A pesticide composition and its application

Abstract

The present invention belongs to the field of pesticide sterilization and discloses a pesticide composition and its use. The active ingredients of the pesticide composition include active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: active ingredient B is either pentoxafen or pentoxafen, and the mass ratio of active ingredient A to active ingredient B is 1:35 to 35:1. The pesticide composition of the present invention has a broad-spectrum fungicidal effect, effectively reduces the amount and cost of the active ingredients used, and delays the development and progression of resistance.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticide sterilization, and in particular to a pesticide composition and application thereof. Background Art

[0002] The compound of formula I is a methoxyacrylate fungicide developed by Sumitomo Chemical Co., Ltd., CAS registration number: 2454319-63-0, Chinese chemical name: (2Z)-3-methoxy-2-[(4-methyl[1,1'-biphenyl]-3-yl)oxy]prop-2-enoic acid methyl ester, and the chemical structure is as follows:

[0003]

[0004] Sedaxane, ISO generic name: sedaxane, is a fungicide belonging to the SDHI inhibitor class. It acts on succinate dehydrogenase, a key enzyme in bacteria that connects oxidative phosphorylation and electron transfer, disrupting the tricarboxylic acid cycle and hindering energy metabolism. This in turn inhibits pathogen growth and leads to its death, thereby preventing and controlling diseases. Sedaxane penetrates from seeds into the surrounding soil, forming a protective barrier around seeds, roots, and stem bases. It exhibits excellent mobility across various soil types, allowing for even distribution across crop roots.

[0005] Penflufen, ISO common name: penflufen, chemical name: N-[2-(1,3-dimethylbutyl)phenyl]-5-1,3-dimethyl-1H-pyrazole-4-carboxamide, CAS registration number: 494793-67-8. Penflufen is a pyrazoleamide fungicide developed by Bayer with excellent activity against a variety of plant pathogenic fungi. It is a succinate dehydrogenase inhibitor, primarily acting at electron transport complex II of the respiratory chain, blocking energy metabolism. Penflufen has systemic, preventive, and therapeutic effects, with a long-lasting effect. It is primarily used as a fungicidal seed treatment agent. After seed treatment, the agent penetrates into germinating seeds and is transported through the xylem of young plants to the entire plant, thereby protecting the growing seedling.

[0006] Long-term use of a single agent and irrational application methods can easily lead to drug resistance in pathogens, resulting in decreased efficacy and disease outbreaks. This in turn increases drug dosage, creating a vicious cycle that increases drug costs and exacerbates environmental pollution. The present invention rationally compounds a compound of Formula I with pentozolin and pentozolinone to analyze the toxicity of different fungicide combinations against wheat sheath blight and corn head smut. Field trials comparing the efficacy of different treatments are also conducted, aiming to provide a theoretical basis for the chemical control of wheat sheath blight and corn head smut. Summary of the Invention

[0007] Based on the above situation, the purpose of the present invention is to provide a pesticide composition and its preparation, which are mainly used to prevent and control plant fungal diseases. The composition or its preparation can enhance the efficacy, reduce the dosage, and at the same time prolong the lasting effect and delay the development of drug resistance.

[0008] In order to achieve the above object, the present invention adopts the following technical solution: a pesticide composition, the pesticide composition comprising active ingredient A and active ingredient B, the active ingredient A is a compound of formula I: The active ingredient B is any one of penflufenacil and penflufenacil; the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 35:1, or any value within the above numerical range;

[0009] Furthermore, the active ingredient B is penflufenacil, and the mass ratio of the active ingredient A to the active ingredient B is 1:22 to 25:1, or any value within the above numerical range;

[0010] The active ingredient B is pentocyclanil, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 20:1, or any value within the above numerical range.

