Fertilizer synergist, water-soluble fertilizer as well as preparation method and application of water-soluble fertilizer

Abstract

The invention provides a fertilizer synergist, a water-soluble fertilizer and a preparation method and application thereof.The fertilizer synergist comprises a nitrogen-containing compound and a biostimulant, and the preparation method of the nitrogen-containing compound comprises the steps that bromobenzene and aniline are subjected to a first reaction to obtain a first reaction product; and carrying out second reaction on the first reaction product and anhydride to obtain the nitrogen-containing compound. The fertilizer synergist provided by the invention can improve the adaptive capacity of crops to saline-alkali stress and promote high-quality growth and yield increase of the crops.

Description

Technical Field

[0001] The present application relates to the field of fertilizers, and in particular to fertilizer synergists, water-soluble fertilizers, and preparation methods and applications thereof. Background Art

[0002] With global climate change and inappropriate land management, soil salinization has become one of the important factors restricting crop production. Salt-alkali stress has a negative impact on the growth and development of crops, leading to reduced yield, reduced quality and even death. Therefore, improving crop resistance to salt-alkali stress has become an important issue in agricultural production.

[0003] At present, the methods for improving crop salt resistance mainly include breeding improvement, soil improvement and application of exogenous substances. However, traditional breeding methods have long cycles and low efficiency, soil improvement requires a lot of investment costs, and the application of exogenous substances is limited by safety and environmental friendliness.

[0004] Therefore, it is of great significance to find a new, efficient and low-cost crop salt resistance enhancer. Summary of the invention

[0005] The present application aims to solve the technical problems existing in the prior art at least to a certain extent. To this end, the present application proposes a fertilizer synergist, a water-soluble fertilizer, and a preparation method and application thereof. The fertilizer synergist of the present application can improve the adaptability of crops to saline-alkali stress, promote high-quality growth of crops and increase yield.

[0006] In a first aspect of the present application, the present application proposes a fertilizer synergist. According to an embodiment of the present application, the fertilizer synergist comprises a nitrogen-containing compound and a biostimulant, and the preparation method of the nitrogen-containing compound comprises:

[0007] Conducting a first reaction between bromobenzene and aniline to obtain a first reaction product;

[0008] The first reaction product is subjected to a second reaction with an acid anhydride to obtain the nitrogen-containing compound.

[0009] According to the fertilizer synergist of the embodiment of the present application, the nitrogen-containing compound and the biostimulant work together to regulate the physiological metabolic process in the crop body, improve the crop's tolerance to salt, and reduce the negative impact of saline-alkali stress on crop growth. At the same time, it can also promote the healthy development of the crop root system and enhance the crop's ability to absorb water and nutrients, thereby achieving higher quality growth in saline-alkali soil. In addition, it can also promote crop photosynthesis and increase crop yields.

[0010] According to the embodiments of the present application, the above-mentioned fertilizer synergist may also have the following additional technical features:

[0011] According to an embodiment of the present application, the first reaction comprises:

[0012] The bromobenzene, aniline and alkaline catalyst are heated to react, and after the reaction is completed, the pH value of the obtained mixed solution is adjusted, and the organic phase is collected to obtain the first reaction product;

[0013] The heating reaction is carried out at a temperature of 50°C to 90°C and for a time of 1h to 4h.

[0014] According to an embodiment of the present application, the alkaline catalyst includes at least one of sodium carbonate, potassium hydroxide and potassium carbonate.

[0015] According to an embodiment of the present application, the reagent for adjusting the pH value of the obtained mixed solution includes water, and the pH value of the obtained mixed solution is adjusted to 6-8.

[0016] According to an embodiment of the present application, the second reaction comprises:

[0017] The first reaction product, anhydride and an organic solvent are stirred for reaction, and after the reaction is completed, the pH value of the obtained mixed solution is adjusted, and the organic phase is collected;

[0018] The stirring reaction is carried out at a temperature of 60°C to 100°C and for a time of 1h to 4h.

[0019] According to an embodiment of the present application, the organic solvent includes ethyl acetate.

[0020] According to an embodiment of the present application, the reagent for adjusting the pH value of the obtained mixed solution includes water, and the pH value of the obtained mixed solution is adjusted to 6-8.

[0021] According to an embodiment of the present application, the method further comprises: purifying the second reaction product obtained by the second reaction to obtain the nitrogen-containing compound;

[0022] The purification treatment comprises: injecting the second reaction product into a silica gel column, eluting with an eluent, and collecting the eluate to obtain the nitrogen-containing compound.

[0023] According to an embodiment of the present application, the eluent comprises 80% to 100% by volume ethyl acetate aqueous solution.

[0024] According to an embodiment of the present application, the elution flow rate is 0.5 mL / min to 2.0 mL / min.

