Composition for inhibiting dental plaque and application thereof

Abstract

The invention provides a composition for inhibiting dental plaque and application thereof, and belongs to the technical field of oral care. The composition for inhibiting dental plaque is prepared from the following components in percentage by mass: 0.05 to 0.5 percent of glucan, 0.1 to 1 percent of breast milk oligosaccharide, 0.05 to 0.5 percent of plaque decomposing enzyme, 0.5 to 2 percent of coloring agent, 0.2 to 5 percent of stabilizing agent, 20 to 40 percent of dispersing agent, 0.1 to 2 percent of flavoring agent and the balance of water. The oral care product prepared from the composition can have good plaque dyeing performance, good antibacterial effect and good stability at the same time.

Description

Technical Field

[0001] The present application relates to the technical field of oral care, and in particular to a composition for inhibiting dental plaque and an application thereof. Background Art

[0002] Dental plaque is the main cause of a series of periodontal diseases such as bad breath, gingivitis, and caries. In order to effectively inhibit dental plaque, some oral care products are usually used. However, current oral care products are difficult to have good plaque staining performance, good antibacterial effect, and good product stability at the same time, resulting in restrictions on the promotion and application of such products. Summary of the invention

[0003] The purpose of the present application is to provide a composition for inhibiting dental plaque and its application, so that the oral care product prepared by the composition can have good plaque staining performance, good antibacterial effect and good stability through a unique formula design.

[0004] The embodiment of the present application is implemented as follows:

[0005] In a first aspect, an embodiment of the present application provides a composition for inhibiting dental plaque, which comprises, by mass percentage: 0.05-0.5% dextran, 0.1-1% human milk oligosaccharides, 0.05-0.5% plaque-decomposing enzyme, 0.5-2% colorant, 0.2-5% stabilizer, 20-40% dispersant, 0.1-2% flavoring agent and the remainder water.

[0006] In the above technical scheme, on the basis of the main active ingredients such as human milk oligosaccharides, plaque-decomposing enzymes and dyes contained in the composition, a specific mass percentage of glucan is further added as an auxiliary ingredient. On the one hand, the molecular weight of glucan is relatively large, and it has a certain thickening and suspending effect in water, so that active ingredients such as dyes can be stably present in the corresponding system to reduce the risk of agglomeration and sedimentation of the active ingredients; on the other hand, glucan is a polysaccharide composed of glucose monomers linked by glycosidic bonds, has good film-forming properties, and has a large number of hydroxyl groups with good hydrophilicity. After it adheres to dental plaque and forms a film, it can adsorb active ingredients onto its film surface to achieve continuous release of active ingredients, so as to prolong the action time of the active ingredients; on the third hand, since dental plaque itself contains a large amount of glucan, and it also enriches glucan itself, when glucan is added as an auxiliary component, it can transport the adsorbed various active ingredients to the plaque in a targeted manner to exert their effects. Therefore, in the present application, by adding glucan as an auxiliary ingredient to the composition, in addition to improving the stability of the composition, the various active ingredients in the composition can be directed to the dental plaque and the continuous release of the active ingredients can be achieved, so that the various active ingredients can better exert their respective effects while also better cooperating with the effects of the remaining active ingredients (specifically, on the one hand, human milk oligosaccharides can have a directional effect on the microorganisms in the oral cavity, especially in dental plaque, thereby promoting the proliferation of beneficial bacteria and inhibiting the growth of harmful bacteria; at the same time, plaque-decomposing enzymes can dissolve bacteria in the oral cavity, especially in dental plaque, and adjust the composition of dental plaque microorganisms, so that they can work in conjunction with human milk oligosaccharides to control dental plaque from a microbial perspective; on the other hand, dye molecules can dye dental plaque in a directional manner, helping to achieve rapid and deep coloring, thereby guiding the removal of dental plaque by mechanical brushing. Through the joint action of the two aspects, dental plaque can be comprehensively controlled from multiple angles), and finally the oral problems caused by dental plaque can be better solved, so that the oral care product prepared by the composition can have good plaque staining performance, good antibacterial effect and good stability at the same time.

