A method for virus-free rapid propagation of damask rose

Abstract

The present application relates to the technical field of virus-free rapid propagation of Damask rose, and discloses a virus-free rapid propagation method of Damask rose, which comprises the following steps: a preliminary preparation stage: collecting Damask rose mother plants for pruning pretreatment, and preparing heat treatment equipment, chemical agent solution and materials required for rapid propagation; a virus-free treatment stage: placing the Damask rose mother plants in an artificial climate chamber for heat treatment and virus-free treatment, cutting the Damask rose mother plants into cuttings after the heat treatment and virus-free treatment, and placing the cuttings in the chemical agent solution for chemical agent soaking and virus-free treatment; and a rapid propagation operation stage: soaking the cuttings in a rooting agent for 2-4 hours, and air-drying the cuttings after the soaking. In the present application, the heat treatment and virus-free treatment are combined with the chemical agent virus-free treatment, so that the virus content in Damask rose plants or cuttings can be effectively reduced, and the problem of low yield of good products caused by the single heat treatment and virus-free treatment in the traditional virus-free treatment step can be solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of virus-free rapid propagation of Damask rose, in particular to a virus-free rapid propagation method of Damask rose. BACKGROUND

[0002] Damask rose is a shrub plant of Rosaceae Rosaceae, also known as Bulgarian rose. The plant is about 2 meters high, the small branches have thick and strong hook-shaped thorns, and the small leaves are usually 5 pieces. The flowers are arranged in a 6-12 piece umbrella shape, the flower diameter is 3-5 cm, and the petals are pink. The fruit is pear-shaped or oblate spheroid, red and often has thorn hair. It is suitable for growing in continental climate with distinct seasons, and its flowers can be used for extracting rose essential oil, etc., which has high economic value and ornamental value.

[0003] The traditional virus-free step often uses single heat treatment for virus-free, so it may cause incomplete virus-free, and the virus may easily recur in the subsequent rapid propagation process, thereby causing the problem of low good product rate. SUMMARY

[0004] In view of the defects of the prior art, the present application provides a virus-free rapid propagation method of Damask rose, which solves the problem that the traditional virus-free step often uses single heat treatment for virus-free, which may cause incomplete virus-free, and the virus may easily recur in the subsequent rapid propagation process, thereby causing the problem of low good product rate.

[0005] To achieve the above purpose, the present application realizes the following technical scheme: a virus-free rapid propagation method of Damask rose, comprising the following steps:

[0006] S1, the early preparation stage: collecting Damask rose mother plants for pruning pretreatment, and preparing heat treatment equipment, chemical agent solution and materials required for rapid propagation;

[0007] S2, virus-free treatment stage: placing the Damask rose mother plants in an artificial climate chamber for heat treatment virus-free, cutting the Damask rose mother plants into cuttings after heat treatment virus-free, and placing the cuttings in a chemical agent solution for chemical agent soaking virus-free;

[0008] S3, rapid propagation operation stage: soaking the cuttings in rooting agent for 2-4 hours, air-drying the cuttings after soaking, and then inserting the cuttings into the cutting substrate;

[0009] S4, subsequent management stage: adjusting the temperature, humidity and light conditions of the cutting environment as needed;

[0010] S5, detection stage: detecting the virus of the cut plant.

[0011] Preferably, the Damask rose mother plant in S1 has a straight stem diameter of 0.5-1 cm and a length of 30-50 cm, with a dark green color and smooth surface; the leaves are 5-10 cm long and 3-6 cm wide, with an ovate or ovate-oblong shape, clear and regular serrations on the edges, and no spots, yellowing, or withered parts on the surface of the leaves, nor insect holes or mold spots on the back; the plant is shrub-like, with a height of 1.5-2 meters; the flower branch has 3-5 flowers, with a flower diameter of 3-5 cm and 30-40 petals; the root system is white or light yellow in color, with a main root length of 30-50 cm.