[0011] Furthermore, the active ingredient B is penflufenacil, and the mass ratio of the active ingredient A to the active ingredient B is

[0012] 1:15 to 18:1, or any value within the above range;

[0013] The active ingredient B is pentocyclamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:1, or any value within the above numerical range;

[0014] Furthermore, the active ingredient B is penflufenacil, and the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 15:1, or any value within the above numerical range;

[0015] The active ingredient B is pentocyclamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:16 to 18:1, or any value within the above numerical range;

[0016] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.1 to 95 wt%, preferably 2 to 80 wt%.

[0017] Furthermore, the pesticide composition also includes an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier.

[0018] Furthermore, the pesticide composition is in the form of any one of emulsifiable concentrate, aqueous emulsion, microemulsion, suspension concentrate, wettable powder, seed treatment suspension concentrate or water dispersible granules;

[0019] Furthermore, the formulation of the pesticide composition is a seed treatment suspension.

[0020] The present invention also discloses the use of the above pesticide composition in preventing and controlling plant diseases;

[0021] Furthermore, the plant disease is a wheat disease, rice disease, corn disease or potato disease caused by fungi.

[0022] Furthermore, the diseases are potato black mole, wheat loose smut, wheat sheath blight, wheat head blight, corn leaf spot, corn smut, corn head smut, corn sheath blight, rice sheath blight, rice seedling rot, rice seedling rot, and rice damping-off.

[0023] Furthermore, the diseases are wheat sheath blight and corn head smut.

[0024] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0025] (1) The pesticide composition of the present invention combines the compound of formula I with different mechanisms of action with either penflufenacil or penflufenacil, thereby increasing the fungicidal activity and expanding the scope of application;

[0026] (2) The pesticide composition of the present invention is safe and environmentally friendly, has broad development prospects, and can be applied once during the entire growth period of crops, thereby reducing the amount of pesticide used and lowering agricultural costs;

[0027] (3) The pesticide composition of the present invention has significant preventive effect, a long lasting effect, a broad bactericidal spectrum, and can delay the development of drug resistance in harmful bacteria. DETAILED DESCRIPTION

[0028] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0029] Preparation method of seed treatment suspension: Preparation method of seed treatment suspension: According to the formula ratio, the active ingredients, adjuvants and water are mixed and stirred evenly through high shear, and then sanded by a sander for 2.5 hours to make the average particle size reach 1-5 microns. Finally, thickeners, preservatives and film-forming agents are added and continued to be sheared and stirred evenly to obtain the seed treatment suspension.

[0030] Preparation Example 1: 24% Formula I compound·flufenacil seed treatment suspension concentrate (1:7)

[0031] Calculated by weight percentage, 3% compound of formula I, 21% fluopicolide, 5% fatty amine polyoxyethylene ether phosphate, 2% fatty alcohol polyoxyethylene ether, 2% fatty alcohol ethylene oxide-propylene oxide copolymer, 3% polycarboxylic acid sodium salt, 1% magnesium aluminum silicate, 0.25% xanthan gum, 2% polyacrylic acid emulsion, 5% rose red pigment, 5% ethylene glycol, 0.5% silicone defoamer, 0.5% sodium benzoate, and deionized water to make up the balance.

[0032] Preparation Example 2: 21% Formula I compound·flufenacil seed treatment suspension concentrate (1:2)

[0033] Calculated by weight percentage, 7% compound of formula I, 14% fluopicolide, 2% castor oil polyoxyethylene ether, 1% sodium lignin sulfonate, 5% isotridecyl alcohol polyoxyethylene ether, 2% styrenated phenol polyoxyethylene ether sulfate, 1% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 5% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.15% benzisothiazolinone potassium, and deionized water to make up the balance.

[0034] Preparation Example 3: 30% Formula I compound·flufenacil seed treatment suspension concentrate (5:1)

[0035] Calculated by weight percentage, 25% of the compound of formula I, 5% of fluopicolide, 1% of sorbitan oleate polyoxyethylene ether, 1% of arylphenol polyoxyethylene ether phosphate, 2% of sodium lauryl sulfate, 1% of polyacrylic acid emulsion, 0.25% of xanthan gum, 6% of rose red pigment, 5% of ethylene glycol, 1% of magnesium aluminum silicate, 0.5% of silicone defoamer, 1% of sodium sorbate, and deionized water to make up the balance.