[0007] In some alternative embodiments, the glucan comprises β-glucan.

[0008] In the above technical solution, a specific type of β-glucan is used, which is compatible with various active ingredients in the composition, thereby helping to improve the stability, dyeing performance and antibacterial effect of the composition corresponding to the oral care product.

[0009] In some alternative embodiments, the β-glucan is produced by fermentation of Schizophyllum.

[0010] In the above technical scheme, the types of nucleoside bonds in the β-glucans produced by fermentation of different bacterial species are different. Among them, the β-glucan produced by fermentation of Schizophyllum is composed of specific glycosidic bonds of β-(1,3)-(1,6), and the proportion of β-(1,3) glycosidic bonds is not less than 50%, so that the β-glucan has relatively excellent adhesion and permeability, so that it can quickly and specifically bind to bacterial plaques, thereby having the function of better directional and continuous release of various active ingredients.

[0011] In some optional embodiments, the human milk oligosaccharide is selected from at least one of 2'-fucosyllactose, 3-fucosyllactose, lactose-N-neotetraose, 6'-sialyllactose sodium salt and 3'-sialyllactose sodium salt.

[0012] In the above technical solutions, the applicable types of human milk oligosaccharides are relatively rich, and more feasible implementation plans can be provided, thereby facilitating the promotion and application of the technical solutions provided in the embodiments of the present application.

[0013] In some optional embodiments, the plaque decomposing enzyme includes lysozyme, and the enzyme activity of the lysozyme is not less than 3000 U / mg.

[0014] In the above technical solution, lysozyme is used as the plaque decomposing enzyme, and its enzyme activity is limited to a specific range, so as to be able to more effectively and directionally decompose harmful bacteria in the oral cavity that are related to the formation of dental plaque.

[0015] In some optional embodiments, the dye is selected from at least one of basic fuchsin, besmer brown, neutral red, iodine dyes and erythrosine.

[0016] In the above technical solution, the applicable types of dyes are relatively rich, and more feasible implementation plans can be provided, thereby facilitating the promotion and application of the technical solution provided in the embodiment of the present application.

[0017] In some optional embodiments, the stabilizer is selected from at least one of hydrogenated castor oil, poloxamer, Tween and alkylene oxide-polysiloxane polymer.

[0018] In the above technical solution, the applicable types of stabilizers are relatively rich, and more feasible implementation plans can be provided, thereby facilitating the promotion and application of the technical solution provided in the embodiment of the present application.

[0019] In some optional embodiments, the dispersant is selected from at least one of sorbitol, glycerol, polyethylene glycol, propylene glycol, 1,3-butylene glycol, hexylene glycol and ethanol; and / or the flavoring agent is selected from at least one of a sweetener and a flavor.

[0020] In the above technical solutions, the applicable types of dispersants and flavoring agents are relatively rich, and more feasible implementation plans can be provided, thereby facilitating the promotion and application of the technical solutions provided in the embodiments of the present application.

[0021] In a second aspect, an embodiment of the present application provides a use of the composition for inhibiting dental plaque provided in an embodiment of the first aspect in the preparation of an oral care product.

[0022] In the above technical scheme, the composition for inhibiting dental plaque provided by the first embodiment is used in the preparation of oral care products. Since the composition contains dextran as an auxiliary ingredient, the composition has good stability. At the same time, the various active ingredients in the composition can be directed to the dental plaque and the continuous release of the active ingredients can be achieved, so that the various active ingredients can better exert their respective effects while also better cooperating with the effects of the remaining active ingredients, thereby enabling the corresponding oral care products to simultaneously have good plaque staining performance, good antibacterial effect and good stability.

[0023] In some optional embodiments, the dosage form of the oral care product is selected from any one of a paste, a spray, a gel and a solution.

[0024] In the above technical solutions, the dosage forms of oral care products are relatively rich and can be adaptively selected according to actual needs, thereby facilitating the promotion and application of the technical solutions provided in the embodiments of the present application. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0026] It should be noted that “and / or” in this application, such as “Feature 1 and / or Feature 2”, refers to the three situations of “Feature 1” alone, “Feature 2” alone, or “Feature 1” plus “Feature 2”.

