A composition with skin repair effect, use and serum

Abstract

The application belongs to the technical field of skin repair, and discloses a composition with skin repair effect, application and essence. The composition with skin repair effect comprises 20-30 parts of aloe vera leaf water, 8-12 parts of apple fruit cell culture extract, 2-5 parts of grape flower cell extract and 0.5-1 part of Tokyo cherry leaf extract in terms of weight fraction. The aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry leaf extract are compounded, the apple fruit cell culture further promotes the supplement of dermal cells such as fibroblasts under the material supply of the grape flower cell extract and Tokyo cherry leaf extract, the aloe vera leaf water and Tokyo cherry leaf extract promote the formation of the cuticle and the repair of damaged skin in the process, and the skin is repaired in multiple directions, thereby producing the effect of synergistically improving the skin repair effect.

Description

Technical Field

[0001] This invention relates to the field of skin repair technology, specifically to a composition, uses, and serum with skin repair function. Background Technology

[0002] For the current skincare market, products related to skin repair are of paramount importance, primarily due to the following core reasons:

[0003] (1) Damaged skin barrier: The skin barrier is the first line of defense for the skin. It is composed of the stratum corneum and the sebum film, which can prevent moisture loss and the invasion of foreign substances. When the skin barrier is damaged, it will lead to excessive moisture loss, skin sensitivity and susceptibility to external stimuli, which will in turn cause a series of skin problems such as dryness, tightness and redness.

[0004] (2) Skin aging: As we age, the amount of collagen and elastin in our skin gradually decreases, leading to sagging and wrinkles. Proper skin care can slow down the aging process and maintain the firmness and radiance of the skin.

[0005] (3) External environmental stimuli, such as daily ultraviolet radiation and environmental pollution, can damage the skin, leading to problems such as photoaging, dryness, sensitivity and dullness. Skin repair can help repair these skin problems caused by external environmental stimuli and restore the skin to a healthy state.

[0006] Therefore, skin repair is a key direction for skincare product research and development. Existing technology 1: Chinese patent application 202411624692.7 discloses a soothing and repairing composition, which, by mass percentage, includes 45% to 55% raspberry fruit extract solution, 40% to 50% acerola cherry extract solution, and 5% to 15% oat kernel extract powder. The combination of these three ingredients can enhance the soothing and repairing effect of raspberry fruit. In addition, a cosmetic product containing 0.2 to 1.5 wt% of the above composition is also disclosed.

[0007] The description of prior art 1 states: "The raspberry fruit extract solution contains abundant anthocyanins, polyphenols, flavonoids, vitamin C, vitamin E and other active substances, which can repair the skin barrier function, inhibit skin inflammation, and calm excessive nerve reactions to achieve the effect of soothing sensitive skin." This indicates that prior art 1 focuses on the raspberry fruit extract solution to repair the skin barrier function to achieve skin repair; it mainly improves the problem of dry and sensitive skin through the compounding of components.

[0008] However, focusing solely on skin barrier repair is insufficient for skin repair. While it may provide short-term benefits, it doesn't address the underlying skin repair needs. Over time, this requires frequent use of skin repair products. Therefore, a multi-faceted approach to skin repair research is necessary to avoid a situation where the skin requires constant repair. Summary of the Invention

[0009] One of the objectives of this invention is to provide a composition with skin repair function. By combining aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract, and Tokyo cherry blossom leaf extract, the composition can repair the skin from multiple aspects, such as stratum corneum repair, replenishing fibroblasts, and providing supporting structural substances for fibroblasts, thereby improving the skin repair efficacy of the composition.

[0010] Another objective of this invention is to provide a use of a composition with skin-repairing properties in the preparation of cosmetics, wherein the composition with skin-repairing properties, when used in the preparation of cosmetics, can effectively improve the skin-repairing effect of the cosmetics.

[0011] Meanwhile, the present invention also provides an essence containing the skin-repairing composition of the present invention, which has excellent skin-repairing effects.

[0012] To achieve the above objectives, the present invention provides a composition with skin repair function, comprising, by weight, 20-30 parts of aloe vera leaf water, 8-12 parts of apple fruit cell culture extract, 2-5 parts of grape flower cell extract, and 0.5-1 part of Tokyo cherry blossom leaf extract.