[0025] According to an embodiment of the present application, the biostimulant includes humic acid, amino acid and sodium indoleacetic acid.

[0026] According to an embodiment of the present application, the biostimulant includes: humic acid, methionine, glycine and sodium indoleacetic acid.

[0027] According to an embodiment of the present application, the mass ratio of humic acid, methionine, glycine and sodium indoleacetic acid is (4-6): (0.1-3): (0.1-3): (0.1-5): (2-6).

[0028] According to an embodiment of the present application, the mass ratio of the nitrogen-containing compound to the biostimulant is (2-6):(4-25).

[0029] According to an embodiment of the present application, the oxygen content of the fertilizer synergist is less than 16 mg / L.

[0030] According to an embodiment of the present application, the fertilizer synergist further includes a solvent, and the solvent includes water, ethanol and glycerol.

[0031] According to an embodiment of the present application, the mass ratio of water, ethanol and glycerol is (0.1-3):(1-5):(0.1-3).

[0032] In a second aspect of the present application, the present application provides a water-soluble fertilizer. According to an embodiment of the present application, the water-soluble fertilizer comprises: a basic fertilizer and the fertilizer synergist described in the first aspect.

[0033] According to an embodiment of the present application, the mass ratio of nitrogen content, phosphorus content and potassium content in the basic fertilizer is (0-2): (1-3): (1-3).

[0034] According to an embodiment of the present application, the basic fertilizer includes at least one of ammonium sulfate, urea, potassium nitrate and potassium dihydrogen phosphate.

[0035] According to an embodiment of the present application, the mass ratio of the fertilizer synergist to the basic fertilizer is (1-3):(5-7).

[0036] In a third aspect of the present application, the present application proposes a method for preparing the water-soluble fertilizer according to the first aspect. According to an embodiment of the present application, the method comprises: mixing the nitrogen-containing compound, the biostimulant and the basic fertilizer.

[0037] According to an embodiment of the present application, the method includes:

[0038] Performing a first mixing process on the nitrogen-containing compound, the biostimulant and a portion of the solvent to obtain a mixed material;

[0039] Deoxygenating the mixture to obtain a deoxygenated mixture;

[0040] The deoxygenated mixed material, the basic fertilizer and the remaining part of the solvent are subjected to a second mixing treatment to obtain the water-soluble fertilizer.

[0041] According to an embodiment of the present application, the deoxygenation treatment includes: filling an inert gas into a container containing the mixed material or heating the mixed material to reduce the oxygen content in the mixed material to less than 16 mg / L.

[0042] In the fourth aspect of the present application, the present application proposes the use of the fertilizer enhancer described in the first aspect, the water-soluble fertilizer described in the second aspect, or the water-soluble fertilizer prepared by the method described in the third aspect in cultivating crops or improving the salt-alkali resistance of crops.

[0043] In a fifth aspect of the present application, the present application proposes a method for cultivating crops or improving the salt-alkali resistance of crops. According to an embodiment of the present application, the method comprises: contacting the water-soluble fertilizer described in the second aspect or the water-soluble fertilizer prepared by the method described in the third aspect with crop seeds or crops during growth.

[0044] According to an embodiment of the present application, during seed treatment, the method includes: soaking the crop seeds with the water-soluble fertilizer;

[0045] And / or, during the growth period, the method includes: applying the water-soluble fertilizer to the leaves of the crop, or applying it through root irrigation.

[0046] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0048] Figure 1 A flow chart of a method for preparing a nitrogen-containing compound according to one embodiment of the present application is shown. DETAILED DESCRIPTION

[0049] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be understood as limiting the present invention.

[0050] It should be noted that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. Further, in the description of the present invention, unless otherwise specified, the meaning of "plurality" is two or more.

[0051] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0052] In this document, the terms “include” or “comprising” are open expressions, that is, including the contents specified in the present invention but not excluding other contents.

[0053] As used herein, the terms "optionally", "optional" or "optionally" generally mean that the subsequently described event or circumstance may but need not occur, and that the description includes instances where the event or circumstance occurs and instances where it does not.

[0054] At present, the methods for improving the salt resistance of crops mainly include breeding improvement, soil improvement and application of exogenous substances. However, the traditional breeding method has a long cycle and low efficiency, soil improvement requires a large amount of investment cost, and the application of exogenous substances is limited by safety and environmental friendliness. The large-scale use of chemical fertilizers in the planting process will cause soil compaction and salinization, resulting in a decrease in soil fertility and a decrease in fertilizer utilization. At the same time, crop diseases increase significantly and the quality of agricultural products decreases. The salt-alkali resistant water-soluble fertilizers proposed in the prior art are mostly humic acid, composite microorganisms, etc., and their materials and methods have various problems, such as high cost and unclear effects.