[0027] In addition, in the description of the present application, unless otherwise specified, the meaning of "multiple" in "one or more" refers to two or more; the range of "value a ~ value b" includes the two end values ​​"a" and "b", and the "unit" in "value a ~ value b + unit of measurement" represents the "unit of measurement" of both "value a" and "value b".

[0028] The following is a detailed description of a composition for inhibiting dental plaque and its application in an embodiment of the present application.

[0029] In a first aspect, an embodiment of the present application provides a composition for inhibiting dental plaque, which comprises, by mass percentage: 0.05-0.5% dextran, 0.1-1% human milk oligosaccharides, 0.05-0.5% plaque-decomposing enzyme, 0.5-2% colorant, 0.2-5% stabilizer, 20-40% dispersant, 0.1-2% flavoring agent and the remainder water.

[0030] In the present application, on the basis of the main active ingredients such as human milk oligosaccharides, plaque-decomposing enzymes and dyes contained in the composition, a specific mass percentage of glucan is further added as an auxiliary ingredient. On the one hand, the molecular weight of glucan is relatively large, and it has a certain thickening and suspending effect in water, so that active ingredients such as dyes can be stably present in the corresponding system to reduce the risk of agglomeration and sedimentation of the active ingredients; on the other hand, glucan is a polysaccharide composed of glucose monomers linked by glycosidic bonds, has good film-forming properties, and has a large number of hydroxyl groups with good hydrophilicity. After it adheres to dental plaque and forms a film, it can adsorb active ingredients onto its film surface to achieve continuous release of active ingredients, so as to prolong the action time of the active ingredients; on the third hand, since dental plaque itself contains a large amount of glucan, and it also enriches glucan itself, when glucan is added as an auxiliary component, it can transport the adsorbed various active ingredients to the plaque in a targeted manner to exert their effects. Therefore, in the present application, by adding glucan as an auxiliary ingredient to the composition, in addition to improving the stability of the composition, the various active ingredients in the composition can be directed to the dental plaque and the continuous release of the active ingredients can be achieved, so that the various active ingredients can better exert their respective effects while also better cooperating with the effects of the remaining active ingredients (specifically, on the one hand, human milk oligosaccharides can have a directional effect on the microorganisms in the oral cavity, especially in dental plaque, thereby promoting the proliferation of beneficial bacteria and inhibiting the growth of harmful bacteria; at the same time, plaque-decomposing enzymes can dissolve bacteria in the oral cavity, especially in dental plaque, and adjust the composition of dental plaque microorganisms, so that they can work in conjunction with human milk oligosaccharides to control dental plaque from a microbial perspective; on the other hand, dye molecules can dye dental plaque in a directional manner, helping to achieve rapid and deep coloring, thereby guiding the removal of dental plaque by mechanical brushing. Through the joint action of the two aspects, dental plaque can be comprehensively controlled from multiple angles), and finally the oral problems caused by dental plaque can be better solved, so that the oral care product prepared by the composition can have good plaque staining performance, good antibacterial effect and good stability at the same time.

[0031] In the prior art, glucan and human milk oligosaccharides are usually added as prebiotics, and the effects of the two are comparable. However, in the present application, glucan is used as an auxiliary component, mainly to enable the antibacterial effect of human milk oligosaccharides to be better exerted, and to have a synergistic effect with human milk oligosaccharides, and can also usually cooperate with other active ingredients (such as dyes and plaque decomposing enzymes). Specifically, it helps the dye to exist stably for a long time and improve its dyeing effect, and helps to improve the antibacterial effect of plaque decomposing enzymes.

[0032] It should be noted that the mass percentage of dextran is limited to the range of 0.05-0.5%, for example, it can be any point value among 0.05%, 0.1%, 0.2%, 0.3%, 0.4% and 0.5%, or a range value between any two of them. The upper limit of the added amount is set to 0.5% because dextran is a large molecule, and excessive addition makes it difficult to dissolve and disperse well in the composition system, and its effect cannot be better exerted, and the risk of precipitation of the remaining active ingredients may also increase.

