Anti-hair loss composition containing Platycladus orientalis leaves, and preparation method and application thereof

Abstract

The present invention provides an anti-hair loss composition containing Platycladus orientalis leaves, and its preparation method and application, belonging to the technical field of biopharmaceuticals. The anti-hair loss composition containing Platycladus orientalis leaves of the present invention comprises: Platycladus orientalis leaf extract, ginseng root extract, snakehead intestine extract, Polygonum multiflorum extract, tea seed extract, Gleditsia extract, mulberry bark extract, Dictamnus root bark extract, Cnidium monnieri seed extract, and peppermint extract. The components of the present invention have a synergistic effect, improving the oily environment of the scalp, preventing oil from clogging hair follicles, reducing damage to hair follicles caused by inflammation, inhibiting 5α-reductase activity, improving hair color and toughness, and thereby alleviating seborrheic alopecia and achieving an anti-hair loss effect.

Description

Technical Field

[0001] The present invention relates to the technical field of biopharmaceuticals, and in particular to an anti-hair loss composition containing Platycladus orientalis leaves, and a preparation method and application thereof. Background Art

[0002] With the accelerating pace of modern life, increasing work pressure, and changing living environments, hair loss is becoming increasingly common, seriously impacting people's quality of life and mental health. According to relevant statistics, the number of people suffering from hair loss is increasing year by year worldwide, reaching hundreds of millions, and the prevalence of hair loss is increasing at a younger age. Hair loss not only damages personal image but can also lead to psychological problems such as low self-esteem and anxiety, negatively impacting patients' social, work, and daily lives.

[0003] Currently, some chemical hair loss prevention products on the market, while effective, carry significant side effects. For example, the common drug finasteride is only suitable for men with androgenic alopecia and is contraindicated in women. It can also cause adverse reactions such as decreased libido and erectile dysfunction. While minoxidil can be used by both men and women, some users experience allergic symptoms such as scalp irritation, itching, and redness. Long-term use can also lead to drug dependence, and upon discontinuation, hair loss can recur and worsen. Physical treatments, such as laser therapy, stimulate hair follicles through low-energy lasers to promote hair growth, but require long-term use, and the equipment is expensive, making the treatment cost prohibitive for most families. Furthermore, frequent laser use can potentially damage scalp tissue and affect the skin's normal physiological functions. Hair transplant surgery offers a more direct solution to hair loss, but it is invasive and carries risks of infection, bleeding, and scarring. Furthermore, hair transplant surgery is expensive and requires a high level of the patient's own hair follicle resources, making it not suitable for all hair loss patients. At the same time, the survival rate of hair follicles after hair transplantation is not 100%, and multiple surgeries may be required, increasing the patient's pain and financial burden.

[0004] Natural plant extracts are gaining increasing attention for their use in anti-hair loss products due to their relative safety and minimal side effects. Many plant extracts contain multiple active ingredients that can act on hair follicles through various pathways, regulating their growth cycle and promoting hair growth. Therefore, the development of a safe, effective, and long-lasting anti-hair loss composition based on natural ingredients is of great practical significance. The present invention aims to provide an anti-hair loss composition containing Platycladus orientalis leaves. By rationally combining multiple active ingredients to achieve a synergistic effect, this composition overcomes the shortcomings of existing anti-hair loss products and provides a superior solution for those suffering from hair loss. Summary of the Invention

[0005] In response to the above technical problems, the object of the present invention is to provide an anti-hair loss composition containing Platycladus orientalis leaves, and a preparation method and application thereof. The anti-hair loss composition provided by the present invention achieves the effect of anti-hair loss by rationally combining multiple active ingredients to exert a synergistic effect.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides an anti-hair loss composition containing Platycladus orientalis leaves. The composition comprises the following components in parts by weight: 15-25 parts of Platycladus orientalis leaf extract, 8-12 parts of ginseng root extract, 10-20 parts of snakehead intestine extract, 10-20 parts of polygonum multiflorum extract, 5-10 parts of tea seed extract, 5-10 parts of sapodilla fruit extract, 5-10 parts of mulberry bark extract, 3-8 parts of difficile root bark extract, 3-8 parts of cnidium monnieri seed extract and 2-5 parts of mint extract.

[0008] Preferably, the Platycladus orientalis leaf extract is obtained by enzymatic hydrolysis with serrapeptase and nattokinase; and the Panax ginseng root extract is obtained by enzymatic hydrolysis with cellulase and snail protease in sequence.

[0009] Preferably, the snakehead intestine extract is obtained by fermenting Bifidobacterium longum and Lactobacillus delbrueckii subsp. bulgaricus; and the Polygonum multiflorum extract is obtained by fermenting Bacillus licheniformis and Clostridium butyricum in sequence.

[0010] Preferably, the tea seed extract, mulberry bark extract, Dictamni root bark extract and Cnidium monnieri seed extract are obtained by extraction with ethanol solution.

[0011] Preferably, the soapberry extract is obtained by water extraction.

[0012] Preferably, the peppermint extract is obtained by distillation.

[0013] The present invention also provides a preparation method of the above-mentioned anti-hair loss composition, characterized in that: the Platycladus orientalis leaf extract, ginseng root extract, Channa intestine extract, Polygonum multiflorum extract, tea seed extract, Gleditsia extract, Morus alba bark extract, Dictamni root bark extract, Cnidium monnieri seed extract and mint extract are mixed in parts by weight, and sterilized to obtain the anti-hair loss composition.

[0014] The present invention also provides the use of the anti-hair loss composition in the preparation of an anti-hair loss product.

[0015] Preferably, the anti-hair loss product is a skin external preparation for topical application.