[0055] In view of this, the present application proposes a novel nitrogen-containing compound, which, when used in combination with a biostimulant, has a good salt-alkali resistance effect and can promote high-quality growth and increase yield of crops in a saline-alkali environment.

[0056] To this end, in the first aspect of the present application, the present application proposes a fertilizer synergist. According to an embodiment of the present application, the fertilizer synergist includes a nitrogen-containing compound and a biostimulant, see Figure 1 The preparation method of the nitrogen-containing compound comprises: S100 a first reaction, S200 a second reaction and S300 a purification treatment. Each step will be described in detail below.

[0057] S100 First Response

[0058] In this step, bromobenzene and aniline are subjected to a first reaction to obtain a first reaction product. The lone pair of electrons on the nitrogen atom of aniline attacks the aromatic ring of bromobenzene, a nucleophilic substitution reaction occurs, and the bromine atom is replaced by the amino group on aniline to generate p-aminophenyl ether.

[0059] According to an embodiment of the present application, the bromobenzene, aniline and alkaline catalyst are heated to react, and after the reaction is completed, the pH value of the obtained mixed solution is adjusted, and the organic phase is collected to obtain the first reaction product.

[0060] According to an embodiment of the present application, the temperature of the heating reaction is 50°C to 90°C, for example, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C, 90°C; the time is 1h to 4h, for example, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h. Thus, the reaction rate can be increased while avoiding side reactions or substrate decomposition that may be caused by excessive temperature. In addition, the reactants are fully converted to reduce unnecessary by-products due to overreaction. Thus, it is helpful to improve the yield and purity of the target product and reduce energy consumption and waste of raw materials.

[0061] According to an embodiment of the present application, the alkaline catalyst includes at least one of sodium carbonate, potassium hydroxide and potassium carbonate, thereby providing an alkaline environment for the reaction, accelerating the reaction rate, and promoting a rapid and sufficient reaction.

[0062] According to an embodiment of the present application, the reagent for adjusting the pH value of the obtained mixed solution includes water, and the pH value of the obtained mixed solution is adjusted to 6 to 8. This facilitates the subsequent reaction.

[0063] S200 Second Response

[0064] In this step, the first reaction product is subjected to a second reaction with an acid anhydride to obtain a nitrogen-containing compound. The acid anhydride, as an electrophilic reagent, undergoes an electrophilic substitution reaction with the amino group on the structure of the nitrogen-containing compound to form a new amide bond or ester bond, thereby obtaining the nitrogen-containing compound.

[0065] According to an embodiment of the present application, the second reaction comprises: stirring the first reaction product, anhydride and an organic solvent to react, and after the reaction is completed, adjusting the pH value of the obtained mixed solution and collecting the organic phase.

[0066] According to an embodiment of the present application, the temperature of the stirring reaction is 60°C to 100°C, for example, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C, 90°C, 95°C, 100°C; the time is 1h to 4h, for example, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h. Thus, the reaction rate can be increased while avoiding side reactions or substrate decomposition that may be caused by excessive temperature. In addition, the reactants are fully converted to reduce unnecessary by-products due to overreaction. Thus, it is helpful to improve the yield and purity of the target product and reduce energy consumption and waste of raw materials.

[0067] According to an embodiment of the present application, the organic solvent includes ethyl acetate, thereby allowing both the reactants and the products to be fully dissolved, promoting the smooth progress of the reaction, and also helping to improve the quality and yield of the product.

[0068] According to an embodiment of the present application, the reagent for adjusting the pH value of the obtained mixed solution includes water, and the pH value of the obtained mixed solution is adjusted to 6 to 8. This facilitates subsequent purification treatment.

[0069] S300 purification treatment

[0070] In this step, the second reaction product obtained by the second reaction is purified to obtain the nitrogen-containing compound.

[0071] According to an embodiment of the present application, the purification process comprises:

[0072] The second reaction product is injected into a silica gel column, eluted with an eluent, and the eluent is collected to obtain the nitrogen-containing compound; the eluent includes 80% to 100% by volume of ethyl acetate aqueous solution, for example, 80%, 85%, 90%, 95%, or 100% by volume. Thus, the target nitrogen-containing compound having a high salt-alkali resistance effect and promoting crop growth and increasing yield can be separated, and the target nitrogen-containing compound has high purity and yield.

[0073] According to an embodiment of the present application, the elution flow rate is 0.5 mL / min to 2.0 mL / min, for example, 0.5 mL / min, 1.0 mL / min, 1.5 mL / min, 2.0 mL / min. Thus, the target nitrogen-containing compound having high salt-alkali resistance and promoting crop growth and yield increase can be separated, and the target nitrogen-containing compound has high purity and yield.