[0033] It should be noted that there are many types of glucans, such as α-glucan, β-glucan and yeast glucan. Since glucan needs to have three functions in the composition provided in the present application, the type of glucan can be optimized.

[0034] As an example, the glucan includes β-glucan.

[0035] In this embodiment, a specific type of β-glucan is used, which is compatible with various active ingredients in the composition, thereby helping to improve the stability, dyeing performance and antibacterial effect of the oral care product corresponding to the composition.

[0036] It should be noted that the common glycosidic bonds of β-glucan are mainly of three types: β-(1,3), β-(1,4) and β-(1,6), and the specific composition of β-glucan is determined by the bacterial species. Specifically, cereals can produce β-glucan composed of specific glycosidic bonds of β-(1,3)-(1,4), algae can produce β-glucan composed of specific glycosidic bonds of β-(1,3), and fungi can produce β-glucan composed of specific glycosidic bonds of β-(1,3)-(1,6). Since glucan needs to have three functions at the same time in the composition provided in the present application, the bacterial species type of β-glucan can be optimized to obtain β-glucan that is more suitable for the composition of the present application.

[0037] As an example, beta-glucan is produced by fermentation of Schizophyllum.

[0038] In this embodiment, the β-glucan produced by the fermentation of Schizophyllum is composed of specific glycosidic bonds of β-(1,3)-(1,6), and the proportion of β-(1,3) glycosidic bonds is not less than 50%, so that the β-glucan has relatively excellent adhesion and permeability, so that it can quickly and specifically bind to bacterial plaque, thereby having the function of better directional and sustained release of various active ingredients.

[0039] As an example, the human milk oligosaccharide is at least one selected from 2'-fucosyllactose, 3-fucosyllactose, lactose-N-neotetraose, 6'-sialyllactose sodium salt and 3'-sialyllactose sodium salt.

[0040] It should be noted that human milk oligosaccharides are not easily destroyed by human gastric acid, nor are they easily decomposed by digestive enzymes. They can stimulate the growth of beneficial bacteria in the mouth and intestines (such as bifidobacteria and lactobacilli), thereby indirectly inhibiting the growth of harmful bacteria and maintaining the balance of the oral microecology.

[0041] In this embodiment, the applicable types of human milk oligosaccharides are relatively rich, and more feasible implementation plans can be provided, thereby facilitating the promotion and application of the technical solutions provided in the embodiments of the present application.

[0042] As an example, the plaque decomposing enzyme includes lysozyme, and the enzyme activity of the lysozyme is not less than 3000 U / mg.

[0043] It should be noted that lysozyme, also known as muramidase or N-acetyl muramid polysaccharide hydrolase, is an alkaline enzyme that can hydrolyze mucopolysaccharides in bacteria. Lysozyme mainly breaks down the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, thereby decomposing the insoluble mucopolysaccharides in the cell wall into soluble glycopeptides, causing the cell wall to rupture and the contents to escape, thereby dissolving the bacteria. It is often used as a bactericide and preservative.

[0044] In this embodiment, lysozyme is used as the plaque decomposing enzyme, and its enzyme activity is limited to a specific range, so that harmful bacteria related to the formation of dental plaque in the oral cavity can be more effectively and directionally decomposed.

[0045] It should be noted that dyes are a type of pigment that can dye biofilms such as plaque. Different physical adsorption effects of the tooth surface and plaque on pigments can be used to dye the plaque, visualize the plaque, and guide mechanical methods (such as brushing) to remove the plaque. However, dyes usually have the risk of easy aggregation or even sedimentation in the composition, which affects the stability and dyeing effect of the corresponding product. In this application, the problem of easy aggregation and sedimentation of the dye can be simultaneously improved by adding dextran.

[0046] As an example, the dye is selected from at least one of basic fuchsin, besmer brown, neutral red, iodine dyes and erythrosine.

[0047] In this embodiment, the applicable types of dyes are relatively rich, and more feasible implementation plans can be provided, thereby facilitating the promotion and application of the technical solutions provided in the embodiments of the present application.