[0016] Preferably, the anti-hair loss product can inhibit the activity of 5α-reductase and alleviate seborrheic alopecia.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention provides an anti-hair loss composition containing Platycladus orientalis leaves, comprising: Platycladus orientalis leaf extract, ginseng root extract, snakehead intestine extract, Polygonum multiflorum extract, tea seed extract, Gleditsia sinensis extract, mulberry bark extract, Dictamni root bark extract, Cnidium monnieri seed extract, and peppermint extract. The Platycladus orientalis leaf extract of the present invention can regulate the hair follicle cycle, prompting hair follicles to enter the growth phase from the resting phase; the ginseng root extract stimulates the proliferation of dermal papilla cells of hair follicles, enhances cell viability, and regulates the expression of proteins related to the hair follicle cycle. The two work together to regulate the hair follicle cycle from different aspects and jointly promote hair regeneration. The Polygonum multiflorum extract of the present invention is rich in various nutrients and can nourish hair and improve its color and toughness; the snakehead intestine extract also provides nutrients for hair. The two work synergistically to nourish hair in all directions, enhance hair health, and reduce hair loss caused by malnutrition. The surfactants in the tea seed extract and Chinese honey locust extract of the present invention can clean scalp oil and dirt, keeping hair follicles unobstructed. The mulberry bark extract regulates scalp water metabolism, jointly improving the greasy environment of the scalp and preventing oil from clogging hair follicles, creating a favorable foundation for hair growth. The Dictamnus radix bark extract of the present invention has the effects of clearing heat and dampness, dispelling wind and detoxifying, and can alleviate scalp inflammation. The Cnidium monnieri seed extract can improve the cold and dampness of the scalp and also has a certain inhibitory effect on scalp inflammation. They work synergistically to reduce the damage caused by inflammation to hair follicles, creating a healthy scalp microenvironment for normal hair follicle growth. The flavonoids in the Platycladus orientalis leaves, the ginsenosides in ginseng, and the anthraquinones in Polygonum multiflorum of the present invention have synergistic effects in terms of anti-oxidation, promoting blood circulation, and regulating immunity. They can reduce free radical damage to hair follicle cells, maintain the normal function of hair follicle cells, and jointly enhance the improvement effect on hair loss. The various components of the present invention have a synergistic effect, which has the effects of improving the oily environment of the scalp, preventing oil from clogging hair follicles, reducing damage to hair follicles caused by inflammation, inhibiting 5α-reductase activity, improving hair color and toughness, etc., alleviating seborrheic alopecia, and achieving an anti-hair loss effect. DETAILED DESCRIPTION

[0019] The invention provides an anti-hair loss composition containing Platycladus orientalis leaves. The composition comprises the following components in parts by weight: 15-25 parts of Platycladus orientalis leaf extract, 8-12 parts of ginseng root extract, 10-20 parts of snakehead intestine extract, 10-20 parts of polygonum multiflorum extract, 5-10 parts of tea seed extract, 5-10 parts of sapodilla fruit extract, 5-10 parts of mulberry bark extract, 3-8 parts of difficile root bark extract, 3-8 parts of cnidium monnieri seed extract and 2-5 parts of mint extract.

[0020] The Platycladus orientalis leaf extract of the present invention is preferably obtained by enzymatic hydrolysis of serrapeptase and nattokinase: the dried Platycladus orientalis leaves are crushed through a 100-200 mesh sieve, mixed with water at a solid-liquid ratio of 1:8-12 g / ml, 0.3%-0.5% of serrapeptase (enzyme activity of 20,000-50,000 U / g) and 0.1%-0.4% of nattokinase (enzyme activity of 50,000-100,000 U / g) by weight of the Platycladus orientalis leaves are added, and enzymatic hydrolysis is carried out at 40-50° C. and pH 7.5-8 for 1-3 hours, and the filtrate is filtered to obtain the Platycladus orientalis leaf extract. The Platycladus orientalis leaf extract obtained by the extraction method of the present invention improves scalp blood circulation, regulates the physiological function of hair follicles, and promotes hair follicles to enter the growth phase from the resting phase, which plays a key role in promoting hair growth.

[0021] The ginseng root extract of the present invention is preferably obtained by enzymatic hydrolysis with cellulase and snail protease in sequence: dried ginseng root is crushed through a 100-200 mesh sieve, mixed with water at a material-to-liquid ratio of 10-15 g / ml, and 0.4%-0.8% of the weight of the ginseng root cellulase (enzyme activity of 100,000-200,000 U / g) is added. Enzymatic hydrolysis is carried out at 45-55°C and a pH of 4.5-5.5 for 3-5 hours. 0.3%-0.5% of the weight of the ginseng root snail protease (enzyme activity of 400,000-500,000 U / g) is added. Enzymatic hydrolysis is carried out at 33-37°C and a pH of 5.5-7 for 1-3 hours. The filtrate obtained is the ginseng root extract. The ginseng root extract obtained by the extraction method of the present invention can provide sufficient nutrition to the scalp and hair follicles, promote cell metabolism, enhance hair follicle vitality, better promote hair growth, and enhance hair germination.

[0022] The snakehead intestine extract of the present invention is preferably obtained by fermentation with Bifidobacterium longum and Lactobacillus delbrueckii subspecies bulgaricus: the dried snakehead intestine is crushed through an 80-100 mesh sieve, mixed with water at a material-liquid ratio of 1:12-15g / ml, inoculated with 1%-3% of the weight of the snakehead intestine Bifidobacterium longum (CCTCC NO: M 2019028, with a bacterial activity of 1.3 billion-1.8 billion CFU / g) and 0.8%-1.5% of the weight of the snakehead intestine Lactobacillus delbrueckii subspecies bulgaricus (CCTCC NO: M 2019203, with a bacterial activity of 2 billion-3 billion CFU / g), fermented at 33-38°C and pH 6.2-7 for 2-4 days, filtered with a 0.22-0.45μm filter membrane, and sterilized to obtain the filtrate as the snakehead intestine extract. The snakehead intestine extract obtained by the extraction method of the present invention nourishes the liver and kidneys, replenishes essence and blood, and improves hair loss.