[0074] According to the embodiment of the present application, when the absorption peak appears, the eluate is collected to obtain the nitrogen-containing compound, thereby separating and obtaining the target nitrogen-containing compound having a high salt-alkali resistance effect and promoting crop growth and increasing yield, and the target nitrogen-containing compound has high purity and yield.

[0075] According to an embodiment of the present application, the biostimulant includes humic acid, amino acids and sodium indoleacetic acid, thereby improving soil structure, increasing soil water and fertilizer retention capacity, stimulating crop root development, and improving crop absorption efficiency of nutrients, thereby improving crop resistance to salt and alkali, promoting crop growth and increasing yield.

[0076] According to an embodiment of the present application, the amino acid includes at least one of methionine, glycine and proline, thereby further improving salt-alkali resistance and promoting crop growth and yield increase.

[0077] According to an embodiment of the present application, the biostimulant includes: humic acid, methionine, glycine and sodium indoleacetic acid; the mass ratio of humic acid, methionine, glycine and sodium indoleacetic acid is (4-6): (0.1-3): (0.1-3): (0.1-5): (2-6). Thereby, the bioavailability of the fertilizer synergist is further improved, and crops are promoted to absorb and utilize these nutrients more effectively, thereby enhancing the growth and development of crops. At the same time, it can further reduce the waste of nutrients and the potential negative impact on the environment, improve the overall effectiveness of fertilizers and the salt-alkali resistance of crops. In addition, it helps to further reduce production costs and reduce unnecessary excessive additions.

[0078] According to the embodiment of the present application, the mass ratio of the nitrogen-containing compound to the biostimulant is (2-6): (4-25). This can further improve the salt-alkali resistance of crops, promote crop growth and increase yield.

[0079] According to an embodiment of the present application, the solvent includes water, ethanol and glycerol

[0080] According to an embodiment of the present application, the fertilizer synergist further includes a solvent, and the solvent includes water, ethanol and glycerol, so that various nutrients are evenly dispersed therein, thereby improving their bioavailability and crop absorption efficiency.

[0081] According to the embodiment of the present application, the mass ratio of water, ethanol and glycerol is (0.1-3): (1-5): (0.1-3), so that various nutrients are evenly dispersed therein, thereby improving their bioavailability and crop absorption efficiency.

[0082] According to an embodiment of the present application, the oxygen content of the fertilizer synergist is less than 16 mg / L, for example, 0 mg / L, 1 mg / L, 2 mg / L, 4 mg / L, 5 mg / L, 6 mg / L, 8 mg / L, 10 mg / L, 12 mg / L, 14 mg / L, 16 mg / L. This helps to improve the physical properties of crops.

[0083] Water soluble fertilizer

[0084] In the second aspect of the present application, the present application proposes a water-soluble fertilizer. According to an embodiment of the present application, the water-soluble fertilizer includes: a basic fertilizer and the fertilizer synergist described in the first aspect. The water-soluble fertilizer of the present application can provide comprehensive nutrition for crops and promote their healthy growth. Moreover, it can improve the soil structure, increase the water and fertilizer retention capacity of the soil, and at the same time stimulate the development of the crop root system and improve the crop's absorption efficiency of nutrients. Furthermore, it can enhance the stress resistance of crops, such as drought resistance, salt and alkali resistance, etc., so that they can maintain a good growth state under adverse environmental conditions. As a result, it can promote the overall high-quality growth of crops and increase yields.

[0085] According to an embodiment of the present application, the mass ratio of nitrogen content, phosphorus content and potassium content in the basic fertilizer is (0-2): (1-3): (1-3), for example, it can be 10:30:20, 8:20:10, 20:10:30, 15:25:25, 10:10:10. Thus, balanced nutrition can be provided for crops to meet their needs for various main nutrients at different growth stages.

[0086] According to an embodiment of the present application, the mass ratio of the fertilizer synergist to the basic fertilizer is (1-3): (5-7), for example, 10:50, 20:70, 15:55, 15:65, 20:60, 15:60, 10:70, 30:50. Thus, balanced nutrition can be provided to crops to meet their needs for various main nutrients at different growth stages.

[0087] According to an embodiment of the present application, the basic fertilizer includes at least one of ammonium sulfate, urea, potassium nitrate and potassium dihydrogen phosphate.

[0088] According to an embodiment of the present application, the pH value of the water-soluble fertilizer is 4.0-6.5, for example, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, which helps to promote crop growth and improve salt-alkali resistance.

[0089] It should be noted that the characteristics and advantages described above for the fertilizer enhancer in the first aspect are also applicable to the water-soluble fertilizer and will not be repeated here.