[0013] The acetylated polysaccharides in aloe vera leaf water can enhance the skin's resistance, accelerate the healing of damaged tissues, and provide immediate soothing relief. In addition, it can effectively repair skin epidermal cells damaged by ultraviolet rays and promote the rapid recovery of sunburned skin. The various active ingredients in aloe vera leaf water can accelerate the self-repair process of damaged skin.

[0014] Apple fruit cell culture extract can continuously renew the outermost epidermal layer, making the skin smooth and healthy. Human stem cells have the effect of replenishing dermal cells such as fibroblasts and helping wound healing. Apple fruit cell culture extract can provide important nutrients for human stem cells and improve their activity and health, effectively protecting and maintaining the efficacy of skin stem cells. Therefore, apple fruit cell culture extract can effectively promote the replenishment of dermal cells such as fibroblasts and promote wound healing.

[0015] Grape flower cell extract can increase collagen levels and promote proteoglycan synthesis. When skin is damaged, the dermis quickly produces new skin tissue through fibroblasts. These cells produce two essential substances for the skin's supporting structure: collagen and proteoglycans. Therefore, grape flower cell extract can effectively provide fibroblasts with the necessary supporting substances, thus effectively promoting skin repair.

[0016] Certain components in Tokyo cherry blossom leaf extract, such as cherry blossom acid and cherry blossom alkaloid, can promote the formation of the stratum corneum and repair damaged skin; in addition, Tokyo cherry blossom leaf extract can promote the regeneration of collagen in the skin, thereby achieving the purpose of firming the skin and delaying aging.

[0017] This invention utilizes a combination of aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract, and Tokyo cherry blossom leaf extract to synergistically enhance skin repair. The preliminary assessment suggests that the synergistic mechanism is as follows: apple fruit cell culture, with the aid of grape flower cell extract and Tokyo cherry blossom leaf extract, further promotes the replenishment of dermal cells such as fibroblasts. Furthermore, aloe vera leaf water and Tokyo cherry blossom leaf extract promote stratum corneum formation and repair damaged skin during this process. This multi-directional approach to skin repair results in a synergistic enhancement of skin repair efficacy.

[0018] Preferably, the skin-repairing composition of the present invention further includes a solvent.

[0019] More preferably, the solvent includes glycerol, butanediol, water, and phenoxyethanol.

[0020] Furthermore, the skin-repairing composition also includes lecithin and xanthan gum.

[0021] Preferably, the Tokyo cherry blossom leaf extract is obtained by the following steps:

[0022] Step 1: Add Tokyo cherry blossom leaf powder to an ethanol solution with a mass fraction of 40-60% at a material-to-liquid ratio of 35ml / g, then add 5-8% organic acid by weight of Tokyo cherry blossom leaf powder, and extract at an extraction temperature of 60-70℃ for 35-45 minutes, and separate to obtain the filtrate.

[0023] Step 2: Precipitate the filtrate with ethanol for 12-14 hours to obtain an alcohol precipitate, and then concentrate it to obtain an alcohol-free concentrate;

[0024] Step 3: Add an extractant to the alcohol-free concentrate to extract the extract, which is the Tokyo cherry blossom leaf extract.

[0025] Preferably, step 2 can be performed by those skilled in the art using existing alcohol precipitation processes; more preferably, the specific steps of step 2 are as follows: first, concentrate the filtrate to 20% of its original mass using a single-effect concentrator, then add an equal mass of 90% ethanol solution and let it stand for 12-14 hours, centrifuge, take the supernatant to obtain the alcohol precipitate, transfer the alcohol precipitate to a falling film concentrator, and vacuum concentrate the filtrate to zero ethanol content at a concentration temperature of 50-60℃ and a concentration pressure of -0.07MPa to -0.09MPa to obtain an alcohol-free concentrate.

[0026] Preferably, the organic acid is one of tartaric acid, citric acid, and ascorbic acid.