[0048] It should be noted that the type of stabilizer is not limited and can be set according to conventional selection in the art.

[0049] As an example, the stabilizer is selected from at least one of hydrogenated castor oil, poloxamer, Tween and alkylene oxide-polysiloxane polymer.

[0050] In this embodiment, the applicable types of stabilizers are relatively rich, and more feasible implementation plans can be provided, thereby facilitating the promotion and application of the technical solutions provided in the embodiments of the present application.

[0051] It should be noted that the types of dispersants and flavoring agents are not limited and can be set according to conventional selection in the art.

[0052] As an example, the dispersant is selected from at least one of sorbitol, glycerol, polyethylene glycol, propylene glycol, 1,3-butylene glycol, hexylene glycol and ethanol; and / or the flavoring agent is selected from at least one of a sweetener and a flavor.

[0053] In this embodiment, the applicable types of dispersants and flavoring agents are relatively rich, and more feasible implementation plans can be provided, thereby facilitating the promotion and application of the technical solutions provided in the embodiments of the present application.

[0054] As an example, the sweetener is selected from at least one of xylitol, sodium saccharin, sucralose, and stevia extract; and the flavor is selected from at least one of peppermint oil, strawberry flavor, and peach flavor.

[0055] It should be noted that the functional components and their mass proportions not specifically described or limited in the composition for inhibiting dental plaque can be set according to conventional selections in the art.

[0056] In a second aspect, an embodiment of the present application provides a use of the composition for inhibiting dental plaque provided in an embodiment of the first aspect in the preparation of an oral care product.

[0057] In the present application, the composition for inhibiting dental plaque provided in the first embodiment is used to prepare oral care products. Since the composition contains dextran as an auxiliary ingredient, the composition has good stability. At the same time, the various active ingredients in the composition can be directed to the dental plaque and the continuous release of the active ingredients can be achieved, so that the various active ingredients can better exert their respective effects while also better cooperating with the effects of the remaining active ingredients, thereby enabling the corresponding oral care products to simultaneously have good plaque staining performance, good antibacterial effect and good stability.

[0058] It should be noted that the preparation process of the oral care product is not limited in the embodiments of the present application, and can be specifically carried out according to the conventional preparation process of the corresponding product in the art.

[0059] As an example, the dosage form of the oral care product is selected from any one of a paste (such as toothpaste), a spray (such as oral spray), a gel and a solution (such as mouthwash).

[0060] In this embodiment, the dosage forms of the oral care product are relatively rich and can be adaptively selected according to actual needs, thereby facilitating the promotion and application of the technical solution provided in the embodiment of the present application.

[0061] The features and performances of the present application are further described in detail below in conjunction with the examples. In the present application, a specific preparation of a coating agent is used for illustration.

[0062] Example 1

[0063] The present invention provides a method for preparing an oral care product (smear), comprising the following steps:

[0064] According to the mass percentage, 0.2% of glucan (the strain is Schizophyllum) is dispersed in a dispersant composed of 30% propylene glycol and 10% ethanol. After it is completely dissolved, 0.5% human milk oligosaccharide (2'-fucosyllactose), 1% erythrosine (dye), and 0.1% lysozyme (plaque decomposing enzyme) are added and mixed evenly to obtain a dispersion.

[0065] 2% of alkylene oxide-polysiloxane polymer (stabilizer) is dissolved in water, and then the above dispersion is added into the water under stirring conditions. Finally, a flavoring agent consisting of 0.5% of xylitol and 0.1% of strawberry flavor is added into the water and stirred and mixed. Finally, the mixture is filtered and filled to obtain an oral care product.

[0066] The subsequent embodiments and comparative examples are carried out with reference to the steps of embodiment 1. In order to better understand the differences in components between the embodiments and comparative examples, the different components are summarized in the form of a table below. For details, please refer to Table 1.