[0090] Method for preparing water-soluble fertilizer

[0091] In a third aspect of the present application, the present application proposes a method for preparing the water-soluble fertilizer of the second aspect. According to an embodiment of the present application, the method comprises: mixing the nitrogen-containing compound, the biostimulant and the basic fertilizer to obtain the water-soluble fertilizer.

[0092] According to an embodiment of the present application, the method includes: subjecting the nitrogen-containing compound, the biostimulant and part of the solvent to a first mixing treatment to obtain a mixture; subjecting the mixture to a deoxygenation treatment to obtain a deoxygenated mixture; subjecting the deoxygenated mixture, the basic fertilizer and the remaining part of the solvent to a second mixing treatment to obtain the water-soluble fertilizer. Thus, the deoxygenation treatment is used to reduce the content of dissolved oxygen in the solution, prevent the oxidative degradation of certain active ingredients in the fertilizer, maintain its stability and biological activity, and thus ensure the quality and effect of the fertilizer. In addition, the deoxygenation treatment also helps to improve the physical properties of crops.

[0093] According to an embodiment of the present application, the deoxygenation treatment includes: filling an inert gas into a container containing the mixed liquid obtained by the mixing treatment or heating the mixed liquid so that the oxygen content in the biostimulant is less than 16 mg / L. Thus, the deoxygenation effect is exerted by filling an inert gas or performing a heating treatment, thereby improving the physical properties of the crop.

[0094] In some embodiments, the inert gas does not react with oxygen at room temperature, and after being dissolved in water, its aqueous solution is non-oxidizing. Exemplarily, the inert gas includes one or more of hydrogen, carbon dioxide and nitrogen.

[0095] It should be noted that the characteristics and advantages described above for the water-soluble fertilizer in the second aspect are also applicable to the method for preparing the water-soluble fertilizer and will not be repeated here.

[0096] Applications and methods

[0097] In the fourth aspect of the present application, the present application proposes the use of the fertilizer synergist described in the first aspect, the water-soluble fertilizer described in the second aspect, or the water-soluble fertilizer prepared by the method described in the third aspect in cultivating crops or improving the salt-alkali resistance of crops. As mentioned above, the fertilizer synergist of the present application can regulate the physiological metabolic process in the crop body, improve the tolerance of crops to salt, and reduce the negative impact of salt-alkali stress on crop growth. At the same time, it can also promote the healthy development of crop roots and enhance the crop's ability to absorb water and nutrients, thereby achieving higher quality growth in saline-alkali soils. In addition, it can also promote the photosynthesis of crops and increase crop yields.

[0098] In the fifth aspect of the present application, the present application proposes a method for cultivating crops or improving the salt-alkali resistance of crops. According to an embodiment of the application, the method comprises: contacting the water-soluble fertilizer described in the second aspect or the water-soluble fertilizer prepared by the method described in the third aspect with crop seeds or crops during growth. Thus, the method of the present application can regulate the physiological metabolic processes in the crop body, improve the crop's tolerance to salt, and reduce the negative impact of salt-alkali stress on crop growth. At the same time, it can also promote the healthy development of crop roots and enhance the crop's ability to absorb water and nutrients, thereby achieving higher quality growth in saline-alkali soils. In addition, it can also promote crop photosynthesis and increase crop yields.

[0099] According to an embodiment of the present application, during seed treatment, the method includes: soaking the crop seeds with the water-soluble fertilizer. As a result, the seeds can absorb sufficient water and nutrition before sowing, which enhances the vitality and germination rate of the seeds, accelerates the germination speed and promotes the development of the root system. In addition, it can also help the seedlings to establish a stronger natural defense mechanism and improve resistance to diseases and insect pests. As a result, the seedlings can adapt to the soil environment earlier, accelerate the growth rate, and ultimately obtain higher crop yields and better quality.

[0100] According to an embodiment of the present application, during the growth period, the method includes: applying the water-soluble fertilizer to the leaves of the crop, or applying it through root irrigation. This helps to improve the utilization efficiency of the water-soluble fertilizer, reduce fertilizer waste and potential pollution to the environment, and can also enhance the photosynthesis of the crop and improve the salt-alkali resistance of the crop.

[0101] It should be noted that the characteristics and advantages described above for the water-soluble fertilizer prepared by the method described in the second aspect and the method described in the third aspect are also applicable to the application and method of cultivating crops or improving the salt-alkali resistance of crops, and will not be repeated here.

[0102] The scheme of the present invention will be explained below in conjunction with the embodiments. It will be appreciated by those skilled in the art that the following embodiments are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. Where specific techniques or conditions are not indicated in the embodiments, the techniques or conditions described in the literature in this area or the product specifications are used. The reagents or instruments used are not indicated by the manufacturer and are all conventional products that can be obtained commercially.