[0027] Preferably, the extractant is at least one of water, butanediol, glycerol, and methylpropanediol, and the amount of extractant added is 1 to 5 times the weight of the Tokyo cherry blossom leaf powder, and the extraction time is 30 to 50 minutes.

[0028] To further improve the content of chlorella alkaloid in Tokyo cherry blossom leaf extract, this invention discloses an extraction method for Tokyo cherry blossom leaf extract. By adding organic acids to the extraction system, the extraction efficiency of chlorella alkaloid is improved without affecting the extraction content of other components in the Tokyo cherry blossom leaf extract. The Tokyo cherry blossom leaf extract obtained by this extraction method has a more significant synergistic effect when combined with aloe vera leaf water, apple fruit cell culture extract, and grape flower cell extract.

[0029] The present invention also discloses the use of the above-mentioned composition with skin repair function in the preparation of cosmetics.

[0030] The present invention also discloses an essence containing 0.5-20% of the above-mentioned composition with skin repair function.

[0031] Beneficial effects

[0032] Compared with the prior art, the present invention has at least the following advantages:

[0033] (1) Apple fruit cell culture extract can continuously renew the outermost epidermal layer, making the skin smooth and healthy; human stem cells have the function of replenishing dermal cells such as fibroblasts and helping wound healing, while apple fruit cell culture extract can provide important nutrients for human stem cells and improve the activity and health of human stem cells, effectively protecting and maintaining the efficacy of skin stem cells. Therefore, apple fruit cell culture extract can effectively promote the replenishment of dermal cells such as fibroblasts and promote wound healing.

[0034] (2) The acetylated polysaccharide component in Aloe vera leaf water can enhance the skin's resistance, accelerate the healing of damaged tissues, and bring an immediate soothing sensation to the skin. In addition, it can effectively repair skin epidermal cells damaged by ultraviolet rays and promote the rapid recovery of sunburned skin. The various active ingredients in Aloe vera leaf water can accelerate the self-repair process of damaged skin.

[0035] (3) Grape flower cell extract can increase the proportion of collagen and promote the synthesis of proteoglycans. After the skin is damaged, the dermis will produce new skin tissue through fibroblasts in a short period of time. These cells produce two substances that are essential for the skin's supporting structure: collagen and proteoglycans. Therefore, grape flower cell extract can effectively provide fibroblasts with the substances needed for the supporting structure and effectively promote skin repair.

[0036] (4) Some components in Tokyo cherry blossom leaf extract, such as cherry acid and cherry alkaloid, can promote the formation of the stratum corneum and repair damaged skin; and Tokyo cherry blossom leaf extract can promote the regeneration of collagen in the skin, so as to tighten the skin and delay aging.

[0037] (5) This invention combines aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry blossom leaf extract. The apple fruit cell culture further promotes the replenishment of dermal cells such as fibroblasts with the help of the grape flower cell extract and Tokyo cherry blossom leaf extract. In addition, the aloe vera leaf water and Tokyo cherry blossom leaf extract promote the formation of the stratum corneum and repair damaged skin in the process. The multi-directional repair of the skin produces a synergistic effect to enhance the skin repair efficacy. Detailed Implementation

[0038] The present invention will be further described below with reference to embodiments, but this does not constitute any limitation on the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0039] To illustrate the technical content of the present invention in detail, the following description is provided in conjunction with the embodiments.