[0023] The extract of Polygonum multiflorum of the present invention is preferably obtained by fermenting Bacillus licheniformis and Clostridium butyricum in sequence: the dried Polygonum multiflorum is crushed through an 80-100 mesh sieve, mixed with water at a material-liquid ratio of 1:15-20 g / ml, and inoculated with 0.5%-1.5% of the weight of Polygonum multiflorum with Bacillus licheniformis. 10103, bacterial activity of 2 billion to 4 billion CFU / g), after aerobic fermentation at 35-40 ° C, pH 6.5-7 for 1-3 days, inoculate 1% to 3% of the weight of Polygonum multiflorum with Clostridium butyricum 24854, with a bacterial activity of 300 million to 800 million CFU / g) is fermented at 33-38°C and a pH of 5.5-6.5 for 2-4 days, filtered through a 0.22-0.45 μm filter membrane, and sterilized. The resulting filtrate is the Polygonum multiflorum extract. The Polygonum multiflorum extract obtained using the extraction method of the present invention contains various nutrients that can nourish hair, improve hair color and toughness, and reduce hair loss caused by malnutrition.

[0024] The tea seed extract of the present invention is preferably obtained by ethanol extraction: dried tea seeds are crushed through a 100-200 mesh sieve, mixed with a 55%-65% ethanol solution at a material-to-liquid ratio of 1:7-13 g / ml, extracted at 45-55°C and 150-250 W ultrasonic power for 30-60 minutes, and filtered. The filtrate is the tea seed extract. The tea seed extract obtained by the extraction method of the present invention can remove scalp oil and dirt, keep hair follicles patency, and reduce the adverse effects of excessive scalp oil on hair follicles.

[0025] The mulberry bark extract of the present invention is preferably obtained by ethanol extraction: dried mulberry bark is pulverized through a 100-200 mesh sieve, mixed with a 45%-55% ethanol solution at a material-to-liquid ratio of 1:5-10 g / ml, extracted at 55-65°C and 250-350 W ultrasonic power for 20-30 minutes, and filtered. The filtrate is the mulberry bark extract. The mulberry bark extract obtained by the extraction method of the present invention can regulate scalp water metabolism and improve the scalp environment.

[0026] The Dictamni root bark extract of the present invention is preferably obtained by ethanol extraction: dried Dictamni root bark is crushed and passed through a 100-200 mesh sieve, mixed with a 70%-80% ethanol solution at a solid-liquid ratio of 1:8-12 g / ml, extracted at 65-75° C. and 200-300 W ultrasonic power for 25-35 minutes, and filtered. The filtrate is the Dictamni root bark extract. The Dictamni root bark extract obtained by the extraction method of the present invention can prevent scalp follicle inflammation caused by dampness, heat toxins, etc., and prevent inflammation from damaging hair follicles and causing hair loss.

[0027] The Cnidium monnieri seed extract of the present invention is preferably obtained by ethanol extraction: dried Cnidium monnieri seeds are crushed through a 100-200 mesh sieve, mixed with a 60%-70% ethanol solution at a material-to-liquid ratio of 1:6-10 g / ml, extracted at 60-70°C and 150-250 W ultrasonic power for 20-40 minutes, and filtered. The filtrate is the Cnidium monnieri seed extract. The Cnidium monnieri seed extract obtained using the extraction method of the present invention can improve cold and dampness in the scalp, promote blood circulation in the scalp, provide sufficient nutrition to hair follicles, and promote the repair and regeneration of hair follicle cells.

[0028] The honey locust extract of the present invention is preferably obtained by water extraction: dried honey locust is crushed through an 80-100 mesh sieve, mixed with water at a solid-liquid ratio of 13-17 g / ml, extracted with stirring at 85-95°C for 10-20 minutes, and filtered. The filtrate is the honey locust extract. The honey locust extract obtained by the extraction method of the present invention can remove oil and dirt from the scalp, inhibit microbial growth, reduce the incidence of scalp inflammation, reduce oxidative stress damage to hair follicle cells, protect the normal function of hair follicle cells, and enhance hair strength and toughness, reducing hair breakage and shedding.

[0029] The mint extract of the present invention is preferably obtained by distillation: dried mint is crushed through a 20-60 mesh sieve, mixed with water at a material-to-liquid ratio of 1:6-10 g / ml, soaked for 2-4 hours, and then heated to 102-110°C for 3-5 hours for distillation extraction. The distillate is collected as the mint extract. The mint extract obtained by the extraction method of the present invention can guide the efficacy of other medications to the head, promoting their delivery to hair follicles, better targeting the scalp and hair follicles, and enhancing the anti-hair loss effect.

[0030] The present invention also provides a preparation method of the above-mentioned anti-hair loss composition, characterized in that: the Platycladus orientalis leaf extract, ginseng root extract, Channa intestine extract, Polygonum multiflorum extract, tea seed extract, Gleditsia extract, Morus alba bark extract, Dictamni root bark extract, Cnidium monnieri seed extract and mint extract are mixed in parts by weight, and sterilized to obtain the anti-hair loss composition.

[0031] The present invention also provides the use of the anti-hair loss composition in the preparation of an anti-hair loss product.

[0032] The hair loss prevention product of the present invention is a topical preparation for external use on the skin. The product comprises the aforementioned hair loss prevention composition and an additive or carrier acceptable in cosmetics or pharmaceuticals, wherein the additive or carrier may be a preservative, antioxidant, emulsifier, moisturizer, lubricant, thickener, penetration enhancer, surfactant, and excipient.

[0033] The anti-hair loss product of the present invention can inhibit the activity of 5α-reductase and alleviate seborrheic alopecia.