[0067] Table 1

[0068] sample Dextran(%) Glucan strains Human milk oligosaccharides (%) Coloring agent (%) Plaque decomposing enzyme (%) Example 1 0.2 Schizophyllum 2'-fucosyllactose 0.5 Erythrosin 1 Lysozyme 0.1 Example 2 0.5 Schizophyllum 2'-fucosyllactose 0.5 Erythrosin 1 Lysozyme 0.1 Example 3 0.2 Schizophyllum 2'-fucosyllactose 0.5 Erythrosin 2 Lysozyme 0.1 Example 4 0.2 Schizophyllum 2'-fucosyllactose 0.5 Erythrosin 1 Lysozyme 0.5 Comparative Example 1 0 — 2'-fucosyllactose 0.5 Erythrosin 1 Lysozyme 0.1 Comparative Example 2 0.7 Schizophyllum 2'-fucosyllactose 0 Erythrosin 1 Lysozyme 0.1 Comparative Example 3 0 — 2'-fucosyllactose 0.7 Erythrosin 1 Lysozyme 0.1 Comparative Example 4 0.2 Schizophyllum 2'-fucosyllactose 0 Erythrosin 1 Lysozyme 0.1 Comparative Example 5 0.2 Schizophyllum 2'-fucosyllactose 0.5 Erythrosin 1 Lysozyme 0 Comparative Example 6 0.2 Schizophyllum 2'-fucosyllactose 0.5 Erythrosin 0 Lysozyme 0.1

[0069] It should be noted that the differences caused by the adjustment of the dosage of the components in Table 1 were balanced by adjusting the dosage of water.

[0070] Test example

[0071] Performance Testing of Oral Care Products

[0072] Test Method

[0073] The oral care products prepared in Examples 1 to 4 and Comparative Examples 1 to 6 were numbered respectively, and then the stability, antibacterial performance and dyeing performance of each sample were tested.

[0074] 1. The stability test steps are as follows:

[0075] The initial appearance, the appearance after 1 month and the appearance after 3 months of each of the smear samples prepared in Examples 1 to 4 and Comparative Examples 1 to 6 were observed to obtain the stability results of each sample and statistically show them in Table 2.

[0076] Table 2

[0077]

[0078]

[0079] It should be noted that normal appearance means that the solution is clear and transparent with no precipitation at the bottom.

[0080] 2. The test steps for antibacterial performance are as follows, refer to Appendix C4 of the Hygiene Standards for Disposable Hygiene Products:

[0081] (1) Add 75 mL of sterile PBS (0.03 mol / L, pH 7.2-7.4, as a blank control group) and 25 g of an oral care product (the samples in Examples 1-4 and Comparative Examples 1-6 are used as sample solutions) to a 250 mL sterile conical flask containing glass beads, shake the conical flask to fully disperse the oral care product into a homogenous solution (25%) for later use.

[0082] (2) Oral pathogenic bacteria (Streptococcus mutans - ATCC 700610) were cultured normally for 24 h and then washed with PBS to prepare a bacterial suspension (the required concentration was: 100 μL was dropped into 5 mL of PBS control solution, and the number of recovered bacteria was 1×10 4 CFU / mL~9×10 4 CFU / mL).

[0083] (3) Take 3 tubes of each sample solution and blank control group (5 mL) respectively, take the above bacterial suspension, add 100 μL to each sample solution, mix evenly and start timing. After 2 min, mix thoroughly and make appropriate dilutions. Then take 1 mL of each of 2 to 3 dilutions and place them on 2 plates. Pour 15 mL of BHI medium (brain heart infusion agar medium) cooled to 40℃ to 45℃, rotate the plate to make it fully uniform. After the agar solidifies, turn the plate over and incubate at 35℃±2℃ for 48 h to count the number of live bacteria.

[0084] (4) The antibacterial rate was calculated according to the following formula, and the results are summarized in Table 3:

[0085]

[0086] Where: X is the inhibition rate, %; NC is the average colony count of the control sample, CFU / mL; NS is the average colony count of the sample, CFU / mL.

[0087] (5) Evaluation criteria

[0088] If the antibacterial rate is ≥50%~90%, the product has antibacterial effect; if the antibacterial rate is ≥90%, the product has a strong antibacterial effect.