[0040] Unless otherwise specified, in the following examples and comparative examples, the Aloe vera leaf water was purchased from Yunnan Wanlv Biotechnology Co., Ltd., under the trade name Wanlv Aloe Vera Leaf Water A04-3, with an Aloe vera leaf water content of 100wt%; the apple fruit cell culture extract was purchased from Guangzhou Lingyu Trading Co., Ltd., under the trade name Apple Stem Cells, with the composition: water 88.06wt%, apple fruit cell culture extract 9wt%, lecithin 0.14wt%, glycerol 0.4wt%, phenoxyethanol 1.4wt%, xanthan gum 1wt%; and the grape flower cell extract was purchased from NAOLYS, under the trade name Healthy Perfection (Vitis). The following extracts were used: 1) * **flower (2S-Gly):** Grape flower cell extract 20wt%, glycerin 80wt%; 2) Tokyo cherry blossom leaf extract (purchased from Japan's Ichimaru Natural Beauty Co., Ltd., trade name: Tokyo cherry blossom leaf extract), butylene glycol 49wt%, water 49wt%, Tokyo cherry blossom leaf extract 2wt%; 3) Portulaca oleracea extract (purchased from Lanzhou Waterles Biotechnology Co., Ltd., in powder form); 4) Edelweiss callus extract (purchased from Guangzhou Huayang Biotechnology Co., Ltd., trade name: Edelweiss ferment), butylene glycol 78wt%, Edelweiss callus extract 20wt%, Leuconostoc / radish root ferment filtrate 2wt%; 5) Olive leaf extract (purchased from Guangzhou Huayang Biotechnology Co., Ltd., trade name: Olive leaf ferment), 54.4wt%, Lactobacillus 2wt%, olive leaf extract 43.5wt%; 6) Centella asiatica extract (purchased from Xi'an An'ao Biotechnology Co., Ltd., product number: QAD-5803, in powder form.

[0041] The technical solution of the present invention can be implemented by other commercially available products, and is not limited to the raw materials from the above sources.

[0042] Unless otherwise specified, in the following examples and comparative examples, the parts and percentages refer to parts by weight and weight percentage, respectively.

[0043] The composition was prepared according to the formulation table in Table 1, and the preparation method is as follows:

[0044] Add grape flower cell extract or olive leaf extract, aloe vera leaf water or purslane extract, Tokyo cherry blossom leaf extract or centella asiatica extract, apple fruit cell culture extract or edelweiss callus extract to 80 parts of deionized water and stir until completely dissolved.

[0045] It should be noted that in the following examples and comparative examples, deionized water is used as a solvent. The main purpose is to mix grape flower cell extract or olive leaf extract, aloe vera leaf water or purslane extract, Tokyo cherry blossom leaf extract or centella asiatica extract, apple fruit cell culture extract or edelweiss callus extract, which does not affect the efficacy of the composition.

[0046] It should be noted that the Tokyo cherry blossom leaf extracts in Examples 5 and 6 of Table 1 were obtained using the following steps:

[0047] Step 1: Add Tokyo cherry blossom leaf powder to an ethanol solution with a mass ratio of 60% at a material-to-liquid ratio of 35 ml / g, then add citric acid at a mass ratio of 7% of the Tokyo cherry blossom leaf powder, extract at an extraction temperature of 60℃ for 45 min, and separate to obtain the filtrate.

[0048] Step 2: First, concentrate the filtrate to 20% of its original mass using a single-effect concentrator, then add an equal mass of 90% ethanol solution and let it stand for 12-14 hours. Centrifuge and take the supernatant to obtain the alcohol precipitate. Transfer the alcohol precipitate to a falling film concentrator and vacuum concentrate the filtrate to zero ethanol content at a concentration temperature of 50℃ and a concentration pressure of -0.08MPa to obtain an alcohol-free concentrate.

[0049] Step 3: Add 3 times the weight of Tokyo cherry blossom leaf powder in butylene glycol to the alcohol-free concentrate and extract for 45 minutes to obtain the extract, which is the Tokyo cherry blossom leaf extract.

[0050] Table 1. Composition Formulation Table

[0051]

[0052]

[0053] Efficacy test

[0054] 1. Single-component efficacy test

[0055] Test method:

[0056] Fibroblast scratch assay

[0057] 1.1 Experimental Setup

[0058] Component testing: The sample was diluted with pure water to a volume fraction of 4%, then filtered through a 0.22 μm filter, and the filtrate was collected as the sample mother solution.

[0059] Negative controls: basal culture medium (cell viability assay); incomplete culture medium (scratch assay).

[0060] Positive control: basal culture medium.

[0061] 1.2 Experimental Methods

[0062] Fibroblast scratch assay: Fibroblasts were seeded into 6-well plates and cultured for 24 hours. A streak was then made in the center of each well using a sterile pipette tip to create a scratch. The culture medium was then discarded. Basal culture medium (positive control), incomplete culture medium (negative control), and the corresponding sample group culture medium were added. Cell healing was observed and photographed at 0 hours and 24 hours.