[0034] The method of using the anti-hair loss composition of the present invention is as follows: after washing and drying the hair, dilute the anti-hair loss composition with water 4-6 times, spray it evenly on the hair loss area of ​​the scalp in the morning and evening, and after application, gently massage the scalp with the fingertips for 5 minutes.

[0035] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] In the following examples, unless otherwise specified, all methods are conventional.

[0037] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0038] Example 1

[0039] Preparation of anti-hair loss composition containing Platycladus orientalis leaves

[0040] (1) Preparation of each extract

[0041] Platycladus orientalis leaf extract: The dried Platycladus orientalis leaves were crushed through a 150-mesh sieve, mixed with water at a solid-liquid ratio of 1:10 g / ml, 0.4% of serrapeptase (enzyme activity of 30,000 U / g) by weight of the Platycladus orientalis leaves and 0.3% of nattokinase (enzyme activity of 80,000 U / g) by weight of the Platycladus orientalis leaves were added, and enzymatic hydrolysis was carried out at 45°C and pH 7.8 for 2 hours. The enzyme was inactivated by heating to 85°C for 15 minutes, and the filtrate was filtered to obtain the Platycladus orientalis leaf extract;

[0042] Ginseng root extract: Dried ginseng root was crushed through a 150-mesh sieve, mixed with water at a solid-liquid ratio of 1:13 g / ml, and 0.6% of the weight of the ginseng root cellulase (enzyme activity of 150,000 U / g) was added. Enzyme hydrolysis was carried out at 50°C and pH 5 for 4 hours. Snail protease (enzyme activity of 450,000 U / g) was added at 0.4% of the weight of the ginseng root and enzymatic hydrolysis was carried out at 35°C and pH 6 for 3 hours. The enzyme was inactivated by heating to 85°C for 15 minutes and filtered. The filtrate obtained was the ginseng root extract;

[0043] Snake intestine extract: The dried snakehead intestine was crushed and passed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:14 g / ml, and inoculated with 2% of the weight of the snakehead intestine Bifidobacterium longum (CCTCC NO: M 2019028, with a bacterial activity of 1.5 billion CFU / g) and 1% of the weight of the snakehead intestine Lactobacillus delbrueckii subsp. bulgaricus (CCTCC NO: M 2019203, with a bacterial activity of 2.5 billion CFU / g). The mixture was fermented at 37°C and pH 6.5 for 3 days. The fermentation broth was filtered through a 0.22 μm filter membrane and sterilized to obtain the filtrate as the snakehead intestine extract;

[0044] Polygonum multiflorum extract: The dried Polygonum multiflorum was crushed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:17 g / ml, and inoculated with 1% of the weight of Polygonum multiflorum with Bacillus licheniformis. 10103, bacterial activity of 3 billion CFU / g), after aerobic fermentation at 37 ° C, pH 6.7 for 2 days, inoculated with Clostridium butyricum (2% of the weight of Polygonum multiflorum) 24854, with a bacterial activity of 500 million CFU / g) was anaerobically fermented at 37° C. and pH 6 for 3 days, and the fermentation broth was filtered through a 0.22 μm filter membrane and sterilized to obtain the filtrate as the Polygonum multiflorum extract.

[0045] Tea seed extract: dried tea seeds were crushed through a 150-mesh sieve, mixed with a 60% by volume ethanol solution at a solid-liquid ratio of 1:10 g / ml, extracted at 50°C and 200 W ultrasonic power for 50 minutes, and filtered. The filtrate was the tea seed extract;

[0046] Mulberry bark extract: dried mulberry bark was crushed through a 150-mesh sieve, mixed with a 50% by volume ethanol solution at a solid-liquid ratio of 1:8 g / ml, extracted at 60°C and 300W ultrasonic power for 25 minutes, and filtered. The filtrate was the mulberry bark extract;

[0047] Dictamni root bark extract: The dried Dictamni root bark was crushed through a 150-mesh sieve, mixed with a 75% by volume ethanol solution at a solid-liquid ratio of 1:10 g / ml, extracted at 70°C and 250 W ultrasonic power for 30 min, and filtered to obtain the Dictamni root bark extract;

[0048] Cnidium monnieri seed extract: dried Cnidium monnieri seeds were crushed through a 150-mesh sieve, mixed with a 65% by volume ethanol solution at a solid-liquid ratio of 1:8 g / ml, extracted at 65°C and 200W ultrasonic power for 30 minutes, and filtered. The filtrate was the Cnidium monnieri seed extract;

[0049] Gleditsia sinensis extract: Grind the dried Gleditsia sinensis through a 90-mesh sieve, mix with water at a solid-liquid ratio of 1:15 g / ml, stir and extract at 90°C and 60 rpm for 15 min, filter, and the filtrate is the Gleditsia sinensis extract;

[0050] Peppermint extract: dried peppermint was crushed through a 50-mesh sieve, mixed with water at a solid-liquid ratio of 1:8 g / ml, soaked for 3 hours, and then distilled and extracted at 105°C for 4 hours. The distillate was collected as the peppermint extract;

[0051] (2) Preparation of anti-hair loss composition

[0052] The following weight portions were weighed: 20 parts by weight of Platycladus orientalis leaf extract, 10 parts by weight of Panax ginseng root extract, 15 parts by weight of Echinops intestinal extract, 15 parts by weight of Polygonum multiflorum extract, 8 parts by weight of Camellia oleifera seed extract, 8 parts by weight of Gleditsia sinensis extract, 8 parts by weight of Morus alba bark extract, 5 parts by weight of Dictamnus dasyphylla root bark extract, 5 parts by weight of Cnidium monnieri seed extract and 3 parts by weight of peppermint extract. The components were mixed and stirred at 200 r / min for 10 minutes and sterilized to obtain an anti-hair loss composition.