[0036] Preparation Example 4: 25% Formula I compound·flufenacil seed treatment suspension concentrate (2:3)

[0037] Calculated by weight percentage, the following ingredients include: 10% compound of formula I, 15% pentoxazolin, 2% polyoxyethylene sorbitan monooleate, 5% EO / PO block copolymer, 1% phenylethylphenol polyoxyethylene phosphate, 1% naphthalenesulfonate formaldehyde condensate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 6% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.5% isothiazolinone, and deionized water to make up the balance.

[0038] Preparation Example 5: 18% Formula I compound·flufenacil seed treatment suspension concentrate (8:1)

[0039] The composition comprises, by weight, 16% of a compound of formula I, 2% of penflufenamic acid anilide, 3% of an alkylphenol formaldehyde resin polyoxyethylene ether, 3% of an EO / PO block copolymer, 2% of a Guerbet alcohol polyoxyethylene ether, 2% of a naphthalenesulfonate formaldehyde condensate, 1% of polyethylene glycol, 0.25% of xanthan gum, 1% of a polyacrylic acid emulsion, 4% of a rose red pigment, 5% of ethylene glycol, 1% of a magnesium aluminum silicate, 0.5% of a silicone defoamer, 0.2% of a potassium benzoate, and deionized water to make up the balance.

[0040] Preparation Example 6: 22% Formula I compound·fluzoxazone seed treatment suspension concentrate (10:1)

[0041] Calculated by weight percentage, 20% compound of formula I, 2% fluazifop, 3% alkylphenol polyoxyethylene ether, 2% fatty alcohol polyoxyethylene ether sulfate, 0.5% sodium lignin sulfonate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose red pigment, 5% glycerol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% potassium benzisothiazolinone, and deionized water to make up the balance.

[0042] Preparation Example 7: 27% Formula I compound·fluazifop seed treatment suspension concentrate (2:1)

[0043] By weight percentage, 18% of the compound of formula I, 9% fluazifop, 3% castor oil polyoxyethylene ether, 3% alkylphenol polyoxyethylene ether, 1% styrenated phenol polyoxyethylene ether phosphate, 2% sodium alginate, 2% sodium lauryl sulfate, 1% sodium carboxymethyl starch, 0.1% xanthan gum, 5% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium sorbate, and deionized water make up the balance.

[0044] Preparation Example 8: 36% Formula I compound·fluazifop-β-cyclam seed treatment suspension concentrate (1:8)

[0045] By weight percentage, 4% compound of formula I, 32% fluazifop, 3% EO / PO block copolymer, 2% fatty alcohol polyoxyethylene ether sulfate, 2% fatty alcohol polyoxyethylene ether, 1% polyacrylic acid emulsion, 0.2% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium p-hydroxybenzoate, and deionized water to make up the balance;

[0046] Preparation Example 9: 30% Formula I compound·fluazifop seed treatment suspension (5:1)

[0047] By weight percentage, 25% of the compound of formula I, 5% fluazifop, 5% ethylene glycol oxyethylene polyoxypropylene ether, 2% isotridecyl alcohol polyoxyethylene ether, 1% sodium lignin sulfonate, 3% tristyrylphenol ethoxylate phosphate, 1% sodium lignin sulfonate, 2% polyvinyl alcohol, 0.25% xanthan gum, 5% rose red pigment, 5% glycerol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 2% kason, and deionized water make up the balance.