[0012] Preferably, the artificial climate chamber in S2 is set at a temperature of 38-42℃ and a relative humidity of 60-70% for 2-3 weeks.

[0013] Preferably, the cuttings in S2 are 10-15 cm long, and each cutting has 2-3 buds on its outer wall.

[0014] Preferably, the chemical solution in S2 comprises the following raw materials in percentage terms: 0.01%-0.03% triazole nucleoside, 0.015%-0.025% ningnanmycin, 0.25%-0.33% plant disease control agent, and 99.615%-99.725% purified water. The soaking requires that the chemical solution 100% soaks the cuttings, and the soaking is maintained for 1-2 hours. After soaking, the cuttings are rinsed in purified water with a flow rate of 0.5 meters per second for 2-4 minutes.

[0015] Preferably, in step S3, the stalks are placed in a drying machine, with the wind speed controlled at 0.5-1.5 meters per second, the temperature at 20-25°C, the humidity at 40%-60%, and the drying time at 1-3 hours.

[0016] Preferably, in step S3, the cuttings are inserted into the rooting medium to a depth of 1 / 3 to 1 / 2 of the cutting length, and the ambient temperature of the rooting medium is controlled at 20-25°C and the relative humidity is 70-80%.

[0017] Preferably, in step S4, the cutting environment is adjusted as needed. For the first 1-2 weeks after cutting, the light intensity is controlled at 1000-3000 lux, the temperature at 20-25℃, and the relative humidity at 80%-90%. For the next 3-4 weeks, the light intensity is controlled at 3000-5000 lux, the temperature at 22-27℃, and the relative humidity at 70%-80%. After 5 weeks, the light intensity is controlled at 5000-10000 lux, the temperature at 20-28℃, and the relative humidity at 60%-70%.

[0018] This invention provides a rapid propagation method for detoxifying Damask rose. It has the following beneficial effects:

[0019] 1. In this invention, the combination of heat treatment and chemical detoxification can effectively reduce the virus content in Damask rose plants or cuttings. Heat treatment inactivates the virus at high temperatures, while the triazole nucleoside in the chemical agents interferes with viral nucleic acid synthesis, ningnanmycin inhibits viral RNA synthesis, and multiple components of Plant Disease Control Agent exert antiviral effects. This not only improves the quality of propagation materials but also enhances the rooting ability of detoxified cuttings. This improves upon the traditional detoxification process, which often uses a single heat treatment, which may result in incomplete detoxification and easy virus recurrence during subsequent rapid propagation, leading to a low yield of high-quality products.

[0020] 2. In this invention, by controlling the light intensity at 1000-3000 lux, the temperature at 20-25℃, and the relative humidity at 80%-90% for 1-2 weeks after cutting; controlling the light intensity at 3000-5000 lux, the temperature at 22-27℃, and the relative humidity at 70%-80% for 3-4 weeks after cutting; and controlling the light intensity at 5000-10000 lux, the temperature at 20-28℃, and the relative humidity at 60%-70% after 5 weeks of cutting, the success rate and propagation quality of Damask rose cuttings are improved through precise phased control of environmental factors throughout the entire process. Attached Figure Description

[0021] Figure 1 This is a flowchart illustrating the operation of the present invention. Detailed Implementation

[0022] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Please see the appendix Figure 1 This invention provides a method for rapid detoxification and propagation of Damask rose, comprising the following steps:

[0024] S1. Preliminary preparation stage: Collect Damask rose mother plants for pruning and pretreatment, and at the same time prepare heat treatment equipment, chemical solutions and materials required for rapid propagation;

[0025] S2. Detoxification stage: Damask rose mother plants are placed in an artificial climate chamber for heat treatment to detoxify. After heat treatment to detoxify, Damask rose mother plants are cut into cuttings and the cuttings are placed in a chemical solution for chemical soaking to detoxify.