[0053] Example 2

[0054] Preparation of anti-hair loss composition containing Platycladus orientalis leaves

[0055] (1) Preparation of each extract

[0056] Platycladus orientalis leaf extract: The dried Platycladus orientalis leaves were crushed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:8 g / ml, 0.3% of serrapeptase (enzyme activity of 20,000 U / g) by weight of the Platycladus orientalis leaves and 0.1% of nattokinase (enzyme activity of 50,000 U / g) by weight of the Platycladus orientalis leaves were added, and enzymatic hydrolysis was carried out at 40°C and pH 7.5 for 3 hours. The enzyme was inactivated by heating to 90°C for 10 minutes, and the filtrate was filtered to obtain the Platycladus orientalis leaf extract;

[0057] Ginseng root extract: Grind dried ginseng root through a 100-mesh sieve, mix with water at a solid-liquid ratio of 1:10 g / ml, add 0.4% cellulase (enzyme activity of 100,000 U / g) by weight of the ginseng root, and enzymatically hydrolyze at 45°C and pH 4.5 for 5 hours. Add 0.3% snail protease (enzyme activity of 400,000 U / g) by weight of the ginseng root, and enzymatically hydrolyze at 33°C and pH 5.5 for 3 hours. Heat to 90°C to inactivate the enzyme for 10 minutes, and filter to obtain the filtrate as the ginseng root extract;

[0058] Snake intestine extract: The dried snakehead intestine was crushed and passed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:12 g / ml, and inoculated with 1% of the weight of the snakehead intestine Bifidobacterium longum (CCTCC NO: M 2019028, with a bacterial activity of 1.3 billion CFU / g) and 0.8% of the weight of the snakehead intestine Lactobacillus delbrueckii subsp. bulgaricus (CCTCC NO: M 2019203, with a bacterial activity of 2 billion CFU / g). The mixture was fermented at 33°C and pH 6.2 for 4 days. The fermentation broth was filtered through a 0.22 μm filter membrane and sterilized to obtain the filtrate as the snakehead intestine extract;

[0059] Polygonum multiflorum extract: The dried Polygonum multiflorum was crushed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:15 g / ml, and inoculated with 0.5% of the weight of Polygonum multiflorum with Bacillus licheniformis. 10103, bacterial activity of 2 billion CFU / g), after aerobic fermentation at 35 ° C, pH 6.5 for 3 days, inoculated with 1% of the weight of Polygonum multiflorum Clostridium butyricum 24854, bacterial activity of 300 million CFU / g) was fermented at 33°C and pH 5.5 for 4 days, and the fermentation broth was filtered through a 0.22 μm filter membrane and sterilized to obtain the filtrate as the Polygonum multiflorum extract;

[0060] Tea seed extract: dried tea seeds were crushed through a 100-mesh sieve, mixed with 55% ethanol solution by volume at a solid-liquid ratio of 1:7 g / ml, extracted at 45°C and 150W ultrasonic power for 60 minutes, and filtered. The filtrate was the tea seed extract;

[0061] Mulberry bark extract: dried mulberry bark was crushed through a 100-mesh sieve, mixed with a 45% by volume ethanol solution at a solid-liquid ratio of 1:5 g / ml, extracted at 55°C and 250W ultrasonic power for 30 minutes, and filtered. The filtrate was the mulberry bark extract;

[0062] Dictamni root bark extract: The dried Dictamni root bark was crushed through a 100-mesh sieve, mixed with 70% ethanol solution by volume at a solid-liquid ratio of 1:8 g / ml, extracted at 65°C and 200 W ultrasonic power for 35 min, and filtered to obtain the Dictamni root bark extract;

[0063] Cnidium monnieri seed extract: Crush the dried Cnidium monnieri seeds through a 100-mesh sieve, mix with a 60% by volume ethanol solution at a solid-liquid ratio of 1:6 g / ml, extract at 60°C and 150W ultrasonic power for 40 minutes, filter, and the filtrate is the Cnidium monnieri seed extract;

[0064] Gleditsia sinensis extract: Grind the dried Gleditsia sinensis through an 80-mesh sieve, mix with water at a solid-liquid ratio of 1:13 g / ml, stir at 70 rpm for 20 min at 85°C, and filter. The filtrate is the Gleditsia sinensis extract;

[0065] Peppermint extract: dried peppermint was crushed through a 20-mesh sieve, mixed with water at a solid-liquid ratio of 1:6 g / ml, soaked for 4 hours, and then heated and distilled at 102° C. for 5 hours. The distillate was collected as the peppermint extract;

[0066] (2) Preparation of anti-hair loss composition

[0067] The following weight portions were weighed: 15 parts by weight of Platycladus orientalis leaf extract, 8 parts by weight of Panax ginseng root extract, 10 parts by weight of Echinops intestinalis extract, 10 parts by weight of Polygonum multiflorum extract, 5 parts by weight of Camellia oleifera seed extract, 5 parts by weight of Gleditsia sinensis extract, 5 parts by weight of Morus alba bark extract, 3 parts by weight of Dictamnus dasyphylla root bark extract, 3 parts by weight of Cnidium monnieri seed extract and 2 parts by weight of peppermint extract. The components were mixed and stirred at 150 r / min for 15 minutes and sterilized to obtain an anti-hair loss composition.