[0103] Example 1

[0104] In this embodiment, water-soluble fertilizer is prepared according to the following method:

[0105] 1. Mix 1 part by weight of methionine, 1 part by weight of glycine, 3 parts by weight of sodium indoleacetate and 10 parts by weight of solvent (the mass ratio of distilled water, alcohol and glycerol is 1:2:1) to obtain the biostimulant.

[0106] 2. Mix 15 parts by weight of biostimulant, 60 parts by weight of basic fertilizer and 25 parts by weight of solvent to obtain water-soluble fertilizer with a pH value of 6.5. The fertilizer includes ammonium sulfate, urea, potassium nitrate and potassium dihydrogen phosphate, and the mass ratio of nitrogen, phosphorus and potassium is 10:30:20.

[0107] Example 2

[0108] The difference from Example 1 is that step 1 further comprises: subjecting the mixed liquid to the following deoxygenation treatment:

[0109] The mixed solution is heated to 80°C, maintained for 30 minutes, and then cooled to room temperature to obtain the biostimulant, in which the oxygen content is 8 mg / L.

[0110] Example 3

[0111] In this embodiment, water-soluble fertilizer is prepared according to the following method:

[0112] 1. Mix 1 part by weight of methionine, 1 part by weight of glycine, 2.9 parts by weight of nitrogen-containing compounds, 0.1 parts by weight of sodium indoleacetate and 10 parts by weight of solvent (the mass ratio of distilled water, alcohol and glycerol is 1:2:1) to obtain 15 parts by weight of fertilizer synergist with a pH value of 6.5.

[0113] 2. Mix 15 parts by weight of fertilizer synergist, 60 parts by weight of basic fertilizer (same as in Example 1) and 27.9 parts by weight of solvent to obtain a water-soluble fertilizer.

[0114] Example 4

[0115] The difference from Example 3 is that step 1 further comprises: subjecting the mixed liquid to the following deoxygenation treatment:

[0116] The mixed solution is heated to 80°C, maintained for 30 minutes, and then cooled to room temperature to obtain a fertilizer enhancer. The oxygen content in the biostimulant is 8 mg / L.

[0117] Example 5

[0118] In this embodiment, water-soluble fertilizer is prepared according to the following method:

[0119] 1. Mix 1 part by weight of methionine, 1 part by weight of glycine, 5 parts by weight of humic acid, 0.1 part by weight of sodium indoleacetate, 2.9 parts by weight of nitrogen-containing compounds and 10 parts by weight of solvent (the mass ratio of distilled water, alcohol and glycerol is 1:2:1), heat the resulting mixture to 80°C and keep it warm for 30 minutes to obtain 20 parts by weight of fertilizer synergist with an oxygen content of 8 mg / L.

[0120] 2. Mix 20 parts by weight of fertilizer synergist, 60 parts by weight of basic fertilizer (same as in Example 1) and 27.9 parts by weight of solvent to obtain a water-soluble fertilizer with a pH value of 6.5.

[0121] Example 6

[0122] In this embodiment, water-soluble fertilizer is prepared according to the following method:

[0123] 1. Mix 1 part by weight of methionine, 1 part by weight of glycine, 5 parts by weight of humic acid, 0.1 parts by weight of sodium indoleacetate and 10 parts by weight of solvent (the mass ratio of distilled water, alcohol and glycerol is 1:2:1), heat the resulting mixture to 80°C and keep it warm for 30 minutes to obtain 17.1 parts by weight of biostimulant with an oxygen content of 8 mg / L.

[0124] 2. Mix 17.1 parts by weight of biostimulant, 60 parts by weight of basic fertilizer (same as in Example 1) and 27.9 parts by weight of solvent to obtain a water-soluble fertilizer with a pH value of 6.5.

[0125] Example 7

[0126] In this embodiment, water-soluble fertilizer is prepared according to the following method:

[0127] 1. Mix 1 part by weight of methionine, 1 part by weight of glycine, 2.9 parts by weight of humic acid, 0.1 part by weight of sodium indoleacetate and 10 parts by weight of solvent (the mass ratio of distilled water, alcohol and glycerol is 1:2:1), heat the resulting mixture to 80°C and keep it warm for 30 minutes to obtain 15 parts by weight of biostimulant with an oxygen content of 8 mg / L.

[0128] 2. Mix 15 parts by weight of biostimulant, 60 parts by weight of basic fertilizer (same as in Example 1) and 27.9 parts by weight of solvent to obtain a water-soluble fertilizer with a pH value of 6.5.