[0048] Preparation Example 10: 14% Formula I compound·fluzoxazone seed treatment suspension concentrate (2:5)

[0049] Calculated by weight percentage, 4% compound of formula I, 10% fluazifop, 3% isomeric tridecyl alcohol polyoxyethylene ether, 3% tristyrylphenol ethoxylate phosphate, 1% sodium alkyl polyoxyethylene ether sulfonate, 1% polyacrylic acid emulsion, 0.3% xanthan gum, 5% rose red pigment, 5% glycerol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0050] Indoor toxicity test

[0051] Example 1: Indoor joint action test of a mixture of the compound of formula I with pentozolin and pentozolin against wheat sheath blight

[0052] Test target: Rhizoctonia cerealis.

[0053] Preparation of test materials: Transfer the pathogens stored in the refrigerator to PDA culture medium plates and culture them at 28℃ for 2 days before use.

[0054] Test agents: technical of the compound of formula I, technical of pentoxapro, and technical of pentoxapro.

[0055] Preparation of pharmaceutical mother solutions: Dissolve the original drugs in appropriate solvents to prepare high-concentration mother solutions, and then dilute the above single agents and mixtures into 5 series of mass concentrations with 0.1% Tween 80 aqueous solution.

[0056] Test repetition: 4 culture dishes were used for each concentration of the test drug, 1 culture dish was used for each repetition, and a total of 4 repetitions were performed. A 0.1% Tween 80 aqueous solution without drug was used as a blank control.

[0057] Drug Treatment: Under aseptic conditions, add pre-thawed sterile culture medium to a sterile conical flask according to the test treatment. Aspirate the drug solution in increments of 100 mg / mL, starting from low to high concentration, and add it to each conical flask. Shake thoroughly. Then, pour equal amounts of the culture medium into four 9 cm diameter plates to create drug-containing plates of the corresponding concentrations. A treatment without drug serves as a blank control. Repeat each treatment four times.

[0058] Inoculation: Aseptically cut a cake of Rhizoctonia graminearum from the edge of a pre-cultured colony using a sterile borer. Then, inoculate the cake onto the center of the drug-containing plate using an inoculator, with the mycelium facing upwards, and cover with a cap. After inoculation, incubate the plate at 28°C.

[0059] Investigation: Investigate the growth of pathogenic mycelia based on the growth of colonies in the blank control culture dish. Measure the diameter of the colonies in centimeters using a caliper. Measure the diameter of each colony once vertically using the cross-hatch method and take the average value.

[0060] Data statistics and analysis: Based on the survey results, calculate the mycelial growth inhibition rate of each treatment concentration on the target bacteria, the unit is percentage (%), and the calculation results are rounded to two decimal places.

[0061] D=D1-D2

[0062] Where:

[0063] D——colony growth diameter;

[0064] D1 - colony diameter;

[0065] D2——diameter of mushroom cake.

[0066]

[0067] Where:

[0068] I——hyphae growth inhibition rate;

[0069] D0——blank control colony growth diameter;

[0070] D T ——The growth diameter of the colony after treatment with chemicals.

[0071] Analyze with statistical analysis system to obtain the toxicity regression line and EC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0072] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of CTC ≥ 120 indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; and 80 < CTC < 120 indicates an additive effect.

[0073] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0074]

[0075] Where:

[0076] ATI - measured toxicity index of mixture;

[0077] S——EC of standard agent 50 , the unit is milligrams per liter (mg / L);

[0078] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0079] TTI=TI A ×P A +TI B ×P B

[0080] Where:

[0081] TTI – Theoretical Toxicity Index of Mixtures;

[0082] TI A ——Agent toxicity index;

[0083] P A ——The percentage of agent A in the mixture, in percentage (%);

[0084] TI B ——Toxicity index of agent B;

[0085] P B ——The percentage of agent B in the mixture, in percentage (%).

[0086]

[0087] Where:

[0088] CTC – Co-toxicity coefficient;

[0089] ATI - measured toxicity index of mixture;

[0090] TTI - Theoretical Toxicity Index of Mixture.