[0089] Table 3

[0090]

[0091] 3. The test steps for plaque staining performance are as follows:

[0092] 3.1 The smears of Examples 1 to 4 and Comparative Examples 1 to 6 were respectively applied to the teeth of volunteers with cotton swabs to observe the tooth staining effect. Ten volunteers were set up in each group (the plaque index of all 100 volunteers was detected using a commercially available plaque display liquid, and the volunteers were divided into 10 groups to ensure that there was no statistical difference between the initial average plaque index of each group of volunteers). The plaque was then stained with the plaque display smear of this embodiment. After staining with the smear in the morning, the staining effect was observed by indicators, and correct brushing was guided according to the staining effect. After brushing, the same commercially available plaque display liquid was used for staining, the plaque on the teeth was checked, and the indicators were observed again according to the same method.

[0093] 3.2 Observation of indicators: Use plaque display liquid to stain the plaque. When the plaque display agent spreads to the tooth surface, apply it to the cheek and tongue surfaces of all teeth, then rinse your mouth with clean water and then observe. The colored area is the area where plaque exists.

[0094] The criteria for judging the results are as follows: 0: no plaque, i.e., the stain is completely eliminated; 1: there is some staining on the tooth surface near the gingival margin, with scattered spot-like plaque; 2: there is a little staining on the tooth surface near the gingival margin, and the thin plaque on the tooth surface is continuous and band-like; 3: the plaque staining band is wider than 1 mm, but the staining coverage area is less than 1 / 3 of the tooth surface; 4: the plaque covers more than 1 / 3 of the tooth surface, but not more than 2 / 3 of the tooth surface, and most of the tooth surface is stained; 5: the plaque covers more than 2 / 3 of the tooth surface, and the stain exists for more than half. The plaque staining is combined with parameters such as area and thickness (staining depth) to comprehensively determine the level of the plaque index, and the results are statistically summarized in Table 4.

[0095] Table 4

[0096] sample Initial plaque index Plaque index after brushing Plaque index change value Example 1 2.5 1.3 1.2 Example 2 2.7 1.2 1.5 Example 3 3.1 1.0 2.1 Example 4 2.5 1.1 1.4 Comparative Example 1 2.0 1.5 0.5 Comparative Example 2 2.8 1.4 1.4 Comparative Example 3 2.0 1.4 0.6 Comparative Example 4 2.5 1.4 1.1 Comparative Example 5 2.5 1.6 0.9 Comparative Example 6 0 2.5 -2.5

[0097] The larger the initial plaque index is, the better the staining effect is (clearer the staining is); the larger the change in the plaque index is, the better the sterilization effect through staining and guiding the brushing method, as well as the antibacterial effect of other active ingredients during staining. That is, the change in the plaque index is determined by the effects of these two aspects and is mainly determined by the sterilization effect of the brushing method.

[0098] It should be noted that in Table 4, after the trial participants used the ointments of Examples 1 to 4, except for Example 3 which had a high content of dye and was too dark to affect the user experience, the initial plaque index of the other examples was large and the change value of the plaque index after brushing was also large (the change value was not less than 1.1), indicating that the use of the ointment of the present invention can effectively dye dental plaque and thus guide mechanical brushing to remove dental plaque, and can also use the other active ingredients to effectively inhibit the growth of dental plaque while dyeing. Compared with Example 1, Comparative Examples 1 and 3 cannot be dyed in a targeted manner and release the remaining active ingredients due to the absence of glucan, resulting in a small initial plaque index and a small change in the plaque index after brushing, indicating that Comparative Examples 1 and 3 cannot effectively guide mechanical removal of dental plaque and that the effect of using the remaining active ingredients to inhibit the growth of dental plaque during dyeing is also poor; Compared with Example 1, Comparative Examples 2 and 4 lack breast milk oligosaccharides, and Comparative Example 5 lacks plaque-decomposing enzymes, so the initial plaque index is equivalent to that of Example 1, but the change in the plaque index after brushing is small, indicating that dyeing the dental plaque and then guiding mechanical brushing to remove the dental plaque while using the remaining active ingredients to inhibit the growth of dental plaque is poor; Compared with Example 1, Comparative Example 6 has no dyeing effect initially due to the absence of a dye, and a large plaque index appears after brushing, indicating that the lack of a dye cannot effectively guide mechanical methods such as brushing to remove plaque.