[0026] S3. Rapid propagation stage: Soak the cuttings in rooting agent for 2-4 hours, then air dry the cuttings, and then insert the cuttings into the propagation substrate;

[0027] S4. Subsequent Management Stage: Adjust the temperature, humidity, and light conditions of the cutting environment as needed;

[0028] S5. Testing phase: Virus testing is performed on the plants after cutting.

[0029] Specifically, the cutting substrate is a mixture of vermiculite, perlite, peat moss, and coconut coir in a 2:2:3:3 ratio. The mixture is neither too loose, which would prevent the cuttings from holding together, nor too tight, which would hinder root growth. This allows it to effectively wrap around the cuttings, providing stable support and keeping them upright during rooting. Vermiculite can absorb several times its own weight in water, and the fibrous structure of coconut coir can also absorb and retain a large amount of water. This ensures a continuous water supply to the cuttings, especially in the early stages before rooting when their water requirements are high. Good water retention prevents the cuttings from drying out due to lack of moisture. The porous structure of perlite ensures excellent aeration of the cutting substrate, allowing excess water to drain quickly and preventing waterlogging. The numerous gaps between vermiculite particles further enhance aeration, providing ample oxygen for the respiration process during root development. This promotes healthy root growth and prevents root rot caused by waterlogging. Peat moss, rich in organic matter and nutrients such as nitrogen, phosphorus, and potassium, allows these nutrients to be gradually absorbed and utilized after rooting, providing essential nutritional support for root growth and promoting new shoot development.

[0030] The Damask rose mother plants in S1 have straight stems with a diameter of 0.5-1 cm and a length of 30-50 cm. The stems are dark green and smooth. The leaves are 5-10 cm long and 3-6 cm wide. The leaves are ovate or ovate-oblong in shape with clear and regular serrations on the edges. The leaf surface is free of spots, yellowing, and withered parts, and the underside is free of insect holes and mold. The plant is shrub-like and 1.5-2 meters tall. Each flower branch has 3-5 flowers with a diameter of 3-5 cm and 30-40 petals. The roots are white or light yellow and the taproot is 30-50 cm long.

[0031] Specifically, select branches from Damask rose mother plants with a diameter of 0.5-1 cm, a length of 30-50 cm, a dark green color, and a smooth surface for virus-free rapid propagation. Such branches indicate a robust mother plant with abundant internal nutrients, providing sufficient energy for virus-free treatment and rapid propagation. The dark green color signifies high chlorophyll content and strong photosynthesis, providing a good foundation for subsequent growth. The smooth surface indicates healthy branches free from pests and diseases, reducing interference factors during the virus-free process, improving the success rate of virus-free propagation and the quality of rapid propagation, which is beneficial for cultivating... To cultivate healthy, high-quality Damask rose plants, select leaves of moderate size and regular shape. This indicates a healthy mother plant with normal physiological function. Clear and regular serrations on the leaves demonstrate good plant development, which is conducive to photosynthesis and gas exchange. Leaves should be free of spots, yellowing, and wilting. Selecting leaves without insect holes or mold on the underside ensures the mother plant is free from pests and diseases, reducing interference during the virus-free process and further improving the success rate. Such leaves can better perform photosynthesis, providing sufficient energy for plant growth and subsequent rapid propagation. Large-scale planting lays a solid foundation. The shrub-like growth habit, with a height of 1.5-2 meters, indicates healthy growth and a stable growth structure. Each flower branch bears 3-5 flowers, each 3-5 cm in diameter with 30-40 petals, demonstrating normal reproductive growth and showcasing superior varietal characteristics. This indicates that the mother plant is in good condition regarding nutrient supply and hormone regulation, providing healthy and high-quality material for rapid virus-free propagation. This facilitates the cultivation of virus-free plants with excellent growth and flowering performance, ultimately leading to the propagation of high-quality Damask roses. Seedlings provide protection; white or light yellow roots usually indicate healthy and vigorous roots that can efficiently absorb water and nutrients, providing sufficient nutritional support for the mother plant and subsequent rapid propagation; selecting a taproot length of 30-50 cm ensures that the mother plant is firmly rooted in the soil, enhancing its resistance to lodging, and also reflects the root system's strong ability to explore and absorb soil resources, thus allowing the mother plant to accumulate sufficient nutrient reserves during growth, laying a good foundation for plant growth and development during the virus-free rapid propagation process, and further improving the success rate of virus-free propagation and the quality of rapid propagation.