[0068] Example 3

[0069] Preparation of anti-hair loss composition containing Platycladus orientalis leaves

[0070] (1) Preparation of each extract

[0071] Platycladus orientalis leaf extract: The dried Platycladus orientalis leaves were crushed through a 200-mesh sieve, mixed with water at a solid-liquid ratio of 1:12 g / ml, 0.5% of serrapeptase (enzyme activity of 50,000 U / g) by weight of the Platycladus orientalis leaves and 0.4% of nattokinase (enzyme activity of 100,000 U / g) by weight of the Platycladus orientalis leaves were added, and enzymatic hydrolysis was carried out at 50°C and pH 8 for 1 hour. The enzyme was inactivated at 80°C for 20 minutes, and the filtrate was filtered to obtain the Platycladus orientalis leaf extract;

[0072] Ginseng root extract: Grind dried ginseng root through a 200-mesh sieve, mix with water at a solid-liquid ratio of 1:15 g / ml, add 0.8% cellulase (enzyme activity of 200,000 U / g) by weight of the ginseng root, and enzymatically hydrolyze at 55°C and pH 5.5 for 3 hours. Add 0.5% snail protease (enzyme activity of 500,000 U / g) by weight of the ginseng root, and enzymatically hydrolyze at 37°C and pH 7 for 1 hour. Heat to 80°C to inactivate the enzyme for 20 minutes, and filter to obtain the filtrate as the ginseng root extract;

[0073] Snake intestine extract: The dried snakehead intestine was crushed and passed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:15 g / ml, and inoculated with 3% of the weight of the snakehead intestine Bifidobacterium longum (CCTCC NO: M 2019028, with a bacterial activity of 1.8 billion CFU / g) and 1.5% of the weight of the snakehead intestine Lactobacillus delbrueckii subsp. bulgaricus (CCTCC NO: M 2019203, with a bacterial activity of 3 billion CFU / g). The mixture was fermented at 38°C and pH 7 for 2 days. The fermentation broth was filtered through a 0.45 μm filter membrane and sterilized to obtain the filtrate as the snakehead intestine extract;

[0074] Polygonum multiflorum extract: The dried Polygonum multiflorum was crushed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:20 g / ml, and inoculated with 1.5% of the weight of Polygonum multiflorum with Bacillus licheniformis. 10103, bacterial activity of 4 billion CFU / g), after aerobic fermentation at 40 ° C, pH 7 for 1 day, inoculated with 3% of the weight of Polygonum multiflorum Clostridium butyricum 24854, with a bacterial activity of 800 million CFU / g) was anaerobically fermented at 38°C and pH 6.5 for 2 days, and the fermentation broth was filtered through a 0.45 μm filter membrane and sterilized to obtain the filtrate as the Polygonum multiflorum extract;

[0075] Tea seed extract: dried tea seeds were crushed through a 200-mesh sieve, mixed with a 65% by volume ethanol solution at a solid-liquid ratio of 1:13 g / ml, extracted at 55°C and 250W ultrasonic power for 30 minutes, and filtered. The filtrate was the tea seed extract;

[0076] Mulberry bark extract: dried mulberry bark was crushed through a 200-mesh sieve, mixed with a 55% by volume ethanol solution at a solid-liquid ratio of 1:10 g / ml, extracted at 65°C and 350W ultrasonic power for 20 minutes, and filtered. The filtrate was the mulberry bark extract;

[0077] Dictamni root bark extract: The dried Dictamni root bark was crushed through a 200-mesh sieve, mixed with 80% ethanol solution by volume at a solid-liquid ratio of 1:12 g / ml, extracted at 75°C and 300 W ultrasonic power for 25 min, and filtered to obtain the Dictamni root bark extract;

[0078] Cnidium monnieri seed extract: dried Cnidium monnieri seeds were crushed through a 200-mesh sieve, mixed with 70% ethanol solution by volume at a solid-liquid ratio of 1:10 g / ml, extracted at 70°C and 250W ultrasonic power for 20 minutes, and filtered. The filtrate was the Cnidium monnieri seed extract;

[0079] Gleditsia sinensis extract: Grind the dried Gleditsia sinensis through a 100-mesh sieve, mix with water at a solid-liquid ratio of 1:17 g / ml, extract at 95°C for 10 min, filter, and the filtrate is the Gleditsia sinensis extract;

[0080] Peppermint extract: dried peppermint was crushed through a 60-mesh sieve, mixed with water at a solid-liquid ratio of 1:10 g / ml, soaked for 4 hours, and then distilled and extracted at 110° C. for 3 hours. The distillate was collected as the peppermint extract;

[0081] (2) Preparation of anti-hair loss composition

[0082] The following weight portions were weighed: 25 parts by weight of Platycladus orientalis leaf extract, 12 parts by weight of Panax ginseng root extract, 20 parts by weight of Echinops intestinalis extract, 20 parts by weight of Polygonum multiflorum extract, 10 parts by weight of Camellia oleifera seed extract, 10 parts by weight of Gleditsia sinensis extract, 10 parts by weight of Morus alba bark extract, 8 parts by weight of Dictamnus dasyphylla root bark extract, 8 parts by weight of Cnidium monnieri seed extract and 5 parts by weight of peppermint extract. The components were mixed and stirred at 250 r / min for 8 minutes and sterilized to obtain an anti-hair loss composition.

[0083] Comparative Example 1

[0084] The specific implementation method is the same as that of Example 1, except that when preparing the Platycladus orientalis leaf extract, serrapeptase and nattokinase are replaced by trypsin with an enzyme activity of 30,000 U / g and bromelain with an enzyme activity of 80,000 U / g, and enzymatic hydrolysis is carried out at 40° C. and pH 7.8 for 2 h.

[0085] Comparative Example 2

[0086] The specific implementation method is the same as that of Example 1, except that the enzymatic hydrolysis order of cellulase and snail protease is swapped when preparing the ginseng root extract.

[0087] Comparative Example 3

[0088] The specific embodiment is the same as Example 1, except that when preparing the snakehead intestine extract, Bifidobacterium longum is replaced by Bifidobacterium longum BB536 with a bacterial activity of 1.5 billion CFU / g, and the inoculation amount is 2% of the weight of the snakehead intestine; Lactobacillus delbrueckii subspecies bulgaricus is replaced by Lactobacillus helveticus with a bacterial activity of 2.5 billion CFU / g. 6024), the inoculum size was 1% of the weight of snakehead intestine, and the fermentation conditions remained unchanged.