[0099] Referring to Tables 1 to 4, it can be seen from the test results of Examples 1 to 4 and Comparative Examples 1 and 3 that when the composition for inhibiting dental plaque contains glucan, it helps to stably suspend the active ingredients therein, thereby having relatively excellent stability, so that the oral care product prepared by the composition has relatively excellent stability; at the same time, since the addition of glucan has the function of being able to release active ingredients to dental plaque in a directed and continuous manner, the composition has relatively excellent antibacterial and dyeing effects, and thus the oral care product prepared by the composition has relatively excellent antibacterial and dyeing effects (good dyeing effect corresponds to better mechanical sterilization effect).

[0100] It can be seen from the test results of Example 1 and Comparative Examples 2 and 3 that when the total amount is 0.7%, when the composition for inhibiting dental plaque contains both glucan and human milk oligosaccharides, compared with only one of them, since glucan can assist human milk oligosaccharides to better exert the effect of prebiotics while exerting the prebiotic effect, that is, the prebiotic component can exert the effect of 1+1>2, the former has a better antibacterial effect, and thus the oral care product prepared by the composition has a better antibacterial effect.

[0101] It can be seen from the test results of Example 1 and Comparative Example 5 that when the composition for inhibiting dental plaque contains plaque-decomposing enzymes, the former has a better antibacterial effect than when there is no plaque-decomposing enzymes, thereby making the oral care product prepared by the composition have a better antibacterial effect.

[0102] It can be seen from the test results of Example 1 and Comparative Example 6 that when the composition for inhibiting dental plaque contains a dye, compared with the absence of a dye, the former can better dye the dental plaque, thereby guiding the mechanical method to more thoroughly remove the dental plaque, and thus the oral care product prepared by the composition can better guide the mechanical method to remove dental plaque.

[0103] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present application.

Claims

1. A composition for inhibiting dental plaque, characterized in that: Calculated by mass percentage, it includes: 0.05-0.5% of dextran, 0.1-1% of human milk oligosaccharides, 0.05-0.5% of plaque decomposing enzyme, 0.5-2% of colorant, 0.2-5% of stabilizer, 20-40% of dispersant, 0.1-2% of flavoring agent and the balance of water.

2. The composition for inhibiting dental plaque according to claim 1, characterized in that: The glucan includes β-glucan.

3. The composition for inhibiting dental plaque according to claim 2, characterized in that: The β-glucan is produced by fermentation of Schizophyllum.

4. The composition for inhibiting dental plaque according to any one of claims 1 to 3, characterized in that: The human milk oligosaccharide is selected from at least one of 2'-fucosyllactose, 3-fucosyllactose, lactose-N-neotetraose, 6'-sialyllactose sodium salt and 3'-sialyllactose sodium salt.

5. The composition for inhibiting dental plaque according to any one of claims 1 to 3, characterized in that: The plaque decomposing enzyme includes lysozyme, and the enzyme activity of the lysozyme is not less than 3000 U / mg.

6. The composition for inhibiting dental plaque according to any one of claims 1 to 3, characterized in that: The dye is selected from at least one of basic fuchsin, besmer brown, neutral red, iodine dyes and erythrosine.

7. The composition for inhibiting dental plaque according to any one of claims 1 to 3, characterized in that: The stabilizer is selected from at least one of hydrogenated castor oil, poloxamer, Tween and alkylene oxide-polysiloxane polymer.

8. The composition for inhibiting dental plaque according to any one of claims 1 to 3, characterized in that: The dispersant is selected from at least one of sorbitol, glycerol, polyethylene glycol, propylene glycol, 1,3-butylene glycol, hexylene glycol and ethanol; And / or, the flavoring agent is selected from at least one of a sweetener and an essence.

9. Use of the composition for inhibiting dental plaque according to any one of claims 1 to 8 in the preparation of oral care products.

10. The use according to claim 9, characterized in that: The dosage form of the oral care product is selected from any one of paste, spray, gel and solution.

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