[0091] The test results are shown in the table below:

[0092] Test results:

[0093] Table 1: Results of indoor combined action test on wheat sheath blight of a mixture of a compound of formula I and pentozolinone

[0094]

[0095] Table 2: Results of indoor combined effect test of compound of formula I and oxazolidinone on wheat sharp eye spot

[0096]

[0097]

[0098] It can be seen from the indoor activity tests in Tables 1 and 2 that the compound of formula I in combination with either penflufenacil or penflufenacil exhibited a good inhibitory effect on the mycelial growth of wheat sheath blight pathogen.

[0099] From the test results in Table 1, it can be seen that the compound of formula I and pentozolin have a good control effect on wheat sheath blight pathogens. 50 The co-toxicity coefficients of the compound of formula I and penflufenoxam in the mass ratio range of 1:22 to 25:1 were greater than 120, showing a synergistic effect against wheat sheath blight. The co-toxicity coefficients of the compound of formula I and penflufenoxam in the mass ratio range of 1:14 to 8:1 were greater than 130, showing a significant synergistic effect against wheat sheath blight. The co-toxicity coefficient was highest when the compound of formula I and penflufenoxam were combined in a mass ratio of 1:7, resulting in the most significant synergistic effect.

[0100] From the test results in Table 2, it can be seen that the compound of formula I and fluazifop-butyl have a good control effect on wheat sheath blight pathogens. 50 The co-toxicity coefficients of the compound of formula I and oxazolidinone in the mass ratio range of 1:35 to 20:1 were greater than 120, showing a synergistic effect against wheat sheath blight. The co-toxicity coefficients of the compound of formula I and oxazolidinone in the mass ratio range of 1:25 to 18:1 were greater than 130, showing a significant synergistic effect against wheat sheath blight. Among them, the co-toxicity coefficient was the highest when the compound of formula I and oxazolidinone were compounded in a mass ratio of 2:1, and the synergistic effect was the most significant.

[0101] Example 2: Indoor joint action test of a mixture of the compound of formula I with penflufenacil and penflufenacil against corn head smut

[0102] Test agents: technical of the compound of formula I, technical of pentoxapro, and technical of pentoxapro.

[0103] Test pathogen: Maize head smut pathogen (Sphacelotheca reiliana).

[0104] Preparation of pharmaceutical preparations: Dissolve the above raw drugs in a suitable solvent and then dilute with 0.1% Tween 80 aqueous solution; prepare single-dose stock solutions respectively, and set a series of mass concentrations according to the mixing purpose and pharmaceutical activity.

[0105] Preparation of spore suspension: Weigh 3 mg of powder of head smut fungus, add 15 ml of 1% CuSO4 solution and soak for 24 hours, then centrifuge at 4000 r / min for 5 minutes, discard the supernatant, add sterile water, shake well and centrifuge again, and prepare a spore suspension with sterile water after precipitating the spores. The suspension should be adjusted to about 50 spores per field of view for observation under a low-power microscope (10×20).

[0106] Medium Preparation: Melt PDA medium in a microwave oven, cool to approximately 50°C, and add 1 mL of the prepared test solution and 9 mL of PDA medium to a 9 cm diameter Petri dish, working from low to high concentration. Mix thoroughly to prepare drug-containing plates of the appropriate concentration. Repeat four times for each treatment.

[0107] Inoculation and Incubation: 0.1 ml of spore suspension was added to each treatment on a prepared PDA plate. The plate was then scraped and evenly spread. The plate was then placed in a 28°C incubator. After 72 hours of incubation, when the control germination rate reached its maximum, spore germination was observed for each treatment. A total of 300 spores were examined in three fields per PDA plate.

[0108] Result calculation:

[0109]

[0110] Statistical analysis: Regression analysis was performed based on the logarithmic values ​​of each agent concentration and the corresponding probability values ​​of spore germination inhibition rate to calculate the EC of each agent. 50 Equivalence was used to evaluate the activity of the test agent on the biological test material.

[0111] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of CTC ≥ 120 indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; and 80 < CTC < 120 indicates an additive effect.