[0063] 1.3 Calculation Formula

[0064]

[0065] 1.4 Data Analysis

[0066] Quantitative analysis of cell scratch images at 0h and 24h was performed using Image-pro plus (IPP) image analysis software.

[0067] The extracts of grape flower cells, olive leaves, aloe vera leaves, purslane, Tokyo cherry blossom leaves used in Examples 1-4, Tokyo cherry blossom leaves used in Examples 5-6, centella asiatica extract, apple fruit cell culture extract, and edelweiss callus tissue extract were tested according to the above test methods, and the results are shown in Table 2.

[0068] Table 2 Results of single-component fibroblast scratch assay

[0069]

[0070] 2. Composition efficacy test

[0071] Test method:

[0072] Fibroblast scratch assay

[0073] 2.1 Experimental Setup

[0074] Composition test: Dilute the sample with pure water to a volume fraction of 4%, then filter it through a 0.22 μm filter, and collect the filtrate as the sample mother solution.

[0075] Negative controls: basal culture medium (cell viability assay); incomplete culture medium (scratch assay).

[0076] Positive control: basal culture medium.

[0077] 2.2 Experimental Methods

[0078] Fibroblast scratch assay: Fibroblasts were seeded into 6-well plates and cultured for 24 hours. A streak was then made in the center of each well using a sterile pipette tip to create a scratch. The culture medium was then discarded. Basal culture medium (positive control), incomplete culture medium (negative control), and the corresponding sample group culture medium were added. Cell healing was observed and photographed at 0 hours and 24 hours.

[0079] 2.3 Calculation Formula

[0080]

[0081] 2.4 Data Analysis

[0082] Quantitative analysis of cell scratch images at 0h and 24h was performed using Image-pro plus (IPP) image analysis software.

[0083] The compositions of Examples 1-4 and Comparative Examples 1-8 were tested according to the above test methods, and the results are shown in Table 3.

[0084] Table 3. Results of fibroblast scratch assays of the compositions in Examples 1-4 and Comparative Examples 1-8

[0085]

[0086] According to the results in Tables 2 and 3:

[0087] According to the data from Examples 1-6, the present invention has excellent skin repair effects by combining aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry blossom leaf extract.

[0088] A comparison of data from Examples 3 and 4 shows that increasing the amount of apple fruit cell culture extract and grape flower cell extract resulted in a greater improvement in skin repair efficacy.

[0089] Meanwhile, based on the data comparison between Example 5 and Example 3, and between Example 6 and Example 4, it can be seen that the Tokyo cherry blossom leaf extract obtained by the specific extraction method of the present invention can significantly enhance the skin repair efficacy of the composition. The reason is that the present invention improves the extraction efficiency of cherry blossom alkaloid by adding organic acid to the extraction system, without affecting the extraction content of other components in the Tokyo cherry blossom leaf extract, so that the extracted Tokyo cherry blossom leaf extract has a higher content of cherry blossom alkaloid. It is speculated that in the technical solution of the present invention, cherry blossom alkaloid plays an important role in the synergistic effect of compounding with aloe vera leaf water, apple fruit cell culture extract, and grape flower cell extract.

[0090] Furthermore, a comparison of the data from Examples 5 and 6 shows that, after using the Tokyo cherry blossom leaf extract obtained through the specific extraction steps of this invention, the difference in the 24-hour scratch healing rate of fibroblasts between Examples 5 and 6 is not as large as the difference between Examples 3 and 4. The difference in between should be attributed to the different Tokyo cherry blossom leaf extracts. This further proves that the Tokyo cherry blossom leaf extract obtained through the specific extraction steps of this invention can further enhance the skin repair efficacy of the combination of Tokyo cherry blossom leaf extract with aloe vera leaf water, apple fruit cell culture extract, and grape flower cell extract.