[0089] Comparative Example 4

[0090] The specific implementation method is the same as that of Example 1, except that the fermentation order of Bacillus licheniformis and Clostridium butyricum is switched when preparing the Polygonum multiflorum extract.

[0091] Comparative Example 5

[0092] The specific implementation is the same as that of Example 1, except that the Platycladus orientalis leaf extract is not added.

[0093] Comparative Example 6

[0094] The specific implementation is the same as that of Example 1, except that the Polygonum multiflorum extract is not added.

[0095] Comparative Example 7

[0096] The specific implementation is the same as that of Example 1, except that no ginseng root extract is added.

[0097] Test Example 1

[0098] 5α-reductase activity

[0099] When 5α-reductase activity is high, it promotes the conversion of more testosterone into DHT, stimulating the sebaceous glands to over-secrete sebum. When 5α-reductase activity is inhibited, the sebaceous glands reduce sebum secretion. This experiment tested the anti-hair loss compositions of Examples 1-3 and Comparative Examples 1-7 to demonstrate their oil-control effects.

[0100] The anti-hair loss compositions of Examples 1-3 and Comparative Examples 1-7 were diluted 5 times with deionized water respectively.

[0101] The negative control was PBS solution, and the positive control was finasteride, which was diluted with anhydrous ethanol to a concentration of 0.1% by mass.

[0102] Dilutions of the anti-hair loss compositions of Examples 1-3 and Comparative Examples 1-7, a negative control group, a positive control group, and a blank tube group were set up respectively. Different reagent solutions (5α-reductase crude enzyme + pH 5.5 Tris-HCl buffer + testosterone + test solution + NADPH) were added to each group, shaken well, and each group was subjected to HPLC analysis to determine the testosterone content in each tube. The change in testosterone peak area at 0 min before the reaction and 30 min after the reaction was used to reflect the activity inhibition rate of the test solution on 5α-reductase in the system. Specific results are shown in Table 1.

[0103] 5α-reductase inhibition rate = (1-testosterone content change value of the test group / testosterone content change value of the blank group) × 100%.

[0104] Table 1 5α-reductase activity inhibition rate in each group

[0105]

[0106]

[0107] As shown in Table 1, the anti-hair loss compositions prepared using the methods of Examples 1-3 of the present invention significantly increased 5α-reductase inhibition compared to the negative control, achieving excellent anti-hair loss efficacy. The data from Example 1 and Comparative Examples 1-4 demonstrate that varying the enzyme type, enzymatic hydrolysis sequence, fermentation microorganism type, and fermentation sequence significantly impacts 5α-reductase inhibition. The data from Example 1 and Comparative Examples 5-7 demonstrate that Platycladus orientalis leaf extract, Panax ginseng root extract, and Polygonum multiflorum extract significantly impact 5α-reductase inhibition.

[0108] Test Example 2

[0109] The Hamilton grading system divides hair loss into 7 levels, each with different manifestations. The higher the level, the more severe the hair loss:

[0110] Grade I: The anterior hairline is normal with only very slight receding of the temporal part;

[0111] Grade II: Mild receding of the temporal region with thinning of the hair at the forehead hairline;

[0112] Grade III: The amount of hair loss reaches the minimum standard of the definition of hair loss, with obvious hair receding on the forehead, leaving only a small amount or almost no hair;

[0113] Grade III: Vertex type, hair loss is limited to the top of the head, and the hair on the forehead is obviously receding (the degree of receding is lighter than Grade II);

[0114] Grade IV: The hairline on the forehead and temples is significantly receding, the hair becomes thinner, and the hair on the top of the head is sparse, or even bald;

[0115] Grade V: Large bald areas on the front and top of the scalp, which are more severe than Grade IV. The junction between the top and the forehead is very narrow, with only sparse hair.

[0116] Grade VI: The junction between the top and the forehead disappears, and the baldness spreads to the sides and back;

[0117] Grade VI I: Only a narrow horseshoe-shaped hair growth area remains on both sides of the scalp and on the occipital region. This hair growth area extends from the front of the ear to the low position of the occipital region, and is symmetrical on both sides. However, the hair in front of the ear recedes backward and downward.

[0118] According to the Hamilton hair loss scale, 100 male subjects meeting Hamilton hair loss scale grades III-IV were selected and divided into two groups. One group used the anti-hair loss composition of Example 1 of the present invention, and the other group used minoxidil tincture. There was no significant difference in age or Hamilton hair loss scale between the two groups.

[0119] The method for using the anti-hair loss composition of Example 1 of the present invention is as follows: after washing and drying the hair, dilute the anti-hair loss composition of Example 1 5 times with water, and evenly spray 1.2 ml of the diluted anti-hair loss composition on the hair loss area of ​​the scalp. After spraying, gently massage the scalp with the fingertips for 5 minutes, once in the morning and once in the evening.

[0120] The method of using minoxidil tincture is: after washing and drying the hair, apply 1mL of minoxidil tincture to the hair loss area of ​​the scalp. After application, gently massage the scalp with the fingertips for 5 minutes, once in the morning and once in the evening.

[0121] Efficacy criteria:

[0122] Recovery: Hair growth in the hair loss area returns to normal, and the density and thickness of the hair are basically the same as before hair loss;

[0123] Significantly effective: The hair volume increases significantly, with the density increasing by more than 50%, or the hair diameter thickens by more than 50%, and more new terminal hair grows;

[0124] Effective: Hair loss is controlled to a certain extent, hair thinning is improved, hair density increases by 15%-50%, or hair diameter thickens by 15%-50%, with a certain amount of new vellus hair or a small amount of terminal hair growing;

[0125] Ineffective: There is no significant improvement in hair loss, the increase in hair density is not obvious, and a large amount of hair loss continues, or only a small amount of vellus hair grows and falls off quickly, and the overall appearance is basically the same as before treatment.

[0126] Total effective rate = (cured cases + markedly effective cases + effective cases) / 50×100%.