[0112] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0113]

[0114] Where:

[0115] ATI - measured toxicity index of mixture;

[0116] S——EC of standard agent 50 , the unit is milligrams per liter (mg / L);

[0117] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0118] TTI=TI A ×PA +TI B ×P B

[0119] Where:

[0120] TTI – Theoretical Toxicity Index of Mixtures;

[0121] TI A ——Agent toxicity index;

[0122] P A ——The percentage of agent A in the mixture, in percentage (%);

[0123] TI B ——Toxicity index of agent B;

[0124] P B ——The percentage of agent B in the mixture, in percentage (%).

[0125]

[0126] Where:

[0127] CTC – Co-toxicity coefficient;

[0128] ATI - measured toxicity index of mixture;

[0129] TTI - Theoretical Toxicity Index of Mixture.

[0130] The test results are shown in the table below:

[0131] Table 3: Results of indoor combined effect test on corn head smut of a mixture of compound of formula I and penflufenacil

[0132]

[0133] Table 4: Indoor joint effect test of the mixture of compound of formula I and pentozolin against corn head smut

[0134]

[0135] It can be seen from the indoor activity tests in Tables 3 and 4 that the compound of formula I in combination with either penflufenacil or penflufenacil exhibited a good inhibitory effect on the spore germination of corn head smut.

[0136] From the test results in Table 3, it can be seen that the compound of formula I and penflufenacil have a good control effect on corn head smut pathogens. 50The co-toxicity coefficients of the compound of formula I and penflufenoxam in the mass ratio range of 1:22 to 25:1 were greater than 120, showing a synergistic effect against corn head smut. The co-toxicity coefficients of the compound of formula I and penflufenoxam in the mass ratio range of 1:15 to 18:1 were greater than 130, showing a significant synergistic effect against corn head smut. The co-toxicity coefficient was highest when the compound of formula I and penflufenoxam were combined in a mass ratio of 2:3, resulting in the most significant synergistic effect.

[0137] From the test results in Table 4, it can be seen that the compound of formula I and pentocyclam have a good control effect on corn head smut pathogens. 50 The co-toxicity coefficients of the compound of formula I and pentocyclam in the mass ratio range of 1:30 to 20:1 were greater than 120, showing a synergistic effect against corn head smut. The co-toxicity coefficients of the compound of formula I and pentocyclam in the mass ratio range of 1:10 to 10:1 were greater than 130, showing a significant synergistic effect against corn head smut. The co-toxicity coefficient was the highest when the compound of formula I and pentocyclam were combined in a mass ratio of 2:5, and the synergistic effect was most significant.

[0138] Example 3: Field efficacy test for controlling wheat sheath blight

[0139] The trial was conducted in Baimiao Village, Gebukou Township, Yuanyang County, Henan Province, from wheat sowing to harvest. Cultivation and management conditions were uniform across the experimental area. Wheat sheath blight has been widespread and uniform over the years.

[0140] Test target: Wheat sheath blight.

[0141] Experimental crop: wheat (Yumai 49).

[0142] Experimental Design: One day before sowing, wheat seeds were treated with the test agent using water according to the dosage in the table below and allowed to air dry. A clear water control was provided. Each treatment was replicated four times in a randomized block arrangement. Sowing was conducted using a manual seeder on the morning of October 18th.

[0143] Experimental investigation: The incidence of wheat sheath blight was investigated during the jointing stage. Samples were taken at five fixed points along the diagonal of each plot, with 100 plants sampled at each point. The disease index and control efficacy were calculated using the following grading method.

[0144] Grading standards:

[0145] Level 0, no disease;

[0146] Level 1, leaf sheaths are diseased but stems are not;

[0147] Level 3: The leaf sheath is infected and invades the stem, but the lesions on the stem are less than 1 / 2 of the stem;

[0148] Level 5: The lesions on the stems encircle more than 1 / 2 of the stems, but the stems do not fall over or break;

[0149] Level 7: death, lodging, and dry white ears.

[0150] Yield survey: Yield is measured during the wheat harvest period and the yield increase rate is calculated.