[0032] The artificial climate chamber in S2 is set at a temperature of 38-42℃ and a relative humidity of 60-70% for 2-3 weeks. The cuttings in S2 are 10-15 cm long, with 2-3 buds on the outer wall of each cutting. The chemical solution in S2 contains the following ingredients in percentage: 0.01%-0.03% triazole nucleoside, 0.015%-0.025% ningnanmycin, 0.25%-0.33% plant disease control agent, and 99.615%-99.725% purified water. The soaking process requires the chemical solution to 100% immerse the cuttings for 1-2 hours. After soaking, rinse with purified water at a flow rate of 0.5 m / s for 2-4 minutes.

[0033] Specifically, setting the temperature in the artificial climate chamber to 38-42℃ and the relative humidity to 60-70% helps inhibit viral activity while avoiding excessive damage to the plants. Cuttings 10-15 cm in length store sufficient nutrients for early rooting. Each cutting has 2-3 buds on its outer wall that produce auxin, promoting cell division and differentiation at the base of the cutting, inducing adventitious root formation, and increasing the rooting rate. Triazole nucleoside interferes with viral nucleic acid synthesis, ningnanmycin inhibits viral RNA synthesis, and plant disease control agent exerts antiviral effects from multiple perspectives. After soaking, rinsing in purified water at a flow rate of 0.5 m / s for 2-4 minutes prevents residual chemicals from unnecessarily affecting subsequent rapid propagation. Combining heat treatment and subsequent chemical treatment in the artificial climate chamber maximizes the detoxification effect, resulting in faster subsequent rapid propagation.

[0034] In S3, the cuttings are placed in a drying machine with the air speed controlled at 0.5-1.5 meters per second, the temperature at 20-25℃, and the humidity at 40%-60%. The drying time is 1-3 hours. In S3, the cuttings are inserted into the rooting medium to a depth of 1 / 3-1 / 2 of their length. The ambient temperature of the rooting medium is controlled at 20-25℃, and the relative humidity is 70-80%.

[0035] Specifically, by placing the cuttings in a desiccant with a wind speed of 0.5-1.5 meters per second, a temperature of 20-25℃, and a humidity of 40%-60%, the cuttings can maintain their physiological activity, reducing the risk of pathogen growth. A desiccant drying time of 1-3 hours ensures the cuttings are in a suitable condition for insertion into the rooting medium, improving the survival rate and rooting quality, thus providing a strong guarantee for the rapid propagation of Damask roses. Inserting the cuttings to a depth of 1 / 3-1 / 2 of their length promotes stable rooting and ensures normal bud growth and development. Maintaining a temperature of 20-25℃ sustains normal physiological activity, promoting cell division and growth. Controlling the relative humidity to 70-80% provides a suitable moisture environment, reducing water evaporation and the risk of drying out, aiding wound healing and rooting, and improving the survival rate and rapid propagation effect, creating favorable conditions for the rapid propagation of Damask roses.

[0036] In S4, adjust the cutting environment as needed. For the first 1-2 weeks after cutting, control the light intensity at 1000-3000 lux, the temperature at 20-25℃, and the relative humidity at 80%-90%. For the next 3-4 weeks, control the light intensity at 3000-5000 lux, the temperature at 22-27℃, and the relative humidity at 70%-80%. After 5 weeks, control the light intensity at 5000-10000 lux, the temperature at 20-28℃, and the relative humidity at 60%-70%.