[0127] After 2 months of use, the efficacy of each group was statistically analyzed, as shown in Table 2.

[0128] Table 2 Statistics of the anti-hair loss effect of each group

[0129]

[0130]

[0131] As can be seen from the data in Table 2, the anti-hair loss composition of the present invention is comparable to minoxidil tincture in terms of anti-hair loss effect.

[0132] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. An anti-hair loss composition containing Platycladus orientalis leaves, characterized in that The invention is composed of the following components in parts by weight: 15-25 parts of Platycladus orientalis leaf extract, 8-12 parts of ginseng root extract, 10-20 parts of chrysanthemum intestinal extract, 10-20 parts of Polygonum multiflorum extract, 5-10 parts of tea seed extract, 5-10 parts of sapodilla fruit extract, 5-10 parts of mulberry bark extract, 3-8 parts of Dictamni root bark extract, 3-8 parts of Cnidium monnieri seed extract and 2-5 parts of mint extract; The Platycladus orientalis leaf extract is obtained by enzymatic hydrolysis of serrapeptase and nattokinase: dried Platycladus orientalis leaves are crushed through a 100-200 mesh sieve, mixed with water at a solid-liquid ratio of 1:8-12 g / ml, 0.3%-0.5% of serrapeptase by weight of the Platycladus orientalis leaves and 0.1%-0.4% of nattokinase by weight of the Platycladus orientalis leaves are added, and enzymatic hydrolysis is carried out at 40-50° C. and a pH value of 7.5-8 for 1-3 hours, and the filtrate is filtered to obtain the Platycladus orientalis leaf extract, wherein the enzymatic activity of the serrapeptase is 20,000-50,000 U / g, and the enzymatic activity of the nattokinase is 50,000-100,000 U / g; The ginseng root extract is obtained by enzymatic hydrolysis of cellulase and snail protease in sequence: dried ginseng root is crushed through a 100-200 mesh sieve, mixed with water at a solid-liquid ratio of 1:10-15 g / ml, 0.4%-0.8% of the weight of the ginseng root is added to the mixture, and enzymatic hydrolysis is carried out at 45-55° C. and a pH value of 4.5-5.5 for 3-5 hours, 0.3%-0.5% of the weight of the ginseng root is added to the mixture, and enzymatic hydrolysis is carried out at 33-37° C. and a pH value of 5.5-7 for 1-3 hours, and the filtrate is filtered to obtain the ginseng root extract, wherein the enzymatic activity of the cellulase is 100,000-200,000 U / g, and the enzymatic activity of the snail protease is 400,000-500,000 U / g; The snakehead intestine extract is obtained by fermenting Bifidobacterium longum and Lactobacillus delbrueckii subsp. bulgaricus: dried snakehead intestine is crushed and passed through an 80-100 mesh sieve, mixed with water at a solid-liquid ratio of 1:12-15 g / ml, inoculated with 1%-3% of Bifidobacterium longum by weight of the snakehead intestine and 0.8%-1.5% of Lactobacillus delbrueckii subsp. bulgaricus by weight of the snakehead intestine, fermented at 33-38° C. and a pH value of 6.2-7 for 2-4 days, filtered with a 0.22-0.45 μm filter membrane, and sterilized to obtain a filtrate as the snakehead intestine extract; the Bifidobacterium longum is CCTCC NO: M2019028, with a bacterial activity of 1.3 billion to 1.8 billion CFU / g, and the Lactobacillus delbrueckii subsp. bulgaricus is CCTCC NO: M 2019203, with a bacterial activity of 2 billion to 3 billion CFU / g; The Polygonum multiflorum extract is obtained by fermenting Bacillus licheniformis and Clostridium butyricum in sequence: dried Polygonum multiflorum is crushed through an 80-100 mesh sieve, mixed with water at a solid-liquid ratio of 1:15-20 g / ml, inoculated with 0.5%-1.5% of the weight of the Polygonum multiflorum by Bacillus licheniformis, aerobically fermented at 35-40° C. and pH 6.5-7 for 1-3 days, inoculated with 1%-3% of the weight of the Polygonum multiflorum by Clostridium butyricum, fermented at 33-38° C. and pH 5.5-6.5 for 2-4 days, filtered with a 0.22-0.45 μm filter membrane, and sterilized to obtain the filtrate as the Polygonum multiflorum extract; the Bacillus licheniformis is Bacillus licheniformis CICC ® 10103, bacterial activity is 2 billion to 4 billion CFU / g, the butyric acid Clostridium is Clostridium butyricum CICC ® 24854, bacterial activity is 300 million-800 million CFU / g.

2. The anti-hair loss composition according to claim 1, characterized in that The tea seed extract, mulberry bark extract, Dictamni root bark extract and Cnidium monnieri seed extract are obtained by extraction with ethanol solution.

3. The anti-hair loss composition according to claim 1, characterized in that The soapberry extract is obtained by water extraction.

4. The anti-hair loss composition according to claim 1, characterized in that The mint extract is obtained by distillation.

5. The method for preparing the anti-hair loss composition according to any one of claims 1 to 4, characterized in that: include: The Platycladus orientalis leaf extract, ginseng root extract, chrysanthemum intestinal extract, Polygonum multiflorum extract, tea seed extract, sapodilla fruit extract, mulberry bark extract, Dictamni root bark extract, Cnidium monnieri seed extract and mint extract are mixed in parts by weight and sterilized to obtain the anti-hair loss composition.

6. Use of the anti-hair loss composition according to any one of claims 1 to 4 in the preparation of an anti-hair loss product.

7. The use according to claim 6, characterized in that The anti-hair loss product is an external preparation for skin use for topical application.

8. The use according to claim 7, characterized in that The anti-hair loss product can inhibit the activity of 5α-reductase and alleviate seborrheic alopecia.

Images