[0129] Example 8

[0130] In this embodiment, water-soluble fertilizer is prepared according to the following method:

[0131] 1. Mix 1 part by weight of methionine, 1 part by weight of glycine, 5.1 parts by weight of nitrogen-containing compounds, 2.9 parts by weight of sodium indoleacetate and 10 parts by weight of solvent (the mass ratio of distilled water, alcohol and glycerol is 1:2:1), heat the resulting mixture to 80°C and keep it warm for 30 minutes to obtain 20 parts by weight of fertilizer synergist with an oxygen content of 8 mg / L.

[0132] 2. Mix 20 parts by weight of fertilizer synergist, 60 parts by weight of basic fertilizer (same as in Example 1) and 25.1 parts by weight of solvent to obtain a water-soluble fertilizer with a pH value of 6.5.

[0133] Test Case

[0134] 1. The water-soluble fertilizers prepared in Examples 1 to 4 were stored at 54° C. for 14 days, and the colors of the water-soluble fertilizers before and after storage were observed and tested. The results are shown in Table 1.

[0135] As can be seen from Table 1, water-soluble fertilizers that have not been deoxygenated will change color after a long time, while biostimulants that have been deoxygenated by heating will basically not change color, indicating that deoxygenation treatment can effectively prevent the oxidation and denaturation of active ingredients in water-soluble fertilizers.

[0136] Table 1 Water-soluble fertilizer colors

[0137] Initial Color Final color Example 1 Light yellow brown Example 2 Light yellow Light yellow Example 3 Light yellow brown Example 4 Light yellow Light yellow

[0138] 2. Greenhouse Experiment

[0139] 1. Experimental location: Greenhouse of Sinochem Agriculture Linyi R&D Center

[0140] 2. Experimental crops: corn

[0141] 3. When planting corn in a greenhouse, spray fertilizer on the leaves of corn at the small trumpet stage, with a spraying amount of 50 ml / mu. The treatment methods in different areas are different, as follows:

[0142] Experimental group 1: normal soil, sprayed with the water-soluble fertilizer of Example 1.

[0143] Experimental group 2: normal soil, sprayed with the water-soluble fertilizer of Example 2.

[0144] Experimental group 3: normal soil, sprayed with the water-soluble fertilizer of Example 3.

[0145] Experimental group 4: normal soil, sprayed with the water-soluble fertilizer of Example 4.

[0146] Experimental group 5: normal soil, sprayed with the water-soluble fertilizer of Example 5.

[0147] Experimental group 6: normal soil, sprayed with the water-soluble fertilizer of Example 6.

[0148] Experimental group 7: normal soil, sprayed with the water-soluble fertilizer of Example 7.

[0149] Experimental group 8: saline-alkali soil (salt content 5‰), sprayed with the water-soluble fertilizer of Example 8.

[0150] Experimental group 9: saline-alkali soil (salt content 5‰), sprayed with the water-soluble fertilizer of Example 2.

[0151] Experimental group 10: saline-alkali soil (salt content 5‰), sprayed with the water-soluble fertilizer of Example 4.

[0152] Experimental group 11: saline-alkali soil (salt content 5‰), sprayed with the water-soluble fertilizer of Example 5.

[0153] Experimental group 12: saline-alkali soil (salt content 5‰), sprayed with the water-soluble fertilizer of Example 6.

[0154] Control group 1: normal soil with clean water.

[0155] Control group 2: clean water was applied to saline-alkali soil (salt content 5‰).

[0156] 21 days after spraying, the plant height, stem diameter, SPAD, 100-grain weight and yield of the cultivated corn were measured. The results are shown in Table 2.

[0157] Table 2 Corn growth and yield

[0158]

[0159]

[0160] It can be seen from Table 2 that compared with the control group applying clear water, the experimental groups 1 to 12 applying water-soluble fertilizers can provide more nutrients for corn growth, promote its growth, and improve its salt-alkali resistance, growth quality and yield.

[0161] Comparing Examples 2, 4, and 6, it can be seen that adding humic acid or novel nitrogen-containing compounds can improve the salt-alkali resistance of corn, promote the growth of corn in a saline-alkali environment, and increase plant height, stem diameter, SPAD, 100-grain weight, and yield. Comparing Examples 4, 5, and 7, it can be seen that compared with humic acid, nitrogen-containing compounds have a more prominent effect, and humic acid and nitrogen-containing compounds work together to achieve a better effect.

[0162] Comparing Examples 1 and 2, and Examples 3 and 4, it can be seen that deoxygenation has no significant effect on the growth-promoting effect of corn (100-grain weight and yield), but mainly affects its physical properties. Deoxygenation treatment can increase the plant height and stem thickness of corn.

[0163] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A fertilizer synergist, characterized in that: The invention comprises a nitrogen-containing compound and a biostimulant, and the preparation method of the nitrogen-containing compound comprises: Conducting a first reaction between bromobenzene and aniline to obtain a first reaction product; The first reaction product is subjected to a second reaction with an acid anhydride to obtain a nitrogen-containing compound.

