05), and the average fructose content in the leaves was 5.58% lower than that in the leaves of the CK group of Phellodendron amurense seedlings, but there was no significant difference between the CK group and the CK group, see Figure 8 C and Figure 8 D. The above results showed that the rhizospheric C. baselense-10 promoted the synthesis and accumulation of soluble sugars in the roots and leaves of P. amurense seedlings, and also promoted the accumulation of fructose in the roots.

[0069] 4. Experiment on increasing the available phosphorus content in leaves of Phellodendron chinense seedlings by using rhizosphere bacteria Baselco-10

[0070] After 40 days of cultivation, the average available phosphorus content in the leaves of Phellodendron amurense seedlings in the CB-10 group and the CK group was 1.29 mg / g, and the average available phosphorus content in the leaves of Phellodendron amurense seedlings in the CK group was 0.79 mg / g, which was 63.72% higher than that in the leaves of Phellodendron amurense seedlings in the CK group (P<0.05). Fig. 9 A. At the same time, the average available phosphorus content in the roots of Phellodendron chinense seedlings in the CB-10 group was 0.31 mg / g, and the average available phosphorus content in the roots of Phellodendron chinense seedlings in the CK group was 0.40 mg / g, which was 22.24% lower than the average available phosphorus content in the roots of Phellodendron chinense seedlings in the CK group, but there was no significant difference between the two groups. Fig. 9 B. The above results showed that the rhizospheric C. baselense-10 significantly promoted the accumulation of available phosphorus in the leaves of P. amurense seedlings, but had no significant effect on the available phosphorus content in the roots.

[0071] Although the principles of the present invention are described in detail above in conjunction with the preferred embodiments of the present invention, those skilled in the art should understand that the above embodiments are merely explanations of the exemplary implementations of the present invention, and are not intended to limit the scope of the present invention. The details in the embodiments do not constitute limitations on the scope of the present invention, and any obvious changes such as equivalent transformations, simple replacements, etc. based on the technical solution of the present invention, without departing from the spirit and scope of the present invention, fall within the protection scope of the present invention.

Claims

1. A rhizosphere copper-greedy bacteria-10, characterized in that The deposit number of the rhizospheric copper-greedy bacteria Basel-10 is GDMCC No: 65501.

2. The rhizosphere Cupricobacterium baselense-10 according to claim 1, characterized in that The nucleotide sequence of the rhizospheric Cupribotium baselense-10 is shown in SEQ ID NO.

1.

3. A rhizosphere copper-greedy bacteria-10 agent, characterized in that: The method is prepared by using the rhizospheric Cupribotium baselense-10 described in claim 1 or 2.

4. A method for preparing the rhizosphere Cupricobacterium Basel-10 bacterial agent according to claim 3, characterized in that: The following steps are involved: The rhizosphere copper-grasping bacteria-10 was inoculated into the culture medium for cultivation. 600 When it is 0.7, the rhizospheric Basel Cupric Bacteria-10 agent is obtained.

5. The method for preparing the rhizosphere Cupricobacterium Basel-10 bacterial agent according to claim 4, characterized in that: The culture temperature is 25-30° C., the culture time is 8-12 h, and the rotation speed is 150-250 rpm; and / or, the culture medium is R2A liquid culture medium.

6. Use of the rhizosphere Cupribotium baselense-10 according to claim 1 or 2 or the rhizosphere Cupribotium baselense-10 agent according to claim 3 in promoting the growth of Phellodendron amurense seedlings.

7. The use according to claim 6, characterized in that: The indexes for promoting the growth of Phellodendron amurense seedlings include plant height, ground diameter, total fresh weight, leaf fresh weight, stem fresh weight, root fresh weight, total root length and number of thick roots of Phellodendron amurense seedlings; The method for promoting the growth of Phellodendron amurense seedlings with the rhizosphere Basel Copperbacterium-10 bacterial agent is as follows: 20-30 mL of the rhizosphere Basel Copperbacterium-10 bacterial agent is inoculated into each Phellodendron amurense seedling grown to the four-leaf stage by the root irrigation method.

8. Use of the rhizosphere Cupribotium baselense-10 according to claim 1 or 2 or the rhizosphere Cupribotium baselense-10 agent according to claim 3 in increasing the pigment content of leaves of Phellodendron amurense seedlings.

9. Use of the rhizosphere Cupriavidus baselense-10 according to claim 1 or 2 or the rhizosphere Cupriavidus baselense-10 agent according to claim 3 in regulating the content of soluble substances in leaves and roots of Phellodendron amurense seedlings.

10. Use of the rhizosphere Cupricobacterium baselense-10 according to claim 1 or 2 or the rhizosphere Cupricobacterium baselense-10 agent according to claim 3 in increasing the content of available phosphorus in leaves of Phellodendron amurense seedlings.

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