[0151] Calculation method:

[0152]

[0153]

[0154] The results of the field efficacy test are shown below:

[0155] Table 5: Field efficacy test results for wheat sheath blight

[0156]

[0157] As can be seen from Table 5, the compound preparation treatment has an obvious overall prevention and control effect on wheat sheath blight. The disease index of wheat sheath blight in the test plots after treatment is significantly reduced, and the compound preparation also has a good yield-increasing effect on wheat seed treatment.

[0158] Example 4: Field efficacy test for controlling corn head smut

[0159] The experimental site is the experimental field in Zhaizi Village, Xujialou Township, Taishan District, Tai'an City, Shandong Province. The experimental site is sandy loam with medium soil fertility.

[0160] Test target: Corn head smut.

[0161] Experimental crop: corn (Zhengdan 958).

[0162] Experimental design: Each treatment was weighed according to the designed dosage and placed in a plastic bag. The bag was held tightly to fill the bag with air and shaken thoroughly until the seeds were evenly coated. Dry in the shade before sowing. Sowing was carried out on April 26.

[0163] Disease survey: After all ears emerge and symptoms become apparent, investigate corn head smut test results. Randomly survey 500 plants in each plot, record the total number of plants and the number of diseased plants, and calculate the incidence and control effectiveness.

[0164] Yield determination: Harvest corn in the entire plot when mature, dry and thresh it, and weigh it individually to calculate the yield and yield increase rate.

[0165] Calculation method of drug efficacy:

[0166]

[0167]

[0168] The results of the field efficacy test are shown below:

[0169] Table 6: Field test results of efficacy of drugs against corn head smut

[0170]

[0171] As shown in Table 6, the compound suspension seed coating agent had a good control effect on corn head smut at the test dosage, and the yield of each treatment was significantly higher than that of the blank control; the yield increase range was 7.46% to 9.63%.

[0172] Laboratory toxicity assays and field trials demonstrate that the pesticide composition of the present invention exhibits excellent control efficacy against crop diseases, particularly wheat sheath blight and corn head smut. The resulting pesticide composition or formulation exhibits significant control efficacy, outperforming single agents in delaying the development of resistance and prolonging drug retention. Furthermore, no phytotoxicity was observed in the experiments, demonstrating that the resulting pesticide composition or formulation, while enhancing its synergistic bactericidal efficacy, can reduce production and usage costs, while maintaining crop safety.

[0173] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that The pesticide composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula I: (Formula I), the active ingredient B is any one of penflufenacil and penflufenacil; The active ingredient B is penflufenacil, and the mass ratio of the active ingredient A to the active ingredient B is 1:22 to 25:1; The active ingredient B is pentocyclanil, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 20:

1.

2. The pesticide composition according to claim 1, characterized in that The active ingredient B is penflufenacil, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 18:1; The active ingredient B is pentocyclanil, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:

1.

3. The pesticide composition according to claim 2, characterized in that The active ingredient B is penflufenacil, and the mass ratio of the active ingredient A to the active ingredient B is 1:14-15:1; The active ingredient B is pentocyclanil, and the mass ratio of the active ingredient A to the active ingredient B is 1:16-18:

1.

4. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition as 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.1 to 95 wt%.

5. The pesticide composition according to claim 4, characterized in that Based on the total weight of the pesticide composition as 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 2 to 80 wt%.

6. The pesticide composition according to claim 1, characterized in that The pesticide composition further comprises an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier.

7. The pesticide composition according to claim 1, characterized in that The formulation of the pesticide composition is any one of emulsifiable concentrate, aqueous emulsion, microemulsion, suspension, wettable powder, seed treatment suspension or water dispersible granules.

8. The pesticide composition according to claim 7, characterized in that The formulation of the pesticide composition is a seed treatment suspension.

9. Use of the pesticide composition according to any one of claims 1 to 8 in preventing and controlling plant diseases, characterized in that: The diseases are wheat sheath blight and corn head smut.

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