[0037] Specifically, controlling the light intensity at 1000-3000 lux, maintaining a temperature of 20-25℃, and a relative humidity of 80%-90% for 1-2 weeks after cutting helps the cuttings heal and root, preventing them from drying out and creating a mild environment for the initial rooting stage. Controlling the light intensity at 3000-5000 lux, maintaining a temperature of 22-27℃, and a relative humidity of 70%-80% for 3-4 weeks after cutting promotes new root growth and shoot sprouting, enhancing photosynthesis and nutrient absorption. Controlling the light intensity at 5000-10000 lux, maintaining a temperature of 20-28℃, and a relative humidity of 60%-70% after 5 weeks of cutting allows the cuttings to adapt to the natural environment, resulting in stronger plants and preparing them for transplanting. This allows for precise control of environmental factors at different stages, thereby improving the survival rate and propagation quality of Damask roses.

[0038] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for rapid propagation of Damask rose to eliminate viruses, characterized by: Includes the following steps: S1. Preliminary Preparation Stage: Collect Damask rose mother plants for pruning and pre-treatment, and prepare heat treatment equipment, chemical solutions, and materials needed for rapid propagation. The Damask rose mother plants in S1 have stems with a diameter of 0.5-1 cm and a length of 30-50 cm. The stems are dark green with a smooth surface. The leaves are 5-10 cm long and 3-6 cm wide, with an ovate or ovate-oblong shape, clear and regular serrations on the edges, and no spots, yellowing, or withered parts on the surface, nor insect holes or mold on the underside. The plant is shrub-like, 1.5-2 meters tall. Each flower branch has 3-5 flowers, with a flower diameter of 3-5 cm and 30-40 petals. The roots are white or light yellow, with a taproot length of 30-50 cm. S2. Detoxification Stage: The Damask rose mother plants are placed in an artificial climate chamber for heat treatment to detoxify them. After heat treatment, the Damask rose mother plants are cut into cuttings, which are then immersed in a chemical solution for further detoxification. The artificial climate chamber in S2 is set at a temperature of 38-42℃ and a relative humidity of 60-70% for 2-3 weeks. The cuttings in S2 are 10-15 cm long, with 2-3 buds on the outer wall of each cutting. The chemical solution in S2 comprises the following percentages: 0.01%-0.03% triazole nucleoside, 0.015%-0.025% ningnanmycin, 0.25%-0.33% plant disease control agent, and 99.615%-99.725% purified water. The immersion process requires the chemical solution to completely cover the cuttings for 1-2 hours. After immersion, the cuttings are rinsed in purified water at a flow rate of 0.5 m / s for 2-4 minutes. S3. Rapid propagation stage: Soak the cuttings in rooting agent for 2-4 hours, then air-dry them before inserting them into the propagation substrate. In S3, the air-drying process involves placing the cuttings in a drying machine, controlling the airflow speed at 0.5-1.5 meters per second, the temperature at 20-25℃, and the humidity at 40%-60%, for 1-3 hours. In S3, the cuttings are inserted into the propagation substrate to a depth of 1 / 3-1 / 2 of their length. The ambient temperature of the propagation substrate is controlled at 20-25℃, and the relative humidity at 70-80%. S4. Subsequent Management Stage: Adjust the temperature, humidity, and light conditions of the cutting environment as needed; In S4, the adjustment of the cutting environment as needed involves controlling the light intensity at 1000-3000 lux, maintaining the temperature at 20-25℃, and the relative humidity at 80%-90% for 1-2 weeks after cutting; controlling the light intensity at 3000-5000 lux, maintaining the temperature at 22-27℃, and the relative humidity at 70%-80% for 3-4 weeks after cutting; and controlling the light intensity at 5000-10000 lux, maintaining the temperature at 20-28℃, and the relative humidity at 60%-70% after 5 weeks of cutting. S5. Testing phase: Virus testing is performed on the plants after cutting.

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