[0091] A comparison of the data from Example 4 and Comparative Examples 1-4 shows that, even when the total amount of components in the composition is the same, the skin repair efficacy of Comparative Examples 1-4 is significantly weaker than that of Example 4, even when any one of the following components is missing: Tokyo cherry blossom leaf extract, aloe vera leaf water, apple fruit cell culture extract, or grape flower cell extract. Furthermore, the skin repair efficacy of Comparative Examples 1-4 varies significantly depending on the missing component. This indicates that the combination of aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract, and Tokyo cherry blossom leaf extract in this invention produces a synergistic effect that enhances the skin repair efficacy of the composition; while the absence of any one component only results in a simple superposition of component effects.

[0092] According to the data in Table 2 and the data in Table 3 for Example 4 and Comparative Example 5, it can be seen that when Comparative Example 5 used purslane extract, which has a higher 24-hour scratch healing rate of fibroblasts, to replace aloe vera leaf water, the resulting composition showed a significantly lower 24-hour scratch healing rate of fibroblasts compared to Example 4. This further illustrates that the combination of aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract, and Tokyo cherry blossom leaf extract in this invention produces a synergistic effect in enhancing the skin repair efficacy of the composition.

[0093] Similarly, according to the data in Table 2 and the data in Table 3 for Example 4 and Comparative Example 6, it can be seen that Comparative Example 6 used Edelweiss callus extract, which has a higher 24-hour scratch healing rate of fibroblasts, instead of apple fruit cell culture extract. The resulting composition showed a significantly lower 24-hour scratch healing rate of fibroblasts compared to Example 4. This further verifies that the combination of Aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract, and Tokyo cherry blossom leaf extract produced a synergistic effect in enhancing the skin repair efficacy of the composition.

[0094] According to the data in Table 2 and the comparison of the data in Table 3 between Example 4 and Comparative Examples 7 and 8, it can be seen that Comparative Example 7 used olive leaf extract, which has a lower 24-hour scratch healing rate of fibroblasts, to replace grape flower cell extract, and Comparative Example 8 used centella asiatica extract, which has a lower 24-hour scratch healing rate of fibroblasts, to replace Tokyo cherry blossom leaf extract. However, the data in Table 2 shows that the difference in 24-hour scratch healing rate of fibroblasts between olive leaf extract and grape flower cell extract, and between centella asiatica extract and Tokyo cherry blossom leaf extract, is not significant. However, after the composition is formed, the 24-hour scratch healing rate of fibroblasts shown in Comparative Examples 7 and 8 is significantly weaker than that in Example 4. This indicates that the combination of aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract, and Tokyo cherry blossom leaf extract in this invention produces a synergistic effect in enhancing the skin repair efficacy of the composition.

[0095] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. A composition having skin-repairing properties, characterized in that, By weight, it includes 20-30 parts aloe vera leaf water, 8-12 parts apple fruit cell culture extract, 2-5 parts grape flower cell extract, and 0.5-1 part Tokyo cherry blossom leaf extract. The aloe vera leaf water was purchased from Yunnan Wanlv Biotechnology Co., Ltd. The apple fruit cell culture extract was purchased from Guangzhou Lingyu Trading Co., Ltd. The grape flower cell extract was purchased from NAOLYS; The Tokyo cherry blossom leaf extract was obtained using the following steps: Step 1: Add Tokyo cherry blossom leaf powder to an ethanol solution with a mass fraction of 40-60%, then add 5-8% organic acid by weight of Tokyo cherry blossom leaf powder, and extract at an extraction temperature of 60-70℃ for 35-45 minutes, then separate to obtain the filtrate. Step 2: Precipitate the filtrate with ethanol for 12-14 hours to obtain an alcohol precipitate, and then concentrate it to obtain an alcohol-free concentrate; Step 3: Add an extractant to the alcohol-free concentrate to extract the extract, which is the Tokyo cherry blossom leaf extract; Alternatively, the Tokyo cherry blossom leaf extract was sourced from Ichimaru Natural Beauty Co., Ltd. in Japan.

2. The composition with skin-repairing effect according to claim 1, characterized in that, It also includes solvents.

3. Use of a composition having skin-repairing properties as described in any one of claims 1-2 in the preparation of a cosmetic.

4. An essence, characterized in that, Containing 0.5-20% of the skin-repairing composition according to any one of claims 1-2.

Images