2. The fertilizer synergist according to claim 1, characterized in that The first reaction includes: The bromobenzene, aniline and alkaline catalyst are heated to react, and after the reaction is completed, the pH value of the obtained mixed solution is adjusted, and the organic phase is collected to obtain the first reaction product; The heating reaction temperature is 50°C to 90°C and the time is 1h to 4h; Optionally, the alkaline catalyst comprises at least one of sodium carbonate, potassium hydroxide and potassium carbonate; Optionally, the reagent for adjusting the pH value of the obtained mixed solution includes water, and the pH value of the obtained mixed solution is adjusted to 6-8.

3. The fertilizer synergist according to claim 1, characterized in that The second reaction comprises: The first reaction product, anhydride and an organic solvent are stirred for reaction, and after the reaction is completed, the pH value of the obtained mixed solution is adjusted, and the organic phase is collected; The stirring reaction is carried out at a temperature of 60°C to 100°C and a time of 1h to 4h. Optionally, the organic solvent comprises ethyl acetate; Optionally, the reagent for adjusting the pH value of the obtained mixed solution includes water, and the pH value of the obtained mixed solution is adjusted to 6-8.

4. The fertilizer synergist according to claim 1, characterized in that Further including: Purifying the second reaction product obtained by the second reaction to obtain the nitrogen-containing compound; The purification treatment comprises: injecting the second reaction product into a silica gel column, eluting the silica gel column with an eluent, and collecting the eluate to obtain the nitrogen-containing compound; Optionally, the eluent comprises 80% to 100% by volume ethyl acetate aqueous solution; Optionally, the elution flow rate is 0.5 mL / min to 2.0 mL / min.

5. The fertilizer synergist according to claim 1, characterized in that: The biostimulant comprises humic acid, amino acid and sodium indoleacetic acid.

6. The fertilizer synergist according to claim 5, characterized in that The amino acid comprises at least one of methionine, glycine and proline; Optionally, the biostimulant comprises: humic acid, methionine, glycine and sodium indoleacetic acid; The mass ratio of humic acid, methionine, glycine and sodium indoleacetic acid is (4-6): (0.1-3): (0.1-3): (0.1-5): (2-6).

7. The fertilizer synergist according to claim 6, characterized in that The mass ratio of the nitrogen-containing compound to the biostimulant is (2-6):(4-25); Optionally, the oxygen content of the fertilizer synergist is less than 16 mg / L; Optionally, the fertilizer synergist further comprises a solvent, wherein the solvent comprises water, ethanol and glycerol; Optionally, the mass ratio of water, ethanol and glycerol is (0.1-3):(1-5):(0.1-3).

8. A water-soluble fertilizer, characterized in that: include: A basic fertilizer and the fertilizer synergist according to any one of claims 1 to 7.

9. The water-soluble fertilizer according to claim 8, characterized in that The mass ratio of nitrogen content, phosphorus content and potassium content in the basic fertilizer is (0-2): (1-3): (1-3); Optionally, the basic fertilizer includes at least one of ammonium sulfate, urea, potassium nitrate and potassium dihydrogen phosphate; Optionally, the mass ratio of the fertilizer synergist to the basic fertilizer is (1-3):(5-7).

10. A method for preparing the water-soluble fertilizer according to any one of claims 1 to 7, characterized in that: include: The nitrogen-containing compound, the biostimulant and the basic fertilizer are mixed.

11. The method according to claim 10, characterized in that include: Performing a first mixing process on the nitrogen-containing compound, the biostimulant and a portion of the solvent to obtain a mixed material; Deoxygenating the mixture to obtain a deoxygenated mixture; The deoxygenated mixture, the basic fertilizer and the remaining part of the solvent are subjected to a second mixing process to obtain the water-soluble fertilizer; Optionally, the deoxygenation treatment includes: filling an inert gas into a container containing the mixed material or heating the mixed material to reduce the oxygen content in the mixed material to less than 16 mg / L.

12. Use of the fertilizer synergist according to any one of claims 1 to 7, the water-soluble fertilizer according to claim 8 or 9, or the water-soluble fertilizer prepared by the method according to claim 10 or 11 in cultivating crops or improving the salt-alkali resistance of crops.

13. A method for cultivating crops or improving the salt-alkali resistance of crops, characterized in that: include: The water-soluble fertilizer according to claim 8 or 9 or the water-soluble fertilizer prepared by the method according to claim 10 or 11 is contacted with crop seeds or crops during growth.

14. The method according to claim 13, characterized in that During seed treatment, the method comprises: soaking the crop seeds with the water-soluble fertilizer; And / or, during the growth period, the method includes: applying the water-soluble fertilizer to the leaves of the crop, or applying it through root irrigation.

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